DOCSLIB.ORG

Ralstonia Solanacearum Management

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Ralstonia Solanacearum Management BACTERIAL WILT MANAGEMENT: A PREREQUISITE FOR A POTATO SEED CERTIFICATION PROGRAM IN MALI by Aissata Traore Thera A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science In Plant Pathology MONTANA STATE UNIVERSITY Bozeman, Montana November 2007 © COPYRIGHT by Aissata Traore Thera 2007 All Rights Reserved ii APPROVAL of a thesis submitted by Aissata Traore Thera This thesis has been read by each member of the thesis committee and has been found to be satisfactory regarding content, English usage, format, citations, bibliographic style, and consistency, and is ready for submission to the Division of Graduate Education. Dr. Barry J. Jacobsen Approved for the Department of Plant Sciences and Plant Pathology Dr. John E. Sherwood Approved for the Division of Graduate Education Dr. Carl A. Fox iii STATEMENT OF PERMISSION TO USE In presenting this thesis in partial fulfillment of the requirements for a master’s degree at Montana State University, I agree that the Library shall make it available to borrowers under rules of the Library. If I have indicated my intention to copyright this thesis by including a copyright notice page, copying is allowable only for scholarly purposes, consistent with “fair use” as prescribed in the U.S. Copyright Law. Requests for permission for extended quotation from or reproduction of this thesis in whole or in parts may be granted only by the copyright holder. Aissata Traore Thera November 2007 . iv DEDICATION To my son Sinaly Badra Thera With my unconditional love in the blessing from God, as a symbol of my determination to improve your courageous life of disablement. v ACKNOWLEDGMENTS I feel deeply thankful to the United States Agency for International Development (USAID) -Washington that provided my training funding through Higher Education for Development (HED), particularly the Principle Investigator, Dr. Florence Dunkel, who gave me the opportunity and the passion to study at Montana State University through her HED Grant; To Dr Barry Jacobsen my advisor for his guidance and his encouragement to help me getting my degree and Dr Nina Zidack for her availability. I would also like to thank Dr Gamby Kadiatou Toure and my home Institute IER in Mali (Institut de l’Economie Rurale) for their constant support; Montana State University for the tuition, and other facilities they provided me during the training; and the,Institut Polytechnique Rural/Institut de Formation et Recherche Appliquée (IPR/IFRA), for their collaboration during the research part of my thesis. I appreciate the precious help from the International Center of Potato CIP (Centro International de la Papa), the Montana Potato Lab group at MSU, PEO, a philanthropic organization for women in higher education and all the Department of Plant Sciences Plant Pathology from the Department Head, to all the professors and assistants who taught me and my colleagues in the laboratories: thanks for your advice and your answers to my multiple questions and concerns. I will be always grateful to my wonderful family for their courage and the love they provided to me all the time I have been far from home and to all my friends in Bozeman that have been always around supportting me so that my dream became reality. vi TABLE OF CONTENTS 1 INTRODUCTION ........................................................................................................ 1 Bacterial Wilt and Potato in Mali ................................................................................. 1 2 LITERATURE REVIEW............................................................................................. 8 Potato History and Crop Situation in Mali ................................................................... 8 Potato Crop in Mali: History and Trends...................................................................... 8 Zones of Production................................................................................................... 9 Sikasso ................................................................................................................... 9 Bamako and Baguineda. ....................................................................................... 10 Kati and Koulikoro Area Near Bamako................................................................ 11 Other Areas ..........................................................................................................11 Production................................................................................................................ 11 Seed Potato: Needs and Tentative Production............................................................ 14 Bacterial Wilt Situation............................................................................................... 16 Bacterial Wilt Worldwide........................................................................................ 16 Bacterial Wilt in Developing Countries and Africa................................................. 18 Bacterial Wilt in Mali .............................................................................................. 18 Important Diseases Found on Potato Crop in Mali..................................................... 20 Viral Diseases.......................................................................................................... 20 Potato Virus S. ...................................................................................................... 20 Potato Virus X....................................................................................................... 20 Potato Virus Y ...................................................................................................... 