Kiran Light Laboratories 2012-13 Final
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Crystallization and Preliminary X-Ray Analysis of Ocr, the Product of Gene 0.3 of Bacteriophage T7
Edinburgh Research Explorer Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7 Citation for published version: Sturrock, SS, Dryden, DTF, Atanasiu, C, Dornan, J, Bruce, S, Cronshaw, A, Taylor, P & Walkinshaw, MD 2001, 'Crystallization and preliminary X-ray analysis of ocr, the product of gene 0.3 of bacteriophage T7', Acta Crystallographica Section D: Biological Crystallography, vol. 57, no. Part 11, pp. 1652-1654. https://doi.org/10.1107/S0907444901011623 Digital Object Identifier (DOI): 10.1107/S0907444901011623 Link: Link to publication record in Edinburgh Research Explorer Document Version: Publisher's PDF, also known as Version of record Published In: Acta Crystallographica Section D: Biological Crystallography Publisher Rights Statement: RoMEO green General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 27. Sep. 2021 crystallization papers Acta Crystallographica Section D Crystallization and preliminary X-ray analysis of Biological Crystallography ocr, the product of gene 0.3 of bacteriophage T7 ISSN 0907-4449 Shane S. Sturrock,a David T. F. -
S-Carboxymethylcysteine Synthase from Escherichia Coli
Agric. Biol. Chem., 53 (9), 2481 -2487, 1989 2481 S-Carboxymethylcysteine Synthase from Escherichia coli Hidehiko Kumagai,* Hideyuki Suzuki, Hiroki Shigematsu and Tatsurokuro Tochikura Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan Received April 14, 1989 An enzyme that catalyzes the synthesis of S-carboxymethyl-L-cysteine from 3-chloro-L-alanine (3-Cl-Ala) and thioglycolic acid was found in Escherichia coli W3110 and was designated as S- carboxymethyl-L-cysteine synthase. It was purified from the cell-free extract to electrophoretic homogeneity and was crystallized. The enzymehas a molecular weight of 84,000 and gave one band corresponding to a molecular weight of 37,000 on SDS-polyacrylamide gel electrophoresis. The purified enzyme catalyzed the ^-replacement reactions between 3-Cl-Ala and various thiol compounds. The apparent Kmvalues for 3-Cl-Ala and thioglycolic acid were 40mMand 15.4mM.The enzyme showedvery low activity as to the a,/^-elimination reaction with 3-Cl-Ala and L-serine. It was not inactivated on the incubation with 3-Cl-Ala. Theabsorption spectrum of the enzymeshows a maximum at 412nm, indicating that it contains pyridoxal phosphate as a co factor. The N-terminal amino acid sequence was determined and the corresponding sequence was detected in the protein sequence data bank, but no homogeneous sequence was found. 3-Chloro-L-alanine (3-Cl-Ala) acts as a so- called suicide substrate for some pyridoxal- Materials and Methods phosphate (PLP) dependent enzymes, such Reagents. Casamino acids and Tryptone were obtained as glutamate-oxaloacetic acid transminase,1} from Difco Laboratories, yeast extract from Oriental glutamate-pyruvic acid transaminase2) and Yeast Co., amido black 10B from E. -
Biological Stains & Dyes
BIOLOGICAL STAINS & DYES Developed for Biology, microbiology & industrial applications ACRIFLAVIN ALCIAN BLUE 8GX ACRIDINE ORANGE ALIZARINE CYANINE GREEN ANILINE BLUE (SPIRIT SOLUBLE) www.lobachemie.com BIOLOGICAL STAINS & DYES Staining is an important technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues. Loba Chemie offers comprehensive range of Stains and dyes, which are frequently used in Microbiology, Hematology, Histology, Cytology, Protein and DNA Staining after Electrophoresis and Fluorescence Microscopy etc. Many of our stains and dyes have specifications complying certified grade of Biological Stain Commission, and suitable for biological research. Stringent testing on all batches is performed to ensure consistency and satisfy necessary specification particularly in challenging work such as histology