"Polyembryony in Undeveloped Monoembryonic Diploid Seeds Crossed with a Citrus Tetraploid"
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Topic: Apomixis - Definition and Types
Course Teacher: Dr. Rupendra Kumar Jhade, JNKVV, College of Horticulture,Chhindwara(M.P.) B.Sc.(Hons) Horticulture- 1st Year, II Semester 2019-20 Course: Plant Propagation and Nursery Management Lecture No. 12 Topic: Apomixis - Definition and types Apomixis, derived from two Greek word “APO” (away from) and “MIXIS” (act of mixing or mingling). The first discovery of this phenomenon is credited to Leuwen hock as early as 1719 in Citrus seeds. In apomixes, seeds are formed but the embryos develop without fertilization. The genotype of the embryo and resulting plant will be the same as the seed parent. Definition: “The process of development of diploid embryos without fertilization.” Or “Apomixis is a form of asexual reproduction that occurs via seeds, in which embryos develop without fertilization.” Or “Apomixis is a type of reproduction in which sexual organs of related structures take part but seeds are formed without union of gametes.” In some species of plants, an embryo develops from the diploid cells of the seed and not as a result of fertilization between ovule and pollen. This type of reproduction is known as apomixis and the seedlings produced in this manner are known as apomicts. Apomictic seedlings are identical to mother plant and similar to plants raised by other vegetative means, because such plants have the same genetic make-up as that of the mother plant. Such seedlings are completely free from viruses. In apomictic species, sexual reproduction is either suppressed or absent. When sexual reproduction also occurs, the apomixes is termed as facultative apomixis. But when sexual reproduction is absent, it is referred to as obligate apomixis. -
UNIVERSITY of CALIFORNIA RIVERSIDE Cross-Compatibility, Graft-Compatibility, and Phylogenetic Relationships in the Aurantioi
UNIVERSITY OF CALIFORNIA RIVERSIDE Cross-Compatibility, Graft-Compatibility, and Phylogenetic Relationships in the Aurantioideae: New Data From the Balsamocitrinae A Thesis submitted in partial satisfaction of the requirements for the degree of Master of Science in Plant Biology by Toni J Siebert Wooldridge December 2016 Thesis committee: Dr. Norman C. Ellstrand, Chairperson Dr. Timothy J. Close Dr. Robert R. Krueger The Thesis of Toni J Siebert Wooldridge is approved: Committee Chairperson University of California, Riverside ACKNOWLEDGEMENTS I am indebted to many people who have been an integral part of my research and supportive throughout my graduate studies: A huge thank you to Dr. Norman Ellstrand as my major professor and graduate advisor, and to my supervisor, Dr. Tracy Kahn, who helped influence my decision to go back to graduate school while allowing me to continue my full-time employment with the UC Riverside Citrus Variety Collection. Norm and Tracy, my UCR parents, provided such amazing enthusiasm, guidance and friendship while I was working, going to school and caring for my growing family. Their support was critical and I could not have done this without them. My committee members, Dr. Timothy Close and Dr. Robert Krueger for their valuable advice, feedback and suggestions. Robert Krueger for mentoring me over the past twelve years. He was the first person I met at UCR and his willingness to help expand my knowledge base on Citrus varieties has been a generous gift. He is also an amazing friend. Tim Williams for teaching me everything I know about breeding Citrus and without whom I'd have never discovered my love for the art. -
Tropical Horticulture: Lecture 32 1
