DOCSLIB.ORG

Oxyurida: Oxyuridae

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Oxyurida: Oxyuridae Cao et al. Parasites Vectors (2020) 13:566 https://doi.org/10.1186/s13071-020-04430-6 Parasites & Vectors RESEARCH Open Access Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) Yi‑Fan Cao1,2†, Hui‑Xia Chen3†, Yang Li3, Dang‑Wei Zhou1,4*, Shi‑Long Chen1,4 and Liang Li3* Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai‑Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the para‑ sites of the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochon‑ drial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplifed and sequenced for molecular identifcation. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S 28S cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. + + Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecifc nucleotide variation between diferent individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the *Correspondence: [email protected]; [email protected] Background †Yi‑Fan Cao and Hui‑Xia Chen contributed equally to this work Te Tibetan antelope Pantholops hodgsonii (Abel) 1 Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, (Artiodactyla: Bovidae) is an endangered species of Xining 810008, Qinghai, People’s Republic of China mammal endemic to the Qinghai-Tibetan Plateau. Te 3 Key Laboratory of Animal Physiology, Biochemistry and Molecular population of the Tibetan antelope has been declined Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei Province, People’s Republic severely, because of the loss and fragmentation of its of China habitat and commercial poaching [1, 2]. Te latest esti- Full list of author information is available at the end of the article mate of the global population of the Tibetan antelope is © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/publi cdoma in/ zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Cao et al. Parasites Vectors (2020) 13:566 Page 2 of 11 subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the tradi‑ tional classifcation, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skr- jabinema longicaudatum n. sp. represents the frst species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema. Keywords: Tibetan antelope, Parasite, Nematoda, Morphology, Genetic data, Phylogeny 100,000–150,000 mature individuals (https ://www.iucnr Morphological observations edlis t.org/speci es/15967 /50192 544). Tis species is listed For light microscopical studies, nematodes were cleared as “Near Treatened” in the IUCN Red List of Treatened in lactophenol. Drawings were made using a Nikon Species™ and also listed as Class I (Endangered in China) microscope drawing attachment. For scanning electron National Protected Wild Animal Species in China. microscopy (SEM), the anterior and posterior parts of Parasites and parasitic diseases are considered to be specimens were re-fxed in 4% formaldehyde solution, important threats in wildlife conservation, as they can post-fxed in 1% OsO4, dehydrated via an ethanol series potentially impair the health of wildlife, decrease fit- and acetone, and then critical-point dried. Samples were ness, cause population declines and even contribute coated with gold and examined using a Hitachi S-4800 to local extinction [3–7]. Parasites are also significant scanning electron microscope at an accelerating voltage pathogens of the Tibetan antelope [1, 2]. To date, 17 of 20 kV. Measurements (the range, followed by the mean species of ectoparasites and endoparasites have been values in parentheses) are given in micrometers (μm) reported from the Tibetan antelope, including 5 spe- unless otherwise stated. cies of oestrid and hippoboscid flies, 7 species of protozoans, 2 species of tapeworms and 3 species of Molecular protocols nematodes [1, 2, 8–12]. Tree female specimens were randomly chosen for In the present study, some nematode specimens molecular analysis. Genomic DNA from each sample were collected from the digestive tract of the Tibetan was extracted using a Column Genomic DNA Isolation antelope, which were identifed morphologically as Kit (Shanghai Sangon, China) according to the manufac- a new species of the genus Skrjabinema (Oxyurida: turer’s instructions. Te partial 18S region was amplifed Oxyuridae) using light and scanning electron micros- by polymerase chain reaction (PCR) using the forward copy. Te nuclear and mitochondrial DNA sequences, primer 18S-F (5’-CGC GAA TRG CTC ATT ACA ACA i.e. small subunit ribosomal DNA (18S), large subunit GC-3’) and the reverse primer 18S-R (5’-GGG CGG ribosomal DNA (28S), internal transcribed spacer (ITS) TAT CTG ATC GCC-3’) [13]. Te partial 28S region was