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Boletaceae), First Report of a Red-Pored Bolete

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Boletaceae), First Report of a Red-Pored Bolete A peer-reviewed open-access journal MycoKeys 49: 73–97Neoboletus (2019) antillanus sp. nov. (Boletaceae), first report of a red-pored bolete... 73 doi: 10.3897/mycokeys.49.33185 RESEARCH ARTICLE MycoKeys http://mycokeys.pensoft.net Launched to accelerate biodiversity research Neoboletus antillanus sp. nov. (Boletaceae), first report of a red-pored bolete from the Dominican Republic and insights on the genus Neoboletus Matteo Gelardi1, Claudio Angelini2,3, Federica Costanzo1, Francesco Dovana4, Beatriz Ortiz-Santana5, Alfredo Vizzini4 1 Via Angelo Custode 4A, I-00061 Anguillara Sabazia, RM, Italy 2 Via Cappuccini 78/8, I-33170 Pordenone, Italy 3 National Botanical Garden of Santo Domingo, Santo Domingo, Dominican Republic 4 Department of Life Sciences and Systems Biology, University of Turin, Viale P.A. Mattioli 25, I-10125 Torino, Italy 5 US Forest Service, Northern Research Station, Center for Forest Mycology Research, One Gifford Pinchot Drive, Madison, Wisconsin 53726, USA Corresponding author: Alfredo Vizzini ([email protected]) Academic editor: M.P. Martín | Received 18 January 2019 | Accepted 12 March 2019 | Published 29 March 2019 Citation: Gelardi M, Angelini C, Costanzo F, Dovana F, Ortiz-Santana B, Vizzini A (2019) Neoboletus antillanus sp. nov. (Boletaceae), first report of a red-pored bolete from the Dominican Republic and insights on the genus Neoboletus. MycoKeys 49: 73–97. https://doi.org/10.3897/mycokeys.49.33185 Abstract Neoboletus antillanus sp. nov. appears to be the only red-pored bolete known from the Dominican Repub- lic to date. It is reported as a novel species to science based on collections gathered in a neotropical lowland mixed broadleaved woodland. A detailed morphological description, color images of fresh basidiomes in habitat and line drawings of the main anatomical features are provided and relationships with phyloge- netically and phenotypically similar taxa are discussed. Three genomic regions (nrITS, nrLSU/28S and rpb2) have been sequenced in order to reinforce the recognition of the new species and to elucidate its taxonomic affiliation within Neoboletus. Keywords Boletales, molecular phylogeny, Greater Antilles, neotropical boletes, Sutorius, taxonomy Copyright Matteo Gelardi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 74 Matteo Gelardi et al. / MycoKeys 49: 73–97 (2019) Introduction In recent times the intensive use of molecular tools applied to the investigation of the systematics of boletoid mushrooms and related groups (order Boletales) has dra- matically revolutionized traditional classifications based on morphological traits, fa- cilitating the research process and leading to the establishment of a novel scientific approach with unexpected taxonomic implications (Bruns and Palmer 1989, Binder 1999, Binder and Bresinsky 2002, Binder et al. 2005, Binder and Hibbett 2006, Nuhn et al. 2013, Wu et al. 2014). In particular, members of the Boletaceae have undergone an extensive reassessment and several new genera have arisen from large, unwieldy and definitely polyphyletic as- semblages such as Boletus Fr., Xerocomus Quél. and Tylopilus P. Karst, just to name a few (Wu et al. 2016b). Among these genera, Neoboletus Gelardi, Simonini & Vizzini has re- cently been segregated from Boletus s.l. (Vizzini 2014), to include taxa orbiting around the generic type Boletus luridiformis Rostk. that were traditionally assigned to either the polyphyletic Boletus sect. Luridi Fr. emend. Lannoy & Estadès (Lannoy and Estadès 2001), Boletus sect. Erythropodes Galli pro parte (Galli 2007) or Boletus subg. Luridellus sect. Immutabiles and sect. Luridiformes pro parte (nom. inval., art. 39.1) (Watling and Hills 2005). Species included in Neoboletus are characterized by boletoid to rarely seco- tioid habit, tomentose to velvety pileus, yellow-olive tubes, brownish, red to orange or more rarely yellow pores, stipe surface usually finely dotted-punctate, yellowish context, tissues quickly and intensely bluing on handling or exposure, mild taste, olive-brown spore print, ellipsoid-fusiform, smooth basidiospores, trichodermal pileipellis consist- ing of filamentous hyphae, hymenophoral trama of the “Boletus-type”, fertile caulohy- menium, inamyloid hyphae in the stipe trama, gymnocarpic ontogenetic development and ectomycorrhizal (ECM) association with members of the Pinaceae and Fagaceae (Vizzini 2014, Simonini and Vizzini 2015, Bessette et al. 2016, Wu et al. 2016a). The separation of Neoboletus from Boletus s. str. and its establishment at the generic rank is phylogenetically strongly supported (Binder and Hibbett 