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E-Catalog Version 19.0 E-Catalog Version 19.0 ChemGenes Corporation is now ISO 9001:2008 Certified Company Bio Technology Products & Manufacturer of DNA-RNA Intermediates 33 Industrial Way Wilmington, MA 01887 USA Tel: 978-694-4500 Fax: 978-694-4502 Toll Free: 800-762-9323 Email: [email protected] www.chemgenes.com Product Highlight Extensive Product Lines: DNA amidites: Bulk Quantities for therapeutics grade oligo synthesis Natural and modified DNA amidites Natural & modified RNA amidites : Bulk Quantities 2’-O-Methyl amidites: Bulk Quantities RNA Synthesis in Reverse Direction TOM amidites for RNA synthesis : Bulk Quantities 2’-O-ALE amidites 2’-O-PivOM amidites 2’-Fluoro amidites Non-Cleavable Inert Supports ETT & BMT : Bulk Quantities DDTT Sulfurizing Reagent UnyLinker Universal Support Akta Oligonucleotide Synthesizer Reagents N-Alkylated nucleosides and amidites 5-Azacytidine and 5-Aza-2’-deoxycytidine Thio uridines & thymidines nucleosides and amidites 6 & 5-FAM NHS Esters 6 & 5-FAM-(C-6 linker) amidites Biotin (BB)-(urea nitrogen protected) Supports & amidites Polyethylene glycol (PEG) (MW. 2000 and 4500) amidites 5’-MMTr nucleoside amidites (Purines: A & G) 8-Methyl dG & rG amidites 8-Oxo dA & dG amidites 5’-O-Methyl DNA amidites Fmoc Protected Nucleoside amidites 5-Formyl dU amidites Ammonia Free Deprotection Reagents Thiol modifiers (acyclic and cyclic) DNA and RNA Purification kit Toll Free: (800) 762-9323 Toll Free: 33 Industrial(800) Way, Wilmington, 762-9323 MA 01887 • T: (978) 694-4500 • F: (978) 694-4502 33 Industrial Way, Wilmington, MA 01887 • T: (978) 694-4500 • F: (978) 694-4502 www.chemgenes.com Email: [email protected] Experience Nucleic Acid Expertise Since 1981, the products and services provided by ChemGenes Corporation continue to accelerate the pace of DNA/RNA oligonucleotide synthesis, research, and develop- ment in the pharmaceutical and biotechnology industry. 33 Industrial Way Wilmington, MA 01887 Call: 978-694-4500, Toll Free: 800-762-9323 Fax: 978-694-4502 Email: [email protected] Visit us at: www.chemgenes.com www.chemgenes.com Email: [email protected] ChemGenes Corporation Background ChemGenes Corporation, a biotechnology company located in Wilmington, Massachusetts, has been a strong partner to researchers engaged in the field of DNA/RNA synthesis for more than 20 years. By starting out as a supplier of ‘Ultra Pure Products’ and then also by becoming an active Contract Researcher, ChemGenes Corporation has achieved unparalleled expertise in the field of DNA/RNA synthesis. Thus, all of us at ChemGenes have the opportunity and great pleasure to serve a vast number of scientists in the US and all over the world. ChemGenes helds a number of patents in the field of standard, modified, as well as backbone modified DNA/RNA synthesis products. ChemGenes has a worldwide licensing agreement on US patent No.s 4,415,732 and 4,458,066, pioneering patents on the discovery of DNA and RNA syn- thesis by Caruthers and Beaucage. We had another worldwide licensing agreement on US patent No. Re 34,069, the presently popular cyanoethyl (CEP) phosphoramidite version for DNA and RNA synthesis. ChemGenes product lines include phosphoramidites for DNA and RNA synthesis, Antisense phos- phoramidites, modified bases for DNA, as well as RNA modification. In addition, we produce a vari- ety of modified phosphoramidites for the introduction of chromophores and ligands. The availability of prepacked disposable columns of various pore sizes, loadings, low volume columns, ancillary reagents in configurations suitable for each synthesizer, and DNA purification cartridges is another way ChemGenes meets its customers’ needs. ChemGenes has a strong, dedicated, and talented team of professionals for the manufacturing of many natural and modified nucleoside bases. Included in these natural and modified bases are DMTr protected deoxy and ribo nucleosides, 2’-O-methyl and 2’-O-propargyl nucleosides, and silyl protected ribonucleosides. A large variety of reagents for the synthesis of DNA/RNA are also available. These include 5-thio- ethyltetrazole (ETT), benzylthiotetrazole (BTT/BMT), DMTr-chloride, MMTr-chloride, and several types of phosphorylating reagents such as cyanoethyl chloro phosphitylating reagent, cyanoethyl tetra iso- propyl phosphitylating reagent, cyanoethyl dichloro phosphitylating reagent and 5’, 3’-bis silylating reagent. As the market for oligonucleotides continues to grow, ChemGenes will stay committed to introduc- ing novel products, while maintaining its existing product mix. We also have the capacity to custom synthesize products on request. Our highly trained technical staff is always available and happy to