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Macadamia Second Generation Breeding and Conservation

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Macadamia Second Generation Breeding and Conservation Final Report Macadamia Second Generation Breeding and Conservation Project leader: Dr Bruce Topp Delivery partner: Queensland Alliance for Agriculture and Food Innovation Project code: MC14000 Hort Innovation – Final Report Project: Macadamia Second Generation Breeding and Conservation MC14000 Disclaimer: Horticulture Innovation Australia Limited (Hort Innovation) makes no representations and expressly disclaims all warranties (to the extent permitted by law) about the accuracy, completeness, or currency of information in this Final Report. Users of this Final Report should take independent action to confirm any information in this Final Report before relying on that information in any way. Reliance on any information provided by Hort Innovation is entirely at your own risk. Hort Innovation is not responsible for, and will not be liable for, any loss, damage, claim, expense, cost (including legal costs) or other liability arising in any way (including from Hort Innovation or any other person’s negligence or otherwise) from your use or non-use of the Final Report or from reliance on information contained in the Final Report or that Hort Innovation provides to you by any other means. Funding statement: This project has been funded by Hort Innovation, using the Macadamia research and development levy and contributions from the Australian Government. Hort Innovation is the grower-owned, not-for-profit research and development corporation for Australian horticulture. Publishing details: ISBN 978 0 7341 4595 6 Published and distributed by: Hort Innovation Level 7 141 Walker Street North Sydney NSW 2060 Telephone: (02) 8295 2300 www.horticulture.com.au © Copyright 2019 Horticulture Innovation Australia Limited Hort Innovation – Final Report: MC14000 Macadamia Second Generation Breeding and Conservation Content Macadamia Second Generation Breeding and Conservation ..................................................................... 1 Content ................................................................................................................................................ 3 Summary ............................................................................................................................................. 6 Keywords - 5-10 words ......................................................................................................................... 7 List of abbreviations .............................................................................................................................. 8 Introduction ......................................................................................................................................... 9 Methodology ........................................................................................................................................ 10 1. Second generation of progeny...................................................................................................... 10 1.1 Populations produced by hand pollination (see Appendix 1) ......................................................... 10 1.2 Large populations at reduced cost (see Appendix 1.2) ................................................................. 10 1.3 RVT3 diallel pollination experiment (see Appendix 1.3) ................................................................ 11 1.4 DNA technology to reduce breeding cycle time (see Appendix 1.4) .................................................. 11 2. Rootstocks and reduced tree size (see Appendices 2.1 and 2.2) ........................................................ 12 3. Economic analysis and extension to growers (see Appendix 3) ......................................................... 12 4. Pathology and entomology breeding research (see Appendix 4) ........................................................ 13 5. Wild germplasm trials and development (see Appendix 5) ................................................................ 13 Outputs................................................................................................................................................ 14 1. Second generation of progeny...................................................................................................... 14 1.1 Populations produced by hand pollination (see Appendix 1) ......................................................... 14 1.2 Large populations at reduced cost (see Appendix 1.2) ................................................................. 14 1.3 RVT3 diallel pollination experiment (see Appendix 1.3) ................................................................... 15 1.4 DNA technology to reduce breeding cycle time (see Appendix 1.4) .................................................. 15 2. Rootstocks and reduced tree size (see Appendices 2.1 and 2.2) ........................................................ 15 3. Economic analysis and extension to growers (see Appendix 3) ......................................................... 16 4. Pathology and entomology breeding research (see Appendix 4) ........................................................ 17 5. Germplasm trials and development (see Appendix 5) ....................................................................... 17 Outcomes ............................................................................................................................................ 18 1. Second generation of progeny ........................................................................................................ 18 1.1 Populations produced by hand pollination ...................................................................................... 18 1.2 Large populations at reduced cost ................................................................................................. 18 1.3 RVT3 diallel pollination experiment ................................................................................................ 18 1.4 DNA technology to reduce breeding cycle time .............................................................................. 18 3 Hort Innovation – Final Report: MC14000 Macadamia Second Generation Breeding and Conservation 2. Rootstocks and reduced tree size ................................................................................................. 18 3. Economic analysis and extension to growers (see Appendix 3) ....................................................... 18 4. Pathology and entomology breeding research (see Appendix 4) ..................................................... 19 5. Germplasm trials and development (see Appendix 5) ....................................................................... 19 Monitoring and evaluation ..................................................................................................................... 20 Meetings and workshops .................................................................................................................... 20 Recommendations ................................................................................................................................ 22 Refereed scientific publications .............................................................................................................. 23 Scientific journal articles ..................................................................................................................... 23 Industry publications .......................................................................................................................... 24 Conferences ...................................................................................................................................... 25 Intellectual property, commercialisation and confidentiality ..................................................................... 28 Acknowledgements ............................................................................................................................... 29 Appendices .......................................................................................................................................... 30 Appendix 1.1.1 List of parents for B2.1 progeny population ..................................................................... 30 Appendix 1.1.2 List of families for B2.1 progeny population ..................................................................... 32 Appendix 1.1.3: Selecting breeding progeny from BQBRS12 and BQBRS 13 trials based on a selection index ................................................................................................................................................... 37 Appendix 1.1.3.1: Descriptions of elite selections from B2.1 progeny populations planted in BQBRS12 and BQBRS13 trials ..................................................................................................................................... 44 Appendix 1.3.2: Variability of initial and final nut setting in macadamia superior selections through different pollination methods. ................................................................................................................ 50 Appendix 1.4: Summary of chapters from Katie O’Connor’s PhD thesis: Selection strategies to improve yield in macadamia using component traits and genomics ....................................................................... 51 Appendix 1.4.1: Population structure, genetic diversity and linkage disequilibrium in a macadamia breeding population using SNP and silicoDArT
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