Phenotypic and Genotypic Properties of Vibrio Cholerae Non-O1, Non-O139 Isolates Recovered from Domestic Ducks in Germany

Total Page:16

File Type:pdf, Size:1020Kb

Phenotypic and Genotypic Properties of Vibrio Cholerae Non-O1, Non-O139 Isolates Recovered from Domestic Ducks in Germany Characterization of Vibrio spp. isolates from Germany by reference to human clinical isolates using phenotypic and WGS-assisted genotypic methods Inaugural-Dissertation to obtain the academic degree Doctor rerum naturalium (Dr. rer. nat.) submitted to the Department of Biology, Chemistry, Pharmacy of Freie Universität Berlin by Keike Schwartz Berlin 2020 This dissertation was prepared under supervision of Dr. Eckhard Strauch at the Vibrio consiliary laboratory, Department Biological Safety, German Federal Institute for Risk Assessment. May 2016 to December 2020 First examiner: Prof. Dr. Karsten Nöckler Second examiner: Prof. Dr. Jens Rolff Disputation: 05/05/2021 Danksagung An dieser Stelle möchte ich all denjenigen danken, die mich auf meinem Weg zur Promotion begleitet haben. Mein größter Dank gilt Dr. Eckhard Strauch für die Ermöglichung und engagierte Betreuung dieser Doktorarbeit am Konsiliarlabor für Vibrionen sowie seine immerwährende uneingeschränkte Unterstützung. Ganz herzlich bedanke ich mich bei Prof. Dr. Karsten Nöckler und Prof. Dr. Jens Rolff für die Begutachtung dieser Arbeit. Mein besonderer Dank gilt auch Dr. Martin Richter für die Möglichkeit, diese kumulative Dissertation in seiner Fachgruppe anfertigen zu können. Ich danke Dr. Claudia Metelmann und allen Mitarbeitern des IMD Greifswald für die Bereitstellung aktueller klinischer Vibrio-Isolate aus Humanproben sowie Dr. Nadja Bier und Dr. Jens André Hammerl für die bereitwillige Weitergabe wertvoller Erfahrungen zu sequenzbasierten bioinformatischen Analysen. Besonders herzlich möchte ich mich bei Dr. Claudia Jäckel, Cornelia Göllner, Nicole vom Ort und Jonas Nekat aus dem Vibrio-Team für die angenehme, kollegiale Atmosphäre sowie ihre Hilfsbereitschaft und stetige Unterstützung bedanken. In diesem Zusammenhang sei auch allen übrigen Kolleginnen und Kollegen der Fachgruppe 45 für die schöne gemeinsame Zeit gedankt. Mein weiterer Dank gilt Bruno Weser, der stets auf alle großen und kleinen IT-Fragen und -Probleme eine Antwort hatte. Abschließend möchte ich meinen Eltern und meiner Schwester Kerrin danken, die mir in allen Lebenslagen den Rücken gestärkt haben und ohne die ich wahrscheinlich nicht dort stehen würde, wo ich heute bin. III Table of Contents Table of Contents Danksagung............................................................................................................................ III List of Abbreviations .............................................................................................................. VI 1 Introduction ....................................................................................................................... 10 1.1 Vibrio cholerae and Vibrio navarrensis ........................................................................... 10 1.1.1 Taxonomy and Characteristics .............................................................................. 10 1.1.2 Isolation and Identification ................................................................................... 12 1.1.3 Habitats and Ecology ............................................................................................ 13 1.1.4 Virulence Factors .................................................................................................. 17 1.1.5 Human Infections and Infection Epidemiology .................................................... 25 1.2 Aims of the Study ............................................................................................................. 28 2 Publications ........................................................................................................................ 29 2.1 List of Publications and Own Contribution ...................................................................... 29 2.2 Publication 1 ..................................................................................................................... 30 2.3 Publication 2 ..................................................................................................................... 47 3 General Discussion ............................................................................................................ 61 3.1 Background ...................................................................................................................... 61 3.2 Characterization of Environmental Vibrio cholerae Isolates from Germany by Reference to Human Clinical Isolates .............................................................................. 