DOCSLIB.ORG

A New Violet Brown Aureoboletus (Boletaceae) from Guangdong of China

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A New Violet Brown Aureoboletus (Boletaceae) from Guangdong of China mycoscience 56 (2015) 481e485 Available online at www.sciencedirect.com journal homepage: www.elsevier.com/locate/myc Short communication A new violet brown Aureoboletus (Boletaceae) from Guangdong of China * Ming Zhang a,b, Tai-Hui Li a,b, , Jiang Xu a,b, Bin Song b a School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China b State Key Laboratory of Applied Microbiology (Southern China), Guangdong Institute of Microbiology, Guangzhou 510070, China article info abstract Article history: Aureoboletus marroninus sp. nov. is described, illustrated and compared with phenetically Received 6 June 2014 similar and phylogenetically related species. Morphologically, it is characterized by its Received in revised form small basidioma with a pileus of 13e22 mm broad, reddish brown to brownish violet or 11 February 2015 violet brown, glutinous and wrinkled pileus fringed with gelatinized veil remnants at the Accepted 12 February 2015 margin, and basidiospores of (8e)8.5e10  4e4.5(e5) mm in size. Phylogenetic analysis of Available online 18 March 2015 the new species and related taxa based on large subunit of nuclear ribosomal RNA gene (LSU) sequences is provided. Morphological and molecular data show that the new species Keywords: should be placed in genus Aureoboletus, and it is clearly different from any known taxon. Basidiomycota © 2015 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. Boletales Taxonomy The genus Aureoboletus was circumscribed by Pouzar (1957) collection is actually a species of Boletus (Zang et al. 1993; with A. gentilis (Quel.) Pouzar as the type species, renowned Klofac 2010); and the species A. reticuloceps has been trans- for its viscid pileus and attractive bright yellow and un- ferred into the genus Boletus based on morphological and changing hymenophore. It is a small genus in the family molecular evidences (Zang et al. 1993; Wang and Yao 2005; Boletaceae and comprises 14 known species and varieties Dentinger et al. 2010; Feng et al. 2012). Recently a new spe- (Klofac 2010, http://www.Indexfungorum.org). Until now, 5 cies belonging to Aureoboletus was discovered from southern species, i.e. A. gentilis (Quel.) Pouzar, A. reticuloceps M. Zang, China, and its formal description, photographs of basidiomata M.S. Yuan & M.Q. Gong, A. tenuis T.H. Li & M. Zhang, A. thibe- and the phylogenetic analysis on the basis of LSU sequences tanus (Pat.) Hongo & Nagas. (ss. Patouillard 1895, non Hongo are provided in this article. and Nagasawa 1980) and A. zangii X.F. Shi & P.G. Liu, have Photographs of basidiomata were taken in the type locality been reported from China (Patouillard 1895; Zang et al. 1993; when collected. Specimens were dried and deposited in the Ying and Zang 1994; Yang et al. 2003; Zang 2006; Shi and Liu Fungal Herbarium of Guangdong Institute of Microbiology 2013; Zhang et al. 2014b). However, the Chinese record of A. (GDGM). Descriptions of macromorphology and the habitat gentilis should be excluded because the misidentified were based upon field notes and photos. Colors were recorded * Corresponding author. School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China. Tel./ fax: þ86 20 87137619. E-mail address: [email protected] (T.-H. Li). http://dx.doi.org/10.1016/j.myc.2015.02.002 1340-3540/© 2015 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. 