Of Selected Cold-Blooded Vertebrates – Systematics, Taxonomy, Morphology

MASARYK UNIVERSITY FACULTY OF SCIENCE DEPARTMENT OF BOTANY AND ZOOLOGY

PARASITIC NEMATODES (N EMATODA ) OF SELECTED COLD -BLOODED VERTEBRATES – SYSTEMATICS , TAXONOMY , MORPHOLOGY

ŠÁRKA MAŠOVÁ

SUMMARY OF PH.D. T HESIS

BRNO , 2014

PH.D. THESIS: Mašová Š, 2013: Parasitic nematodes (Nematoda) of selected cold-blooded vertebrates – systematics, taxonomy, morphology. Ph.D. Thesis, in English – 215 pp., Faculty of Science, Department of Botany and Zoology, Masaryk University, Brno, Czech Republic.

SUPERVISOR : prof. Ing. Vlastimil Baruš, DrSc. Department of Botany and Zoology, Faculty of Science Masaryk University, Brno, Czech Republic

REVIEWERS : Dr. David González-Solís El Colegio de la Frontera Sur Unidad Chetumal Chetumal, Quintana Roo, Mexico

RNDr. Marta Špakulová, DrSc. Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia

rd PH.D. THESIS DEFENCE : 23 July 2014, 10 a.m., Large meeting room 432, Pavilion A17, University Campus Bohunice, Masaryk University, Kamenice 5, 625 00 Brno - Bohunice, Czech Republic.

CONTENTS

1. INTRODUCTION...... 3 2. AIMS OF THE THESIS ...... 4 3. STRUCTURE OF THE THESIS...... 4 4. SUMMARY OF RESULTS...... 5 5. CONCLUSIONS...... 8 6. REFERENCES...... 8 7. CURRICULUM VITAE ...... 9

1. INTRODUCTION Nematoda commonly named roundworms, eelworms or threadworms (from the Greek word for thread -nema = νήα ) are one of the most widespread groups of animals (SCHMIDT -RHAESA 2014). They are recognized as the second largest phylum. They are triploblastic, unsegmented worms without a true coelom (KENT 2004). They possess a pseudocoelom. Nematoda is an ancient group of organisms, demonstrating true biological progress, manifested by a high level of taxonomic diversification, intensive speciation, diversity of life cycles and life strategies, trophic specialization and the occupation of various habitats (WEBSTER et al. 2008). ZHANG (2011) mentions three classes, three subclasses, 31 orders, 267 families, and 2829 genera of nematodes. Up to now, 25,043 species (including 10 fossil species) have been described according to ZHANG (2013), who updated data using Thomson Reuters' Zoological Record ® database (Fig. 1). In April 2014 the total number of described nematode species was 26,567 according to Thomson Reuters' Index to organism names . SCHMIDT -RHAESA (2014) states, that about 27,000 species are currently described, but this number is only a fraction of the real nematode diversity. ANDERSON (2000) estimates at least 40,000 species else further exist. About 33% of all the nematode genera which have been described occur as parasites of vertebrates (ANDERSON 1984). Cold-blooded vertebrates are hosts of very interesting and important nematode parasites. They can infect any part of the host body, including the body cavity, most of the internal organs, deep layers of the skin or fins, and external muscle layers as adults or as larvae (MOLNÁR et al . 2006, YANONG 2002, MORAVEC 2013). Nematodes may cause all sorts of health problems and economic losses (losses in cultures and breedings) (MORAVEC 2013). Nematodes occurring in reptiles and fishes have often indirect life cycle, they utilize intermediate and/or paratenic hosts in transmission (ANDERSON 2000). The ancestors of fish nematode parasites originated in early terrestrial vertebrates and transferred later to fish after they had acquired heteroxeny and paratenesis (ANDERSON 1984, 1988, 2000). The discovery of phylogenetic relationships or occasionally species determination, is possible through comparison of different taxa from the aspect of "integrative taxonomy". This brings approaches of study from multiple and complementary perspectives, utilising various methodologies like e. g. classical morphology and molecular biology (THOMAS et al . 1997, FONSECA et al . 2008). I have studied selected nematode species from Africa (Senegal, Kenya and Sudan). This material utilized, was collected by me or my colleagues. I have paid attention primarily to the nematode morphology and increasing the knowledge of their diversity in Africa. The present thesis has two main topics: nematodes of fish and reptiles from the viewpoint of morphological analyses and detailed descriptions. It is divided into ten chapter including two appendices. The "Summary of results" chapter is a collection of publications relating to the thesis topics.

