DOCSLIB.ORG

KAT7 (MYST2) Sirna Set I Sirna Duplexes Targeted Against Three Exon Regions

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
KAT7 (MYST2) Sirna Set I Sirna Duplexes Targeted Against Three Exon Regions Catalog # Aliquot Size K316-911-05 3 x 5 nmol K316-911-20 3 x 20 nmol K316-911-50 3 x 50 nmol KAT7 (MYST2) siRNA Set I siRNA duplexes targeted against three exon regions Catalog # K316-911 Lot # Z2049-44 Specificity Formulation KAT7 (MYST2) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human KAT7 (MYST2) expression. shipped at room temperature. Product Description Reconstitution Protocol KAT7 (MYST2) siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human KAT7 collect lyophilized siRNA at the bottom of the tube. (MYST2) mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The gene accession number is NM_007067. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot KAT7; MYST2; HBO1; HBOA 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or KAT7 (MYST2), Active K316-380G below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background KAT7 or K (lysine) acetyltransferase 5 is a signaling protein KAT7 (MYST2) siRNA Set I that belongs to the MYST family of histone acetyl siRNA duplexes targeted against three exon regions transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein which play important roles in Catalog Number K316-911 regulating chromatin remodeling, transcription and other Specific Lot Number Z2049-44 nuclear processes by acetylating histone and nonhistone Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol proteins. KAT7 acts as a transcriptional inhibitor and Format Lyophilized powder inhibited AR-mediated transactivation of reporter Stability 1yr at -70oC from date of shipment constructs in CV-1 and PC-3 cells (1). KAT7 is part of a Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is multisubunit complex that can acetylate histones H3 and recommended that the lyophilized H4 (2). and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can References undergo up to 50 freeze-thaw cycles 1. Sharma, M. et.al: Androgen receptor interacts with a without significant degradation. For long-term storage, it is recommended novel MYST protein, HBO1. J. Biol. Chem. 275: 35200- that the siRNA is stored at -70oC. For 35208, 2000. most favorable performance, avoid 2. Iizuka, M. et.al: Histone acetyltransferase HBO1 repeated handling and multiple freeze/thaw cycles. interacts with the ORC1 subunit of the human initiator protein. J. Biol. Chem. 274: 23027-23034, 1999. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .
Load more

Recommended publications
  • Functional Roles of Bromodomain Proteins in Cancer
    cancers Review Functional Roles of Bromodomain Proteins in Cancer Samuel P. Boyson 1,2, Cong Gao 3, Kathleen Quinn 2,3, Joseph Boyd 3, Hana Paculova 3 , Seth Frietze 3,4,* and Karen C. Glass 1,2,4,* 1 Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, Colchester, VT 05446, USA; [email protected] 2 Department of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USA; [email protected] 3 Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT 05405, USA; [email protected] (C.G.); [email protected] (J.B.); [email protected] (H.P.) 4 University of Vermont Cancer Center, Burlington, VT 05405, USA * Correspondence: [email protected] (S.F.); [email protected] (K.C.G.) Simple Summary: This review provides an in depth analysis of the role of bromodomain-containing proteins in cancer development. As readers of acetylated lysine on nucleosomal histones, bromod- omain proteins are poised to activate gene expression, and often promote cancer progression. We examined changes in gene expression patterns that are observed in bromodomain-containing proteins and associated with specific cancer types. We also mapped the protein–protein interaction network for the human bromodomain-containing proteins, discuss the cellular roles of these epigenetic regu- lators as part of nine different functional groups, and identify bromodomain-specific mechanisms in cancer development. Lastly, we summarize emerging strategies to target bromodomain proteins in cancer therapy, including those that may be essential for overcoming resistance. Overall, this review provides a timely discussion of the different mechanisms of bromodomain-containing pro- Citation: Boyson, S.P.; Gao, C.; teins in cancer, and an updated assessment of their utility as a therapeutic target for a variety of Quinn, K.; Boyd, J.; Paculova, H.; cancer subtypes.