21 Potato Leaf Roll Virus . ........................................................................................ 21 Fungal Diseases....................................................................................................... 22 Pythium ultimum var. ultimum (Trow.) ................................................................ 22 Fusarium wilt- caused by Fusarium spp............................................................... 23 Rhizoctonia solani (Kuhn) . .................................................................................. 23 White Mold caused by Sclerotium rolfsii Sacc..................................................... 23 Early Blight caused by Alternaria solani (Ellis and G. Martin)........................... 24 Fusarium Dry Rot and Seed Decay caused by Fusarium solani Sacc. ............... 25 Bacterial Diseases on Potato in in Mali................................................................... 25 Soft Rot Disease of Tubers and Stems ................................................................. 25 Blackleg caused by Erwinia carotovora subsp. atroseptica (Van Hall) Dye (Synonym Pectobacterium atrosepticum) ........................................................... 26 Nematodes ............................................................................................................... 27 Bacterial Wilt or Brown Rot Disease Caused by R. solanacerum.............................. 28 Economic Importance of the Disease ...................................................................... 29 Taxonomic Position................................................................................................. 30 Morphological Aspect of the Bacteria..................................................................... 30 Classification........................................................................................................... 31 vii TABLE OF CONTENTS CONTINUED Genus....................................................................................................................... 35 Genomic Organization of the Bacteria .................................................................... 35 Pathogenicity........................................................................................................... 36 Pathogene Biology and Mode of Infection.............................................................. 37 Pathogen Ecology.................................................................................................... 38 Epidemiology.............................................................................................................. 38 Pathogen Distribution.............................................................................................. 38 Plant Host................................................................................................................. 42 Pathogen Survival.................................................................................................... 44 Pathogen Spread...................................................................................................... 47 Disease Symptoms and Diagnostics on Primary Hosts .............................................. 48 Potato....................................................................................................................... 48 Tomato..................................................................................................................... 49 Pelargonium............................................................................................................. 49 Tobacco.................................................................................................................... 49 Banana....................................................................................................................
Load more

Recommended publications
  • Fusarium Wilt of Bananas: a Review of Agro-Environmental Factors in the Venezuelan Production System Affecting Its Development
    agronomy Perspective Fusarium Wilt of Bananas: A Review of Agro-Environmental Factors in the Venezuelan Production System Affecting Its Development Barlin O. Olivares 1,*, Juan C. Rey 2 , Deyanira Lobo 2 , Juan A. Navas-Cortés 3 , José A. Gómez 3 and Blanca B. Landa 3,* 1 Programa de Doctorado en Ingeniería Agraria, Alimentaria, Forestal y del Desarrollo Rural Sostenible, Campus Rabanales, Universidad de Córdoba, 14071 Cordoba, Spain 2 Facultad de Agronomía, Universidad Central de Venezuela, Maracay 02105, Venezuela; [email protected] (J.C.R.); [email protected] (D.L.) 3 Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, 14004 Cordoba, Spain; [email protected] (J.A.N.-C.); [email protected] (J.A.G.) * Correspondence: [email protected] (B.O.O.); [email protected] (B.B.L.) Abstract: Bananas and plantains (Musa spp.) are among the main staple of millions of people in the world. Among the main Musaceae diseases that may limit its productivity, Fusarium wilt (FW), caused by Fusarium oxysporum f. sp. cubense (Foc), has been threatening the banana industry for many years, with devastating effects on the economy of many tropical countries, becoming the leading cause of changes in the land use on severely affected areas. In this article, an updated, reflective and practical review of the current state of knowledge concerning the main agro-environmental factors Citation: Olivares, B.O.; Rey, J.C.; that may affect disease progression and dissemination of this dangerous pathogen has been carried Lobo, D.; Navas-Cortés, J.A.; Gómez, J.A.; Landa, B.B. Fusarium Wilt of out, focusing on the Venezuelan Musaceae production systems.