3 2. AIMS OF THE THESIS The general aim of this thesis was to investigate parasitic nematodes of cold-blooded vertebrates from the Afrotropical realm. Thesis sub-aims: • to study and describe in detail the morphology of selected taxa using various methods of light and scanning electron microscopy • to differentiate and characterise selected nematodes using molecular techniques • to add to the knowledge of the morphometric variability of selected taxa • to make detailed redescriptions of selected nematode morphospecies • to identify features with species value and compile determination keys of selected nematodes

3. STRUCTURE OF THE THESIS The thesis is presented as a set of seven manuscripts (Papers I–VII), six published and one submitted to a peer review in scientific journals:

Paper I Moravec František, Jirk ů Miloslav, Charo-Karisa Harrison, Mašová Šárka (2009): Mexiconema africanum sp. n. (Nematoda: Daniconematidae) from the catfish Auchenoglanis occidentalis from Lake Turkana, Kenya. Parasitology Research , 105, 1047-1052. Paper II Mašová, Šárka , Moravec, František, Baruš, Vlastimil, Seifertová, Mária (2010): Redescription, systematic status and molecular characterization of Multicaecum heterotis Petter, Vassiliadès et Marchand, 1979 (Nematoda: Heterocheilidae), an intestinal parasite of Heterotis niloticus (Osteichthyes: Arapaimidae) in Africa. Folia Parasitologica , 57, 280-288. Paper III Mašová, Šárka (2012): Structure of the cephalic end and eggs of female Cithariniella khalili Petter, Vassiliadès et Troncy, 1972 (Nematoda: Pharyngodonidae), a parasite of African fishes . Helminthologia , 49, 115-118. Paper IV Mašová Šárka , Baruš Vlastimil, Moravec František (2011): New morphological data on the first-stage larvae of two Procamallanus species (Nematoda: Camallanidae) based on SEM studies. Folia parasitologica , 58, 318-321. Paper V Mašová Šárka , Baruš Vlastimil, Hodová Iveta, Mat ějusová Iveta, Koubek Petr, Koubková Božena (2008): Morphometric and molecular characterization of Parapharyngodon echinatus (Nematoda, Pharyngodonidae) from the Senegal gecko ( Tarentola parvicarinata ). Acta Parasitologica , 53, 274-283. Paper VI Mašová Šárka , Baruš Vlastimil, Hodová Iveta, Koubek Petr, Koubková Božena (2009): Redescription of Parapharyngodon micipsae (Seurat 1917) (Nematoda Pharyngodonidae) from the new host Tarentola parvicarinata Joger 1980 (Squamata Gekkonidae). Tropical Zoology , 22, 243-255.

Paper VII Mašová Šárka , Baruš Vlastimil, Seifertová Mária, Malala John, Jirk ů Miloslav (2014): Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note to D. dujardini (Nematoda: Heterocheilidae), parasites of the Crocodylus niloticus . Submitted to Zootaxa .

4. SUMMARY OF RESULTS Paper I Moravec František, Jirk ů Miloslav, Charo-Karisa Harrison, Mašová Šárka (2009): Mexiconema africanum sp. n. (Nematoda: Daniconematidae) from the catfish Auchenoglanis occidentalis from Lake Turkana, Kenya. Parasitology Research , 105, 1047–1052.

A new species of dracunculoid nematode, Mexiconema africanum sp. n. (Daniconematidae), was described from the abdominal cavity and the intestine (rarely also the gall bladder) of the catfish Auchenoglanis occidentalis (Valenciennes , 1840) (Claroteidae, Siluriformes) from Lake Turkana, Kenya. The new species differs from two other congeners mainly in the absence of two large cell nuclei in the glandular oesophagus, presence of well developed lateral cephalic elevations, more numerous (14) cephalic papillae and a much longer body in gravid females (18– 22 mm); from Mexiconema cichlasomae Moravec, Vidal and Salgado Maldonado, 1992 also has less numerous (two) caudal processes and a different arrangement of genital papillae in the male. Mexiconema africanum is the first representative of the dracunculoid family Daniconematidae described from Africa.

Paper II Mašová, Šárka, Moravec, František, Baruš, Vlastimil, Seifertová, Mária (2010): Redescription, systematic status and molecular characterization of Multicaecum heterotis Petter, Vassiliadès et Marchand, 1979 (Nematoda: Heterocheilidae), an intestinal parasite of Heterotis niloticus (Osteichthyes: Arapaimidae) in Africa. Folia Parasitologica, 57, 280–288.

Ascaridoid nematodes referable as Brevimulticaecum heterotis (Petter, Vassiliadès et Marchand, 1979) Khalil, 1984 were recorded from the intestine of the African bonytongue, Heterotis niloticus (Cuvier) (Arapaimidae, Osteoglossiformes), from the Mare Simenti in the Niokolo Koba National Park, East Senegal and from Kostí, Sudan. Their examination using, light microscopy and for the first time both environmental scanning electron microscopy and scanning electron microscopy, revealed some previously unreported morphological features and made possible a detailed redescription of this species. The most important finding is the presence of dentigerous ridges on the inner edges of the lips, which confirms the attribution of this species to Multicaecum Baylis, 1923, where it was originally placed, and not to Brevimulticaecum Mozgovoy in Skryabin, Shikhobalova et Mozgovoy, 1951 where it had subsequently been transferred. Multicaecum heterotis is the first species of the genus to be sequenced. Partial sequences of the small ribosomal subunit (18S) and internal transcribed spacer 2 region (ITS2) of nuclear ribosomal DNA (rDNA) have been analysed and compared with other nematode species. The sequences obtained of the partial 18S rDNA were 854 bp in length and did not show

5 intraspecific nucleotide variability. The similarity between the partial 18S sequence of M. heterotis and other nematode sequences included in the alignment produced in BLAST varies from 92% to 98%. The sequences obtained from ITS2 region were 418 bp length. The resulting ITS2 sequence showed 88–100% homology, but only 8–27% coverage in relation to previously published nematode sequences using BLAST. A key to Brevimulticaecum and Multicaecum species is provided in the end.