and molecular biology. Stains and dyes offer by Loba chemie includes Dry – powder form Stains and dyes as well as wet - ready to use solutions. Features: • Ideally suited to molecular biology or microbiology applications • Available in a wide range of innovative chemical packaging options. Range of Biological Stains & Dyes Product Code Product Name C.I. No CAS No 00590 ACRIDINE ORANGE 46005 10127-02-3 00600 ACRIFLAVIN 46000 8063-24-9 00830 ALCIAN BLUE 8GX 74240 33864-99-2 00840 ALIZARINE AR 58000 72-48-0 00852 ALIZARINE CYANINE GREEN 61570 4403-90-1 00980 AMARANTH 16185 915-67-3 01010 AMIDO BLACK 10B 20470 -
Electrophoresis Proteins Enzymes
ÚSTAV LÉKAŘSKÉ BIOCH EMIE A LABORATORNÍ D IAGNOSTIKY 1. LF UK A VFN Electrophoresis Proteins Enzymes Practical lesson on medical biochemistry General Medicine Jan Pláteník 2020/2021 1 1 Electrophoresis of serum proteins in 0.5% agarose Native electrophoresis of serum proteins in agarose gel is still one of the basic examinations in clinical chemistry, and in our practical lesson serves as a general example of electrophoretic separation of proteins. In this arrangement of electrophoresis the proteins are native, i.e., not denatured. In an alkaline buffer (pH 8.5-9) they gain negative charges and migrate from the negative electrode to the positive one. The support consists of an agarose gel, which, unlike acrylamide, has pores too large to substantially limit the protein movement. As a result, the proteins are separated according to their surface charge densities. If resolved in this way, the human serum yields several classical protein fractions: albumin moves the farthest, followed by several globulin bands, denoted consecutively as α1, α2, β (usually separated to β1 and β2), and finally γ globulins. + Albumin α1-globulins α2-globulins β1-globulins β2-globulins γ-globulins − Typical results of an authentic electrophoretic separation of human serum proteins, including densitometric evaluation (assembled from pictures available at www.sebia.com). The whole experiment consists of several steps: • Casting the agarose gel: agarose is a polysaccharide galactan obtained from seaweed. In order to get a liquid agarose solution, the mixture of buffer and solid agarose must be heated to boiling, then during cooling the agarose fibers in the solution non-covalently associate and form a gel. -
Chemical Names and CAS Numbers Final
Chemical Abstract Chemical Formula Chemical Name Service (CAS) Number C3H8O 1‐propanol C4H7BrO2 2‐bromobutyric acid 80‐58‐0 GeH3COOH 2‐germaacetic acid C4H10 2‐methylpropane 75‐28‐5 C3H8O 2‐propanol 67‐63‐0 C6H10O3 4‐acetylbutyric acid 448671 C4H7BrO2 4‐bromobutyric acid 2623‐87‐2 CH3CHO acetaldehyde CH3CONH2 acetamide C8H9NO2 acetaminophen 103‐90‐2 − C2H3O2 acetate ion − CH3COO acetate ion C2H4O2 acetic acid 64‐19‐7 CH3COOH acetic acid (CH3)2CO acetone CH3COCl acetyl chloride C2H2 acetylene 74‐86‐2 HCCH acetylene C9H8O4 acetylsalicylic acid 50‐78‐2 H2C(CH)CN acrylonitrile C3H7NO2 Ala C3H7NO2 alanine 56‐41‐7 NaAlSi3O3 albite AlSb aluminium antimonide 25152‐52‐7 AlAs aluminium arsenide 22831‐42‐1 AlBO2 aluminium borate 61279‐70‐7 AlBO aluminium boron oxide 12041‐48‐4 AlBr3 aluminium bromide 7727‐15‐3 AlBr3•6H2O aluminium bromide hexahydrate 2149397 AlCl4Cs aluminium caesium tetrachloride 17992‐03‐9 AlCl3 aluminium chloride (anhydrous) 7446‐70‐0 AlCl3•6H2O aluminium chloride hexahydrate 7784‐13‐6 AlClO aluminium chloride oxide 13596‐11‐7 AlB2 aluminium diboride 12041‐50‐8 AlF2 aluminium difluoride 13569‐23‐8 AlF2O aluminium difluoride oxide 38344‐66‐0 AlB12 aluminium dodecaboride 12041‐54‐2 Al2F6 aluminium fluoride 17949‐86‐9 AlF3 aluminium fluoride 7784‐18‐1 Al(CHO2)3 aluminium formate 7360‐53‐4 1 of 75 Chemical Abstract Chemical Formula Chemical Name Service (CAS) Number Al(OH)3 aluminium hydroxide 21645‐51‐2 Al2I6 aluminium iodide 18898‐35‐6 AlI3 aluminium iodide 7784‐23‐8 AlBr aluminium monobromide 22359‐97‐3 AlCl aluminium monochloride -
