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Supporting Information Supporting Information Phytochemicals of Conocarpus spp. as a natural and safe source of phenolic compounds and antioxidants Hanan S. Afifi 1,*, Hassan M. Al Marzooqi 1, Mohammad J. Tabbaa 2,3 and Ahmed A. Arran 2 1 Food Research Section, R&D Division, Abu Dhabi Agriculture and Food Safety Authority, P.O. Box 52150, Abu Dhabi, UAE; [email protected] 2 Agriculture Research Section, R&D Division, Abu Dhabi Agriculture and Food Safety Authority, P.O. Box 52150, Abu Dhabi, UAE; [email protected] (M.J.T.); [email protected] (A.A.A.) 3 Department of Animal Production, School of Agriculture, The University of Jordan, Amman 11942, Jordan; [email protected] (M.J.T.) * Correspondence: [email protected] or [email protected]; Tel.: +97128181759 Abbreviations RSM Response Surface Methodology CCD Central Composite Design CL Conocarpus lancifolius CE Conocarpus erectus CLL Conocarpus lancifolius leaves CLF Conocarpus lancifolius fruits CLR Conocarpus lancifolius roots CEL Conocarpus erectus leaves CEF Conocarpus erectus fruits CER Conocarpus erectus roots Public Table S1. Central composite design arrangement and responses variable of vanillic acid (ppm) at P ≤ 0.05. Standard Order erectus lancifolius Coded variables Leaves Roots Fruits Leaves Roots Fruits Solvent Temp Time (h) conc.% (°C) 1 1 (100) 1 (65) 0(2) 2.54 ± 0.05 22.87 ± 3.89 0.00 ± 0.00 0.00 ± 0.00 15.62 ± 1.23 1.89 ± 0.11 2 −1 (50) 0 (55) 1(3) 0.00 ± 0.00 10.11 ± 0.20 8.64 ± 0.17 0.00 ± 0.00 20.53 ± 0.72 2.36 ± 0.07 3 0 (75) 0 (55) 0(2) 5.00 ± 0.36 16.40 ± 0.93 7.06 ± 0.53 0.01 ± 0.02 19.97 ± 0.16 0.00 ± 0.00 4 0 (75) 1 (65) 1(3) 2.72 ± 0.11 4.45 ± 0.17 5.15 ± 0.26 0.00 ± 0.00 8.04 ± 0.16 1.23 ± 1.10 5 1(100) 0 (55) −1(1) 8.63 ± 0.20 1.786 ± 0.14 2.24 ± 0.05 0.00 ± 0.00 1.27 ± 0.02 0.00 ± 0.00 6 1(100) −1 (45) 0(2) 3.73 ± 0.04 15.40 ± 0.98 4.15 ± 0.19 0.00 ± 0.00 26.40 ± 1.14 0.00 ± 0.00 7 −1(50) 1 (65) 0(2) 8.47 ± 0.21 12.35 ± 0.87 10.78 ± 0.80 0.00 ± 0.00 14.97 ± 0.35 1.46 ± 0.12 8 0 (75) 0 (55) 0(2) 6.22 ± 4.68 18.36 ± 1.00 6.65 ± 0.99 0.00 ± 0.00 15.73 ± 0.63 0.00 ± 0.00 9 0 (75) 1 (65) −1(1) 0.00 ± 0.00 21.60 ± 1.65 4.27 ± 0.25 0.00 ± 0.00 11.99 ± 2.70 0.00 ± 0.00 10 1(100) 0 (55) 1(3) 0.00 ± 0.00 39.37 ± 0.76 12.69 ± 0.27 4.18 ± 0.32 20.26 ± 1.04 0.00 ± 0.00 11 0 (75) −1 (45) 1(3) 5.05 ± 0.06 29.21 ± 0.71 3.50 ± 0.25 3.04 ± 0.27 15.12 ± 0.39 0.00 ± 0.00 12 0 (75) 0 (55) 0(2) 4.00 ± 0.11 16.06 ± 0.30 9.74 ± 0.24 0.00 ± 0.00 15.41 ± 0.57 0.00 ± 0.00 13 −1(50) 0 (55) −1(1) 3.73 ± 0.40 0.00 ± 0.00 2.02 ± 0.03 2.09 ± 0.09 36.57 ± 1.31 0.00 ± 0.00 14 0 (75) −1 (45) −1(1) 0.00 ± 0.00 8.96 ± 0.25 7.57 ± 0.39 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 15 −1(50) −1 (45) 0(2) 3.94 ± 0.19 124.79 ± 3.80 23.17 ± 0.21 7.22 ± 0.47 44.48 ± 2.04 0.00 ± 0.00 Values are expressed as mean ± standard deviation (n = 3). Public Table S2. Central composite design arrangement and responses variable of p-coumaric acid (ppm) at P ≤ 0.05. Standard Order erectus lancifolius Coded variables Leaves Roots Fruits Leaves Roots Fruits Solvent Temp Time (h) conc.% (°C) 1 1 (100) 1 (65) 0(2) 0.02 ± 0.02 3.58 ± 1.24 37.49 ± 4.31 5.36 ± 0.41 2.82 ± 0.12 38.41 ± 1.69 2 −1 (50) 0 (55) 1(3) 4.96 ± 0.06 0.78 ± 0.04 0.00 ± 0.00 74.48 ± 2.87 2.59 ± 0.02 0.00 ± 0.00 3 0 (75) 0 (55) 0(2) 14.74 ± 0.07 0.00 ± 0.00 0.00 ± 0.00 16.68 ± 1.91 4.16 ± 0.14 15.65 ± 0.33 4 0 (75) 1 (65) 1(3) 6.80 ± 2.24 0.00 ± 0.00 0.00 ± 0.00 45.48 ± 11.79 14.97 ± 0.91 27.00 ± 6.30 5 1(100) 0 (55) −1(1) 115.46 ± 2.36 1.76 ± 0.06 9.98 ± 0.87 20.37 ± 1.56 3.00 ± 0.10 21.57 ± 1.75 6 1(100) −1 (45) 0(2) 0.00 ± 0.00 0.84 ± 0.04 0.00 ± 0.00 1.80 ± 0.69 4.16 ± 0.14 4.88 ± 0.13 7 −1(50) 1 (65) 0(2) 3.40 ± 0.01 1.13 ± 0.03 1.11 ± 0.13 44.22 ± 6.74 3.34 ± 0.12 1.03 ± 0.04 8 0 (75) 0 (55) 0(2) 19.90 ± 5.13 0.00 ± 0.00 0.00 ± 0.00 18.81 ± 2.23 3.79 ± 0.13 18.72 ± 0.50 9 0 (75) 1 (65) −1(1) 10.17 ± 0.09 8.97 ± 0.07 0.63 ± 0.05 1.06 ± 0.05 1.20 ± 0.82 0.00 ± 0.00 10 1(100) 0 (55) 1(3) 134.14 ± 1.99 13.61 ± 0.54 42.47 ± 2.32 0.21 ± 0.02 19.79 ± 0.64 7.76 ± 0.25 11 0 (75) −1 (45) 1(3) 12.22 ± 0.74 4.57 ± 0.05 1.37 ± 0.14 2.01 ± 1.81 1.47 ± 0.04 2.56 ± 0.05 12 0 (75) 0 (55) 0(2) 18.46 ± 0.56 0.00 ± 0.00 0.00 ± 0.00 15.05 ± 0.21 3.08 ± 0.09 15.47 ± 1.13 