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(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2012/159639 Al 29 November 2012 (29.11.2012) (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, A23L 1/30 (2006.01) A61K 36/48 (2006.01) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, A61K 36/185 (2006.01) A61K 36/8962 (2006.01) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, A61K 36/63 (2006.01) A61K 36/54 (2006.01) HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, A61K 36/23 (2006.01) A61K 36/71 (2006.01) KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, A61K 36/9066 (2006.01) A61K 36/886 (2006.01) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, A61K 36/28 (2006.01) A61K 36/53 (2006.01) OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, A61K 36/82 (2006.01) A61K 36/64 (2006.01) SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, A61K 36/67 (2006.01) TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (21) International Application Number: (84) Designated States (unless otherwise indicated, for every PCT/EG20 12/0000 18 kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, (22) International Filing Date: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 22 May 2012 (22.05.2012) TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (25) Filing Language: English EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (26) Publication Language: English TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, (30) Priority Data: ML, MR, NE, SN, TD, TG). 201 1050814 24 May 201 1 (24.05.201 1) EG Declarations under Rule 4.17 : (72) Inventor; and — of inventorship (Rule 4.17(iv)) (71) Applicant : YOUNES, Abdel, Khalek, Hassan [EG/EG]; 50 Al Kalefa Al-Mamoon, Roxy - Heliopolis, Post Code Published: 1134 1, Cairo (EG). — with international search report (Art. 21(3)) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (54) Title: FOOD SUPPLEMENT FOR THERAPY OF VIRUS C (57) Abstract: This medicine is a nutritional supplement consisting of medicinal plants and natural herbs in the form of capsules, packed with raw material. Through which patients can regain their health so they can work and their daily lives normally, by strengthening their immune systems. In this way, the body can resist the virus of hepatitis C by restoring the balance of the immune system, enabling it to produce copious amounts of interferon by lymphocytes. Food supplement for therapy of virus C 2-Technical field Medical therapy 3-Background Art 1 - Previous treatment. A - Interferon treatment. The long-acting interferon is the drug situation in Egypt for the treatment of hepatitis c virus. Dosage: the patient gives an 48 injection IM for a year. In some cases, Ribavrin are given by mouth with interferon muscular injection. B - Supportive drugs for liver function as shown in four tables which includes more than 80 drugs, each table contains : 1 - Name of the drug user. 2 - Dose used. 3 - Price per package. 4 -Active ingredient in each drug. 5 - In addition to the name of Company. 4-Disclosure of the invention Detailed description of the plant used: 1 - The identification of the raw herb or herbal compendium including the Latin name and the user is part grass, supported by studies documented the safety and effectiveness. 2 - Specification of raw grass or feed. 3 - Calibration of grass or feed containing crude method of analysis and the physical and chemical specifications. 1 - Artichokes: A - herb Artichokes: Artichokes is Aharhov or thorns, ground floor, with other names, including: Knker, and Kenjr, and Canar, andJnarh, this herb is great¬ like plant spinal home is the regions ofsouthern Europe and North America and the Canary Islands, and the Middle East, the Mediterranean and are used leaves a medical herb. And roots and flowering heads of immature can also contain useful medical compounds. B - Latin name: artichoke (Cynara scolymus) C - part grass Username: each grass D - studies documented the safety and effectiveness by: Reference 1- Huber, R., et al. "Artichoke leave extract for chronic hepatitis C - a pilot study." Phytomedicine ; 2009 Sep; 16(9): 801-4. 2-Miccadei, S.,et al., "Antioxidative and apoptotic properties of polyphenolic extracts from edible part of artichoke( Cynara scolymus L.) on cultured rat hepatocytes and on human hepatoma cells." Nutr. Cancer . 2008; 60(2):276- 83. 3 - Valerio F, De Bellis P, Lonigro SL, et al. In vitro and in vivo survival and transit tolerance of potentially probiotic strains carried by artichokes in the gastrointestinal tract. Appl Environ.Microbiol. 2006;72(4):3042-3045. 4- Bundy R, Walker AF, Middleton RW, et al. Artichoke leaf extract reduces symptoms of irritable bowel syndrome and improves quality of life in otherwise healthy volunteers suffering from concomitant dyspepsia: a subset analysis. J Altern. Complement Med 2004; 10(4):667-669. View Abstract 5- Betancor-Fernandez A, Perez-Galvez A, Sies H, et al. Screening pharmaceutical preparations containing extracts of turmeric rhizome, artichoke leaf, devil's claw root and garlic or salmon oil for antioxidant capacity. J Pharm Pharmacol 2003 ;55(7):98 1-986. 6 - Jimenez-Escrig A, Dragsted LO, Daneshvar B, et al. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats. J Agric.Food Chem. 8-27-2003;51(18):5540-5545. 7- Speroni, E., et al. "Efficacy of different Cynara scolymus preparations on liver complaints." J. Ethnopharmacol .2003 Jun; 86(2-3): 203-1 1 E - Raw artichoke herb: grass is dry and the paper was used all of its components F- Physical and chemical specifications of the herb artichokes, which contain in all one hundred grams on: Source: USDA Nutrient database 2- Milky Thistle: A - Milky Thistle herb: There are thorns milky plant, or Shweikeh Mary in the wilderness in the area of the Mediterranean basin as found in Europe, and rarely found in England. There are herb on the sides of roads, and near swamps, sunny and humid places. And dry flower seeds used medically, and gathering fresh flowers are used in eating Kmqo year and a tonic for the body,such as cooked artichoke or land truck. Thistle and the use of lactic medically due to the more than 2000 years have passed, where she was dealing with some cases of depression, liver disease caused by alcohol, or various toxins.There are also other varieties of thorns, but it's not in the strength of the Milky medical thorns. The pharmacist said known in the seventeenth century, Nicholas Culpeper herb used to open blockages of the liver and spleen and recommended it even for the treatment ofjaundice, also pointed out that the forks handles cases of lactic (Almnjuli) associated with cirrhosis of the liver B - Latin name : Milk Thistle (Silybum Marianum) C - part grass Username: each grass D - studies documented the safety and effectiveness: Reference 1-Wagoner J, Negash A, Kane OJ, et al. Multiple effects of silymarin on the hepatitis C virus lifecycle. Hepatology. 2010;51(6): 1912-1921. 2-Lah JJ, Cui W, Hu KQ. Effects and mechanisms of silibinin on human hepatoma cell lines. World J Gastroenterol 2007; 13(40): 5299-5305 3-Agarwal R, Agarwal C, Ichikawa H, Singh RP, Aggarwal BB. Anticancer potential of silymarin: from bench to bed side. Anticancer Res. 2006 Nov-Dec;26(6B):4457-98. Review. 4-Gordon A, Hobbs DA, Bowden DS, Bailey MJ, Mitchell J, Francis AJ, Roberts SK. Effects of Silybum marianum on serum hepatitis C virus RNA, alanine aminotransferase levels and well-being in patients with chronic hepatitis C. J Gastroenterol Hepatol. 2006 Jan;21(l Pt 2):275-80. 5-Varghese, Leyon, et al., Silibinin Efficacy Against Human Hepatocellular Carcinoma, Clinical Cancer Research, December 1, 2005 6-Mayer KE, Myers RP, Lee SS. Silymarin treatment of viral hepatitis: a systematic review. ViralHepat. 2005 Nov; 12(6):559-67. Review. 7-Zielinska-Przyjemska M, Wiktorowicz K. An in vitro study of the protective effect of the flavonoid silydianin against reactive oxygen species. Phytother Res. 2006 Feb;20(2):115-9 New Research for liver cancer: Published in the October 28, 2007 issue of WorldJournal of Gastroenterology, Dr. Ke-Qin Hu and his research team from the University of California, Irvine, demonstrated that silybin demonstrates anti-cancer effects. Dr. Hu has published over 70 scientific articles in various medical professional journals; many of which focus on viral Hepatitis B and C, cirrhosis and liver cancer. Indicating milk thistle's broad spectrum of anti-liver cancer effects, the researchers found that silybin can significantly reduce the growth of four different human liver cancer cell lines. In addition, they demonstrated that silybin exhibits its anti-liver cancer effects by: 1-Reducing cancer cell proliferation and cell cycle progression 2. Enhancing programmed death of cancer cells 3. Altering chromatin structure of the cancer cells. The results of this study indicate that silybin may be useful in preventing the development of liver cancer. Although conducted in a laboratory, this purified form of milk thistle displays obvious mechanisms against HCC.