    [Show full text]
  • Overexpression of Androgen Receptor in Prostate Cancer
    ALFONSO URBANUCCI Overexpression of Androgen Receptor in Prostate Cancer ACADEMIC DISSERTATION To be presented, with the permission of the board of Institute of Biomedical Technology of the University of Tampere, for public discussion in the Jarmo Visakorpi Auditorium, of the Arvo Building, Lääkärinkatu 1, Tampere, on January 20th, 2012, at 12 o’clock. UNIVERSITY OF TAMPERE ACADEMIC DISSERTATION University of Tampere, Institute of Biomedical Technology and BioMediTech Tampere University Hospital, Laboratory Centre Graduate Program in Biomedicine and Biotechnology (TGPBB) Finland Supervised by Reviewed by Professor Tapio Visakorpi Docent Auli Karhu University of Tampere University of Helsinki Finland Finland Docent Noora Kotaja University of Turku Finland Copyright ©2012 Tampere University Press and the author Distribution Tel. +358 40 190 9800 Bookshop TAJU Fax +358 3 3551 7685 P.O. Box 617 [email protected] 33014 University of Tampere www.uta.fi/taju Finland http://granum.uta.fi Cover design by Mikko Reinikka Acta Universitatis Tamperensis 1693 Acta Electronica Universitatis Tamperensis 1159 ISBN 978-951-44-8685-2 (print) ISBN 978-951-44-8686-9 (pdf) ISSN-L 1455-1616 ISSN 1456-954X ISSN 1455-1616 http://acta.uta.fi Tampereen Yliopistopaino Oy – Juvenes Print Tampere 2012 CONTENTS ABBREVIATIONS ..................................................................................................... 5 ABSTRACT ................................................................................................................ 7 SINTESI .....................................................................................................................
    [Show full text]
  • Androgen Receptor Interacting Proteins and Coregulators Table
    ANDROGEN RECEPTOR INTERACTING PROTEINS AND COREGULATORS TABLE Compiled by: Lenore K. Beitel, Ph.D. Lady Davis Institute for Medical Research 3755 Cote Ste Catherine Rd, Montreal, Quebec H3T 1E2 Canada Telephone: 514-340-8260 Fax: 514-340-7502 E-Mail: [email protected] Internet: http://androgendb.mcgill.ca Date of this version: 2010-08-03 (includes articles published as of 2009-12-31) Table Legend: Gene: Official symbol with hyperlink to NCBI Entrez Gene entry Protein: Protein name Preferred Name: NCBI Entrez Gene preferred name and alternate names Function: General protein function, categorized as in Heemers HV and Tindall DJ. Endocrine Reviews 28: 778-808, 2007. Coregulator: CoA, coactivator; coR, corepressor; -, not reported/no effect Interactn: Type of interaction. Direct, interacts directly with androgen receptor (AR); indirect, indirect interaction; -, not reported Domain: Interacts with specified AR domain. FL-AR, full-length AR; NTD, N-terminal domain; DBD, DNA-binding domain; h, hinge; LBD, ligand-binding domain; C-term, C-terminal; -, not reported References: Selected references with hyperlink to PubMed abstract. Note: Due to space limitations, all references for each AR-interacting protein/coregulator could not be cited. The reader is advised to consult PubMed for additional references. Also known as: Alternate gene names Gene Protein Preferred Name Function Coregulator Interactn Domain References Also known as AATF AATF/Che-1 apoptosis cell cycle coA direct FL-AR Leister P et al. Signal Transduction 3:17-25, 2003 DED; CHE1; antagonizing regulator Burgdorf S et al. J Biol Chem 279:17524-17534, 2004 CHE-1; AATF transcription factor ACTB actin, beta actin, cytoplasmic 1; cytoskeletal coA - - Ting HJ et al.
    [Show full text]
  • Rabbit Anti-Phospho-MCM2-SL18262R-FITC
    SunLong Biotech Co.,LTD Tel: 0086-571- 56623320 Fax:0086-571- 56623318 E-mail:[email protected] www.sunlongbiotech.com Rabbit Anti-phospho-MCM2 SL18262R-FITC Product Name: Anti-phospho-MCM2 (Ser27)/FITC Chinese Name: FITC标记的磷酸化MCM2蛋白抗体 MCM4 (phospho S27); MCM2(phospho-Ser27); MCM2(phospho Ser27); MCM2 (phospho S27); p-MCM2(Ser27); p-MCM2(S27); MCM2 (phospho S27); p-MCM2 (phospho S27); BM28; CCNL 1; CCNL1; CDC like 1; CDC like-1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; cyclin like-1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome Alias: maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MCM2_MOUSE; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050. Organism Species: Rabbit Clonality: Polyclonalwww.sunlongbiotech.com React Species: Human,Mouse,Rat,Dog,Pig,Rabbit, ICC=1:50-200IF=1:50-200 Applications: not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Molecular weight: 101kDa Form: Lyophilized or Liquid Concentration: 1mg/ml immunogen: phosphopeptide derived from human MCM2 around the phosphorylation site of Ser27 Lsotype: IgG Purification: affinity purified by Protein A Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.