Paper III Mašová, Šárka (2012): Structure of the cephalic end and eggs of female Cithariniella khalili Petter, Vassiliadès et Troncy, 1972 (Nematoda: Pharyngodonidae), a parasite of African fishes. Helminthologia , 49, 115–118.

Scanning electron microscopy was used to study the female surface morphological characteristics of the mouth opening (lips and such adjacent structures as amphids, papillae and chitinous ribbed flaps), caudal area (vulva, anus), and eggs with filaments on poles of the pharyngodonid species Cithariniella khalili Petter, Vassiliadès et Troncy, 1972, obtained from Synodontis frontosa Vaillant at Lake Turkana in north-western Kenya. Main scientific contribution is a more accurate documentation of the chitinous ribbed flaps in the buccal cavity and of the eggs structures. It was presumed that new filaments should be created during ripening of eggs. This was supported by the existence of buds on the poles of some eggs. The reported observations have clarified morphological details of C. khalili that were difficult to observe by light microscopy only. Knowledge of such morphological characters, as presence of oral lamellae, may be important in hypothesizing phylogenetic relationships among the Pharyngodonidae.

Paper IV Mašová Šárka, Baruš Vlastimil, Moravec František (2011): New morphological data on the first-stage larvae of two Procamallanus species (Nematoda: Camallanidae) based on SEM studies. Folia parasitologica , 58, 318–321.

First-stage larvae of camallanid nematodes Procamallanus (Procamallanus ) laeviconchus (Wedl, 1862) and Procamallanus (Procamallanus ) sp. from naturally infected Distichodus niloticus (Hasselquist) and Clarias gariepinus (Burchell), respectively, from Lake Turkana, Kenya (new geographical records) are described, studied for the first time by scanning electron microscopy. Larvae of both species are characterised by the presence of a dorsal cephalic tooth, four submedian cephalic papillae and a pair of amphids, and by the elongate tail with several terminal digit-like processes. The latter formations probably serve for the attachment of larvae to the substrate in water when the larvae attract copepod intermediate hosts by their movements; these structures, especially their numbers, may be of taxonomic importance in camallanid nematodes.

6 Paper V Mašová Šárka, Baruš Vlastimil, Hodová Iveta, Mat ějusová Iveta, Koubek Petr, Koubková Božena (2008): Morphometric and molecular characterization of Parapharyngodon echinatus (Nematoda, Pharyngodonidae) from the Senegal gecko ( Tarentola parvicarinata ). Acta Parasitologica , 53, 274–283.

A West African species of gecko, Tarentola parvicarinata Joger (Gekkonidae), collected in the Niokolo Koba National Park in East Senegal is a newly discovered host species for the nematode Parapharyngodon echinatus (Rudolphi, 1819). Fifty one specimens of P. echinatus were studied under a light microscope and thirteen specimens were studied for the first time by a scanning electron microscope. The main differences between P. echinatus and other African species, especially Parapharyngodon micipsae (Seurat, 1917), were in the shape of annules on the body cuticle, broad lateral alae and their terminations, the shape of the distal extremity of the spicule and the number of outgrowths at the anterior cloacal lip. For the purposes of DNA characterization, partial sequence of the small subunit ribosomal RNA gene (SSU rRNA) was obtained from two specimens of P. echinatus . This is the first published sequence of a species from the family Pharyngodonidae.

Paper VI Mašová Šárka, Baruš Vlastimil, Hodová Iveta, Koubek Petr, Koubková Božena (2009): Redescription of Parapharyngodon micipsae (Seurat 1917) (Nematoda Pharyngodonidae) from the new host Tarentola parvicarinata Joger 1980 (Squamata Gekkonidae). Tropical Zoology , 22, 243–255.

This study, based on a morphometric approach for the identification of the nematode parasite Parapharyngodon micipsae (Seurat 1917), reports the first record of P. micipsae in a West African species of gecko, Tarentola parvicarinata Joger, 1980 (Gekkonidae), collected in the Niokolo Koba National Park, Senegal. Specimens of P. micipsae were examined under a light microscope and for the first time with a scanning electron microscope. The main differences between males of P. micipsae and similar African species [ P. margaritiferi Hering- Hagenbeck, 2001, P. kenyaensis Bursey et Goldberg, 2005, P. meridionalis (Chabaud et Golvan, 1962)] are in the shape and length of spicule, number and distribution of caudal papillae, outgrowths morphology, cloaca-tail distance and host. In the case of females, P. micipsae differs significantly from similar species mainly in oesophagus length, anus-tail end distance and egg size. Morphological differences between P. micipsae and P. echinatus (Rudolphi, 1819) males in are in the lateral alae, the form of distal end of spicule and the morphology of outgrowths were confirmed as stable features.