482 mycoscience 56 (2015) 481e485 and described in general terms following Kornerup and changing to purplish pink or bluish in places; tubes yellowish Wanscher (1978). Micromorphological features were white initially, later grayish yellow to olive yellow; stipe observed on dried material after sectioning and mounting in glutinous, with white basal mycelium; basidiospores (8e) 5% KOH solution, 1% Congo Red or Melzer's reagent. The no- 8.5e10  4e4.5(e5) mm. Differs from A. thibetanus by having a tation (ae)bec(ed) was used to describe basidiospores di- much smaller, darker (brownish red to violet brown) pileus mensions, where the range bec represents 90% or more of the and shorter basidiospores. measured values and ‘a’ and ‘d’ were the extreme values. Q Type: China, Guangdong Province, Chebaling National referred to the length/width ratio of an individual basidio- Nature Reserve, at 24430N, 114120E, alt. 500 m, 11 July 2013, J. spore; Qm referred to the average Q of all Xu, T. Li, B. Song and W. Wang (holotype, GDGM 43288). basidiospores ± sample standard deviation. Sections were LSU sequence ex-holotype: KJ488958. studied at a magnification of up to  1000 using an Olympus Etymology: The specific epithet marroninus indicates the BX51 microscope. All line-drawings of microstructures were maroon color of the pileus. made from rehydrated material and based on microscopic Basidiomata small (Fig. 1). Pileus 13e22 mm broad, obtuse images. to convex, becoming broadly convex to plane, reddish brown Genomic DNA of the new species was extracted from the (9D8e9E8), brownish red to brownish violet or violet brown herbarium specimen using the Sangon Fungus Genomic DNA (maroon) (10C6e10D8, 11D6e11F6, 11D8e11F8 to 13D6e13F6), Extraction kit (Sangon Biotech, Shanghai, China) according to slightly paler towards margin, distinctly wrinkled and often the manufacturer's instructions. The LSU region was ampli- reticulate irregularly with somewhat darker folds at center, fied with primers LR0R and LR5 (Vilgalys and Hester 1990). PCR strongly glutinous when fresh; margin nearly flat and often was performed in a total volume of 20 ml containing 0.5 ml appendiculate with yellowish white to subhyaline, strongly template DNA, 8.5 ml distilled water, 0.5 ml of each primer, and gelatinized veil remnants. Context 2e3 mm thick at the stipe, 10 ml PCR mix [DreamTaq™ Green PCR Master Mix (2Â), Fer- firm and tough in youth, soft when matured, white on the mentas, Vilnius, Lithuania]. Amplification reactions were whole, purplish red (13A3e13B6, 14A3e14C5) to greyish performed in a Tprofessional standard thermocycler (Bio- magenta (13D6e14D6) beneath the pileipellis, practically un- metra, Gottingen,€ Germany) under the following conditions at changing or slightly changing to purplish pink (14A3) when 95 C for 4 min, then followed by 35 cycles of denaturation at exposed, often slightly changing to blue in places especially in 95 C for 60 s, annealing at 53 C for 60 s, extension at 72 C for the lower portion of the stipe. Tubes 4e5 mm deep, distinctly 80 s and a final extension at 72 C for 8 min. The PCR products depressed around stipe, yellowish white (2A2e4A2) when was electrophoresed on 1% agarose gels with known standard young, becoming pale yellow, greyish yellow, pastel yellow to ® DNA marker, and sequenced was performed on an ABI Prism olive yellow (2A3e4A3, 1B3e2B3, 2A4e3A4, 2C6e3C6) in age, 3730 Genetic Analyzer (PE Applied Biosystems, Foster, CA, often with an olive tint, practically unchanging or slightly USA) at Beijing Genomic Institute (BGI) using the same bruising blue. Pores 0.5e0.8 mm in diam., mostly subangular, primers as in the PCR amplification. The sequence of the ho- slightly radially elongated around stipe at maturity, smaller lotype was submitted to GenBank. Other LSU sequences of near pileus margin, concolourous with tubes. Stipe related taxa were downloaded from GenBank. 