62 3.3 Characterization of Veterinary Vibrio navarrensis Isolates From Germany by Reference to Environmental and Human Clinical Isolates .............................................. 71 3.4 Vibrio spp. Surveillance in Germany and Future Perspectives ........................................ 74 4 Summary ............................................................................................................................ 77 5 Zusammenfassung ............................................................................................................. 79 6 References .......................................................................................................................... 81 IV Table of Contents 7 Appendix .......................................................................................................................... 109 7.1 Supplementary Material ................................................................................................. 109 7.1.1 Publication 1 ....................................................................................................... 109 7.1.2 Publication 2 ....................................................................................................... 131 7.2 Publication 3 ................................................................................................................... 137 7.3 Publications and Congress Presentations ....................................................................... 155 7.4 Curriculum Vitae ............................................................................................................ 157 7.5 Eidesstattliche Erklärung ................................................................................................ 159 V List of Abbreviations List of Abbreviations ACD actin cross-linking domain Acf accessory colonization factor ADP adenosine diphosphate AI-2 autoinducer-2 BON domain bacterial OsmY and nodulation domain bp base pair(s) BPI bactericidal/permeability-increasing protein BWM Ballast Water Management Caco-2 human epithelial colon adenocarcinoma cell line CAI-1 cholera autoinducer-1 cAMP cyclic adenosine monophosphate CDC Centers for Disease Control and Prevention CFU colony forming unit(s) Changliver HeLa derivative ChxA cholix toxin cps gene encoding a putative capsule biosynthesis protein CqsA CAI-1 autoinducer synthase CqsS CAI-1 autoinducer receptor CTX cholera toxin ctxA gene encoding the A subunit of the cholera toxin CTXΦ lysogenic filamentous bacteriophage whose genome encodes the cholera toxin dctM, dctP, dctQ genes encoding proteins involved in sialic acid transport δ-VPH delta V. parahaemolyticus hemolysin DLV double-locus variant DNA deoxyribonucleic acid DncV V. cholerae dinucleotide cyclase DT diphtheria toxin DTH delta thermostable hemolysin ECDC European Centre for Disease Prevention and Control eEF eukaryotic elongation factor VI List of Abbreviations HA/P; HapA hemagglutinin/protease Hcp hemolysin-coregulated protein HeLa human epithelial cervix adenocarcinoma cell line HHM Hafen Hamburg Marketing e.V. HisF protein of the baseplate structure of the V. cholerae T6SS HlyA hemolysin A HlyAET El Tor variant of hemolysin A HlyD hemolysin D protein HlyIII hemolysin III protein IfSG Infektionsschutzgesetz IMO International Maritime Organization ISO International Organization for Standardization kb kilobase(s) kbp kilobase pair(s) KLIWAS Auswirkungen des Klimawandels auf Wasserstraßen und Schifffahrt in Deutschland (research program) LDH lecithin-dependent hemolysin LuxPQ AI-2 autoinducer receptor LuxS AI-2 autoinducer synthase MARTX multifunctional-autoprocessing repeats-in-toxin toxin Mb megabase(s) MDa megadalton μm micrometer MLSA multilocus sequence analysis MLST multilocus sequence typing MPN most probable number MSHA mannose-sensitive hemagglutinin NaCl sodium chloride nagA, nanA, nanE, nanK genes necessary for sialic acid catabolism nanH gene necessary for sialic acid scavenging Neu5Ac N-acetylneuraminic acid O-antigen surface antigen O1 O1-antigen O139 O139-antigen VII List of Abbreviations OmpT outer membrane protein T OmpU outer membrane protein U ORF open reading frame OsmY putative BON domain-containing hemolysin in V. navarrensis PAAR-motif proteins proline-alanine-alanine-arginine motif proteins PCR polymerase chain reaction pilV, pilW genes encoding proteins for type IV pilus biosynthesis ppt parts per thousand pVH plasmid VH QMRA quantitative microbial risk assessment RadC DNA repair protein RAW 264.7 murine macrophage cell line rfb O1 O1-antigen-specific genomic region rfb O139 O139-antigen-specific genomic region RNA ribonucleic acid RNase H1 ribonuclease H1 rpoB gene encoding the β subunit of RNA polymerase RstA protein required for replication of CTXΦ RstB protein required for site-specific integration of CTXΦ RstR repressor of rstA and rstB transcription rtx repeats-in-toxin gene SA sialic acid SLV single-locus variant SNP single nucleotide polymorphism SST sea surface temperature ST sequence type
