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Quantigene Flowrna Probe Sets Currently Available

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QuantiGene FlowRNA Probe Sets Currently Available Accession No. Species Symbol Gene Name Catalog No. NM_003452 Human ZNF189 zinc finger protein 189 VA1-10009 NM_000057 Human BLM Bloom syndrome VA1-10010 NM_005269 Human GLI glioma-associated oncogene homolog (zinc finger protein) VA1-10011 NM_002614 Human PDZK1 PDZ domain containing 1 VA1-10015 NM_003225 Human TFF1 Trefoil factor 1 (breast cancer, estrogen-inducible sequence expressed in) VA1-10016 NM_002276 Human KRT19 keratin 19 VA1-10022 NM_002659 Human PLAUR plasminogen activator, urokinase receptor VA1-10025 NM_017669 Human ERCC6L excision repair cross-complementing rodent repair deficiency, complementation group 6-like VA1-10029 NM_017699 Human SIDT1 SID1 transmembrane family, member 1 VA1-10032 NM_000077 Human CDKN2A cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) VA1-10040 NM_003150 Human STAT3 signal transducer and activator of transcripton 3 (acute-phase response factor) VA1-10046 NM_004707 Human ATG12 ATG12 autophagy related 12 homolog (S. cerevisiae) VA1-10047 NM_000737 Human CGB chorionic gonadotropin, beta polypeptide VA1-10048 NM_001017420 Human ESCO2 establishment of cohesion 1 homolog 2 (S. cerevisiae) VA1-10050 NM_197978 Human HEMGN hemogen VA1-10051 NM_001738 Human CA1 Carbonic anhydrase I VA1-10052 NM_000184 Human HBG2 Hemoglobin, gamma G VA1-10053 NM_005330 Human HBE1 Hemoglobin, epsilon 1 VA1-10054 NR_003367 Human PVT1 Pvt1 oncogene homolog (mouse) VA1-10061 NM_000454 Human SOD1 Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) VA1-10069 NM_000088 Human COL1A1 collagen, type 1, alpha 1 VA1-10070 NM_003358 Human UGCG UDP-glucose ceramide glucosyltransferase VA1-10073 NM_005574 Human LMO2 LIM domain only 2 (rhombotin-like 1) VA1-10074 XM_084664 Human LOC143916 similar to hypothetical protein FLJ21617; erythroid differentiation-related factor 1 VA1-10075 NM_005239 Human ETS2 v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) VA1-10076 NM_000067 Human CA2 carbonic anhydrase II VA1-10078 NM_002127 Human HLA-G major histocompatibility complex, class I, G VA1-10079 NM_000559 Human HBG1 hemoglobin, gamma A VA1-10080 NM_000640 Human IL13RA2 Interleukin 13 receptor, alpha 2 VA1-10085 NM_005157 Human ABL1 v-abl Abelson murine leukemia viral oncogene homolog 1 VA1-10096 NM_000207 Human INS Insulin VA1-10099 NM_004530 Human MMP2 matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) VA1-10113 NM_002421 Human MMP1 Matrix metalloproteinase 1 (interstitial collagenase) VA1-10114 NM_002046 Human GAPD glyceraldehyde-3-phosphate dehydrogenase VA1-10119 NM_052872 Human IL17F interleukin 17F VA1-10122 NM_002190 Human IL17 Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) VA1-10123 NM_002133 Human HMOX1 heme oxygenase (decycling) 1 VA1-10124 NM_004083 Human DDIT3 DNA-damage-inducible transcript 3 VA1-10126 NM_006763 Human BTG2 BTG family, member 2 VA1-10128 NM_002306 Human LGALS3 lectin, galactoside-binding, soluble, 3 VA1-10130 NM_000942 Human PPIB peptidylprolyl isomerase B (cyclophilin B) VA1-10148 NM_005556 Human KRT7 Keratin 7 VA1-10151 NM_005037 Human PPARG peroxisome proliferative