GENES

DEVELOPMENT

IN UPCOMING ISSUES ....

The lin-14 locus of Caenorhabditis elegans controls the time of expression of specific postembryonic development events

Victor Ambros and H. Robert Horvitz

Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA

Rosa Maria Ribas-Aparicio, Jason J. Sparkowski, Anne E. Proulx, John D. Mitchell, and Lawrence A. Klobutcher

Ultrabithorax mutations in common and variable regions of the protein coding sequence

Robert Weinzierl, J. Myles Axton, Alain Ghysen, and Michael Akarn

Retroviral transfer and expression of the IL-3 gene in hemopoietic cells

Peter M.C. Wong, Siu-Wah Chung, and Arthur W. Nienhuis

Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t 9 complementation group of the mouse t complex

Maja Bucan, Bernhard G. Herrmann, Anna-Maria Frischauf, Victoria L. Bautch, Vernon Bode, Lee M. Silver, Gail R. Martin, and Hans Lehrach

Activation and repression of mammalian gene expression by the c-myc protein

Rima Kaddurah-Daouk, John M. Green, Albert S. Baldwin, Jr., and Robert E. Kingston r I - I I IIII I ¢ ¢1 ",v " " b ~,O Q • ,¢ ,,~O COLD SPRING HARBOR LABORATORY CONFERENCE ON YEAST CELL BIOLOGY August 11-August 16 Organized by Amar Klar, Cold Spring Harbor Laboratory Paul Nurse, Imperial Cancer Research Fund (t~" (RESEAR_CHE_R S )Harvard Randy Schekman, University of California, Berkeley Co,n ZZ \ i S o. A specialized international Yeast meeting emphasiz- ing areas of cell cycle controls, developmental choices, cell- cell recognition, cytoskeleton and cell structure, protein targeting and modification, elements of chro- mosome structure and function, and other relevant areas will be held from August 11-16, 1987. Open call for abstracts. The abstract deadline is JUNE 2. For further information, contact: For ptpette ttps and other plastic Meetings Coordinator disposables, at prices that are Cold Spring Harbor Laboratory better-than-competitive: P.O. Box 100 NQRTECH LABORATORIES Cold Spring Harbor, NY 11724 4 NtdZand Avenue (516) 367.8346 HicksvtlZe, NY 11801 (516) 935-2040

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COLD SPRING HARBOR LABORATORY Conference on announces the sixth annual cancer cells conference on TRANSLATIONAL CONTROL EUKARYOTIC DNA REPLICATION September 16-20, 1987 September 2-6, 1987 Organized by: Organized by: Dr. John W.B. Hershey, University of California at Davis T.J. Kelly, Johns Hopkins University Dr. Michael B. Mathews, Cold Spring Harbor Laboratory B. Stillman, Cold Spring Harbor Laborator'y Dr. Brian Safer, National/nstitutes of Health Topics will include: Replication of Virus DNA Topics for discussion will include: Replication of Extra-Chromosomal Elements • mRNA structure and recognition Replication and Amplification of Chromosomal DNA • translation of specific mRNAs Control of Cell Cycle and S phase • stress phenomena (heat shock, etc.) Replication Proteins • viral systems Chromosome Structure and Segregation • differentiation, development and growth control A special session on Prokaryotic Model Systems • regulation of elongation and termination The meeting will include formal discussion sessions • genes for translational components and their regulation and poster sessions. The organizers invite submis- • protein secretion and processing s/on .of abstracts by June 24, 1987. Requests for Supported by a grant from/CN Biomedica/s, /nc. registration and/or abstract materials should be Registration and abstract materials are available from: forwarded to: • Meetings Coordinator Meetings Coordinator Cold Spring Harbor Laboratory COLD SPRING HARBOR LABORATORY P.O. Box 100 P.O. Box 100 Cold Spring Harbor, NY 11724 Cold Spring Harbor, N.Y. 11724 (516) 367-8346 J (516) 367-8345 ,iiiiiiii!!i~ii~

