DOCSLIB.ORG

WO 2015/100432 A2 2 July 2015 (02.07.2015) P O P CT

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
WO 2015/100432 A2 2 July 2015 (02.07.2015) P O P CT (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2015/100432 A2 2 July 2015 (02.07.2015) P O P CT (51) International Patent Classification: Gerardo, V.; C/o Symbiota, Inc., 1 Memorial Drive, 7th A 63/02 (2006.01) Floor, Cambridge, MA 0242 1 (US). DJONOVIC, Slavica; C/o Symbiota, Inc., 1 Memorial Drive, 7th Floor, Cam (21) International Application Number: bridge, MA 02421 (US). MARQUEZ, Luis, Miguel; C/o PCT/US20 14/072400 Symbiota, Inc., 1 Memorial Drive, 7th Floor, Cambridge, (22) International Filing Date: MA 02421 (US). JOHNSTON, David, Morris; C/o Sym 24 December 2014 (24. 12.2014) biota, Inc., 1 Memorial Drive, 7th Floor, Cambridge, MA 02421 (US). MILLET, Yves, Alain; C/o Symbiota, Inc., 1 (25) Filing Language: English Memorial Drive, 7th Floor, Cambridge, MA 02421 (US). (26) Publication Language: English LYFORD, Jeffrey; C/o Symbiota, Inc., 1 Memorial Drive, 7th Floor, Cambridge, MA 02421 (US). LEFF, Jonathan, (30) Priority Data: W.; C/o Symbiota, Inc., 1 Memorial Drive, 7th Floor, 61/920,560 24 December 201 3 (24. 12.2013) US Cambrdige, MA 02421 (US). SAMAYOA, Philip; C/o 62/017,816 26 June 2014 (26.06.2014) US Symbiota, Inc., 1 Memorial Drive, 7th Floor, Cambridge, 62/017,809 26 June 2014 (26.06.2014) us MA 02421 (US). SADOWSKI, Craig; C/o Symbiota, Inc., PCT/US20 14/044427 26 June 2014 (26.06.2014) us 1 Memorial Drive, 7th Floor, Cambridge, MA 02421 (US). 62/017,813 26 June 2014 (26.06.2014) us 62/017,796 26 June 2014 (26.06.2014) us (74) Agents: SHUSTER, Michael, J. et al; Fenwick & West 14/3 15,804 26 June 2014 (26.06.2014) us LLP, Silicon Valley Center, 801 California Street, Moun 62/017,818 26 June 2014 (26.06.2014) us tain View, CA 94041 (US). 62/017,815 26 June 2014 (26.06.2014) us (81) Designated States (unless otherwise indicated, for every PCT/US20 14/054 160 kind of national protection available): AE, AG, AL, AM, 4 September 2014 (04.09.2014) us AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (71) Applicants: SYMBIOTA, INC. [US/US]; 1 Memorial BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, Drive, 7th Floor, Cambridge, MA 02421 (US). AIT AUS¬ DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, TRIAN INSTITUTE OF TECHNOLOGY GMBH HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, [AT/AT]; Tech Gate Wien Wissensch. U. Tech, Park, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, Donau-cty-str 1, A-1220 Vienna (AT). MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (72) Inventors: MITTER, Birgit; C/o Ait Austrian Institute Of SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, Technology Gmbh, Tech Gate Wien Wissensch. U. Tech. TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Park, Donau-cty-str 1, A-1220 Vienna (AT). NAVEED, Muhammad; C/o Ait Austrian Institute Of Technology (84) Designated States (unless otherwise indicated, for every Gmbh, Tech Gate Wien Wissensch. U. Tech. Park, Donau- kind of regional protection available): ARIPO (BW, GH, cty-str 1, A-1220 Vienna (AT). BERNINGER, Teresa; GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, C/o Ait Austrian Institute Of Technology Gmbh, Tech Gate TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Wien Wissensch. U. Tech. Park, Donau-cty-str 1, A-1200 TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Vienna (AT). COMPANT, Stephane; C/o Ait Austrian In DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, stitute Of Technology Gmbh, Tech Gate Wien Wissensch. LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, U. Tech. Park, Donau-cty-str 1, A-1220 Vienna (AT). SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, SESSITSCH, Angela; C/o Ait Austrian Institute Of Tech GW, KM, ML, MR, NE, SN, TD, TG). nology Gmbh, Tech Gate Wien