activated receptor, gamma VA1-10156 NM_001718 Human BMP6 Bone morphogenetic protein 6 VA1-10159 NM_005502 Human ABCA1 ATP-binding cassette, sub-family A (ABC1), member 1 VA1-10160 NM_001524 Human HCRT hypocretin (orexin) neuropeptide precursor VA1-10167 NM_000598 Human IGFBP3 insuline-like growth factor binding protein 3 VA1-10168 NM_000189 Human HK2 hexokinase 2 VA1-10169 NM_005080 Human XBP1 X-box binding protein 1 VA1-10174 NM_005045 Human RELN Reelin VA1-10179 NM_170731 Human BDNF brain-derived neurotrophic factor VA1-10184 NM_001530 Human HIF1A hypoxia-inducible factor 1, alpha subuinit VA1-10185 X03205 Human 18S 18S ribosomal VA1-10188 NM_000633 Human BCL2 B-cell CLL/lymphoma 2 VA1-10189 NM_017460 Human CYP3A4 cytochrome P450, family 3, subfamily A, polypeptide 4 VA1-10196 NM_000384 Human APOB apolipoprotein B (including Ag(x) antigen) VA1-10197 NM_000118 Human ENG Endoglin (Osler-Rendu-Weber syndrome 1) VA1-10201 NM_021009 Human UBC Ubiquitin C VA1-10203 NM_001753 Human CAV1 caveolin 1, caveolae protein, 22kDa VA1-10205 NM_020396 Human BCL2L10 BCL2-like 10 (apoptosis facilitator) VA1-10206 NM_001419 Human ELAVL1 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) VA1-10214 NM_000680 Human ADRA1A adrenergic, alpha-1A-, receptor VA1-10215 NM_152860 Human SP7 Sp7 transcription factor VA1-10217 NM_004523 Human KIF11 kinesin family member 11 VA1-10219 NM_019113 Human FGF21 fibroblast growth factor 21 VA1-10220 QuantiGene FlowRNA Probe Sets Currently Available Accession No. Species Symbol Gene Name Catalog No. NM_012111 Human AHSA1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) VA1-10243 NM_002055 Human GFAP Glial fibrillary acidic protein VA1-10253 NM_003467 Human CXCR4 chemokine (C-X-C motif) receptor 4 VA1-10263 NM_016639 Human TNFRSF12A tumor necrosis factor receptor superfamily, member 12A VA1-10265 NM_016270 Human KLF2 Kruppel-like factor 2 (lung) VA1-10266 NM_033035 Human TSLP Thymic stromal lymphopoietin VA1-10267 NM_015717 Human CD207 CD207 antigen, langerin VA1-10268 NM_014398 Human LAMP3 Lysosomal-associated membrane protein 3 VA1-10269 NM_005191 Human CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) VA1-10270 AF103907 Human DD3-PCA3 non-coding RNA DD3 VA1-10271 NM_000758 Human CSF2 colony stimulating factor 2 (granulocyte-macrophage) VA1-10275 NM_001613 Human ACTA2 actin, alpha, smooth muscle, aorta VA1-10300 NM_001048 Human SST somatostatin VA1-10301 NM_002062 Human GLP1R Glucagon-like peptide 1 receptor VA1-10302 NM_001742 Human CALCR Calcitonin receptor VA1-10303 NM_001741 Human CALCA Calcitonin-related polypeptide alpha VA1-10304 NM_000632 Human ITGAM Integrin, alpha M VA1-10313 NM_174936 Human PCSK9 Proprotein convertase subtilisin/kexin type 9 VA1-10322 NM_030906 Human STK33 serine/threonine kinase 33 VA1-10324 NM_004456 Human EZH2 Enhancer of zeste homolog 2 (Drosophila) VA1-10325 NM_004958 Human FRAP1 FK506 binding protein 12-rapamycin associated protein 1 VA1-10326 NM_002467 Human MYC v-myc myelocytomatosis viral oncogene homolog (avian) VA1-10327 NM_016204 Human GDF2 growth differentiation factor 2 VA1-10343 NM_018685 Human ANLN anillin, actin binding protein VA1-10344 NM_015341 Human BRRN1 Barren homolog (Drosophila) VA1-10345 NM_007019 Human UBE2C Ubiquitin-conjugating