Tropical Horticulture: Lecture 32 Lecture 32 Citrus Citrus: Citrus spp., Rutaceae Citrus are subtropical, evergreen plants originating in southeast Asia and the Malay archipelago but the precise origins are obscure. There are about 1600 species in the subfamily Aurantioideae. The tribe Citreae has 13 genera, most of which are graft and cross compatible with the genus Citrus. There are some tropical species (pomelo). All Citrus combined are the most important fruit crop next to grape. 1 Tropical Horticulture: Lecture 32 The common features are a superior ovary on a raised disc, transparent (pellucid) dots on leaves, and the presence of aromatic oils in leaves and fruits. Citrus has increased in importance in the United States with the development of frozen concentrate which is much superior to canned citrus juice. Per-capita consumption in the US is extremely high. Citrus mitis (calamondin), a miniature orange, is widely grown as an ornamental house pot plant. History Citrus is first mentioned in Chinese literature in 2200 BCE. First citrus in Europe seems to have been the citron, a fruit which has religious significance in Jewish festivals. Mentioned in 310 BCE by Theophrastus. Lemons and limes and sour orange may have been mutations of the citron. The Romans grew sour orange and lemons in 50–100 CE; the first mention of sweet orange in Europe was made in 1400. Columbus brought citrus on his second voyage in 1493 and the first plantation started in Haiti. In 1565 the first citrus was brought to the US in Saint Augustine. 2 Tropical Horticulture: Lecture 32 Taxonomy Citrus classification based on morphology of mature fruit (e.g. -
Polyembryony
COMPILED AND CIRCULATED BY DR. PRITHWI GHOSH, ASSISTANT PROFESSOR, DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE Polyembryony [Introduction; Classification; Causes and applications] As per the name Polyembryony – it refers to the development of many embryos. • Occurrence of more than one embryo in a seed is called polyembryony. • When two or more than two embryos develop from a single fertilized egg, then this phenomenon is known as Polyembryony. • In the case of humans, it results in forming two identical twins. This phenomenon is found both in plants and animals. • The best example of Polyembryony in the animal kingdom is the nine-banded armadillo. It is a medium-sized mammal found in certain parts of America and this wild species gives birth to identical quadruplets. Polyembryony in Plants • The production of two or more than two embryos from a single seed or fertilized egg is termed as Polyembryony. • In plants, this phenomenon is caused either due to the fertilization of one or more than one embryonic sac or due to the origination of embryos outside of the embryonic sac. • This natural phenomenon was first discovered in the year 1719 by Antonie van Leeuwenhoek in Citrus plant seeds. Types of Polyembryony BOTANY: SEM-V, PAPER-C11T: REPRODUCTIVE BIOLOGY OF ANGIOSPERMS, UNITS 7: POLYEMBRYONY AND APOMIXIS COMPILED AND CIRCULATED BY DR. PRITHWI GHOSH, ASSISTANT PROFESSOR, DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE There are two different types of polyembryony: 1. Induced Polyembryony – experimentally induced 2. Spontaneous Polyembryony – naturally occurring According to Webber, polyembryony is classified into three different types: 1. Cleavage Polyembryony: In the case of this type, a single fertilized egg gives rise to a number of embryos. -
Comparative Transcriptome Sequencing to Determine Genes Related to the Nucellar Embryony Mechanism in Citrus
Turkish Journal of Agriculture and Forestry Turk J Agric For (2019) 43: 58-68 http://journals.tubitak.gov.tr/agriculture/ © TÜBİTAK Research Article doi:10.3906/tar-1806-10 Comparative transcriptome sequencing to determine genes related to the nucellar embryony mechanism in citrus 1, 2 1 1 1 Özhan ŞİMŞEK *, Dicle DÖNMEZ , Sinan ETİ , Turgut YEŞİLOĞLU , Yıldız AKA KAÇAR 1 Department of Horticulture, Faculty of Agriculture, Çukurova University, Adana, Turkey 2 Biotechnology Research and Application Center, Çukurova University, Adana, Turkey Received: 04.06.2018 Accepted/Published Online: 14.10.2018 Final Version: 06.02.2019 Abstract: Several citrus varieties produce apomictic seeds by the nucellar embryony (NE) mechanism. Nucellar embryos are created from nucellus tissue and have an identical genetic constitution to the mother plant. Nucellar embryony is known to be an unusual feature of seed production in many citrus cultivars. The term “NE” refers to the development of identical embryos from the maternal tissue known as the nucellus surrounding the embryo sac. The authors aimed here to detect differentially expressed genes involved in the NE mechanism. Orlando tangelo (OT), producing apomictic seeds, and a clementine mandarin, Algerian tangerine ranch selection (AT), known as monoembryonic, were used for high-throughput transcriptome sequencing. First of all, histological analysis was used to determine the initial stage of the development of NE cells. Initial NE cells began to develop on the third day after anthesis. Based on the histological analysis, ovules of flower buds for OT were sampled at the balloon stage and 1, 3, and 5 days after anthesis; for AT only ovules of flower buds at the balloon stage and 3 days after anthesis were sampled for comparative transcriptome sequencing. -
