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Digestive Glands A560 – Fall 2015 Digestive Glands

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Digestive Glands A560 – Fall 2015 Digestive Glands Lab 16 –Digestive Glands A560 – Fall 2015 Digestive Glands I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder IV. Summary Fig 15‐1, Junqueira, 13th ed. Lab 16 –Digestive Glands A560 – Fall 2015 Learning Objectives I. Introduction II. Learning Objectives III. Slides and Micrographs 1. Review the secretory units of the salivary glands. A. Salivary Glands 1. Parotid gland 2. Understand the structure and function of the cuboidal epithelium cells 2. Submandibular gland comprising the ducts. B. Pancreas C. Liver 3. Compare and contrast the histology and exocrine function of the pancreas with D. Gall Bladder IV. Summary that of the parotid salivary gland. 4. Understand the overall organization of lobes and vasculature in the liver. 5. Understand the microvascular features specific to the liver and the function of the hepatocytes. 6. Understand the origin of bile and the system transporting it from the liver. 7. Understand the structure of the gall bladder wall and how it facilitates the organ’s function. Lab 16 –Digestive Glands A560 – Fall 2015 Comparison of Major Salivary Glands I. Introduction II. Learning Objectives III. Slides and Micrographs Slide 132: Parotid Gland, H&E Slide 11: Submandibular Gland, PAS & AB A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder IV. Summary • Compound acinar • Compound tubuloacinar • Serous acini only • Serous acini and mucuous tubules • Serous demilunes Lab 16 –Digestive Glands A560 – Fall 2015 Excretory Pathway from Salivary Glands I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands Secretory cells 1. Parotid gland 2. Submandibular gland B. Pancreas Intercalated ducts: small/closed‐appearing lumen (generally smaller than acinus); lined C. Liver with simple squamous or cuboidal epithelium; often surrounded by myoepithelial cells D. Gall Bladder Intralobular IV. Summary Striated (intralobular) ducts: larger, open lumen; lined with simple cuboidal (or columnar) cells with basal striations Interlobular ducts: in septa; increasing size; and epithelium transitioning from simple cuboidal to columnar epithelium; may be stratified Excretory (lobar) ducts: largest; from superficial and deep lobes of gland; stratified “Excretory” cuboidal or maybe columnar epithelium Lab 16 –Digestive Glands A560 – Fall 2015 Slide 132: Parotid Gland, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands capsule 1. Parotid gland 2. Submandibular gland B. Pancreas septa divide C. Liver lobules of glands D. Gall Bladder IV. Summary excretory duct lobule striated ducts presence of ducts distinguishes organ as exocrine gland – so not to be confused with any of the lymphoid structures Lab 16 –Digestive Glands A560 – Fall 2015 I. Introduction Slide 132: Parotid Gland, H&E II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder septum IV. Summary striated ducts adipose excretory duct Lab 16 –Digestive Glands A560 – Fall 2015 Slide 132: Parotid Gland, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland striated duct 2. Submandibular gland parenchyma B. Pancreas is composed C. Liver of serous D. Gall Bladder acini only IV. Summary adipose excretory duct in septum Lab 16 –Digestive Glands A560 – Fall 2015 Slide 132: Parotid Gland, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland striated duct with faint B. Pancreas “striation”’in basal half of C. Liver cell perpendicular to D. Gall Bladder lumen IV. Summary Lab 16 –Digestive Glands A560 – Fall 2015 Slide 11: Submandibular Gland, PAS AB I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands parenchyma consists 1. Parotid gland of both serous and 2. Submandibular gland mucous cells B. Pancreas C. Liver D. Gall Bladder IV. Summary Dark staining cells = mucous cells Light staining cells = serous cells striated duct Lab 16 –Digestive Glands A560 – Fall 2015 Slide 11: Submandibular Gland, PAS AB I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder IV. Summary Notice the difference in tissue lining lumen between duct and vessel excretory duct Lab 16 –Digestive Glands A560 – Fall 2015 Slide 72: Submandibular