Wissensch. U. Tech. Park, Published: Donau-cty-str 1, A-1220 Vienna (AT). VON MALTZAHN, Geoffrey; C/o Symbiota, Inc., 1 Memorial — without international search report and to be republished Drive, 7th Floor, Cambridge, MA 02421 (US). FLAV- upon receipt of that report (Rule 48.2(g)) ELL, Richard, Bailey; C/o Sumbiota, Inc., 1 Memorial — with sequence listing part of description (Rule 5.2(a)) Drive, 7th Floor, Cambridge, MA 02421 (US). TOLEDO, < o (54) Title: METHOD FOR PROPAGATING MICROORGANISMS WITHIN PLANT BIOREACTORS AND STABLY STORING o MICROORGANISMS WITHIN AGRICULTURAL SEEDS (57) Abstract: The present invention relates to methods of scalably producing microorganisms by propagating them within plant tis - sues and introducing them into agricultural seeds to improve their shelf-life during long-term storage, to produce substances of in terest, and to create libraries of microbes. METHOD FOR PROPAGATING MICROORGANISMS WITHIN PLANT BIOREACTORS AND STABLY STORING MICROORGANISMS WITHIN AGRICULTURAL SEEDS SEQUENCE LISTING The instant application contains a Sequence Listing which has been filed electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on December 24, 2014, is named 28412PCT_CRF_sequencelisting.txt and is 2,453,823 bytes in size. BACKGROUND Since the biotechnology revolution, there has been a desire to grow a diversity of microbes in low-cost, simple, and scalable culture systems. There has also been a need to generate shelf- stable formulations that can allow low-cost storage of valuable microbes. SUMMARY OF THE INVENTION The present invention relates to methods of scalably producing microorganisms by propagating them within plant tissues and introducing them into agricultural seeds to improve their shelf-life during long-term storage. The present invention is based on the surprising discovery that microbes can be viably incorporated into the seeds of plants by inoculation of various plant tissues. The inventors have discovered that, when a preparation of microorganisms is applied to a plant under select conditions, the microorganisms can gain entry when grain formation starts and establish populations inside, and hence colonize the seed. The methods described herein can be used to introduce new microbes into plants and their seeds as a means of supporting the scalable expansion and storage of the desired microbe. The methods also can produce plants and seeds that uniformly comprise desired microbes and microbial products. These methods can be used to generate plants with valuable microbial constituents that can be difficult to produce with current lab or industrial cultivation methods and can produce seeds comprising microbes in a form that allows the microbe's storage for prolonged periods at room temperature. Also provided are novel compositions of plants, plant parts and seeds containing microbes. In some aspects, disclosed herein is a method of generating a bacterial endophyte library, comprising the steps of providing an inoculum comprising a plurality of bacterial endophyte entities, contacting the inoculum with a cereal plant seed, wherein the cereal plant seed is substantially depleted of surface endophytes, under conditions such that at least two bacterial endophyte entities present in the inoculum are incorporated into a cereal plant grown or derived from the plant seed, such that a bacterial endophyte library is generated within the cereal plant at a concentration of 106 CFU per plant. In certain embodiments, the at least two bacterial endophyte entities are exogenous to the cereal plant seed. In some embodiments, the bacterial endophyte library comprises at least three bacterial endophyte entities. In some embodiments, the bacterial endophyte library comprises at least five bacterial endophyte entities. In some embodiments, the bacterial endophyte library comprises at least ten bacterial endophyte entities. In some