Paper VII Mašová Šárka, Baruš Vlastimil, Seifertová Mária, Malala John, Jirk ů Miloslav (2014): Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note to D. dujardini (Nematoda: Heterocheilidae), parasites of the Crocodylus niloticus . Submitted to Zootaxa .

An examination of one specimen of Nile crocodile, Crocodylus niloticus (Laurenti, 1768), from the Lake Turkana (Kenya), revealed the presence of two ascaridoid nematodes referable to genus Dujardinascaris Baylis, 1947. Dujardinascaris madagascariensis Chabaud et Caballero, 1966 are studied by scanning electron microscopy, redescribed, and differentiated from D. dujardini

7 (Travassos, 1920), which has a lower intensity in mixed infection. This species is the second of the genus Dujardinascaris to be sequenced. An internal fragment of the small ribosomal subunit (18S) and nuclear ribosomal DNA internal transcribed spacer region (ITS2) were amplified. Additionally, the slowly evolving 18S gene region was used for phylogenetic analysis. Molecular data was used to confirm the morphological similarity and determine that D. madagascariensis shares the closest relationship with D. waltoni , as well as with other species of the family Heterocheilidae. A key to species of Dujardinascaris is provided as well.

5. CONCLUSIONS

From the analyses conducted in this thesis, the following conclusions can be drawn:

• The morphologic and morphometric studies of several nematode species have been done. The results are redescriptions of nematode characters from two considerable groups of vertebrates (fishes and reptiles). Three papers moreover contain the partial sequences for the taxonomic identification of species Dujardinascaris madagascariensis (18S, ITS2), Parapharyngodon echinatus (18S) and Multicaecum heterotis (18S, ITS2). • The differences found in the morphology of one parasite provided the sufficient data for the new species Mexiconema africanum description. • A modification of SEM – environmental scanning electron microscopy (ESEM) was trialled, to attempt to identify a new way of viewing nematodes. This method was quite useful when applied to the species Multicaecum heterotis . • Using sample tilting (common approach employed in SEM stereomicroscopy) was possible to obtain a SEM three-dimensional images of Dujardinascaris madagascariensis .

6. REFERENCES

ANDERSON , R. C. 1984. The origins of zooparasitic nematodes. Canadian Journal of Zoology - Revue Canadienne de Zoologie, 62, 317–328. ANDERSON , R. C. 1988. Nematode transmission patterns. Journal of Parasitology, 74, 30–45. ANDERSON , R. C. 2000. Nematode parasites of vertebrates. Their development and transmission, Oxon, Cabi Publishing. FONSECA , G., D ERYCKE , S. & M OENS , T. 2008. Integrative taxonomy in two free-living nematode species complexes. Biological Journal of the Linnean Society, 94, 737–753. KENT , M. 2004. Advanced Biology, UK, Oxford University Press. MOLNÁR , K., B UCHMANN , K. & S ZÉKELY , C. 2006. Fish Diseases and Disorders: Protozoan and Metazoan nd Infections. In: WOO , P. T. K. (ed.) Fish Diseases and Disorders. 2 ed. Oxfordshire, UK, CABI. MORAVEC , F. 2013. Parasitic Nematodes of Freshwater Fishes of Europe, Prague, Czech Republic, Academia. SCHMIDT -RHAESA , A. 2014. Handbook of Zoology. Gastrotricha, Cycloneuralia and Gnathifera: Nematoda, Berlin/Boston, Walter de Gruyter & Co. THOMAS , W. K., V IDA , J. T., F RISSE , L. M., M UNDO , M. & B ALDWIN , J. G. 1997. DNA sequences from formalin- fixed nematodes: Integrating molecular and morphological approaches to taxonomy. Journal of Nematology, 29, 250–254. WEBSTER , J. M., E RIKSSON , K. B. & M CNAMARA , D. G. 2008. An anecdotal history of nematology , Pensoft Publishers. ZHANG , Z. Q. 2011. Animal biodiversity: An outline of higher-level classification and survey of taxonomic richness. Zootaxa , 3148, 1–237. ZHANG , Z. Q. 2013. Animal biodiversity: An update of classification and diversity in 2013. Zootaxa, 3703, 5–11.

8 7. CURRICULUM VITAE

Šárka Mašová

Date and place of birth

September 29, 1983, Nové M ěsto na Morav ě, Czech Republic

Contacts

+420 549 493 289 | +420 776 644 413 | [email protected]

Education

2008 - present: combined Ph.D. study , Masaryk University, Faculty of Science, Department of Botany and Zoology, Parasitology (Thesis: Parasitic nematodes of selected cold-blooded vertebrates - systematics, taxonomy, morphology) 2012: RNDr. , Masaryk University, Faculty of Science, Department of Botany and Zoology, Parasitology [Thesis: Two new anoplocephalid (Cestoda) from Senegal reptiles (West Africa)] 2006 - 2008: Mgr. , Masaryk University, Faculty of Science, Department of Botany and Zoology, Zoology (Thesis: Nematodes parasitic in selected cold-blooded vertebrates of West Africa) 2003-2006: Bc. , Masaryk University, Faculty of Science, Department of Botany and Zoology, Systematic Biology and Ecology (Thesis: Nematodes of African freshwater fishes)

