KERAPU TELUK KWANDANG (Groupers of Kwandang Bay)
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RNA Detection Technology for Applications in Marine Science: Microbes to Fish Robert Michael Ulrich University of South Florida, [email protected]
University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School 6-25-2014 RNA Detection Technology for Applications in Marine Science: Microbes to Fish Robert Michael Ulrich University of South Florida, [email protected] Follow this and additional works at: https://scholarcommons.usf.edu/etd Part of the Biology Commons, and the Molecular Biology Commons Scholar Commons Citation Ulrich, Robert Michael, "RNA Detection Technology for Applications in Marine Science: Microbes to Fish" (2014). Graduate Theses and Dissertations. https://scholarcommons.usf.edu/etd/5321 This Dissertation is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. RNA Detection Technology for Applications in Marine Science: Microbes to Fish by Robert M. Ulrich A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy College of Marine Science University of South Florida Major Professor: John H. Paul, Ph.D. Valerie J. Harwood, Ph.D. Mya Breitbart, Ph.D. Christopher D. Stallings, Ph.D. David E. John, Ph.D. Date of Approval June 25, 2014 Keywords: NASBA, grouper, Karenia mikimotoi, Enterococcus Copyright © 2014, Robert M. Ulrich DEDICATION This dissertation is dedicated to my fiancée, Dr. Shannon McQuaig for inspiring my return to graduate school and her continued support over the last four years. On no other porch in our little town have there been more impactful scientific discussions, nor more words of encouragement. ACKNOWLEDGMENTS I gratefully acknowledge the many people who have encouraged and advised me throughout my graduate studies. -
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e · ~ e t · aI ' A Field Guide to Grouper and Snapper Fishes of Andaman and Nicobar Islands (Family: SERRANIDAE, Subfamily: EPINEPHELINAE and Family: LUTJANIDAE) P. T. RAJAN Andaman & Nicobar Regional Station Zoological Survey of India Haddo, Port Blair - 744102 Edited by the Director, Zoological Survey of India, Kolkata Zoological Survey of India Kolkata CITATION Rajan, P. T. 2001. Afield guide to Grouper and Snapper Fishes of Andaman and Nicobar Islands. (Published - Director, Z.5.1.) Published : December, 2001 ISBN 81-85874-40-9 Front cover: Roving Coral Grouper (Plectropomus pessuliferus) Back cover : A School of Blue banded Snapper (Lutjanus lcasmira) © Government of India, 2001 ALL RIGHTS RESERVED • No part of this publication may be reproduced, stored in a retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise without the prior permission of the publisher. • This book is sold subject to the condition that it shall not, by way of trade, be lent, re-sold, hired out or otherwise disposed of without the publisher'S consent, in any form of binding or cover other than that in which it is published. • The correct price of this publication is the price printed on this page. Any revised price indicated by a rubber stamp or by a sticker or by any other means is incorrect and should be unacceptable. PRICE Indian Rs. 400.00 Foreign $ 25; £ 20 Published at the Publication Division by the Director, Zoological Survey of India, 234/4, AJe Bose Road, 2nd MSO Building, (13th Floor), Nizam Palace, Calcutta-700 020 after laser typesetting by Computech Graphics, Calcutta 700019 and printed at Power Printers, New Delhi - 110002. -
Academic Paper on “Restricting the Size of Groupers (Serranidae
ACADEMIC PAPER ON “RESTRICTING THE SIZE OF GROUPERS (SERRANIDAE) EXPORTED FROM INDONESIA IN THE LIVE REEF FOOD FISH TRADE” Coastal and Marine Resources Management in the Coral Triangle-Southeast Asia (TA 7813-REG) Tehcnical Report ACADEMIC PAPER ON RESTRICTING THE SIZE OFLIVE GROUPERS FOR EXPORT ACADEMIC PAPER ON “RESTRICTING THE SIZE OF GROUPERS (SERRANIDAE) EXPORTED FROM INDONESIA IN THE LIVE REEF FOOD FISH TRADE” FINAL VERSION COASTAL AND MARINE RESOURCES MANAGEMENT IN THE CORAL TRIANGLE: SOUTHEAST ASIA, INDONESIA, MALAYSIA, PHILIPPINES (TA 7813-REG) ACADEMIC PAPER ON RESTRICTING THE SIZE OFLIVE GROUPERS FOR EXPORT Page i FOREWORD Indonesia is the largest