MCM2 Mouse Monoclonal Antibody [Clone ID: OTI1G4] Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA805472

MCM2 Mouse Monoclonal Antibody [Clone ID: OTI1G4] Product data:

Product Type: Primary Antibodies Clone Name: OTI1G4 Applications: IHC, WB Recommended Dilution: WB 1:2000, IHC 1:150 Reactivity: Human, Mouse, Rat Host: Mouse Isotype: IgG2b Clonality: Monoclonal Immunogen: Full length human recombinant protein of human MCM2 (NP_004517) produced in HEK293T cell. Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Concentration: 1 mg/ml Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Predicted Protein Size: 101.7 kDa Gene Name: minichromosome maintenance complex component 2 Database Link: NP_004517 Entrez Gene 17216 MouseEntrez Gene 312538 RatEntrez Gene 4171 Human P49736

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 4 MCM2 Mouse Monoclonal Antibody [Clone ID: OTI1G4] – TA805472

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors Protein Pathways: Cell cycle, DNA replication

Product images:

HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY MCM2 ([RC202506], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2. Positive lysates [LY401445] (100ug) and [LC401445] (20ug) can be purchased separately from OriGene.

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 4 MCM2 Mouse Monoclonal Antibody [Clone ID: OTI1G4] – TA805472

Immunohistochemical staining of paraffin- embedded Human colon tissue within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805472)

Immunohistochemical staining of paraffin- embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805472)

Immunohistochemical staining of paraffin- embedded Carcinoma of Human lung tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805472)

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 4 MCM2 Mouse Monoclonal Antibody [Clone ID: OTI1G4] – TA805472

Immunohistochemical staining of paraffin- embedded Human tonsil within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805472)