Gland, H&Phloxine I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands Mucous cells 1. Parotid gland 2. Submandibular gland Mucous = Adj. B. Pancreas Mucus = Noun C. Liver D. Gall Bladder IV. Summary Serous cells Serous demilune: “half moon” shape of serous portion of striated gland surrounding mucous portion ducts Serous acini secrete non‐glycosylated protein products and stain strongly due to their abundant rER and secretory granules; Mucous acini secrete mucus (mucopolysaccharide) and contain little rER so tend to be poorer‐staining with most general stains Lab 16 –Digestive Glands A560 – Fall 2015 Slide 15: Pancreas, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland like the salivary glands, B. Pancreas notice organization into of C. Liver pancreas (compound acinar) D. Gall Bladder into lobules, separated by IV. Summary CT septa; however, notice the lack of conspicuous striated (intralobular) ducts in the lobules as were seen in the salivary glands Lab 16 –Digestive Glands A560 – Fall 2015 I. Introduction Slide 154: Pancreas, H&E II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland pancreas is divided into 2. Submandibular gland excretory and endocrine B. Pancreas portions: C. Liver D. Gall Bladder Dark cells = IV. Summary secretory acini (excretory) Light clusters of cells = Pancreatic islets (endocrine) (Islets of Langerhans) Lab 16 –Digestive Glands A560 – Fall 2015 Slide 154: Pancreas, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs intralobular excretory duct A. Salivary Glands (only one type of intralobular 1. Parotid gland duct, unlike in salivary glands) 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder IV. Summary Secretory acini (exocrine) pancreatic islet or islet of Langerhans (endocrine) Lab 16 –Digestive Glands A560 – Fall 2015 Slide 154: Pancreas, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas secretory C. Liver acinus D. Gall Bladder IV. Summary serous acini of the exocrine pancreas have 5‐10 cells facing central lumen;apicalendsare eosinophilic due to secretory granules; basal ends are basophilic due to nucleus and rER Lab 16 –Digestive Glands A560 – Fall 2015 I. Introduction Slide 24: Liver & GB, Masson Trichrome II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder larger spaces = portal triads IV. Summary smaller spaces = central veins Lab 16 –Digestive Glands A560 – Fall 2015 I. Introduction Slide 141: Liver, H&E II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland portal B. Pancreas triad C. Liver D. Gall Bladder hepatic IV. Summary artery bile duct Lab 16 –Digestive Glands A560 – Fall 2015 I. Introduction Slide 141: Liver, H&E II. Learning Objectives III. Slides and Micrographs A. Salivary Glands Portal Tract/Triad 1. Parotid gland hepatic portal vein 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder IV. Summary hepatic artery bile duct Lab 16 –Digestive Glands A560 – Fall 2015 Slide 24: Liver, Masson Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder hepatic IV. Summary lobule central vein Lab 16 –Digestive Glands A560 – Fall 2015 Slide 29: Liver, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland central vein B. Pancreas C. Liver D. Gall Bladder portal triad IV. Summary Lab 16 –Digestive Glands A560 – Fall 2015 Slide 24: Liver, Masson Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs Kupffer cell A. Salivary Glands (macrophage) 1. Parotid gland in sinusoid 2. Submandibular gland B. Pancreas C. Liver hepatocytes D. Gall Bladder IV. Summary hepatic sinusoids lined by endothelial cells Lab 16 –Digestive Glands A560 – Fall 2015 Slide 24: Liver, Masson Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder lipofuscin IV. Summary Lab 16 –Digestive Glands A560 – Fall 2015 Slide 94: Gallbladder, Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. Salivary Glands 1. Parotid gland 2. Submandibular gland B. Pancreas C. Liver D. Gall Bladder IV. Summary serosa on free surface; covered by adventitia where bound by liver muscularis lamina propria lumen mucosa note the lack of muscularis mucosae and submucosa; the lamina propria is directly on the muscularis layer Lab 16 –Digestive Glands A560 – Fall 2015 I. Introduction Slide 94: Gallbladder, Trichrome II. Learning
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