Nails and Hair
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Development and Maintenance of Epidermal Stem Cells in Skin Adnexa
International Journal of Molecular Sciences Review Development and Maintenance of Epidermal Stem Cells in Skin Adnexa Jaroslav Mokry * and Rishikaysh Pisal Medical Faculty, Charles University, 500 03 Hradec Kralove, Czech Republic; [email protected] * Correspondence: [email protected] Received: 30 October 2020; Accepted: 18 December 2020; Published: 20 December 2020 Abstract: The skin surface is modified by numerous appendages. These structures arise from epithelial stem cells (SCs) through the induction of epidermal placodes as a result of local signalling interplay with mesenchymal cells based on the Wnt–(Dkk4)–Eda–Shh cascade. Slight modifications of the cascade, with the participation of antagonistic signalling, decide whether multipotent epidermal SCs develop in interfollicular epidermis, scales, hair/feather follicles, nails or skin glands. This review describes the roles of epidermal SCs in the development of skin adnexa and interfollicular epidermis, as well as their maintenance. Each skin structure arises from distinct pools of epidermal SCs that are harboured in specific but different niches that control SC behaviour. Such relationships explain differences in marker and gene expression patterns between particular SC subsets. The activity of well-compartmentalized epidermal SCs is orchestrated with that of other skin cells not only along the hair cycle but also in the course of skin regeneration following injury. This review highlights several membrane markers, cytoplasmic proteins and transcription factors associated with epidermal SCs. Keywords: stem cell; epidermal placode; skin adnexa; signalling; hair pigmentation; markers; keratins 1. Epidermal Stem Cells as Units of Development 1.1. Development of the Epidermis and Placode Formation The embryonic skin at very early stages of development is covered by a surface ectoderm that is a precursor to the epidermis and its multiple derivatives. -
Abnormal Hair Follicle Development and Altered Cell Fate of Follicular
ERRATUM Development 137, 1775 (2010) doi:10.1242/dev.053116 Abnormal hair follicle development and altered cell fate of follicular keratinocytes in transgenic mice expressing Np63 Rose-Anne Romano, Kirsten Smalley, Song Liu and Satrajit Sinha There was a technical problem with the ePress version of Development 137, 1431-1439 published on 24 March 2010. A number of Greek symbols were missing from the text and figure headings in the Results section. A replacement ePress article was published on 31 March 2010. The online issue and print copy are correct. We apologise to authors and readers for this error. DEVELOPMENT AND STEM CELLS RESEARCH ARTICLE 1431 Development 137, 1431-1439 (2010) doi:10.1242/dev.045427 © 2010. Published by The Company of Biologists Ltd Abnormal hair follicle development and altered cell fate of follicular keratinocytes in transgenic mice expressing DNp63a Rose-Anne Romano1, Kirsten Smalley1, Song Liu2,3 and Satrajit Sinha1,* SUMMARY The transcription factor p63 plays an essential role in epidermal morphogenesis. Animals lacking p63 fail to form many ectodermal organs, including the skin and hair follicles. Although the indispensable role of p63 in stratified epithelial skin development is well established, relatively little is known about this transcriptional regulator in directing hair follicle morphogenesis. Here, using specific antibodies, we have established the expression pattern of DNp63 in hair follicle development and cycling. DNp63 is expressed in the developing hair placode, whereas in mature hair its expression is restricted to the outer root sheath (ORS), matrix cells and to the stem cells of the hair follicle bulge. To investigate the role of DNp63 in hair follicle morphogenesis and cycling, we have utilized a Tet-inducible mouse model system with targeted expression of this isoform to the ORS of the hair follicle. -
Anatomy and Physiology of Hair