embodiments, the bacterial endophyte library comprises at least one bacterial entity not detectably present in the cereal plant seed. In other embodiments, the at least two bacterial endophyte entities comprise a first bacterial endophyte entity exhibiting a first phenotype and a second bacterial endophyte entity exhibiting a second phenotype. In certain embodiments, the first and second phenotypes are selected from catalase activity, oxidase activity, casein hydrolysis activity, gelatin hydrolysis activity, ACC-deaminase activity, exopolysaccharide activity, amylase activity, cellulase activity, chitinase activity, hemolytic activity, lipase activity, pectinase activity, phosphatase activity, protease activity, xylanase activity, production of an auxin, production of an antimicrobial, production of HCN, production of NH3, production of AHL, production of PHB, production of a siderophore, mineral phosphate solubilization, and production of acetoin. In some embodiments, the method further comprises the steps of planting a plurality of the cereal plant seeds and allowing the plants to grow, wherein the bacterial endophyte is present at 10 10 CFU per acre of plants planted according to established agricultural practices. In further embodiments, at least one of the bacterial endophyte entities is capable of using methanol or ethanol as their main carbon source. In some aspects, disclosed herein is a seed preparation comprising the bacterial endophyte library disclosed above, disposed on a plurality of seeds. In other aspects, disclosed herein is a method of producing an endophyte population in a bioreactor, comprising introducing into a bioreactor comprising a cereal plant material at least one bacterial endophyte entity, wherein the bacterial endophyte entity is localized an to an intercellular space of the cereal plant material, under conditions such that the bacterial endophyte entity proliferates within the intercellular space, thereby producing an endophyte population within the bioreactor.
Load more

Recommended publications
  • Metaproteogenomic Insights Beyond Bacterial Response to Naphthalene
    ORIGINAL ARTICLE ISME Journal – Original article Metaproteogenomic insights beyond bacterial response to 5 naphthalene exposure and bio-stimulation María-Eugenia Guazzaroni, Florian-Alexander Herbst, Iván Lores, Javier Tamames, Ana Isabel Peláez, Nieves López-Cortés, María Alcaide, Mercedes V. del Pozo, José María Vieites, Martin von Bergen, José Luis R. Gallego, Rafael Bargiela, Arantxa López-López, Dietmar H. Pieper, Ramón Rosselló-Móra, Jesús Sánchez, Jana Seifert and Manuel Ferrer 10 Supporting Online Material includes Text (Supporting Materials and Methods) Tables S1 to S9 Figures S1 to S7 1 SUPPORTING TEXT Supporting Materials and Methods Soil characterisation Soil pH was measured in a suspension of soil and water (1:2.5) with a glass electrode, and 5 electrical conductivity was measured in the same extract (diluted 1:5). Primary soil characteristics were determined using standard techniques, such as dichromate oxidation (organic matter content), the Kjeldahl method (nitrogen content), the Olsen method (phosphorus content) and a Bernard calcimeter (carbonate content). The Bouyoucos Densimetry method was used to establish textural data. Exchangeable cations (Ca, Mg, K and 10 Na) extracted with 1 M NH 4Cl and exchangeable aluminium extracted with 1 M KCl were determined using atomic absorption/emission spectrophotometry with an AA200 PerkinElmer analyser. The effective cation exchange capacity (ECEC) was calculated as the sum of the values of the last two measurements (sum of the exchangeable cations and the exchangeable Al). Analyses were performed immediately after sampling. 15 Hydrocarbon analysis Extraction (5 g of sample N and Nbs) was performed with dichloromethane:acetone (1:1) using a Soxtherm extraction apparatus (Gerhardt GmbH & Co.