enzyme E2C VA1-10346 NM_006845 Human KIF2C Kinesin family member 2C VA1-10347 NM_001611 Human ACP5 Acid phosphatase 5, tartrate resistant VA1-10348 NM_000039 Human APOA1 apolipoprotein A-1 VA1-10349 NM_001101 Human ACTB actin, beta VA1-10351 NM_182762 Human MACC1 Metastasis associated in colon cancer 1 VA1-10353 NM_003236 Human TGFA transforming growth factor, alpha VA1-10357 NM_001945 Human DTR Diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) VA1-10358 NM_000963 Human PTGS2 prosaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cylooxygenase) VA1-10359 NM_000610 Human CD44 CD44 antigen (homing function and Indian blood group system) VA1-10360 NM_000582 Human SPP1 secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) VA1-10362 NM_000245 Human MET met proto-oncogene (hepatocyte growth factor receptor) VA1-10363 NM_001526 Human HCRTR2 hypocretin (orexin) receptor 2 VA1-10364 NM_007126 Human VCP valosin-containing protein VA1-10366 NM_003318 Human TTK TTK protein kinase VA1-10367 NM_016320 Human NUP98 nucleoporin 98kDa VA1-10368 NM_031966 Human CCNB1 cyclin B1 VA1-10369 NM_000424 Human KRT5 Keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) VA1-10373 NM_015973 Human GAL galanin prepropeptide VA1-10374 NM_024551 Human ADIPOR2 Adiponectin receptor 2 VA1-10376 NM_015999 Human ADIPOR1 Adiponectin receptor 1 VA1-10377 NM_001648 Human KLK3 Kallikrein-related peptidase 3 VA1-10381 NM_004655 Human AXIN2 axin 2 (conductin, axil) VA1-10388 NM_002609 Human PDGFRB Platelet-derived growth factor receptor, beta polypeptide VA1-10395 NM_003873 Human NRP1 neuropilin 1 VA1-10396 NM_006017 Human PROM1 Prominin 1 VA1-10400 NM_001010 Human RPS6 Ribosomal protein S6 VA1-10417 NM_001402 Human EEF1A1 Eukaryotic translation elongation factor 1 alpha 1 VA1-10418 NM_001615 Human ACTG2 actin, gamma 2, smooth muscle, enteric VA1-10437 NM_000680-N Human ADRA1A-Negative adrenergic, alpha-1A-, receptor VA1-10438 NM_001265 Human CDX2 caudal type homeobox 2 VA1-10441 NM_004348 Human RUNX2 Runt-related transcription factor 2 VA1-10449 NM_001844 Human COL2A1 Collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital) VA1-10450 NM_001135 Human AGC1 aggrecan1 VA1-10451 NM_000346 Human SOX9 SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal) VA1-10452 NM_006534 Human NCOA3 Nuclear receptor coactivator 3 VA1-10453 NM_017602 Human OTUD5 OTU domain containing 5 VA1-10456 NM_012242 Human DKK1 Dickkopf homolog 1 (Xenopus laevis) VA1-10478 NM_001200 Human BMP2 Bone morphogenetic protein 2 VA1-10479 NM_000594 Human TNF tumor necrosis factor (TNF superfamily, member 2) VA1-10481 QuantiGene FlowRNA Probe Sets Currently Available Accession No. Species Symbol Gene Name Catalog No. NM_001462 Human FPR2 formyl peptide receptor 2 VA1-10483 NM_002576 Human PAK1 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) VA1-10484 NM_003450 Human ZNF174 zinc finger protein 174 VA1-10511 NM_006460 Human HEXIM1 hexamethylene
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  • Supporting Online Material

    Supporting Online Material