The Synthesis, Characterisation and Ion Exchange of Mixed Metal Phosphates by Victoria Anne Burnell
The Synthesis, Characterisation and Ion Exchange of Mixed Metal Phosphates by Victoria Anne Burnell A thesis submitted to The University of Birmingham for the degree of Doctor of Philosophy The School of Chemistry College of Engineering and Physical Sciences University of Birmingham August 2011 University of Birmingham Research Archive e-theses repository This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. Abstract Abstract This thesis presents work investigating the synthesis and characterisation of a range of mixed metal phosphates. The main aim of the project was to develop materials for the remediation of nuclear waste. In light of this, the materials developed were subject to ion exchange studies and leach testing. The thermal behaviour of the phosphates and nature of the decomposition products were also investigated. This study demonstrates that the true solid solution cannot be formed in any of the mixed metal series investigated, which included zirconium-titanium, germanium-titanium, germanium-zirconium, tin-titanium and tin-zirconium. In all cases a miscibility gap was observed and the reasons for these were established. The co-precipitates that formed were characterised by a variety of analytical techniques which included powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), pair distribution function (PDF) analysis and X-ray fluorescence (XRF). -
Intermediates-3 1/89 Page
Intermediates-3 1/89 page CAS Chemical Name 4163-60-4 BETA-D-GALACTOSE PENTAACETATE 129722-12-9 Aripiprazole 80-08-0 Bis(4-aminophenyl) Sulfone 92-88-6 4,4'-biphenol 1344-28-1 Aluminum oxide 18680-27-8 (1S,2S,3R,5S)-(+)-2,3-PINANEDIOL 4045-44-7 1,2,3,4,5-PENTAMETHYLCYCLOPENTADIENE 78-96-6 DL-1-Amino-2-propanol 2687-43-6 O-benzylhydroxy lamine hydrochloride 141-33-3 SODIUM BUTYL XANTHATE 4181-05-9 4-Formyltriphenylamine 25035-71-6 POLY(P-TOLUENESULFONAMIDE-CO-FORMALDEHYDE) 7440-50-8 COPPER 35NM 7440-50-8 COPPER 80NM 7440-50-8 COPPER 100NM 7440-48-4 COBALT 35NM 7440-48-4 COBALT 80NM 7440-48-4 COBALT 100NM 7440-02-0 NICKEL 35NM 7440-02-0 NICKEL 80NM 7440-02-0 NICKEL 100NM 7440-66-6 ZINC 35NM 7440-66-6 ZINC 80NM 7440-66-6 ZINC 100NM 7440-22-4 SILVER 35NM 7440-22-4 SILVER 80NM 7440-22-4 SILVER 100NM 7440-22-4 SILVER NANOPOWDER 150nm 5470-18-8 2-Chloro-3-nitropyridin 123-25-1 DIETHYL SUCCINATE 7206-70-4 4-amino-5-chloro-2-methoxybenzoic acid 109384-19-2 1-Boc-4-hydroxypiperidine 504-02-9 1,3-cyclohexanedione 59-88-1 phenylhydrazine HCl 79-21-0 Peracetic acid solution Mg+Er ナミキ商事株式会社 医薬化学品部 TEL:03-3354-4026/FAX:03-3352-2196/E-mail:[email protected] Intermediates-3 2/89 page CAS Chemical Name Mg+Ho Mg+Tm Mg+Tb Mg+Dy Mg+Gd 87392-07-2 (S)-(-)-Tetrahydro-2-furoic acid 7783-47-3 Tin (II) fluoride 111-36-4 Butyl Isocyanate 112-96-9 Octadecyl Isocyanate 132-98-9 Penicillin V potassium salt 90-98-2 4,4`-dichlorobenzophenone 8006-64-2 Turpentine oil 620-23-5 m-Tolualdehyde 22199-08-2 Silver sulfadiazine 2142-70-3 2'-IODOACETOPHENONE 77-52-1 Ursolic -
Fire Diamond HFRS Ratings
Fire Diamond HFRS Ratings CHEMICAL H F R Special Hazards 1-aminobenzotriazole 0 1 0 1-butanol 2 3 0 1-heptanesulfonic acid sodium salt not hazardous 1-methylimidazole 3 2 1 corrosive, toxic 1-methyl-2pyrrolidinone, anhydrous 2 2 1 1-propanol 2 3 0 1-pyrrolidinecarbonyl chloride 3 1 0 1,1,1,3,3,3-Hexamethyldisilazane 98% 332corrosive 1,2-dibromo-3-chloropropane regulated carcinogen 1,2-dichloroethane 1 3 1 1,2-dimethoxyethane 1 3 0 peroxide former 1,2-propanediol 0 1 0 1,2,3-heptanetriol 2 0 0 1,2,4-trichlorobenzene 2 1 0 1,3-butadiene regulated carcinogen 1,4-diazabicyclo(2,2,2)octane 2 2 1 flammable 1,4-dioxane 2 3 1 carcinogen, may form explosive mixture in air 1,6-hexanediol 1 0 0 1,10-phenanthroline 2 1 0 2-acetylaminofluorene regulated