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Insight Into the Resistome and Quorum Sensing System of a Divergent Acinetobacter Pittii Isolate from 1 an Untouched Site Of
bioRxiv preprint doi: https://doi.org/10.1101/745182; this version posted November 27, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Insight into the resistome and quorum sensing system of a divergent Acinetobacter pittii isolate from 2 an untouched site of the Lechuguilla Cave 3 4 Han Ming Gan1,2,3*, Peter Wengert4 , Hazel A. Barton5, André O. Hudson4 and Michael A. Savka4 5 1 Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong 6 3220 ,Victoria, Australia 7 2 Deakin Genomics Centre, Deakin University, Geelong 3220 ,Victoria, Australia 8 3 School of Science, Monash University Malaysia, Bandar Sunway, 47500 Petaling Jaya, Selangor, 9 Malaysia 10 4 Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, USA 11 5 Department of Biology, University of Akron, Akron, Ohio, USA 12 *Corresponding author 13 Name: Han Ming Gan 14 Email: [email protected] 15 Key words 16 Acinetobacter, quorum sensing, antibiotic resistance 17 18 Abstract 19 Acinetobacter are Gram-negative bacteria belonging to the sub-phyla Gammaproteobacteria, commonly 20 associated with soils, animal feeds and water. Some members of the Acinetobacter have been 21 implicated in hospital-acquired infections, with broad-spectrum antibiotic resistance. Here we report the 22 whole genome sequence of LC510, an Acinetobacter species isolated from deep within a pristine 23 location of the Lechuguilla Cave. -
Uncommon Pathogens Causing Hospital-Acquired Infections in Postoperative Cardiac Surgical Patients
Published online: 2020-03-06 THIEME Review Article 89 Uncommon Pathogens Causing Hospital-Acquired Infections in Postoperative Cardiac Surgical Patients Manoj Kumar Sahu1 Netto George2 Neha Rastogi2 Chalatti Bipin1 Sarvesh Pal Singh1 1Department of Cardiothoracic and Vascular Surgery, CN Centre, All Address for correspondence Manoj K Sahu, MD, DNB, Department India Institute of Medical Sciences, Ansari Nagar, New Delhi, India of Cardiothoracic and Vascular Surgery, CTVS office, 7th floor, CN 2Infectious Disease, Department of Medicine, All India Institute of Centre, All India Institute of Medical Sciences, New Delhi-110029, Medical Sciences, Ansari Nagar, New Delhi, India India (e-mail: [email protected]). J Card Crit Care 2020;3:89–96 Abstract Bacterial infections are common causes of sepsis in the intensive care units. However, usually a finite number of Gram-negative bacteria cause sepsis (mostly according to the hospital flora). Some organisms such as Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus are relatively common. Others such as Stenotrophomonas maltophilia, Chryseobacterium indologenes, Shewanella putrefaciens, Ralstonia pickettii, Providencia, Morganella species, Nocardia, Elizabethkingia, Proteus, and Burkholderia are rare but of immense importance to public health, in view of the high mortality rates these are associated with. Being aware of these organisms, as the cause of hospital-acquired infections, helps in the prevention, Keywords treatment, and control of sepsis in the high-risk cardiac surgical patients including in ► uncommon pathogens heart transplants. Therefore, a basic understanding of when to suspect these organ- ► hospital-acquired isms is important for clinical diagnosis and initiating therapeutic options. This review infection discusses some rarely appearing pathogens in our intensive care unit with respect to ► cardiac surgical the spectrum of infections, and various antibiotics that were effective in managing intensive care unit these bacteria. -
Sonication Contribution to Identifying Prosthetic Joint Infection With
Birlutiu et al. BMC Musculoskeletal Disorders (2017) 18:311 DOI 10.1186/s12891-017-1678-y CASE REPORT Open Access Sonication contribution to identifying prosthetic joint infection with Ralstonia pickettii: a case report and review of the literature Rares Mircea Birlutiu1*, Mihai Dan Roman2, Razvan Silviu Cismasiu3, Sorin Radu Fleaca2, Crina Maria Popa4, Manuela Mihalache5 and Victoria Birlutiu6 Abstract Background: In the context of an increase number of primary and revision total hip and total knee arthroplasty performed yearly, an increased risk of complication is expected. Prosthetic joint infection (PJI) remains the most common and feared arthroplasty