exporter of live groupers for the live reef fish food trade. This fisheries sub-sector plays an important role in the livelihoods of fishing communities, especially those living on small islands. As a member of the Coral Triangle Initiative (CTI), in partnership with the Asian Development Bank (ADB) under RETA [7813], Indonesia (represented by a team from Hasanuddin University) has compiled this academic paper as a contribution towards sustainable management of live reef fish resources in Indonesia. Challenges faced in managing the live grouper fishery and trade in Indonesia include the ongoing activities and practices which damage grouper habitat; the lack of protection for grouper spawning sites; overfishing of groupers which have not yet reached sexual maturity/not reproduced; and the prevalence of illegal and unreported fishing for live groupers. These factors have resulted in declining wild grouper stocks. The Aquaculture sector is, at least as yet, unable to replace or enable a balanced wild caught fishery, and thus there is still a heavy reliance on wild-caught groupers. -
PP-4. Monitoring of Fish Supply to Resorts and Setting up of an Ecolabel Certification
PP-4. Monitoring of Fish Supply to Resorts and Setting up of an Ecolabel Certification 1) Report on Survey on Reef Fish Landings to Tourist Resorts 2) Guidelines on Best Fishing and Fish Handling Practices 3) Overview of reef fish sampling in K. Dhiffushi – Nov-Dec 2016 REPORT ON SURVEY ON REEF FISH LANDINGS TO TOURIST RESORTS May 2016 Muawin YOOSUF, Ministry of Fisheries and Agriculture with the technical assistance of Bernard ADRIEN, MASPLAN This survey was carried out as part of a Pilot Project under the Project for the Formulation of Master Plan for Sustainable Fisheries (MASPLAN), a technical cooperation project of the Japan International Cooperation Agency (JICA). All pictures taken by Bernard Adrien. REPORT ON SURVEY ON REEF FISH LANDINGS TO RESORTS – MAY 2016 1 Table of Contents 1 INTRODUCTION .................................................................................................................................3 2 METHOD ..............................................................................................................................................4 3 RESULTS & ANALYSIS .....................................................................................................................5 3.1 Estimates on reef fish production ..................................................................................................5 Estimate of Annual Reef Fish Landings to Resorts from the present survey ................................5 Comparison on Annual Reef Fish Landings to Resorts with previous surveys ............................5 -
Snapper and Grouper: SFP Fisheries Sustainability Overview 2015
Snapper and Grouper: SFP Fisheries Sustainability Overview 2015 Snapper and Grouper: SFP Fisheries Sustainability Overview 2015 Snapper and Grouper: SFP Fisheries Sustainability Overview 2015 Patrícia Amorim | Fishery Analyst, Systems Division | [email protected] Megan Westmeyer | Fishery Analyst, Strategy Communications and Analyze Division | [email protected] CITATION Amorim, P. and M. Westmeyer. 2016. Snapper and Grouper: SFP Fisheries Sustainability Overview 2015. Sustainable Fisheries Partnership Foundation. 18 pp. Available from www.fishsource.com. PHOTO CREDITS left: Image courtesy of Pedro Veiga (Pedro Veiga Photography) right: Image courtesy of Pedro Veiga (Pedro Veiga Photography) © Sustainable Fisheries Partnership February 2016 KEYWORDS Developing countries, FAO, fisheries, grouper, improvements, seafood sector, small-scale fisheries, snapper, sustainability www.sustainablefish.org i Snapper and Grouper: SFP Fisheries Sustainability Overview 2015 EXECUTIVE SUMMARY The goal of this report is to provide a brief overview of the current status and trends of the snapper and grouper seafood sector, as well as to identify the main gaps of knowledge and highlight areas where improvements are critical to ensure long-term sustainability. Snapper and grouper are important fishery resources with great commercial value for exporters to major international markets. The fisheries also support the livelihoods and food security of many local, small-scale fishing communities worldwide. It is therefore all the more critical that management of these fisheries improves, thus ensuring this important resource will remain available to provide both food and income. Landings of snapper and grouper have been steadily increasing: in the 1950s, total landings were about 50,000 tonnes, but they had grown to more than 612,000 tonnes by 2013. -