Recommended publications
  • Chitin Metabolism by Vibrio Furnissii: Quantification of Nage Expression
    Chitin Metabolism by Vibrio furnissii: Quantification of nagE Expression by Sarah G. Brown A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree Master of Science Major Subject: Interdisciplinary Sciences West Texas A&M University Canyon, Texas May 2017 Abstract The phosphoenolpyruvate: sugar phosphotransferase system (PTS) was first discovered in the 1960s by Kundig et al. The PTS is unique to bacteria, and is a rich area of study offering an abundance of potential research topics due to its environmental role and its potential as a target for future antibiotics. This study focuses on the nag operon, which plays an important role in chitin degradation. The expression of nagE, one gene located on the nag operon, was assessed via quantitative PCR (qPCR) in the presence of four substrates. This gene encodes the N-acetylglucosamine transporter protein. Expression of the gene was found to be up-regulated in the presence of N-acetylglucosamine, but not in the presence of glucose, mannose, or lactate. Potential future projects include: the quantification of expression of nagA via qPCR; the use of a reporter gene to quantify expression of nagE and nagA; study of NagC, thought to be the repressor of the nag operon; and further study and characterization of the gene encoding for the glucose specific transporter protein in V. furnissii. ii Acknowledgements I would like to thank Dr. Carolyn Bouma, first and foremost, for her invaluable guidance and expertise, as well as her time, patience, and encouragement during the course of this project. I would also like to thank Dr. Donna Byers, for time spent instructing me on how to carry out a gene expression study using qPCR, as well as the use of her Nanodrop system; Dr.
    [Show full text]
  • Vibrios As Causal Agents of Zoonoses B
    Vibrios as causal agents of zoonoses B. Austin To cite this version: B. Austin. Vibrios as causal agents of zoonoses. Veterinary Microbiology, Elsevier, 2010, 140 (3-4), pp.310. 10.1016/j.vetmic.2009.03.015. hal-00556049 HAL Id: hal-00556049 https://hal.archives-ouvertes.fr/hal-00556049 Submitted on 15 Jan 2011 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Accepted Manuscript Title: Vibrios as causal agents of zoonoses Author: B. Austin PII: S0378-1135(09)00119-9 DOI: doi:10.1016/j.vetmic.2009.03.015 Reference: VETMIC 4385 To appear in: VETMIC Received date: 9-1-2009 Revised date: 9-2-2009 Accepted date: 2-3-2009 Please cite this article as: Austin, B., Vibrios as causal agents of zoonoses, Veterinary Microbiology (2008), doi:10.1016/j.vetmic.2009.03.015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.
    [Show full text]
  • Genome-Wide Phylogenetic Analysis of the Pathogenic Potential of Vibrio
    Frontiers in Journal Original Research Date 1 Genome-wide Phylogenetic Analysis of the pathogenic potential of 2 Vibrio furnissii 3 4 Thomas M. Lux 1, Rob Lee 1, John Love 1* 5 1Biosciences, College of Life and Environmental Sciences, The Geoffrey Pope Building, The University of Exeter, Stocker 6 Road, Exeter, EX4 4QD, UK. 7 * Correspondence: John Love, Biosciences, College of Life and Environmental Sciences, The Geoffrey Pope Building, The 8 University of Exeter, Stocker Road, Exeter, EX4 4QD, UK. 9 [email protected] 10 Keywords: Vibrio furnissii, horizontal gene transfer, genome comparison, emerging pathogens, pathogenicity islands, 11 phylogenetic analysis, genome phylogeny. 12 13 Abstract 14 We recently reported the genome sequence of a free-living strain of Vibrio furnissii (NCTC 11218) 15 harvested from an estuarine environment. V. furnissii is a widespread, free-living proteobacterium 16 and emerging pathogen that can cause acute gastroenteritis in humans and lethal zoonoses in aquatic 17 invertebrates, including farmed crustaceans and molluscs. Here we present the analyses to assess the 18 potential pathogenic impact of V. furnissii. We compared the complete genome of V. furnissii with 8 19 other emerging and pathogenic Vibrio species. We selected and analysed more deeply 10 genomic 20 regions based upon unique or common features, and used 3 of these regions to construct a 21 phylogenetic tree. Thus, we positioned V. furnissii more accurately than before and revealed a closer 22 relationship between V. furnissii and V. cholerae than previously thought. However, V. furnissii lacks 23 several important features normally associated with virulence in the human pathogens V.
