Synthèse Organique D'apo-Lycopénoïdes, Étude Des

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Synthèse Organique D'apo-Lycopénoïdes, Étude Des Synthèse organique d’apo-lycopénoïdes, étude des propriétés antioxydantes et de complexation avec l’albumine de sérum humain Eric Reynaud To cite this version: Eric Reynaud. Synthèse organique d’apo-lycopénoïdes, étude des propriétés antioxydantes et de com- plexation avec l’albumine de sérum humain. Sciences agricoles. Université d’Avignon, 2009. Français. NNT : 2009AVIG0231. tel-00870922 HAL Id: tel-00870922 https://tel.archives-ouvertes.fr/tel-00870922 Submitted on 8 Oct 2013 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. ACADEMIE D’AIX-MARSEILLE UNIVERSITE D’AVIGNON ET DES PAYS DE VAUCLUSE THESE présentée pour obtenir le grade de Docteur en Sciences de l’Université d’Avignon et des Pays de Vaucluse SPECIALITE : Chimie SYNTHESE ORGANIQUE D'APO-LYCOPENOÏDES ETUDE DES PROPRIETES ANTIOXYDANTES ET DE COMPLEXATION AVEC L'ALBUMINE DE SERUM HUMAIN par Eric REYNAUD soutenue le 23 novembre 2009 devant un jury composé de Hanspeter PFANDER Professeur, Université de Berne (Suisse) Rapporteur Catherine BELLE Chargée de recherche, CNRS Grenoble Rapporteur Paul-Henri DUCROT Directeur de recherche, INRA Versailles Examinateur Patrick BOREL Directeur de recherche, INRA Marseille Examinateur Olivier DANGLES Professeur, Université Avignon Directeur de thèse Catherine CARIS-VEYRAT Chargée de recherche, INRA Avignon Directeur de thèse Ecole doctorale 306 UMR 408, SQPOV A Je remercie l’ensemble du jury pour avoir accepté de juger ce travail : -Pr. Dr. h. c. Hanspeter PFANDER (rapporteur) -Dr. Catherine BELLE (rapporteur) -Dr. Paul-Henri DUCROT (examinateur) -Dr. Patrick BOREL (examinateur) Je remercie Monsieur Christophe N’GUYEN THE pour son accueil dans l’unité mixte de recherche qu’il dirige. Je tiens à remercier très chaleureusement mes deux directeurs de thèse pour m’avoir fait confiance tout au long de ces trois années concernant mon projet de recherche. A Catherine CARIS-VEYRAT pour m’avoir soutenu et encouragé tant au niveau scientifique que humain, pour sa disponibilité, sans oublier sa gaieté communicative. A Olivier DANGLES pour ses conseils enrichissants son optimisme ainsi que pour avoir suivi ce travail avec beaucoup de patience et de rigueur. Je tiens à remercier Michel CARAIL pour son aide quotidienne, son soutien dans les moments difficiles et surtout pour son amitié. Je remercie Pascale GOUPY pour sa grande participation et ses conseils précieux concernant les "manips" de pouvoir antioxydant ainsi que pour son enthousiasme. Je remercie Michelle LOONIS pour son aide concernant les études sur l’albumine de sérum humain. Je remercie tous les membres de l’équipe et de l'unité SQPOV en particulier, Claire DUFOUR, Christian GINIES, Marie-José VALLIER, Gérard BEAUVILLE, Eric PIETRY, Dominique LOONIS, Marielle BAUGE, Line TICHIT, David PAGES, Catherine RENARD, Julien BRILLARD pour leur gentillesse au quotidien. Je ne saurais oublier tous mes collègues de boulot avec qui j’ai passé des moments exceptionnels, Guillaume QUINONERO, Cyril KELLER, Yann LEBLANC, Adeline MOISES, Bénédicte LORRAIN, Aurélie CENDRES, Charlotte SY et Céline CHANFORAN. Je remercie pour leur sympathie tous les membres de l’Université d’Avignon Valérie TOMAO, Nathalie MORA-SOUMILLE, Njara RAKOTOMANOMANA ainsi que mes amis Matthieu VIROT, Stéphanie GALLAND et Guillaume CUOCCO. Je remercie tous les membres du projet européen « LYCOCARD » et plus particulièrement les équipes de Marseille, Jena et Débrécen. Je remercie Bettina WUESTENBERG et Simon WENER de la société DSM Nutritional Product pour leur intérêt en vers notre laboratoire mais aussi pour la gentillesse qu'ils m'ont témoigné durant mon stage dans leurs laboratoires. Je remercie Hansgeorg ERNST de la société BASF pour nous avoir fourni la pseudoionone et le vinylpseudoionol. Un très grand merci à Joëlle FIDANZA de l’Université d’Avignon sans qui je n’aurais jamais pu être ici aujourd’hui. Je remercie toute ma famille de m’avoir soutenu tout au long de mes études. BCDEFCA BCDEFCA ACAFAF AFA CA!FCA!F""" #CEA" $%AAAECA " $%AA" $$ECA & '(A)FCA!F '(A)FE*A(E*(*)*)CA+, $-EC*)(A. -CACAF*)(A" , CA!F/-AC*0(CA12 ,*AFCA!F3 , E3 ,$AAAEFC4(FCA5$ ,$6)0EC*ECA/*CFE4(EA $ ,$$6)0E)/7ECA4(FCA8$$ ,955ACAFCA!F$ ,,:EE(*$. ,,%AA $. 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Ses recherches s'organisent autour de trois pôles de compétences qui sont le pôle horticole intégré, le pôle d'adaptation au changement global et le pôle de santé des plantes. H,<&H,<&????%AAICEAFFAFJ=AC%AAICEAFFAFJ=ACEE Les objectifs de cette unité sont d'améliorer les caractéristiques organoleptiques, hygiéniques et nutritionnelles des fruits et légumes frais et transformés. Les travaux de recherche concernent l’ensemble de la filière - élaboration de la qualité avant récolte, - mise au point de technologies de conservation ou de transformations permettant de valoriser au mieux cette qualité, - intérêt en nutrition préventive et maîtrise du risque microbiologique associé au développement des produits réfrigérés prêt à l’emploi. Pour répondre à ces objectifs, l’activité est répartie entre trois équipes de recherches : - Microbiologie et Sécurité des aliments - Qualité des fruits - Chimie des antioxydants 91*6)FC91*6)FCAAAA4(FCA4(FCA4(FCA L’activité de l’équipe chimie des antioxydants est centrée sur les microconstituants des plantes d’importance alimentaire, en particulier les polyphénols et les caroténoïdes.
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