    [Show full text]
  • Major Clades of Agaricales: a Multilocus Phylogenetic Overview
    Mycologia, 98(6), 2006, pp. 982–995. # 2006 by The Mycological Society of America, Lawrence, KS 66044-8897 Major clades of Agaricales: a multilocus phylogenetic overview P. Brandon Matheny1 Duur K. Aanen Judd M. Curtis Laboratory of Genetics, Arboretumlaan 4, 6703 BD, Biology Department, Clark University, 950 Main Street, Wageningen, The Netherlands Worcester, Massachusetts, 01610 Matthew DeNitis Vale´rie Hofstetter 127 Harrington Way, Worcester, Massachusetts 01604 Department of Biology, Box 90338, Duke University, Durham, North Carolina 27708 Graciela M. Daniele Instituto Multidisciplinario de Biologı´a Vegetal, M. Catherine Aime CONICET-Universidad Nacional de Co´rdoba, Casilla USDA-ARS, Systematic Botany and Mycology de Correo 495, 5000 Co´rdoba, Argentina Laboratory, Room 304, Building 011A, 10300 Baltimore Avenue, Beltsville, Maryland 20705-2350 Dennis E. Desjardin Department of Biology, San Francisco State University, Jean-Marc Moncalvo San Francisco, California 94132 Centre for Biodiversity and Conservation Biology, Royal Ontario Museum and Department of Botany, University Bradley R. Kropp of Toronto, Toronto, Ontario, M5S 2C6 Canada Department of Biology, Utah State University, Logan, Utah 84322 Zai-Wei Ge Zhu-Liang Yang Lorelei L. Norvell Kunming Institute of Botany, Chinese Academy of Pacific Northwest Mycology Service, 6720 NW Skyline Sciences, Kunming 650204, P.R. China Boulevard, Portland, Oregon 97229-1309 Jason C. Slot Andrew Parker Biology Department, Clark University, 950 Main Street, 127 Raven Way, Metaline Falls, Washington 99153- Worcester, Massachusetts, 01609 9720 Joseph F. Ammirati Else C. Vellinga University of Washington, Biology Department, Box Department of Plant and Microbial Biology, 111 355325, Seattle, Washington 98195 Koshland Hall, University of California, Berkeley, California 94720-3102 Timothy J.
    [Show full text]
  • Desulfuribacillus Alkaliarsenatis Gen. Nov. Sp. Nov., a Deep-Lineage
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Extremophiles (2012) 16:597–605 DOI 10.1007/s00792-012-0459-7 ORIGINAL PAPER Desulfuribacillus alkaliarsenatis gen. nov. sp. nov., a deep-lineage, obligately anaerobic, dissimilatory sulfur and arsenate-reducing, haloalkaliphilic representative of the order Bacillales from soda lakes D. Y. Sorokin • T. P. Tourova • M. V. Sukhacheva • G. Muyzer Received: 10 February 2012 / Accepted: 3 May 2012 / Published online: 24 May 2012 Ó The Author(s) 2012. This article is published with open access at Springerlink.com Abstract An anaerobic enrichment culture inoculated possible within a pH range from 9 to 10.5 (optimum at pH with a sample of sediments from soda lakes of the Kulunda 10) and a salt concentration at pH 10 from 0.2 to 2 M total Steppe with elemental sulfur as electron acceptor and for- Na? (optimum at 0.6 M). According to the phylogenetic mate as electron donor at pH 10 and moderate salinity analysis, strain AHT28 represents a deep independent inoculated with sediments from soda lakes in Kulunda lineage within the order Bacillales with a maximum of Steppe (Altai, Russia) resulted in the domination of a 90 % 16S rRNA gene similarity to its closest cultured Gram-positive, spore-forming bacterium strain AHT28. representatives. On the basis of its distinct phenotype and The isolate is an obligate anaerobe capable of respiratory phylogeny, the novel haloalkaliphilic anaerobe is suggested growth using elemental sulfur, thiosulfate (incomplete as a new genus and species, Desulfuribacillus alkaliar- T T reduction) and arsenate as electron acceptor with H2, for- senatis (type strain AHT28 = DSM24608 = UNIQEM mate, pyruvate and lactate as electron donor.