    [Show full text]
  • Anti-Geminin Antibody (Clone 1A8) Mouse Anti Human Monoclonal Antibody Catalog # ALS17716
    10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Anti-Geminin Antibody (clone 1A8) Mouse Anti Human Monoclonal Antibody Catalog # ALS17716 Specification Anti-Geminin Antibody (clone 1A8) - Product Information Application WB, IHC-P, IF, E Primary Accession O75496 Predicted Human Host Mouse Clonality Monoclonal Isotype IgG1,k Calculated MW 23565 Anti-Geminin Antibody (clone 1A8) - Additional Information Gene ID 51053 Alias Symbol GMNN Other Names GMNN, Geminin Target/Specificity Human Geminin Reconstitution & Storage Protein A purified Precautions Anti-Geminin Antibody (clone 1A8) is for research use only and not for use in diagnostic or therapeutic procedures. Anti-Geminin Antibody (clone 1A8) - Protein Information Name GMNN Function Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC) (PubMed:<a href="http://www.uniprot.org/c itations/9635433" target="_blank">9635433</a>, PubMed:<a href="http://www.uniprot.org/ci tations/14993212" target="_blank">14993212</a>, Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 PubMed:<a href="http://www.uniprot.org/ci tations/20129055" target="_blank">20129055</a>, PubMed:<a href="http://www.uniprot.org/ci tations/24064211" target="_blank">24064211</a>). It is degraded during the mitotic phase of the cell cycle (PubMed:<a href="http://www.uni prot.org/citations/9635433" target="_blank">9635433</a>, PubMed:<a href="http://www.uniprot.org/ci tations/14993212" target="_blank">14993212</a>, PubMed:<a href="http://www.uniprot.org/ci tations/24064211" target="_blank">24064211</a>).
    [Show full text]
  • Anti-KAT7 / HBO1 / MYST2 Antibody (ARG66655)
    Product datasheet [email protected] ARG66655 Package: 100 μg anti-KAT7 / HBO1 / MYST2 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes KAT7 / HBO1 / MYST2 Tested Reactivity Hu, Ms Tested Application ChIP, ICC/IF, IP, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name KAT7 / HBO1 / MYST2 Antigen Species Human Immunogen Synthetic peptide within aa. 100-180 of Human KAT7 / HBO1 / MYST2. Conjugation Un-conjugated Alternate Names EC 2.3.1.48; ZC2HC7; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; HBOA; Lysine acetyltransferase 7; Histone acetyltransferase KAT7; MYST-2; HBO1; MYST2; Histone acetyltransferase binding to ORC1 Application Instructions Application table Application Dilution ChIP Assay-dependent ICC/IF 1:200 - 1:1000 IP Assay-dependent WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 71 kDa Observed Size ~ 75 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol and 0.5% BSA Concentration 1 mg/ml www.arigobio.com 1/2 Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use.
    [Show full text]
  • Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia
    Published OnlineFirst May 13, 2019; DOI: 10.1158/2159-8290.CD-18-1474 RESEARCH ARTICLE Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia Emmalee R. Adelman1,2,3, Hsuan-Ting Huang1,2, Alejandro Roisman1,2, André Olsson4, Antonio Colaprico1,2, Tingting Qin5, R. Coleman Lindsley6, Rafael Bejar7, Nathan Salomonis8, H. Leighton Grimes4, and Maria E. Figueroa1,2 Downloaded from cancerdiscovery.aacrjournals.org on October 5, 2021. © 2019 American Association for Cancer Research. Published OnlineFirst May 13, 2019; DOI: 10.1158/2159-8290.CD-18-1474 ABSTRACT Aging is associated with functional decline of hematopoietic stem cells (HSC) as well as an increased risk of myeloid malignancies. We performed an integrative characterization of epigenomic and transcriptomic changes, including single-cell RNA sequencing, dur- ing normal human aging. Lineage−CD34+CD38− cells [HSC-enriched (HSCe)] undergo age-associated epigenetic reprogramming consisting of redistribution of DNA methylation and reductions in H3K27ac, H3K4me1, and H3K4me3. This reprogramming of aged HSCe globally targets developmental and can- cer pathways that are comparably altered in acute myeloid leukemia (AML) of all ages, encompassing loss of 4,646 active enhancers, 3,091 bivalent promoters, and deregulation of several epigenetic modi- fiers and key hematopoietic transcription factors, such as KLF6, BCL6, and RUNX3. Notably,in vitro downregulation of KLF6 results in impaired differentiation, increased colony-forming potential, and changes in expression that recapitulate aging and leukemia signatures. Thus, age-associated epigenetic reprogramming may form a predisposing condition for the development of age-related AML. SIGNIFICANCE: AML, which is more frequent in the elderly, is characterized by epigenetic deregulation.