BIOT Field Report
©2015 Khaled bin Sultan Living Oceans Foundation. All Rights Reserved. Science Without Borders®. All research was completed under: British Indian Ocean Territory, The immigration Ordinance 2006, Permit for Visit. Dated 10th April, 2015, issued by Tom Moody, Administrator. This report was developed as one component of the Global Reef Expedition: BIOT research project. Citation: Global Reef Expedition: British Indian Ocean Territory. Field Report 19. Bruckner, A.W. (2015). Khaled bin Sultan Living Oceans Foundation, Annapolis, MD. pp 36. The Khaled bin Sultan Living Oceans Foundation (KSLOF) was incorporated in California as a 501(c)(3), public benefit, Private Operating Foundation in September 2000. The Living Oceans Foundation is dedicated to providing science-based solutions to protect and restore ocean health. For more information, visit http://www.lof.org and https://www.facebook.com/livingoceansfoundation Twitter: https://twitter.com/LivingOceansFdn Khaled bin Sultan Living Oceans Foundation 130 Severn Avenue Annapolis, MD, 21403, USA [email protected] Executive Director Philip G. Renaud Chief Scientist Andrew W. Bruckner, Ph.D. Images by Andrew Bruckner, unless noted. Maps completed by Alex Dempsey, Jeremy Kerr and Steve Saul Fish observations compiled by Georgia Coward and Badi Samaniego Front cover: Eagle Island. Photo by Ken Marks. Back cover: A shallow reef off Salomon Atoll. The reef is carpeted in leather corals and a bleached anemone, Heteractis magnifica, is visible in the fore ground. A school of giant trevally, Caranx ignobilis, pass over the reef. Photo by Phil Renaud. Executive Summary Between 7 March 2015 and 3 May 2015, the Khaled bin Sultan Living Oceans Foundation conducted two coral reef research missions as components of our Global Reef Expedition (GRE) program. -
Fish, Crustaceans, Molluscs, Etc Capture Production by Species Items Indian Ocean, Western C-51 Poissons, Crustacés, Mollusques
481 Fish, crustaceans, molluscs, etc Capture production by species items Indian Ocean, Western C-51 Poissons, crustacés, mollusques, etc Captures par catégories d'espèces Océan Indien, ouest (a) Peces, crustáceos, moluscos, etc Capturas por categorías de especies Océano Índico, occidental English name Scientific name Species group Nom anglais Nom scientifique Groupe d'espèces 2003 2004 2005 2006 2007 2008 2009 Nombre inglés Nombre científico Grupo de especies t t t t t t t Kelee shad Hilsa kelee 24 2 306 3 534 3 513 4 151 5 227 3 765 4 616 Hilsa shad Tenualosa ilisha 24 8 257 4 024 6 123 3 233 7 345 5 305 5 617 Bloch's gizzard shad Nematalosa nasus 24 ... 93 15 71 196 263 216 Milkfish Chanos chanos 25 113 101 122 159 135 156 322 Barramundi(=Giant seaperch) Lates calcarifer 25 - - - 0 0 0 - Leopard flounder Bothus pantherinus 31 96 70 91 124 91 93 95 Lefteye flounders nei Bothidae 31 73 78 20 85 1 1 15 Mud sole Austroglossus pectoralis 31 - - - - - 19 - Tonguefishes Cynoglossidae 31 1 383 984 907 907 909 1 002 1 011 Indian halibut Psettodes erumei 31 2 365 2 943 3 792 4 191 5 050 4 975 3 808 Flatfishes nei Pleuronectiformes 31 15 924 13 630 13 582 14 658 22 782 17 697 21 709 Unicorn cod Bregmaceros mcclellandi 32 2 643 1 322 2 717 1 597 852 674 636 Cape hakes Merluccius capensis,M.paradox. 32 1 3 5 5 3 3 3 Gadiformes nei Gadiformes 32 - - - - - 55 - Bombay-duck Harpadon nehereus 33 101 936 135 340 158 829 152 838 185 088 218 639 178 003 Greater lizardfish Saurida tumbil 33 3 634 2 655 4 167 3 656 2 575 2 564 3 030 Brushtooth lizardfish -
First Report of Philometra Sp. Infestation in Yellow Edged Lyretail Grouper Variola Louti