Working experience

08/2008 - present: specialist, Masaryk University, Department of Botany and Zoology 10/2009 - 2011: technician, Masaryk University, Department of Botany and Zoology

Teaching activity

2012, 2013: Optical contrast and imaging methods 2013-present: Diploma thesis tutorial (teachers of biology) 2012-2013: Bachelor thesis of biology (teachers of biology) 2009-2012: Zoology microtechnique (helping in several lessons) 2010: Histology and organology - practical course (helping in several lessons) 2008, 2009: General zoology - practical course

Specialised courses

14.-18. 10. 2013: Microscopy Methods in Biomedicine (Institute of Molecular Genetics AS CR, Prague) 10.-15. 6. 2013: Summer School focus on microinjection and advanced imaging (CEITEC & VUVEL, Brno)

9 3.-7. 6. 2013: Current Methods in Cell Biology (Charles University in Prague, 1st Faculty of Medicine) 18.-22.6. 2012: Biotechnology Course: III. Summer School of the molecular biology of nucleic acids and Genomics (Department of Animal Morphology, Physiology and Genetics, Mendel University Brno) 2011: Polar ecology course (Centre for Polar Ecology, Department of ecosystem biology, Faculty of Science, University of South Bohemia in České Bud ějovice) 12-16. 10. 2009: Autumn School on Basic Electron Microscopy (Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, Brno) 15-19. 6. 2009: Transmission electron microscopy in life sciences (Czechoslovak Microscopy Society, Prague) 23.2.-27. 3. 2009: Course: Biological preparations in electron microscopes. (Biology Centre of the Academy of Sciences of the Czech Republic, České Bud ějovice) 10-16. 1. 2009: Practical Course in Advanced Microscopy, module: Fine Structure Preparation in Electron Microscopy (University of Zürich, ETH Zürich; Switzerland)

Award

2008: Winner of the competition for two years scholarship for young scientific worker, who provide Czechoslovak Microscopy Society and FEI Company Czech Republic, s.r.o. for Study of structure of parasitic nematodes by electron microscopy.

Professional associations

Czechoslovak Microscopy Society, European Microscopy Association , Czech Society for Parasitology

Stays abroad

2014, 10 days Croatia, (Selce) field work 2014, 8 weeks Antarctica (Jamese Ross Island, J. G. Mendel station), field work 2013, 4 weeks Burundi (Tanganica), field work 2013, 3 weeks France (Marseille) field work 2013, 10 days Croatia, (Selce) field work 2012/2013, 9 weeks Antarctica (Jamese Ross Island, J. G. Mendel station), field work 2012, 3 weeks Zimbabwe (Lakes Kariba and Chivero), field work 2011/2012, 8 weeks Antarctica (Jamese Ross Island, J. G. Mendel station), field work 2011, 1 week France, National Museum of Natural History in Paris, laboratory work 2011, 2 weeks Norway (Svalbard), field work 2008, 6 weeks Kenya (Lake Turkana), field work

