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SnapShot: Actin Regulators I Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA Representative Proteins Protein Family Biochemical Function H. sapiens D. melanogaster C. elegans A. thaliana S. cerevisiae Actin Nucleators Formins associates with barbed ends, *hDia1, 2, 3, FHOD1, Diaphanous/Dia, Dia/CYK-1, DAAM, AtFH1-4, 5, 6-21 Bni1, Bnr1 promotes nucleation 2, 3, 4, DAAM1, 2, Cappucino/FMN, FRL, FHOD, INF FMN1, 2, FRL1, 2, 3, DAAM, FRL, FHOD, INF1, 2, Delphilin INF Spir nucleates unbranched fi laments Spir-1, Spir-2 Spir/spire Arp2/3 complex polymerizes Y-branched fi lament ARP2, ARP3, ARP2, ARP3, ARPC1, ARX1-7 ARP2, ARP3, ARPC1-5 Arp2, Arp3, Arc40, networks ARPC1A, 1B, 2, 3, 2, 3A, 3B, 4, 5, 5L 35, 18, 19, 15 4, 5, 5L CARMIL links capping protein, Arp2/3 and CARMIL DCarmil †Q21301 myosin I Coronin binds F-actin and Arp2/3 complex, coronin 1A, B, C; Coro/Coronin, Dpod1 COR-1, POD-1 Crn1 enhances actin depolymerization 2A, B; 7 Cortactin activates/modulates Arp2/3 complex, cortactin, HS1 DCortactin binds F-actin SCAR/WAVE activates Arp2/3 complex SCAR1, SCAR2, SCAR WVE-1 AtSCAR 1, 2, 3, 4 family SCAR3 Arp2/3 complex regulators WASP family activates Arp2/3 complex *WASP, N-WASP WASp WSP-1 Las17/Bee1 G-Actin Binding Proteins CAP/Srv2 recycles actin monomers CAP1, CAP2 Act up/Capulet CAS-1 AtCAP1 Srv2 Profi lin promotes actin monomer addition at profi lin 1, 2, 3, 4 Chickadee PFN1, 2, 3 PRF1-3 (vegetative), Pfy1 barbed ends PRF4-5 (reproductive) Thymosin β4 sequesters G-actin thymosin β4 Ciboulot TTH-1 Verprolin/WIP binds actin monomers, WASP WIP, WIRE/WICH, †Q8MLU0 WIP-1 Vrp1 CR16 F-Actin Binding Proteins Identifi ed Cellular Role: X Membrane traffi cking and phagocytosis Ena/VASP family antagonizes CapZ capping at fi lament Mena, VASP, EVL Enabled UNC-34 X Cytokinesis ends, recruits profi lin X Cell surface organization and dynamics MIM binds both G- and F-actin, may MIM X Cell adhesion regulate nucleation-promoting factors X Multiple functions Pointed End Capping Proteins X Other/unknown Tropomodulin/ caps pointed ends, binds tropomyosin Tmod1, 2, 3, 4, Sanpodo TMD-1, TMD-2 †UNIPROT database accession number Tmod Lmod1, 2, 3 *Mutation linked to human disease Barbed End Capping Proteins AIP1 promotes depolymerization by ADF/ AIP1/WDR1 Aip1 AIP-1/UNC-78 AtAIP1 Aip1 cofi lin CapG caps barbed ends (calcium- capG CapG dependent) CapZ/capping heterodimer caps barbed ends capZ (A1-3, B1-2) CapZ (cpa, cpb) CAP-1, CAP-2 AtCPA, AtCPB Cap1, Cap2 protein/β-actinin Eps8 caps barbed ends, involved in Rac Eps8, Eps8L1, 2, 3 Arouser, †Q95TJ6 EPS-8 signalling Actin Depolymerizing/Severing Proteins ADF/cofi lin promotes actin fi lament disassembly cofi lin1, cofi lin2, ADF Cofi lin/Twinstar UNC-60A, B AtADF1-12 Cof1 Gelsolin/villin severs actin fi laments gelsolin, adseverin/ Gelsolin, Villin-like/ gelsolin (†Q21253), VLN1-5 superfamily scinderin, villin, Quail, Flightless I supervillin advillin, supervillin, (†P91041), FLI-1 fl ightless I Twinfi lin inhibits actin nucleotide exchange, twinfi lin-1/A6, Twinfi lin †U46668 Twf1 severs actin twinfi lin-2 Actin Bundling/Crosslinking Proteins α-actinin connects and organizes actin fi laments α-actinin1-4 α-actinin/Flightless A ATN-1 EPLIN stabilizes and bundles actin fi laments EPLIN Espin forms parallel actin bundles *espin Forked Fascin bundles actin fi laments fascin 1, 2, 3 Singed Filamin orthogonally crosslinks actin fi laments fi lamin *A, *B, C Jitterbug, Cheerio †Q18109 Fimbrin/plastin bundles actin fi laments plastin 1-3 (I, L, T) Fimbrin †Q965Y6 AtFIM1 Sac6 See online version for references and abbreviations. Actin Regulators II will appear in the March 9 issue of Cell. 626 Cell 128, February 9, 2007 ©2007 Elsevier Inc. DOI 10.1016/j.cell.2007.02.001 SnapShot: Actin Regulators I Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA REFERENCES Aspenstrom, P. (2005). The verprolin family of proteins: Regulators of cell morphogenesis and endocytosis. FEBS Lett. 579, 5253–5259. Daly, R.J. (2004). Cortactin signalling and dynamic actin networks. Biochem. J. 382, 13–25. dos Remedios, C.G., Chhabra, D., Kekic, M., Dedova, I.V., Tsubakihara, M., Berry, D.A., and Nosworthy, N.J. (2003). Actin binding proteins: regulation of cytoskeletal microfilaments. Physiol. Rev. 83, 433–473. Goley, E.D., and Welch, M.D. (2006). The ARP2/3 complex: An actin nucleator comes of age. Nat. Rev. Mol. Cell Biol. 7, 713–726. Higgs, H.N., and Peterson, K.J. (2005). Phylogenetic analysis of the formin homology 2 domain. Mol. Biol. Cell 16, 1–13. Kerkhoff, E. (2006). Cellular functions of the Spir actin-nucleation factors. Trends Cell Biol. 16, 477–483. Krause, M., Dent, E.W., Bear, J.E., Loureiro, J.J., and Gertler, F.B. (2003). Ena/VASP proteins: Regulators of the actin cytoskeleton and cell migration. Annu. Rev. Cell Dev. Biol. 19, 541–564. Paavilainen, V.O., Bertling, E., Falck, S., and Lappalainen, P. (2004). Regulation of cytoskeletal dynamics by actin-monomer-binding proteins. Trends Cell Biol. 14, 386–394. Silacci, P., Mazzolai, L., Gauci, C., Stergiopulos, N., Yin, H.L., and Hayoz, D. (2004). Gelsolin superfamily proteins: Key regulators of cellular functions. Cell. Mol. Life Sci. 61, 2614–2623. Uetrecht, A.C., and Bear, J.E. (2006). Coronins: The return of the crown. Trends Cell Biol. 16, 421–426. ABBREVIATIONS ADF: actin-depolymerizing factor AIP1: actin-interacting protein 1 ARP: actin-related protein ARPC: actin-related protein 2/3 complex CAP1 and CAP2: cyclase-associated protein 1 and 2 CARMIL: capping protein, Arp2/3, and myosin linker DAAM: dishevelled-associated activator of morphogenesis Ena: Enabled EPLIN: epithelial protein lost in neoplasm Eps8: EGF receptor pathway substrate 8 EVL: Ena/VASP-like protein FHOD: formin homology-2 domain-containing protein FMN: formin FRL: formin-related gene in leukocytes hDia: human homolog of Drosophila Diaphanous INF: inverted formin Lmod: leiomodin Mena: mammalian enabled MIM: missing in metastasis N-WASP: neuronal Wiskott-Aldrich Syndrome protein SCAR/WAVE: suppressor of cAMP receptor/WASP family verprolin-homologous protein Tmod: tropomodulin VASP: vasodilator-stimulated phosphoprotein WASP: Wiskott-Aldrich Syndrome protein WIP: WASP-interacting protein WIRE/WICH: WIP-related/WIP and CR16 homologous protein 626.e1 Cell 128, February 9, 2007 ©2007 Elsevier Inc. DOI 10.1016/j.cell.2007.02.001 SnapShot: Actin Regulators II Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA Representative Proteins Protein Family H. sapiens D. melanogaster C. elegans A. thaliana S. cerevisiae Endocytosis and Exocytosis ABP1/drebrin mABP1, drebrin, drebrin- †Q95RN0 †Q9XUT0 Abp1 like EPS15 EPS15 Eps-15 EHS-1 †Q56WL2 Pan1 HIP1R HIP1R †Q8MQK1 †O62142 Sla2 Synapsin synapsin Ia, Ib, IIa, IIb, III Synapsin SNN-1 Plasma Membrane Association Anillin anillin Scraps ANI-1, 2, 3 Annexins annexin A1–11, 13 (actin Annexin B9-11 NEX-1–4 ANN1-8 binding: 1, 2, 6) ERM proteins ezrin, radixin, moesin DMoesin ERM-1 MARCKS MARCKS, MRP/ Akap200 MACMARCKS/F52 Merlin *merlin/NF2 Merlin NFM-1 Protein 4.1 4.1R, G, N, B Coracle Spectrin α-spectrin (1–2), β-spectrin α-spectrin, β-spectrin, β heavy- SPC-1 (α-spectrin), UNC-70 (1–4), β heavy-spectrin/ spectrin/Karst (β-spectrin), SMA-1 (β heavy- karst spectrin) Identifi ed Cellular Role: X Membrane traffi cking and phagocytosis Cell-Cell Junctions X Cytokinesis α-catenin α-catenin 1–3 α-catenin HMP-1 X Cell surface organization and dynamics X Cell adhesion Afadin afadin/AF6 Canoe AFD-1 X Multiple functions ZO-1 ZO-1, ZO-2, ZO-3 ZO-1/Polychaetoid †Q56VX4 X Other/unknown Cell-Extracellular Matrix Junctions †UNIPROT database accession number *Mutation linked to human disease Dystrophin/utrophin *dystrophin, utrophin/ Dystrophin DYS-1 DRP1, DRP2 LASP LASP-1, LASP-2, LIM- Lasp †P34416 nebulette Palladin palladin Parvin α-, β-, χ-parvin †Q9VWD0 PAT-6 (α-parvin) Plakins BPAG1, MACF1, plectin Shortstop/Kakapo VAB-10 †Q8S8J6, Q38843, Q9C7V7 Talin talin 1–2 Rhea/Tendrils †Q22860 Tensin tensin 1–3 Blistery/Tensin †P92160 Vinculin vinculin Vinculin DEB-1 Zyxin/paxillin zyxin, paxillin Zyx102, Dpax ZYX-1, paxillin (†Q09476) Motor Proteins and Regulators Caldesmon caldesmon Crtp Calponin calponin 1–3 Chd64 CPN-1–4 Scp1 Myosin family Myosin types 1, 2, 3, *5, *6, Myosin types 1, 2, 3, 5, 6, 7, Myosin types 1, 2, 5, 6, 7, 9, 12 Myosin types 8, 11 Myosin types 1, 2, 5 *7, 9, 10, *15, 16, 18, 19 15, 18, 20 Tropomyosin tropomyosin 1, *2, *3, 4 Tm1, Tm2 LEV-11/TMY-1 †Q8LE98 Tpm1, Tpm2 Troponin troponin T*1, *2, 3; C1, 2; Troponin T/Upheld, Troponin TNI-1-4, TNC-1-2, TNT-1-4 I1, 2, *3 I/Wings up, Troponin C1-3, Tina-1 Muscle Components Myopalladin myopalladin, *myotilin Nebulin *nebulin, *nebulette, N-RAP Nebulin †P34416 Titin/connectin *titin/connectin D-titin, Projectin/Twitchin Ce-titin, stretchin Nuclear Actin-Associated Proteins Chromatin-remodeling BAF, PBAF, TIP60, p400 BAP Ino80, NuA4 complexes hnRNP proteins DBP40 (R. norvegicus) hrp36, hrp65 (C. tentans) Nuclear DNA Helicase II NDHII Maleless/MLE RHA-1 See online version for references and abbreviations. Actin Regulators I appeared in the February 9 issue of Cell. 1014 Cell 128, March 9, 2007 ©2007 Elsevier Inc. DOI 10.1016/j.cell.2007.02.021 SnapShot: Actin Regulators II Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA REFERENCES Berg, J.S., Powell, B.C., and Cheney, R.E.
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  • Annexina7 and CAP1 Are Associated with Regulating Tumor Cell Adhesion Molecule Expression and Biological Behavior

    Annexina7 and CAP1 Are Associated with Regulating Tumor Cell Adhesion Molecule Expression and Biological Behavior

    AnnexinA7 and CAP1 are associated with regulating tumor cell adhesion molecule expression and biological behavior Type Research paper Keywords hepatocellular carcinoma, adhesion molecule, AnnexinA7, CAP1, Biological behaviors Abstract Introduction To find out the correlations between the effects of down-regulating AnnexinA7 and CAP1 gene on cell adhesion factors and the biological behavior of Hca-p cells cells, and finally infer the relationship between the two genes. Material and methods Western blot ,qRT-PCR,immunocytochemistry, CCK8 cell proliferation, flow cytometry, lymph node adhesion and transwell chamber assay testing . Results AnnexinA7 and CAP1 were consistent with the regulation of the expression of the adhesion molecules such as FAK, Src, Paxillin and E-cadherin. At the mRNA and protein levels, the expression of FAK, Src and Paxillin were increased with down-regulated AnnexinA7 and CAP1 genes, while E-cadherin was down-regulated in the change of these two genes. And the low expression of AnnexinA7 could affect the expression of CAP1 in mRNA and protein levels. otherwise, the localization of AnnexinA7 and CAP1 in hepatocellular carcinoma cells was also the same. After down-regulating the expression of CAP1, the functions of proliferation, lymph node adhesion and invasion were increased and earlyPreprint apoptotic ability was decreased in Hca-P cells. Conclusions AnnexinA7 and CAP1 could control the expression of these adhesion molecules in the same trend. We speculated they may be co-localization. And AnnexinA7 gene may be related to the molecular mechanism of CAP1 gene, which is likely to have a consistent effect on cell adhesion molecules and be closely related to the biological behaviors of Hca-P cells.AnnexinA7 and CAP1 may play an inhibitory role in lymph node metastasis to provide a reliable basis for the early identification of lymphatic metastasis in hepatocellular carcinoma.