13e20  2e4 mm, central, cylindrical or narrowly clavate, Sequences were aligned with additional sequences down- solid, equal to slightly enlarged downwards, pale red, pink to loaded from GenBank using ClustalX (Thompson et al. 1997). purplish pink (11A3e12A3, 13A3, 14A3), without reticulation, Alignment was manually adjusted to allow maximum align- smooth to faintly longitudinally striate, gelatinous or strongly ment and to minimize gaps using BioEdit (Hall 1999), and the viscid when young and wet, with white basal mycelium. Odor aligned sequence data matrix has been deposited in TreeBASE and Taste mild. (ID: 15947). Maximum likelihood (ML) analysis was applied to Basidiospores (8e)8.5e10  4e4.5(e5) mm, Q ¼ (1.9e) e e ¼ ± the LSU dataset. In phylogenetic construction, sequence of 2.0 2.4( 2.5), Qm 2.2 0.1, ellipsoid, smooth, yellow to Suillus luteus (L.) Roussel was used as an outgroup (Li et al. yellowish brown in 5% KOH and yellow brown to dark brown 2011). The tree construction procedure was performed in in Melzer's reagent, thin-walled. Basidia 4-spored, MEGA 5.2 (Tamura et al. 2011). According to the Bayesian in- 20e23  8e10 mm, clavate, yellowish white to hyaline in 5% formation criterion (BIC) score, a Kimura 2-parameter model KOH, yellow to yellowish brown in Melzer's reagent, without of nucleotide substitution with a gamma distributed rate basal clamps; sterigmata 2.5e4 mm long. Pleurocystidia heterogeneity and a proportion of invariant sites (K2þGþI) 35e62  7e11 mm, fusiform, thin-walled, yellowish white to was chosen as the optimal substitution model (Tamura et al. hyaline in 5% KOH. Cheilocystidia infrequent, similar to 2011). Clade robustness was assessed using a bootstrap anal- pleurocystidia. Hymenophoral trama subparallel to nearly ysis with 1000 replicates. Bootstrap values greater than or bilateral, yellow to hyaline in 5% KOH, composed of branching equal to 50% were shown on the ML tree. hyphae 6e8 mm wide. Pipeipellis in an early stage of devel- opment usually an ixotrichodermium, composed of Taxonomy frequently septate, thin-walled hyphae of 4e6 mm in diam. embedded in a gelatinous layer, brownish to yellowish white Aureoboletus marroninus T.H. Li & Ming Zhang, sp. nov. in 5% KOH, yellowish white in Melzer's reagent, terminal cells Figs. 1, 2. 26e37  6e12 mm, cylindrical, clavate or nearly fusoid. Stip- MycoBank no.: MB 809068. ipellis a tangled layer of repent to suberect branching hyphae Pileus small, violet brown, wrinkled, glutinous, with veil 5e7 mm in diam., yellowish white to hyaline in 5% KOH, sub- remnants at margin; flesh white, unchanging to slightly gelatinous; terminal elements 30e50  3e7 mm, long and mycoscience 56 (2015) 481e485 483 Fig.
Load more

Recommended publications
  • Phylogenetic Overview of Aureoboletus (Boletaceae, Boletales), with Descriptions of Six New Species from China
    A peer-reviewed open-access journal MycoKeys 61: 111–145 (2019) The Aureoboletus in China 111 doi: 10.3897/mycokeys.61.47520 REVIEW ARTICLE MycoKeys http://mycokeys.pensoft.net Launched to accelerate biodiversity research Phylogenetic overview of Aureoboletus (Boletaceae, Boletales), with descriptions of six new species from China Ming Zhang1, Tai-Hui Li1, Chao-Qun Wang1, Nian-Kai Zeng2, Wang-Qiu Deng1 1 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China 2 Department of Pharmacy, Hainan Medical University, Haikou 571101, China Corresponding author: Tai-Hui Li ([email protected]) Academic editor: M. P. Martín | Received 23 October 2019 | Accepted 29 November 2019 | Published 17 December 2019 Citation: Zhang M, Li T-H, Wang C-Q, Zeng N-K, Deng W-Q (2019)Phylogenetic overview of Aureoboletus (Boletaceae, Boletales), with descriptions of six new species from China. MycoKeys 61: 111–145. https://doi. org/10.3897/mycokeys.61.47520 Abstract In this study, species relationships of the genus Aureoboletus were studied, based on both morphological characteristics and a four-gene (nrLSU, tef1-a, rpb1 and rpb2) phylogenetic inference. Thirty-five species of the genus have been revealed worldwide, forming eight major clades in the phylogenetic tree, of which twenty-four species have been found in China, including six new species: A. glutinosus, A. griseorufescens, A. raphanaceus, A. sinobadius, A. solus, A. velutipes and a new combination A. miniatoaurantiacus (Bi & Loh) Ming Zhang, N.K. Zeng & T.H. Li proposed here.