and cytochrome c oxidase subunit 1 (cox1), were also amplifed by PCR using the forward primer 28S-F (5’- amplifed and sequenced for molecular identifcation of AGC GGA GGA AAA GAA ACT AA-3’) and the reverse this species. Moreover, in order to clarify the system- primer 28S-R (5’-ATC CGT GTT TCA AGA CGG G-3’) atic status of the genus Skrjabinema, phylogenetic anal- [14]. Te ITS-1 region of nuclear rDNA was amplifed by yses were performed using maximum likelihood (ML) PCR using the forward primer SS1 (5’-GTT TCC GTA inference based on 28S and 18S + 28S + cox1 sequence GGT GAA CCT GCG-3’) and the reverse primer SS2R data, respectively. (5’-AGT GCT CAA TGT GTC TGC AA-3’). Te ITS-2 region of nuclear rDNA was amplifed by PCR using the Methods forward primer NC13 (5’-ATC GAT GAA GAA CGC Parasite collection AGC-3’) and the reverse primer NC2 (reverse: 5’-TTA A Tibetan antelope died naturally in the Hohxil National GTT TCT TTT CCT CCG CT-3’) [15]. Te partial cox1 Nature Reserve, Qinghai Province, China. Te digestive region was amplifed by PCR using the forward primer tract of this Tibetan antelope was sent to the Key Lab- cox1F2020 (5’-GAG TAC TAA TCA TAA GGA TAT oratory of Adaptation and Evolution of Plateau Biota TGG-3’) and the reverse primer cox1R2020 (5’-ACA TAA (AEPB), Northwest Institute of Plateau Biology, Chinese ACY TCA GGA TGA CCA-3’), both newly designed in Academy of Sciences for examination of parasites. Large present study. Te cycling conditions are as described numbers of nematode parasites were isolated from the previously [16]. PCR products were checked on Gold- caecum and colon. Specimens were fxed and stored in in View-stained 1.5% agarose gels and purifed with Column 5% glycerine plus 70% ethanol until study. PCR Product Purifcation Kit (Shanghai Sangon, China). Cao et al. Parasites Vectors (2020) 13:566 Page 3 of 11 Sequencing was carried out using a Dye Deoxy Termina- Evolution of Plateau Biota, Northwest Institute of Plateau tor Cycle Sequencing Kit (v.2, Applied Biosystems, Cali- Biology, Chinese Academy of Sciences, Qinghai Province, fornia, USA) and an automated sequencer (ABI-PRISM China. 377). Sequences were aligned using ClustalW2. Te DNA sequences obtained herein were compared (using the Site in host algorithm BLASTn) with those available in the National Caecum and colon. Center for Biotechnology Information (NCBI) database (https ://www.ncbi.nlm.nih.gov). Prevalence and intensity A single Tibetan antelope examined with 124 worms. Phylogenetic analyses Phylogenetic trees were constructed using maximum ZooBank registration likelihood (ML) inference with MEGA X software based To comply with the regulations set out in Arti- on the partial 28S and 18S + 28S + cox1 sequence data, cle 8.5 of the amended 2012 version of the Interna- respectively. Pseudonymus spirotheca (Oxyurida: Te- tional Code of Zoological Nomenclature (ICZN) [17], lastomatoidea: Pseudonymidae) was treated as the out- details of the new species have been submitted to group.
Load more

Recommended publications
  • Redalyc.New Species of Aspiculuris (Nematoda: Heteroxynematidae
    Revista Mexicana de Biodiversidad ISSN: 1870-3453 [email protected] Universidad Nacional Autónoma de México México Falcón-Ordaz, Jorge; Pulido-Flores, Griselda; Monks, Scott New species of Aspiculuris (Nematoda: Heteroxynematidae), parasite of Mus musculus (Rodentia: Muridae), from Hidalgo, Mexico Revista Mexicana de Biodiversidad, vol. 81, núm. 3, 2010, pp. 669-676 Universidad Nacional Autónoma de México Distrito Federal, México Available in: http://www.redalyc.org/articulo.oa?id=42518439008 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Revista Mexicana de Biodiversidad 81: 669 - 676, 2010 New species of Aspiculuris (Nematoda: Heteroxynematidae), parasite of Mus musculus (Rodentia: Muridae), from Hidalgo, Mexico Una nueva especie de Aspiculuris (Nematoda: Heteroxynematidae), parásito de Mus musculus (Rodentia: Muridae), de Hidalgo, México Jorge Falcón-Ordaz, Griselda Pulido-Flores and Scott Monks* Universidad Autónoma del Estado de Hidalgo, Centro de Investigaciones Biológicas, Apartado postal 1-69, 42001 Pachuca, Hidalgo, México. *Correspondent: [email protected] Abstract. Aspiculuris huascaensis n. sp. was found in the intestine of Mus musculus collected from 2 localities in Hidalgo, Mexico, and is described herein. The new species possesses cervical alae abruptly interrupted at mid-length of esophageal bulb form an acute angle, distinguishing it from 5 of the 17 species in the genus. The new species is differentiated from 11 of the remaining species by having cervical alae that form an acute angle and end at mid-length of the esophageal bulb.