    [Show full text]
  • Coastal Microbiomes Reveal Associations Between Pathogenic Vibrio Species
    1 Coastal microbiomes reveal associations between pathogenic Vibrio species, 2 environmental factors, and planktonic communities 3 Running title: metabarcoding reveals vibrio-plankton associations 4 5 Rachel E. Diner1,2, Drishti Kaul2, Ariel Rabines1,2, Hong Zheng2, Joshua A. Steele3, John F. 6 Griffith3, Andrew E. Allen1,2* 7 8 1 Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 9 92037, USA 10 11 2 Microbial and Environmental Genomics Group, J. Craig Venter Institute, La Jolla, California 12 92037, USA 13 14 3 Southern California Coastal Water Research Project, Costa Mesa, CA 92626, USA 15 16 * Correspondence: [email protected] 17 18 Author Emails: Rachel E. Diner: [email protected], Drishti Kaul: [email protected], Ariel 19 Rabines: [email protected], Hong Zheng: [email protected], Joshua A. Steele: 20 [email protected], John F. Griffith: [email protected], Andrew Allen: [email protected] 21 22 23 1 24 Abstract 25 Background 26 Many species of coastal Vibrio spp. bacteria can infect humans, representing an emerging 27 health threat linked to increasing seawater temperatures. Vibrio interactions with the planktonic 28 community impact coastal ecology and human infection potential. In particular, interactions with 29 eukaryotic and photosynthetic organism may provide attachment substrate and critical nutrients 30 (e.g. chitin, phytoplankton exudates) that facilitate the persistence, diversification, and spread of 31 pathogenic Vibrio spp.. Vibrio interactions with these organisms in an environmental context are, 32 however, poorly understood. 33 34 Results 35 After quantifying pathogenic Vibrio species, including V. cholerae, V. parahaemolyticus, 36 and V. vulnificus, over one year at 5 sites, we found that all three species reached high abundances, 37 particularly during Summer months, and exhibited species-specific temperature and salinity 38 distributions.
    [Show full text]
  • Vibrio Aerogenes Sp. Nov., a Facultatively Anaerobic Marine Bacterium That Ferments Glucose with Gas Production
    International Journal of Systematic and Evolutionary Microbiology (2000), 50, 321–329 Printed in Great Britain Vibrio aerogenes sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production Wung Yang Shieh, Aeen-Lin Chen† and Hsiu-Hui Chiu Author for correspondence: Wung Yang Shieh. Tel. ­886 2 23636040 ext. 417 Fax: ­886 2 23626092. e-mail: winyang!ms.cc.ntu.edu.tw Institute of Oceanography, A mesophilic, facultatively anaerobic, marine bacterium, designated strain National Taiwan FG1T, was isolated from a seagrass bed sediment sample collected from University, PO Box 23-13, Taipei, Taiwan Nanwan Bay, Kenting National Park, Taiwan. Cells grown in broth cultures were motile, Gram-negative rods; motility was normally achieved by two sheathed flagella at one pole of the cell. Strain FG1T required NaM for growth, and exhibited optimal growth at 30–35 SC, pH 6–7 and about 4% NaCl. It grew anaerobically by fermenting glucose and other carbohydrates with production of various organic acids, including acetate, lactate, formate, malate, oxaloacetate, propionate, pyruvate and succinate, and the gases CO2 and H2. The strain did not require either vitamins or other organic growth factors for growth. Its DNA GMC content was 45<9 mol%. It contained C12:0 as the most abundant cellular fatty acid. Characterization data, together with the results of a 16S rDNA-based phylogenetic analysis, indicate that strain FG1T represents a new species of the genus Vibrio. Thus, the name Vibrio aerogenes sp. nov. is proposed for this new bacterium. The type strain is FG1T (¯ ATCC 700797T ¯ CCRC 17041T). Keywords: Vibrio aerogenes sp.