International Journal of Fisheries and Aquatic Studies 2018; 6(6): 33-36 E-ISSN: 2347-5129 P-ISSN: 2394-0506 (ICV-Poland) Impact Value: 5.62 First report of Philometra sp. infestation in Yellow (GIF) Impact Factor: 0.549 IJFAS 2018; 6(6): 33-36 edged lyretail grouper Variola louti (Forsskål, 1775) © 2018 IJFAS www.fisheriesjournal.com from Indian waters Received: 14-09-2018 Accepted: 18-10-2018 Rekha J Nair, Archana C, NK Sanil, PK Seetha, M Radhakrishnan, Sunil Rekha J Nair Demersal Fisheries Division, KTS and Akhildev S ICAR-Central Marine Fisheries Research Institute (ICAR- Abstract CMFRI), Kochi, Kerala, India Studies were carried out to assess the prevalence of nematode infestation in the gonads of the yellow edged lyretail grouper, Variola louti, over a two year period. The results showed the presence of Archana C nematodes during post monsoon months. Incidences of gonad atrophy were also noted leading to Marine Biotechnology Division, isolation and identification of the nematode. The Yellow edged lyretail a species which contributes to the ICAR-Central Marine Fisheries hooks and line fishery, was seen to be infected with nematodes from the Philometra genera. Since the Research Institute (ICAR- CMFRI), Kochi, Kerala, India nematode is very specific to each fish further studies are essential for its identification. NK Sanil Keywords: Philometra, nematode, Variola, grouper, parasitic infestation Marine Biotechnology Division, ICAR-Central Marine Fisheries Introduction Research Institute (ICAR- Variola louti, commonly called the Yellow edged lyretail grouper, is a commercially important CMFRI), Kochi, Kerala, India species contributing to the hook and line fishery in Kerala, on the south west coast of India PK Seetha (Fig.1). -
Training Manual Series No.15/2018
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by CMFRI Digital Repository DBTR-H D Indian Council of Agricultural Research Ministry of Science and Technology Central Marine Fisheries Research Institute Department of Biotechnology CMFRI Training Manual Series No.15/2018 Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18 Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18 Training Manual This is a limited edition of the CMFRI Training Manual provided to participants of the “DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals” organized by the Marine Biotechnology Division of Central Marine Fisheries Research Institute (CMFRI), from 2nd February 2015 - 31st March 2018. Principal Investigator Dr. P. Vijayagopal Compiled & Edited by Dr. P. Vijayagopal Dr. Reynold Peter Assisted by Aditya Prabhakar Swetha Dhamodharan P V ISBN 978-93-82263-24-1 CMFRI Training Manual Series No.15/2018 Published by Dr A Gopalakrishnan Director, Central Marine Fisheries Research Institute (ICAR-CMFRI) Central Marine Fisheries Research Institute PB.No:1603, Ernakulam North P.O, Kochi-682018, India. 2 Foreword Central Marine Fisheries Research Institute (CMFRI), Kochi along with CIFE, Mumbai and CIFA, Bhubaneswar within the Indian Council of Agricultural Research (ICAR) and Department of Biotechnology of Government of India organized a series of training programs entitled “DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals”. -
National Prioritization of Key Vulnerable Reef Fish Species for Fiji, for Targeted Research
National prioritization of key vulnerable reef fish species for Fiji, for targeted research Coral reef fish and invertebrates sold at the Suva market. Photo by: Sangeeta Mangubhai/WCS Introduction The majority of Fiji’s population is coastal and therefore highly reliant on inshore fisheries for their subsistence and local economic needs (Hunt 1999). At least 33 percent of all animal protein consumed in Fiji comes from fish, and subsistence and artisanal fisheries contribute at least US$59.1 million to Fiji’s annual GDP (Gillett 2009). There is growing concerns for the impacts of present day harvesting rates and methods, especially for vulnerable fish and invertebrate species in Fiji. This is resulting in a progressive decline in fish belonging to higher trophic (feeding) groups, a pattern that is termed “fishing down food webs” (Pauly et al. 1998). Coral reef fish vary in their vulnerability to fishing pressure, and how well they can recover, if fishing is stopped or significantly reduced. Recovery potential