    [Show full text]
  • Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management G
    Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management G. Blomme, M. Dita, K. S. Jacobsen, L. P. Vicente, A. Molina, W. Ocimati, Stéphane Poussier, Philippe Prior To cite this version: G. Blomme, M. Dita, K. S. Jacobsen, L. P. Vicente, A. Molina, et al.. Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management. Frontiers in Plant Science, Frontiers, 2017, 8, pp.1-25. 10.3389/fpls.2017.01290. hal-01608050 HAL Id: hal-01608050 https://hal.archives-ouvertes.fr/hal-01608050 Submitted on 28 Aug 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License fpls-08-01290 July 22, 2017 Time: 11:6 # 1 REVIEW published: 20 July 2017 doi: 10.3389/fpls.2017.01290 Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management Guy Blomme1*, Miguel Dita2, Kim Sarah Jacobsen3, Luis Pérez Vicente4, Agustin
    [Show full text]
  • Pastoralism and Security in West Africa and the Sahel
    Pastoralism and Security in West Africa and the Sahel Towards Peaceful Coexistence UNOWAS STUDY 1 2 Pastoralism and Security in West Africa and the Sahel Towards Peaceful Coexistence UNOWAS STUDY August 2018 3 4 TABLE OF CONTENTS Abbreviations p.8 Chapter 3: THE REPUBLIC OF MALI p.39-48 Acknowledgements p.9 Introduction Foreword p.10 a. Pastoralism and transhumance UNOWAS Mandate p.11 Pastoral Transhumance Methodology and Unit of Analysis of the b. Challenges facing pastoralists Study p.11 A weak state with institutional constraints Executive Summary p.12 Reduced access to pasture and water Introductionp.19 c. Security challenges and the causes and Pastoralism and Transhumance p.21 drivers of conflict Rebellion, terrorism, and the Malian state Chapter 1: BURKINA FASO p.23-30 Communal violence and farmer-herder Introduction conflicts a. Pastoralism, transhumance and d. Conflict prevention and resolution migration Recommendations b. Challenges facing pastoralists Loss of pasture land and blockage of Chapter 4: THE ISLAMIC REPUBLIC OF transhumance routes MAURITANIA p.49-57 Political (under-)representation and Introduction passivity a. Pastoralism and transhumance in Climate change and adaptation Mauritania Veterinary services b. Challenges facing pastoralists Education Water scarcity c. Security challenges and the causes and Shortages of pasture and animal feed in the drivers of conflict dry season Farmer-herder relations Challenges relating to cross-border Cattle rustling transhumance: The spread of terrorism to Burkina Faso Mauritania-Mali d. Conflict prevention and resolution Pastoralists and forest guards in Mali Recommendations Mauritania-Senegal c. Security challenges and the causes and Chapter 2: THE REPUBLIC OF GUINEA p.31- drivers of conflict 38 The terrorist threat Introduction Armed robbery a.
    [Show full text]
  • Appraisal Report Kankan-Kouremale-Bamako Road Multinational Guinea-Mali
    AFRICAN DEVELOPMENT FUND ZZZ/PTTR/2000/01 Language: English Original: French APPRAISAL REPORT KANKAN-KOUREMALE-BAMAKO ROAD MULTINATIONAL GUINEA-MALI COUNTRY DEPARTMENT OCDW WEST REGION JANUARY 1999 SCCD : N.G. TABLE OF CONTENTS Page PROJECT INFORMATION BRIEF, EQUIVALENTS, ACRONYMS AND ABBREVIATIONS, LIST OF ANNEXES AND TABLES, BASIC DATA, PROJECT LOGICAL FRAMEWORK, ANALYTICAL SUMMARY i-ix 1 INTRODUCTION.............................................................................................................. 1 1.1 Project Genesis and Background.................................................................................... 1 1.2 Performance of Similar Projects..................................................................................... 2 2 THE TRANSPORT SECTOR ........................................................................................... 3 2.1 The Transport Sector in the Two Countries ................................................................... 3 2.2 Transport Policy, Planning and Coordination ................................................................ 4 2.3 Transport Sector Constraints.......................................................................................... 4 3 THE ROAD SUB-SECTOR .............................................................................................. 5 3.1 The Road Network ......................................................................................................... 5 3.2 The Automobile Fleet and Traffic.................................................................................