    [Show full text]
  • Aureoboletus Moravicus Aureoboletus
    © Francisco Sánchez Iglesias [email protected] Condiciones de uso Aureoboletus moravicus (Vacek) Klofac, Öst. Z. Pilzk. 19: 142 (2010) Boletaceae, Boletales, Agaricomycetidae, Agaricomycetes, Agaricomycotina, Basidiomycota, Fungi =?Xerocomus tumidus Fr. Hymenomyc. Eur.:51 (1874) ≡ Boletus moravicus Vacek, Stud. Bot. Čechoslav.: 36 (1946) ≡ Xerocomus moravicus (Vacek) Herink, Česká Mykol. 18: 193 (1964) = Boletus leonis D.A. Reid, Fungorum Rariorum Icones Coloratae 1: 7 (1966) = Xerocomus leonis (D.A. Reid) Alessio, Boletus Dill. ex L. (Saronno): 314 (1985) Material estudiado: Huelva, Galaroza, Navahermosa, El Talenque, Parque Natural Sierra de Aracena y Picos de Aroche, 29SQC0300, 665 m, en bosque mixto de Pinus pinea, Quercus suber y Castanea sativa, sotobosque con Pteridium aquilinum y Cistus laurifolius, 27-09- 2014, leg. Francisco Sánchez Iglesias, JA-CUSSTA 8060. Descripción macroscópica: Píleo de 60-90 mm, hemiesférico, después convexo. Cutícula lisa, seca, finamente velutinosa, no separable, cuarteada en pe- queñas placas poligonales a partir de la zona central, color pardo rojizo-anaranjado. Himenio formado por tubos amarillos me- dianamente largos, hasta de 10 mm, que se abren en poros pequeños, apretados, suavemente angulosos, del mismo color que los tubos, sin cambio de color a la presión, pardeando un poco al madurar. Estípite cilíndrico, fusiforme, de 60-120 x 10-28 mm, engrosado en zona media, afinándose hacia el extremo, de color ocre amarillento, surcado de suaves costillas fibrillosas longitu- dinales más oscuras, más evidentes en la zona media. Micelio basal amarillento. Carne compacta, dulce, blanquecino amarillen- to, algo rosado bajo la cutícula, anaranjado bajo los tubos y amarillo más intenso en la base del pie. Esporada pardo amarillento.
    [Show full text]
  • Xerocomus S. L. in the Light of the Present State of Knowledge
    CZECH MYCOL. 60(1): 29–62, 2008 Xerocomus s. l. in the light of the present state of knowledge JOSEF ŠUTARA Prosetická 239, 415 01 Teplice, Czech Republic [email protected] Šutara J. (2008): Xerocomus s. l. in the light of the present state of knowledge. – Czech Mycol. 60(1): 29–62. The definition of the generic limits of Xerocomus s. l. and particularly the delimitation of this genus from Boletus is very unclear and controversial. During his study of European species of the Boletaceae, the author has come to the conclusion that Xerocomus in a wide concept is a heterogeneous mixture of several groups of species. These groups are separated from each other by different anatomical and some other characters. Also recent molecular studies show that Xerocomus s. l. is not a monophyletic group. In agreement with these facts, the European species of Xerocomus s. l. whose anatomy was studied by the present author are here classified into the following, more distinctly delimited genera: Xerocomus s. str., Phylloporus, Xerocomellus gen. nov., Hemileccinum gen. nov. and Pseudoboletus. Boletus badius and Boletus moravicus, also often treated as species of Xerocomus, are retained for the present in the genus Boletus. The differences between Xerocomus s. str., Phylloporus, Xerocomellus, Hemileccinum, Pseudoboletus and Boletus (which is related to this group of genera) are discussed in detail. Two new genera, Xerocomellus and Hemileccinum, and necessary new combinations of species names are proposed. Key words: Boletaceae, Xerocomus, Xerocomellus, Hemileccinum, generic taxonomy, anatomy, histology. Šutara J. (2008): Rod Xerocomus s. l. ve světle současného stavu znalostí. – Czech Mycol.
    [Show full text]
  • Boletaceae), First Report of a Red-Pored Bolete
    A peer-reviewed open-access journal MycoKeys 49: 73–97Neoboletus (2019) antillanus sp. nov. (Boletaceae), first report of a red-pored bolete... 73 doi: 10.3897/mycokeys.49.33185 RESEARCH ARTICLE MycoKeys http://mycokeys.pensoft.net Launched to accelerate biodiversity research Neoboletus antillanus sp. nov. (Boletaceae), first report of a red-pored bolete from the Dominican Republic and insights on the genus Neoboletus Matteo Gelardi1, Claudio Angelini2,3, Federica Costanzo1, Francesco Dovana4, Beatriz Ortiz-Santana5, Alfredo Vizzini4 1 Via Angelo Custode 4A, I-00061 Anguillara Sabazia, RM, Italy 2 Via Cappuccini 78/8, I-33170 Pordenone, Italy 3 National Botanical Garden of Santo Domingo, Santo Domingo, Dominican Republic 4 Department of Life Sciences and Systems Biology, University of Turin, Viale P.A. Mattioli 25, I-10125 Torino, Italy 5 US Forest Service, Northern Research Station, Center for Forest Mycology Research, One Gifford Pinchot Drive, Madison, Wisconsin 53726, USA Corresponding author: Alfredo Vizzini ([email protected]) Academic editor: M.P. Martín | Received 18 January 2019 | Accepted 12 March 2019 | Published 29 March 2019 Citation: Gelardi M, Angelini C, Costanzo F, Dovana F, Ortiz-Santana B, Vizzini A (2019) Neoboletus antillanus sp. nov. (Boletaceae), first report of a red-pored bolete from the Dominican Republic and insights on the genus Neoboletus. MycoKeys 49: 73–97. https://doi.org/10.3897/mycokeys.49.33185 Abstract Neoboletus antillanus sp. nov. appears to be the only red-pored bolete known from the Dominican Repub- lic to date. It is reported as a novel species to science based on collections gathered in a neotropical lowland mixed broadleaved woodland.