10 Publications

Mašová Šárka , Baruš Vlastimil, Seifertová Mária, Malala John & Jirk ů Miloslav 2014 (Submitted). Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note on D. dujardini (Nematoda: Heterocheilidae), parasites of Crocodylus niloticus . Submitted to Zootaxa . Mašová Šárka , Baruš Vlastimil (2013): Redescription of cystacanths of Corynosoma pseudohamanni Zdzitowiecki, 1984 (Acanthocephala: Polymorphidae) from paratenic fish hosts. Folia Parasitologica , 60, 169–176. Baruš Vlastimil, Mašová Šárka , Koubková Božena, Sitko Jiljí (2013): Subulura mackoi n. sp. (Nematoda: Subuluridae) and the zoogeography of subulurids parasitizing birds. Helminthologia , 50, 46–56. Mašová Šárka , Kašparová Eva (2012): Preliminary report on fish diversity at the Prince Gustav Channel, the northern part of the James Ross Island, Antarctica. Czech Polar Reports , 2, 92–102. Mašová Šárka (2012): Structure of the cephalic end and eggs of female Cithariniella khalili Petter, Vassiliadès et Troncy, 1972 (Nematoda: Pharyngodonidae), a parasite of African fishes. Helminthologia , 49, 115–118. Mašová Šárka , Tenora František, Baruš Vlastimil (2012): Oochoristica koubeki n. sp. (Cestoda, Anoplocephalidae) from African Chamaeleo senegalensis (Chamaeleonidae) and emendation of the genus Oochoristica Lühe, 1898. Helminthologia , 49, 27–32. Mašová Šárka , Baruš Vlastimil, Moravec František (2011): New morphological data on the first-stage larvae of two Procamallanus species (Nematoda: Camallanidae) based on SEM studies. Folia parasitologica , 58, 318–321. Foitová Ivona, Mašová Šárka , Tenora František, Hodová Iveta, Koubková Božena, Vysko čilová Martina, Baruš Vlastimil, Nurcahyo Wisnu (2011): Redescription and resurrection of Bertiella satyri (Cestoda, Anoplocephalidae) parasitizing the orangutan ( Pongo abelii ) in Indonesia. Parasitology Research , 109, 689–697. Mašová Šárka , Tenora František, Baruš Vlastimil, Koubek Petr (2010): A new anoplocephalid (Cestoda) from Tarentola parvicarinata (Lacertilia: Gekkonidae) in Senegal (West Africa). Journal of Parasitology , 96, 977–981. Mašová Šárka , Moravec František, Baruš Vlastimil, Seifertová Mária (2010): Redescription, systematic status and molecular characterization of Multicaecum heterotis Petter, Vassiliadès et Marchand, 1979 (Nematoda: Heterocheilidae), an intestinal parasite of Heterotis niloticus (Osteichthyes: Arapaimidae) in Africa. Folia Parasitologica , 57, 280– 288. Foitová Ivona, Baruš Vlastimil, Koubková Božena, Mašová Šárka , Nurcahyo Wisnu (2010): Description of Lemuricola ( Lemuricola ) pongoi —male (Nematoda: Enterobiinae) parasitising orangutan Pongo abelii . Parasitology Research , 106, 817–820. Moravec František, Jirk ů Miloslav, Charo-Karisa Harrison, Mašová Šárka (2009): Mexiconema africanum sp. n. (Nematoda: Daniconematidae) from the catfish Auchenoglanis occidentalis from Lake Turkana, Kenya. Parasitology Research , 105, 1047–1052. Mašová Šárka , Baruš Vlastimil, Hodová Iveta, Koubek Petr, Koubková Božena (2009): Redescription of Parapharyngodon micipsae (Seurat 1917) (Nematoda Pharyngodonidae) from the new host Tarentola parvicarinata Joger 1980 (Squamata Gekkonidae). Tropical Zoology , 22, 243–255. Mašová Šárka , Baruš Vlastimil, Hodová Iveta, Mat ějusová Iveta, Koubek Petr, Koubková Božena (2008): Morphometric and molecular characterization of Parapharyngodon echinatus (Nematoda, Pharyngodonidae) from the Senegal gecko ( Tarentola parvicarinata ). Acta Parasitologica , 53, 274–283.

11 International conference presentations

Mašová, Šárka, Baruš, Vlastimil Moravec, František Koubková, Božena, Hodová, Iveta Morphological studies on some African fish nematodes. In PARSA 2012. Oral presentation. Baruš, Vlastimil, Mašová, Šárka, Koubková, Božena, Sitko, Jiljí (2012): The remarkable nematode of the genus Subulura Molin, 1860 parasitizing in Otus scops (Strigiformes). In PARSA 2012. Poster. Mašová, Šárka, Kašparová, Eva (2012): Cystacanths of Corynosoma (Acanthocephala: Polymorphidae) from Notothenioid fish in the Prince Gustav Channel (Weddell sea). In UK Antarctic Science Conference 2012. Poster. Šmardová, Veronika, Mašová, Šárka, Baruš, Vlastimil, Hodová, Iveta, Sánchez, K. L., Foitová, Ivona (2012): A first record of the anoplocephalid tapeworm from a Javan Slow Loris (Nycticebus javanicus ) in Java, Indonesia. In XXIV Congress of the International Primatological Society. Poster. Mašová, Šárka, Baruš, Vlastimil, Seifertová, Mária (2011): Preliminary findings on African species complex Procamallanus (Procamallanus ) laeviconchus (Wedl, 1862). In VIII International Symposium of Fish Parasites. Oral presentation. Mašová, Šárka, Baruš, Vlastimil (2011): Procamallanus (Spirocamallanus ) spiralis (Camallanidae) in new host and locality - SEM and LM observations. VIII International Symposium of Fish Parasites. Poster. Mašová, Šárka, Baruš, Vlastimil (2010): How many species of the subgenus Procamallanus is really exist in African freshwater fishes? In ESN 2010 - Vienna (30th International Symposium of The European Society of Nematologists in Vienna). Oral presentation. Foitová, Ivona, Baruš, Vlastimil, Koubková, Božena, Mašová, Šárka (2010). Specialized pinworms of orang-utan ( Pongo abelii : Hominidae) from Sumatra. In ESN 2010 - Vienna (30th International Symposium of The European Society of Nematologists in Vienna). Poster. Mašová, Šárka, Baruš, Vlastimil, Moravec, František (2010): Procamallanus ( Procamallanus ) laeviconchus (Wedl , 1862) - Really the Only Species of the Subgenus Procamallanus Parasiting Fishes in Africa? In ICOPA 2010. (The XIIth International Congress of Parasitology, Melbourne, Australia). Poster. Blažek, Radim, Oros, Mikuláš, Mašová, Šárka, Dávidová, Martina, Jirk ů, Miloslav (2009): Fish parasites in the Lake Turkana Kenya. In XIII European Congress of Ichthyology (Klaipeda, Lithuania). Poster. Mašová, Šárka, Hodová, Iveta, Baruš, Vlastimil (2009): Studying of Nematode Morphology with Three Kinds of Methods: LM, CLSM and SEM. In Microscopy Conference 2009 in Graz (30 Aug – 4 Sept 2009). (Multinational Conference on Microscopy and the Dreiländertagung). Poster. Mašová, Šárka, Baruš, Vlastimil, Hodová, Iveta, Matějusová, Iveta, Koubek, Petr, Koubková, Božena (2007): Parapharyngodon echinatus (Nematoda: Pharyngodonidae) from the Senegal gecko ( Tarentola parvicarinata ): morphometrical and molecular characteristics. In Program and abstracts Tenth International Helminthological Symposium Helminths, Helminthoses and Environment. Poster.

