GENES DEVELOPMENT IN UPCOMING ISSUES .... The lin-14 locus of Caenorhabditis elegans controls the time of expression of specific postembryonic development events Victor Ambros and H. Robert Horvitz Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA Rosa Maria Ribas-Aparicio, Jason J. Sparkowski, Anne E. Proulx, John D. Mitchell, and Lawrence A. Klobutcher Ultrabithorax mutations in common and variable regions of the protein coding sequence Robert Weinzierl, J. Myles Axton, Alain Ghysen, and Michael Akarn Retroviral transfer and expression of the IL-3 gene in hemopoietic cells Peter M.C. Wong, Siu-Wah Chung, and Arthur W. Nienhuis Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t 9 complementation group of the mouse t complex Maja Bucan, Bernhard G. Herrmann, Anna-Maria Frischauf, Victoria L. Bautch, Vernon Bode, Lee M. Silver, Gail R. Martin, and Hans Lehrach Activation and repression of mammalian gene expression by the c-myc protein Rima Kaddurah-Daouk, John M. Green, Albert S. Baldwin, Jr., and Robert E. Kingston r I - I I IIII I ¢ ¢1 ",v " " b ~,O Q • ,¢ ,,~O COLD SPRING HARBOR LABORATORY CONFERENCE ON YEAST CELL BIOLOGY August 11-August 16 Organized by Amar Klar, Cold Spring Harbor Laboratory Paul Nurse, Imperial Cancer Research Fund (t~" (RESEAR_CHE_R S )Harvard Randy Schekman, University of California, Berkeley Co,n ZZ \ i S o. A specialized international Yeast meeting emphasiz- ing areas of cell cycle controls, developmental choices, cell- cell recognition, cytoskeleton and cell structure, protein targeting and modification, elements of chro- mosome structure and function, and other relevant areas will be held from August 11-16, 1987. Open call for abstracts. The abstract deadline is JUNE 2. For further information, contact: For ptpette ttps and other plastic Meetings Coordinator disposables, at prices that are Cold Spring Harbor Laboratory better-than-competitive: P.O. Box 100 NQRTECH LABORATORIES Cold Spring Harbor, NY 11724 4 NtdZand Avenue (516) 367.8346 HicksvtlZe, NY 11801 (516) 935-2040 r r Q Q COLD SPRING HARBOR LABORATORY Conference on announces the sixth annual cancer cells conference on TRANSLATIONAL CONTROL EUKARYOTIC DNA REPLICATION September 16-20, 1987 September 2-6, 1987 Organized by: Organized by: Dr. John W.B. Hershey, University of California at Davis T.J. Kelly, Johns Hopkins University Dr. Michael B. Mathews, Cold Spring Harbor Laboratory B. Stillman, Cold Spring Harbor Laborator'y Dr. Brian Safer, National/nstitutes of Health Topics will include: Replication of Virus DNA Topics for discussion will include: Replication of Extra-Chromosomal Elements • mRNA structure and recognition Replication and Amplification of Chromosomal DNA • translation of specific mRNAs Control of Cell Cycle and S phase • stress phenomena (heat shock, etc.) Replication Proteins • viral systems Chromosome Structure and Segregation • differentiation, development and growth control A special session on Prokaryotic Model Systems • regulation of elongation and termination The meeting will include formal discussion sessions • genes for translational components and their regulation and poster sessions. The organizers invite submis- • protein secretion and processing s/on .of abstracts by June 24, 1987. Requests for Supported by a grant from/CN Biomedica/s, /nc. registration and/or abstract materials should be Registration and abstract materials are available from: forwarded to: • Meetings Coordinator Meetings Coordinator Cold Spring Harbor Laboratory COLD SPRING HARBOR LABORATORY P.O. Box 100 P.O. Box 100 Cold Spring Harbor, NY 11724 Cold Spring Harbor, N.Y. 11724 (516) 367-8346 J (516) 367-8345 ,iiiiiiii!!i~ii~ iiiiiiiiii::, .,.~--- =...: . Until now, when it came to preparative r 7 More importantly, the gel can be exposed DNA electrophoresis, researchers turn- as long as 20 minutes with little or no ed to 366nm Iongwave transilluminators detectable photonicking damage! to reduce photodamage, but sacrificed sensitivity. Today there's a solution. FOTO/PREP I comes with a replaceable UV transparent protective sheet, so gels can be cut directly FOTODYNE presents FOTO/PREP I. The first on its surface without damaging the UV glass. The molded, innovative transilluminator to offer the sensitiv- UV blocking cover can be raised from ity of 30Onto midrange UV, with the kind of pro- 0 ° to 90 ° to allow easy access to the gel tection previously provided only by 366nm UV sources. FOTO/PREP I. Available today, only from FOTODYNE. The leader you rely on for The key? A unique sensitivity control device. innovative advancements in DNA In the analytical mode it provides the nanogram analysis instrumentation. level sensitivity distinguishing all FOTODYNE 300nm DNA transilluminators. Switch to the preparative For more information call mode, and a 2000bp band containing less than 10 or write us. And ask for nanograms of DNA can be visualized for cutting. our free new catalog. Call 1-800-DNA-FOTO III II FOTODYNE, INC. ~ 414-786-9550 See FOTO/PREP