complication. Ralstonia pickettii is a Gram-negative bacterium, that has also been identified in biofilms. It remains an extremely rare cause of PJI. There is no report of an identification of R. pickettii on an extracted spacer loaded with antibiotic. Case presentation: We present the case of an 83-years-old Caucasian male patient, that underwent a right cemented total hip replacement surgery. The patient is diagnosed with an early PJI with no isolated microorganism. A debridement and change of mobile parts is performed. At the beginning of 2016, the patient in readmitted into the Orthopedic Department for sever, right abdominal and groin pain and elevated serum erythrocyte sedimentation rate and C-reactive protein. A joint aspiration is performed with a negative microbiological examination. A two-stage exchange with long interval management is adopted, and a preformed spacer loaded with gentamicin was implanted. In July 2016, based on the proinflammatory markers evolution, a shift a three-stage exchange strategy is decided. In September 2016, a debridement, and changing of the preformed spacer loaded with gentamicin with another was carried out. -
Microbial Communities in Placentas from Term Normal Pregnancy Exhibit Spatially Variable Profiles Lindsay A
Washington University School of Medicine Digital Commons@Becker Open Access Publications 2017 Microbial communities in placentas from term normal pregnancy exhibit spatially variable profiles Lindsay A. Parnell Washington University School of Medicine in St. Louis Catherine M. Briggs Washington University School of Medicine in St. Louis Bin Cao Washington University School of Medicine in St. Louis Omar Delannoy-Bruno Washington University School of Medicine in St. Louis Andrew E. Schrieffer Washington University School of Medicine in St. Louis See next page for additional authors Follow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubs Recommended Citation Parnell, Lindsay A.; Briggs, Catherine M.; Cao, Bin; Delannoy-Bruno, Omar; Schrieffer, Andrew E.; and Mysorekar, Indira U., ,"Microbial communities in placentas from term normal pregnancy exhibit spatially variable profiles." Scientific Reports.7,. (2017). https://digitalcommons.wustl.edu/open_access_pubs/6175 This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact [email protected]. Authors Lindsay A. Parnell, Catherine M. Briggs, Bin Cao, Omar Delannoy-Bruno, Andrew E. Schrieffer, and Indira U. Mysorekar This open access publication is available at Digital Commons@Becker: https://digitalcommons.wustl.edu/open_access_pubs/6175 www.nature.com/scientificreports OPEN Microbial communities in placentas from term normal pregnancy exhibit spatially variable profles Received: 30 January 2017 Lindsay A. Parnell1, Catherine M. Briggs1, Bin Cao1, Omar Delannoy-Bruno1, Andrew E. Accepted: 24 August 2017 Schriefer2 & Indira U. Mysorekar 1,3 Published: xx xx xxxx The placenta is the principal organ nurturing the fetus during pregnancy and was traditionally considered to be sterile. -
Cultivation of Microorganisms from Basaltic Rock
Edwards, K.J., Bach, W., Klaus, A., and the Expedition 336 Scientists Proceedings of the Integrated Ocean Drilling Program, Volume 336 Data report: cultivation of microorganisms from basaltic rock and sediment cores from the North Pond on the western flank of the Mid-Atlantic Ridge, IODP Expedition 3361 Hisako Hirayama,2 Mariko Abe,2 Junichi Miyazaki,2 Sanae Sakai,2 Yuriko Nagano,3 and Ken Takai2 Chapter contents Abstract Abstract . 1 Cultivation experiments targeting chemolithoautotrophic micro- organisms were performed using subseafloor basaltic cores (the Introduction . 1 deepest sample is from 315 meters below seafloor [mbsf] and Materials and methods . 2 overlying sediment cores (the deepest sample is from 91.4 mbsf) Results . 4 from North Pond on the western flank of the Mid-Atlantic Ridge. Acknowledgments. 5 The cores were recovered by the R/V JOIDES Resolution during Inte- References . 5 grated Ocean Drilling Program Expedition 336. Different bacteria Figure. 8 were grown under different media and temperature conditions. In Tables. 9 the enrichment cultures of the basaltic cores under aerobic condi- tions, frequently detected bacteria at 8°C and 25°C were members of the genera Ralstonia (the class Betaproteobacteria) and Pseudo- monas (Gammaproteobacteria), whereas members of the genera Paenibacillus (Bacilli) and Acidovorax (Betaproteobacteria) were conspicuous at 37°C. Bacillus spp. (Bacilli) were outstanding at 37°C under anaerobic conditions. In the enriched cultures of the sediment cores, bacterial growth was observed at 15°C but not at 37°C, and the bacteria detected at 15°C mostly belonged to gam- maproteobacterial genera such as Pseudomonas, Halomonas, and Marinobacter. -