help you with your special needs and demands in this area. For information regarding the full range of products and their applications, please call us at 978-694-4500 or toll-free 800-762-9323, fax: 978-694-4502, or e-mail: [email protected] i Toll Free: (800) 762-9323 33 Industrial Way, Wilmington, MA 01887 • T: (978) 694-4500 • F: (978) 694-4502 Special Gratitude The nucleoside phosphoramidites available in this catalog are the result of the classical research contri- bution by Professor M.H. Caruthers and colleagues (University of Colorado, Boulder, CO) (Beaucage S. L.; Caruthers, M. H. Tet. Lett., 1981, 22,1859-1862). Other enormous contributions in this field have been made by Professor K.K. Ogilvie and colleagues (Acadia University, Acadia, Canada), Professor R.L. Letsinger and col- leagues (Northwestern University, Evanston, IL), and Professor H. G. Khorana, Noble Laureate and colleagues (Massachusetts Institute of Technology, Cambridge, MA). Patents, Licensing & Trademarks Patent & Application Number Title of Patent Date Issued 7956169 Synthesis of novel azo-dyes and their use in oligonucleotide synthesis June 7, 2011 The invention provides a novel group of azo quencher compositions that are useful as quenchers of fluorescence and to methods for making and using them. The quenchers contain an azo bond and 1,3,3-trimethyl-2-methyleneindoline ring system. The quenchers can be derivatized to facilitate their conjugation to a variety of biologically relevant compounds, including lipids, nucleic acids, peptides, proteins, and the like. 7846436 Oligonucleotides and related compounds December 7, 2010 The present invention relates generally to oligonucleotides and more specifically to oligonucleotides which have a se- quence including at least two CpG dinucleotides and a prodrug of an antimetabolite. The prodrug can be part of a CpG dinucleotide or may be attached elsewhere on the oligonucleotide. 9493773 Synthesis of deuterated ribo nucleosides, N-protected phosphoramidites, and oligonucleotides App# CA2880496 Nov 15, 2016 App#CN104812770 App# EP2882769 App# WO2014022566 The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphorami- dites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined se-quences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses. 9718855 Nucleosides and oligonucleotides for studies on reversal of cytotoxic and mutagenic damage of DNA and as diagnosticstools Aug 1, 2016 The present invention is directed to n-alkylated synthetic nucleosides of high regiospecific purity and oligonucleotides that can be utilized for studies on reversal of cytotoxic and mutagenic DNA damage, and as diagnostic tools. App# CA2924186 Long RNA, Highly efficient synthesis of long RNA using Reverse App# CN105916873 Direction Approach Oct 29, 2015 App# WO2015039053 The present invention relates to novel process of reverse 5 ' 3 ' directed synthesis of RNA oligomers in the range of about 100-mer to about 200-mer has been developed and disclosed. Using that method demonstrated high quality RNA synthe- sis with coupling efficiency approaching 99%. ii www.chemgenes.com Email: [email protected] ChemGenes Corporation 9884885 Synthesis of labile base protected - modified deoxy & modified ribo nucleo- sides, corresonding phosphoramidites and supports and their use in high purity oligonucleotide synthesis Mar 15,2012 This invention relates to novel method of synthesis of RNA utilizing N-2-acetyl protected guanine as nucleoside base, nucleosides, succinates, phosphoramidites, corresponding solid supports that are suitable for oligo deoxy nucleosides and RNA oligonucleotide synthesis. Our discovery using N-acetyl protected guanine as nucleoside base protecting group, which is significantly faster base labile protecting group, yet significantly more stable than commonly utilized -2-isobutyryl guanosine is a novel approach to obtain highest purity oligonucleotides. This approach is designed to lead to very high purity and very clean oligonucleotide, after efficient removal of the protecting groups, including ace- tyl group from guanine and to produce high purity therapeutic grade DNA oligonucleotides, RNA oligonucleotides, diagnostic DNA, diagnostic RNA for microarray platform. The deprotection of acetyl protecting groups of the natural deoxy and ribonu-cleosides occurs under substantially reduced time in contact with mild deprotection conditions such as mild bases, sec-ondary amines for removal of such groups under such conditions would allows synthesis of various DNA and RNA of highest purity for diagnostics and therapeutic application. This approach is designed to lead to high purity large. 9605261 RNA synthesis—phosphoramidites
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