    [Show full text]
  • Boletus Reticuloceps, a New Combination for Aureoboletus Reticuloceps
    ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Sydowia Jahr/Year: 2005 Band/Volume: 57 Autor(en)/Author(s): Wang Q. B., Yao Y.J. Artikel/Article: Boletus reticuloceps, a new combination for Aureoboletus reticuloceps. 131-136 ©Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.at Boletus reticuloceps, a new combination for Aureoboletus reticuloceps Q. B. Wang & Y. J. Yao Systematic Mycology & Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China Q. B. Wang & Y. J. Yao (2005): Boletus reticuloceps, a new combination for Aureoboletus reticuloceps.- Sydowia 57 (1): 131-136. A new combination, Boletus reticuloceps, originally described as Aureo- boletus reticuloceps from Yunnan, China, is proposed and illustrated. Based on the dry pileus, white hymenophore when young (becoming yellow when mature), olive brown to brown basidiospores, the lack of gelatinised veil remnants on both the pileus and the stipe, and the absence of gold-yellow pigment in tramal hyphae, this species must be considered as a member of the genus Boletus. The ochraceous brown to ochraceous pileus densely covered with brown granular squamules and its conspicuously reticulate stipe distinguish this species from any other taxon within Boletus. The distinction between this species and its related taxa, A. thibe- tanus, B. castanopsidis and B. mottiae, is also discussed. Key words: Basidiomycetes, Boletales, China. Zang et al. (1993) recorded nine boletes from China with two new species, Aureoboletus reticuloceps M. Zang, M. S. Yuan & M. Q. Gong and Boletus nigricans M. Zang, M. S.
    [Show full text]
  • Mycology Praha
    f I VO LUM E 52 I / I [ 1— 1 DECEMBER 1999 M y c o l o g y l CZECH SCIENTIFIC SOCIETY FOR MYCOLOGY PRAHA J\AYCn nI .O §r%u v J -< M ^/\YC/-\ ISSN 0009-°476 n | .O r%o v J -< Vol. 52, No. 1, December 1999 CZECH MYCOLOGY ! formerly Česká mykologie published quarterly by the Czech Scientific Society for Mycology EDITORIAL BOARD Editor-in-Cliief ; ZDENĚK POUZAR (Praha) ; Managing editor JAROSLAV KLÁN (Praha) j VLADIMÍR ANTONÍN (Brno) JIŘÍ KUNERT (Olomouc) ! OLGA FASSATIOVÁ (Praha) LUDMILA MARVANOVÁ (Brno) | ROSTISLAV FELLNER (Praha) PETR PIKÁLEK (Praha) ; ALEŠ LEBEDA (Olomouc) MIRKO SVRČEK (Praha) i Czech Mycology is an international scientific journal publishing papers in all aspects of 1 mycology. Publication in the journal is open to members of the Czech Scientific Society i for Mycology and non-members. | Contributions to: Czech Mycology, National Museum, Department of Mycology, Václavské 1 nám. 68, 115 79 Praha 1, Czech Republic. Phone: 02/24497259 or 96151284 j SUBSCRIPTION. Annual subscription is Kč 350,- (including postage). The annual sub­ scription for abroad is US $86,- or DM 136,- (including postage). The annual member­ ship fee of the Czech Scientific Society for Mycology (Kč 270,- or US $60,- for foreigners) includes the journal without any other additional payment. For subscriptions, address changes, payment and further information please contact The Czech Scientific Society for ! Mycology, P.O.Box 106, 11121 Praha 1, Czech Republic. This journal is indexed or abstracted in: i Biological Abstracts, Abstracts of Mycology, Chemical Abstracts, Excerpta Medica, Bib­ liography of Systematic Mycology, Index of Fungi, Review of Plant Pathology, Veterinary Bulletin, CAB Abstracts, Rewicw of Medical and Veterinary Mycology.
