Supplementary Tables
Total Page:16
File Type:pdf, Size:1020Kb
Table 1. Primer Sequences HUGO Forward Reverse RPL13A 5'CCTGGAGGAGAAGAGGAAAGAGA 3' 5'TTGAGGACCTCTGTGTATTTGTCAA 3' TFF1 5'GTGCAAATAAGGGCTGCTGTTT 3' 5'TGGAGGGACGTCGATGGTATTA 3' FOLH1 5'CAAGCAGCCACAACAAGTATGC 3' 5'GAAGGGTCCACTTTGCTTTCAA 3' FKBP5 5'AAAAGGCCACCTAGCTTTTTGC 3' 5'CCCCCTGGTGAACCATAATACA 3' TMPRSS2 5'CCATTTGCAGGATCCGTCTG 3' 5'GGATGTGTCTTGGGGAGCAA 3' SPON2 5'CACGTGGTTGCAGATACCTCAG 3' 5'ACCGCCCCATTTATTCACTTCT 3' NDRG1 5'GGCTGCCAGGTTCCTGTACTACT 3' 5'GGTCCCAGTGGCTGACTTACAA 3' IGFBP5 5'TCTCCTCTCTACCGCCACTGTC 3' 5'TAGGCAACTCGGAATCTGAAGG 3' MME 5'CACAGCTGGAGCTCTCAAACTAA 3' 5'TTTTGTATTTTTGAGACTGGAAACTGA 3' PPAP2A 5' ATGCCTCTTGGATGCACACTTT 3' 5'ATACAGGTGGGGCACTGTTTTG 3' VEGF 5' TTTCCAATCTCTCTCTCCCTGAT 3' 5'GAGGGCAGAGCTGAGTGTTAGC 3' AR 5'ATCCTCATATGGCCCAGTGTCAAG 3' 5'GCTCTCTAAACTTCCCGTGGCATA 3' PSA 5'GCATGGGATGGGGATGAAGTAAG 3' 5'CATCAAATCTGAGGGTTGTCTGGA 3' ACPP 5'CCCTCCTGGTTGGTCACAGAAT 3' 5'GAAAATGGACCCAAGGACAAACA 3' NKX3.1 5'AACCATTTCACCCAGACAGCCT 3' 5'TGTGACAGATTGGAGCAGGGTT 3' Seladin 5'TAAACAGTGCCATTGTTTTGCC 3' 5'CCCAGTGGCTTAGGGTTGG 3' Clusterin 5' GAGCTCTGCACGTCACCAAGTA 3' 5' TTCTTCCCATGAGCAGCAGAGT 3' TABLE 2. Genes differentially regulated >1.5 fold in Acyline Treated Prostate Epithelial Samples (FDR<5%) HUGO NAME GENBANK FOLD HUGO NAME GENBANK FOLD Stress Response Protease/Protease Inhibitor HSPD1 Heat shock 60kDa protein 1 (chaperonin) BQ879606 1.9 FOLH1 Folate hydrolase (prostate-specific membrane antigen) 1 AF176574 2.8 HSPCA Heat shock 90kDa protein 1 alpha-like 3 BC001695 1.7 LOC83690 CocoaCrisp AF142573 1.8 LGALS3BP Lectin galactoside-binding soluble 3 binding protein BQ883924 -1.6 DMXL1 Dmx-like 1 NM_005509 1.7 ORM2 Orosomucoid 2 BC015964 -3.1 CTSD Cathepsin D (lysosomal aspartyl protease) AL557431 -1.5 NAALADL1 N-acetylated alpha-linked acidic dipeptidase-like 1 CA308506 -1.5 DNA Synthesis/Repair TMPRSS2 Transmembrane protease serine 2 NM_005656 -2.4 MRE11A MRE11 meiotic recombination 11 homolog A (S. cerevisiae) BQ890553 1.7 MME Membrane metallo-endopeptidase (neutral endopeptidase enkephalinase CALLA CDAL833459 -2.6 RRM1 Ribonucleotide reductase M1 polypeptide AK122695 1.6 ATM Ataxia telangiectasia mutated (includes complementation groups A C and D) U67093 1.6 Immune/Inflammatory Response PLA2G7 Phospholipase A2 group VII (platelet-activating factor acetylhydrolase plasma) BC038452 1.8 Transcription Regulation CD59 CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5 EJ16 EJ30BM550387 1.5 MATR3 Matrin 3 AK001388 1.9 MUC13 Mucin 13 epithelial transmembrane AK000070 -1.6 HNRPF Heterogeneous nuclear ribonucleoprotein F BC016736 1.8 CCL20 Chemokine (C-C motif) ligand 20 D86955 -1.8 ETS1 V-ets erythroblastosis virus E26 oncogene homolog 1 (avian) CN357911 1.8 CCL1 Chemokine (C-C motif) ligand 1 BX093610 -2.6 ZA20D2 Zinc finger A20 domain containing 2 CR612072 1.7 SPON2 Spondin 2 extracellular matrix protein AK024499 -5.7 ZNF292 Zinc finger protein 292 AB011102 1.7 HNRPR Heterogeneous nuclear ribonucleoprotein R AL548404 1.7 Signal Transduction BTF3 Basic transcription factor 3 BX359147 1.6 SFN Unconfirmed: Stratifin AI356363 1.9 HRB2 HIV-1 rev binding