National conference presentations

Michálková, Veronika, Mašová, Šárka, Jurajda, Pavel (2014): Diversity of fish parasites in Prince Gustav Channel (Weddell Sea), Antarctica – Expedition 2014: preliminary results. In 21 st Helminthological Days 2014. Poster.

12 Mašová, Šárka, P řikrylová, Iva (2013). Preliminary study on parasitofauna of Antarctic fishes in the proximity of James Ross Island (Prince Gustav Channel, Weddell Sea). Št ědronín u Orlíku - 20 th Helminthological Days. Poster. Mašová Šárka, Ivona Foitová, Vlastimil Baruš, Karmele Llano Sanchez, Wisnu Nurcahyo (2013): SEM of surface morphological structures of nematodes (Rictulariidae) parasitizing the Javan slow loris, Nycticebus javanicus in Indonesia. In Mikroskopie 2013. Poster. Mašová, Šárka, Baruš, Vlastimil, Kašparová, Eva (2012): Corynosoma acanthocephalans from paratenic hosts in the Weddel Sea, James Ross Island, Antarctica. In Polar Ecology Conference 2012. Poster. Mašová, Šárka, Šmardová, Veronika, Foitová, Ivona (2012): A coprological survey of pinniped parasites from James Ross Island (Antarctica). In X. české a slovenské parazitologické dny. Poster. Mašová, Šárka, Baruš, Vlastimil, Seifertová, Mária, Jirk ů, Miloslav (2012): How to distinguish crocodile ascarides. In X. české a slovenské parazitologické dny. Oral presentation. Šmardová, Veronika, Mašová, Šárka, Baruš, Vlastimil, Hodová, Iveta, Sánchez, K. L., Foitová, Ivona (2012): Tapeworms of Slow Loris with a new record of family Anoplocephalidae. In X. české a slovenské parazitologické dny. Poster. Baruš, Vlastimil, Mašová, Šárka, Koubková, Božena, Sitko, Jiljí (2012): The remarkable nematode of the genus Subulura Molin, 1860 parasitizing in Otus scops (Strigiformes) in the Czech Republic. In X. české a slovenské parazitologické dny. Poster. Kašparová, Eva, Mašová, Šárka, Ditrich, Oleg, Tyml, Tomáš, Tušer, Michal, Kodádková, Alena, Baruš, Vlastimil (2012): Anisakid nematode larvae from fishes of Petunia Bay (Svalbard). In X. české a slovenské parazitologické dny. Oral presentation. Mašová, Šárka, Kubí čková, Kristýna, Kašparová, Eva, Oleg, Tyml, Tomáš, Tušer, Michal, Kodádková, Alena, Baruš, Vlastimil (2012): Light and scanning electron microscopy of arctic anisakid larvae. In Mikroskopie 2012. Poster. Mašová, Šárka, Hodová, Iveta, Sonnek, Radim, Řehulková, Eva, Gelnar, Milan (2011): From light microscopy to microradiology: selected imaging methods in helminthology. In 19. Helmintologické dny. Invited oral presentation. Mašová, Šárka, Seifertová, Mária, Baruš, Vlastimil (2011): SEM and molecular characterization of Dujardinascaris madagascariensis (Heterocheilidae) - gastric crocodile nematode. In 19. Helmintologické dny. Poster. Petrášová, Jana, Peter Vallo, Petrželková, Klára, Foitová, Ivona, Koubková, Božena, Mašová, Šárka, Jirk ů, Milan, Scholz, Tomáš, Modrý, David (2011). Sdílíme tasemnice s našimi nejbližšími p říbuznými? Molekulární a morfologická studie anoplocephalidních tasemnic z primát ů. In XIII. konference mladých v ědeckých pracovník ů s mezinárodní ú častí, Veterinarni a farmaceuticka univerzita Brno. Oral presentation. Blažek, Radim, Dávidová, Martina, Jirsová, Dagmar, Mašová, Šárka, Oros, Mikuláš, P řikrylová, Iva, Řehulková, Eva, Jirk ů, Miloslav (2011). Fish assemblages and their parasites in the Lake Turkana, Kenya. In Zoologické Dny Brno. Poster. Mašová, Šárka, Wandrol, Petr, Baruš, Vlastimil (2011): Stereoscopic scanning electron micrographs of head structures of parasitic vertebrate nematode. In Mikroskopie 2011. Poster. Mašová, Šárka, Baruš, Vlastimil, Moravec, František, Koubková, Božena (2010): Hlístice čeledi Camallanidae z afrických sladkovodních ryb. In IX. Slovenské a české parazitologické dni. Oral presentation. Gelnar, Milan, Musilová, Na ďa, P řikrylová, Iva, Řehulková, Eva, Šafar číková, Lucie, Pátková, Monika, Šimková, Andrea, Dávidová, Martina, Mašová, Šárka, Koubková, Božena, Baruš, Vlastimil, Jirk ů, Miloslav, Ondra čková, Markéta, Blažek, Radim, Reichard, Martin (2010):

