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Mycosphere New species and new records of cercosporoid hyphomycetes from Cuba and Venezuela (Part 3) Braun U1* and Urtiaga R2 1Martin-Luther-Universität, Institut für Biologie, Bereich Geobotanik und Botanischer Garten, Herbarium, Neuwerk 21, 06099 Halle (Saale), Germany 2Apartado 546, Barquisimeto, Lara, Venezuela. Braun U, Urtiaga R 2013 – New species and new records of cercosporoid hyphomycetes from Cuba and Venezuela (Part 3). Mycosphere x(x), xx–xx. Doi xxx/mycosphere/x/x/x Examinations of specimens of cercosporoid leaf-spotting hyphomycetes made between 1966 and 1997 in Cuba and Venezuela, now housed at K (previously deposited at IMI as “Cercospora sp.”), have been continued, supplemented by several samples collected in Venezuela between 2006 and 2012, which are now deposited at HAL. Some species are new to Cuba and Venezuela, some new host plants are included, and the following new species are introduced: Cercospora syngoniicola, Pseudocercospora apeibae, P. clematidis-haenkeanae, P. erythrinicola, P. erythroxylicola, P. guanarensis, P. helicteris, P. perseae-americanae, P. simirae, and Zasmidium cassiae-grandis. The new combination Pseudocercospora angraeci and the new name P. ranjita var. amphigena are proposed. Key words – Ascomycota – Mycosphaerellaceae – Cercospora – Cercosporella – Passalora – Pseudocercospora – Zasmidium – South America – West Indies Article Information Received *** Accepted *** Published online **** *Corresponding author: U. Braun – e-mail – [email protected] Introduction (Arnold 1986, Castañeda & Braun 1989, Braun Braun & Urtiaga (2008, 2012, 2013) and & Castañeda 1991, Vilaró Carmino et al. 2006) Braun et al. (2010) published results of exami- and Venezuela (Chupp 1934, Dennis 1976, nations of collections of cercosporoid, mostly Pons 1984, 1988, 1993, 2004, 2007, Urtiaga leaf-spotting hyphomycetes from Cuba and 1986, García et al. 1996, Itturiaga & Minter Venezuela, which are continued in the present 2006). Older data are also summarized in paper. The material concered was collected by Crous & Braun (2003). R. Urtiaga between 1966 and 1996 and later deposited at IMI as Cercospora sp. (recently Methods completely transferred to K). These specimens Sporulating structures were mounted in have recently been sent on loan to the first distilled water without any staining, and author to be determined and for further examined using oil immersion (bright field and treatment. Additional Venezuelan collections phase contrast), with standard light microscopy made between 2006 and 2012 have been (Olympus BX 50, Hamburg, Germany). Thirty directly sent to the first author and are now measurements ( 1000 magnification) of coni- deposited at HAL. dia and other structures were made, with the Results of these examinations supple- extremes given in parentheses. All drawings ment hitherto existing contributions to the have been prepared by the first author. knowledge of cercosporoid fungi of Cuba 1 Results and discussion 1967, R. Urtiaga 962 (IMI 129576 = K(M) New records of cercosporoid hyphomy- 173073); Bayamo, on living leaves of Dianthus cetes from Cuba and Venezuela and descrip- caryophyllus L. (Caryophyllaceae), 28 Sep. tions of new species are listed in alphabetical 1967, R. Urtiaga 904 (IMI 129454 = K(M) order by genus and species. Discussions and 173053). VENEZUELA, Lara, Duaca zone, on comments are added to each taxon. living leaves of Milleria quinqueflora L. (Asteraceae), Nov. 1993, R. Urtiaga (IMI Cercospora ageraticola Goh & W.H. Hsieh (= 361861 = K(M) 180152); on living leaves of C. apii complex [s. lat.]) Philodendron sp. (Araceae), Guanare, Corozal, Material examined – VENEZUELA, June 1990, R. Urtiaga (IMI 344203 = K(M) Lara, Villanueva, on leaves of Chromolaena 180154). laevigata (Lam.) R.M. King & A. Rob. [ Notes – Milleria quinqueflora and Eupatorium laevigatum Lam.] (Asteraceae), Philodendron sp. are new host species for C. Nov. 2008, R. Urtiaga (HAL 2350 F); apii s. lat. Cananga odorata and Dianthus Carabobo, Chirgua, Hacienda Mnte Sarco, on caryophyllus were recorded from Cuba as hosts Condylidium iresinoides (Kunth) R.M. King & of Cercospora sp. (Urtiaga 1886, Mercado Sierra et al. 1997, Vilaró Camino et al. 2006). H. Rob. [ Eupatorium iresinoides Kunth] Collections on Dianthus were described as (Asteraceae), May 2001, R. Urtiaga 357 (HAL Cercospora dianthi A.S. Mull. & Chupp. 