P30 Laboratory Cultivation of Ciona and Other Tunicates
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Natural Products Diversity of Marine Ascidians (Tunicates; Ascidiacea) and Successful Drugs in Clinical Development
Nat. Prod. Bioprospect. DOI 10.1007/s13659-016-0115-5 REVIEW Natural Products Diversity of Marine Ascidians (Tunicates; Ascidiacea) and Successful Drugs in Clinical Development Satheesh Kumar Palanisamy . N. M. Rajendran . Angela Marino Received: 19 November 2016 / Accepted: 14 December 2016 Ó The Author(s) 2017. This article is published with open access at Springerlink.com Abstract This present study reviewed the chemical diversity of marine ascidians and their pharmacological applications, challenges and recent developments in marine drug discovery reported during 1994–2014, highlighting the structural activity of compounds produced by these specimens. Till date only 5% of living ascidian species were studied from\3000 species, this study represented from family didemnidae (32%), polyclinidae (22%), styelidae and polycitoridae (11–12%) exhibiting the highest number of promising MNPs. Close to 580 compound structures are here discussed in terms of their occurrence, structural type and reported biological activity. Anti-cancer drugs are the main area of interest in the screening of MNPs from ascidians (64%), followed by anti-malarial (6%) and remaining others. FDA approved ascidian compounds mechanism of action along with other compounds status of clinical trials (phase 1 to phase 3) are discussed here in. This review highlights recent developments in the area of natural products chemistry and biotechnological approaches are emphasized. Keywords Cancer Á Cytotoxicity Á Diversity Á Metabolites Á Pharmacology 1 Introduction from marine invertebrates, especially sponges, ascidians, bryozoans and molluscs in which some of them are The study of marine natural products (MNPs) is becoming approved by FDA and currently utilized in clinical trials ever more sophisticated and an increasingly collaborative [1]. -
De Novo Draft Assembly of the Botrylloides Leachii Genome
bioRxiv preprint doi: https://doi.org/10.1101/152983; this version posted June 21, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 De novo draft assembly of the Botrylloides leachii genome 2 provides further insight into tunicate evolution. 3 4 Simon Blanchoud1#, Kim Rutherford2, Lisa Zondag1, Neil Gemmell2 and Megan J Wilson1* 5 6 1 Developmental Biology and Genomics Laboratory 7 2 8 Department of Anatomy, School of Biomedical Sciences, University of Otago, P.O. Box 56, 9 Dunedin 9054, New Zealand 10 # Current address: Department of Zoology, University of Fribourg, Switzerland 11 12 * Corresponding author: 13 Email: [email protected] 14 Ph. +64 3 4704695 15 Fax: +64 479 7254 16 17 Keywords: chordate, regeneration, Botrylloides leachii, ascidian, tunicate, genome, evolution 1 bioRxiv preprint doi: https://doi.org/10.1101/152983; this version posted June 21, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 18 Abstract (250 words) 19 Tunicates are marine invertebrates that compose the closest phylogenetic group to the 20 vertebrates. This chordate subphylum contains a particularly diverse range of reproductive 21 methods, regenerative abilities and life-history strategies. Consequently, tunicates provide an 22 extraordinary perspective into the emergence and diversity of chordate traits. Currently 23 published tunicate genomes include three Phlebobranchiae, one Thaliacean, one Larvacean 24 and one Stolidobranchian. To gain further insights into the evolution of the tunicate phylum, 25 we have sequenced the genome of the colonial Stolidobranchian Botrylloides leachii. -
Ascidiacea (Chordata: Tunicata) of Greece: an Updated Checklist
