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Supplementary Dataset S2

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mitochondrial translational termination MRPL28 MRPS26 6 MRPS21 PTCD3 MTRF1L 4 MRPL50 MRPS18A MRPS17 2 MRPL20 MRPL52 0 MRPL17 MRPS33 MRPS15 −2 MRPL45 MRPL30 MRPS27 AURKAIP1 MRPL18 MRPL3 MRPS6 MRPS18B MRPL41 MRPS2 MRPL34 GADD45GIP1 ERAL1 MRPL37 MRPS10 MRPL42 MRPL19 MRPS35 MRPL9 MRPL24 MRPS5 MRPL44 MRPS23 MRPS25 ITB ITB ITB ITB ICa ICr ITL original ICr ICa ITL ICa ITL original ICr ITL ICr ICa mitochondrial translational elongation MRPL28 MRPS26 6 MRPS21 PTCD3 MRPS18A 4 MRPS17 MRPL20 2 MRPS15 MRPL45 MRPL52 0 MRPS33 MRPL30 −2 MRPS27 AURKAIP1 MRPS10 MRPL42 MRPL19 MRPL18 MRPL3 MRPS6 MRPL24 MRPS35 MRPL9 MRPS18B MRPL41 MRPS2 MRPL34 MRPS5 MRPL44 MRPS23 MRPS25 MRPL50 MRPL17 GADD45GIP1 ERAL1 MRPL37 ITB ITB ITB ITB ICa ICr original ICr ITL ICa ITL ICa ITL original ICr ITL ICr ICa translational termination MRPL28 MRPS26 6 MRPS21 PTCD3 C12orf65 4 MTRF1L MRPL50 MRPS18A 2 MRPS17 MRPL20 0 MRPL52 MRPL17 MRPS33 −2 MRPS15 MRPL45 MRPL30 MRPS27 AURKAIP1 MRPL18 MRPL3 MRPS6 MRPS18B MRPL41 MRPS2 MRPL34 GADD45GIP1 ERAL1 MRPL37 MRPS10 MRPL42 MRPL19 MRPS35 MRPL9 MRPL24 MRPS5 MRPL44 MRPS23 MRPS25 ITB ITB ITB ITB ICa ICr original ICr ITL ICa ITL ICa ITL original ICr ITL ICr ICa translational elongation DIO2 MRPS18B MRPL41 6 MRPS2 MRPL34 GADD45GIP1 4 ERAL1 MRPL37 2 MRPS10 MRPL42 MRPL19 0 MRPL30 MRPS27 AURKAIP1 −2 MRPL18 MRPL3 MRPS6 MRPS35 MRPL9 EEF2K MRPL50 MRPS5 MRPL44 MRPS23 MRPS25 MRPL24 MRPS33 MRPL52 EIF5A2 MRPL17 SECISBP2 MRPS15 MRPL45 MRPS18A MRPS17 MRPL20 MRPL28 MRPS26 MRPS21 PTCD3 ITB ITB ITB ITB ICa ICr ICr ITL original ITL ICa ICa ITL ICr ICr ICa original ITL cellular protein complex disassembly MRPL28 MRPS26 6 MRPS21 PTCD3 MTRF1L 4 MRPL50 MRPL17 2 DNAJC6 MRPL20 MRPS18A 0 MRPS33 MRPL52 −2 STMN3 MRPL30 MRPS27 AURKAIP1 MRPL3 MRPS6 MRPS10 MRPL42 MRPL19 MRPL9 MRPL24 GADD45GIP1 VPS4B MRPS23 MRPS25 MRPL44 MRPS35 CCSAP ERAL1 MRPL37 MRPS18B MRPL41 MRPS2 MRPL34 ITB ITB ITB ITB ICa ITL ICa ICa ICr original ICr ITL ITL ITL ICr ICa original ICr protein complex disassembly MRPL28 MRPS26 6 MRPS21 PTCD3 MTRF1L 4 MRPL50 MRPL17 DNAJC6 2 MRPL20 MRPS18A 0 MRPS33 MRPL52 STMN3 −2 MRPL30 MRPS27 AURKAIP1 MRPL3 MRPS6 MRPS10 MRPL42 MRPL19 MRPL9 MRPL24 GADD45GIP1 CHMP5 VPS4B MRPS23 MRPS25 MRPL44 MRPS35 CCSAP ERAL1 MRPL37 MRPS18B MRPL41 MRPS2 MRPL34 ITB ITB ITB ITB ICa ITL ICa ICa ICr original ICr ITL ITL ICr ICr ICa original ITL 10 8 6 4 2 0 −2 ACTG2 ACTA2 ACTA1 ACTC1 ICr original ICr ICa ITL ICa ITL original ICr ICa ICr ITL mesenchyme migration mesenchyme ITL ITB ICa ITB ITB ITB 10 8 6 4 2 0 −2 ACTG2 ACTA2 ACTA1 ACTC1 ICr original ICr ICa ITL ICa ITL original ICr ICa ICr tissue migration ITL ITL ITB ICa ITB ITB ITB macromolecular complex disassembly HIST3H2A 8 MRPL28 MRPS26 MRPS21 6 PTCD3 C12orf65 MTRF1L 4 MRPL50 MRPL17 DNAJC6 2 MRPS18A MRPS17 MRPL20 0 MRPS15 MRPL45 MRPS33 −2 MRPL52 STMN3 MRPL30 MRPS27 AURKAIP1 