    [Show full text]
  • A Novel Long Non-Coding RNA-KAT7 Is Low Expressed in Colorectal Cancer
    Wang et al. Cancer Cell Int (2019) 19:40 https://doi.org/10.1186/s12935-019-0760-y Cancer Cell International PRIMARY RESEARCH Open Access A novel long non-coding RNA-KAT7 is low expressed in colorectal cancer and acts as a tumor suppressor Qingmei Wang1,2†, Rongzhang He1,3†, Tan Tan1,2†, Jia Li1, Zheng Hu1, Weihao Luo1, Lili Duan1, Wenna Luo1 and Dixian Luo1,2* Abstract Background: The abnormal expression of many long non-coding RNAs (lncRNAs) has been reported in the progres- sion of various tumors. However, the potential biological roles and regulatory mechanisms of long non-coding RNAs in the development of colorectal cancer (CRC) have not yet been fully elucidated. Therefore, it is crucial to identify that lncRNAs can be used for the clinical prevention and treatment of CRC. Methods: In our previous work, we identifcated a novel lncRNA, lncRNA-KAT7, and found that the expression of lncRNA-KAT7 in CRC tissues was signifcantly lower than that in matched normal intestinal tissues, and the expression in CRC cell lines was lower than that of normal intestinal epithelial cells (P < 0.05). Besides, the expression of lncRNA- KAT7 is negative associated with age, tumor size, tumor diferentiation, lymph node metastasis of CRC patients. The potential biological efects and molecular mechanisms of lncRNA-KAT7 in CRC were evaluated using a series of CCK-8 assay, clone formation assay, EdU proliferation assay, scratch determination, transwell determination, western blot analysis, and nude subcutaneous tumorigenesis model construction cell and animal experiments. Results: The expression of lncRNA-KAT7 in CRC tissues was lower than that in matched normal tissues and nor- mal intestinal epithelial cells (P < 0.05).
    [Show full text]
  • Lysine Acetyltransferase 8 Is Involved in Cerebral Development and Syndromic Intellectual Disability
    The Journal of Clinical Investigation RESEARCH ARTICLE Lysine acetyltransferase 8 is involved in cerebral development and syndromic intellectual disability Lin Li,1 Mohammad Ghorbani,1 Monika Weisz-Hubshman,2,3,4 Justine Rousseau,5 Isabelle Thiffault,6,7 Rhonda E. Schnur,8,9 Catherine Breen,10 Renske Oegema,11 Marjan M.M. Weiss,12 Quinten Waisfisz,12 Sara Welner,13 Helen Kingston,10 Jordan A. Hills,14 Elles M.J. Boon,12 Lina Basel-Salmon,2,3,4,15 Osnat Konen,4,16 Hadassa Goldberg-Stern,4,17 Lily Bazak,3,4 Shay Tzur,18,19 Jianliang Jin,1,20 Xiuli Bi,1 Michael Bruccoleri,1 Kirsty McWalter,9 Megan T. Cho,9 Maria Scarano,8 G. Bradley Schaefer,14 Susan S. Brooks,13 Susan Starling Hughes,6,7 K.L.I. van Gassen,11 Johanna M. van Hagen,12 Tej K. Pandita,21 Pankaj B. Agrawal,22 Philippe M. Campeau,5 and Xiang-Jiao Yang1,23 1Rosalind and Morris Goodman Cancer Research Centre and Department of Medicine, McGill University, Montreal, Quebec, Canada. 2Pediatric Genetics Unit, Schneider Children’s Medical Center of Israel, Petach Tikva, Israel. 3Raphael Recanati Genetic Institute, Rabin Medical Center, Petach Tikva, Israel. 4Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. 5Paediatric Department, CHU Sainte- Justine Hospital, University of Montreal, Quebec, Canada. 6Center for Pediatric Genomic Medicine & Division of Clinical Genetics, Children’s Mercy Hospital, Kansas City, Missouri, USA. 7Faculty of Medicine, University of Missouri-Kansas City, Kansas City, Missouri, USA. 8Division of Genetics, Cooper University Health Care, Camden, New Jersey, USA. 9GeneDx, Gaithersburg, Maryland, USA.