    [Show full text]
  • First Records of Xerocomus Chrysonemus (Boletaceae) in the Czech Republic
    CZECH MYCOLOGY 65(2): 157–169, DECEMBER 20, 2013 (ONLINE VERSION, ISSN 1805-1421) First records of Xerocomus chrysonemus (Boletaceae) in the Czech Republic 1 2 3 VÁCLAV JANDA *, MARTIN KŘÍŽ , JIŘÍ REJSEK 1Ondříčkova 29, CZ-130 00 Praha 3, Czech Republic; [email protected] 2National Museum, Mycological Department, Cirkusová 1740, CZ-193 00 Praha 9, Czech Republic; [email protected] 3Poštovská 88, CZ-289 30 Rožďalovice, Czech Republic; [email protected] *corresponding author Janda V., Kříž M., Rejsek J. (2013): First records of Xerocomus chrysonemus (Boletaceae) in the Czech Republic. – Czech Mycol. 65(2): 157–169. The paper details the first collections of Xerocomus chrysonemus in the Czech Republic. The au- thors present a macro- and microscopic description of this species based on the study of material col- lected at five different localities. Characters distinguishing X. chrysonemus from related species of the genus Xerocomus s. str. (X. ferrugineus, X. subtomentosus, and X. silwoodensis) are discussed. The Latin name X. chrysonemus is a combination of the words ‘chryso’ = golden and ‘nema’ = mycelium, which very accurately describes the characteristic feature of this species, the golden yellow mycelium at the base of stipe. Key words: Xerocomus chrysonemus, Boletaceae, description, ecology, Czech Republic. Janda V., Kříž M., Rejsek J. (2013): První nálezy druhu Xerocomus chrysonemus (Boletaceae) v České republice. – Czech Mycol. 65(2): 157–169. Článek informuje o prvních nálezech druhu Xerocomus chrysonemus v České republice. Autoři článku předkládají makroskopický a mikroskopický popis tohoto druhu založený na studiu sbíraného materiálu z pěti různých lokalit. Jsou diskutovány znaky odlišující X. chrysonemus od příbuzných dru- hů rodu Xerocomus s.
    [Show full text]
  • Morphology and Phylogeny Reveal Two New Records of Boletoid Mushrooms for the Indian Mycobiota
    ISSN (E): 2349 – 1183 ISSN (P): 2349 – 9265 4(1): 62–70, 2017 DOI: 10.22271/tpr.201 7.v4.i1 .009 Research article Morphology and phylogeny reveal two new records of boletoid mushrooms for the Indian mycobiota Dyutiparna Chakraborty1, Kamal C. Semwal2, Sinchan Adhikari3, Sobhan K. Mukherjee3 and Kanad Das1* 1Cryptogamic Unit, Botanical Survey of India, P.O. Botanic Garden, Howrah-711103, India 2Department of Biology, College of Sciences, Eritrea Institute of Technology, Mai Nafhi, Asmara, Eritrea 3 Department of Botany, University of Kalyani, Kalyani-741235, Nadia, West Bengal, India *Corresponding Author: [email protected] [Accepted: 16 February 2017] Abstract: A detailed macro- and micromorphological studies coupled with the LSU-based phylogenetic inference of Aureoboletus nephrosporus, a tubulose member of the family Boletaceae is presented. Similarly, another tubulose bolete, Strobilomyces mirandus which was collected both from Eastern and Western Himalayas of India is also reported here with morphological details and ITS-based phylogeny. Both are the new records for this country. Keywords: Boletales - India - Macrofungi - New records - Phylogeny - Sikkim. [Cite as: Chakraborty D, Semwal KC, Adhikari S, Mukherjee SK & Das K (2017) Morphology and phylogeny reveal two new records of boletoid mushrooms for the Indian mycobiota. Tropical Plant Research 4(1): 62–70] INTRODUCTION The members of the family Boletaceae are mostly ectomycorrhizal in tropical to subalpine regions and thus are well represented in Sikkim of the Eastern Himalaya (Lakhanpal 1996, Das 2009, 2012, 2013, Chakraborty & Das 2015, Das & Chakraborty 2014, Das & Dentinger 2015, Das et al. 2012, 2013, 2014, 2015) because of the abundance of suitable host trees like Abies Mill., Picea Mill., Tsuga Carrière, Lithocarpus Blume, Castanopsis (D.