    [Show full text]
  • Phylogeny and Classification of the Melastomataceae and Memecylaceae
    Nord. J. Bot. - Section of tropical taxonomy Phylogeny and classification of the Melastomataceae and Memecy laceae Susanne S. Renner Renner, S. S. 1993. Phylogeny and classification of the Melastomataceae and Memecy- laceae. - Nord. J. Bot. 13: 519-540. Copenhagen. ISSN 0107-055X. A systematic analysis of the Melastomataceae, a pantropical family of about 4200- 4500 species in c. 166 genera, and their traditional allies, the Memecylaceae, with c. 430 species in six genera, suggests a phylogeny in which there are two major lineages in the Melastomataceae and a clearly distinct Memecylaceae. Melastomataceae have close affinities with Crypteroniaceae and Lythraceae, while Memecylaceae seem closer to Myrtaceae, all of which were considered as possible outgroups, but sister group relationships in this plexus could not be resolved. Based on an analysis of all morph- ological and anatomical characters useful for higher level grouping in the Melastoma- taceae and Memecylaceae a cladistic analysis of the evolutionary relationships of the tribes of the Melastomataceae was performed, employing part of the ingroup as outgroup. Using 7 of the 21 characters scored for all genera, the maximum parsimony program PAUP in an exhaustive search found four 8-step trees with a consistency index of 0.86. Because of the limited number of characters used and the uncertain monophyly of some of the tribes, however, all presented phylogenetic hypotheses are weak. A synapomorphy of the Memecylaceae is the presence of a dorsal terpenoid-producing connective gland, a synapomorphy of the Melastomataceae is the perfectly acrodro- mous leaf venation. Within the Melastomataceae, a basal monophyletic group consists of the Kibessioideae (Prernandra) characterized by fiber tracheids, radially and axially included phloem, and median-parietal placentation (placentas along the mid-veins of the locule walls).
    [Show full text]
  • Eight New<I> Elaphomyces</I> Species
    VOLUME 7 JUNE 2021 Fungal Systematics and Evolution PAGES 113–131 doi.org/10.3114/fuse.2021.07.06 Eight new Elaphomyces species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America M.A. Castellano1, C.D. Crabtree2, D. Mitchell3, R.A. Healy4 1US Department of Agriculture, Forest Service, Northern Research Station, 3200 Jefferson Way, Corvallis, OR 97331, USA 2Missouri Department of Natural Resources, Division of State Parks, 7850 N. State Highway V, Ash Grove, MO 65604, USA 33198 Midway Road, Belington, WV 26250, USA 4Department of Plant Pathology, University of Florida, Gainesville, FL 32611 USA *Corresponding author: [email protected] Key words: Abstract: The hypogeous, sequestrate ascomycete genus Elaphomyces is one of the oldest known truffle-like genera.Elaphomyces ectomycorrhizae has a long history of consumption by animals in Europe and was formally described by Nees von Esenbeck in 1820 from Europe. hypogeous fungi Until recently most Elaphomyces specimens in North America were assigned names of European taxa due to lack of specialists new taxa working on this group and difficulty of using pre-modern species descriptions. It has recently been discovered that North America sequestrate fungi has a rich diversity of Elaphomyces species far beyond the four Elaphomyces species described from North America prior to 2012. We describe eight new Elaphomyces species (E. dalemurphyi, E. dunlapii, E. holtsii, E. lougehrigii, E. miketroutii, E. roodyi, E. stevemilleri and E. wazhazhensis) of eastern North America that were collected in habitats from Quebec, Canada south to Florida, USA, west to Texas and Iowa. The ranges of these species vary and with continued sampling may prove to be larger than we have established.
    [Show full text]
  • Organic Electron Donors and Terminal Electron Acceptors Structure
    bioRxiv preprint doi: https://doi.org/10.1101/2021.02.16.431432; this version posted February 16, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 Organic electron donors and terminal electron acceptors structure 2 anaerobic microbial communities and interactions in a permanently 3 stratified sulfidic lake 4 Connie A. Rojas1,2, Ana De Santiago Torio3, Serry Park1, Tanja Bosak3*, Vanja Klepac- 5 Ceraj1* 6 1Department of Biological Sciences, Wellesley College, Wellesley, MA, USA. 7 2Ecology, Evolution, and Behavior, Michigan State University, East Lansing, MI, USA. 8 3Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, 9 Cambridge, MA, USA. 10 11 * Correspondence: 12 Tanja Bosak [email protected] 13 Vanja Klepac-Ceraj [email protected] 14 15 Keywords: meromictic lake, microbial community assembly, generalists, enrichment cultures, 16 nutrient cycling, sulfur cycle, carbon cycle, organic electron donors, terminal electron acceptors 17 18 ABstract 19 The extent to which nutrients structure microbial communities in permanently stratified lakes is not 20 well understood. This study characterizeD microbial communities from the anoxic layers of the 21 meromictic and sulfidic Fayetteville Green Lake (FGL), NY, and investigated the roles of organic 22 electron donors and terminal electron acceptors in shaping microbial community structure and 23 interactions. Bacterial communities from the permanently stratified layer below the chemocline 24 (monimolimnion) and from enrichment cultures inoculated by lake sediments were analyzed using 25 16S rRNA gene sequencing.