carcinogen, skin absorbtion 2-aminobenzamide 1 1 1 2-aminoethanol 3 2 0 corrosive 2-Aminoethylisothiouronium bromide hydrobromide 2 0 0 2-aminopurine 1 0 0 2-amino-2-methyl-1,3-propanediol 0 1 1 2-chloroaniline (4,4-methylenebis) regulated carcinogen 2-deoxycytidine 1 0 1 2-dimethylaminoethanol 2-ethoxyethanol 2 2 0 reproductive hazard, skin absorption 2-hydroxyoctanoic acid 2 0 0 2-mercaptonethanol 3 2 1 2-Mercaptoethylamine 201 2-methoxyethanol 121may form peroxides 2-methoxyethyl ether 121may form peroxides 2-methylamino ethanol 320corrosive, poss.sensitizer 2-methyl-3-buten-2-ol 2 3 0 2-methylbutane 1 4 0 2-napthyl methyl ketone 111 2-nitrobenzoic acid 2 0 0 2-nitrofluorene 0 0 1 2-nitrophenol 2 1 1 2-propanol 1 3 0 2-thenoyltrifluoroacetone 1 1 1 2,2-oxydiethanol 1 1 0 2,2,2-tribromoethanol 2 0 -
The Chemical Synthesis of the Crinine and Haemanthamine
molecules Review The Chemical Synthesis of the Crinine and Haemanthamine Alkaloids: Biologically Active and Enantiomerically-Related Systems that Serve as Vehicles for Showcasing New Methodologies for Molecular Assembly † Nan Hu, Lorenzo V. White, Ping Lan and Martin G. Banwell * Institute for Advanced and Applied Chemical Synthesis, Jinan University, Zhuhai 519070, China; [email protected] (N.H.); [email protected] (L.V.W.); [email protected] (P.L.) * Correspondence: [email protected] † Dedicated to the memory of our friend and colleague Professor Lew Mander FAA, FRS (1939–2020). Abstract: The title alkaloids, often referred to collectively as crinines, are a prominent group of structurally distinct natural products with additional members being reported on a regular basis. As such, and because of their often notable biological properties, they have attracted attention as synthetic targets since the mid-1950s. Such efforts continue unabated and more recent studies on these alkaloids have focused on using them as vehicles for showcasing the utility of new synthetic methods. This review provides a comprehensive survey of the nearly seventy-year history of these Citation: Hu, N.; White, L.V.; Lan, P.; synthetic endeavors. Banwell, M.G. The Chemical Synthesis of the Crinine and Keywords: alkaloid; crinine; haemanthamine Haemanthamine Alkaloids: Biologically Active and Enantiomerically-Related Systems that Serve as Vehicles for Showcasing 1. Introduction New Methodologies for Molecular The alkaloids isolated from the widely distributed herbaceous and bulbous flowering Assembly . Molecules 2021, 26, 765. plants of the amaryllis (Amaryllidaceae) family number more than five hundred and about https://doi.org/10.3390/molecules 10% of these embody the 2,3,4,4a-tetrahydro-1H,6H-5,10b-ethanophenanthridine ring sys- 26030765 tem (Figure1)[ 1]. -
An Investigation of the Protein Metabolism in Healthy
AN INVESTIGATION OF THE PROTEIN METABOLISM IN HEALTHY SUBJECTS AND WEIGHT-LOSING CANCER PATIENTS by DONALD CAMPBELL McMILLAN F.I.M.L.S. A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY to THE UNIVERSITY OF GLASGOW from RESEARCH CONDUCTED IN THE UNIVERSITY DEPARTMENT OF SURGERY, GLASGOW ROYAL INFIRMARY MARCH 1992 © DONALD C. McMILLAN 1 ProQuest Number: 11007691 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a com plete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest 11007691 Published by ProQuest LLC(2018). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States C ode Microform Edition © ProQuest LLC. ProQuest LLC. 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106- 1346 Glasgow UNIVERSITY LIBRARY 9 iia , 1 CONTENTS PAGE Contents 2 List of Tables 8 List of Figures 13 Acknowledgements 15 Declaration 17 Dedication 20 Summary 21 1. INTRODUCTION AND AIMS 1.1 Protein metabolism in man 24 1.2 Turnover measurements using isotope tracers 27 1.3 Measurement of stable isotopes 29 1.4 Protein turnover measurements 29 1.5 Cancer cachexia 1.5.1 Weight loss in cancer (cancer cachexia) 30 1.5.2 Body composition in cancer cachexia 32 1.5.3 Energy metabolism in cancer cachexia 33 1.5.4 Whole body protein metabolism in cancer cachexia 34 1.5.5 Tissue protein metabolism in cancer cachexia 36 1.6 Aims of the thesis 39 1.7 Plan of thesis 39 2 PAGE 2. -