    [Show full text]
  • Chapter 2 Experimental Approaches Toward Histone Acetyltransferase Inhibitors As Therapeutics
    University of Groningen Kinetics and inhibition of enzymes in early stage drug discovery Wapenaar, Hannah IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2017 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Wapenaar, H. (2017). Kinetics and inhibition of enzymes in early stage drug discovery: A MOF and MIF symphony. Rijksuniversiteit Groningen. Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 27-09-2021 Chapter 2 Experimental approaches toward histone acetyltransferase inhibitors as therapeutics Part of this chapter was published in: Wapenaar H, Dekker FJ. Chapter 36. experimental approaches toward histone acetyltransferase inhibitors as therapeutics. In: Tollefsbol T, ed. Medical epigenetics. 1st ed. Elsevier; 2016:685-704. Experimental approaches towards histone acetyltransferase inhibitors as therapeutics The “histone code” Post-translational modifications (PTM) of chromatin-associated proteins (e.g.
    [Show full text]
  • Chromatin Unfolding by Cdt1 Regulates MCM Loading Via Opposing Functions of HBO1 and HDAC11-Geminin Philip G
    University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School 11-15-2010 Chromatin Unfolding by Cdt1 Regulates MCM Loading via Opposing Functions of HBO1 and HDAC11-Geminin Philip G. Wong University of South Florida Follow this and additional works at: http://scholarcommons.usf.edu/etd Part of the American Studies Commons Scholar Commons Citation Wong, Philip G., "Chromatin Unfolding by Cdt1 Regulates MCM Loading via Opposing Functions of HBO1 and HDAC11-Geminin" (2010). Graduate Theses and Dissertations. http://scholarcommons.usf.edu/etd/3696 This Dissertation is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. Chromatin Unfolding by Cdt1 Regulates MCM Loading via Opposing Functions of HBO1 and HDAC11-Geminin by Philip G. Wong A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy Department of Cancer Biology College of Arts and Sciences University of South Florida Major Professor: Mark G. Alexandrow, Ph.D. Lori A. Hazlehurst, Ph.D. Gary W. Reuther, Ph.D. Edward Seto, Ph.D. Date of Approval: November 15, 2010 Keywords: Cdt1, HDAC11, HBO1, chromatin, DNA replication Copyright © 2010, Philip G. Wong DEDICATION To my mother, Ming Hsieh and my father, Quon Wong And to my wife, Nancy Parquet ACKNOWLEDGEMENTS I would first like to thank Dr. Mark Alexandrow for his mentorship and guidance. I am grateful that he has put forth the effort to teach me what it means to become a scientist and I feel extremely fortunate to have been a member of his lab.
    [Show full text]
  • Exploring the Roles of KAT2A and KAT2B in Keratinocyte Biology
    Exploring the Roles of KAT2A and KAT2B in Keratinocyte Biology A thesis submitted to the University of Manchester for the degree of Doctor of Philosophy in the Faculty of Biology, Medicine and Health. School of Medical Sciences 2019 Benjamin W. Walters 2 LIST OF CONTENTS LIST OF FIGURES ...................................................................................................................... 7 LIST OF TABLES ...................................................................................................................... 11 LIST OF ABBREVIATIONS ...................................................................................................... 12 ABSTRACT ............................................................................................................................... 15 DECLARATION ........................................................................................................................ 16 COPYRIGHT STATEMENT ...................................................................................................... 16 ACKNOWLEDGMENTS ........................................................................................................... 17 CHAPTER 1 - INTRODUCTION 1.1 Human Skin Anatomy and Physiology................................................................................... 19 1.1.1 The Subcutaneous Tissue ........................................................................................ 19 1.1.2 The Dermis ...........................................................................................................
    [Show full text]