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.,.~--- =...: . Until now, when it came to preparative r 7 More importantly, the gel can be exposed DNA electrophoresis, researchers turn- as long as 20 minutes with little or no ed to 366nm Iongwave transilluminators detectable photonicking damage! to reduce photodamage, but sacrificed sensitivity. Today there's a solution. FOTO/PREP I comes with a replaceable UV transparent protective sheet, so gels can be cut directly FOTODYNE presents FOTO/PREP I. The first on its surface without damaging the UV glass. The molded, innovative transilluminator to offer the sensitiv- UV blocking cover can be raised from ity of 30Onto midrange UV, with the kind of pro- 0 ° to 90 ° to allow easy access to the gel tection previously provided only by 366nm UV sources. FOTO/PREP I. Available today, only from FOTODYNE. The leader you rely on for The key? A unique sensitivity control device. innovative advancements in DNA In the analytical mode it provides the nanogram analysis instrumentation. level sensitivity distinguishing all FOTODYNE 300nm DNA transilluminators. Switch to the preparative For more information call mode, and a 2000bp band containing less than 10 or write us. And ask for nanograms of DNA can be visualized for cutting. our free new catalog.

Call 1-800-DNA-FOTO III II FOTODYNE, INC. ~ 414-786-9550 See FOTO/PREP I at ASBC, 16700 W. Victor Road• or Booth 504-506, FOTODYNEINCORPORATED New Berlin, Wisconsin 53151-4131 U.S.A. TELEX260127 June 8-11, 1987. GENETIC MANIPULATION OF THE EARLY MAMMALIAN EMBRYO Banbury Report 20 Edited by Frank Costantini, Columbia University; RudolfJaenisch, Whitehead Institute and Department of Biology, Massachusetts Institute of Technology

Gene transfer techniques have brought a new power to bear upon fun- damental questions of mammaiian gene regulation and function during develop- ment. The fall 1984 Banbury conference on the genetic manipulation of the mammalian ovum and early embryo assembled major elements in such ap- proaches. Prominent among these approaches was the introduction of cloned genes directly into the mammalian germline, the introduc- tion of cells into early embryos, and the use of developmental muta- tions for identifying and isolating the specific genes affected. This volume contains the papers presented at that conference. As a col- lection of key strands of research in this area, this book should be particularly useful in gauging present capabilities as well as for gaining I a perspective on how this rapidly moving new field is likely to develop in the near future.

1985, 289 pp., illus., indexes LC 85-13233 CIP Cloth S63 ISBN 0-87969-220-0

MANIPULATING THE MOUSE EMBRYO

Originally developed as a laboratory manual for the Molecular Embryol- ogy of the Mouse course taught at Cold Spring Harbor Laboratory, MANIPULATING THE MOUSE EMBRYO has been expanded to provide a A Laboratory Manual detailed and up-to-date compendium of the techniques involved in the study of early mouse development and in the genetic manipulation of the mouse em- By bryo. Beginning with a history of the mouse as an experimental animal for the study of the genetics of mammalian development and a review of current B.L.M. Hogan, National Institute for Medical Research knowledge on early mouse development at the cellular and molecular levels, F. Costantini, Columbia University the book then moves on to describe isolation of pre- and postimplantation em- bryos, embryo culture, embryo transfer and other experimental procedures on E. Lacy, Memorial SIoan-Kettering Cancer Center mice, microinjection of DNA into fertilized mouse eggs, nuclear transplanta- tion, isolation and culture of embryonic cell lines, and chimera formation by injection of stem cells into the blastocyst. Because it assumes no prior ex- perience in the handling of the mouse embryo, this amply illustrated manual will be useful to scientists who wish to work with these materials for the first time and to students in graduate-level courses in developmental biology, as well as to experienced embryologists interested in exploring and applying new techniques such as gene transfer by DNA microinjection.

