Biodiv. Res. Conserv. 19: 23-32, 2010 BRC www.brc.amu.edu.pl 10.2478/v10119-010-0006-2 Genetic relationships between some of Malva species as determined with ISSR and ISJ markers Zbigniew Celka1, Monika SzczeciÒska2 & Jakub Sawicki2 1Department of Plant Taxonomy, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 PoznaÒ, Poland, e-mail:
[email protected] 2Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac £Ûdzki 1, 10-727 Olsztyn, Poland, e-mail:
[email protected],
[email protected] Abstract: Two categories of DNA markers were used to determine genetic relationships among eight Malva taxa. A maxi- mum parsimony analysis validated the division of the genus Malva into the sections Bismalva and Malva. The species classi- fied into those sections formed separate clusters. M. moschata was a distinctive species in the section Bismalva, as confirmed by previous genetic research based on ITS and cpDNA sequence analyses. The applied markers revealed a very high level of genetic identity between M. alcea and M. excisa and enabled molecular identification of M. alcea var. fastigiata. Species- specific markers were determined for the majority of the analyzed species, permitting their molecular identification. A specific marker supporting the differentiation of M. alcea and M. excisa was not found. Key words: Malva, genetic similarity, molecular markers, ISJ, ISSR 1. Introduction Species of the genus Malva receive wide coverage in scientific papers investigating variations in their The genus Malva comprises around 40 species seeds, seed coats (Celka et al. 2006a; Kumar & Dalbir world-wide (Mabberley 1987), including 13 species Singh 1991), pollen grains (El Naggar 2004) and stem occurring in Europe (Dalby 1968).