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Permeability of Rosmarinic Acid in Prunella Vulgaris and Ursolic Acid in Salvia Officinalis Extracts Across Caco-2 Cell Monolayers Zhiyi Qiang Iowa State University

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Permeability of Rosmarinic Acid in Prunella Vulgaris and Ursolic Acid in Salvia Officinalis Extracts Across Caco-2 Cell Monolayers Zhiyi Qiang Iowa State University NCRPIS Publications and Papers North Central Regional Plant Introduction Station 10-11-2011 Permeability of rosmarinic acid in Prunella vulgaris and ursolic acid in Salvia officinalis extracts across Caco-2 cell monolayers Zhiyi Qiang Iowa State University Zhong Ye University of California, Davis Catherine C. Hauck Iowa State University, [email protected] Patricia A. Murphy Iowa State University, [email protected] Joe-Ann McCoy UFonitlloedw St thiatess D aepndar atmddenitt ofion Agalric wulorktures at: http://lib.dr.iastate.edu/ncrpis_pubs Part of the Agricultural Science Commons, Agriculture Commons, Agronomy and Crop See next page for additional authors Sciences Commons, Food Science Commons, and the Horticulture Commons The ompc lete bibliographic information for this item can be found at http://lib.dr.iastate.edu/ ncrpis_pubs/16. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Article is brought to you for free and open access by the North Central Regional Plant Introduction Station at Iowa State University Digital Repository. It has been accepted for inclusion in NCRPIS Publications and Papers by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Permeability of rosmarinic acid in Prunella vulgaris and ursolic acid in Salvia officinalis extracts across Caco-2 cell monolayers Abstract Ethnopharmacological relevance Rosmarinic acid (RA), a caffeic ca id-related compound found in high concentrations in Prunella vulgaris (self- heal), and ursolic acid (UA), a pentacyclic triterpene acid concentrated in Salvia officinalis (sage), have been traditionally used to treat inflammation in the mouth, and may also be beneficial for gastrointestinal health in general. Aim of the study To investigate the permeabilities of RA nda UA as pure compounds and in Prunella vulgaris and Salvia officinalis ethanol extracts across human intestinal epithelial Caco-2 cell monolayers. Materials and methods The permeabilities and phase II biotransformation of RA nda UA as pure compounds and in herbal extracts were compared using Caco-2 cells with HPLC detection. Results −6 The ppa arent permeability coefficient (Papp) for RA nda RA in Prunella vulgaris extracts was 0.2 ± 0.05 × 10 −6 cm/s, significantly increased to 0.9 ± 0.2 × 10 cm/s after β-glucuronidase/sulfatase treatment. Papp for UA and UA in Salvia officinalis extract was 2.7 ± 0.3 × 10−6 cm/s and 2.3 ± 0.5 × 10−6 cm/s before and after β- glucuronidase/sulfatase treatment, respectively. Neither compound was affected in permeability by the herbal extract matrix. Conclusion RA nda UA in herbal extracts had similar uptake as that found using the pure compounds, which may simplify the prediction of compound efficacy, but the apparent lack of intestinal glucuronidation/sulfation of UA is likely to further enhance the bioavailability of that compound compared with RA. Keywords Prunella vulgaris, Salvia officinalis, Rosmarinic acid, Ursolic acid, Permeability, Caco-2, Food Science and Human Nutrition Disciplines Agricultural Science | Agriculture | Agronomy and Crop Sciences | Food Science | Horticulture | Plant Sciences Comments This article is from Journal of Ethnopharmacology 137, no. 3 (11 October 2011): 1107–1112, doi: 10.1016/ j.jep.2011.07.037. This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/ncrpis_pubs/16 Rights Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The onc tent of this document is not copyrighted. Authors Zhiyi Qiang, Zhong Ye, Catherine C. Hauck, Patricia A. Murphy, Joe-Ann McCoy, Mark P. Widrlechner, Manju B. Reddy, and Suzanne Hendrich This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/ncrpis_pubs/16 Journal of Ethnopharmacology 137 (2011) 1107–1112 Contents lists available at ScienceDirect Journal of Ethnopharmacology journa l homepage: www.elsevier.com/locate/jethpharm Permeability of rosmarinic acid in Prunella vulgaris and ursolic acid in Salvia officinalis extracts across Caco-2 cell monolayers a b a a c d,e,f Zhiyi Qiang , Zhong Ye , Cathy Hauck , Patricia A. Murphy , Joe-Ann McCoy , Mark P. Widrlechner , a a,∗ Manju B. Reddy , Suzanne Hendrich a Department of Food Science and Human Nutrition, Iowa State University, Ames, IA, USA b Department of Nutrition, University of California, Davis, CA, USA c Bent Creek Institute, The North Carolina Arboretum, Asheville, NC, USA d US Department of Agriculture-Agricultural Research Service, North Central Regional Plant Introduction Station, Ames, IA, USA e Department of Horticulture, Iowa State University, Ames, IA, USA f Department of Agronomy, Iowa State University, Ames, IA, USA a r t i c l e i n f o a b s t r a c t Article history: Ethnopharmacological