Snapshot: Actin Regulators I Anosha D

Home , Annexin A1, Calponin 1, CAP1, Espin (protein), MACF1, Spectrin

SnapShot: Actin Regulators I Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA

Representative Proteins Protein Family Biochemical Function H. sapiens D. melanogaster C. elegans A. thaliana S. cerevisiae Actin Nucleators

Formins associates with barbed ends, *hDia1, 2, 3, FHOD1, Diaphanous/Dia, Dia/CYK-1, DAAM, AtFH1-4, 5, 6-21 Bni1, Bnr1 promotes nucleation 2, 3, 4, DAAM1, 2, Cappucino/FMN, FRL, FHOD, INF FMN1, 2, FRL1, 2, 3, DAAM, FRL, FHOD, INF1, 2, Delphilin INF

Spir nucleates unbranched fi laments Spir-1, Spir-2 Spir/spire

Arp2/3 complex polymerizes Y-branched fi lament ARP2, ARP3, ARP2, ARP3, ARPC1, ARX1-7 ARP2, ARP3, ARPC1-5 Arp2, Arp3, Arc40, networks ARPC1A, 1B, 2, 3, 2, 3A, 3B, 4, 5, 5L 35, 18, 19, 15 4, 5, 5L

CARMIL links capping protein, Arp2/3 and CARMIL DCarmil †Q21301 myosin I

Coronin binds F-actin and Arp2/3 complex, coronin 1A, B, C; Coro/Coronin, Dpod1 COR-1, POD-1 Crn1 enhances actin depolymerization 2A, B; 7

Cortactin activates/modulates Arp2/3 complex, cortactin, HS1 DCortactin binds F-actin

SCAR/WAVE activates Arp2/3 complex SCAR1, SCAR2, SCAR WVE-1 AtSCAR 1, 2, 3, 4 family SCAR3

Arp2/3 complex regulators WASP family activates Arp2/3 complex *WASP, N-WASP WASp WSP-1 Las17/Bee1 G-Actin Binding Proteins

CAP/Srv2 recycles actin monomers CAP1, CAP2 Act up/Capulet CAS-1 AtCAP1 Srv2

Proﬁ lin promotes actin monomer addition at profi lin 1, 2, 3, 4 Chickadee PFN1, 2, 3 PRF1-3 (vegetative), Pfy1 barbed ends PRF4-5 (reproductive)

Thymosin β4 sequesters G-actin thymosin β4 Ciboulot TTH-1

Verprolin/WIP binds actin monomers, WASP WIP, WIRE/WICH, †Q8MLU0 WIP-1 Vrp1 CR16 F-Actin Binding Proteins Identiﬁ ed Cellular Role: X Membrane traffi cking and phagocytosis Ena/VASP family antagonizes CapZ capping at fi lament Mena, VASP, EVL Enabled UNC-34 X Cytokinesis ends, recruits profi lin X Cell surface organization and dynamics MIM binds both G- and F-actin, may MIM X Cell adhesion regulate nucleation-promoting factors X Multiple functions Pointed End Capping Proteins X Other/unknown Tropomodulin/ caps pointed ends, binds tropomyosin Tmod1, 2, 3, 4, Sanpodo TMD-1, TMD-2 †UNIPROT database accession number Tmod Lmod1, 2, 3 *Mutation linked to human disease Barbed End Capping Proteins

AIP1 promotes depolymerization by ADF/ AIP1/WDR1 Aip1 AIP-1/UNC-78 AtAIP1 Aip1 cofi lin

CapG caps barbed ends (calcium- capG CapG dependent)

CapZ/capping heterodimer caps barbed ends capZ (A1-3, B1-2) CapZ (cpa, cpb) CAP-1, CAP-2 AtCPA, AtCPB Cap1, Cap2 protein/β-actinin

Eps8 caps barbed ends, involved in Rac Eps8, Eps8L1, 2, 3 Arouser, †Q95TJ6 EPS-8 signalling Actin Depolymerizing/Severing Proteins