, , 1986, 332 pp., LC 84-17628 CIP illus., colorplates, appendix, bibliography, index ISBN 0-87969-175-1 Paper $60 Q Cold Spring Harbor Laboratory P.O. Box 100 Cold SpringHarbor, New York 11724 Cold Spring Harbor Symposia Molecular Biology

Thirteen years marked the time between the discovery of the double helix in 1953 and the elucidation of the genetic code in 1966. A similar interval has now passed since the development by Cohen and Boyer of a simple procedure for the cloning of selective DNA fragments. The scientific advances made possible by the subsequent modification and elaboration of these original cloning procedures are increasingly overwhelming. Facts that until recently were virtually unobtainable now flow forth almost effortlessly. Most excitingly, the frenetic pace of these new discoveries, instead of marking the impending end of a glorious moment of learning, give every indication of opening up scientific frontiers that will take many years to explore thoroughly. This new era of enlightenment is nowhere more apparent than in the newfound ability to study man at the molecular level. By focusing on the molecular biology of Homo sapiens as the topic for its 51st Symposium, Cold Spring Harbor Laboratory chose a topic that most certainly will be returned to over and over during the second 50 years ofits Symposium.

CONTENTS Applications: Cystic Fibrosis, Muscular Dystrophy, Huntington's Disease, Hemophilia A, Down's Syndrome, PKU, and Heart Disease INTRODUCTION (W.F. Bodmer) Cystic fibrosis: The basic defect (R. Williamson et al.); RFLP probes HUMAN GENE MAP as diagnostic tools (H. Donis-Keller et al.); Cystic fibrosis map- ping (L.-C. Tsui et al.); X-linked disease (K.E. Davies et al.); Summaries Additions and Recent Translocations and muscular dystrophy (R.G. Worton et al.); The human gene map (V.A. McKusick); Linkage approaches to Genetics of DMD (L.M. Kunkel et al.); Gene analysis of DMD gene localization (R. White et al.); Analytical strategies for (P.L. Pearson et al.); Huntington's disease (.J.F. Gusella et al.); genetic mapping (J.-M. Lalouel et al.); Mapping complex genetic Cloned factor VIII (R.M. Lawn et al.); Deficiency alleles of traits (E. Lander, D. Botstein); Human MHC (J.L. Strominger); 3-globin and factor VIII:C genes (H.H. Kazazian, Jr. et al.); HLA class-II genes (B. Mach et al.); Molecular biology of the Overexpression of transfected h-CuZnSOD gene and DS (Y. MHC (J.I. Bell et al.); Class II RFLPs (S.W. Serjeantson et al.); Groner et al.); Carrier screening for PKU and somatic gene Human DNA repak gene ERCC-1 (J.H.J. Hoeijmakers et al.); therapy (S.L.C. Woo et al.); Molecular biology of coronary Human mtDNA-encoded NADH dehydrogenase subunits (G. arteriosclerosis (S. Deeb et al.) Attardi et al.) HUMAN EVOLUTION New Mapping Strategies Human genetic variation (L.L. Cavalli-Sforza et al.); Fossil evidence (P. Andrews); Evolution of Ig genes in primates (S. Physical mapping methods (C.L. Smith, C.R. Cantor); Mapping Ueda et al.); Rate of human mtDNA evolution (M. Stoneldng et and cloning megabase DNA (S. K. Lawrance et al.); Molecular ap- al.); Paleomolecular biology of human remains (S. P/iiibo); proaches to mammalian genetics (A. Poustka et al.); Flow cyto- Human protein sequences (R.F. Doolittle et al.); LINE-1 family genetics (J.W. Gray et al.); Fluorescence hybridization (D. Pinkel of genes and pseudogenes (J. Skowronski, M.F. Singer); L-1 and et al.); Chromosome-specific DNA libraries (L.L. Deaven et al.); reverse transcriptase (Y. Sakaki et al.); I. A/u evolution II. Flow-sorting analysis of the human genome (R.V. Lebo et al.); Human transposon (I. Sawada et al.); The human genome (G. Cloning the X-CGD gene by linkage (B. Royer-Pokora et al); Bemardi, G. Bernardi); c,-Thalassemia and the malaria Nondisjunction in Trisomy 21 (S.E. Antonarakis et al.) hypothesis (A.V.S. Hill); Primate (J. Marks et al.) DRUGS MADE OFF HUMAN GENES Recombination along Sex Chromosomes Clotting, Anti-dotting Factors Genetic recombination and disease (M. Siniscalco); Genetic map- Molecular assembly of plasma proteins (E.W. Davie et al.); yon ping of the X chromosome (J.L. Mandel et al.); Molecular Willebrand factor (J.E. Sadler et al.); Evolution of vitamin-K- genetics of MIC2 (S.M. Darling et al.); Human telomeres (H.J. dependent coagulation factors (G.L Long); Human factor VII Cooke, B.A. Smith); Human X/Y pseudoautosomal region (F. cDNA isolation and expression (K. Berkner et al.); Structure- Rouyer et al.); Sex reversal and the Y chromosome (D.C. Page); function relationships in human factor VIII (J.J. Toole et al.); Molecular biology and pathology of the human Y chromosome Recombinant t-PA (G.A. Vehar et al.); Properties of scu-PA (E. Seboun et al.); 46,XX and 45,X males (A. de la Chapelle) (D.C. Stump et al.) Anti-cancer Agents GENETIC DIAGNOSIS Lymphoblastoid interferon production (N.B. Finter et al.); The Development of New Methodologies interleukin-2 system (T. Taniguchi et al.); Lymphokines and Analysis of DNA sequence variants in man (R.B. Wallace et al.); monokines in anticancer therapy (W. Fiefs et al.); Tumor necrosis Polymerase chain reaction (K. Mullis et al.); Detection of single, factors (D.V. Goeddel et al.); Tandem of TNF / cachectin and lym- base mutations in human DNA (R.M. Myers, T. Maniatis); Search- photoxin genes (S.A. Nedospasov et al.); Cachectin: Dark side of ing for gene defects (L.S. Lerman et al.); Molecular probes of TNF (A. Cerami, B. Beutler); Interleukin-1 (P.T. Lomedico et all); chromosome aberrations (S.A. Lattet al.) Mullerian-inhibiting substance (R.L. Care et al.)

