Pharmacological Studies on Three Sudanese Medicinal Plants Possessing Antimicrobial Properties
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Pharmacological Studies on three Sudanese Medicinal Plants Possessing Antimicrobial Properties By: Mohana Mohamed El-Hassan Gadour (B.Pharm.) A thesis submitted to the university of Khartoum in fulfillment of the requirements for the master degree, at the department of pharmacology, faculty of pharmacy. Under Supervision of: Professor Idris Babekir Eltayeb November 2004 Dedication To my parents, The one who teach a lot now get some part. To my brothers, The one who work for others will get some part. To my children’s, When asking for knowledge try to get all and not part. ii Acknowledgements I was and remain even at the conclusion of this work indebted for my supervisor Professor Idris Babekir Eltayeb of the department of pharmacology, faculty of pharmacy, University of Khartoum for his guidance and motivating criticism. I am extremely grateful for his wisdom, encourage and patience. My deep appreciation is due to Dr. Abd ElWahab Hassan Mohamed, who initiated this work and skilled me with some of the techniques he had. I am in even more in awe of his valuable help. I would like to express my gratitude to the Department of Pharmacology, Faculty of pharmacy, University of Khartoum for availing this chance for me. I am particularly grateful to the pharmacology and toxicology department of MAPRI and its generous staff. iii List of Contents PAGE Dedication............................................................................................... I Acknowledgements................................................................................. II List of Contents:...................................................................................... III List of Figures:........................................................................................ VIII Abstract in Arabic................................................................................... X Abstract in English.................................................................................. XII CHAPTER ONE 1 Introduction....................................................................................... 1 1.1 Sudan location and vegetations diversity ......................................... 1 1.2 medicinal plants as potential source of antimicrobial agent:............ 1 1.3 drugs- herbs interaction: ................................................................... 4 1.4 proposed alternatives: ....................................................................... 6 1.5 Family Loranthacae taxonomy ...................................................... 6 1.5.1 Loranthus acacia: ..................................................................... 7 1.5.2 Folkloric use: ............................................................................ 7 1.5.3 Economic importance:.............................................................. 9 1.5.4 Chemical constituents:.............................................................. 9 1.5.5 Biological activity:.................................................................... 10 1.5.6 Anticholinestrase activity in plant kingdom:............................ 11 1.6 Anticholinesterases:.......................................................................... 16 1.6.1 Short –acting anticholinesterase:.............................................. 16 1.6.2 Medium –duration anticholinesterases:.................................... 17 1.6.3 Irreversible anticholinesterases:................................................ 17 1.6.4 Clinical uses of anticholinesterases:......................................... 1 1.7 Family Nymphaeaceae taxonomy: ................................................... 19 1.7.1 Nymphaea lotus L.:................................................................... 19 1.7.2 Folkloric uses:........................................................................... 20 1.7.3 Chemical constituents:.............................................................. 20 1.7.4 Biological activity: ................................................................... 24 1.7.5 Serotonin occurrence in plant kingdom:................................... 24 1.8 5- hydroxytryptamine : ................................................................... 27 1.8.1 5-Hydroxytryptamine Distribution, biosynthesis and degradation: ............................................................................. 27 1.8.2 Actions and functions of 5-HT: ............................................. 27 1.8.3 Postulated physiological and pathophysiological roles ............ includes: .................................................................................... 28 1.8.4 5-HT receptor:........................................................................... 29 iv 1.8.5 Important drugs that act on 5-HT receptors in the periphery:.. 30 1.9 Family Combretaceae taxonomy: .................................................... 31 1.9.1 Anogeissus leiocarpus : ............................................................ 31 1.9.2 Economic value: ....................................................................... 32 1.9.3 Chemical constituents:.............................................................. 32 1.9.4 Biological activity: ................................................................... 35 1.10 Classification of adrenoceptors : .................................................... 36 1.11 Clinical uses of adrenceptor agonists : ........................................... 38 1.11.1 Cardiovascular system: .......................................................... 38 1.11.2 Anaphylactic reactions: ........................................................ 39 1.11.3 Respiratory system: ................................................................ 39 1.11.4 Miscellaneous indications: ..................................................... 40 CHAPTER TWO 2 Materials and Methods: ....................................................................... 41 2.1 Materials: .................................................................................... 41 2.1.1 Plant material: ....................................................................... 41 2.1.2 Chemicals: ............................................................................ 41 2.1.2.1 Physiological Satls: ................................................................. 41 2.1.2.2 Solvents: ....................................................................... 42 2.1.2.3 Standard drugs: ............................................................. 42 2.1.3 Apparatus:............................................................................... 43 2.1.4 Laboratory Animals:............................................................... 43 2.2 Methods: .................................................................................... 44 2.2.1 Method of extraction: ............................................................... 44 2.2.2 Methods of preparation of isolated organs: 44 2.2.2.1 The Guinea-pig ileum preparation: ................................ 44 2.2.2.2 Toad rectus abdominis muscle preparation: ...................... 45 2.2.2.3 The rat fundus strip preparation: ....................................... 46 2.2.2.4 Rabbit jejunum preparation: ............................................ 46 2.2.2.5 The isolated perfused rabbit heart preparation:............... 47 2.2.2.6 Guinea pig atria preparation: .......................................... 47 2.2.2.7 The rat uterus preparation: ............................................. 47 2.2.2.8 Guinea pig tracheal Chain preparation:........................... 48 CHAPTER THREE 3 Results.................................................................................................. 50 Loranthus acacia: .................................................................................. 50 3.1.1 Effect of methanolic extract of Loranthus acacia on isolated... rabbit jejunum preparation: ........................................................ 50 3.1.2 Effect of Adrenoceptor blocking drugs on Loranthus acacia .. extract induced relaxation on isolated rabbit jejunum .............. v preparation:................................................................................. 50 3.1.3 Effect of Loranthus acacia methanolic extract on isolated....... guinea pig ileum preparation: ...................................................... 51 3.1.4 Effect of methanolic extract of Lornthus acacia on isolated .... toad rectus abdominis muscle preparation:................................ 51 3.1.5 Effect of Loranthus acacia methanolic extract on ................... Acetylcholine induced contracture on isolated toad rectus ....... abdominis muscle preparation: .................................................. 51 3.1.6 Loranthus acacia methanolic extract reversal of ...................... tubocurarine antagonistic effect on Acetylcholine ................... induced contracture on isolated Toad rectus ............................... abdominis muscle preparation:..................................................... 52 3.1.7 Effect of mthanolic extract of Loranthus acacia on isolated .... perfused rabbit heart preparation: .............................................. 52 3.1.8 Effect of Loranthus acacia methanolic extract on guinea pig... atrium: ........................................................................................ 52 3.1.9 Potentiation of Loranthus acacia dromotropic effect on ......... isolated guinea pig atrium preparation using Acetylcholine : ... 53 3.1.10 Influence of Atropine sulphate on Loranthuss