    [Show full text]
  • Diseases of Trees in the Great Plains
    United States Department of Agriculture Diseases of Trees in the Great Plains Forest Rocky Mountain General Technical Service Research Station Report RMRS-GTR-335 November 2016 Bergdahl, Aaron D.; Hill, Alison, tech. coords. 2016. Diseases of trees in the Great Plains. Gen. Tech. Rep. RMRS-GTR-335. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. 229 p. Abstract Hosts, distribution, symptoms and signs, disease cycle, and management strategies are described for 84 hardwood and 32 conifer diseases in 56 chapters. Color illustrations are provided to aid in accurate diagnosis. A glossary of technical terms and indexes to hosts and pathogens also are included. Keywords: Tree diseases, forest pathology, Great Plains, forest and tree health, windbreaks. Cover photos by: James A. Walla (top left), Laurie J. Stepanek (top right), David Leatherman (middle left), Aaron D. Bergdahl (middle right), James T. Blodgett (bottom left) and Laurie J. Stepanek (bottom right). To learn more about RMRS publications or search our online titles: www.fs.fed.us/rm/publications www.treesearch.fs.fed.us/ Background This technical report provides a guide to assist arborists, landowners, woody plant pest management specialists, foresters, and plant pathologists in the diagnosis and control of tree diseases encountered in the Great Plains. It contains 56 chapters on tree diseases prepared by 27 authors, and emphasizes disease situations as observed in the 10 states of the Great Plains: Colorado, Kansas, Montana, Nebraska, New Mexico, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming. The need for an updated tree disease guide for the Great Plains has been recog- nized for some time and an account of the history of this publication is provided here.
    [Show full text]
  • Dutch Elm Disease
    DUTCH ELM DISEASE WHAT IS THE THREAT: Dutch Elm disease (DED) is a fungal vascular wilt disease primarily vectored by the elm bark beetle but previously affected trees often infect adjacent tree through root grafts. This disease is fatal if not treated preventively. WHERE IS THE THREAT: The most susceptible elms are English, American and winged elms while Siberian and Chinese elms are considered resistant. Older cities in the mid-west and north-eastern US were once over-planted with these stately trees until the 1970’s when many succumbed to this Early wilt symptoms disease. SYMPTOMS: Infection begins after full leaf out in the spring and appears as faded leaves still attached to twigs. This rapid collapse of tissue is due to death of new xylem cells and loss of water and nutrients. Olive discoloration of new wood appears as ‘streaking’ along the grain when twigs are sampled. Entire branches will wilt throughout the upper canopy within 4-6 weeks and unchecked will lead to tree mortality. Note: squirrel damage can look similar but is identified by the stripped, shredded bark at the base of limbs. WHAT TO DO ABOUT IT: Cut stems showing staining Elms treated preventively with a systemic fungicide such as Propizol® have the best chance for success. However, trees with 15% symptoms or less can still be treated but use the high rate. Wait until all leaves are fully formed prior to treatment and follow the dilution rates for the best distribution. Root grafts between trees within 30 feet of one another should be disrupted by trenching where practical.
    [Show full text]
  • Press Release
    PRESS RELEASE Symbol: "NAC" Toronto Stock Exchange North Atlantic acquires the Kourouba gold project in Mali Toronto, Canada, February 5, 2007: North Atlantic Resources Ltd. (“the Company”) reports: North Atlantic Resources is pleased to announce that it has acquired an option on the 185 square kilometer Kourouba gold exploration permit in southern Mali. The Kourouba project is located in the Koulikoro Region, 150 kilometers southeast of the capital, Bamako. The property covers a strike length of 14 kilometers of prospective stratigraphy in the Birimian Yanfolila greenstone belt. Under the terms of an option agreement, the Company can acquire the Kourouba exploration permit by paying an initial payment of US$60,000, US$100,000 on the first anniversary, US$140,000 on the second anniversary, and US$200,000 on the third anniversary, for a total of US$500,000. The property is subject to a 2.5% Net Smelter Returns Royalty which can be purchased any time by the Company for US$5.5 million. In the central part of the property, a previously-completed soil sampling program, at a density of 500 meters by 200 meters, defined a north-trending, continuous, gold in soil anomaly, with samples containing at least 100 parts per billion gold over a nominal strike length of 2,500 meters and a peak value of 1,420 parts per billion (1.42 grams per tonne gold). Follow-up soil sampling, completed by the vendor of the property, confirmed the presence of gold in termite mound samples, indicating that the gold in soil anomaly is probably coincident with gold in saprolite and bedrock below the anomaly.