    [Show full text]
  • Gastrointestinal Helminthic Parasites of Habituated Wild Chimpanzees
    Aus dem Institut für Parasitologie und Tropenveterinärmedizin des Fachbereichs Veterinärmedizin der Freien Universität Berlin Gastrointestinal helminthic parasites of habituated wild chimpanzees (Pan troglodytes verus) in the Taï NP, Côte d’Ivoire − including characterization of cultured helminth developmental stages using genetic markers Inaugural-Dissertation zur Erlangung des Grades eines Doktors der Veterinärmedizin an der Freien Universität Berlin vorgelegt von Sonja Metzger Tierärztin aus München Berlin 2014 Journal-Nr.: 3727 Gedruckt mit Genehmigung des Fachbereichs Veterinärmedizin der Freien Universität Berlin Dekan: Univ.-Prof. Dr. Jürgen Zentek Erster Gutachter: Univ.-Prof. Dr. Georg von Samson-Himmelstjerna Zweiter Gutachter: Univ.-Prof. Dr. Heribert Hofer Dritter Gutachter: Univ.-Prof. Dr. Achim Gruber Deskriptoren (nach CAB-Thesaurus): chimpanzees, helminths, host parasite relationships, fecal examination, characterization, developmental stages, ribosomal RNA, mitochondrial DNA Tag der Promotion: 10.06.2015 Contents I INTRODUCTION ---------------------------------------------------- 1- 4 I.1 Background 1- 3 I.2 Study objectives 4 II LITERATURE OVERVIEW --------------------------------------- 5- 37 II.1 Taï National Park 5- 7 II.1.1 Location and climate 5- 6 II.1.2 Vegetation and fauna 6 II.1.3 Human pressure and impact on the park 7 II.2 Chimpanzees 7- 12 II.2.1 Status 7 II.2.2 Group sizes and composition 7- 9 II.2.3 Territories and ranging behavior 9 II.2.4 Diet and hunting behavior 9- 10 II.2.5 Contact with humans 10 II.2.6
    [Show full text]
  • Cestoda: Anoplocephalidae)
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Dissertations and Theses in Biological Sciences Biological Sciences, School of 5-2009 Taxonomic Revision of Species of the Genus Monoecocestus (Cestoda: Anoplocephalidae) Terry R. Haverkost University of Nebraska - Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/bioscidiss Part of the Parasitology Commons, and the Zoology Commons Haverkost, Terry R., "Taxonomic Revision of Species of the Genus Monoecocestus (Cestoda: Anoplocephalidae)" (2009). Dissertations and Theses in Biological Sciences. 56. https://digitalcommons.unl.edu/bioscidiss/56 This Article is brought to you for free and open access by the Biological Sciences, School of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Dissertations and Theses in Biological Sciences by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. TAXONOMIC REVISION OF SPECIES OF THE GENUS MONOECOCESTUS (CESTODA: ANOPLOCEPHALIDAE) By Terry R. Haverkost A DISSERTATION Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Doctor of Philosophy Major: Biological Sciences Under the Supervision of Professor Scott L. Gardner Lincoln, Nebraska May, 2009 TAXONOMIC REVISION OF SPECIES OF THE GENUS MONOECOCESTUS (CESTODA: ANOPLOCEPHALIDAE) Terry R. Haverkost University of Nebraska, 2009 Advisor: Scott L. Gardner My dissertation research is an important contribution to the taxonomy of anoplocephalid cestodes. Almost all research conducted for these chapters was done by staining, mounting, and measuring anoplocephalid cestodes from the Bolivian Biodiversity Survey conducted in Bolivia from 1984-2000. These specimens were collected and processed in the field and deposited in the Harold W.