Improvement of Subtropical Fruit Crops: Citrus
IMPROVEMENT OF SUBTROPICAL FRUIT CROPS: CITRUS HAMILTON P. ÏRAUB, Senior Iloriiciilturist T. RALPH ROBCNSON, Senior Physiolo- gist Division of Frnil and Vegetable Crops and Diseases, Bureau of Plant Tndusiry MORE than half of the 13 fruit crops known to have been cultivated longer than 4,000 years,according to the researches of DeCandolle (7)\ are tropical and subtropical fruits—mango, oliv^e, fig, date, banana, jujube, and pomegranate. The citrus fruits as a group, the lychee, and the persimmon have been cultivated for thousands of years in the Orient; the avocado and papaya were important food crops in the American Tropics and subtropics long before the discovery of the New World. Other types, such as the pineapple, granadilla, cherimoya, jaboticaba, etc., are of more recent introduction, and some of these have not received the attention of the plant breeder to any appreciable extent. Through the centuries preceding recorded history and up to recent times, progress in the improvement of most subtropical fruits was accomplished by the trial-error method, which is crude and usually expensive if measured by modern standards. With the general accept- ance of the Mendelian principles of heredity—unit characters, domi- nance, and segregation—early in the twentieth century a starting point was provided for the development of a truly modern science of genetics. In this article it is the purpose to consider how subtropical citrus fruit crops have been improved, are now being improved, or are likel3^ to be improved by scientific breeding. Each of the more important crops will be considered more or less in detail. -
Citrus Rootstocks: Their Characters and Reactions
CITRUS ROOTSTOCKS: THEIR CHARACTERS AND REACTIONS (an unpublished manuscript) ca. 1986 By W. P. BITTERS (1915 – 2006) Editor, digital version: Marty Nemeth, Reference Librarian, UC Riverside Science Library, retired Subject matter experts, digital version: Dr. Tracy Kahn, Curator, UC Citrus Variety Collection Dr. Robert Krueger, Curator, USDA-ARS National Clonal Germplasm Repository for Citrus & Dates Toni Siebert, Assistant Curator, UC Citrus Variety Collection ca. 1955 ca. 1970 IN MEMORIUM Willard P. Bitters Professor of Horticulture, Emeritus Riverside 1915-2006 Born in Eau Claire, Wisconsin, in June, 1915, Dr. Willard “Bill” Bitters earned his bachelor’s degree in biology from St. Norbert College and his master’s degree and Ph.D. from the University of Wisconsin. After earning his doctorate, he first worked as the superintendent of the Valley Research Farm of the University of Arizona in Yuma, and joined the Citrus Experiment Station, in Riverside in 1946 as a Horticulturist. In 1961, Dr. Bitters became a Professor in the newly established University of California-Riverside. His initial assignment was to work on horticultural aspects of tristeza, a serious vector-transmitted virus disease which threatened to destroy California citrus orchards. Tristeza was already in California and spreading in 1946. At that time most citrus trees in California were grafted on a rootstock that was known to be susceptible to tristeza. Dr. Bill Bitters was responsible for screening of over 500 cultivars to determine which rootstock-scion combinations were resistant to this disease and yet possessed suitable horticultural characteristics. Of the 500 screened, most were susceptible, but several successful ones were selected and released to the industry. -
Spiteful Soldiers and Sex Ratio Conflict in Polyembryonic