ADF/coﬁ lin promotes actin fi lament disassembly cofi lin1, cofi lin2, ADF Cofi lin/Twinstar UNC-60A, B AtADF1-12 Cof1

Gelsolin/villin severs actin fi laments gelsolin, adseverin/ Gelsolin, Villin-like/ gelsolin (†Q21253), VLN1-5 superfamily scinderin, villin, Quail, Flightless I supervillin advillin, supervillin, (†P91041), FLI-1 ﬂ ightless I

Twinﬁ lin inhibits actin nucleotide exchange, twinfi lin-1/A6, Twinfi lin †U46668 Twf1 severs actin twinfi lin-2 Actin Bundling/Crosslinking Proteins

α-actinin connects and organizes actin fi laments α-actinin1-4 α-actinin/Flightless A ATN-1

EPLIN stabilizes and bundles actin fi laments EPLIN

Espin forms parallel actin bundles *espin Forked

Fascin bundles actin fi laments fascin 1, 2, 3 Singed

Filamin orthogonally crosslinks actin fi laments fi lamin *A, *B, C Jitterbug, Cheerio †Q18109

Fimbrin/plastin bundles actin fi laments plastin 1-3 (I, L, T) Fimbrin †Q965Y6 AtFIM1 Sac6

See online version for references and abbreviations. Actin Regulators II will appear in the March 9 issue of Cell. 626 Cell 128, February 9, 2007 ©2007 Elsevier Inc. DOI 10.1016/j.cell.2007.02.001 SnapShot: Actin Regulators I Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA

References

Aspenstrom, P. (2005). The verprolin family of proteins: Regulators of cell morphogenesis and endocytosis. FEBS Lett. 579, 5253–5259.

Daly, R.J. (2004). Cortactin signalling and dynamic actin networks. Biochem. J. 382, 13–25. dos Remedios, C.G., Chhabra, D., Kekic, M., Dedova, I.V., Tsubakihara, M., Berry, D.A., and Nosworthy, N.J. (2003). Actin binding proteins: regulation of cytoskeletal microfilaments. Physiol. Rev. 83, 433–473.

Goley, E.D., and Welch, M.D. (2006). The ARP2/3 complex: An actin nucleator comes of age. Nat. Rev. Mol. Cell Biol. 7, 713–726.

Higgs, H.N., and Peterson, K.J. (2005). Phylogenetic analysis of the formin homology 2 domain. Mol. Biol. Cell 16, 1–13.

Kerkhoff, E. (2006). Cellular functions of the Spir actin-nucleation factors. Trends Cell Biol. 16, 477–483.

Krause, M., Dent, E.W., Bear, J.E., Loureiro, J.J., and Gertler, F.B. (2003). Ena/VASP proteins: Regulators of the actin cytoskeleton and cell migration. Annu. Rev. Cell Dev. Biol. 19, 541–564.

Paavilainen, V.O., Bertling, E., Falck, S., and Lappalainen, P. (2004). Regulation of cytoskeletal dynamics by actin-monomer-binding proteins. Trends Cell Biol. 14, 386–394.

Silacci, P., Mazzolai, L., Gauci, C., Stergiopulos, N., Yin, H.L., and Hayoz, D. (2004). Gelsolin superfamily proteins: Key regulators of cellular functions. Cell. Mol. Life Sci. 61, 2614–2623.

Uetrecht, A.C., and Bear, J.E. (2006). Coronins: The return of the crown. Trends Cell Biol. 16, 421–426.