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- .., ~ o = ~ ~ ~*"~ ~ ~ancer ~;ellS (S.L. Watts et al.); Cellular origin of condylomata acuminata (J. Buscema et al.); HPV transformation (P. Kaur, J.K. PAPILLOMAVIRUSES McDougall); Transformation induced by HPV-16 DNA (J.A. DiPaolo et al.); Interaction of papillomaviruses and car- Edited by cinogens (E. Amtmann et al.); BPV transformation and Bettie Steinberg and Janet Brandsma, Long cellular responses (K.T. Smith et al.) Island Jewish Medical Center Relatlonshlp of Papillomaviruses Lorne Taichman, State University of New York, to Human and Animal Diseases Stony Brook HPV and cervical cancer (L. Gissmann et al.); Biological Studies on the papillomaviruses have expanded potential of cervical HPV infections (K. Syrj~nen et al.); Analysis of malignant tissues for HPV DNA (R.S. Ostrow et markedly over the past five years due to the al.); Papillomavirus DNA in cervical tumors (P.G. Fuchs et availability of recombinant reagents and in vitro al.); Papillomavirus infection of the nose (J. Brandsma et systems and to the recognition of the strong al.); Human cutaneous papillomas (S. Jablonska et al.); association of specific papillomaviruses with cer- HPV type-specific markers in cervical lesions (P. Mose tain carcinomas in humans. This book addresses Larsen et al.); Comparison of in situ DNA hybridizations (S. molecular aspects of the virus in both transforma- Syrj~nen et al.); Transcription of HPV-16 in genital pre- tion and replication and also discusses several cancers (G. Nuovo et al.); HPV and c-myc in cervical can- features of the molecular epidemiology of the cer (P. Gariglio et al.); HPV integration in dysplasia (K. human papillomaviruses. Shimoda, W.D. Lancaster); Homozygous integration of pap- illomavirus (P.A. Lazo); Viral tumors in inbred rabbits (A. CONTENTS Seto et al.); Mouse papillomavirus (J.P. Sundberg et al.) Introduction (P.M. Howley) Immunology and Therapy Transcription NCMC in HPV-induced anogenital lesions (J. Malejczyk et al.); Anti-BPV immunity in patients with HPV infections BPV-1 E2 trans-activation (B.A. Spalholz et al.); BPV-1 en- (B.K. Beiss et al.); Therapies for HPV diseases (P.K. codes a transcriptional repressor activity (P.F. Lambert et Weck, J.K. Whisnant); Interferon response and HPV (B.M. al.); HPV-18 transcription (F. Thierry et al.); HPV-8 en- Steinberg et al.); Hematoporphyrin photodynamic ther- hancer and BPV-1 E2 (R. Seeberger et al.); trans-Activation apy and CRPV (M.J. Shikowitz et al.) of a BPV early gene promoter (T.H. Haugen et al.); HPV-18 in cell lines and human cell