Chapter 2 Provisional chapter Anatomy and Physiology of Hair Anatomy and Physiology of Hair Bilgen Erdoğan ğ AdditionalBilgen Erdo informationan is available at the end of the chapter Additional information is available at the end of the chapter http://dx.doi.org/10.5772/67269 Abstract Hair is one of the characteristic features of mammals and has various functions such as protection against external factors; producing sebum, apocrine sweat and pheromones; impact on social and sexual interactions; thermoregulation and being a resource for stem cells. Hair is a derivative of the epidermis and consists of two distinct parts: the follicle and the hair shaft. The follicle is the essential unit for the generation of hair. The hair shaft consists of a cortex and cuticle cells, and a medulla for some types of hairs. Hair follicle has a continuous growth and rest sequence named hair cycle. The duration of growth and rest cycles is coordinated by many endocrine, vascular and neural stimuli and depends not only on localization of the hair but also on various factors, like age and nutritional habits. Distinctive anatomy and physiology of hair follicle are presented in this chapter. Extensive knowledge on anatomical and physiological aspects of hair can contribute to understand and heal different hair disorders. Keywords: hair, follicle, anatomy, physiology, shaft 1. Introduction The hair follicle is one of the characteristic features of mammals serves as a unique miniorgan (Figure 1). In humans, hair has various functions such as protection against external factors, sebum, apocrine sweat and pheromones production and thermoregulation. The hair also plays important roles for the individual’s social and sexual interaction [1, 2]. -
Hair Histology As a Tool for Forensic Identification of Some Domestic Animal Species
EXCLI Journal 2018;17:663-670 – ISSN 1611-2156 Received: June 28, 2018, accepted: July 02, 2018, published: July 06, 2018 Original article: HAIR HISTOLOGY AS A TOOL FOR FORENSIC IDENTIFICATION OF SOME DOMESTIC ANIMAL SPECIES Yasser A. Ahmed1, Safwat Ali2, Ahmed Ghallab3* 1 Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt 2 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Minia University, Minia, Egypt 3 Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt * Corresponding author: E-mail: [email protected] http://dx.doi.org/10.17179/excli2018-1478 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). ABSTRACT Animal hair examination at a criminal scene may provide valuable information in forensic investigations. How- ever, local reference databases for animal hair identification are rare. In the present study, we provide differential histological analysis of hair of some domestic animals in Upper Egypt. For this purpose, guard hair of large rumi- nants (buffalo, camel and cow), small ruminants (sheep and goat), equine (horse and donkey) and canine (dog and cat) were collected and comparative analysis was performed by light microscopy. Based on the hair cuticle scale pattern, type and diameter of the medulla, and the pigmentation, characteristic differential features of each animal species were identified. The cuticle scale pattern was imbricate in all tested animals except in donkey, in which coronal scales were identified. The cuticle scale margin type, shape and the distance in between were characteristic for each animal species. -
Nail Anatomy and Physiology for the Clinician 1
Nail Anatomy and Physiology for the Clinician 1 The nails have several important uses, which are as they are produced and remain stored during easily appreciable when the nails are absent or growth. they lose their function. The most evident use of It is therefore important to know how the fi ngernails is to be an ornament of the hand, but healthy nail appears and how it is formed, in we must not underestimate other important func- order to detect signs of pathology and understand tions, such as the protective value of the nail plate their pathogenesis. against trauma to the underlying distal phalanx, its counterpressure effect to the pulp important for walking and for tactile sensation, the scratch- 1.1 Nail Anatomy ing function, and the importance of fi ngernails and Physiology for manipulation of small objects. The nails can also provide information about What we call “nail” is the nail plate, the fi nal part the person’s work, habits, and health status, as of the activity of 4 epithelia that proliferate and several well-known nail features are a clue to sys- differentiate in a specifi c manner, in order to form temic diseases. Abnormal nails due to biting or and protect a healthy nail plate [1 ]. The “nail onychotillomania give clues to the person’s emo- unit” (Fig. 1.1 ) is composed by: tional/psychiatric status. Nail samples are uti- • Nail matrix: responsible for nail plate production lized for forensic and toxicology analysis, as • Nail folds: responsible for protection of the several substances are deposited in the nail plate nail matrix Proximal nail fold Nail plate Fig. -
