Catalog # Aliquot Size K316-911-05 3 x 5 nmol K316-911-20 3 x 20 nmol K316-911-50 3 x 50 nmol KAT7 (MYST2) siRNA Set I siRNA duplexes targeted against three exon regions Catalog # K316-911 Lot # Z2049-44 Specificity Formulation KAT7 (MYST2) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human KAT7 (MYST2) expression. shipped at room temperature. Product Description Reconstitution Protocol KAT7 (MYST2) siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human KAT7 collect lyophilized siRNA at the bottom of the tube. (MYST2) mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The gene accession number is NM_007067. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot KAT7; MYST2; HBO1; HBOA 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or KAT7 (MYST2), Active K316-380G below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background KAT7 or K (lysine) acetyltransferase 5 is a signaling protein KAT7 (MYST2) siRNA Set I that belongs to the MYST family of histone acetyl siRNA duplexes targeted against three exon regions transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein which play important roles in Catalog Number K316-911 regulating chromatin remodeling, transcription and other Specific Lot Number Z2049-44 nuclear processes by acetylating histone and nonhistone Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol proteins. KAT7 acts as a transcriptional inhibitor and Format Lyophilized powder inhibited AR-mediated transactivation of reporter Stability 1yr at -70oC from date of shipment constructs in CV-1 and PC-3 cells (1). KAT7 is part of a Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is multisubunit complex that can acetylate histones H3 and recommended that the lyophilized H4 (2). and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can References undergo up to 50 freeze-thaw cycles 1. Sharma, M. et.al: Androgen receptor interacts with a without significant degradation. For long-term storage, it is recommended novel MYST protein, HBO1. J. Biol. Chem. 275: 35200- that the siRNA is stored at -70oC. For 35208, 2000. most favorable performance, avoid 2. Iizuka, M. et.al: Histone acetyltransferase HBO1 repeated handling and multiple freeze/thaw cycles. interacts with the ORC1 subunit of the human initiator protein. J. Biol. Chem. 274: 23027-23034, 1999. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .