Sequence Characterization and Comparative Analysis of Three Plasmids Isolated from Environmental Vibrio Spp.ᰔ† Tracy H
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Chitin Metabolism by Vibrio Furnissii: Quantification of Nage Expression
Chitin Metabolism by Vibrio furnissii: Quantification of nagE Expression by Sarah G. Brown A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree Master of Science Major Subject: Interdisciplinary Sciences West Texas A&M University Canyon, Texas May 2017 Abstract The phosphoenolpyruvate: sugar phosphotransferase system (PTS) was first discovered in the 1960s by Kundig et al. The PTS is unique to bacteria, and is a rich area of study offering an abundance of potential research topics due to its environmental role and its potential as a target for future antibiotics. This study focuses on the nag operon, which plays an important role in chitin degradation. The expression of nagE, one gene located on the nag operon, was assessed via quantitative PCR (qPCR) in the presence of four substrates. This gene encodes the N-acetylglucosamine transporter protein. Expression of the gene was found to be up-regulated in the presence of N-acetylglucosamine, but not in the presence of glucose, mannose, or lactate. Potential future projects include: the quantification of expression of nagA via qPCR; the use of a reporter gene to quantify expression of nagE and nagA; study of NagC, thought to be the repressor of the nag operon; and further study and characterization of the gene encoding for the glucose specific transporter protein in V. furnissii. ii Acknowledgements I would like to thank Dr. Carolyn Bouma, first and foremost, for her invaluable guidance and expertise, as well as her time, patience, and encouragement during the course of this project. I would also like to thank Dr. Donna Byers, for time spent instructing me on how to carry out a gene expression study using qPCR, as well as the use of her Nanodrop system; Dr. -
Stony Brook University
SSStttooonnnyyy BBBrrrooooookkk UUUnnniiivvveeerrrsssiiitttyyy The official electronic file of this thesis or dissertation is maintained by the University Libraries on behalf of The Graduate School at Stony Brook University. ©©© AAAllllll RRRiiiggghhhtttsss RRReeessseeerrrvvveeeddd bbbyyy AAAuuuttthhhooorrr... Characterization of antimicrobial activity present in the cuticle of American lobster, Homarus americanus A Thesis Presented by Margaret Anne Mars to The Graduate School in Partial Fulfillment of the Requirements for the Degree of Master of Science in Marine and Atmospheric Science Stony Brook University December 2010 Stony Brook University The Graduate School Margaret Anne Mars We, the thesis committee for the above candidate for the Master of Science degree, hereby recommend acceptance of this thesis. Dr. Bassem Allam – Thesis Advisor Associate Professor School of Marine and Atmospheric Science Dr. Anne McElroy – Thesis Advisor Associate Professor School of Marine and Atmospheric Science Dr. Emmanuelle Pale Espinosa Adjunct Professor School of Marine and Atmospheric Science This thesis is accepted by the Graduate School Lawrence Martin Dean of the Graduate School ii Abstract of the Thesis Characterization of antimicrobial activity present in the cuticle of American lobster, Homarus americanus by Margaret Anne Mars Master of Science in Marine and Atmospheric Science Stony Brook University 2010 American lobster is an ecologically and socioeconomically important species. In recent years the species has been affected by disease and the catch in Southern New England has fallen dramatically. In order to fully understand how and why diseases affect lobster populations, it is imperative to fully understand lobster defense mechanisms. The cuticle, previously believed to act only as a physical barrier, has recently been shown to contain antimicrobial activity. -
The Impact of Environmental Changes on the Microbial Community Dynamics and Abundance of Pathogenic Vibrio Species in Coastal Ecosystems