MRPS10 MRPL42 MRPL19 MRPL18 MRPL3 MRPS6 MRPS35 CCSAP ERAL1 MRPL37 GADD45GIP1 VTA1 CHMP5 VPS4B MRPS18B MRPL41 MRPS2 MRPL34 MRPL9 MRPL24 MRPS5 MRPL44 MRPS23 MRPS25 ITB ITB ITB ITB ICa ICr original ICr ICa ICa ITL ITL ITL original ITL ICr ICr ICa 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB ITB ITB ITB secretion by lung epithelial cell involved in lung growth lung epithelial cell involved secretion by ICa cell division FGFR2 PARD6A ZWILCH 8 HAUS4 MAD2L2 ZFYVE19 DYNLT3 6 TERF1 KNSTRN STRA13 CENPV 4 CENPJ CTDP1 CDCA2 CDC25C CENPE 2 C9orf114 RGS14 SIRT2 PMF1 0 CABLES2 SPICE1 PARD6B AURKA −2 CENPF CDC25B GOLGA2 TACC3 CDCA8 ANKLE2 CDCA5 BIRC5 CKS1B DSN1 REEP3 ANAPC13 PSRC1 FBXO5 BUB1 ZWINT CDCA3 SPDL1 NCAPG2 SKA1 BUB1B SPECC1L CDK1 SKA2 MIS18BP1 NCAPH KIF14 KIFC1 NSL1 PTTG1 HAUS5 SPG20 KIF20B CCSAP BABAM1 HAUS2 NEK2 DYNLT1 ANAPC16 MIS12 TIMELESS SDCCAG3 SGOL2 NUP43 ASPM HAUS6 ITB ITB ITB ITB ICa ITL ITL ICr original ICr ICa ICr ITL ITL original ICr ICa ICa 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB ITB ITB ITB ICa regulation of branching involved in salivary gland morphogenesis by mesenchymal−epithelial signaling mesenchymal−epithelial gland morphogenesis in salivary by involved regulation of branching 8 6 4 2 0 −2 WNT2 GJA4 HTR1B DSG2 HILPDA FGF7 BDNF SLC1A6 NGF FGF10 ICa ICr ICr ITL ICr ICr original ICa ICa ITL original ITL ITL single organism signaling single organism ITB ITB ITB ITB ICa 6 4 2 0 −2 NOG FGF10 ITL original ITL ITL ICr original ICr ICr ICr ITL ICa ICa prostatic bud formation bud prostatic ICa ITB ITB ITB ITB ICa 8 6 4 2 0 −2 WNT2 GJA4 HTR1B DSG2 HILPDA FGF7 BDNF SLC1A6 NGF FGF10 ICa ICr ICr ITL ICr ICr original ICa ICa ITL signaling original ITL ITL ITB ITB ITB ITB ICa cellular component disassembly MMP16 HIST3H2A 8 POM121 NUP50 NUP43 CAPNS1 6 MRPS27 AURKAIP1 MRPL30 4 GOLGA2 CTSL MRPS10 2 GADD45GIP1 CHMP5 VPS4B MRPS5 0 MRPL44 MRPS23 MRPS25 −2 MRPS2 MRPL34 MRPL19 MRPL42 CDK1 MRPL3 MRPS6 LPIN1 MRPS35 CCSAP ERAL1 MRPL37 C12orf65 MRPS18B MRPL41 MRPL9 MRPL24 NUP210 WASH1 STMN3 MRPL28 FIS1 MRPS26 SHARPIN MRPS21 PTCD3 MRPL20 MRPS18A MRPS33 FUNDC2 MRPL50 NUPL2 MTRF1L MRPL52 MRPL17 DNAJC6 ITB ITB ITB ITB ICa ICr original ICr ICa ICa ITL ITL original ITL ITL ICr ICr ICa 8 6 4 2 0 −2 WNT2 FGF7 NOG FGF10 ITL original ITL ITL ICr ICr ITL ICa ICr ICr ICa ICa original ITB ITB ITB ITB regulation of morphogenesis structure of a branching ICa 8 6 4 2 0 −2 WNT2 FGF7 FGFR2 ICa ICr ICr ICa original ITL ICr ICr ICa ITL ITL ITL original ITB ITB ITB ITB ICa positive regulation of epithelial cell proliferation involved in lung morphogenesis involved positive regulation of epithelial cell proliferation mitotic nuclear division AURKA CENPF 8 CDC25B CDCA5 ANKLE2 6 ANAPC16 NUP43 TIMELESS 4 STRA13 DYNLT3 ZWILCH 2 PBK MAD2L2 HAUS4 0 CDCA2 CENPA CDC25C −2 PMF1 RGS14 SIRT2 DSN1 REEP3 ANAPC13 ASPM HAUS6 FBXO5 BUB1 CENPN KIF20B CCSAP BABAM1 HAUS2 NEK2 DYNLT1 NCAPG2 SKA1 BUB1B KIF22 MIS18BP1 SKA2 CDK1 