Pannexin 1 (PANX1) Rabbit Polyclonal Antibody Product Data

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Pannexin 1 (PANX1) Rabbit Polyclonal Antibody Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA319871 Pannexin 1 (PANX1) Rabbit Polyclonal Antibody Product data: Product Type: Primary Antibodies Applications: IF, WB Recommended Dilution: WB: 1 - 2 ug/mL, IF: 20 ug/mL Reactivity: Human, Mouse, Rat Host: Rabbit Isotype: IgG Clonality: Polyclonal Immunogen: Rabbit polyclonal PANX1 antibody was raised against a 17 amino acid peptide near the amino terminus of human PANX1. Formulation: PANX1 Antibody is supplied in PBS containing 0.02% sodium azide. Concentration: 1ug/ul Purification: PANX1 Antibody is affinity chromatography purified via peptide column. Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Predicted Protein Size: 47 kDa Gene Name: pannexin 1 Database Link: NP_056183 Entrez Gene 55991 MouseEntrez Gene 315435 RatEntrez Gene 24145 Human Q96RD7 Background: PANX1 Antibody: The pannexin gene family encodes a second class of putative gap junction proteins and are highly conserved in invertebrates and mammals. Pannexins (Panx) are four- pass transmembrane proteins that oligomerize to form large pore ion and metabolite- permeable channels. Pannexin-1 (PANX1) and Pannexin-3 are closely related, while Pannexin- 2 is a more distant relation. PANX1 is a transmembrane protein that forms a mechanosensitive ATP-permeable channel between adjacent cells and in the endoplasmic reticulum. PANX1 may play a role as a Ca2+ -leak channel to regulate ER Ca2+ homeostasis and regulates neural stem and progenitor cell proliferation. This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 Pannexin 1 (PANX1) Rabbit Polyclonal Antibody – TA319871 Synonyms: MRS1; PX1; UNQ2529 Protein Families: Transmembrane Product images: Western blot analysis of PANX1 in human ovary tissue lysate with PANX1 antibody at 1 ug/mL. Immunofluorescence of PANX1 in human ovary tissue with PANX1 antibody at 20 ug/mL. This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Biochemical and Functional Analyses of PANX1 Variants
Western University Scholarship@Western Electronic Thesis and Dissertation Repository June 2019 Biochemical and Functional Analyses of PANX1 Variants Daniel Nouri Nejad The University of Western Ontario Supervisor Penuela, Silvia The University of Western Ontario Graduate Program in Anatomy and Cell Biology A thesis submitted in partial fulfillment of the equirr ements for the degree in Master of Science © Daniel Nouri Nejad 2019 Follow this and additional works at: https://ir.lib.uwo.ca/etd Part of the Molecular Biology Commons Recommended Citation Nouri Nejad, Daniel, "Biochemical and Functional Analyses of PANX1 Variants" (2019). Electronic Thesis and Dissertation Repository. 6230. https://ir.lib.uwo.ca/etd/6230 This Dissertation/Thesis is brought to you for free and open access by Scholarship@Western. It has been accepted for inclusion in Electronic Thesis and Dissertation Repository by an authorized administrator of Scholarship@Western. For more information, please contact [email protected]. Abstract Pannexin 1 (PANX1) is a glycoprotein capable of forming large-pore single- membrane channels permeable to signaling molecules such as ATP. In this study, we interrogated different domains by introducing naturally occurring variants reported in melanoma and assessed their impact on the channel function of PANX1 at the cell surface. From this, we discovered a novel tyrosine phosphorylation site at Tyr150, that when disrupted via a missense mutation resulted in hypo-glycosylation and a greater capacity to traffic to the cell-surface and enhanced dye uptake. We have also uncovered a highly conserved ancestral allele, Gln5His, that has a greater allele frequency than the derived allele Gln5 in global and cancer cohorts but was not associated with cancer aggressiveness.
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Mouse Mol.Wt.: 48 Kda Panx1 Antibody
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A High-Throughput Approach to Uncover Novel Roles of APOBEC2, a Functional Orphan of the AID/APOBEC Family
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bioRxiv preprint doi: https://doi.org/10.1101/2021.06.03.446992; this version posted June 4, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. PWF et al. 2021 1 2 3 4 5 6 7 8 Panx1 channels promote both anti- and pro-seizure-like 9 activities in the zebrafish via p2rx7 receptors and ATP signaling 10 11 12 13 14 15 16 1,2,4* 1,2 1,2 1,2 17 Paige Whyte-Fagundes , Daria Taskina , Nickie Safarian , Christiane Zoidl , Peter L 18 Carlen3,4, Logan W Donaldson1, Georg R Zoidl1,2,4* 19 20 21 22 1 Department of Biology, York University; Toronto, Ontario, M3J1P3; Canada 23 2 Center of Vision Research (CVR), York University; Toronto, Ontario, M3J1P3; Canada 24 3 Department of Medicine, Physiology and BME, University of Toronto, 399 Bathurst St., 5w442, 25 Toronto, ON M5T 2S8, Canada 26 4 Department, Krembil Research Institute, University Health Network, 135 Nassau Street, 27 Toronto, ON M5T 1M8, Canada 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 *Corresponding authors: [email protected], [email protected] 45 46 Keywords: Pannexin1, seizures, epilepsy, electrophysiology, behavior, TALENs, Zebrafish, ATP 47 bioRxiv preprint doi: https://doi.org/10.1101/2021.06.03.446992; this version posted June 4, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
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