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E-Catalog Version 18.0 Release Date: 05-10-2013 E-Catalog Version 18.0 Release Date: 05-10-2013 ChemGenes Corporation is now ISO 9001:2008 Certified Company Bio Technology Products & Manufacturer of DNA-RNA Intermediates 33 Industrial Way Wilmington, MA 01887 USA Tel: 978-694-4500 Fax: 978-694-4502 Toll Free: 800-762-9323 Email: [email protected] www.chemgenes.com Product Highlight Extensive Product Lines: DNA amidites: Bulk Quantities for therapeutics grade oligo synthesis Natural and modified DNA amidites Natural & modified RNA amidites : Bulk Quantities 2’-O-Methyl amidites: Bulk Quantities RNA Synthesis in Reverse Direction TOM amidites for RNA synthesis : Bulk Quantities 2’-O-ALE amidites 2’-O-PivOM amidites 2’-Fluoro amidites Non-Cleavable Inert Supports ETT & BMT : Bulk Quantities DDTT Sulfurizing Reagent UnyLinker Universal Support Akta Oligonucleotide Synthesizer Reagents N-Alkylated nucleosides and amidites 5-Azacytidine and 5-Aza-2’-deoxycytidine Thio uridines & thymidines nucleosides and amidites 6 & 5-FAM NHS Esters 6 & 5-FAM-(C-6 linker) amidites Biotin (BB)-(urea nitrogen protected) Supports & amidites Polyethylene glycol (PEG) (MW. 2000 and 4500) amidites 5’-MMTr nucleoside amidites (Purines: A & G) 8-Methyl dG & rG amidites 8-Oxo dA & dG amidites 5’-O-Methyl DNA amidites Fmoc Protected Nucleoside amidites 5-Formyl dU amidites Ammonia Free Deprotection Reagents Thiol modifiers (acyclic and cyclic) DNA and RNA Purification kit Toll Free: (800) 762-9323 33 Industrial Way, Wilmington, MA 01887 • T: (978) 694-4500 • F: (978) 694-4502 www.chemgenes.com Email: [email protected] Experience Nucleic Acid Expertise Since 1981, the products and services provided by ChemGenes Corporation continue to accelerate the pace of DNA/RNA oligonucleotide synthesis, research, and develop- ment in the pharmaceutical and biotechnology industry. 33 Industrial Way Wilmington, MA 01887 Call: 978-694-4500, Toll Free: 800-762-9323 Fax: 978-694-4502 Email: [email protected] Visit us at: www.chemgenes.com ChemGenes Corporation Background ChemGenes Corporation, a biotechnology company located in Wilmington, Massachusetts, has been a strong partner to researchers engaged in the field of DNA/RNA synthesis for more than 20 years. By starting out as a supplier of ‘Ultra Pure Products’ and then also by becoming an active Contract Researcher, ChemGenes Corporation has achieved unparalleled expertise in the field of DNA/RNA synthesis. Thus, all of us at ChemGenes have the opportunity and great pleasure to serve a vast number of scientists in the US and all over the world. ChemGenes helds a number of patents in the field of standard, modified, as well as backbone modified DNA/RNA synthesis products. ChemGenes has a worldwide licensing agreement on US patent No.s 4,415,732 and 4,458,066, pioneering patents on the discovery of DNA and RNA syn- thesis by Caruthers and Beaucage. We had another worldwide licensing agreement on US patent No. Re 34,069, the presently popular cyanoethyl (CEP) phosphoramidite version for DNA and RNA synthesis. ChemGenes product lines include phosphoramidites for DNA and RNA synthesis, Antisense phos- phoramidites, modified bases for DNA, as well as RNA modification. In addition, we produce a vari- ety of modified phosphoramidites for the introduction of chromophores and ligands. The availability of prepacked disposable columns of various pore sizes, loadings, low volume columns, ancillary reagents in configurations suitable for each synthesizer, and DNA purification cartridges is another way ChemGenes meets its customers’ needs. ChemGenes has a strong, dedicated, and talented team of professionals for the manufacturing of many natural and modified nucleoside bases. Included in these natural and modified bases are DMTr protected deoxy and ribo nucleosides, 2’-O-methyl and 2’-O-propargyl nucleosides, and silyl protected ribonucleosides. A large variety of reagents for the synthesis of DNA/RNA are also available. These include 5-thio- ethyltetrazole (ETT), benzylthiotetrazole (BTT/BMT), DMTr-chloride, MMTr-chloride, and several types of phosphorylating reagents such as cyanoethyl chloro phosphitylating reagent, cyanoethyl tetra iso- propyl phosphitylating reagent, cyanoethyl dichloro phosphitylating reagent and 5’, 3’-bis silylating reagent. As the market for oligonucleotides continues to grow, ChemGenes will stay committed to introduc- ing novel products, while maintaining its existing product mix. We also have the capacity to custom synthesize products on request. Our highly trained technical staff is always available and happy to help you with your special needs and demands in this area. For information regarding the full range of products and their applications, please call us at 978-694-4500 or toll-free 800-762-9323, fax: 