13 −1(50) 0 (55) −1(1) 1.63 ± 0.05 2.25 ± 0.06 0.63 ± 0.03 3.42 ± 1.26 4.93 ± 0.21 10.34 ± 0.79 14 0 (75) −1 (45) −1(1) 1.96 ± 0.36 0.47 ± 0.03 1.90 ± 0.28 0.00 ± 0.00 0.00 ± 0.00 1.01 ± 0.02 15 −1(50) −1 (45) 0(2) 1.95 ± 0.41 235.06 ± 5.00 0.00 ± 0.00 2.59 ± 0.40 46.38 ± 1.40 0.00 ± 0.00 Values are expressed as mean ± standard deviation (n = 3). Public Table S3. Central composite design arrangement and responses variable of t-ferulic acid (ppm) at P ≤ 0.05. Standard Order erectus lancifolius Coded variables Leaves Roots Fruits Leaves Roots Fruits Solvent Temp Time (h) conc.% (°C) 1 1 (100) 1 (65) 0(2) 2.67 ± 0.04 4.14 ± 1.42 0.16 ± 0.15 0.00 ± 0.00 2.55 ± 0.09 0.00 ± 0.00 2 −1 (50) 0 (55) 1(3) 51.80 ± 1.53 0.41 ± 0.01 1.79 ± 0.37 0.00 ± 0.00 3.72 ± 0.18 0.00 ± 0.00 3 0 (75) 0 (55) 0(2) 27.03 ± 0.25 1.22 ± 0.02 1.03 ± 0.06 0.00 ± 0.00 1.40 ± 0.03 0.00 ± 0.00 4 0 (75) 1 (65) 1(3) 19.19 ± 0.17 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.14 ± 0.13 5 1(100) 0 (55) −1(1) 31.86 ± 1.07 1.11 ± 0.02 0.00 ± 0.00 3.96 ± 0.10 0.00 ± 0.00 0.00 ± 0.00 6 1(100) −1 (45) 0(2) 107.45 ± 1.97 0.96 ± 0.14 4.21 ± 0.56 122.75 ± 5.73 6.36 ± 0.06 0.00 ± 0.00 7 −1(50) 1 (65) 0(2) 31.40 ± 0.37 1.20 ± 0.02 1.02 ± 0.03 0.00 ± 0.00 0.80 ± 0.03 1.56 ± 0.08 8 0 (75) 0 (55) 0(2) 23.44 ± 10.50 1.37 ± 0.41 1.38 ± 0.30 0.00 ± 0.00 2.02 ± 0.02 0.00 ± 0.00 9 0 (75) 1 (65) −1(1) 54.44 ± 0.82 1.71 ± 0.06 1.20 ± 0.06 1.09 ± 0.11 0.46 ± 0.04 0.00 ± 0.00 10 1(100) 0 (55) 1(3) 45.33 ± 1.81 1.83 ± 0.08 2.28 ± 0.16 175.20 ± 1.72 8.59 ± 0.35 0.00 ± 0.00 11 0 (75) −1 (45) 1(3) 37.25 ± 3.03 3.03 ± 0.06 0.00 ± 0.00 0.35 ± 0.31 1.08 ± 0.07 1.55 ± 0.10 12 0 (75) 0 (55) 0(2) 27.13 ± 0.27 1.41 ± 0.05 1.00 ± 0.13 0.00 ± 0.00 1.51 ± 0.17 0.00 ± 0.00 13 −1(50) 0 (55) −1(1) 0.84 ± 0.04 0.61 ± 0.02 0.83 ± 0.05 32.01 ± 1.07 1.50 ± 0.17 0.00 ± 0.00 14 0 (75) −1 (45) −1(1) 0.00 ± 0.00 0.00 ± 0.00 1.55 ± 0.32 22.42 ± 2.44 0.00 ± 0.00 1.63 ± 0.09 15 −1(50) −1 (45) 0(2) 1.34 ± 0.08 137.07 ± 1.29 4.10 ± 0.10 37.74 ± 1.07 50.16 ± 1.23 0.00 ± 0.00 Values are expressed as mean ± standard deviation (n = 3).
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