agronomy Article Morphological, Genetic and Biochemical Evaluation of Dasypyrum villosum (L.) P. Candargy in the Gene Bank Collection VojtˇechHolubec 1,* ,Václav Dvoˇráˇcek 2 , Leona Svobodová Leišová 3 and Sezai Ercisli 4 1 Department of Gene Bank, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech Republic 2 Department of Product Quality, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech Republic;
[email protected] 3 Department of Molecular Biology, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech Republic;
[email protected] 4 Department of Horticulture, Agricultural Faculty, Ataturk University, Erzurum 25240, Turkey;
[email protected] * Correspondence:
[email protected]; Tel.: +42-02-3302-2497 Abstract: The Dasypyrum villosum gene bank collection, comprising 32 accessions, was characterized morphologically and genetically for resistance to leaf diseases and for quality parameters of seeds with specific accent to protein polymorphism and protein and starch composition. The collected material represented nearly the whole distribution area in the Mediterranean. For SSR analysis, a set of 40 SSR markers for wheat was selected. A matrix of distances between genotypes was calculated using Simple Matching dissimilarity coefficient in the DARwin software. The collection was scored for resistance to powdery mildew, brown, stripe and stem rusts. A modified SDS-PAGE method Citation: Holubec, V.; Dvoˇráˇcek,V.; with clear interpretation of high and low molecular glutenin subunits (HMW, LMW) was used for Svobodová Leišová, L.; Ercisli, S. Morphological, Genetic and characterization of accessions. Morphological phenotyping revealed considerable diversity allowing Biochemical Evaluation of Dasypyrum the distinguishing of clusters tracing the geographical origin of accessions. Genetic diversity showed villosum (L.) P.