Biodiversity Data Journal 4: e9273 doi: 10.3897/BDJ.4.e9273 Taxonomic Paper Ascidiacea (Chordata: Tunicata) of Greece: an updated checklist Chryssanthi Antoniadou‡, Vasilis Gerovasileiou§§, Nicolas Bailly ‡ Department of Zoology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece § Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece Corresponding author: Chryssanthi Antoniadou ([email protected]) Academic editor: Christos Arvanitidis Received: 18 May 2016 | Accepted: 17 Jul 2016 | Published: 01 Nov 2016 Citation: Antoniadou C, Gerovasileiou V, Bailly N (2016) Ascidiacea (Chordata: Tunicata) of Greece: an updated checklist. Biodiversity Data Journal 4: e9273. https://doi.org/10.3897/BDJ.4.e9273 Abstract Background The checklist of the ascidian fauna (Tunicata: Ascidiacea) of Greece was compiled within the framework of the Greek Taxon Information System (GTIS), an application of the LifeWatchGreece Research Infrastructure (ESFRI) aiming to produce a complete checklist of species recorded from Greece. This checklist was constructed by updating an existing one with the inclusion of recently published records. All the reported species from Greek waters were taxonomically revised and cross-checked with the Ascidiacea World Database. New information The updated checklist of the class Ascidiacea of Greece comprises 75 species, classified in 33 genera, 12 families, and 3 orders. In total, 8 species have been added to the previous species list (4 Aplousobranchia, 2 Phlebobranchia, and 2 Stolidobranchia). Aplousobranchia was the most speciose order, followed by Stolidobranchia. Most species belonged to the families Didemnidae, Polyclinidae, Pyuridae, Ascidiidae, and Styelidae; these 4 families comprise 76% of the Greek ascidian species richness. The present effort revealed the limited taxonomic research effort devoted to the ascidian fauna of Greece, © Antoniadou C et al. -
Redalyc.Keys for the Identification of Families and Genera of Atlantic
Biota Neotropica ISSN: 1676-0611 [email protected] Instituto Virtual da Biodiversidade Brasil Moreira da Rocha, Rosana; Bastos Zanata, Thais; Moreno, Tatiane Regina Keys for the identification of families and genera of Atlantic shallow water ascidians Biota Neotropica, vol. 12, núm. 1, enero-marzo, 2012, pp. 1-35 Instituto Virtual da Biodiversidade Campinas, Brasil Available in: http://www.redalyc.org/articulo.oa?id=199123750022 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Keys for the identification of families and genera of Atlantic shallow water ascidians Rocha, R.M. et al. Biota Neotrop. 2012, 12(1): 000-000. On line version of this paper is available from: http://www.biotaneotropica.org.br/v12n1/en/abstract?identification-key+bn01712012012 A versão on-line completa deste artigo está disponível em: http://www.biotaneotropica.org.br/v12n1/pt/abstract?identification-key+bn01712012012 Received/ Recebido em 16/07/2011 - Revised/ Versão reformulada recebida em 13/03/2012 - Accepted/ Publicado em 14/03/2012 ISSN 1676-0603 (on-line) Biota Neotropica is an electronic, peer-reviewed journal edited by the Program BIOTA/FAPESP: The Virtual Institute of Biodiversity. This journal’s aim is to disseminate the results of original research work, associated or not to the program, concerned with characterization, conservation and sustainable use of biodiversity within the Neotropical region. Biota Neotropica é uma revista do Programa BIOTA/FAPESP - O Instituto Virtual da Biodiversidade, que publica resultados de pesquisa original, vinculada ou não ao programa, que abordem a temática caracterização, conservação e uso sustentável da biodiversidade na região Neotropical. -
Cellular and Molecular Mechanisms of Regeneration in Colonial and Solitary MARK Ascidians ⁎ Susannah H
Developmental Biology 448 (2019) 271–278 Contents lists available at ScienceDirect Developmental Biology journal homepage: www.elsevier.com/locate/developmentalbiology Review article Cellular and molecular mechanisms of regeneration in colonial and solitary MARK Ascidians ⁎ Susannah H. Kassmer , Shane Nourizadeh, Anthony W. De Tomaso Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA, USA ABSTRACT Regenerative ability is highly variable among the metazoans. While many invertebrate organisms are capable of complete regeneration of entire bodies and organs, whole-organ regeneration is limited to very few species in the vertebrate lineages. Tunicates, which are invertebrate chordates and the closest extant relatives of the vertebrates, show robust regenerative ability. Colonial ascidians of the family of the Styelidae, such as several species of Botrylloides, are able to regenerate entire new bodies from nothing but fragments of vasculature, and they are the only chordates that are capable of whole body regeneration. The cell types and signaling pathways involved in whole body regeneration are not well understood, but some evidence suggests that blood borne cells may play a role. Solitary ascidians such as Ciona can regenerate the oral siphon and their central nervous system, and stem cells located in the branchial sac are required for this regeneration. Here, we summarize the cellular and molecular mechanisms of tunicate regeneration that have been identified so far and discuss differences and similarities -