    [Show full text]
  • Veterinary Toxicology
    GINTARAS DAUNORAS VETERINARY TOXICOLOGY Lecture notes and classes works Study kit for LUHS Veterinary Faculty Foreign Students LSMU LEIDYBOS NAMAI, KAUNAS 2012 Lietuvos sveikatos moksl ų universitetas Veterinarijos akademija Neužkre čiam ųjų lig ų katedra Gintaras Daunoras VETERINARIN Ė TOKSIKOLOGIJA Paskait ų konspektai ir praktikos darb ų aprašai Mokomoji knyga LSMU Veterinarijos fakulteto užsienio studentams LSMU LEIDYBOS NAMAI, KAUNAS 2012 UDK Dau Apsvarstyta: LSMU VA Veterinarijos fakulteto Neužkre čiam ųjų lig ų katedros pos ėdyje, 2012 m. rugs ėjo 20 d., protokolo Nr. 01 LSMU VA Veterinarijos fakulteto tarybos pos ėdyje, 2012 m. rugs ėjo 28 d., protokolo Nr. 08 Recenzavo: doc. dr. Alius Pockevi čius LSMU VA Užkre čiam ųjų lig ų katedra dr. Aidas Grigonis LSMU VA Neužkre čiam ųjų lig ų katedra CONTENTS Introduction ……………………………………………………………………………………… 7 SECTION I. Lecture notes ………………………………………………………………………. 8 1. GENERAL VETERINARY TOXICOLOGY ……….……………………………………….. 8 1.1. Veterinary toxicology aims and tasks ……………………………………………………... 8 1.2. EC and Lithuanian legal documents for hazardous substances and pollution ……………. 11 1.3. Classification of poisons ……………………………………………………………………. 12 1.4. Chemicals classification and labelling ……………………………………………………… 14 2. Toxicokinetics ………………………………………………………………………...………. 15 2.2. Migration of substances through biological membranes …………………………………… 15 2.3. ADME notion ………………………………………………………………………………. 15 2.4. Possibilities of poisons entering into an animal body and methods of absorption ……… 16 2.5. Poison distribution
    [Show full text]
  • Elaphomycetaceae, Eurotiales, Ascomycota) from Africa and Madagascar Indicate That the Current Concept of Elaphomyces Is Polyphyletic
    Cryptogamie, Mycologie, 2016, 37 (1): 3-14 © 2016 Adac. Tous droits réservés Molecular analyses of first collections of Elaphomyces Nees (Elaphomycetaceae, Eurotiales, Ascomycota) from Africa and Madagascar indicate that the current concept of Elaphomyces is polyphyletic Bart BUYCK a*, Kentaro HOSAKA b, Shelly MASI c & Valerie HOFSTETTER d a Muséum national d’Histoire naturelle, département systématique et Évolution, CP 39, ISYEB, UMR 7205 CNRS MNHN UPMC EPHE, 12 rue Buffon, F-75005 Paris, France b Department of Botany, National Museum of Nature and Science (TNS) Tsukuba, Ibaraki 305-0005, Japan, email: [email protected] c Muséum national d’Histoire naturelle, Musée de l’Homme, 17 place Trocadéro F-75116 Paris, France, email: [email protected] d Department of plant protection, Agroscope Changins-Wädenswil research station, ACW, rte de duiller, 1260, Nyon, Switzerland, email: [email protected] Abstract – First collections are reported for Elaphomyces species from Africa and Madagascar. On the basis of an ITS phylogeny, the authors question the monophyletic nature of family Elaphomycetaceae and of the genus Elaphomyces. The objective of this preliminary paper was not to propose a new phylogeny for Elaphomyces, but rather to draw attention to the very high dissimilarity among ITS sequences for Elaphomyces and to the unfortunate choice of species to represent the genus in most previous phylogenetic publications on Elaphomycetaceae and other cleistothecial ascomycetes. Our study highlights the need for examining the monophyly of this family and to verify the systematic status of Pseudotulostoma as a separate genus for stipitate species. Furthermore, there is an urgent need for an in-depth morphological study, combined with molecular sequencing of the studied taxa, to point out the phylogenetically informative characters of the discussed taxa.