13 Cizopasníci sladkovodních ryb Afriky: sou časný stav a perspektivy. In IX. Slovenské a české parazitologické dni. Oral presentation. Mašová, Šárka, Baruš, Vlastimil, Moravec, František (2010): SEM observation on Procamallanus (Procamallanus) laeviconchus (Wedl, 1861). In 18. Helmintologické dny. Oral presentation. Mašová, Šárka, Baruš, Vlastimil (2010): Structure of cephalic end and eggs of Cithariniella khalili Petter, Vassiliades & Troncy, 1972 (Pharyngodonidae). In 18. Helmintologické dny. Poster. Gelnar, Milan, Musilová, Na ďa, Řehulková, Eva, P řikrylová, Iva, Mendlová, Monika, Dávidová, Martina, Mašová, Šárka, Koubková, Božena, Baruš, Vlastimil, Vetešníková Šimková, Andrea, Vysko čilová, Martina, Civá ňová, Kristina, Mat ějusová, Iveta, Blažek, Radim, Ondra čková, Markéta, Reichard, Martin, Vetešník, Lukáš (2010). Diverzita parazit ů sladkovodních ryb západní Afriky. In 18. Helmintologické dny. Oral presentation. Mašová, Šárka, Wandrol, Petr, Baruš, Vlastimil. (2010): Comparison of ESEM and SEM in morphology studies of parasitic vertebrate nematode. In Mirkoskopie 2010. Poster. Jirk ů, Miloslav, Blažek, Radim, Mašová, Šárka, Mikuláš, Oros. (2009): Ichtyoparasitology of the Lake Turkana, Kenya - project progress report. In 17th Helminthologial Days 2009. Oral presentation. Mašová, Šárka, Tenora, František, Baruš, Vlastimil, Koubek, Petr (2009): Interesting findings of two members of the genus Oochoristica (Cestoda, Anoplocephalidae, Linstowiinae) from reptiles (Senegal, Africa). In 17th Helminthological Days 2009. Poster. Mašová, Šárka, Baruš, Vlastimil, Moravec, František, Koubková, Božena (2009): Morphology of Brevimulticaecum heterotis (Ascarididae: Nematoda) studied by SEM and ESEM. In 17th Helminthological Days 2009. Oral presentation. Mašová, Šárka, Hodová, Iveta, Baruš, Vlastimil (2009): SEM and LM observations on Parapharyngodon micipsae (Nematoda, Pharyngodonidae) from Senegal. In Mikroskopia 2009 (25. 3. - 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia). Poster. Mašová, Šárka, Baruš, Vlastimil, Hodová, Iveta, Matějusová, Iveta, Koubek, Petr (2007): Morfometrická a molekulární charakteristika druhu Parapharyngodon echinatus (Nematoda: Pharyngodonidae) ze senegalského gekona ( Tarentola parvicarinata ). In Helmninthological Days 2006-2008. Book of Abstracts. Oral presentation.

Popularization seminars

2014 - Public lectures about Antarctica in Circus of Science on Masaryk University - Lecture for students about Antarctica - Secondary school of transport, trade and services, Moravský Krumlov 2013 - Lecture for students about Antarctica - Grammar School Víde ňská - Brno; Grammar School Hustope če - Public lecture about Antarctica in the cultural house Sázava; Rakvice Municipal library 2012 - Public lecture about Antarctica in Veronica (ecological institute) - Brno

14 Participation on grant projects

GBP505/12/G112 : ECIP - European Centre of Ichtyoparasitology (2012-2018; investigator: Assoc. Prof.. RNDr. Milan Gelnar, CSc.) BIDA : Biodiversity - the analyses of biological systems of different levels and on different scales of environment (2010-2012; investigator: Assoc. Prof. RNDr. Sv ětlana Zahrádková, Ph.D.) GD526/09/H025 : Evolutionary ecological analysis of communities and populations (2009-2012; investigator: Prof. Mgr. Stanislav Pekár, Ph.D.) Research Intents MSM0021622416 : Diversity of biotic communities and populations: causal analysis of variation in space and time (2005-2011; investigator: Assoc. Prof. RNDr. Milan Chytrý, Ph.D.) LC522: Ichtyoparazitology – the center of basic research (2005-2009; investigator: Assoc. Prof. RNDr. M. Gelnar, CSc.) IAA6093404 : Species diversity and ecology of selected West African vertebrates (2004-2008; investigator: Prof. RNDr. Petr Koubek, CSc.)