    [Show full text]
  • Verticillium Wilt of Trees and Shrubs
    Dr. Sharon M. Douglas Department of Plant Pathology and Ecology The Connecticut Agricultural Experiment Station 123 Huntington Street, P. O. Box 1106 New Haven, CT 06504 Phone: (203) 974-8601 Fax: (203) 974-8502 Founded in 1875 Email: [email protected] Putting science to work for society Website: www.ct.gov/caes VERTICILLIUM WILT OF ORNAMENTAL TREES AND SHRUBS Verticillium wilt is a common disease of a wide variety of ornamental trees and shrubs throughout the United States and Connecticut. Maple, smoke-tree, elm, redbud, viburnum, and lilac are among the more important hosts of this disease. Japanese maples appear to be particularly susceptible and often collapse shortly after the disease is detected. Plants weakened by root damage from drought, waterlogged soils, de-icing salts, and other environmental stresses are thought to be more prone to infection. Figure 1. Japanese maple with acute symptoms of Verticillium wilt. Verticillium wilt is caused by two closely related soilborne fungi, Verticillium dahliae They also develop a variety of symptoms and V. albo-atrum. Isolates of these fungi that include wilting, curling, browning, and vary in host range, pathogenicity, and drying of leaves. These leaves usually do virulence. Verticillium species are found not drop from the plant. In other cases, worldwide in cultivated soils. The most leaves develop a scorched appearance, show common species associated with early fall coloration, and drop prematurely Verticillium wilt of woody ornamentals in (Figure 2). Connecticut is V. dahliae. Plants with acute infections start with SYMPTOMS AND DISEASE symptoms on individual branches or in one DEVELOPMENT: portion of the canopy.
    [Show full text]
  • Ralstonia Solanacearum Race 3 Biovar 2 OriginalWebpage(SeeLink AtTheEndOfTheDocument)
    USDA-NRI Project: R. solanacearum race 3 biovar 2: detection, exclusion and analysis of a Select Agent Educational modules Ralstonia solanacearum race 3 biovar 2 Original webpage (see link at the end of the document) Author : Patrice G. Champoiseau of University of Florida Reviewers : Caitilyn Allen of University of Wisconsin; Jeffrey B. Jones , Carrie Harmon and Timur M. Momol of University of Florida Publication date : September 1 2, 2008 Supported by : The United States Department of Agriculture - National Research Initiative Program (2007 -2010) - See definitions of red-colored words in the glossary at the end of this document - Ralstonia solanacearum race 3 biovar 2 is the plant pathogen bacterium that causes brown rot (or bacterial wilt) of potato, Southern wilt of geranium, and bacterial wilt of tomato. R. solanacearum race 3 biovar 2 occurs in highlands in the tropics and in subtropical and some warm-temperate areas throughout the world. It has also occurred in cold-temperate regions in Europe, where several outbreaks of brown rot of potato have been reported in the last 30 years. It has been reported in more than 30 countries and almost all continents. In the United States, several introductions of R. solanacearum race 3 biovar 2 have already occurred as a result of importation of infested geranium cuttings from off-shore production sites, but the pathogen was apparently eradicated. However, because of the risk of its possible re-introduction through importation of infected plant material, and its potential to affect potato production in cold-temperate areas in the northern United States, R. solanacearum race 3 biovar 2 is considered a serious threat to the United States potato industry.
    [Show full text]
  • Genomic Plasticity of the Causative Agent of Melioidosis, Burkholderia Pseudomallei
    Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei Matthew T. G. Holdena, Richard W. Titballb,c, Sharon J. Peacockd,e, Ana M. Cerden˜ o-Ta´ rragaa, Timothy Atkinsb, Lisa C. Crossmana, Tyrone Pittf, Carol Churchera, Karen Mungalla, Stephen D. Bentleya, Mohammed Sebaihiaa, Nicholas R. Thomsona, Nathalie Basona, Ifor R. Beachamg, Karen Brooksa, Katherine A. Brownh, Nat F. Browng, Greg L. Challisi, Inna Cherevacha, Tracy Chillingwortha, Ann Cronina, Ben Crossetth, Paul Davisa, David DeShazerj, Theresa Feltwella, Audrey Frasera, Zahra Hancea, Heidi Hausera, Simon Holroyda, Kay Jagelsa, Karen E. Keithh, Mark Maddisona, Sharon Moulea, Claire Pricea, Michael A. Quaila, Ester Rabbinowitscha, Kim Rutherforda, Mandy Sandersa, Mark Simmondsa, Sirirurg Songsivilaik, Kim Stevensa, Sarinna Tumapae, Monkgol Vesaratchaveste, Sally Whiteheada, Corin Yeatsa, Bart G. Barrella, Petra C. F. Oystonb, and Julian Parkhilla,l aWellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom; bDefence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, United Kingdom; cDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom; dNuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, United Kingdom; eFaculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; fLaboratory of Hospital Infection, Division of Nosocomial Infection Prevention and Control, Central Public Health Laboratory, London NW9 5HT, United Kingdom; gSchool of Health Science, Griffith University, Gold Coast, Queensland 9726, Australia; hDepartment of Biological Sciences, Centre for Molecular Microbiology and Infection, Flowers Building, Imperial College, London SW7 2AZ, United Kingdom; iDepartment of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom; jU.S.