    [Show full text]
  • Vibrio Furnissii (Formerly Aerogenic Biogroup of Vibrio Fluvialis), a New Species Isolated from Human Feces and the Environment DON J
    JOURNAL OF CLINICAL MICROBIOLOGY, OCt. 1983, p. 816-824 Vol. 18, No. 4 0095-1137/83/100816-09$02.00/0 Vibrio furnissii (Formerly Aerogenic Biogroup of Vibrio fluvialis), a New Species Isolated from Human Feces and the Environment DON J. BRENNER,'* FRANCES W. HICKMAN-BRENNER,2 JOHN V. LEE,3 ARNOLD G. STEIGERWALT,1 G. RICHARD FANNING,4 DANNIE G. HOLLIS,5 J. J. FARMER III,2 ROBERT E. WEAVER,5 S. W. JOSEPH,6 AND RAMON J. SEIDLER7 Molecular Biology Laboratory,1 Enteric Bacteriology Section,2 and Special Bacterial Reference Activity,5 Division ofBacterial Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333; Environmental Microbiology and Safety Reference Laboratory, Public Health Laboratory Service, Center for Applied Microbiology and Research, Porton Down, Salisbury SP4 OJG, United Kingdom3; Division ofBiochemistry, Walter Reed Army Institute ofResearch, Washington, D.C. 200124; Department of Microbiology, University of Maryland, College Park, Maryland 207426; and Department of Microbiology, Oregon State University, Corvallis, Oregon 973317 Received 7 April 1983/Accepted 13 July 1983 Strains formerly classified as the aerogenic (gas-producing) biogroup of Vibrio fluvialis were shown by DNA relatedness to be a separate species. The species was named Vibrio furnissii sp. nov. (type strain ATCC 35016 =CDC B3215). Three strains of V. furnissii were 79% or more related to the type strain of V. furnissii and about 50% related to the type strain of V. fluvialis. V. fluvialis strains were 40 to 64% related to the type strain of V. furnissii. Divergence in related sequences was only 0.0 to 1.5% among strains of V.
    [Show full text]
  • Sequence Characterization and Comparative Analysis of Three Plasmids Isolated from Environmental Vibrio Spp.ᰔ† Tracy H
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 2007, p. 7703–7710 Vol. 73, No. 23 0099-2240/07/$08.00ϩ0 doi:10.1128/AEM.01577-07 Copyright © 2007, American Society for Microbiology. All Rights Reserved. Sequence Characterization and Comparative Analysis of Three Plasmids Isolated from Environmental Vibrio spp.ᰔ† Tracy H. Hazen,1 Dongying Wu,2,3 Jonathan A. Eisen,2,3 and Patricia A. Sobecky1* School of Biology, Georgia Institute of Technology, Atlanta, Georgia 303321; The Institute for Genomic Research, Rockville, Maryland 208502; and UC Davis Genome Center, University of California, Davis, Davis, California 956163 Received 11 July 2007/Accepted 26 September 2007 The horizontal transfer of genes by mobile genetic elements such as plasmids and phages can accelerate genome diversification of Vibrio spp., affecting their physiology, pathogenicity, and ecological character. In this study, sequence analysis of three plasmids from Vibrio spp. previously isolated from salt marsh sediment revealed the remarkable diversity of these elements. Plasmids p0908 (81.4 kb), p23023 (52.5 kb), and p09022 kb) had a predicted 99, 64, and 32 protein-coding sequences and G؉C contents of 49.2%, 44.7%, and 31.0) 42.4%, respectively. A phylogenetic tree based on concatenation of the host 16S rRNA and rpoA nucleotide sequences indicated p23023 and p09022 were isolated from strains most closely related to V. mediterranei and V. campbellii, respectively, while the host of p0908 forms a clade with V. fluvialis and V. furnissii. Many predicted proteins had amino acid identities to proteins of previously characterized phages and plasmids (24 to 94%). Predicted proteins with similarity to chromosomally encoded proteins included RecA, a nucleoid-associated protein (NdpA), a type IV helicase (UvrD), and multiple hypothetical proteins.