The Impact of Environmental Changes on the Microbial Community Dynamics and Abundance of Pathogenic Vibrio species in Coastal Ecosystems by Candice Amber Thorstenson a Thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Marine Microbiology Approved Dissertation Committee _____________________________________ Prof. Dr. Matthias Ullrich Jacobs University Bremen Prof. Dr. Frank Oliver Glӧckner Jacobs University Bremen Dr. Mathias Wegner Alfred Wegener Institute for Polar and Marine Research Date of Defense: 26 August 2020 Department of Life Sciences and Chemistry i Table of Contents Summary .......................................................................................................... 1 General Introduction ........................................................................................ 3 The genus Vibrio ............................................................................................................. 5 Key Vibrio Characterization and Isolation Techniques .................................................. 9 Vibrio cholerae ............................................................................................................. 10 Vibrio parahaemolyticus ............................................................................................... 12 Vibrio vulnificus ............................................................................................................ 14 Genetic Modification Technologies Applied to Marine Bacteria ................................ -
Vibrios As Causal Agents of Zoonoses B
Vibrios as causal agents of zoonoses B. Austin To cite this version: B. Austin. Vibrios as causal agents of zoonoses. Veterinary Microbiology, Elsevier, 2010, 140 (3-4), pp.310. 10.1016/j.vetmic.2009.03.015. hal-00556049 HAL Id: hal-00556049 https://hal.archives-ouvertes.fr/hal-00556049 Submitted on 15 Jan 2011 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Accepted Manuscript Title: Vibrios as causal agents of zoonoses Author: B. Austin PII: S0378-1135(09)00119-9 DOI: doi:10.1016/j.vetmic.2009.03.015 Reference: VETMIC 4385 To appear in: VETMIC Received date: 9-1-2009 Revised date: 9-2-2009 Accepted date: 2-3-2009 Please cite this article as: Austin, B., Vibrios as causal agents of zoonoses, Veterinary Microbiology (2008), doi:10.1016/j.vetmic.2009.03.015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. -
Chelonia Mydas) from the Mexican Pacific
ABANICO VETERINARIO ISSN 2448-6132 abanicoacademico.mx/revistasabanico/index.php/abanico-veterinario Abanico Veterinario. January-December 2021; 11:1-13. http://dx.doi.org/10.21929/abavet2021.19 Original Article. Received: 13/12/2020. Accepted: 29/03/2021. Published: 12/04/2021. Code: e2020-101. Biochemical identification of potentially pathogenic and zoonotic bacteria in black turtles (Chelonia mydas) from the Mexican Pacific Identificación bioquímica de bacterias potencialmente patógenas y zoonóticas en las tortugas negras (Chelonia mydas) del Pacífico Mexicano Eduardo Reséndiz1, 2, 3 * ID , Helena Fernández-Sanz 2, 4 iD 1Departamento Académico de Ciencias Marinas y Costeras, Universidad Autónoma de Baja California Sur (UABCS). Carretera al Sur KM 5.5., Apartado Postal 19-B, C.P. 23080, La Paz B.C.S. México. 2Health assessments in sea turtles from Baja California Sur, La Paz B.C.S. México. 3Asociación Mexicana de Veterinarios de Tortugas A.C., Xalapa 91050, Veracruz, México. 4Posgrado en Ciencias Marinas y Costeras (CIMACO) UABCS, Carretera al Sur KM 5.5., Apartado Postal 19-B, C.P. 23080, La Paz B.C.S. México. Responsible author: Eduardo Reséndiz. *Author for correspondence: Eduardo Reséndiz. E-mail: [email protected], [email protected] ABSTRACT Sea turtles naturally have gastrointestinal microbiota; however, opportunistic behavior and pathogenicity of some bacteria have also been reported. Therefore, it is important to generate information on possible risks to turtles and human health. Five monthly field monitoring were carried out with captures of Chelonia mydas in the Ojo de Liebre lagoon complex. Physical examinations were performed and their morphometries were recorded; oral and cloacal swabs were made and sowing in McConkey and TCBS culture media. -
Phenotypic and Genotypic Properties of Vibrio Cholerae Non-O1, Non-O139 Isolates Recovered from Domestic Ducks in Germany