Food Microbiology
Food Microbiology Food Water Dairy Beverage Online Ordering Available Food, Water, Dairy, & Beverage Microbiology Table of Contents 1 Environmental Monitoring Contact Plates 3 Petri Plates 3 Culture Media for Air Sampling 4 Environmental Sampling Boot Swabs 6 Environmental Testing Swabs 8 Surface Sanitizers 8 Hand Sanitation 9 Sample Preparation - Dilution Vials 10 Compact Dry™ 12 HardyCHROM™ Chromogenic Culture Media 15 Prepared Media 24 Agar Plates for Membrane Filtration 26 CRITERION™ Dehydrated Culture Media 28 Pathogen Detection Environmental With Monitoring Contact Plates Baird Parker Agar Friction Lid For the selective isolation and enumeration of coagulase-positive staphylococci (Staphylococcus aureus) on environmental surfaces. HardyCHROM™ ECC 15x60mm contact plate, A chromogenic medium for the detection, 10/pk ................................................................................ 89407-364 differentiation, and enumeration of Escherichia coli and other coliforms from environmental surfaces (E. coli D/E Neutralizing Agar turns blue, coliforms turn red). For the enumeration of environmental organisms. 15x60mm plate contact plate, The media is able to neutralize most antiseptics 10/pk ................................................................................ 89407-354 and disinfectants that may inhibit the growth of environmental organisms. Malt Extract 15x60mm contact plate, Malt Extract is recommended for the cultivation and 10/pk ................................................................................89407-482 -
Prepared Culture Media
PREPARED CULTURE MEDIA 121517SS PREPARED CULTURE MEDIA Made in the USA AnaeroGRO™ DuoPak A 02 Bovine Blood Agar, 5%, with Esculin 13 AnaeroGRO™ DuoPak B 02 Bovine Blood Agar, 5%, with Esculin/ AnaeroGRO™ BBE Agar 03 MacConkey Biplate 13 AnaeroGRO™ BBE/PEA 03 Bovine Selective Strep Agar 13 AnaeroGRO™ Brucella Agar 03 Brucella Agar with 5% Sheep Blood, Hemin, AnaeroGRO™ Campylobacter and Vitamin K 13 Selective Agar 03 Brucella Broth with 15% Glycerol 13 AnaeroGRO™ CCFA 03 Brucella with H and K/LKV Biplate 14 AnaeroGRO™ Egg Yolk Agar, Modified 03 Buffered Peptone Water 14 AnaeroGRO™ LKV Agar 03 Buffered Peptone Water with 1% AnaeroGRO™ PEA 03 Tween® 20 14 AnaeroGRO™ MultiPak A 04 Buffered NaCl Peptone EP, USP 14 AnaeroGRO™ MultiPak B 04 Butterfield’s Phosphate Buffer 14 AnaeroGRO™ Chopped Meat Broth 05 Campy Cefex Agar, Modified 14 AnaeroGRO™ Chopped Meat Campy CVA Agar 14 Carbohydrate Broth 05 Campy FDA Agar 14 AnaeroGRO™ Chopped Meat Campy, Blood Free, Karmali Agar 14 Glucose Broth 05 Cetrimide Select Agar, USP 14 AnaeroGRO™ Thioglycollate with Hemin and CET/MAC/VJ Triplate 14 Vitamin K (H and K), without Indicator 05 CGB Agar for Cryptococcus 14 Anaerobic PEA 08 Chocolate Agar 15 Baird-Parker Agar 08 Chocolate/Martin Lewis with Barney Miller Medium 08 Lincomycin Biplate 15 BBE Agar 08 CompactDry™ SL 16 BBE Agar/PEA Agar 08 CompactDry™ LS 16 BBE/LKV Biplate 09 CompactDry™ TC 17 BCSA 09 CompactDry™ EC 17 BCYE Agar 09 CompactDry™ YMR 17 BCYE Selective Agar with CAV 09 CompactDry™ ETB 17 BCYE Selective Agar with CCVC 09 CompactDry™ YM 17 BCYE -
22092 Tryptic Soy Broth (TSB, (Tryptone Soya Broth, CASO Broth, Soybean Casein Digest Broth, Casein Soya Broth)
22092 Tryptic Soy Broth (TSB, (Tryptone Soya Broth, CASO Broth, Soybean Casein digest Broth, Casein Soya Broth) The medium will support a luxuriant growth of many fastidious organisms without the addition of serum. Used for confirmation of Campylobacter jejuni by means of the motility test. Composition: Ingredients Grams/Litre Casein peptone (pancreatic) 17.0 Soya peptone (papain digest.) 3.0 Sodium chloride 5.0 Dipotassium hydrogen phosphate 2.5 Glucose 2.5 Final pH 7.3 +/- 0.2 at 25°C Store prepared media below 8°C, protected from direct light. Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C. Directions : Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Sterilize at 121°C for 15 minutes. Cool to 45- 50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes. Principle and Interpretation: Casein peptone and Soya peptone provide nitrogen, vitamins and minerals. The natural sugars from Soya peptone and Glucose promote organism growth. Sodium chloride is for the osmotic balance, while Dipotassium hydrogen phosphate is a buffering agent. Tryptone Soya Broth is often for the tube dilution method of antibiotic susceptibility testing. The addition of a small amount of agar ( approx. 0.05-0.2% 05040, add before sterilisation) renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium species. The superior growth-promoting properties of Tryptic Soy Broth make it especially useful for the isolation of organisms from blood or other body fluids. Anticoagulants such as sodium polyanetholesulfonate (81305) or sodium citrate (71635) may be added to the broth prior to sterilisation. -
Antimicrobial Susceptibility Patterns of Unusual Nonfermentative Gram
Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 100(6): 571-577, October 2005 571 Antimicrobial susceptibility patterns of unusual nonfermentative gram-negative bacilli isolated from Latin America: report from the SENTRY Antimicrobial Surveillance Program (1997-2002) Ana C Gales/+, Ronald N Jones*, Soraya S Andrade, Helio S Sader* Disciplina de Doenças Infecciosas e Parasitárias, Departamento de Medicina, Universidade Federal de São Paulo, Rua Leandro Dupret 188, 04025-010 São Paulo, SP, Brasil *The Jones Group/JMI Laboratories, North Liberty, IA, US The antimicrobial susceptibility of 176 unusual non-fermentative gram-negative bacilli (NF-GNB) collected from Latin America region through the SENTRY Program between 1997 and 2002 was evaluated by broth microdilution according to the National Committee for Clinical Laboratory Standards (NCCLS) recommendations. Nearly 74% of the NF-BGN belonged to the following genera/species: Burkholderia spp. (83), Achromobacter spp. (25), Ralstonia pickettii (16), Alcaligenes spp. (12), and Cryseobacterium spp. (12). Generally, trimethoprim/sulfamethoxazole (MIC50, ≤ 0.5 µg/ml) was the most potent drug followed by levofloxacin (MIC50, 0.5 µg/ml), and gatifloxacin (MIC50, 1 µg/ml). The highest susceptibility rates were observed for levofloxacin (78.3%), gatifloxacin (75.6%), and meropenem (72.6%). Ceftazidime (MIC50, 4 µg/ml; 83.1% susceptible) was the most active β-lactam against B. cepacia. Against Achro- mobacter spp. isolates, meropenem (MIC50, 0.25 µg/ml; 88% susceptible) was more active than imipenem (MIC50, 2 µg/ml). Cefepime (MIC50, 2 µg/ml; 81.3% susceptible), and imipenem (MIC50, 2 µg/ml; 81.3% susceptible) were more active than ceftazidime (MIC50, >16 µg/ml; 18.8% susceptible) and meropenem (MIC50, 8 µg/ml; 50% susceptible) against Ralstonia pickettii. -
A Comparative Study of Culture Methods and Polymerase Chain Reaction for Salmonella Detection in Egg Content
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content M. A. Soria ,* M. C. Soria ,*† and D. J. Bueno *1 * Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Concepción del Uruguay, Casilla de Correo No. 6, 3260, Entre Ríos, Argentina; and † Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Downloaded from https://academic.oup.com/ps/article-abstract/91/10/2668/1561306 by guest on 06 December 2019 ABSTRACT The present work compared 2 culture tion rates. All selective plating media did not show any methods and a PCR assay applied with 2 enrichment statistical differences in the parameters of performance methods for the detection of motile and nonmotile Sal- studied. Kappa coefficients showed that there was an monella strains using artificially contaminated egg con- excellent agreement between the bacteriological meth- tent. The specificity (Sp) was 1 in all methods. The ods for all Salmonella strains. The agreement was very sensitivity (Se), accuracy (Ac), positive predictive value good and good between the PCR methods, for motile (PPV), and negative predictive value (NPV) were 1 in and nonmotile strains, respectively. However, there both culture methods for motile and nonmotile strains. was a poor agreement when the PCR and bacteriologi- In reference to the PCR methods, Se and PPV were cal methods were compared for motile and nonmotile between 0 and 1, whereas Ac and NPV were between Salmonella strains. The TT and MKTTn methods are 0.14 and 1. The detection level of motile and nonmo- similar in terms of Ac, Se, Sp, PPV, and NPV for dif- tile strains was 5 to 54 cfu per 25 mL for both culture ferent Salmonella strains in egg content. -