978-694-4502, or e-mail: [email protected] i Toll Free: (800) 762-9323 33 Industrial Way, Wilmington, MA 01887 • T: (978) 694-4500 • F: (978) 694-4502 Special Gratitude The nucleoside phosphoramidites available in this catalog are the result of the classical research contribution by Professor M.H. Caruthers and colleagues (University of Colorado, Boulder, CO) (Beaucage S. L.; Caruthers, M. H. Tet. Lett., 1981, 22,1859-1862). Other enormous contributions in this field have been made by Professor K.K. Ogilvie and colleagues (Acadia University, Acadia, Canada), Professor R.L. Letsinger and colleagues (Northwestern University, Evanston, IL), and Professor H. G. Khorana, Noble Laureate and colleagues (Massachusetts Institute of Technology, Cambridge, MA). Patents, Licensing & Trademarks ChemGenes; 2’-O-Methyl, Ethyl phosphoramidite, patent # US 6,015,886. ChemGenes; 2’-O-Methyl patent, patent # US 5,525,719. ChemGenes; 3’-O-Methyl patent # US 5,525,719. ChemGenes; 2’-O-Propargyl modified, patent # US 5,744,595. ChemGenes; 3’-O-Propargyl modified Amidites & Solid Supports, patent# US 5,744,595. ChemGenes; 2’-Ara-O-methyl amidites, Patent # US 20120149888. ChemGenes; Reverse RNA synthesis, patent App# WO 2011103468. ChemGenes; Dithiolane based thiol modifier, patent Provisional application # PCT61/79585. ChemGenes; Oligonucleotide Labeling-Cycloaddition Reaction, Patent # US 20120058476. ChemGenes; Modifications of antimetabolite gemcitabine for incorporation in CpG oligonucleotides, Patent # US 20110040082 ChemGenes; N6-Trimethyl-L-Lysine & derivative, their therapeutic uses, Patant # US 20110166230; US 20110166231; US 20110166379; US 20110166380 Qiagen Inc.; TOM Amidites, Patent #5,986,084. UNIVERSITE DE MONTPELLIER II; 2’-O-Pivom amidites, patent #2009144418. NIAIST & DNA Chip Research, Japan; ssR amino linker, patent # 7,491,857 B2. McGill University and Wisconsin Alumni Research Foundation; 2’-O-ALE-5’-NPPOC-amidites patent #025566 A1. Beckman Coulter, Inc., ac-dC chemistry patents# 5,348,868, 5,428,148, 5,518,651, 5,808,039. UnylinkerTM is a Trademark of Isis Pharmaceuticals, Inc. Isis patent #7,202,264. ii www.chemgenes.com Email: [email protected] Tools for Molecular Biology Research Natural DNA Amidites, Supports & Oligo Synthesis Reagents Standard base protection-DNA amidites 3 Standard base protection-DNA supports 4 Mild base protection-DNA amidites 5 Mild base protection-DNA supports 6 Fast deprotection (Using AMA reagent)-DNA amidites 7 Base unprotected-DNA amidites 7 Methyl phosphoramidites 8 Benzyl phosphoramidites 9 Reverse DNA synthesis 10 Supports for reverse DNA synthesis 10 Polystyrene supports for reverse DNA synthesis 11 Levulinyl amidites & levulinyl deprotection solution 12 DNA Dimers 13 DNA H-Phosphonate monomers 14 Succinyl Polystyrene supports 15 Non-cleavable supports 16 Unylinker supports 17 lcaa Amino supports 18 Empty columns, Filters 18 Ancillary Reagents For Automated Oligonucleotide Synthesizer 19-25 (ABI, Akta, Expedite, MerMade Oligonucleotide synthesizers) Modified DNA Amidites and Supports 2-Thio-dU, 2-Thio-T, 4-Thio-T, 4-Thio-dU amidites & supports 27 dI, dU, 5-Methyl-dC amidites & supports 28 α-D-Amidites & supports, 5-Methyl-α-D-dC amidites 29-30 2'-O-TFA-propyl-dU & dC, 3'-TFA-Amino-dC & dU, 3'-Amino-dT, 5'-Amino-dT modifications & Support for 5'-Amino-dT modification 31 Ara nucleoside amidites & supports 32 7-Deaza-dI, 7-Deaza-dA, 7-Deaza-dG amidites & supports 33 7-Deaza-7-iodo-dA & dG amidites, 7-Deaza-7-propargylamino-TFA-dA & dG amidites 34 Cordecypin (3'-dA) amidite & supports, 6-Thio-dG amidite 35 Halogenated nucleoside amidites & supports 36-37 N-Alkylated nucleoside amidites & supports 38-39 O6-Trimethyl-silyl-ethoxy-dI, O6-Diphenylcarbamoyl-dG & Modified purine amidites 40 8-Methyl-dG amidites & supports 41 Modified purine 2’-deoxy riboside amidites & supports 42 Triazolyl-dU & dT-amidites, Iso-dG & dC-amidites, supports for Iso-dC & dG 43 L-DNA (Mirror Image) amidites & supports, 5-Formyl-dU 44-45 Abasic amidite, Reverse abasic amidite & supports, 8-Oxo-dA & dG amidites & supports 46 Dideoxy amidites and supports 47 5'-O-Methyl DNA amidites, DNA 3',5'-Diamidites & Thymidine 3'-Bis (diisopropylamino)-phosphoramidite 48 DNA purification kit 49 DNA purification drying traps 50 DNA purification cartridges and barrels 52 iii Tools for Molecular Biology Research Natural RNA Amidites and Supports Standard base protection-RNA amidites 55 Supports for RNA synthesis (Standard base protection) 56 Labile base protection-RNA amidites 57 Supports for RNA synthesis (Mild base protection) 58 Oxalyl RNA supports and Ammonia free oligo deprotection solution 59 2'-O-ALE amidites 60 2'-O-Pivom amidites 61 3'-Polystyrene supports for RNA synthesis 62 RNA methyl phosphoramidites 63 Labile
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