relevance: Rosmarinic acid (RA), a caffeic acid-related compound found in high Received 14 March 2011 concentrations in Prunella vulgaris (self-heal), and ursolic acid (UA), a pentacyclic triterpene acid con- Received in revised form 6 July 2011 centrated in Salvia officinalis (sage), have been traditionally used to treat inflammation in the mouth, and Accepted 10 July 2011 may also be beneficial for gastrointestinal health in general. Available online 20 July 2011 Aim of the study: To investigate the permeabilities of RA and UA as pure compounds and in Prunella vulgaris and Salvia officinalis ethanol extracts across human intestinal epithelial Caco-2 cell monolayers. Keywords: Materials and methods: The permeabilities and phase II biotransformation of RA and UA as pure com- Prunella vulgaris pounds and in herbal extracts were compared using Caco-2 cells with HPLC detection. Salvia officinalis Results: The apparent permeability coefficient (Papp) for RA and RA in Prunella vulgaris extracts was Rosmarinic acid −6 −6 ± × ± × ␤ Ursolic acid 0.2 0.05 10 cm/s, significantly increased to 0.9 0.2 10 cm/s after -glucuronidase/sulfatase −6 Permeability treatment. Papp for UA and UA in Salvia officinalis extract was 2.7 ± 0.3 × 10 cm/s and −6 Caco-2 2.3 ± 0.5 × 10 cm/s before and after ␤-glucuronidase/sulfatase treatment, respectively. Neither compound was affected in permeability by the herbal extract matrix. Conclusion: RA and UA in herbal extracts had similar uptake as that found using the pure compounds, which may simplify the prediction of compound efficacy, but the apparent lack of intestinal glucuronida- tion/sulfation of UA is likely to further enhance the bioavailability of that compound compared with RA. © 2011 Elsevier Ireland Ltd. All rights reserved. 1. Introduction but especially concentrated in sage leaves (Salvia officinalis), and inhibits inflammation-related changes in human gingival cells Rosmarinic acid (RA, Fig. 1) is a caffeic acid (CA) derivative (Zdarilová et al., 2009) and in other models (Liu, 1995). This com- found in various botanicals, especially in Prunella vulgaris, a peren- pound also has anti-mutagenic activity (Young et al., 1994). Both nial herb known as self-heal used to treat sore throat, fever, herbs have been used traditionally to treat inflammation in the and wounds (Psotová et al., 2003). RA and Prunella vulgaris limit mouth, and are of interest in inhibiting gastrointestinal inflamma- liver damage derived from a model of bacterial inflammation tion which is relevant to colitis and colon cancer. (Osakabe et al., 2002) and inhibit nervous system inflammation A major limiting step in the utilization of compounds from in another model (Swarup et al., 2007). Ursolic acid (UA, Fig. 1), the diet such as RA and UA is their intestinal absorption and a pentacyclic triterpene acid, is also found in Prunella vulgaris metabolism. Both compounds contain hydroxyls and are likely to be glucuronidated or sulfated in intestinal cells, forms that are generally considered to be less bioactive than parent compounds. Glucuronidation/sulfation has been demonstrated for RA but not for ∗ Corresponding author. Tel.: +1 515 294 4272; fax: +1 515 294 6193. UA, and the plant matrix components might alter this metabolism E-mail addresses: [email protected] (Z. Qiang), [email protected] but this has not been studied yet. The absorption or metabolism (Z. Ye), [email protected] (C. Hauck), [email protected] (P.A. Murphy), of RA has been examined in vivo to a limited extent (Baba et al., [email protected] (J.-A. McCoy), [email protected] 2004, 2005; Konishi et al., 2005). When Perilla extract contain- (M.P. Widrlechner), [email protected] (M.B. Reddy), [email protected] (S. Hendrich). ing 200 mg of RA was orally administered to six men, RA in both 0378-8741/$ – see front matter © 2011 Elsevier Ireland Ltd. All rights reserved. doi:10.1016/j.jep.2011.07.037 1108 Z. Qiang et al. / Journal of Ethnopharmacology 137 (2011) 1107–1112 tory sample: Ames 27664, 27665 and 27748. SD 04ncao01). The resulting seedlings, segregated by accession, were transferred to ◦ flats in a greenhouse (held at 20–25 C). Upper flowering por- tions of 14-month-old plants were harvested at the time of peak flowering, dried, and ground. Four grams of aliquots of the ground samples were extracted with 500 mL of 95% ethanol by Soxhlet percolation for 6 h, filtered, dried by rotary evaporation and lyophilized. Then the extracts were redissolved in 0.5 mL of ◦ ethanol and stored at −20 C under nitrogen. Information about the Prunella vulgaris accessions used for these experiments is avail- Fig. 1. The chemical structures of rosmarinic and ursolic acids. able via the Germplasm Resources Information Network database at http://www.ars-grin.gov/npgs/acc/acc queries.html. plasma and urine was present predominantly as glucuronide and/or sulfate conjugated forms, at 0.6 ± 0.2% and 1.5 ± 0.4% of the total 2.2. HPLC analysis intake, respectively, within 48 h after ingestion (Baba et al., 2005).
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