    [Show full text]
  • Achromobacter Buckle Infection Diagnosed by a 16S Rdna Clone
    Hotta et al. BMC Ophthalmology 2014, 14:142 http://www.biomedcentral.com/1471-2415/14/142 CASE REPORT Open Access Achromobacter buckle infection diagnosed by a 16S rDNA clone library analysis: a case report Fumika Hotta1†, Hiroshi Eguchi1*, Takeshi Naito1†, Yoshinori Mitamura1†, Kohei Kusujima2† and Tomomi Kuwahara3† Abstract Background: In clinical settings, bacterial infections are usually diagnosed by isolation of colonies after laboratory cultivation followed by species identification with biochemical tests. However, biochemical tests result in misidentification due to similar phenotypes of closely related species. In such cases, 16S rDNA sequence analysis is useful. Herein, we report the first case of an Achromobacter-associated buckle infection that was diagnosed by 16S rDNA sequence analysis. This report highlights the significance of Achromobacter spp. in device-related ophthalmic infections. Case presentation: A 56-year-old woman, who had received buckling surgery using a silicone solid tire for retinal detachment eighteen years prior to this study, presented purulent eye discharge and conjunctival hyperemia in her right eye. Buckle infection was suspected and the buckle material was removed. Isolates from cultures of preoperative discharge and from deposits on the operatively removed buckle material were initially identified as Alcaligenes and Corynebacterium species. However, sequence analysis of a 16S rDNA clone library using the DNA extracted from the deposits on the buckle material demonstrated that all of the 16S rDNA sequences most closely matched those of Achromobacter spp. We concluded that the initial misdiagnosis of this case as an Alcaligenes buckle infection was due to the unreliability of the biochemical test in discriminating Achromobacter and Alcaligenes species due to their close taxonomic positions and similar phenotypes.
    [Show full text]
  • OPENING Remarksâ•Fltwelfth VERTEBRATE PEST CONFERENCE
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Proceedings of the Twelfth Vertebrate Pest Vertebrate Pest Conference Proceedings Conference (1986) collection March 1986 OPENING REMARKS—TWELFTH VERTEBRATE PEST CONFERENCE Terrell P. Salmon University of California, Davis, California Follow this and additional works at: https://digitalcommons.unl.edu/vpc12 Part of the Environmental Health and Protection Commons Salmon, Terrell P., "OPENING REMARKS—TWELFTH VERTEBRATE PEST CONFERENCE" (1986). Proceedings of the Twelfth Vertebrate Pest Conference (1986). 1. https://digitalcommons.unl.edu/vpc12/1 This Article is brought to you for free and open access by the Vertebrate Pest Conference Proceedings collection at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Proceedings of the Twelfth Vertebrate Pest Conference (1986) by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. OPENING REMARKS—TWELFTH VERTEBRATE PEST CONFERENCE TERRELL P. SALMON, Wildlife Specialist, Cooperative Extension, University of California, Davis, California 95616. On behalf of the Vertebrate Pest Council, welcome to the 12th Vertebrate Pest Conference. Every other year since 1962, the Vertebrate Pest Council has sponsored the conference with the primary objective of bringing individuals interested in vertebrate pest control together to discuss problems and solutions of mutual concern. The main objectives of the conference are: 1. To exchange information on vertebrate pest management and related matters. 2. To advance environmentally safe vertebrate pest management methodologies. 3. To build cooperation with public and private agencies in solving vertebrate pest problems. 4. To consider and promote discussion and interaction among agencies and others about problems of mutual concern in the field of vertebrate pest management.