    1 2 3 4 5 6 7 Supplementary Information for 8 9 Fractalkine-induced microglial vasoregulation occurs within the retina and is altered early in diabetic 10 retinopathy 11 12 *Samuel A. Mills, *Andrew I. Jobling, *Michael A. Dixon, Bang V. Bui, Kirstan A. Vessey, Joanna A. Phipps, 13 Ursula Greferath, Gene Venables, Vickie H.Y. Wong, Connie H.Y. Wong, Zheng He, Flora Hui, James C. 14 Young, Josh Tonc, Elena Ivanova, Botir T. Sagdullaev, Erica L. Fletcher 15 * Joint first authors 16 17 Corresponding author: 18 Prof. Erica L. Fletcher. Department of Anatomy & Neuroscience. The University of Melbourne, Grattan St, 19 Parkville 3010, Victoria, Australia. 20 Email: [email protected] ; Tel: +61-3-8344-3218; Fax: +61-3-9347-5219 21 22 This PDF file includes: 23 24 Supplementary text 25 Figures S1 to S10 26 Tables S1 to S7 27 Legends for Movies S1 to S2 28 SI References 29 30 Other supplementary materials for this manuscript include the following: 31 32 Movies S1 to S2 33 34 35 36 1 1 Supplementary Information Text 2 Materials and Methods 3 Microglial process movement on retinal vessels 4 Dark agouti rats were anaesthetized, injected intraperitoneally with rhodamine B (Sigma-Aldrich) to label blood 5 vessels and retinal explants established as described in the main text. Retinal microglia were labelled with Iba-1 6 and imaging performed on an inverted confocal microscope (Leica SP5). Baseline images were taken for 10 7 minutes, followed by the addition of PBS (10 minutes) and then either fractalkine or fractalkine + candesartan 8 (10 minutes) using concentrations outlined in the main text.
  • Supplementary Table 3 Complete List of RNA-Sequencing Analysis of Gene Expression Changed by ≥ Tenfold Between Xenograft and Cells Cultured in 10%O2

    Supplementary Table 3 Complete List of RNA-Sequencing Analysis of Gene Expression Changed by ≥ Tenfold Between Xenograft and Cells Cultured in 10%O2

    Supplementary Table 3 Complete list of RNA-Sequencing analysis of gene expression changed by ≥ tenfold between xenograft and cells cultured in 10%O2 Expr Log2 Ratio Symbol Entrez Gene Name (culture/xenograft) -7.182 PGM5 phosphoglucomutase 5 -6.883 GPBAR1 G protein-coupled bile acid receptor 1 -6.683 CPVL carboxypeptidase, vitellogenic like -6.398 MTMR9LP myotubularin related protein 9-like, pseudogene -6.131 SCN7A sodium voltage-gated channel alpha subunit 7 -6.115 POPDC2 popeye domain containing 2 -6.014 LGI1 leucine rich glioma inactivated 1 -5.86 SCN1A sodium voltage-gated channel alpha subunit 1 -5.713 C6 complement C6 -5.365 ANGPTL1 angiopoietin like 1 -5.327 TNN tenascin N -5.228 DHRS2 dehydrogenase/reductase 2 leucine rich repeat and fibronectin type III domain -5.115 LRFN2 containing 2 -5.076 FOXO6 forkhead box O6 -5.035 ETNPPL ethanolamine-phosphate phospho-lyase -4.993 MYO15A myosin XVA -4.972 IGF1 insulin like growth factor 1 -4.956 DLG2 discs large MAGUK scaffold protein 2 -4.86 SCML4 sex comb on midleg like 4 (Drosophila) Src homology 2 domain containing transforming -4.816 SHD protein D -4.764 PLP1 proteolipid protein 1 -4.764 TSPAN32 tetraspanin 32 -4.713 N4BP3 NEDD4 binding protein 3 -4.705 MYOC myocilin -4.646 CLEC3B C-type lectin domain family 3 member B -4.646 C7 complement C7 -4.62 TGM2 transglutaminase 2 -4.562 COL9A1 collagen type IX alpha 1 chain -4.55 SOSTDC1 sclerostin domain containing 1 -4.55 OGN osteoglycin -4.505 DAPL1 death associated protein like 1 -4.491 C10orf105 chromosome 10 open reading frame 105 -4.491