Abbreviations

ADF: actin-depolymerizing factor AIP1: actin-interacting protein 1 ARP: actin-related protein ARPC: actin-related protein 2/3 complex CAP1 and CAP2: cyclase-associated protein 1 and 2 CARMIL: capping protein, Arp2/3, and myosin linker DAAM: dishevelled-associated activator of morphogenesis Ena: Enabled EPLIN: epithelial protein lost in neoplasm Eps8: EGF receptor pathway substrate 8 EVL: Ena/VASP-like protein FHOD: formin homology-2 domain-containing protein FMN: formin FRL: formin-related gene in leukocytes hDia: human homolog of Drosophila Diaphanous INF: inverted formin Lmod: leiomodin Mena: mammalian enabled MIM: missing in metastasis N-WASP: neuronal Wiskott-Aldrich Syndrome protein SCAR/WAVE: suppressor of cAMP receptor/WASP family verprolin-homologous protein Tmod: tropomodulin VASP: vasodilator-stimulated phosphoprotein WASP: Wiskott-Aldrich Syndrome protein WIP: WASP-interacting protein WIRE/WICH: WIP-related/WIP and CR16 homologous protein

626.e1 Cell 128, February 9, 2007 ©2007 Elsevier Inc. DOI 10.1016/j.cell.2007.02.001 SnapShot: Actin Regulators II Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA

Representative Proteins Protein Family H. sapiens D. melanogaster C. elegans A. thaliana S. cerevisiae Endocytosis and Exocytosis

ABP1/drebrin mABP1, drebrin, drebrin- †Q95RN0 †Q9XUT0 Abp1 like

EPS15 EPS15 Eps-15 EHS-1 †Q56WL2 Pan1

HIP1R HIP1R †Q8MQK1 †O62142 Sla2

Synapsin synapsin Ia, Ib, IIa, IIb, III Synapsin SNN-1 Plasma Membrane Association

Anillin anillin Scraps ANI-1, 2, 3

Annexins annexin A1–11, 13 (actin Annexin B9-11 NEX-1–4 ANN1-8 binding: 1, 2, 6)

ERM proteins ezrin, radixin, moesin DMoesin ERM-1

MARCKS MARCKS, MRP/ Akap200 MACMARCKS/F52

Merlin *merlin/NF2 Merlin NFM-1

Protein 4.1 4.1R, G, N, B Coracle

Spectrin α-spectrin (1–2), β-spectrin α-spectrin, β-spectrin, β heavy- SPC-1 (α-spectrin), UNC-70 (1–4), β heavy-spectrin/ spectrin/Karst (β-spectrin), SMA-1 (β heavy- karst spectrin) Identiﬁ ed Cellular Role: X Membrane traffi cking and phagocytosis Cell-Cell Junctions X Cytokinesis α-catenin α-catenin 1–3 α-catenin HMP-1 X Cell surface organization and dynamics X Cell adhesion Afadin afadin/AF6 Canoe AFD-1 X Multiple functions ZO-1 ZO-1, ZO-2, ZO-3 ZO-1/Polychaetoid †Q56VX4 X Other/unknown

Cell-Extracellular Matrix Junctions †UNIPROT database accession number *Mutation linked to human disease Dystrophin/utrophin *dystrophin, utrophin/ Dystrophin DYS-1 DRP1, DRP2

LASP LASP-1, LASP-2, LIM- Lasp †P34416 nebulette

Palladin palladin

Parvin α-, β-, χ-parvin †Q9VWD0 PAT-6 (α-parvin)

Plakins BPAG1, MACF1, plectin Shortstop/Kakapo VAB-10 †Q8S8J6, Q38843, Q9C7V7

Talin talin 1–2 Rhea/Tendrils †Q22860

Tensin tensin 1–3 Blistery/Tensin †P92160

Vinculin vinculin Vinculin DEB-1

Zyxin/paxillin zyxin, paxillin Zyx102, Dpax ZYX-1, paxillin (†Q09476) Motor Proteins and Regulators

Caldesmon caldesmon Crtp

Calponin calponin 1–3 Chd64 CPN-1–4 Scp1

Myosin family Myosin types 1, 2, 3, *5, *6, Myosin types 1, 2, 3, 5, 6, 7, Myosin types 1, 2, 5, 6, 7, 9, 12 Myosin types 8, 11 Myosin types 1, 2, 5 *7, 9, 10, *15, 16, 18, 19 15, 18, 20