HAUS5 CDCA3 PTTG1 CENPV TERF1 KLHL9 KLHL13 ITB ITB ITB ITB ICa ITL ITL ICr original ICr ICa ICa ICa ITL ITL ICr original ICr 6 4 2 0 −2 NOG FGF10 ITL original ITL ITL ICr original ICr ICr ICr ITL ICa ICa ICa ITB morphogenesis of an epithelial bud ITB ITB ITB ICa 8 6 4 2 0 −2 WNT2 GJA4 HTR1B HILPDA FGF7 BDNF SLC1A6 NGF FGF10 ICa ICr ICr ITL ICr ICr original ICa ICa ITL original cell−cell signaling ITL ITL ITB ITB ITB ITB ICa 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB ITB ITB positive regulation of keratinocyte proliferation positive regulation of keratinocyte ITB ICa 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB ITB positive regulation of keratinocyte migration positive regulation of keratinocyte ITB ITB ICa protein−DNA complex assembly POLR1E 8 HJURP 6 GTF3C5 SART3 4 GTF2H3 2 ASF1B 0 XRCC3 MIS18BP1 −2 TAF11 HIST2H2BF HIST1H2BN HIST3H2BB DACH1 HIST1H2BI HIST1H2BL HIST1H2BH HIST1H4K HIST1H4J HIST2H3D HIST1H4I HIST1H2BE HIST1H2BO ITL ITL ITL ICa ICa ICr original ICr ITL ICr ICr original ICa ICa ITB ITB ITB ITB 8 6 4 2 0 −2 WNT2 FGF7 FGFR2 ICa ICr ICr ICa original ITL ICr ICr ICa ITL ITL ITL original ITB ITB ITB ITB ICa regulation of epithelial cell proliferation involved in lung morphogenesis involved regulation of epithelial cell proliferation 6 4 2 0 −2 FGF10 WNT2 FOXC2 NOG FGFR2 SLIT2 GJA4 TFAP2B ITL original ICr ITL ICa ICr ITL ICr ICr ICa original ICa epithelium development ITL ITB ITB ITB ICa ITB 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB regulation of keratinocyte migration regulation of keratinocyte ITB ITB ITB ICa 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB mesenchymal−epithelial cell signaling mesenchymal−epithelial ITB ITB ITB ICa 6 4 2 0 −2 NOG FGFR2 FGF10 ICa ITL ICr ICr ITL original ITL ICr ICr ICa ICa original ITL ITB lateral sprouting from an epithelium from lateral sprouting ITB ITB ITB ICa 8 6 4 2 0 −2 WNT2 GJA4 HTR1B DAP HILPDA FGF7 BDNF SLC1A6 NGF FGF10 ITB ITB ITB ITB ICa ICa ICr ICr ITL ICr ICr original cell communication ICa ICa ITL original ITL ITL 8 6 4 2 0 −2 FGF10 FGF7 ITL original ITL ITL ICa ICr ICa ICr ICr ICr ICa ITL original ITB ITB ITB ITB ICa regulation of branching involved in salivary gland morphogenesis in salivary involved regulation of branching 6 4 2 0 −2 FGFR2 FGF10 ICr original ICr ICa ICa ITL ITL ICr ICa ITL ICr original ITL ITB ITB ITB ITB ICa mesenchymal cell differentiation involved in lung development involved cell differentiation mesenchymal 6 4 2 0 −2 FGFR2 FGF10 ICr original ICr ICa ICa ITL ITL ICr ICa ITL ICr original ITL ITB ITB ITB ITB ICa branch elongation involved in salivary gland morphogenesis in salivary elongation involved branch 6 4 2 0 −2 FGFR2 FGF10 ICr original ICr ICa ICa ITL ITL ICr ICa ITL ICr original ITL mammary gland bud formation mammary gland bud ITB ITB ITB ITB ICa 6 4 2 0 −2 FGFR2 FGF10 ICr original ICr ICa ICa ITL ITL ICr ICa ITL ICr original ITL ITB ITB ITB ITB ICa fibroblast growth