Ecological Aspects of the Ascidian Community Along the Israeli Coasts
Ecological aspects of the ascidian community along the Israeli coasts THESIS SUBMITTED FOR THE DEGREE “DOCTOR OF PHILOSOPHY” BY Noa Shenkar SUBMITTED TO THE SENATE OF TEL-AVIV UNIVERSITY February 2008 This work was carried out under the supervision of Prof. Yossi Loya This work is dedicated with enormous love to Dror & little Ido תודות Acknowledgments I would like to express my gratitude to many people who helped me during this research. לפרופ' יוסי לויה שזכיתי להיות תלמידתו ולימד אותי מלבד אקולוגיה וביולוגיה ימית גם דבר או שניים על איך להיות בן - אדם. לחברי הועדה המלווה: פרופ' הודי בניהו, פרופ' יאיר אחיטוב ופרופ' אלי גפן שתמכו וייעצו ודלתם תמיד היתה פתוחה בפני . לד"ר אסתי וינטר שלימדה אותי לראות את הטוב בכל דבר . לפרופ' לב פישלזון שהתמזל מזלי להיות שכנתו ולימד אותי מהי זואולוגיה. To my colleagues abroad: To Charlie & Gretchen Lambert for their enthusiasm and love to ascidians. To Patricia Mather (née Kott) for her advice and support. To Elsa Vàzquez Otero, Rosana Moreira da Rocha and Françoise Monniot for teaching me ascidian taxonomy with great love and care. To Xavier Turon for his constructive remarks and to Amy Driskell for helping me with the PCR game. לחברי מעבדתי שליוו אותי לאורך השנים ועזרו בכל עת, ובמיוחד לעומרי בורנשטיין, אלן דניאל, מיה ויזל, עידו מזרחי, רועי סגל, רן סולם ומיכה רוזנפלד. לחברי מעבדת בניהו, יעל זלדמן, מתי הלפרין, ענבל גינסבורג ועידו סלע שתמיד יצאו בשמחה למשימות דיגום איצטלנים מולחברי עבדתו של פרופ' מיכה אילן על החברה והעוגיות . לד"ר איציק בריקנר על החתכים ההיסטולוגים המופלאים, ורדה ווכסלר על הגרפיקה, נעמי פז על העריכה וההגהה, אלכס שלגמן על העזרה הלבבית עם האוספים, וענת גלזר מחברת החשמל. -
Development of a Marine Sensitivity Mapping Database and GIS Integration
The Marine Life Information Network® for Britain and Ireland (MarLIN) Development of a marine sensitivity mapping database and GIS integration. Stage 1. Review of current habitat and species information Contract no. FC 73-02-245 Report to Cyngor Cefn Gwlad Cymru / Countryside Council for Wales Harvey Tyler-Walters Olwen Ager Keith Hiscock December 2002 Reference: Tyler-Walters, H., Ager, O.E.D. & Hiscock, K., 2002. Development of a marine sensitivity mapping database and GIS integration. Stage 1. Review of current habitat and species information. Report to Cyngor Cefn Gwlad Cymru / Countryside Council for Wales from the Marine Life Information Network (MarLIN). Plymouth: Marine Biological Association of the UK. [Contract no. FC 73-02-245] Sensitivity mapping: review of current habitat and species information MarLIN 2 Sensitivity mapping: review of current habitat and species information MarLIN Development of a marine sensitivity mapping database and GIS integration. Stage 1. Review of current habitat and species information. Contents 1. AIMS AND BACKGROUND TO CONTRACT........................................................................................................9 2. TIMETABLE.....................................................................................................................................................9 3. METHODOLOGY..............................................................................................................................................9 4. RESULTS .......................................................................................................................................................10 -
Metabarcoding Analysis on European Coastal Samples Reveals New