Characterization of Vibrio spp. isolates from Germany by reference to human clinical isolates using phenotypic and WGS-assisted genotypic methods Inaugural-Dissertation to obtain the academic degree Doctor rerum naturalium (Dr. rer. nat.) submitted to the Department of Biology, Chemistry, Pharmacy of Freie Universität Berlin by Keike Schwartz Berlin 2020 This dissertation was prepared under supervision of Dr. Eckhard Strauch at the Vibrio consiliary laboratory, Department Biological Safety, German Federal Institute for Risk Assessment. May 2016 to December 2020 First examiner: Prof. Dr. Karsten Nöckler Second examiner: Prof. Dr. Jens Rolff Disputation: 05/05/2021 Danksagung An dieser Stelle möchte ich all denjenigen danken, die mich auf meinem Weg zur Promotion begleitet haben. Mein größter Dank gilt Dr. Eckhard Strauch für die Ermöglichung und engagierte Betreuung dieser Doktorarbeit am Konsiliarlabor für Vibrionen sowie seine immerwährende uneingeschränkte Unterstützung. Ganz herzlich bedanke ich mich bei Prof. Dr. Karsten Nöckler und Prof. Dr. Jens Rolff für die Begutachtung dieser Arbeit. Mein besonderer Dank gilt auch Dr. Martin Richter für die Möglichkeit, diese kumulative Dissertation in seiner Fachgruppe anfertigen zu können. Ich danke Dr. Claudia Metelmann und allen Mitarbeitern des IMD Greifswald für die Bereitstellung aktueller klinischer Vibrio-Isolate aus Humanproben sowie Dr. Nadja Bier und Dr. Jens André Hammerl für die bereitwillige Weitergabe wertvoller Erfahrungen zu sequenzbasierten bioinformatischen Analysen. Besonders herzlich möchte ich mich bei Dr. Claudia Jäckel, Cornelia Göllner, Nicole vom Ort und Jonas Nekat aus dem Vibrio-Team für die angenehme, kollegiale Atmosphäre sowie ihre Hilfsbereitschaft und stetige Unterstützung bedanken. In diesem Zusammenhang sei auch allen übrigen Kolleginnen und Kollegen der Fachgruppe 45 für die schöne gemeinsame Zeit gedankt. -
Antibiotic-Resistant Bacteria and Gut Microbiome Communities Associated with Wild-Caught Shrimp from the United States Versus Im
www.nature.com/scientificreports OPEN Antibiotic‑resistant bacteria and gut microbiome communities associated with wild‑caught shrimp from the United States versus imported farm‑raised retail shrimp Laxmi Sharma1, Ravinder Nagpal1, Charlene R. Jackson2, Dhruv Patel3 & Prashant Singh1* In the United States, farm‑raised shrimp accounts for ~ 80% of the market share. Farmed shrimp are cultivated as monoculture and are susceptible to infections. The aquaculture industry is dependent on the application of antibiotics for disease prevention, resulting in the selection of antibiotic‑ resistant bacteria. We aimed to characterize the prevalence of antibiotic‑resistant bacteria and gut microbiome communities in commercially available shrimp. Thirty‑one raw and cooked shrimp samples were purchased from supermarkets in Florida and Georgia (U.S.) between March–September 2019. The samples were processed for the isolation of antibiotic‑resistant bacteria, and isolates were characterized using an array of molecular and antibiotic susceptibility tests. Aerobic plate counts of the cooked samples (n = 13) varied from < 25 to 6.2 log CFU/g. Isolates obtained (n = 110) were spread across 18 genera, comprised of coliforms and opportunistic pathogens. Interestingly, isolates from cooked shrimp showed higher resistance towards chloramphenicol (18.6%) and tetracycline (20%), while those from raw shrimp exhibited low levels of resistance towards nalidixic acid (10%) and tetracycline (8.2%). Compared to wild‑caught shrimp, the imported farm‑raised shrimp harbored -
CGM-18-001 Perseus Report Update Bacterial Taxonomy Final Errata
report Update of the bacterial taxonomy in the classification lists of COGEM July 2018 COGEM Report CGM 2018-04 Patrick L.J. RÜDELSHEIM & Pascale VAN ROOIJ PERSEUS BVBA Ordering information COGEM report No CGM 2018-04 E-mail: [email protected] Phone: +31-30-274 2777 Postal address: Netherlands Commission on Genetic Modification (COGEM), P.O. Box 578, 3720 AN Bilthoven, The Netherlands Internet Download as pdf-file: http://www.cogem.net → publications → research reports When ordering this report (free of charge), please mention title and number. Advisory Committee The authors gratefully acknowledge the members of the Advisory Committee for the valuable discussions and patience. Chair: Prof. dr. J.P.M. van Putten (Chair of the Medical Veterinary subcommittee of COGEM, Utrecht