    [Show full text]
  • The Incidence of Vibrio Species in Louisiana and Maryland Oysters (Crassostrea Virginica)
    Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1990 The ncI idence of Vibrio Species in Louisiana and Maryland Oysters (Crassostrea Virginica). Mary Lynelle Ford Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Ford, Mary Lynelle, "The ncI idence of Vibrio Species in Louisiana and Maryland Oysters (Crassostrea Virginica)." (1990). LSU Historical Dissertations and Theses. 4914. https://digitalcommons.lsu.edu/gradschool_disstheses/4914 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS The most advanced technology has been used to photograph and reproduce this manuscript from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps.
    [Show full text]
  • Vibrio Fluvialis: an Emerging Human Pathogen
    REVIEW ARTICLE published: 07 March 2014 doi: 10.3389/fmicb.2014.00091 Vibrio fluvialis: an emerging human pathogen Thandavarayan Ramamurthy 1*, Goutam Chowdhury 1, Gururaja P.Pazhani 1 and Sumio Shinoda 2 1 National Institute of Cholera and Enteric Diseases, Kolkata, India 2 National Institute of Cholera and Enteric Diseases, Collaborative Research Center of Okayama University for Infectious Diseases in India, Kolkata, India Edited by: Vibrio fluvialis is a pathogen commonly found in coastal environs. Considering recent Rita R. Colwell, University of increase in numbers of diarrheal outbreaks and sporadic extraintestinal cases, V.fluvialis has Maryland, USA been considered as an emerging pathogen. Though this pathogen can be easily isolated Reviewed by: by existing culture methods, its identification is still a challenging problem due to close Carlos R. Osorio, University of Santiago de Compostela, Spain phenotypic resemblance either with Vibrio cholerae or Aeromonas spp. However, using Brian Austin, University of Stirling, UK molecular tools, it is easy to identify V. fluvialis from clinical and different environmental *Correspondence: samples. Many putative virulence factors have been reported, but its mechanisms of Thandavarayan Ramamurthy, National pathogenesis and survival fitness in the environment are yet to be explored. This chapter Institute of Cholera and Enteric covers some of the major discoveries that have been made to understand the importance Diseases, P-33, CIT Road, Scheme-XM, Beliaghata, of V. fluvialis. Kolkata-700010, India Keywords:V. fluvialis, diarrhea, virulence factors, antimicrobial resistance, molecular typing e-mail: [email protected] INTRODUCTION importance of V. fluvialis (Chowdhury et al., 2012; Liang et al., Vibrio fluvialis is a halophilic Gram-negative bacterium, which 2013).
    [Show full text]
  • Deep-Sea Hydrothermal Vent Bacteria Related to Human Pathogenic Vibrio
    Deep-sea hydrothermal vent bacteria related to human PNAS PLUS pathogenic Vibrio species Nur A. Hasana,b,c, Christopher J. Grima,c,1, Erin K. Lippd, Irma N. G. Riverae, Jongsik Chunf, Bradd J. Haleya, Elisa Taviania, Seon Young Choia,b, Mozammel Hoqg, A. Christine Munkh, Thomas S. Brettinh, David Bruceh, Jean F. Challacombeh, J. Chris Detterh, Cliff S. Hanh, Jonathan A. Eiseni, Anwar Huqa,j, and Rita R. Colwella,b,c,k,2 aMaryland Pathogen Research Institute, cUniversity of Maryland Institute for Advanced Computer Studies, and jInstitute for Applied Environmental Health, University of Maryland, College Park, MD 20742; bCosmosID, College Park, MD 20742; dEnvironmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602; eDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, CEP 05508-900 São Paulo, Brazil; fSchool of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea; gDepartment of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh; hGenome Science Group, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545; iUniversity of California Davis Genome Center, Davis, CA 95616; and kBloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21205 Contributed by Rita R. Colwell, April 15, 2015 (sent for review September 5, 2014; reviewed by John Allen Baross, Richard E. Lenski, and Carla Pruzzo) Vibrio species are both ubiquitous and abundant in marine coastal of vibrios, and suggested Vibrio populations generally comprise waters, estuaries, ocean sediment, and aquaculture settings world- approximately 1% (by molecular techniques) of the total bac- wide. We report here the isolation, characterization, and genome terioplankton in estuaries (19), in contrast to culture-based studies sequence of a novel Vibrio species, Vibrio antiquarius, isolated from demonstrating that vibrios can comprise up to 10% of culturable a mesophilic bacterial community associated with hydrothermal marine bacteria (20).