relates to the rate at which a species can replace the individuals that are lost to natural mortality and to fishing. In general, the medium to larger carnivorous fish high in the food chain are thought to be more vulnerable to fishing (e.g. groupers) requiring in decades to recover, while smaller fish (e.g. herbivores such as rabbitfish) are thought be less vulnerable (Abesamis et al. 2014). Certain life history characteristics of fish species together can be good predictors of vulnerability at the population level to fishing pressure, including: (a) maximum size; (b) body growth rate; (c) lifespan; (d) natural mortality rates; (e) age at maturity; and (f) length at maturity (Abesamis et al. -
Ensuring Seafood Identity: Grouper Identification by Real-Time Nucleic
Food Control 31 (2013) 337e344 Contents lists available at SciVerse ScienceDirect Food Control journal homepage: www.elsevier.com/locate/foodcont Ensuring seafood identity: Grouper identification by real-time nucleic acid sequence-based amplification (RT-NASBA) Robert M. Ulrich a, David E. John b, Geran W. Barton c, Gary S. Hendrick c, David P. Fries c, John H. Paul a,* a College of Marine Science, MSL 119, University of South Florida, 140 Seventh Ave. South, St. Petersburg, FL 33701, USA b Department of Biological Sciences, University of South Florida St. Petersburg, 140 Seventh Ave. S., St. Petersburg, FL 33701, USA c EcoSystems Technology Group, College of Marine Science, University of South Florida, 140 Seventh Ave. S., St. Petersburg, FL 33701, USA article info abstract Article history: Grouper are one of the most economically important seafood products in the state of Florida and their Received 19 September 2012 popularity as a high-end restaurant dish is increasing across the U.S. There is an increased incidence rate Accepted 3 November 2012 of the purposeful, fraudulent mislabeling of less costly and more readily available fish species as grouper in the U.S., particularly in Florida. This is compounded by commercial quotas on grouper becoming Keywords: increasingly more restrictive, which continues to drive both wholesale and restaurant prices higher each RT-NASBA year. Currently, the U.S. Food and Drug Administration recognize 56 species of fish that can use “grouper” FDA seafood list as an acceptable market name for interstate commerce. This group of fish includes species from ten Grouper fi fi Mislabeling different genera, making accurate taxonomic identi cation dif cult especially if distinguishing features such as skin, head, and tail have been removed. -
Assessing the Vulnerability of Marine Life to Climate Change in the Pacific Region
Assessing the vulnerability of marine life to climate change in the Pacific Region Jonatha Giddens1, Donald Kobayashi1, Mark Nelson2 1.NOAA Fisheries Pacific Islands Fisheries Science Center 2.NOAA Fisheries Office of Science and Technology Outline • Project overview • VA general methodology • Progress • Pacific Islands preliminary results • Next steps Which species are most at risk? Cartoon drawn by Bas Kohler at ECCWO 2018 Scale of assessment methods adapted from Holsman et al. 2017 Where are the assessments being done? Morrison et al. 2015; Hare et al. 2016 Pacific Islands Vulnerability Assessment 6 Methodology framework Stock Vulnerability Exposure Sensitivity • Temperature (air, bottom, SS) Habitat specificity • Salinity (surface, bottom) • • Complexity in reproduction • Ocean acidification (pH) • Prey specificity • Early life history survival & • Mixed layer depth • Sensitivity to OA • Precipitation • Sensitivity to temperature settlement requirements • Currents (NS, EW) • Population growth rate • Windstress (Mag, NS, EW) • Stock size/status • Surface oxygen • Other stressors • Dispersal of early life stages • Sea level rise • Adult mobility • Chlorophyll, productivity • Spawning cycle Taxonomic scope 83 species 33 families 6 functional groups Methodology overview: sensitivity • T rait-based • E xisting knowledge + expert opinion • Group workshop to discuss results Methodology overview: exposure Surface oxygen – hammerhead shark • Measure of how much a species is likely to experience a change in climate • Spatial overlap - species’