Nestin Expression in Hair Follicle Sheath Progenitor Cells
Nestin expression in hair follicle sheath progenitor cells Lingna Li*, John Mignone†, Meng Yang*, Maja Matic‡, Sheldon Penman§, Grigori Enikolopov†, and Robert M. Hoffman*¶ *AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111; †Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724; §Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4307; and ‡Stony Brook University, Stony Brook, NY 11794 Contributed by Sheldon Penman, June 25, 2003 The intermediate filament protein, nestin, marks progenitor expression of the neural stem cell protein nestin in hair follicle cells of the CNS. Such CNS stem cells are selectively labeled by stem cells suggests a possible relation. placing GFP under the control of the nestin regulatory se- quences. During early anagen or growth phase of the hair Materials and Methods follicle, nestin-expressing cells, marked by GFP fluorescence in Nestin-GFP Transgenic Mice. Nestin is an intermediate filament nestin-GFP transgenic mice, appear in the permanent upper hair (IF) gene that is a marker for CNS progenitor cells and follicle immediately below the sebaceous glands in the follicle neuroepithelial stem cells (5). Enhanced GFP (EGFP) trans- bulge. This is where stem cells for the hair follicle outer-root genic mice carrying EGFP under the control of the nestin sheath are thought to be located. The relatively small, oval- second-intron enhancer are used for studying and visualizing shaped, nestin-expressing cells in the bulge area surround the the self-renewal and multipotency of CNS stem cells (5–7). hair shaft and are interconnected by short dendrites. The precise Here we report that hair follicle stem cells strongly express locations of the nestin-expressing cells in the hair follicle vary nestin as evidenced by nestin-regulated EGFP expression. -
Smooth Muscle A-Actin Is a Marker for Hair Follicle Dermis in Vivoand in Vitro
Smooth muscle a-actin is a marker for hair follicle dermis in vivo and in vitro COLIN A. B. JAHODA1*, AMANDA J. REYNOLDS2•*, CHRISTINE CHAPONNIER2, JAMES C. FORESTER3 and GIULIO GABBIANI* 1Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, Scotland ^Department of Pathology, University of Geneva, 1211 Geneva 4, Switzerland ^Department of Surgery, Ninewells Hospital and Medical School, Dundee, Scotland * Present address: Department of Biological Sciences, University of Durham, Durham DH1 3LE, England Summary We have examined the expression of smooth muscle cells contained significant quantities of the a-actin a-actin in hair follicles in situ, and in hair follicle isoform. dermal cells in culture by means of immunohisto- The rapid switching on of smooth muscle a-actin chemistry. Smooth muscle a-actin was present in the expression by dermal papilla cells in early culture, dermal sheath component of rat vibrissa, rat pelage contrasts with the behaviour of smooth muscle cells and human follicles. Dermal papilla cells within all in vitro, and has implications for control of ex- types of follicles did not express the antigen. How- pression of the antigen in normal adult systems. The ever, in culture a large percentage of both hair very high percentage of positively marked cultured dermal papilla and dermal sheath cells were stained papilla and sheath cells also provides a novel marker by this antibody. The same cells were negative when of cells from follicle dermis, and reinforces the idea tested with an antibody to desmin. Overall, explant- that they represent a specialized cell population, derived skin fibroblasts had relatively low numbers contributing to the heterogeneity of fibroblast cell of positively marked cells, but those from skin types in the skin dermis, and possibly acting as a regions of high hair-follicle density displayed more source of myofibroblasts during wound healing. -