I at ASBC, 16700 W. Victor Road• or Booth 504-506, FOTODYNEINCORPORATED New Berlin, Wisconsin 53151-4131 U.S.A. TELEX260127 June 8-11, 1987. GENETIC MANIPULATION OF THE EARLY MAMMALIAN EMBRYO Banbury Report 20 Edited by Frank Costantini, Columbia University; RudolfJaenisch, Whitehead Institute and Department of Biology, Massachusetts Institute of Technology Gene transfer techniques have brought a new power to bear upon fun- damental questions of mammaiian gene regulation and function during develop- ment. The fall 1984 Banbury conference on the genetic manipulation of the mammalian ovum and early embryo assembled major elements in such ap- proaches. Prominent among these approaches was the introduction of cloned genes directly into the mammalian germline, the introduc- tion of cells into early embryos, and the use of developmental muta- tions for identifying and isolating the specific genes affected. This volume contains the papers presented at that conference. As a col- lection of key strands of research in this area, this book should be particularly useful in gauging present capabilities as well as for gaining I a perspective on how this rapidly moving new field is likely to develop in the near future. 1985, 289 pp., illus., indexes LC 85-13233 CIP Cloth S63 ISBN 0-87969-220-0 MANIPULATING THE MOUSE EMBRYO Originally developed as a laboratory manual for the Molecular Embryol- ogy of the Mouse course taught at Cold Spring Harbor Laboratory, MANIPULATING THE MOUSE EMBRYO has been expanded to provide a A Laboratory Manual detailed and up-to-date compendium of the techniques involved in the study of early mouse development and in the genetic manipulation of the mouse em- By bryo. Beginning with a history of the mouse as an experimental animal for the study of the genetics of mammalian development and a review of current B.L.M. Hogan, National Institute for Medical Research knowledge on early mouse development at the cellular and molecular levels, F. Costantini, Columbia University the book then moves on to describe isolation of pre- and postimplantation em- bryos, embryo culture, embryo transfer and other experimental procedures on E. Lacy, Memorial SIoan-Kettering Cancer Center mice, microinjection of DNA into fertilized mouse eggs, nuclear transplanta- tion, isolation and culture of embryonic cell lines, and chimera formation by injection of stem cells into the blastocyst. Because it assumes no prior ex- perience in the handling of the mouse embryo, this amply illustrated manual will be useful to scientists who wish to work with these materials for the first time and to students in graduate-level courses in developmental biology, as well as to experienced embryologists interested in exploring and applying new techniques such as gene transfer by DNA microinjection. , , 1986, 332 pp., LC 84-17628 CIP illus., colorplates, appendix, bibliography, index ISBN 0-87969-175-1 Paper $60 Q Cold Spring Harbor Laboratory P.O. Box 100 Cold SpringHarbor, New York 11724 Cold Spring Harbor Symposia Molecular Biology Thirteen years marked the time between the discovery of the double helix in 1953 and the elucidation of the genetic code in 1966. A similar interval has now passed since the development by Cohen and Boyer of a simple procedure for the cloning of selective DNA fragments. The scientific advances made possible by the subsequent modification and elaboration of these original cloning procedures are increasingly overwhelming. Facts that until recently were virtually unobtainable now flow forth almost effortlessly. Most excitingly, the frenetic pace of these new discoveries, instead of marking the impending end of a glorious moment of learning, give every indication of opening up scientific frontiers that will take many years to explore thoroughly. This new era of enlightenment is nowhere more apparent than in the newfound ability to study man at the molecular level. By focusing on the molecular biology of Homo sapiens as the topic for its 51st Symposium, Cold Spring Harbor Laboratory chose a topic that most certainly will be returned to over and over during the second 50 years ofits Symposium. CONTENTS Applications: Cystic Fibrosis, Muscular Dystrophy, Huntington's Disease, Hemophilia A, Down's Syndrome, PKU, and Heart Disease INTRODUCTION (W.F. Bodmer) Cystic fibrosis: The basic defect (R. Williamson et al.); RFLP probes HUMAN GENE MAP as diagnostic tools (H. Donis-Keller et al.); Cystic fibrosis map- ping (L.-C. Tsui et al.); X-linked disease (K.E. Davies et al.); Summaries Additions and Recent Translocations and muscular dystrophy (R.G. Worton et al.); The human gene map (V.A. McKusick); Linkage approaches to Genetics of DMD (L.M. Kunkel et al.); Gene analysis of DMD gene localization (R. White et al.); Analytical strategies for (P.L. Pearson et al.); Huntington's disease (.J.F. Gusella et al.); genetic mapping (J.-M. Lalouel et al.); Mapping complex genetic Cloned factor VIII (R.M. Lawn et al.); Deficiency alleles of traits (E. Lander, D.