    [Show full text]
  • <I>Aureoboletus Zangii</I> (<I>Boletaceae
    ISSN (print) 0093-4666 © 2013. Mycotaxon, Ltd. ISSN (online) 2154-8889 MYCOTAXON http://dx.doi.org/10.5248/123.451 Volume 123, pp. 451–456 January–March 2013 Aureoboletus zangii (Boletaceae), a new species from China Xiao-Fei Shi 1, 2& Pei-Gui Liu1* 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany Chinese Academy of Sciences, Kunming 650201, Yunnan, China 2 University of Chinese Academy of Sciences, Beijing 100049, China * Correspondence to: [email protected] Abstract —Aureoboletus zangii sp. nov. is described from central China based on morphological and molecular analysis. This species is found fruiting in association with hardwood trees. It is similar to the European A. gentilis and Asian A. thibetanus but is characterized by the yellowish brown or reddish golden basidiomata, glutinous pileus with subtomentum, and viscid stipe. LSU sequence analysis supports the new species in Aureoboletus (Boletaceae). Photographs, line drawings, and a phylogenetic tree showing relationships with closely allied taxa are provided. Key words — Chinese fungal diversity, Boletales, taxonomy Introduction Two Aureoboletus species have previously been reported from China, A. thibetanus (Pat.) Hongo & Nagas. (Ying & Zang 1994) and A. reticuloceps M. Zang et al. (Zang et al. 1993), although the latter species has been transferred to Boletus (Wang & Yao 2005). Worldwide only 10 Aureoboletus species are recognized (Kirk et al. 2008). Although Pouzar named Aureoboletus with the type A. gentilis (Quél.) Pouzar in 1957 (Corner 1972, Kirk et al. 2008), the genus was not accepted for some time (Smith & Thiers 1971, Corner 1972, Singer 1986, Šutara 2005). After recent molecular analyses by Binder (1999) and Binder & Hibbett (2006) helped resolve the taxonomy of the genus, Klofac (2010) presented a world monograph in which he transferred 13 additional taxa to Aureoboletus and presented a species key (including some critical species in other genera).
    [Show full text]
  • The Genus Aureoboletus, a World-Wide Survey. a Contribution to a Monographic Treatment
    ZOBODAT - www.zobodat.at Zoologisch-Botanische Datenbank/Zoological-Botanical Database Digitale Literatur/Digital Literature Zeitschrift/Journal: Österreichische Zeitschrift für Pilzkunde Jahr/Year: 2010 Band/Volume: 19 Autor(en)/Author(s): Klofac Wolfgang Artikel/Article: The genus Aureoboletus, a world-wide survey. A contribution to a monographic treatment. Die Gattung Aureoboletus, ein weltweiter Überblick. Ein Beitrag zu einer monographischen Bearbeitung. 133-174 ©Österreichische Mykologische Gesellschaft, Austria, download unter www.biologiezentrum.at Östcrr. Z. Pilzk. 19(2010) 133 The genus Aureoboletus, a world-wide survey. A contribution to a mono- graphic treatment Die Gattung Aureoboletus, ein weltweiter Überblick. Ein Beitrag zu einer monographischen Bearbeitung WOLFGANG KLOFAC Mayerhöfen 28 A-3074 Michelbach, Austria Accepted 1. 7. 2010 Key words: Basidiomvcola, Boletales, Boletaceae, Aureoboletus. - Taxonomy, species concept, key, new combinations. - Mycoflora of Asia, America, Europe. Abstract: The problem of different interpretations of the autonomy of the genus Aureoboletus is discussed by means of anatomical and other morphological characters, descriptions and illustrations respectively, as well as findings of molecular studies. An annotated survey of Aureoboletus species hitherto described and a world-wide key to the species are given. Species of other genera but likely to be confused with Aureoboletus are discussed and included in the key. For the following taxa the transfer into Aureoboletus resp. a new combination is proposed: Aureoboletus auriporus var. novoguineensis, A. citriniporus, A. fla\>imarginatus, A. flaviporus, A. moravicus, A. moravicus f. pallescens, A. roxanae. Aureoboletus viridiflavus is described as spec. nova. Zusammenfassung: Die Problematik der verschiedenen Auffassungen zur Selbständigkeit der Gattung Aureoboletus („Goldporröhrlinge") wird an Hand anatomischer sowie anderer morphologischer Merkmale, Beschreibungen bzw.