Characterization of Cucumber Fermentation Spoilage Bacteria by Enrichment Culture and 16S Rdna Cloning
Characterization of Cucumber Fermentation Spoilage Bacteria by Enrichment Culture and 16S rDNA Cloning Fred Breidt, Eduardo Medina, Doria Wafa, Ilenys P´erez-D´ıaz, Wendy Franco, Hsin-Yu Huang, Suzanne D. Johanningsmeier, and Jae Ho Kim Abstract: Commercial cucumber fermentations are typically carried out in 40000 L fermentation tanks. A secondary fermentation can occur after sugars are consumed that results in the formation of acetic, propionic, and butyric acids, concomitantly with the loss of lactic acid and an increase in pH. Spoilage fermentations can result in significant economic loss for industrial producers. The microbiota that result in spoilage remain incompletely defined. Previous studies have implicated yeasts, lactic acid bacteria, enterobacteriaceae, and Clostridia as having a role in spoilage fermentations. We report that Propionibacterium and Pectinatus isolates from cucumber fermentation spoilage converted lactic acid to propionic acid, increasing pH. The analysis of 16S rDNA cloning libraries confirmed and expanded the knowledge gained from previous studies using classical microbiological methods. Our data show that Gram-negative anaerobic bacteria supersede Gram-positive Fermincutes species after the pH rises from around 3.2 to pH 5, and propionic and butyric acids are produced. Characterization of the spoilage microbiota is an important first step in efforts to prevent cucumber fermentation spoilage. Keywords: pickled vegetables, Pectinatus, Propionibacteria, secondary cucumber fermentation, spoilage M: Food Microbiology Practical Application: An understanding of the microorganisms that cause commercial cucumber fermentation spoilage & Safety may aid in developing methods to prevent the spoilage from occurring. Introduction cucumbers fermented at 2.3% NaCl (Fleming and others 1989). Commercial cucumber fermentations are typically carried out In this fermentation tank, the initial lactic acid fermentation was in large 40000 L outdoor tanks (reviewed by Breidt and others completed within 2 wk, with 1.2% lactic acid formed (pH 3.6) 2007). -
TRYPTIC SOY BROTH W/ 15% GLYCEROL
TRYPTIC SOY BROTH w/ 15% GLYCEROL INTENDED USE Remel Tryptic Soy Broth w/ 15% Glycerol is a liquid medium recommended for use in long-term storage of bacteria. SUMMARY AND EXPLANATION This medium is recommended in Clinical Microbiology Procedures Handbook and by Clinical and Laboratory Standards Institute for the maintenance of stock cultures.1,2 When inoculated into Tryptic Soy Broth w/ 15% Glycerol and frozen at or below -40°C, suspensions of bacteria remain viable for several months. PRINCIPLE Casein and soy peptones provide amino acids and nitrogenous compounds for the growth of bacteria. Sodium chloride is a source of essential electrolytes and maintains osmotic equilibrium. Dipotassium phosphate is a pH buffer. Dextrose is a carbon energy source. Glycerol is a cryoprotective agent that provides intracellular and extracellular protection against freezing and promotes long-term preservation. REAGENTS (CLASSICAL FORMULA)* Casein Peptone........................................................... 17.0 g Dipotassium Phosphate ................................................2.5 g Sodium Chloride............................................................ 5.0 g Dextrose ........................................................................2.5 g Soy Peptone.................................................................. 3.0 g Glycerol .....................................................................150.0 ml Demineralized Water.................................................850.0 ml pH 7.3 ± 0.2 @ 25°C *Adjusted as required to meet performance standards. PROCEDURE 1. Inoculate the medium with a pure culture using a sterile inoculating loop or swab. 2. Place the tube with cap tightened at or below -40°C. 3. Organisms that have been inoculated and frozen in this medium should be thawed and checked for viability at appropriate intervals, following established laboratory guidelines. QUALITY CONTROL All lot numbers of Tryptic Soy Broth w/ 15% Glycerol have been tested using the following quality control organisms and have been found to be acceptable.