    [Show full text]
  • TAL Effectors Are Remote Controls for Gene Activation Heidi Scholze and Jens Boch
    Available online at www.sciencedirect.com TAL effectors are remote controls for gene activation Heidi Scholze and Jens Boch TAL (transcription activator-like) effectors constitute a novel typically 34 amino acids (aa) long, but also repeat types of class of DNA-binding proteins with predictable specificity. They 30–42 aa can be found ([2], Figure 2). The last repeat is are employed by Gram-negative plant-pathogenic bacteria of only a half repeat. Repeat-to-repeat variations are limited the genus Xanthomonas which translocate a cocktail of to a few aa positions including two hypervariable residues different effector proteins via a type III secretion system (T3SS) at positions 12 and 13 per repeat. Typically, TALs differ into plant cells where they serve as virulence determinants. in their number of repeats while most contain 15.5–19.5 Inside the plant cell, TALs localize to the nucleus, bind to target repeats [2]. The repeat domain determines the specificity promoters, and induce expression of plant genes. DNA-binding of TALs which is mediated by selective DNA binding specificity of TALs is determined by a central domain of tandem [7,8]. TAL repeats constitute a novel type of DNA- repeats. Each repeat confers recognition of one base pair (bp) binding domain [7] distinct from classical zinc finger, in the DNA. Rearrangement of repeat modules allows design of helix–turn–helix, and leucine zipper motifs. proteins with desired DNA-binding specificities. Here, we summarize how TAL specificity is encoded, first structural data The recent understanding of the DNA-recognition speci- and first data on site-specific TAL nucleases.
    [Show full text]
  • Sonication Contribution to Identifying Prosthetic Joint Infection With
    Birlutiu et al. BMC Musculoskeletal Disorders (2017) 18:311 DOI 10.1186/s12891-017-1678-y CASE REPORT Open Access Sonication contribution to identifying prosthetic joint infection with Ralstonia pickettii: a case report and review of the literature Rares Mircea Birlutiu1*, Mihai Dan Roman2, Razvan Silviu Cismasiu3, Sorin Radu Fleaca2, Crina Maria Popa4, Manuela Mihalache5 and Victoria Birlutiu6 Abstract Background: In the context of an increase number of primary and revision total hip and total knee arthroplasty performed yearly, an increased risk of complication is expected. Prosthetic joint infection (PJI) remains the most common and feared arthroplasty complication. Ralstonia pickettii is a Gram-negative bacterium, that has also been identified in biofilms. It remains an extremely rare cause of PJI. There is no report of an identification of R. pickettii on an extracted spacer loaded with antibiotic. Case presentation: We present the case of an 83-years-old Caucasian male patient, that underwent a right cemented total hip replacement surgery. The patient is diagnosed with an early PJI with no isolated microorganism. A debridement and change of mobile parts is performed. At the beginning of 2016, the patient in readmitted into the Orthopedic Department for sever, right abdominal and groin pain and elevated serum erythrocyte sedimentation rate and C-reactive protein. A joint aspiration is performed with a negative microbiological examination. A two-stage exchange with long interval management is adopted, and a preformed spacer loaded with gentamicin was implanted. In July 2016, based on the proinflammatory markers evolution, a shift a three-stage exchange strategy is decided. In September 2016, a debridement, and changing of the preformed spacer loaded with gentamicin with another was carried out.
    [Show full text]