Identification of Hair Shaft Progenitors That Create a Niche for Hair Pigmentation
Downloaded from genesdev.cshlp.org on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press Identification of hair shaft progenitors that create a niche for hair pigmentation Chung-Ping Liao,1 Reid C. Booker,1 Sean J. Morrison,2,3,4,5,6 and Lu Q. Le1,4,5 1Department of Dermatology, 2Department of Pediatrics, 3Children’s Research Institute, 4Simmons Comprehensive Cancer Center, 5Hamon Center for Regenerative Science and Medicine, 6Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA Hair differentiates from follicle stem cells through progenitor cells in the matrix. In contrast to stem cells in the bulge, the identities of the progenitors and the mechanisms by which they regulate hair shaft components are poorly understood. Hair is also pigmented by melanocytes in the follicle. However, the niche that regulates follicular melanocytes is not well characterized. Here, we report the identification of hair shaft progenitors in the matrix that are differentiated from follicular epithelial cells expressing transcription factor KROX20. Depletion of Krox20 lin- eage cells results in arrest of hair growth, confirming the critical role of KROX20+ cells as antecedents of structural cells found in hair. Expression of stem cell factor (SCF) by these cells is necessary for the maintenance of differen- tiated melanocytes and for hair pigmentation. Our findings reveal the identities of hair matrix progenitors that regulate hair growth and pigmentation, partly by creating an SCF-dependent niche for follicular melanocytes. [Keywords: stem cell factor (SCF); hair pigmentation; hair shaft progenitor cell; hair follicle stem cell; hair matrix; KROX20] Supplemental material is available for this article. -
Neural Control of Facial Sweat Gland Secretion in Horses
Iowa State University Capstones, Theses and Creative Components Dissertations Fall 2019 Neural control of facial sweat gland secretion in horses Lu Liu Follow this and additional works at: https://lib.dr.iastate.edu/creativecomponents Part of the Comparative and Evolutionary Physiology Commons, and the Systems and Integrative Physiology Commons Recommended Citation Liu, Lu, "Neural control of facial sweat gland secretion in horses" (2019). Creative Components. 455. https://lib.dr.iastate.edu/creativecomponents/455 This Creative Component is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Creative Components by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Neural control of facial sweat gland secretion in horses Lu Liu 2019.11.26 BMS 599 Creative Component Biomedical Sciences Department Iowa State University Abstract: For sweat glands, it has been shown that sympathetic neurons express a cholinergic/noradrenergic co-phenotype in their innervation in the trunk of mice.(Schütz et al. 2008). It is unknown if facial sweat glands are innervated the same way. Gustatory sweating is an abnormal sweating condition. People can sweat profusely when eating or drinking, or even thinking about eating or drinking. We hypothesize that the facial sweat glands are controlled differently and can be related to parasympathetic neurons from cranial nerves. Some samples from human donors have been tested, but few sweat glands can be targeted since the donors were older people. Thus, it was decided to use horse tissue for this project to obtain greater numbers of sweat glands on the face. -
Revisiting Hair Follicle Embryology, Anatomy and the Follicular Cycle
etology sm & o C T r f i o c h l o a l n o r Silva et al., J Cosmo Trichol 2019, 5:1 g u y o J Journal of Cosmetology & Trichology DOI: 10.4172/2471-9323.1000141 ISSN: 2471-9323 Review Article Open Access Revisiting Hair Follicle Embryology, Anatomy and the Follicular Cycle Laura Maria Andrade Silva1*, Ricardo Hsieh2, Silvia Vanessa Lourenço3, Bruno de Oliveira Rocha1, Ricardo Romiti4, Neusa Yuriko Sakai Valente4,5, Alessandra Anzai4 and Juliana Dumet Fernandes1 1Department of Dermatology, Magalhães Neto Outpatient Clinic, Professor Edgar Santos School Hospital Complex, The Federal University of Bahia (C-HUPES/UFBa), Salvador/BA-Brazil 2Institute of Tropical Medicine of São Paulo, The University of São Paulo (IMT/USP), São Paulo/SP-Brazil 3Department of Stomatology, School of