  • Impaired Immune Surveillance Accelerates Accumulation of Senescent Cells and Aging

    Impaired Immune Surveillance Accelerates Accumulation of Senescent Cells and Aging

    ARTICLE https://doi.org/10.1038/s41467-018-07825-3 OPEN Impaired immune surveillance accelerates accumulation of senescent cells and aging Yossi Ovadya1, Tomer Landsberger2, Hanna Leins3,4, Ezra Vadai1, Hilah Gal1, Anat Biran1, Reut Yosef1, Adi Sagiv1, Amit Agrawal1, Alon Shapira1, Joseph Windheim1, Michael Tsoory5, Reinhold Schirmbeck4, Ido Amit 2, Hartmut Geiger3,6 & Valery Krizhanovsky 1 Cellular senescence is a stress response that imposes stable cell-cycle arrest in damaged 1234567890():,; cells, preventing their propagation in tissues. However, senescent cells accumulate in tissues in advanced age, where they might promote tissue degeneration and malignant transfor- mation. The extent of immune-system involvement in regulating age-related accumulation of senescent cells, and its consequences, are unknown. Here we show that Prf1−/− mice with impaired cell cytotoxicity exhibit both higher senescent-cell tissue burden and chronic inflammation. They suffer from multiple age-related disorders and lower survival. Strikingly, pharmacological elimination of senescent-cells by ABT-737 partially alleviates accelerated aging phenotype in these mice. In LMNA+/G609G progeroid mice, impaired cell cytotoxicity further promotes senescent-cell accumulation and shortens lifespan. ABT-737 administration during the second half of life of these progeroid mice abrogates senescence signature and increases median survival. Our findings shed new light on mechanisms governing senescent- cell presence in aging, and could motivate new strategies for regenerative medicine. 1 Department of Molecular Cell Biology, The Weizmann Institute of Science, 76100 Rehovot, Israel. 2 Department of Immunology, The Weizmann Institute of Science, 76100 Rehovot, Israel. 3 Institute of Molecular Medicine, Stem Cell and Aging, Ulm University, Ulm 89081, Germany.
  • Keratins and Plakin Family Cytolinker Proteins Control the Length Of

    Keratins and Plakin Family Cytolinker Proteins Control the Length Of

    RESEARCH ARTICLE Keratins and plakin family cytolinker proteins control the length of epithelial microridge protrusions Yasuko Inaba*, Vasudha Chauhan, Aaron Paul van Loon, Lamia Saiyara Choudhury, Alvaro Sagasti* Molecular, Cell and Developmental Biology Department and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, United States Abstract Actin filaments and microtubules create diverse cellular protrusions, but intermediate filaments, the strongest and most stable cytoskeletal elements, are not known to directly participate in the formation of protrusions. Here we show that keratin intermediate filaments directly regulate the morphogenesis of microridges, elongated protrusions arranged in elaborate maze-like patterns on the surface of mucosal epithelial cells. We found that microridges on zebrafish skin cells contained both actin and keratin filaments. Keratin filaments stabilized microridges, and overexpressing keratins lengthened them. Envoplakin and periplakin, plakin family cytolinkers that bind F-actin and keratins, localized to microridges, and were required for their morphogenesis. Strikingly, plakin protein levels directly dictate microridge length. An actin-binding domain of periplakin was required to initiate microridge morphogenesis, whereas periplakin-keratin binding was required to elongate microridges. These findings separate microridge morphogenesis into distinct steps, expand our understanding of intermediate filament functions, and identify microridges as protrusions that integrate actin and intermediate filaments. *For correspondence: [email protected] (YI); Introduction [email protected] (AS) Cytoskeletal filaments are scaffolds for membrane protrusions that create a vast diversity of cell shapes. The three major classes of cytoskeletal elements—microtubules, actin filaments, and inter- Competing interests: The mediate filaments (IFs)—each have distinct mechanical and biochemical properties and associate authors declare that no with different regulatory proteins, suiting them to different functions.