    [Show full text]
  • Revision of Leccinoid Fungi, with Emphasis on North American Taxa
    MYCOLOGIA 2020, VOL. 112, NO. 1, 197–211 https://doi.org/10.1080/00275514.2019.1685351 Revision of leccinoid fungi, with emphasis on North American taxa, based on molecular and morphological data Michael Kuo a and Beatriz Ortiz-Santana b aThe Herbarium of Michael Kuo, P.O. Box 742, Charleston, Illinois 61920; bCenter for Forest Mycology Research, Northern Research Station, United States Department of Agriculture Forest Service, One Gifford Pinchot Drive, Madison, Wisconsin 53726 ABSTRACT ARTICLE HISTORY The leccinoid fungi are boletes and related sequestrate mushrooms (Boletaceae, Basidiomycota) Received 30 April 2019 that have traditionally been placed in Leccinum, Boletus, Leccinellum, and a handful of other less Accepted 23 October 2019 familiar genera. These mushrooms generally feature scabers or scaber-like dots on the surface of KEYWORDS the stipe, and they are often fairly tall and slender when compared with other boletes. They are Basidiomycota; Boletaceae; ectomycorrhizal fungi and appear to be fairly strictly associated with specific trees or groups of Octaviania; Chamonixia; related trees. In the present study, we investigate the phylogenetic relationships among the Leccinellum; Leccinum; leccinoid fungi and other members of the family Boletaceae using portions of three loci from Rossbeevera; Turmalinea;10 nuc 28S rDNA (28S), translation elongation factor 1-α (TEF1), and the RNA polymerase II second- new taxa largest subunit (RPB2). Two DNA data sets (combined 28S-TEF1 and 28S-TEF1-RPB2), comprising sequences from nearly 270 voucher specimens, were evaluated using two different phylogenetic analyses (maximum likelihood and Bayesian inference). Five major clades were obtained, and leccinoid fungi appeared in four of them.
    [Show full text]
  • Complete References List
    Aanen, D. K. & T. W. Kuyper (1999). Intercompatibility tests in the Hebeloma crustuliniforme complex in northwestern Europe. Mycologia 91: 783-795. Aanen, D. K., T. W. Kuyper, T. Boekhout & R. F. Hoekstra (2000). Phylogenetic relationships in the genus Hebeloma based on ITS1 and 2 sequences, with special emphasis on the Hebeloma crustuliniforme complex. Mycologia 92: 269-281. Aanen, D. K. & T. W. Kuyper (2004). A comparison of the application of a biological and phenetic species concept in the Hebeloma crustuliniforme complex within a phylogenetic framework. Persoonia 18: 285-316. Abbott, S. O. & Currah, R. S. (1997). The Helvellaceae: Systematic revision and occurrence in northern and northwestern North America. Mycotaxon 62: 1-125. Abesha, E., G. Caetano-Anollés & K. Høiland (2003). Population genetics and spatial structure of the fairy ring fungus Marasmius oreades in a Norwegian sand dune ecosystem. Mycologia 95: 1021-1031. Abraham, S. P. & A. R. Loeblich III (1995). Gymnopilus palmicola a lignicolous Basidiomycete, growing on the adventitious roots of the palm sabal palmetto in Texas. Principes 39: 84-88. Abrar, S., S. Swapna & M. Krishnappa (2012). Development and morphology of Lysurus cruciatus--an addition to the Indian mycobiota. Mycotaxon 122: 217-282. Accioly, T., R. H. S. F. Cruz, N. M. Assis, N. K. Ishikawa, K. Hosaka, M. P. Martín & I. G. Baseia (2018). Amazonian bird's nest fungi (Basidiomycota): Current knowledge and novelties on Cyathus species. Mycoscience 59: 331-342. Acharya, K., P. Pradhan, N. Chakraborty, A. K. Dutta, S. Saha, S. Sarkar & S. Giri (2010). Two species of Lysurus Fr.: addition to the macrofungi of West Bengal.
    [Show full text]