    [Show full text]
  • Species of the Genus Vibrio of Clinical–Epidemiological Importance ©2021 Monsreal Et Al
    MOJ Biology and Medicine Research Article Open Access Species of the genus Vibrio of clinical– epidemiological importance Abstract Volume 9 Issue 3 - 2021 Vibrios are characteristically indigenous to marine, brackish, and estuarine habitats, and José Franco Monsreal,1 Lidia Esther del appear in large concentrations (blooms) when water temperatures rise (17–20°C). At low Socorro Serralta–Peraza,1 Javier Jesús Flores temperatures, the vibrios remain in the sediment of the seabed and the counts are usually 2 lower than those necessary to cause infection. In temperate countries, vibrios are present Abuxapqui 1Department of Health and Community Development, in seawater throughout the year, although their concentration undergoes a notable increase University of Quintana Roo, Mexico in the warm months due to favorable ecological conditions and plankton, increasing their 2Department of Microbiology, University of Quintana Roo accumulation by filter feeders and other marine animals. The objective of the present study Mexico was to determine whether raw seafood, marinated without heat, partially cooked with heat, and completely cooked with heat represent potential risk factors for the species Vibrio Correspondence: José Franco Monsreal, Departamento de damsela, Vibrio fluvialis and Vibrio furnissii for the development, respectively, of infection. Salud y Desarrollo Comunitario. Universidad Intercultural wound; of acute gastroenteritis; and acute gastroenteritis. A list of establishments specialized Maya de Quintana Roo. Carretera Muna–Felipe Carrillo Puerto in the sale of seafood for human consumption was obtained. The amount of seafood in S/N, km. 137. CP. 77870. La, Mexico, Tel 999 136 5329, ORCID these establishments was 390. For the homogenization and enrichment of each sample, as 0000–0002–4019–7640, Email well as for the isolation and identification of the three species, we proceeded according to the methodology described in the eighth edition of the Bacteriological Analytical Manual Received: July 03, 2021 | Published: July 15, 2021 (FDA).
    [Show full text]
  • Id 19
    UK Standards for Microbiology Investigations 2014 Identification of Vibrio species FEBRUARY 24 - JANUARY 24 BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 19 | Issue no: di+ | Issue date: dd.mm.yy <tab+enter> | Page: 1 of 23 © Crown copyright 2013 Identification of Vibrio species Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website http://www.hpa.org.uk/SMI/Partnerships. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see http://www.hpa.org.uk/SMI/WorkingGroups). The contributions of many individuals in clinical, specialist and reference laboratories2014 who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: FEBRUARY 24 Standards Unit - Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ JANUARY E-mail: [email protected] 24 Website: http://www.hpa.org.uk/SMI UK Standards for Microbiology Investigations are produced in association with: BETWEEN ON CONSULTED WAS DOCUMENT THIS - DRAFT Bacteriology – Identification | ID 19 | Issue no: di+ | Issue date: dd.mm.yy <tab+enter> | Page: 2 of 23 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Vibrio species Contents ACKNOWLEDGMENTS .......................................................................................................... 2 AMENDMENT TABLE ............................................................................................................
    [Show full text]