Dentistry, The University of São Paulo (FO/USP), São Paulo/SP-Brazil 4Department of Dermatology, University of São Paulo Medical School, The University of São Paulo (FMUSP), São Paulo/SP-Brazil 5Department of Dermatology, Hospital of the Public Server of the State of São Paulo (IAMSPE), São Paulo/SP-Brazil *Corresponding author: Laura Maria Andrade Silva, Department of Dermatology, Magalhães Neto Outpatient Clinic, Professor Edgar Santos School Hospital Complex, The Federal University of Bahia (C-HUPES/UFBa) Salvador/BA-Brazil, Tel: +55(71)3283-8380; +55(71)99981-7759; E-mail: [email protected] Received date: January 17, 2019; Accepted date: March 07, 2019; Published date: March 12, 2019 Copyright: © 2019 Silva LMA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. -
Hair Follicle Dermal Papilla Cells at a Glance
Cell Science at a Glance 1179 Hair follicle dermal interactions during development and in embryonic epidermis, which is detectable at postnatal life (Blanpain and Fuchs, 2009; embryonic day 14.5 (E14.5) of mouse papilla cells at a glance Müller-Röver et al., 2001; Schmidt-Ullrich and development. Soon after, a local condensation Paus, 2005; Watt and Jensen, 2009). One (dermal condensate) of fibroblasts forms Ryan R. Driskell1, Carlos Clavel2, population of mesenchymal cells in the skin, beneath the placode. Reciprocal signalling Michael Rendl2,* and Fiona M. known as dermal papilla (DP) cells, is the focus between the condensate and the placode leads Watt1,3,* of intense interest because the DP not only to proliferation of the overlying epithelium and 1Laboratory for Epidermal Stem Cell Biology, regulates hair follicle development and growth, downward extension of the new follicle into Wellcome Trust Centre for Stem Cell Research, but is also thought to be a reservoir of multi- the dermis (Millar, 2002; Schneider et al., University of Cambridge, Cambridge CB2 1QR, UK potent stem cells. In this article and the 2009; Ohyama et al., 2010; Yang and 2Black Family Stem Cell Institute and Department of Developmental and Regenerative Biology, Mount accompanying poster we review the origins of Cotsarelis, 2010). After the initial downward Sinai School of Medicine, New York, NY 10029, USA the DP during skin development, and discuss DP growth, the epithelial cells envelope the dermal 3CRUK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK heterogeneity and the changes in the DP that condensate, thereby forming the mature DP. -
The Integumentary System
CHAPTER 5: THE INTEGUMENTARY SYSTEM Copyright © 2010 Pearson Education, Inc. OVERALL SKIN STRUCTURE 3 LAYERS Copyright © 2010 Pearson Education, Inc. Figure 5.1 Skin structure. Hair shaft Dermal papillae Epidermis Subpapillary vascular plexus Papillary layer Pore Appendages of skin Dermis Reticular • Eccrine sweat layer gland • Arrector pili muscle Hypodermis • Sebaceous (oil) gland (superficial fascia) • Hair follicle Nervous structures • Hair root • Sensory nerve fiber Cutaneous vascular • Pacinian corpuscle plexus • Hair follicle receptor Adipose tissue (root hair plexus) Copyright © 2010 Pearson Education, Inc. EPIDERMIS 4 (or 5) LAYERS Copyright © 2010 Pearson Education, Inc. Figure 5.2 The main structural features of the skin epidermis. Keratinocytes Stratum corneum Stratum granulosum Epidermal Stratum spinosum dendritic cell Tactile (Merkel) Stratum basale Dermis cell Sensory nerve ending (a) Dermis Desmosomes Melanocyte (b) Melanin granule Copyright © 2010 Pearson Education, Inc. DERMIS 2 LAYERS Copyright © 2010 Pearson Education, Inc. Figure 5.3 The two regions of the dermis. Dermis (b) Papillary layer of dermis, SEM (22,700x) (a) Light micrograph of thick skin identifying the extent of the dermis, (50x) (c) Reticular layer of dermis, SEM (38,500x) Copyright © 2010 Pearson Education, Inc. Figure 5.3a The two regions of the dermis. Dermis (a) Light micrograph of thick skin identifying the extent of the dermis, (50x) Copyright © 2010 Pearson Education, Inc. Q1: The type of gland which secretes its products onto a surface is an _______ gland. 1) Endocrine 2) Exocrine 3) Merocrine 4) Holocrine Copyright © 2010 Pearson Education, Inc. Q2: The embryonic tissue which gives rise to muscle and most connective tissue is… 1) Ectoderm 2) Endoderm 3) Mesoderm Copyright © 2010 Pearson Education, Inc.