www.nature.com/scientificreports OPEN Metabarcoding analysis on European coastal samples reveals new molecular metazoan diversity Received: 8 November 2017 David López-Escardó1, Jordi Paps2, Colomban de Vargas3,4, Ramon Massana5, Accepted: 5 June 2018 Iñaki Ruiz-Trillo 1,6,7 & Javier del Campo1,5 Published: xx xx xxxx Although animals are among the best studied organisms, we still lack a full description of their diversity, especially for microscopic taxa. This is partly due to the time-consuming and costly nature of surveying animal diversity through morphological and molecular studies of individual taxa. A powerful alternative is the use of high-throughput environmental sequencing, providing molecular data from all organisms sampled. We here address the unknown diversity of animal phyla in marine environments using an extensive dataset designed to assess eukaryotic ribosomal diversity among European coastal locations. A multi-phylum assessment of marine animal diversity that includes water column and sediments, oxic and anoxic environments, and both DNA and RNA templates, revealed a high percentage of novel 18S rRNA sequences in most phyla, suggesting that marine environments have not yet been fully sampled at a molecular level. This novelty is especially high among Platyhelminthes, Acoelomorpha, and Nematoda, which are well studied from a morphological perspective and abundant in benthic environments. We also identifed, based on molecular data, a potentially novel group of widespread tunicates. Moreover, we recovered a high number of reads for Ctenophora and Cnidaria in the smaller fractions suggesting their gametes might play a greater ecological role than previously suspected. Te animal kingdom is one of the best-studied branches of the tree of life1, with more than 1.5 million species described in around 35 diferent phyla2. -
Tunicata 4 Alberto Stolfi and Federico D
Tunicata 4 Alberto Stolfi and Federico D. Brown Chapter vignette artwork by Brigitte Baldrian. © Brigitte Baldrian and Andreas Wanninger. A. Stolfi Department of Biology , Center for Developmental Genetics, New York University , New York , NY , USA F. D. Brown (*) EvoDevo Laboratory, Departamento de Zoologia , Instituto de Biociências, Universidade de São Paulo , São Paulo , SP , Brazil Evolutionary Developmental Biology Laboratory, Department of Biological Sciences , Universidad de los Andes , Bogotá , Colombia Centro Nacional de Acuicultura e Investigaciones Marinas (CENAIM) , Escuela Superior Politécnica del Litoral (ESPOL) , San Pedro , Santa Elena , Ecuador e-mail: [email protected] A. Wanninger (ed.), Evolutionary Developmental Biology of Invertebrates 6: Deuterostomia 135 DOI 10.1007/978-3-7091-1856-6_4, © Springer-Verlag Wien 2015 [email protected] 136 A. Stolfi and F.D. Brown Above all , perhaps , I am indebted to a decidedly the phylogenetic relationships between the three vegetative , often beautiful , and generally obscure classes and many orders and families have yet to group of marine animals , both for their intrinsic interest and for the enjoyment I have had in search- be satisfactorily settled. Appendicularia, ing for them . N. J. Berrill (1955) Thaliacea, and Ascidiacea remain broadly used in textbooks and scientifi c literature as the three classes of tunicates; however, recent molecular INTRODUCTION phylogenies have provided support for the mono- phyly of only Appendicularia and Thaliacea, but Tunicates are a group of marine fi lter-feeding not of Ascidiacea (Swalla et al. 2000 ; animals1 that have been traditionally divided into Tsagkogeorga et al. 2009 ; Wada 1998 ). A para- three classes: (1) Appendicularia, also known as phyletic Ascidiacea calls for a reevaluation of larvaceans because their free-swimming and tunicate relationships. -
Awesome Ascidians a Guide to the Sea Squirts of New Zealand Version 2, 2016
about this guide | about sea squirts | colour index | species index | species pages | icons | glossary inspirational invertebratesawesome ascidians a guide to the sea squirts of New Zealand Version 2, 2016 Mike Page Michelle Kelly with Blayne Herr 1 about this guide | about sea squirts | colour index | species index | species pages | icons | glossary about this guide Sea squirts are amongst the more common marine invertebrates that inhabit our coasts, our harbours, and the depths of our oceans. AWESOME ASCIDIANS is a fully illustrated e-guide to the sea squirts of New Zealand. It is designed for New Zealanders like you who live near the sea, dive and snorkel, explore our coasts, make a living from it, and for those who