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Mouse Anti-Calponin Catalog No Qty: 100 μg/200 µL Mouse anti-Calponin Catalog No. 414700 Lot No. Mouse Anti-Calponin FORM This affinity-purified mouse monoclonal antibody is supplied as a 200 µL aliquot at a concentration of 0.5 mg/mL in PBS, pH 7.4, containing 0.1% sodium azide. This antibody is highly purified from mouse ascites by protein A chromatography. Clone: N3-16 Isotype: IgG1 IMMUNOGEN Crude smooth muscle preparation from human uterus. SPECIFICITY This antibody is specific for the calponin-1 (smooth muscle basic calponin, calponin H1) protein (accession # P51911, NP_001290) which is 98% homologous with canine, bovine, swine and ferret, and 97% with rat and mouse sequences. On Western Blots of human2 and bovine aorta extract, it identifies the target band at ~34 kDa. REACTIVITY Reactivity has been confirmed with human2 and bovine aorta extract by Western Blotting, and with human aorta tissue2 by immunofluorescence . Based on amino acid sequence homology, reactivity with, bovine, canine, swine, ferret, rat and mouse is also expected. Sample Western Immuno- Immuno- Blotting Histochemistry fluorescence (frozen) Human +++ +++ (4) +++ (2) Bovine +++ ND ND Canine ND ND ND Swine ND ND ND Ferret ND ND ND Rat ND ND ND Mouse ND ND ND (Excellent +++, Good ++, Poor +, No reactivity 0, Not applicable N/A, Not determined ND) USAGE Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western Blotting: 2 µg/mL Immunofluorescence: 5-10 µg/mL (cont’) www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI414700 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. (414700 cont’d) STORAGE Store at 2-8°C for up to one month. Store at –20°C for long-term storage. Avoid repeated freezing and thawing. BACKGROUND Calponin is a 34 kDa actin binding protein, originally purified from vertebrate smooth muscle playing a regulatory role in the contractile machinery. Calponin inhibits actomyosin ATPase activity in vitro and co-immunoprecipitates and co-localizes with protein kinase C epsilon (PKC epsilon) and ERK1 in vascular smooth muscle cells (SMC). It has been shown that calponin, unlike other actin binding proteins which inhibit actomyosin ATPase activity (such as caldesmon and troponin), undergoes an agonist induced translocation from the cytoskeleton to the submembranous cortex.1 The expression of calponin, similar to the expression of alpha-SM-actin, SM-MHCs, and h-caldesmon, is developmentally regulated in aortic SMCs. Actin and myosin, being major contractile proteins, also play a structural role and appear rather early in development, whereas caldesmon and calponin, being involved in regulation of contraction, can serve as markers of higher SMC differentiation steps.2 Quantitative and/or qualitative alteration of actin cytoskeletal molecules, involved in the regulation of cellular dynamic functions, should be intimately related with cancer phenotypes. Based on several lines of experimental evidence, calponin h1, an actin-stabilizing molecule, can simultaneously attack cancer cells and protect the host from cancer invasion. Calponin h1 is very often suppressed in vascular smooth muscle cells of malignant human tumors and in mesothelial cells by coexisting cancer cells.3 REFERENCES 1. Leinweber B, et al. J Biol Chem 275(51):40329-36, 2000. 2. Frid MG, et al. Dev Biol 153(2):185-93, 1992. 3. Taniguchi S, et al.Cancer Sci 96(11):738-46. Review, 2005. 4. Lazard D, et al. Proc Natl Acad Sci U S A 90(3):999-1003, 1993. RELATED PRODUCTS Product Conjugate Cat. No. Protein A Sepharose 4B 10-1041 rec-Protein G Sepharose 4B 10-1241 ZyMAX™ Goat anti-rabbit IgG Unconjugated 81-6100 ZyMAX™ Goat anti-mouse IgG Unconjugated 81-6500 Secondary antibody conjugates. Goat anti-rabbit IgG Goat anti-mouse IgG Fluorescence Conjugate Ex/Em* (H+L) (H+L) similar to-- Alexa Fluor® 488 A11008 A11001 495/519 FITC Alexa Fluor® 555 A21428 A21422 555/565 Cy3 Alexa Fluor® 594 A11012 A11005 590/617 Texas Red Alexa Fluor® 647 A21244 A21235 650/668 Cy5 HRP 81-6120 81-6520 NA** NA AP 81-6122 81-6522 NA NA Biotin B2770 B2763 NA NA *Excitation/emission (nm); **Not applicable For additional secondary antibody conjugates, visit www.invitrogen.com/antibodies For Research Use Only www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI414700 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. .
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