University) Members: Prof. dr. J.E. Degener (Member of the Medical Veterinary subcommittee of COGEM, University Medical Centre Groningen) Prof. dr. ir. J.D. van Elsas (Member of the Agriculture subcommittee of COGEM, University of Groningen) Dr. Lisette van der Knaap (COGEM-secretariat) Astrid Schulting (COGEM-secretariat) Disclaimer This report was commissioned by COGEM. The contents of this publication are the sole responsibility of the authors and may in no way be taken to represent the views of COGEM. Dit rapport is samengesteld in opdracht van de COGEM. De meningen die in het rapport worden weergegeven, zijn die van de auteurs en weerspiegelen niet noodzakelijkerwijs de mening van de COGEM. 2 | 24 Foreword COGEM advises the Dutch government on classifications of bacteria, and publishes listings of pathogenic and non-pathogenic bacteria that are updated regularly. These lists of bacteria originate from 2011, when COGEM petitioned a research project to evaluate the classifications of bacteria in the former GMO regulation and to supplement this list with bacteria that have been classified by other governmental organizations. -
Vibrios Annual Report 2018
Vibrios Annual Report 2018 Vibrios Cholera is a Class A Disease and must be reported to the state within 24 hours by calling the phone number listed on the web page. Non-cholera Vibrio infections are Class C Diseases and must be reported to the state within five business days. All Vibrio cultures should be sent to the State Public Health Laboratory for confirmation. Epidemiology All Vibrio species infections were added to the list of nationally notifiable diseases in January, 2007. Vibrios are Gram-negative, curved, rod-shaped bacteria that are natural inhabitants of the marine environment. In the United States, the transmission of Vibrio infection is primarily through the consumption of raw or under-cooked shellfish or by exposure of wounds to warm seawater or seafood drippings. The most common clinical presentation of Vibrio infection is self-limited gastroenteritis. Historically, many cases of Vibrio-associated gastroenteritis have been under-recognized. This is because most clinical laboratories do not routinely use the selective medium, thiosulfate-citrate-bile-salts-sucrose (TCBS) agar, for processing of stool specimens unless they are specifically requested to do so. However, the recent increase in the use of culture-independent diagnostic tests (CIDT) has led to an increase in diagnosed and reported cases. Wound infections and primary septicemia also occur, particularly for Vibrio vulnificus. Patients with liver disease and those who are immunocompromised are at a particularly high risk for significant morbidity and mortality associated with these infections. Early detection and initiation of treatment is very important, particularly for cholera and invasive Vibrio infections, because these infections may rapidly progress to death. -
Anti-Lipopolysaccharide Factors in the American Lobster Homarus Americanus: Molecular Characterization and Transcriptional Response to Vibrio Fluvialis Challenge
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by College of William & Mary: W&M Publish W&M ScholarWorks VIMS Articles Virginia Institute of Marine Science 2008 Anti-lipopolysaccharide factors in the American lobster Homarus americanus: Molecular characterization and transcriptional response to Vibrio fluvialis challenge KM Beale DW Towle N Jayasundara CM Smith JD Shields Virginia Institute of Marine Science See next page for additional authors Follow this and additional works at: https://scholarworks.wm.edu/vimsarticles Part of the Aquaculture and Fisheries Commons Recommended Citation Beale, KM; Towle, DW; Jayasundara, N; Smith, CM; Shields, JD; Small, HJ; and Greenwood, SJ, "Anti- lipopolysaccharide factors in the American lobster Homarus americanus: Molecular characterization and transcriptional response to Vibrio fluvialis challenge" (2008). VIMS Articles. 974. https://scholarworks.wm.edu/vimsarticles/974 This Article is brought to you for free and open access by the Virginia Institute of Marine Science at W&M ScholarWorks. It has been accepted for inclusion in VIMS Articles by an authorized administrator of W&M ScholarWorks. For more information, please contact [email protected]. Authors KM Beale, DW Towle, N Jayasundara, CM Smith, JD Shields, HJ Small, and SJ Greenwood This article is available at W&M ScholarWorks: https://scholarworks.wm.edu/vimsarticles/974 NIH Public Access Author Manuscript Comp Biochem Physiol Part D Genomics Proteomics. Author manuscript; available in PMC 2009 December 1. NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author Manuscript Published in final edited form as: Comp Biochem Physiol Part D Genomics Proteomics. 2008 December ; 3(4): 263±269. -