factor receptor signaling pathway involved in mammary gland specification involved factor receptor signaling pathway growth fibroblast defense response to virus BCL2 MICA 8 TICAM1 APOBEC3C 6 PLSCR1 4 IFIT5 IL10RB 2 ISG15 IFIT3 0 IRF3 FADD −2 SLFN11 IFNAR2 TRIM5 POLR3G GBP3 PML SERINC5 IFNGR1 SERINC3 TRIM56 IRF1 STAT2 TRIM25 ZC3HAV1 PMAIP1 MAVS IFITM3 IFI16 IFNAR1 FAM111A ITB ITB ITB ITB ICa ICr original ICr ITL ITL ICa ICa ITL original ICr ICr ITL ICa 6 4 2 0 −2 NOG WNT2 ITL ICr ICr ICr ICr ICa ICa ICa ITL original ITL original ITL ITB ITB atrial cardiac muscle tissue morphogenesis muscle atrial cardiac ICa ITB ITB 8 6 4 2 0 −2 SC5D EBP DHCR7 ICr ICr ITL ICa ITL original
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  • 1 AGING Supplementary Table 2

    1 AGING Supplementary Table 2

    SUPPLEMENTARY TABLES Supplementary Table 1. Details of the eight domain chains of KIAA0101. Serial IDENTITY MAX IN COMP- INTERFACE ID POSITION RESOLUTION EXPERIMENT TYPE number START STOP SCORE IDENTITY LEX WITH CAVITY A 4D2G_D 52 - 69 52 69 100 100 2.65 Å PCNA X-RAY DIFFRACTION √ B 4D2G_E 52 - 69 52 69 100 100 2.65 Å PCNA X-RAY DIFFRACTION √ C 6EHT_D 52 - 71 52 71 100 100 3.2Å PCNA X-RAY DIFFRACTION √ D 6EHT_E 52 - 71 52 71 100 100 3.2Å PCNA X-RAY DIFFRACTION √ E 6GWS_D 41-72 41 72 100 100 3.2Å PCNA X-RAY DIFFRACTION √ F 6GWS_E 41-72 41 72 100 100 2.9Å PCNA X-RAY DIFFRACTION √ G 6GWS_F 41-72 41 72 100 100 2.9Å PCNA X-RAY DIFFRACTION √ H 6IIW_B 2-11 2 11 100 100 1.699Å UHRF1 X-RAY DIFFRACTION √ www.aging-us.com 1 AGING Supplementary Table 2. Significantly enriched gene ontology (GO) annotations (cellular components) of KIAA0101 in lung adenocarcinoma (LinkedOmics). Leading Description FDR Leading Edge Gene EdgeNum RAD51, SPC25, CCNB1, BIRC5, NCAPG, ZWINT, MAD2L1, SKA3, NUF2, BUB1B, CENPA, SKA1, AURKB, NEK2, CENPW, HJURP, NDC80, CDCA5, NCAPH, BUB1, ZWILCH, CENPK, KIF2C, AURKA, CENPN, TOP2A, CENPM, PLK1, ERCC6L, CDT1, CHEK1, SPAG5, CENPH, condensed 66 0 SPC24, NUP37, BLM, CENPE, BUB3, CDK2, FANCD2, CENPO, CENPF, BRCA1, DSN1, chromosome MKI67, NCAPG2, H2AFX, HMGB2, SUV39H1, CBX3, TUBG1, KNTC1, PPP1CC, SMC2, BANF1, NCAPD2, SKA2, NUP107, BRCA2, NUP85, ITGB3BP, SYCE2, TOPBP1, DMC1, SMC4, INCENP. RAD51, OIP5, CDK1, SPC25, CCNB1, BIRC5, NCAPG, ZWINT, MAD2L1, SKA3, NUF2, BUB1B, CENPA, SKA1, AURKB, NEK2, ESCO2, CENPW, HJURP, TTK, NDC80, CDCA5, BUB1, ZWILCH, CENPK, KIF2C, AURKA, DSCC1, CENPN, CDCA8, CENPM, PLK1, MCM6, ERCC6L, CDT1, HELLS, CHEK1, SPAG5, CENPH, PCNA, SPC24, CENPI, NUP37, FEN1, chromosomal 94 0 CENPL, BLM, KIF18A, CENPE, MCM4, BUB3, SUV39H2, MCM2, CDK2, PIF1, DNA2, region CENPO, CENPF, CHEK2, DSN1, H2AFX, MCM7, SUV39H1, MTBP, CBX3, RECQL4, KNTC1, PPP1CC, CENPP, CENPQ, PTGES3, NCAPD2, DYNLL1, SKA2, HAT1, NUP107, MCM5, MCM3, MSH2, BRCA2, NUP85, SSB, ITGB3BP, DMC1, INCENP, THOC3, XPO1, APEX1, XRCC5, KIF22, DCLRE1A, SEH1L, XRCC3, NSMCE2, RAD21.
  • Knockdown of EIF5A2 Inhibits the Malignant Potential of Non-Small Cell Lung Cancer Cells