    [Show full text]
  • Fibre Couplings in the Placenta of Sperm Whales, Grows to A
    news and views Most (but not all) nematodes are small Daedalus and nondescript. For example, Placento- T STUDIOS nema gigantissima, which lives as a parasite Fibre couplings in the placenta of sperm whales, grows to a CS./HOL length of 8 m, with a diameter of 2.5 cm. The The nail, says Daedalus, is a brilliant and free-living, marine Draconema has elongate versatile fastener, but with a fundamental O ASSO T adhesive organs on the head and along the contradiction. While being hammered in, HO tail, and moves like a caterpillar. But the gen- it is a strut, loaded in compression. It must BIOP eral uniformity of most nematode species be thick enough to resist buckling. Yet has hampered the establishment of a classifi- once in place it is a tie, loaded in tension, 8 cation that includes both free-living and par- and should be thin and flexible to bear its asitic species. Two classes have been recog- load efficiently. He is now resolving this nized (the Secernentea and Adenophorea), contradiction. based on the presence or absence of a caudal An ideal nail, he says, should be driven sense organ, respectively. But Blaxter et al.1 Figure 2 The bad — eelworm (root knot in by a force applied, not to its head, but to have concluded from the DNA sequences nematode), which forms characteristic nodules its point. Its shaft would then be drawn in that the Secernentea is a natural group within on the roots of sugar beet and rice. under tension; it could not buckle, and the Adenophorea.
    [Show full text]
  • Journal of the Helminthological Society of Washington 63(2) 1996
    July 1996 Number 2 Of of Washington A semiannual journal of research devoted to Helminthology and all branches of Parasitology Supported in part by the Brayton H. Ransom Memorial Trust Fund D. C. KRITSKY, W. A. :B6EC3ER, AND M, JEGU. NedtropicaliMonogehoidea/lS. An- — cyrocephalinae (Dactylogyridae) of Piranha and Their Relatives (Teleostei, JSer- rasalmidae) from Brazil and French Guiana: Species of Notozothecium Boeger and Kritsky, 1988, and Mymarotheciumgem. n. ..__ _______ __, ________ ..x,- ______ .s.... A. KOHN, C. P. SANTOS, AND-B. LEBEDEV. Metacdmpiella euzeti gen. n., sp, n., and I -Hargicola oligoplites~(Hargis, 1951) (Monogenea: Allodiscpcotylidae) from Bra- " zilian Fishes . ___________ ,:...L".. _______ j __ L'. _______l _; ________ 1 ________ _ __________ ______ _ .' _____ . __.. 176 C. P. SANTO?, T. SOUTO-PADRGN, AND R. M. LANFREDI. Atriasterheterodus (Levedev and Paruchin, 1969) and Polylabris tubicimts (Papema and Kohn, 1964) (Mono- ' genea) from Diplodus argenteus (Val., 1830) (Teleostei: Sparidae) from Brazil 181 . I...N- CAIRA AND T. BARDOS. Further Information on :.Gymnorhynchus isuri (Trypa- i/:norhyncha: Gymnorhynchidae) from the Shortfin Make Shark ...,.'. ..^_.-"_~ ____. ; 188 O. M. AMIN AND W. L.'MmcKLEY. Parasites of Some Fisji Introduced into an Arizona Reservoir, with Notes on Introductions — . ____ : ______ . ___.i;__ L____ _ . ______ :___ _ .193 O. M. AMIN AND O. SEY. Acanthocephala from Arabian Gulf Fishes off Kuwait, with 'Descriptions of Neoechinorhynchus dimorphospinus sp. n. XNeoechinorhyrichi- dae), Tegorhyrichus holospinosus sp. n. (l\lio&&ntid&e),:Micracanthorynchina-ku- waitensis sp. n. (Rhadinorhynchidae), and Sleriidrorhynchus breviclavipraboscis gen. n., sp. p. (Diplosentidae); and Key to Species of the Genus Micracanthor- .
