bioRxiv preprint doi: https://doi.org/10.1101/2021.03.04.434001; this version posted March 5, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Zebrafish embryo screen identifies anti-metastasis drugs 2 3 Joji Nakayama1,2,3,4§, Lora Tan1, Boon Cher Goh2, Shu Wang1, 5, Hideki Makinoshima3,6 and 4 Zhiyuan Gong1§ 5 6 1Department of Biological Science, National University of Singapore, Singapore 7 2Cancer Science Institute of Singapore, National University of Singapore, Singapore 8 3Tsuruoka Metabolomics Laboratory, National Cancer Center, Tsuruoka, Japan 9 4Shonai Regional Industry Promotion Center, Tsuruoka, Japan. 10 5Institute of Bioengineering and Nanotechnology, Singapore 11 6Division of Translational Research, Exploratory Oncology Research and Clinical Trial 12 Center, National Cancer Center, Kashiwa, Japan. 13 14 Keywords: Zebrafish, metastasis, gastrulation, phenotyping screening 15 16 §Corresponding authors: 17 Joji Nakayama 18 Tsuruoka Metabolomics Laboratory, National Cancer Center, Mizukami 246-2, Kakuganji, 19 Tsuruoka, Yamagata, Japan 975-0052. 20 email:
[email protected] 21 22 Zhiyuan Gong 23 Department of Biological Science, National University of Singapore, Singapore 117543. 24 email:
[email protected] 25 26 The authors disclose no potential conflicts of interest. 27 Total words excluding abstract, material and methods, references, and figure legends: 1390 28 Number of figures: 2 29 Number of supplementary figures: 3 30 Number of supplementary tables: 3 31 bioRxiv preprint doi: https://doi.org/10.1101/2021.03.04.434001; this version posted March 5, 2021.