    Knockdown of EIF5A2 Inhibits the Malignant Potential of Non-Small Cell Lung Cancer Cells

    ONCOLOGY LETTERS 15: 4541-4549, 2018 Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells CHENG CHEN1*, BOJIA ZHANG2*, SHANSHAN WU3, YONGXIANG SONG1 and JIAN LI1 1Department of Thoracic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000; 2Department of Infectious Disease, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072; 3Department of Nursing, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China Received March 16, 2016; Accepted February 23, 2017 DOI: 10.3892/ol.2018.7832 Abstract. Eukaryotic translation initiation factor 5A2 through these proteins. EIF5A2 may therefore serve as a novel (EIF5A2) has been demonstrated to be upregulated in therapeutic target for the treatment of NSCLC. numerous types of human cancer and is associated with cancer progression. However, the expression and role of Introduction EIF5A2 in non-small cell lung cancer (NSCLC) remains unclear. In the present study, the role of EIF5A2 in NSCLC Non-small cell lung cancer (NSCLC) accounts for 85% of was investigated, in addition to the underlying molecular pulmonary malignancies (1) and is one of the leading causes of mechanisms by which EIF5A2 acts. Relative EIF5A2 expres- cancer-associated mortality worldwide. Recurrent and meta- sion levels were determined in NSCLC cells and compared static disease is the main cause of mortality, and the 5-year with levels in non-cancerous lung tissues. Short interfering survival rate for lung cancer is 15% (2). Therefore, in order to (si)RNA targeted against EIF5A2 was used to knock down improve the prognosis for patients with NSCLC, it is impor- EIF5A2 levels in NSCLC cells.
  • WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT

    WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT

    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization I International Bureau (10) International Publication Number (43) International Publication Date WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT (51) International Patent Classification: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, C12Q 1/68 (2018.01) A61P 31/18 (2006.01) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C12Q 1/70 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (21) International Application Number: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PCT/US2018/056167 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 16 October 2018 (16. 10.2018) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 62/573,025 16 October 2017 (16. 10.2017) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, ΓΕ , IS, IT, LT, LU, LV, (71) Applicant: MASSACHUSETTS INSTITUTE OF MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TECHNOLOGY [US/US]; 77 Massachusetts Avenue, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Cambridge, Massachusetts 02139 (US).