Genome-Wide Phylogenetic Analysis of the Pathogenic Potential of Vibrio
Frontiers in Journal Original Research Date 1 Genome-wide Phylogenetic Analysis of the pathogenic potential of 2 Vibrio furnissii 3 4 Thomas M. Lux 1, Rob Lee 1, John Love 1* 5 1Biosciences, College of Life and Environmental Sciences, The Geoffrey Pope Building, The University of Exeter, Stocker 6 Road, Exeter, EX4 4QD, UK. 7 * Correspondence: John Love, Biosciences, College of Life and Environmental Sciences, The Geoffrey Pope Building, The 8 University of Exeter, Stocker Road, Exeter, EX4 4QD, UK. 9 [email protected] 10 Keywords: Vibrio furnissii, horizontal gene transfer, genome comparison, emerging pathogens, pathogenicity islands, 11 phylogenetic analysis, genome phylogeny. 12 13 Abstract 14 We recently reported the genome sequence of a free-living strain of Vibrio furnissii (NCTC 11218) 15 harvested from an estuarine environment. V. furnissii is a widespread, free-living proteobacterium 16 and emerging pathogen that can cause acute gastroenteritis in humans and lethal zoonoses in aquatic 17 invertebrates, including farmed crustaceans and molluscs. Here we present the analyses to assess the 18 potential pathogenic impact of V. furnissii. We compared the complete genome of V. furnissii with 8 19 other emerging and pathogenic Vibrio species. We selected and analysed more deeply 10 genomic 20 regions based upon unique or common features, and used 3 of these regions to construct a 21 phylogenetic tree. Thus, we positioned V. furnissii more accurately than before and revealed a closer 22 relationship between V. furnissii and V. cholerae than previously thought. However, V. furnissii lacks 23 several important features normally associated with virulence in the human pathogens V. -
Coastal Microbiomes Reveal Associations Between Pathogenic Vibrio Species
1 Coastal microbiomes reveal associations between pathogenic Vibrio species, 2 environmental factors, and planktonic communities 3 Running title: metabarcoding reveals vibrio-plankton associations 4 5 Rachel E. Diner1,2, Drishti Kaul2, Ariel Rabines1,2, Hong Zheng2, Joshua A. Steele3, John F. 6 Griffith3, Andrew E. Allen1,2* 7 8 1 Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 9 92037, USA 10 11 2 Microbial and Environmental Genomics Group, J. Craig Venter Institute, La Jolla, California 12 92037, USA 13 14 3 Southern California Coastal Water Research Project, Costa Mesa, CA 92626, USA 15 16 * Correspondence: [email protected] 17 18 Author Emails: Rachel E. Diner: [email protected], Drishti Kaul: [email protected], Ariel 19 Rabines: [email protected], Hong Zheng: [email protected], Joshua A. Steele: 20 [email protected], John F. Griffith: [email protected], Andrew Allen: [email protected] 21 22 23 1 24 Abstract 25 Background 26 Many species of coastal Vibrio spp. bacteria can infect humans, representing an emerging 27 health threat linked to increasing seawater temperatures. Vibrio interactions with the planktonic 28 community impact coastal ecology and human infection potential. In particular, interactions with 29 eukaryotic and photosynthetic organism may provide attachment substrate and critical nutrients 30 (e.g. chitin, phytoplankton exudates) that facilitate the persistence, diversification, and spread of 31 pathogenic Vibrio spp.. Vibrio interactions with these organisms in an environmental context are, 32 however, poorly understood. 33 34 Results 35 After quantifying pathogenic Vibrio species, including V. cholerae, V. parahaemolyticus, 36 and V. vulnificus, over one year at 5 sites, we found that all three species reached high abundances, 37 particularly during Summer months, and exhibited species-specific temperature and salinity 38 distributions.