    [Show full text]
  • ISSN: 2320-5407 Int. J. Adv. Res. 5(3), 972-999 REVIEW ARTICLE ……………………………………………………
    ISSN: 2320-5407 Int. J. Adv. Res. 5(3), 972-999 Journal Homepage: - www.journalijar.com Article DOI: 10.21474/IJAR01/3597 DOI URL: http://dx.doi.org/10.21474/IJAR01/3597 REVIEW ARTICLE HAEMONCHUS CONTORTUS AND OVINE HOST: A RETROSPECTIVE REVIEW. *Saeed El-Ashram1,2, Ibrahim Al Nasr3,4, Rashid mehmood5,6, Min Hu7, Li He7, *Xun Suo1 1. National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. 2. Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt. 3. College of Science and Arts in Unaizah, Qassim University, Unaizah, Saudi Arabia. 4. College of Applied Health Sciences in Ar Rass, Qassim University, Ar Rass 51921, Saudi Arabia. 5. College of information science and technology, Beijing normal university, Beijing, china. 6. Department of Computer Science and Information Technology, University of Management Sciences and Information Technology, Kotli Azad Kashmir, 11100, Pakistan 7. State Key Laboratory of Agricultural Microbiology, Key Laboratory of Development of Veterinary Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei,China. …………………………………………………………………………………………………….... Manuscript Info Abstract ……………………. ……………………………………………………………… Manuscript History Gastrointestinal (GI) parasitic infections are a world-wide problem for Received: 05 January 2017 both small- and large-scale farmers. Infection by GI parasites in Final Accepted: 09 February 2017 ruminants, including sheep and goat can result in harsh economic losses Published: March 2017 in a variety of ways: reproductive inefficiency, decreased work capacity, involuntary culling, diminished food intake, poor animal growth rates and lower weight gains, treatment and management costs, Key words:- Gastrointestinal (GI) parasitic infections; and mortality in heavily parasitized animals.
    [Show full text]
  • Frogs As Host-Parasite Systems I Frogs As Host-Parasite Systems I
    Frogs as Host-Parasite Systems I Frogs as Host-Parasite Systems I An Introduction to Parasitology through the Parasites of Rana temporaria, R. esculenta and R. pipiens 1. D. Smyth* and M. M. Smyth * Department of Zoology and Applied Entomology Imperial College, Unirersity of London M © J. D. Smyth and M. M. Smyth 1980 Softcover reprint of the hardcover 1st edition 1980978-0-333-28983-9 All rights reserved. No part of this publication may be reproduced or transmitted, in any form or by any means, without permission First published 1980 by THE MACMILLAN PRESS LTD London and Basingstoke Associated companies in Delhi Dublin Hong Kong Johannesburg Lagos Melbourne New York Singapore and Tokyo British Library Cataloguing in Publication Data Smyth, James Desmond Frogs as host-parasite systems. 1 1. Parasites-Frogs I. Title II. Smyth, M M 597'.8 SF997.5.F/ ISBN 978-0-333-23565-2 ISBN 978-1-349-86094-4 (eBook) DOI 10.1007/978-1-349-86094-4 This book is sold subject to the standard conditions of the Net Book Agreement The paperback edition of this book is sold subject to the condition that it shall not, by way of trade or otherwise, be lent, reso~.. hired out, or otherwise circulated without the publisher s prior consent in any form of binding or cover other than that in which it is published and without a similar condition including this condition being imposed on the subsequent purchaser Contents Introduction and Aims vii 2.3 Protozoa in the alimentary canal 7 2.4 Protozoa in the kidney 13 Acknowledgements IX 2.5 Protozoa in the blood 14 3.
    [Show full text]
  • Description of Enterobius (Colobenterobius) Emodensis Sp. N
    Zootaxa 4514 (1): 065–076 ISSN 1175-5326 (print edition) http://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2018 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.4514.1.5 http://zoobank.org/urn:lsid:zoobank.org:pub:C9C5FC4C-4402-4FA0-BBE7-0814172BE2C0 Description of Enterobius (Colobenterobius) emodensis sp. n. (Nematoda: Oxyuridae) collected from Central Himalayan langur, Semnopithecus schistaceus, in Uttarakhand, India HIDEO HASEGAWA1,4, HIMANI NAUTIYAL2, MIZUKI SASAKI3 & MICHAEL A. HUFFMAN2 1Department of Biomedicine / Department of Infectious Disease Control, Faculty of Medicine, Oita University, Hasama, Yufu, Oita 879–5593, Japan. E-mail: [email protected] 2Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. E-mail: [email protected]; [email protected] 3Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan. E-mail: [email protected] 4Corresponding author. E-mail: [email protected] Abstract A new pinworm species, Enterobius (Colobenterobius) emodensis sp. n. (Nematoda: Oxyuridae) is described from the Central Himalayan langur, Semnopithecus schistaceus, in Mandal Valley, Chamoli District, Uttarakhand, India, based on mature and immature adults and fourth-stage larvae. This species closely resembles Enterobius (Colobenterobius) zakiri parasitic in Tarai langur, Semnopithecus hector, recorded from Uttarakhand and Uttar Pradesh, India, but is readily distin- guished by having a shorter esophagus and a shorter spicule. It is surmised that this pinworm has co-speciated with the host langur. The new species is also characterized in that the posterior 1/3 of the esophageal corpus is much darker. Phy- logenetic analysis based on the sequences of partial Cox1 gene of mtDNA suggested a basal position of diversification of Colobenterobius from the Enterobius lineage.