protein 2 BC005225 1.6 CALM2 Calmodulin 2 (phosphorylase kinase delta) BM508076 1.7 HNRPU Heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) CR614336 1.6 TSPAN8 tetraspanin 8 NM_004616 1.7 COMMD6 COMM domain containing 6 BU075627 1.6 TM4SF1 transmembrane 4 L six family member 1 AK130073 1.7 IRX3 Iroquois homeobox protein 3 BM980278 1.5 MAP3K2 Mitogen-activated protein kinase kinase kinase 2 AK093893 1.7 CHD2 Chromodomain helicase DNA binding protein 2 AF006514 1.5 Homo sapiens clone IMAGE:5272626 mRNA CA397791 1.6 HIST2H2AA Histone 2 H2aa AV721202 -1.5 PHIP Pleckstrin homology domain interacting protein BC008909 1.6 EFHD1 EF hand domain containing 1 CR601176 -1.5 SPRED2 Sprouty-related EVH1 domain containing 2 CR609442 1.6 CIRBP Cold inducible RNA binding protein BM423685 -1.6 SOCS3 Suppressor of cytokine signaling 3 BF514189 1.6 NUCKS Nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate CR597568 -1.7 SH3BP5 SH3-domain binding protein 5 (BTK-associated) AI003583 1.5 NCOA4 Nuclear receptor coactivator 4 CR599220 -2.1 PRKAR1A Protein kinase cAMP-dependent regulatory type I alpha (tissue specific extinguisher 1)BF940164 1.5 MAF V-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) BE674528 -2.3 SH3BP4 SH3-domain binding protein 4 AF015043 1.5 KIT V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog X06182 -1.5 Translation - Protein Synthesis CAMK1 Calcium/calmodulin-dependent protein kinase I CR607002 -1.5 SMN1 Survival of motor neuron 1, telomeric BF343715 2.1 FARP1 FERM RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) AB008430 -1.5 RPS15A Ribosomal protein S15a CN351295 1.7 MYLK Myosin light polypeptide kinase NM_053031 -2.0 DHX36 DEAH (Asp-Glu-Ala-His) box polypeptide 36 AJ577134 1.7 FKBP5 FK506 binding protein 5 BU618502 -2.7 NACA Nascent-polypeptide-associated complex alpha polypeptide BM470033 1.6 PSCA Prostate stem cell antigen AJ297436 -3.2 SKP1A S-phase kinase-associated protein 1A (p19A) CN409596 1.6 RPL31 Ribosomal protein L31 CN269893 1.5 Structure/Motility/Adhesion RPL17 Ribosomal protein L17 BM562574 1.5 ACTR10 Actin-related protein 10 homolog (S. cerevisiae) BM921747 1.6 RPS24 Ribosomal protein S24 CN369064 1.5 INA Internexin neuronal intermediate filament protein alpha CR591335 1.5 CHPPR Likely ortholog of chicken chondrocyte protein with a poly-proline region BM807090 1.5 CCT3 Chaperonin containing TCP1 subunit 3 (gamma) BP371798 1.5 HSRG1 HSV-1 stimulation-related gene 1 CK429627 1.5 Growth/Differentiation/Apoptosis CKB Creatine kinase brain BC004914 -1.6 SON SON DNA binding protein X63751 1.8 NID Nidogen (enactin) CR611614 -1.6 TP53INP1 Tumor protein p53 inducible nuclear protein 1 AB017926 1.5 TAGLN Transgelin CD050815 -1.6 TGFBR1 Transforming growth factor beta receptor I (activin A receptor type II-like kinase 53kDAI244728 1.5 FLJ10350 Hypothetical protein FLJ10350 BC027334 -1.6 BCAP31 B-cell receptor-associated protein 31 BM674821 -1.5 CSRP1 Cysteine and glycine-rich protein 1 AL545856 -1.6 FHL1 Four and a half LIM domains 1 BU151720 -1.5 DMN Desmuslin AL833262 -1.6 OGN Osteoglycin (osteoinductive factor mimecan) BU687437 -1.5 ACTA2 Actin alpha 2 smooth muscle aorta CR592949 -2.2 VEGF