hybrids (E. Schwarz et al.); HPV June 1987, 480 pp. (approx.), gene expression (L.T. Chow et al.); RNA probes of HPV lllus., Indexes LC 87-8030 transcription in respiratory papillomata (P. Ward et al.) Paper $80 ISBN 0-87969-301-0 Paplllomavlrus-assoclated Protelns DNA-binding activity of BPV E2 (E.J. Androphy et al.); E5 polypeptide (R. Schlegel, M. Wade-Glass); Characteriza- ALSO AVAILABLE tion of papillomavirus proteins (R.G. MaJIon et al.); HPV • Cancer Cells 4/DNA TUMOR VIRUSES: synthetic peptides (J.M. Palefsky et al.); HPV-6b and Control of Gene Expresslon and Repllcatlon HPV-16 antibodies (J.M. Firzlaff et al.); HPV-1 E4 proteins Edited by Michael Botchan, University of California; Terri in warts (F. Breitburd et al.); Expression of HPV, la late Grodzicker, Cold Spring Harbor Laboratory; Philip Sharp, ORFs (J. Campione-Piccardo et al.); Provoked late gene Massachusetts Institute of Technology expression (N.A. Jensen et al.); Papillomavirus-specific 1986, 620 pp., lllus., Indexes LC 86-50649 CIP protein inductions (R.M. Levenson et al.); E6-E7 structure Paper $75 ISBN 0-87969-192-1 (O. Danos, M. Yaniv); Transforming-agent-specific secret- • Cancer Cells 3 ed proteins (U.G. Brinckmann et al.) GROWTH FACTORS AND TRANSFORMATION Repllcatlon Edited by James Feramisco, Cold Spring Harbor Labora- Underreplication of HPV-1 DNA (S.S. Reilly, L.B. Taich- tory; Brad Ozanne, University of Texas Health Science man); HPV-1 infection of cultured respiratory cells (C.B. Center; Charles Stiles, Dana-Farber Cancer Institute. Christian et al.); Transfection of keratinocytes (A. Farr et 1985, 450 pp., Illus., Indexes LC 85-3733 CIP al.); HPV amplification (C.R. Brandt et al.) Paper $70 ISBN 047969.178-6 Genetics of Transformation • Cancer Cells 2/ONCOGENES AND VIRAL GENES Mutational analysis of BPV-1 (D. DiMaio et al.); Transfor- Out of print mation with HPV type-16 DNA (G. Matlashewski et al.); • Cancer Cells 1/THE TRANSFORMED PHENOTYPE Transformation by HPV-16 and HPV-18 (L.A. Laimins et Edited by A.J. Levine, State University of New York at al.); Transforming gene of BPV-1 (P. Bergman et al.) Stony Brook; G.F. Vande Woude, National Cancer Insti- tute, National Institutes of Health; W.C. Topp, Cold Spring Transformation In Vltro and In Vlvo Harbor Laboratory," J.D. Watson, Cold Spring Harbor HPV-11 expression (J.W. Kreider et al.); BPV-l-transformed Laboratory rodent fibroblasts (B. Bin~truy et al.); Progression of CRPV 1984, 385 pp., Bus., index LC 83-26318 CIP +l i,-.~r~ l~ (~,-,h~;t-4~r_kA~* i~r~i .r~. ~÷ ,'~1 ~" LID~I ;~ ,-,~11 ,-,~ ,it, ,r~ B~A. ~E~ l~S&l A aqALA 4L~ d~