    [Show full text]
  • The Helminthological Society O Washington
    VOLUME 9 JULY, 1942 NUMBER 2 PROCEEDINGS of The Helminthological Society o Washington Supported in part by the Brayton H . Ransom Memorial Trust Fund EDITORIAL COMMITTEE JESSE R. CHRISTIE, Editor U . S . Bureau of Plant Industry EMMETT W . PRICE U. S. Bureau of Animal Industry GILBERT F. OTTO Johns Hopkins University HENRY E. EWING U. S . Bureau of Entomology DOYS A. SHORB U. S. Bureau of Animal Industry Subscription $1 .00 a Volume; Foreign, $1 .25 Published by THE HELMINTHOLOGICAL SOCIETY OF WASHINGTON VOLUME 9 JULY, 1942 NUMBER 2 PROCEEDINGS OF THE HELMINTHOLOGICAL SOCIETY OF WASHINGTON The Proceedings of the Helminthological Society of Washington is a medium for the publication of notes and papers in helminthology and related subjects . Each volume consists of 2 numbers, issued in January and July . Volume 1, num- ber 1, was issued in April, 1934 . The Proceedings are intended primarily for the publication of contributions by members of the Society but papers by persons who are not members will be accepted provided the author will contribute toward the cost of publication . Manuscripts may be sent to any member of the editorial committee . Manu- scripts must be typewritten (double spaced) and submitted in finished form for transmission to the printer . Authors should not confine themselves to merely a statement of conclusions but should present a clear indication of the methods and procedures by which the conclusions were derived . Except in the case of manu- scripts specifically designated as preliminary papers to be published in extenso later, a manuscript is accepted with the understanding that it is not to be pub- lished, with essentially the same material, elsewhere .
    [Show full text]
  • Population Genetics, Community of Parasites, and Resistance to Rodenticides in an Urban Brown Rat (Rattus Norvegicus) Population
    RESEARCH ARTICLE Population genetics, community of parasites, and resistance to rodenticides in an urban brown rat (Rattus norvegicus) population AmeÂlie Desvars-Larrive1, Michel Pascal2², Patrick Gasqui3, Jean-FrancËois Cosson4,5, Etienne BenoõÃt6, Virginie Lattard6, Laurent Crespin3, Olivier Lorvelec2, BenoõÃt Pisanu7, Alexandre TeynieÂ3, Muriel Vayssier-Taussat4, Sarah Bonnet4, Philippe Marianneau8, Sandra Lacoà te8, Pascale Bourhy9, Philippe Berny6, Nicole Pavio10, Sophie Le Poder10, Emmanuelle Gilot-Fromont11, Elsa Jourdain3, Abdessalem Hammed6, Isabelle Fourel6, Farid Chikh12, GwenaeÈl Vourc'h3* a1111111111 a1111111111 1 Conservation Medicine, Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria, 2 Joint Research Unit (JRU) E cologie et Sante des E cosystèmes (ESE), Institut National de la a1111111111 Recherche Agronomique, INRA, Agrocampus Ouest, Rennes, France, 3 Joint Research Unit (JRU) a1111111111 EpideÂmiologie des Maladies Animales et Zoonotiques (EPIA), Institut National de la Recherche Agronomique, a1111111111 INRA, VetAgro Sup, Saint-Genès Champanelle, France, 4 Joint Research Unit (JRU) Biologie MoleÂculaire et Immunologie Parasitaire (BIPAR), Agence Nationale de SeÂcurite Sanitaire de l'Alimentation, de l'Environnement et du Travail (ANSES), Institut National de la Recherche Agronomique, INRA, Ecole Nationale VeÂteÂrinaire d'Alfort (ENVA), Maisons-Alfort, France, 5 Joint Research Unit (JRU) Centre de Biologie pour la Gestion des Populations (CBGP), Centre de CoopeÂration Internationale en Recherche Agronomique pour le DeÂveloppement (CIRAD), Institut National de la Recherche Agronomique, INRA, Institut OPEN ACCESS de Recherche pour le DeÂveloppement (IRD), SupAgro Montpellier, France, 6 Contract-based Research Unit (CBRU) Rongeurs Sauvages±Risques Sanitaires et Gestion des Populations (RS2GP), VetAgro Sup, Citation: Desvars-Larrive A, Pascal M, Gasqui P, Institut National de la Recherche Agronomique, INRA, Lyon University, Marcy-L'Etoile, France, 7 Unite Cosson J-F, BenoõÃt E, Lattard V, et al.