Unconfirmed: Vascular endothelial growth factor R19956 -1.6 LMAN1L Lectin mannose-binding 1 like AK074096 -2.4 CDC25B Cell division cycle 25B CR620818 -1.6 DCN Decorin CD245595 -2.5 EDN3 Endothelin 3 X52001 -2.1 MYBPC1 Myosin binding protein C slow type BF516586 -4.8 CTGF Connective tissue growth factor AI753440 -2.1 NBL1 Neuroblastoma suppression of tumorigenicity 1 BU734244 -2.4 Transport/Trafficking EGF Epidermal growth factor (beta-urogastrone) X04571 -2.6 TBCE Tubulin-specific chaperone e CR612606 1.8 IGFBP5 Insulin-like growth factor binding protein 5 NM_000599 -3.0 COG4 Component of oligomeric golgi complex 4 BC013347 1.8 NDRG1 N-myc downstream regulated gene 1 CR600627 -3.2 SLC25A17 Solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein 34kDa CR624010 1.7 TCP1 T-complex 1 BC000665 1.6 Metabolism SLC27A2 Solute carrier family 27 (fatty acid transporter) member 2 BM980042 1.6 ENTPD6 Ectonucleoside triphosphate diphosphohydrolase 6 (putative function) AK094030 -1.7 LOC168850 Hypothetical protein LOC168850 BC037348 1.6 PTGES2 Prostaglandin E synthase 2 NM_198940 -1.9 COPB Coatomer protein complex subunit beta CR605573 1.6 GSTM1 Glutathione S-transferase M1 NM_000561 -2.1 SNX14 Sorting nexin 14 AF121863 1.6 LOC152485 Hypothetical protein LOC152485 BC033655 -2.3 COPE Coatomer protein complex subunit epsilon BG680266 1.5 Metabolism - Carbohydrate RSN Restin (Reed-Steinberg cell-expressed intermediate filament-associated protein) M97501 1.5 TFF1 Trefoil factor 1 (breast cancer estrogen-inducible sequence expressed in) CK820254 3.8 ATP6V0D1 ATPase H+ transporting lysosomal 38kDa V0 subunit d isoform 1 BP261132 -1.5 PYGL Phosphorylase glycogen; liver (Hers disease glycogen storage disease type VI) CR594898 1.9 PACSIN2 Protein kinase C and casein kinase substrate in neurons 2 CR456536 -1.5 DPAGT1 Dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransfeCA429495 1.5 ABCC4 ATP-binding cassette sub-family C (CFTR/MRP) member 4 AY081219 -1.6 ALDH2 Aldehyde dehydrogenase 2 family (mitochondrial) CR609508 1.5 WIPI1 WD repeat domain, phosphoinositide interacting 1 BQ021751 -1.7 MAN1C1 Mannosidase alpha class 1C member 1 CR624971 1.5 SLC26A2 Solute carrier family 26 (sulfate transporter) member 2 BM979084 -1.7 DCXR Dicarbonyl/L-xylulose reductase BM795570 -1.5 SLC37A4 Solute carrier family 37 (glycerol-6-phosphate transporter) member 4 BI668967 -1.9 GPD1L Glycerol-3-phosphate dehydrogenase 1-like AK125404 -1.5 MT2A Metallothionein 2A BG925361 -2.8 SORD Sorbitol dehydrogenase BC025295 -1.7 Metabolism - Lipid/Sterol Unknown/Other FDFT1 Farnesyl-diphosphate farnesyltransferase 1 S76822 1.5 SCCPDH saccharopine dehydrogenase (putative) CR601818 2.0 TSTA3 Tissue specific transplantation antigen P35B BQ009612 -1.5 MALAT1 Metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) CA952436 1.8 ECH1 Enoyl Coenzyme A hydratase 1 peroxisomal BX353730 -1.5 FAM60A family with sequence similarity 60, member A BU170034 1.7 HSD11B2 Hydroxysteroid (11-beta) dehydrogenase 2 BX417858 -1.5 C6orf136 Chromosome 6 open reading frame 136 BQ954823 1.7 ACSL3 Acyl-CoA synthetase long-chain family member 3 AK023191 -1.7 HSPC148 Hypothetical protein HSPC148