2006, Mello et al. 2006, Hal- ling et al. 2007, 2015; Desjardin et al. 2009, Li et al. 2011, Zeng et al. 2012, Gelardi et al. 2013, 2015; Nuhn et al. 2013, Trappe et al. 2013, Arora and Frank 2014, Vizzini et al. 2014, Wu et al. 2014, Zhao et al. 2014, 2015; Zhu et al. 2014, Chakraborty et al. 2015, Simonini and Vizzini 2015, Smith et al. 2015, Urban and Klofac 2015, Henkel et al. 2016, Liang et al. 2016, Orihara and Smith 2017), the genus being tentatively placed in the “Pulveroboletus group” (Wu et al. 2014), although its taxonomic place- ment within the Boletaceae still remains uncertain (Nuhn et al. 2013, Wu et al. 2014). In contrast to the well-known bolete heritage of North America, Europe and to a less- er degree East Asia, the diversity of the fleshy pored mushrooms in the neotropical forests of Central America and adjacent regions have received only relatively limited attention (e.g. Dennis 1970, Singer et al. 1983, 1990, 1991, 1992; Gómez and Singer 1984, Singer and Gómez 1984, Halling 1989, 1997; Gómez 1997, Halling et al. 1999, 2004, 2008, 2012a, b; Flores Arzù and Simonini 2000, Franco-Molano et al. 2000, Halling and Muel- ler 2002, 2003, 2005; Mata et al. 2003, Mueller et al. 2006, Halling and Ortiz-Santana Neoboletus antillanus sp. nov. (Boletaceae), first report of a red-pored bolete... 75 2009, Flores Arzù et al. 2012, García-Jiménez 2013, although there are many others). Particularly the Caribbean appear to be little explored from the mycological perspective; information is generally widely dispersed and members of the Boletales (including also lamellate and sequestrate representatives) have only sporadically been reported over the past two centuries (Berkeley and Curtis 1869, Hitchcock 1898, Patouillard 1900 1902; Murrill 1910, 1918, 1921; Baker and Dale 1951, Dennis 1970, Kreisel 1971, Singer and Fiard 1976, Reid 1977, Hosford and Trappe 1980, Alphonse 1981, Pegler 1983, 1987; Miller et al. 2000, Guzmán et al. 2004, Camino Vilaró et al. 2006, Ortiz-Santana 2006, Courtecuisse and Welti 2013, Lécuru and Courtecuisse 2013, Moreau et al. 2013). In the Dominican Republic (Hispaniola), as far as the boletoid fungi are concerned and aside from the recent settlement of the genus Phylloporopsis Angelini et al. based on Phylloporus boletinoides A.H. Smith & Thiers (Farid et al. 2018) and a few other reports of boletes an- notated in general publications (Minter et al. 2001, Lodge et al. 2001), the monographic treatment of Ortiz-Santana et al. (2007) currently remains the sole and as yet most com- prehensive taxonomic account dealing with the Boletaceae and Suillaceae for this country. Neoboletus antillanus is described herein as a new species to science using morpho- logical and three-loci (nrITS, nrLSU/28S and rpb2) molecular data, based on multiple collections from a lowland mixed woodland consisting of a number of different neo- tropical broadleaved trees, in purported ECM association with the widespread, na- tively sand-growing littoral seagrape, Coccoloba uvifera (L.) L. (Polygonaceae), a small woody plant naturally distributed throughout the Caribbean basin (Séne et al. 2015, 2018). This notable species appears to be the first and sole red-pored bolete recorded in the Dominican Republic so far and one of the very few ECM members of the Bole- taceae to be found in local lowland deciduous forested ecosystem. The present paper is one in a series of intended contributions devoted to the study of neotropical Boletales, aiming to provide new insights into the taxonomy, phyloge- netic relationships, plant and substrate associations, ecological importance, conserva- tion and biogeographic patterns of the bolete communities occurring in the Domini- can Republic, with continued biodiversity investigations of underexplored areas. Materials and methods Collection site and sampling Specimens examined were collected in a hilly forest near the cemetery of Sousa, in Puerto Plata Province, Dominican Republic, and are deposited in the Herbarium of Jardín Botánico Nacional of Santo Domingo, Dr. Rafael Ma. Moscoso (JBSD) (acro- nym from Thiers 2019), while “ANGE” and ‘‘MG’’ refer to the personal herbarium of Claudio Angelini and Matteo Gelardi, respectively. Herbarium numbers are cited for all collections from which morphological features were examined. Author citations follow the Index Fungorum, Authors of Fungal Names (www.indexfungorum.org/au- thorsoffungalnames.htm). 76 Matteo Gelardi et al. / MycoKeys 49: 73–97 (2019) Morphological studies Macroscopic descriptions and ecological information, such as habitat notations, time of fruiting and associated plant communities accompanied the detailed field notes of the fresh basidiomata. Color terms in capital letters (e.g. Myrtle Green,
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