vol. 169, no. 4 the american naturalist april 2007 Spiteful Soldiers and Sex Ratio Conflict in Polyembryonic Parasitoid Wasps Andy Gardner,1,2,* Ian C. W. Hardy,3 Peter D. Taylor,1 and Stuart A. West4 1. Department of Mathematics and Statistics, Queen’s University, Kingston, Ontario K7L 3N6, Canada; Behaviors that reduce the fitness of the actor pose a prob- 2. Department of Biology, Queen’s University, Kingston, Ontario lem for evolutionary theory. Hamilton’s (1963, 1964) in- K7L 3N6, Canada; clusive fitness theory provides an explanation for such 3. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom; behaviors by showing that they can be favored because of 4. Institute of Evolutionary Biology, School of Biological Sciences, their effects on relatives. This is encapsulated by Hamil- University of Edinburgh, King’s Buildings, Edinburgh EH9 3JT, ton’s (1963, 1964) rule, which states that a behavior will United Kingdom be favored when rb 1 c, where c is the fitness cost to the actor, b is the fitness benefit to the recipient, and r is their Submitted September 15, 2006; Accepted November 7, 2006; genetic relatedness. Hamilton’s rule provides an expla- Electronically published February 5, 2007 nation for altruistic behaviors, which give a benefit to the recipient (b 1 0) and a cost to the actor (c 1 0): altruism is favored if relatedness is sufficiently positive (r 1 0) so that rb Ϫ c 1 0. The idea here is that by helping a close abstract: The existence of spiteful behaviors remains controversial. -
Genomic Analyses of Primitive, Wild and Cultivated Citrus Provide Insights Into Asexual Reproduction
ARTICLES OPEN Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction Xia Wang1,7, Yuantao Xu1,7, Siqi Zhang1,7, Li Cao2,7, Yue Huang1, Junfeng Cheng3, Guizhi Wu1, Shilin Tian4, Chunli Chen5, Yan Liu3, Huiwen Yu1, Xiaoming Yang1, Hong Lan1, Nan Wang1, Lun Wang1, Jidi Xu1, Xiaolin Jiang1, Zongzhou Xie1, Meilian Tan1, Robert M Larkin1, Ling-Ling Chen3, Bin-Guang Ma3, Yijun Ruan5,6, Xiuxin Deng1 & Qiang Xu1 The emergence of apomixis—the transition from sexual to asexual reproduction—is a prominent feature of modern citrus. Here we de novo sequenced and comprehensively studied the genomes of four representative citrus species. Additionally, we sequenced 100 accessions of primitive, wild and cultivated citrus. Comparative population analysis suggested that genomic regions harboring energy- and reproduction-associated genes are probably under selection in cultivated citrus. We also narrowed the genetic locus responsible for citrus polyembryony, a form of apomixis, to an 80-kb region containing 11 candidate genes. One of these, CitRWP, is expressed at higher levels in ovules of polyembryonic cultivars. We found a miniature inverted-repeat transposable element insertion in the promoter region of CitRWP that cosegregated with polyembryony. This study provides new insights into citrus apomixis and constitutes a promising resource for the mining of agriculturally important genes. Asexual reproduction is a remarkable feature of perennial fruit crops important trait for breeding purposes. On the one hand, polyembryony that facilitates the faithful propagation of commercially valuable is widely employed in citrus nurseries and propagation programs to individuals by avoiding the uncertainty associated with the sexual generate large numbers of uniform rootstocks from seeds. -
A Pattern of Unique Embryogenesis Occurring Via Apomixis in Carya Cathayensis
BIOLOGIA PLANTARUM 56 (4): 620-627, 2012 DOI: 10.1007/s10535-012-0256-2 A pattern of unique embryogenesis occurring via apomixis in Carya cathayensis B. ZHANG1,2a, Z.J. WANG1a, S.H. JIN1a, G.H. XIA1, Y.J. HUANG1 and J.Q. HUANG1* School of Forestry and Biotechnology, Zhejiang A & F University, Lin’an 311300, P.R. China1 Bureau of Agricultural Economics of Haiyan, Haiyan 314300, P.R. China2 Abstract Apomixis represents an alteration of classical sexual plant reproduction to produce seeds that have essentially clonal embryos. In this report, hickory (Carya cathayensis Sarg.), which is an important oil tree, is identified as a new apomictic species. The ovary has a chamber containing one ovule that is unitegmic and orthotropous. Embryological investigations indicated that the developmental pattern of embryo sac formation is typical polygonum-type. Zygote embryos were not found during