Tropomyosin tropomyosin 1, *2, *3, 4 Tm1, Tm2 LEV-11/TMY-1 †Q8LE98 Tpm1, Tpm2

Troponin troponin T*1, *2, 3; C1, 2; Troponin T/Upheld, Troponin TNI-1-4, TNC-1-2, TNT-1-4 I1, 2, *3 I/Wings up, Troponin C1-3, Tina-1 Muscle Components

Myopalladin myopalladin, *myotilin

Nebulin *nebulin, *nebulette, N-RAP Nebulin †P34416

Titin/connectin *titin/connectin D-titin, Projectin/Twitchin Ce-titin, stretchin Nuclear Actin-Associated Proteins

Chromatin-remodeling BAF, PBAF, TIP60, p400 BAP Ino80, NuA4 complexes

hnRNP proteins DBP40 (R. norvegicus) hrp36, hrp65 (C. tentans)

Nuclear DNA Helicase II NDHII Maleless/MLE RHA-1

See online version for references and abbreviations. Actin Regulators I appeared in the February 9 issue of Cell. 1014 Cell 128, March 9, 2007 ©2007 Elsevier Inc. DOI 10.1016/j.cell.2007.02.021 SnapShot: Actin Regulators II Anosha D. Siripala and Matthew D. Welch Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA

References

Berg, J.S., Powell, B.C., and Cheney, R.E. (2001). A millennial myosin census. Mol. Biol. Cell 12, 780–794.

Bettinger, B.T., Gilbert, D.M., and Amberg, D.C. (2004). Actin up in the nucleus. Nat. Rev. Mol. Cell Biol. 5, 410–415.

Bretscher, A., Edwards, K., and Fehon, R.G. (2002). ERM proteins and merlin: Integrators at the cell cortex. Nat. Rev. Mol. Cell Biol. 3, 586–599.

Calderwood, D.A., Shattil, S.J., and Ginsberg, M.H. (2000). Integrins and actin filaments: Reciprocal regulation of cell adhesion and signaling. J. Biol. Chem. 275, 22607–22610.

Clark, K.A., McElhinny, A.S., Beckerle, M.C., and Gregorio, C.C. (2002). Striated muscle cytoarchitecture: An intricate web of form and function. Annu. Rev. Cell Dev. Biol. 18, 637–706.

Critchley, D.R. (2004). Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 32, 831–836.

Goodson, H.V., and Dawson, S.C. (2006). Multiplying myosins. Proc. Natl. Acad. Sci. USA 103, 3498–3499.

Hayes, M.J., Rescher, U., Gerke, V., and Moss, S.E. (2004). Annexin-actin interactions. Traffic 5, 571–576.

Kaksonen, M., Toret, C.P., and Drubin, D.G. (2006). Harnessing actin dynamics for clathrin-mediated endocytosis. Nat. Rev. Mol. Cell Biol. 7, 404–414.

Lo, S.H. (2006). Focal adhesions: What’s new inside. Dev. Biol. 294, 280–291.

Abbreviations

ABP1: actin-binding protein 1 Akap200: A kinase anchor protein 200 BAF: BRG1-associated factor BAP: Brahma-associated protein BIN2: bridging integrator 2 BPAG1: bullous pemphigoid antigen 1 Crtp: caldesmon-related protein DBP40: DNA-binding protein 40 DRP1: dystrophin-related protein 1 DRP2: dystrophin-related protein 2 EPS15: epidermal growth factor receptor pathway substrate 15 HIP1R: huntingtin-interacting protein 1 related hrp: heterogeneous nuclear ribonucleoprotein LASP: Lim and SH3 protein MACF1: microtubule-actin crosslinking factor 1 MARCKS: myristoylated alanine-rich protein kinase C substrate MRP/MACMARCKS: MARCKS-related protein/macrophage MARCKS NDHII: nuclear DNA helicase II N-RAP: nebulin-related anchoring protein NF2: neurofibromin 2 NuA4: nucleosome acetyltransferase of H4 PBAF: polybromo-associated BRG1 associated factor TIP60: HIV-1 Tat-interacting protein of 60 kilodaltons ZO: zonula occludens