Anti-Eef1e1 / AIMP3 Antibody (ARG42124)

Product datasheet [email protected]

ARG42124 Package: 100 μl anti-eEF1E1 / AIMP3 antibody Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody recognizes eEF1E1 / AIMP3

Tested Reactivity Hu, Ms

Tested Application FACS, ICC/IF, WB

Host Mouse

Clonality Monoclonal

Clone 1494CT639.147.31

Isotype IgG2b, kappa

Target Name eEF1E1 / AIMP3

Antigen Species Human

Immunogen Recombinant protein of Human eEF1E1 / AIMP3.

Conjugation Un-conjugated

Alternate Names Eukaryotic translation elongation factor 1 epsilon-1; Elongation factor p18; AIMP3; P18; Multisynthase complex auxiliary component p18; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3

Application Instructions

Application table Application Dilution

FACS 1:25

ICC/IF 1:25

WB 1:2000 - 1:4000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control HepG2

Calculated Mw 20 kDa

Observed Size ~ 18 kDa

Properties

Form Liquid

Purification Purification with Protein G.

Buffer PBS and 0.09% (W/V) Sodium azide.

Preservative 0.09% (W/V) Sodium azide

www.arigobio.com 1/3 Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol EEF1E1

Gene Full Name eukaryotic translation elongation factor 1 epsilon 1

Background This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010]

Function Positive modulator of ATM response to DNA damage. [UniProt]

Cellular Localization Cytoplasm. Cytoplasm, cytosol. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. [UniProt]

Images

ARG42124 anti-eEF1E1 / AIMP3 antibody ICC/IF image

Immunofluorescence: 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG42124 anti-eEF1E1 / AIMP3 antibody (green) at 1:25 dilution. Dylight® 554 Phalloidin (red) for cytoplasmic actin staining. DAPI (blue) for nuclear staining.

ARG42124 anti-eEF1E1 / AIMP3 antibody WB image

Western blot: 20 µg of HepG2 whole cell lysate stained with ARG42124 anti-eEF1E1 / AIMP3 antibody at 1:2000 - 1:4000 dilution.

www.arigobio.com 2/3 ARG42124 anti-eEF1E1 / AIMP3 antibody FACS image

Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein- protein interactions followed by ARG42124 anti-eEF1E1 / AIMP3 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Mouse IgG2b (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.