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Kmcb-Abstract-Book-2019-Final VIII April 27 – May 1, 2019 2 Eight Kinetoplastid Molecular Cell Biology Meeting April 27 – May 1, 2019 Hosted by the Marine Biological Laboratory Woods Hole, Massachusetts, USA The meeting was founded by George A.M. Cross in 2005 Organizers George A.M. Cross 2005-2011 Christian Tschudi 2013-2019 3 KMCBM 2019 Acknowledgements The organizer wishes to thank: The Program Committee Barbara Burleigh (Harvard T. H. Chan School of Public Health, Boston, USA) James D. Bangs (University at Buffalo, Buffalo, USA) Stephen M. Beverley (Washington University School of Medicine, St. Louis, USA) Keith R. Matthews (The University of Edinburgh, Edinburgh, Scotland, UK) The Staff at MBL: Paul Anderson and the MBL Housing and Conference Staff for registration and housing; All the IT AV Support staff and the staff in Sodexo Food Service at the MBL. Cover Design: Markus Engstler 4 KMCBM 2019 Program Saturday, April 27 02:00 – 05:00 Arrival, Registration and Poster Session A setup 04:00 – 06:30 Greeting and Dinner 07:00 – 09:00 Session I: VSG (chair: Mark Carrington) 09:00 – 11:00 Mixer Sunday, April 28 07:00 – 08:30 Breakfast 08:45 – 11:45 Session II: Biochemistry/Metabolism (chair: Ken Stuart) 12:00 – 01:30 Lunch 02:00 – 04:30 Session III: Cell Biology (chair: Kimberly Paul) 06:00 – 07:00 Dinner 07:00 – 09:00 POSTER PRESENTATIONS: Session A 09:00 – 11:00 Mixer & Poster A/B Changeover Monday, April 29 07:00 – 08:30 Breakfast 08:45 – 11:45 Session IV: Pathogenesis I (chair: Luisa Figueiredo) 12:00 – 01:30 Lunch 01:30 – 06:00 Free Time 06:00 – 07:00 Dinner 07:00 – 09:00 POSTER PRESENTATIONS: Session B 09:00 – 11:00 Mixer & Poster B/C Changeover 5 Tuesday, April 30 07:00 – 08:30 Breakfast 08:45 – 11:45 Session V: Cell Cycle/Cytokenisis (chair: Michele Klingbeil) 12:00 – 01:30 Lunch 02:00 – 04:30 Session VI: Gene Expression (chair: Ruslan Afasizhev) 06:00 – 07:00 Dinner 07:00 – 09:00 POSTER PRESENTATIONS: Session C 09:00 – 11:00 Mixer Wednesday, May 1 07:00 – 08:30 Breakfast 08:45 – 11:15 Session VII: Pathogenesis II (chair: Megan Povelones) 12:00 – 01:30 Lunch or lunch boxes ROOMS MUST BE VACATED BY 10:00 AM ON WEDNESDAY 6 In Memoriam: Paul T. Englund, Ph.D. 1938-2019 C.C. Wang, Ph.D. 1936-2017 7 8 SESSION I: VSG Mark Carrington, Chair. Saturday 7:00 pm 04/27/19 9 1A Identification of novel ESB associated proteins in bloodstream form Trypanosoma brucei Budzak, James Jones, Robert (Sir Alexander Fleming Building, Dept. of Life Sciences, Imperial College London, U.K.); Tschudi, Christian; Kolev, Nikolay G. (Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06536, USA.); Rudenko, Gloria (Sir Alexander Fleming Building, Dept. of Life Sciences, Imperial College London, U.K.) 1B A chromatin-associated VEX/CAF-1 complex drives VSG single gene choice and epigenetic inheritance in Trypanosoma brucei Faria, Joana Glover, Lucy; Hutchinson, Sebastian (Institut Pasteur Paris); Boehm, Cordula; C. Field, Mark; Horn, David (Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee) 1C m6A methylation of the polyA-tail regulates VSG transcript stability in trypanosomes Viegas, Idalio Rodrigues, João; Macedo, Juan (Instituto de Medicina Molecular); Aresta Branco, Francisco (Division of Immune Diversity, German Cancer Research Center, Heidelberg, Germany.); Jaffrey, Samie (Department of Pharmacology, Weill Cornell Medicine, Cornell University); Figueiredo, Luisa (Instituto de Medicina Molecular) 1D Trypanosoma brucei RAP1 has an RNA binding activity that is essential for VSG monoallelic expression Gaurav, Amit K. Afrin, Marjia (Cleveland State University); Yang, Xian; Pan, Xuehua (The Hong Kong Polytechnic University); Sandhu, Ranjodh (University of California, Davis); Zhao, Yanxiang (The Hong Kong Polytechnic University); Li, Bibo (Cleveland State University) 1E Active expression site ESAGs are not essential in vitro, but their presence alters VSG switching Chamberlain, James Gadelha, Catarina (University of Nottingham, Nottingham, United Kingdom) 1F Developmental competence and antigen switch frequency can be uncoupled in Trypanosoma brucei McWilliam, Kirsty Mugnier, Monica (Johns Hopkins University); Morrison, Liam; Matthews, Keith (University of Edinburgh) TT1G TURNOVER OF VSG IN TRYPANOSOMA BRUCEI Garrison, Paige Umaer, Khan; Bush, Peter; Bangs, Jay (University at Buffalo) TT1H How DNA breaks contribute to mosaic VSG formation in Trypanosoma brucei? Romero-Meza, Gabriela Mugnier, Monica (Johns Hopkins Bloomberg School of Public Health) 10 SESSION II: Biochemistry/Metabolism Ken Stuart, Chair. Sunday 8:45 am 04/28/19 11 2A Glycerol, a new key player in the central carbon metabolism of African trypanosomes Bringaud, Frédéric MFP (CNRS UMR-5234), University of Bordeaux, Bordeaux, France 2B The Trypanosoma UDP-glucose pyrophosphorylase is active in the cytosol and glycosomes and is imported into the organelle by piggybacking Villafraz, Oriana; Baudouin, Hélène; Mazet, Muriel (MFP (CNRS UMR-5234), University of Bordeaux, Bordeaux, France); Kulyk, Hanna; Portais, Jean-Charles (LISBP (CNRS, INRA, INSA), University of Toulouse, Toulouse, France) 2C Responses to reduced glucose transport in Trypanosoma brucei: gluconeogenesis and signaling Kovarova, Julie Hutchinson, Sebastian; Horn, David (University of Dundee) 2D Trypanosoma cruzi intracellular amastigote glutamine metabolism and its impact on susceptibility to ergosterol biosynthesis inhibitors Dumoulin, Peter Burleigh, Barbara (Harvard T.H. Chan School of Public Health) 2E A foot in the door: Understanding nutrient stress tolerance and adaptive gene regulation in Leishmania donovani through the lens of a purine- responsive transporter Licon, Haley Soysa, Radika (University of Washington); Landfear, Scott; Yates, Phillip (Oregon Health and Science University) 2F Remodeling the mitochondrial proteome and metabolic networks of Trypanosoma brucei during in vitro insect stage differentiation Zikova, Alena Dolezelova, Eva (Biology Centre); Levin, Michal; Dejung, Mario (Institute of Molecular Biology); Kunzova, Michaela; Panicucci, Brian (Biology Centre); Janzen, Christian; Butter, Falk (University Wuerzburg) 2G Deciphering the mitochondrial quality control mechanism in Trypanosoma brucei Dewar, Caroline Oeljeklaus, Silke; Suppanz, Ida; Peikert, Christian (University of Freiburg); Mani, Jan (University of Bern); Warscheid, Bettina (University of Freiburg); Schneider, André (University of Bern) TT2H Targeting the ubiquitin system of Leishmania Mexicana Burge, Rebecca Damianou, Andreas; Catta-Preta, Carolina (The University of York); Rodenko, Boris (UbiQ); Mottram, Jeremy C. (The University of York) TT2I Identifying highly divergent glycosyltransferases in the African Trypanosome Duncan, Samuel Damerow, Manuela; Ferguson, Michael (The University of Dundee) 12 SESSION III: Cell Biology Kimberly Paul Sunday 2:00 pm 04/28/19 13 3A Cryo electron tomography with Volta phase plate reveals novel structural foundations of axoneme motility in the pathogen Trypanosoma brucei Imhof, Simon Zhang, Jiayan (Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, California, USA); Wang, Hui (California NanoSystems Institute, UCLA); Huy Bui, Khanh (Department of Anatomy and Cell Biology, McGill University, Montreal, CA); Nguyen, Hoangkim (Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, California, USA); Atanosov, Ivo; Hui, Wong; Zhou, Hong (California NanoSystems Institute, UCLA); Hill, Kent (Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, California, USA) 3B The Leishmania flagellum attachment zone is critical for flagellar pocket shape and development in the sand fly and pathogenicity in the host Sunter, Jack Yanase, Ryuji (University of Hyogo); Wang, Ziyin (University of Oxford); Catta- Preta, Carolina (University of York); Moreira-Leite, Flavia (University of Oxford); Myskova, Jitka; Pruzinova, Katerina; Volf, Petr (Charles University); Mottram, Jeremy (University of York); Gull, Keith (University of Oxford) 3C Structural studies of the interaction between FPC3 and FPC4, two components of the flagellar pocket collar in Trypanosoma brucei Pivovarova, Yulia Majneri, Paul; Lesigang, Johannes (Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Austria); Robinson, Derrick R.; Bonhivers, Melanie (CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France); Dong, Gang (Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Austria) 3D Characterization of TAP110, a mitochondrial protein associated with the TAC, in Trypanosoma brucei Amodeo, Simona Schimanski, Bernd; Ochsenreiter, Torsten (University of Bern, Switzerland) 3E Processing and targeting of cathepsin L (TbCatL) to the lysosome in Trypanosoma brucei Koeller, Carolina M. Bangs, James D. (University at Buffalo) TT3F Swim like your lifecycle depends on it: Investigating chemotaxis and navigation in Leishmania parasites Walrad, Pegine B. Findlay, Rachel; Osman, Mohamed (York Biomedical Research Institute); Gadelha, Hermes (Department of Mathematics); Kaye, Paul (York Biomedical Research Institute); Rogers, Matthew (London School of Hygeine and Tropical Medicine); Wilson, Laurence (Biological Physical Sciences Institute, University of York) TT3G The dual FAZ domain-localizing protein FAZ27 cooperates with FLAM3 and ClpGM6 to control morphology transitions in Trypanosoma brucei AN, TAI ZHOU, QING; HU, HUIQING; LI, ZIYIN (University of Texas Medical School)
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