    [Show full text]
  • Aus Dem Institut Für Parasitologie Der Veterinärmedizinischen Fakultät Der Universität Leipzig
    Aus dem Institut für Parasitologie der Veterinärmedizinischen Fakultät der Universität Leipzig Feldstudien zum Vorkommen von Endoparasiten bei Neuweltkameliden in Ecuador Inaugural-Dissertation zur Erlangung des Grades eines Doctor medicinae veterinariae (Dr. med. vet.) durch die Veterinärmedizinische Fakultät der Universität Leipzig eingereicht von Anneliese Gareis – Waldburg aus Wien Leipzig, 2008 Mit Genehmigung der Veterinärmedizinischen Fakultät der Universität Leipzig Dekan : Prof. Dr. Dr.h.c. Karsten Fehlhaber Betreuer : Prof. Dr. Arwid Daugschies Gutachter : Prof. Dr. Arwid Daugschies, Institut für Parasitologie, Veterinärmedizinische Fakultät, Universität Leipzig Prof. Dr. Kurt Pfister, Institut für Vergleichende Tropenmedizin und Parasitologie, Ludwig-Maximilians-Universität München PD Dr. Thomas Wittek, Medizinische Tierklinik der Veterinärmedizinischen Fakultät, Universität Leipzig Tag der Verteidigung : 17. Juni 2008 Meinem Vater I Inhaltsverzeichnis 1 EINLEITUNG 1 2 LITERATURÜBERSICHT 2 2.1 Neuweltkameliden in Südamerika 2 2.1.1 Zuordnung und Rassebildung 2 2.1.2 Wirtschaftliche Nutzung von Kameliden in den Andenländern 3 2.1.3 Neuweltkameliden in Ecuador 4 2.2 Endoparasitosen bei Neuweltkameliden 4 2.2.1 Kokzidien 5 2.2.1.1 Allgemeines, Arten 5 2.2.1.2 Bekämpfungsprogramm bei Kokzidiose 10 2.2.2 Trematoden 11 2.2.2.1 Fasciola hepatica 11 2.2.2.2 Bekämpfung von Fasciola hepatica 13 2.2.3 Zestoden 14 2.2.3.1 Moniezia 14 2.2.3.2 Thysaniezia 16 2.2.3.3 Bekämpfung von Moniezia spp . und Thysaniezia spp. 16 2.2.3.4 Bandwurmfinnen 16 2.2.4 Nematoden 18 2.2.4.1 Allgemeine Angaben 18 2.2.4.2 Trichuris spp. 22 2.2.4.3 Capillaria spp.
    [Show full text]
  • Kenai National Wildlife Refuge Species List, Version 2018-07-24
    Kenai National Wildlife Refuge Species List, version 2018-07-24 Kenai National Wildlife Refuge biology staff July 24, 2018 2 Cover image: map of 16,213 georeferenced occurrence records included in the checklist. Contents Contents 3 Introduction 5 Purpose............................................................ 5 About the list......................................................... 5 Acknowledgments....................................................... 5 Native species 7 Vertebrates .......................................................... 7 Invertebrates ......................................................... 55 Vascular Plants........................................................ 91 Bryophytes ..........................................................164 Other Plants .........................................................171 Chromista...........................................................171 Fungi .............................................................173 Protozoans ..........................................................186 Non-native species 187 Vertebrates ..........................................................187 Invertebrates .........................................................187 Vascular Plants........................................................190 Extirpated species 207 Vertebrates ..........................................................207 Vascular Plants........................................................207 Change log 211 References 213 Index 215 3 Introduction Purpose to avoid implying
    [Show full text]