educate and are charged with kaitiakitanga, conservation and management of our marine realm. It is one in a series of electronic guides on New Zealand marine invertebrates that NIWA’s Coasts and Oceans centre is presently developing. The e-guide starts with a simple introduction to living sea squirts, followed by a colour index, species index, detailed individual species pages, and finally, icon explanations and a glossary of terms. As new species are discovered and described, new species pages will be added and an updated version of this e-guide will be made available online. Each sea squirt species page illustrates and describes features that enable you to differentiate the species from each other. Species are illustrated with high quality images of the animals in life. As far as possible, we have used characters that can be seen by eye or magnifying glass, and language that is non technical. -
On a Collection of Ascidians from the Southern West Coast of India with Three New Records from Indian Waters
Available online at: www.mbai.org.in doi: 10.6024/jmbai.2015.57.1.01834A-09 On a collection of ascidians from the southern west coast of India with three new records from Indian waters H. Abdul Jaffar Ali*, V. Sivakumar1, M. Tamilselvi2, A. Soban Akram and M. L. Kaleem Arshan Department of Biotechnology, Islamiah College (Autonomous), Vaniyambadi - 635 752, India. 1 Director of Research and Conservation, 4e India NGO (Reg 188/2010). 2Department of Zoology, V.V. Vanniaperumal College for Women, Virudhunagar - 626 001, India. *Correspondence e-mail: [email protected] Received: 30 Dec 2014, Accepted: 09 Apr 2015, Published: 30 Apr 2015 Original Article Abstract Keywords: Ascidian, distribution, diversity, India, southwest Diversity and distribution of 42 species of ascidians belonging to coast, tunicate, new records. 7 families and 19 genera from six different stations along the southwest coast of India were documented. Most of the species were recorded for the first time from the southwest coast of India while three species appear to be new records from India. Previous records of these species in India were usually from Gulf Introduction of Mannar particularly in Thoothukudi water. The family Didemnidae was represented by 13 species of 4 genera followed The Class Ascidiacea of sub-phylum Tunicata constitutes a by Styelidae (11 species of 7 genera), Polyclinidae (7 species of unique group of animals that serve as an essential source 2 genera), Pyuridae (6 species of 2 genera), Perophoridae (two of a variety of studies in fields ranging from development species) and Polycitoridae and Ascidiidae (one species each). The and evolution to immunology and biotechnology. -
Download the Abstract Book In
Cover and back cover designed by Laurel Hiebert – Drawings modified fromLahille, M.F., 1890. Contributions à l’étude anatomique et taxonomique des Tuniciers. Ph.D. Thesis, Fac. Sciences Paris. Toulouse. ORGANIZING COMMITTEE Stefano Tiozzo Rémi Dumollard Alexandre Alié Janet Chenevert Elisabeth Christians Clare Hudson Alex McDougall Hitoyoshi Yasuo WEBSITE AND COMMUNICATION COMMITTEE Faisal Bekkouche Frédéric Bonino Delphine Dauga SCIENTIFIC COMMITTEE Rémi Dumollard (Sorbonne University, CNRS – France) Stefano Tiozzo (Sorbonne University, CNRS – France) Cristian Cañestro (Universitat de Barcelona – Spain) Bo Dong (Ocean University of China – China) Kaoru Imai (Osaka University – Japan) Kazuo Inaba (University of Tsukuba – Japan) Marie Nydam (Centre College – USA) Rosana Rocha (Universidade Federal do Paraná – Brazil) Ute Rothbächer (Universität Innsbruck – Austria) Antonietta Spagnuolo (Stazione Zoologica Anton Dohrn – Italy) Bob Zeller (San Diego State University – USA) CONTENTS GENERAL INFORMATION ..........................................pag. 5 INFORMATION ON THE MEETING .........................pag. 7 PROGRAM AT GLANCE ...............................................pag. 8 PROGRAM ........................................................................pag. 10 TALKS ABSTRACTS ......................................................pag. 15 POSTERS ABSTRACTS .................................................pag. 85 LIST OF PARTICIPANTS ...............................................pag. 151 SPONSORS AND HOST INSTITUTIONS ................pag.