numerous histological investigations, and the embryo originated from nucellar cells. Nucellar embryo initials were found both at the micropylar and chalazal ends of the embryo sac, but the mature embryo developed only at the nucellar beak region. The mass of the nucellar embryo initial at the nucellar beak region developed into a nucellar embryo or split into two nucellar proembryos. The later development of the nucellar embryo was similar to the zygotic embryo and progressed from globular embryo to heart-shape embryo and to cotyledon embryo. Additional key words: adventitious embryony, female gametophyte, hickory, nucellar embryo. Introduction The term apomixis was first introduced by Winkler 3) two gametophytic types diplospory and apospory (1908) to refer to the “substitution of sexual reproduction (Peggy 2006, Koltunow 1993). -
Copidosoma Floridanum
Sakamoto et al. BMC Genomics (2020) 21:152 https://doi.org/10.1186/s12864-020-6559-3 RESEARCH ARTICLE Open Access Analysis of molecular mechanism for acceleration of polyembryony using gene functional annotation pipeline in Copidosoma floridanum Takuma Sakamoto1, Maaya Nishiko2, Hidemasa Bono3, Takeru Nakazato3, Jin Yoshimura4,5,6, Hiroko Tabunoki1,2* and Kikuo Iwabuchi2 Abstract Background: Polyembryony is defined as the formation of several embryos from a single egg. This phenomenon can occur in humans, armadillo, and some endoparasitoid insects. However, the mechanism underlying polyembryogenesis in animals remains to be elucidated. The polyembryonic parasitoid wasp Copidosoma floridanum oviposits its egg into an egg of the host insect; eventually, over 2000 individuals will arise from one egg. Previously, we reported that polyembryogenesis is enhanced when the juvenile hormone (JH) added to the culture medium in the embryo culture. Hence, in the present study, we performed RNA sequencing (RNA-Seq) analysis to investigate the molecular mechanisms controlling polyembryogenesis of C. floridanum. Functional annotation of genes is not fully available for C.floridanum; however, whole genome assembly has been archived. Hence, we constructed a pipeline for gene functional annotation in C. floridanum and performed molecular network analysis. We analyzed differentially expressed genes between control and JH-treated molura after 48 h of culture, then used the tblastx program to assign whole C. floridanum transcripts to human gene. Results: We obtained 11,117 transcripts in the JH treatment group and identified 217 differentially expressed genes compared with the control group. As a result, 76% of C. floridanum transcripts were assigned to human genes. -
Embryogenesis, Polyembryony, and Settlement in the Gorgonian Plexaura Homomalla
bioRxiv preprint doi: https://doi.org/10.1101/2020.03.19.999300; this version posted March 20, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Embryogenesis, polyembryony, and settlement in the gorgonian Plexaura homomalla Christopher D. Wells1†*, Kaitlyn J. Tonra1*, and Howard R. Lasker1,2 *Equal contributors, first author decided by a coin flip 1. Department of Geology, University at Buffalo, State University of New York, Buffalo, NY 2. Department of Environment and Sustainability, State University of New York at Buffalo, Buffalo, NY † Email: [email protected] Keywords: cleavage polyembryony, Cnidaria, maternal investment, Octocorallia, Plexauridae ABSTRACT Understanding the ontogeny and reproductive biology of reef-building organisms can shed light on patterns of population biology and community structure. This knowledge is particularly important for Caribbean octocorals, which seem to be more resilient to long-term environmental change than scleractinian corals and provide some of the same ecological services. We monitored the development of the black sea rod Plexaura homomalla, a common, widely distributed octocoral on shallow Caribbean reefs, from eggs to 3-polyp colonies over the course of 73 days. In aquaria on St John, U.S. Virgin Islands, gametes were released in spawning events three to six days after the July full moon. Cleavage started 3 hours after fertilization and was holoblastic, equal, and radial. Embryos were positively buoyant until becoming planulae. Planulae were competent after 4 days.