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  • Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MA

    Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MA

    Int. J. Biol. Sci. 2016, Vol. 12 72 Ivyspring International Publisher International Journal of Biological Sciences 2016; 12(1): 72-86. doi: 10.7150/ijbs.13188 Research Paper Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MAPK and NF-κB and Activating HO-1/Nrf2 Pathways Hongming Lv1#, Zhenxiang Yu2#, Yuwei Zheng1, Lidong Wang1, Xiaofeng Qin1, Genhong Cheng1, Xinxin Ci1 1. Institute of Translational Medicine, The First Hospital of Jilin University, College of Veterinary Medicine, Jilin University, Changchun, China 2. Department of Respiratory Medicine, The First Hospital of Jilin University, Changchun, China. # These authors contributed equally to this work. Corresponding author: X.-X.Ci, Institute of Translational Medicine, The First Hospital, Jilin University, Changchun, 130001, China. Tel.: +86 431 88783044; E-mail addresses: [email protected] (X.-X.Ci.) © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. Received: 2015.07.08; Accepted: 2015.11.02; Published: 2016.01.01 Abstract Oxidative damage and inflammation are closely associated with the pathogenesis of acute lung injury (ALI). Thus, we explored the protective effect of isovitexin (IV), a glycosylflavonoid, in the context of ALI. To accomplish this, we created in vitro and in vivo models by respectively exposing macrophages to lipopolysaccharide (LPS) and using LPS to induce ALI in mice. In vitro, our results showed that IV treatment reduced LPS-induced pro-inflammatory cytokine secretion, iNOS and COX-2 expression and decreased the generation of ROS.
  • Potential Role of Flavonoids in Treating Chronic Inflammatory Diseases with a Special Focus on the Anti-Inflammatory Activity of Apigenin

    Potential Role of Flavonoids in Treating Chronic Inflammatory Diseases with a Special Focus on the Anti-Inflammatory Activity of Apigenin

    Review Potential Role of Flavonoids in Treating Chronic Inflammatory Diseases with a Special Focus on the Anti-Inflammatory Activity of Apigenin Rashida Ginwala, Raina Bhavsar, DeGaulle I. Chigbu, Pooja Jain and Zafar K. Khan * Department of Microbiology and Immunology, and Center for Molecular Virology and Neuroimmunology, Center for Cancer Biology, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19129, USA; [email protected] (R.G.); [email protected] (R.B.); [email protected] (D.I.C.); [email protected] (P.J.) * Correspondence: [email protected] Received: 28 November 2018; Accepted: 30 January 2019; Published: 5 February 2019 Abstract: Inflammation has been reported to be intimately linked to the development or worsening of several non-infectious diseases. A number of chronic conditions such as cancer, diabetes, cardiovascular disorders, autoimmune diseases, and neurodegenerative disorders emerge as a result of tissue injury and genomic changes induced by constant low-grade inflammation in and around the affected tissue or organ. The existing therapies for most of these chronic conditions sometimes leave more debilitating effects than the disease itself, warranting the advent of safer, less toxic, and more cost-effective therapeutic alternatives for the patients. For centuries, flavonoids and their preparations have been used to treat various human illnesses, and their continual use has persevered throughout the ages. This review focuses on the anti-inflammatory actions of flavonoids against chronic illnesses such as cancer, diabetes, cardiovascular diseases, and neuroinflammation with a special focus on apigenin, a relatively less toxic and non-mutagenic flavonoid with remarkable pharmacodynamics. Additionally, inflammation in the central nervous system (CNS) due to diseases such as multiple sclerosis (MS) gives ready access to circulating lymphocytes, monocytes/macrophages, and dendritic cells (DCs), causing edema, further inflammation, and demyelination.