MRPL24 Antibody Cat. No.: 29-467

MRPL24 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human MRPL24.

TESTED APPLICATIONS: ELISA, WB

MRPL24 antibody can be used for detection of MRPL24 by ELISA at 1:62500. MRPL24 APPLICATIONS: antibody can be used for detection of MRPL24 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) 721_B Cell Lysate

PREDICTED MOLECULAR 25 kDa WEIGHT:

Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

September 26, 2021 1 https://www.prosci-inc.com/mrpl24-antibody-29-467.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: MRPL24 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: MRPL24

ALTERNATE NAMES: MRPL24, FLJ20917, MGC22737, MGC9831, MRP-L18, L24mt, MRP-L24

ACCESSION NO.: NP_078816

PROTEIN GI NO.: 22035588

GENE ID: 79590

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Mammalian mitochondrial ribosomal are encoded by nuclear and help in synthesis within the . Mitochondrial (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by . MRPL24 is a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24).Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and BACKGROUND: help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This encodes a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24). Sequence analysis identified two transcript variants that encode the same protein.

REFERENCES: 1) Gregory, S.G., (2006) Nature 441 (7091), 315-321.

ANTIBODIES FOR RESEARCH USE ONLY.

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