2576 F). However, this name is invalid and was reduced Notes – The collection on Chromolaena to synonym with C. apii s. lat. by Crous & laevigata was originally identified as Calea sp. Braun (2003). and recorded by Braun et al. (2010) as Cercospora caleifolia Bat., J. Upadhyay & Cercospora brachiata Ellis & Everh. Netto. Morphological differences between the Material examined – CUBA, Bayamo, on collection from Venezuela and the type living leaves of Amaranthus crassipes Schltdl. collection from Brazil were discussed. The (Amaranthaceae), 12 Nov. 1966, R. Urtiaga identity of the host plant was checked by (IMI 123565 = K(M) 173069). botanists from the herbarium in Maracay and Notes – Amaranthus crassipes was corrected to Chromolaena laevigata. The recorded from Cuba as host of Cercospora sp. present collection belongs to the C. apii in Urtiaga (1986). complex (C. apii s. lat.) in the sense of Crous & Braun (2003). The name C. ageraticola is Cercospora echinochloae Davis available for collections on Eupatorium and Material examined – VENEZUELA, allied genera (see Guo et al. 2005). The without locality, on living leaves of collection on Condylidium iresinoides is Echinochloa colona (L.) Link (Poaceae), 14 characterized by having small, brown stromata, Mar. 1969, R. Urtiaga 201 (IMI 139295 = 10–40 µm diam., fasciculate conidiophores, K(M) 180150). 30–150 3–6 µm, up to 8 µm wide near the Notes – Known from Venezuela (Dennis base, pluriseptate, pale to medium brown, paler 1970, Crous & Braun 2003). towards the tip, conidogenous ells integrated, terminal and often also intercalary, Cercospora oxalidis A.S. Mull. & Chupp ex U. conidiogenous loci 2–3 µm diam., condia Braun & Crous aciculate, hyaline, up to 100 3–4 µm. C. Material examined – VENEZUELA, La- ageraticola is new to Venezuela, and the two ra, Sanare, Sabana Redonda Arriba, on living hosts are also new for this species. leaves of Oxalis corniculata L. [ Xanthoxalis corniculata (L.) Small] (Oxalidaceae), June Cercospora apii Fresen. s. lat. (C. apii 2009, R. Urtiaga 281 (HAL 2582 F). complex, sensu Crous & Braun 2003) Notes – New to Venezuela on a new host Material examined – CUBA, Bayamo, on species (hitherto only known from Brazil and living leaves of Cananga odorata (Lam.) USA on Oxalis sp., see Crous & Braun (2003). Hook. f. & Thomson (Annonaceae), 12 Oct. 2 Mycosphere Cercospora rottboeliigena Y.L. Guo & Y. Jiang conidiophores are much longer (up to 300 µm) Material examined – VENEZUELA, Ya- and the conidia are 50–250 µm long (Chupp racuy, on living leaves of Rottboelia exaltata L. 1954). C. alocasiae Goh & W.H. Hsieh, C. f. (Poaceae), 29 Mar. 1969, R. Urtiaga 272 aracearum Firdousi, A.N. Rai & K.M. Vyas, C. (IMI 139322 = K(M) 180151). caladii Cooke, C. colocasigena S. Narayan, Notes – New to Venezuela. Recorded in Kharwar, R.K. Singh & Bhartiya, and C. Urtiaga (1986) as Cercospora sp. monsterae S. Narayan, Kharwar & R.K. Singh are characterized by their consistently acicular, Cercospora syngoniicola U. Braun & Urtiaga, narrower conidia and belong to the C. apii sp. nov. Fig. 1 complex (Chupp 1954, Firdousi et al. 1991, MycoBank, MB xxxx. Hsieh & Goh 1990, Narayan et al. 1997, 2001), Etymology – derived from the host C. pistiae has very long conidiophores, up to genus, Syngonium. 250 µm (Nag Raj et al. 1971), C. chevalieri Cercosporae xanthosomatis similis sed Sacc. differs in having very long maculis distinctis, ad 18 mm diam., conidiis conidiophores, up to 250 µm, and broader brevioribus, ad 120 µm longis, hyalinis. conidia, 5–7 µm wide (Chupp 1954, Ellis Leaf spots amphigenous, subcircular to 1976), C. amorphophallicola U. Braun has angular-irregular, 2–18 mm diam., brownish, narrower conidia, 2–3.5 µm (Braun 2001a), olivaceous-brown, greyish to medium brown, and the conidia of C. arisematis F.L. Tai turn later with paler centre, greyish brown to pale olivaceous with age (Chupp 1954, Guo et greyish white, with a narrow darker border or al. 2005). diffuse brownish halo, occasionally somewhat zonate. Caespituli amphigenous, mostly epi- phyllous, finely punctiform, dark. Mycelium Fig. 1 – Cercospora syngoniicola. Based on internal. Stromata lacking or small immersed type material. a Conidiophore fascicles. b aggregations of swollen hyphal cells, 10–25 Solitary conidiophores arising from superficial mm diam., olivaceous-brown, cells 2.5–11 µm hyphae. c Conidiophores. d Conidia. – Bar = diam. Conidiophores solitary or in small 10 µm. fascicles, 2–15, arising from immersed hyphae or stromata, erumpent, loose to dense, erect, Cercosporella virgaureae (Thüm.) Allesch. straight, subcylindrical or attenuated towards Material examined – VENEZUELA, La- the tip, geniculate-sinuous, unbranched, 10–90 ra, Sanare, Sabana Redonda Arriba, on living × 3–9 µm, 0–5-septate, pale to medium leaves of Conyza bonariensis (L.) Cronquist olivaceous-brown or yellowish brown, thin- (Asteraceae), May 2009, R. Urtiaga 343 (HAL walled, smooth; conidiogenous cells integrated, 2575 F). terminal or conidiophores reduced to conidio- Notes – Braun & Urtiaga (2013) genous cells, 10–30 µm long, conidiogenous published a first record of this species from loci inconspicuous, occasionally

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