    [Show full text]
  • Gut Microbiome Alterations in Ulcerative Colitis and After Moxibustion Intervention
    Gut Microbiome Alterations In Ulcerative Colitis And After Moxibustion Intervention Qin Qi Shanghai University of Traditional Chinese Medicine Ya-Nan Liu Shanghai University of Traditional Chinese Medicine Si-Yi Lv Shanghai University of Traditional Chinese Medicine Huan-Gan Wu Shanghai University of Traditional Chinese Medicine Lin-Shuang Zhang Zhejiang Institute for Food and Drug Control Zhan Cao Tongji University School of Medicine Hui-Rong Liu Shanghai University of Traditional Chinese Medicine Xiao-Mei Wang ( [email protected] ) Shanghai University of Traditional Chinese Medicine Lu-Yi Wu Shanghai University of Traditional Chinese Medicine Research Article Keywords: Ulcerative colitis, Moxibustion, Gut microbiota, Metagenomic Posted Date: August 11th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-789670/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/22 Abstract Background: Recent studies have shown that the pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota. Moxibustion, a common treatment in traditional Chinese medicine, is the burning of the herb moxa over acupuncture points. Moxibustion has been used to improve the inammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC. In this study, we investigated whether moxibustion could improve the gut microbial dysbiosis induced by dextran sulphate sodium (DSS). Methods: Twenty-ve male rats were randomly assigned into ve groups: normal (NG), UC model (UC), moxibustion (UC+MOX), mesalazine (UC+MES), and normal rats with moxibustion (NG+MOX). The UC rat model was established by administering DSS solution. The rats in the UC+MOX and NG+MOX groups were treated with moxibustion at Tianshu (bilateral, ST25) points once daily for 7 consecutive days, and the UC+MES group rats were treated with mesalazine once daily for 7 consecutive days.
    [Show full text]
  • The Veterinarian's Guide
    The Veterinarian’s Guide Bromethalin Addendum The Soft Bait Innovators™ Liphatech, Inc. • 3600 W. Elm Street • Milwaukee, WI 53209 • 1-888-331-7900 • www.liphatech.com NOTE: The information in this guide does not represent labeling and does not replace information on rodenticide labels relating to exposure of non-target species. Please read and follow all label directions on all rodenticide products you are using. Mentions of trade names in this publication does not imply endorsement of these products. Assault® is a registered trademark of PM Resources (St. Louis, MO) TakeDown™ and Cannon™ are trademarks of Liphatech, Inc. (Milwaukee, WI) Gunslinger® is a registered trademark of Liphatech, Inc. (Milwaukee, WI) Fastrac® is a registered trademark of Bell Labs (Madison, WI) Top Gun™ is a trademark of JT Eaton (Twinsburg, OH) Tomcat® and Rampage® are registered trademarks of Motomco (Madison, WI) Trounce® is a registered trademark of Agricultural Feeds (St. Louis, MO) At the time of this printing all information was deemed accurate and reliable. Liphatech, Inc. 3600 W. Elm Street Milwaukee, WI 53209 1-888-331-7900 www.liphatech.com October, 2016 The Veterinarian’s Guide - Bromethalin Addendum The Veterinarian’s Guide to Accidental Rodenticide Ingestion by Dogs and Cats focuses on anticoagulants. This addendum is intended to help veterinarians recognize and treat the symptoms of bromethalin toxicity in domestic animals. It describes bromethalin’s mode of action and how it is used as a rodenticide. In addition to reviewing the symptoms of bromethalin poisoning in domestic animals and the acute toxicity levels for dogs and cats (Table 5), we have also included the American Society for Prevention of Cruelty to Animals’ Animal Poison Control Center (ASPCA APCC) recommendations for decontamination (Tables 6 and 7).
    [Show full text]