DDT-10-00042-OA Proof.Indd
349 Drug Discoveries & Therapeutics. 2010; 4(5):349-354. Original Article Evaluation of therapeutic effects and pharmacokinetics of antibacterial chromogenic agents in a silkworm model of Staphylococcus aureus infection Tomoko Fujiyuki, Katsutoshi Imamura, Hiroshi Hamamoto, Kazuhisa Sekimizu* Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan. ABSTRACT: The therapeutic effect of dye diseases, chemical compounds with antibacterial compounds with antibacterial activity was evaluated activity in vitro are tested for their therapeutic in a silkworm model of Staphylococcus aureus efficacy in vivo in animal infection models. A serious infection. Among 13 chromogenic agents that show problem is that most of compounds that exhibit antibacterial activity against S. aureus (MIC = 0.02 antibacterial activity in vitro do not have therapeutic to 19 μg/mL), rifampicin had a therapeutic effect. effects in animal infection models due to toxicity The ED50 value in the silkworm model was consistent and pharmacokinetic issues. Thus, for efficient drug with that in a murine model. Other 12 dyes did not discovery, protocols must be established to exclude increase survival of the infected silkworms. We agents without therapeutic effects at earlier stages of examined the reason for the lack of therapeutic drug development. Evaluation of the therapeutic effects efficacy. Amidol, pyronin G, and safranin were of potential antibiotics has been performed using toxic to silkworms, which explained the lack of mammalian models, but conventional methods using a therapeutic effects. Fuchsin basic and methyl green large number of mammals are problematic due to high disappeared quickly from the hemolymph after costs and ethical concerns. Therefore, the development injection, suggesting that they are not stable in the of a non-vertebrate infection model to test drug efficacy hemolymph. -
Kinetics and Regulation of Mitochondrial Cation Transport Systems
Kinetics and Regulation of Mitochondrial Cation Transport Systems Martin Jaburek B.S., Palacky University, 1992 A dissertation submitted to the faculty of the Oregon Graduate Institute of Science and Technology in partial fulfillment of the requirements for the degree Doctor of Philosophy III Biochemistry and Molecular Biology August 1999 The dissertation "Kinetics and Regulation of Mitochondrial Cation Transport Systems" by Martin Jaburek has been examined and approved by the following Examination Committee: Keith D. Garlid, Advisor Professor Gebre W~ldegi9rgis Associate Proressor /U_- /Peter A. Zuber Professor ~. -Jft~.rofessorKaple:m Oregon Health Sciences University 11 DEDICATION I would like to dedicate this thesis to my wife, Iva, my daughter, Veronika, and also to my parents, grandparents, and all the members of my large family, as an excuse for not being with them for such a long time. iii ACKNOWLEDGMENTS I would like to extend my gratitude to my major advisor, Dr. Keith Garlid. I am very fortunate to have had the opportunity to be a student in his laboratory, and I am very thankful for his support, guidance, and encouragement, without which I would not have been able to finish this degree. I would further like to thank Dr. Gebre Woldegiorgis for the many thought- provoking and valuable discussions we had. Thanks also to Drs. Peter Zuber and Jack Kaplan for their time and for serving as members of my thesis committee. My further thanks to Dr. Petr Paucek, who introduced me to the secrets of reconstitution; to Dr. Martin Modriansky, for being such a good roommate and for sharing the secrets of yeast expression of uncoupling proteins; to Dr.