Genome Sequence Analysis of First Ross River Virus Isolate from Papua New Guinea Indicates Long-Term, Local Evolution

Total Page:16

File Type:pdf, Size:1020Kb

Genome Sequence Analysis of First Ross River Virus Isolate from Papua New Guinea Indicates Long-Term, Local Evolution viruses Communication Genome Sequence Analysis of First Ross River Virus Isolate from Papua New Guinea Indicates Long-Term, Local Evolution Alice Michie 1, John S. Mackenzie 2,3,4 , David W. Smith 2 and Allison Imrie 1,* 1 School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia; [email protected] 2 PathWest Laboratory Medicine Western Australia, Nedlands, WA 6009, Australia; [email protected] (J.S.M.); [email protected] (D.W.S.) 3 School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD 4072, Australia 4 Faculty of Health Sciences, Curtin University, Bentley, WA 6102, Australia * Correspondence: [email protected] Abstract: Ross River virus (RRV) is the most medically significant mosquito-borne virus of Australia, in terms of human morbidity. RRV cases, characterised by febrile illness and potentially persistent arthralgia, have been reported from all Australian states and territories. RRV was the cause of a large- scale epidemic of multiple Pacific Island countries and territories (PICTs) from 1979 to 1980, involving at least 50,000 cases. Historical evidence of RRV seropositivity beyond Australia, in populations of Papua New Guinea (PNG), Indonesia and the Solomon Islands, has been documented. We describe the genomic characterisation and timescale analysis of the first isolate of RRV to be sampled from PNG to date. Our analysis indicates that RRV has evolved locally within PNG, independent of Australian lineages, over an approximate 40 year period. The mean time to most recent common Citation: Michie, A.; Mackenzie, J.S.; ancestor (tMRCA) of the unique PNG clade coincides with the initiation of the PICTs epidemic in Smith, D.W.; Imrie, A. Genome mid-1979. This may indicate that an ancestral variant of the PNG clade was seeded into the region Sequence Analysis of First Ross River Virus Isolate from Papua New Guinea during the epidemic, a period of high RRV transmission. Further epidemiological and molecular- Indicates Long-Term, Local Evolution. based surveillance is required in PNG to better understand the molecular epidemiology of RRV in Viruses 2021, 13, 482. https:// the general Australasian region. doi.org/10.3390/v13030482 Keywords: arbovirus; mosquito-borne disease; alphavirus; Papua New Guinea; Australia Academic Editors: Bas B. Oude Munnink, Marietjie Venter and Luisa Barzon 1. Introduction Received: 15 February 2021 In Australia, the most common mosquito-borne viral infection is caused by Ross Accepted: 12 March 2021 River virus (RRV), an alphavirus of the family Togaviridae [1]. Disease associated with RRV Published: 15 March 2021 infection is characterised by potentially persistent and debilitating fatigue, myalgia, and arthralgia and/or arthritis. RRV cases have been reported from all Australian states and Publisher’s Note: MDPI stays neutral territories since it was first isolated from Aedes vigilax mosquitoes sampled in far-north with regard to jurisdictional claims in Queensland in 1959 [2]. published maps and institutional affil- iations. RRV is currently considered to be both a mosquito vector species and amplifying host generalist, with macropods (such as wallabies and kangaroos) considered the major reservoir host for RRV maintenance and transmission [3]. Humans have been implicated as short-term amplifying hosts during disease epidemics including the large-scale, virgin-soil epidemic that occurred across multiple Pacific Island countries and territories (PICTs) from Copyright: © 2021 by the authors. 1979 to 1980 [4–6]. Cases were reported from Fiji, American Samoa, the Cook Islands, Licensee MDPI, Basel, Switzerland. New Caledonia and Wallis and Futuna, with disease suspected in northern Tonga [7]. This article is an open access article Samoa did not report cases during this time, but during an April 1980 investigation of a distributed under the terms and conditions of the Creative Commons dengue outbreak, high titres of anti-RRV haemagglutination inhibition (HI) antibody were Attribution (CC BY) license (https:// detected. RRV activity likely occurred in Samoa during the 1979–1980 outbreak but went creativecommons.org/licenses/by/ undetected [7]. Viraemic travelers are the suspected mode of rapid spread between these 4.0/). adjacent island nations [8]. Viruses 2021, 13, 482. https://doi.org/10.3390/v13030482 https://www.mdpi.com/journal/viruses Viruses 2021, 13, 482 2 of 8 RRV transmission seemingly ceased in the region in mid-1980, presumably due to the lack of efficient reservoir hosts in the area [7,8]. A viraemic traveler from eastern Australia has been postulated as the source of the outbreak, which began in Fiji in April 1979, based on sequence similarity of epidemic viruses to the ‘Eastern-Australian’ lineage of RRV [9]. The geographical basis of RRV lineage nomenclature was not supported in our recent, more comprehensive genome-scale RRV phylogenetic analysis, where we showed that virus isolates that clustered as distinct lineages were geographically dispersed and sampled Australia wide [10]. The exact origins of the PICTs epidemic is now less certain, given viruses similar to those sampled during the epidemic, were sampled across Australia between 1982 and 1988 [10]. Four distinct genotypes of RRV (G1–4) have been characterised, with G4 in contemporary circulation across Australia [10]. RRV seropositivity has been described in the adult and general population of many regions of Papua New Guinea (PNG), sampled from 1960 to 1969, as well as regions of In- donesia and the Solomon Islands [11]. A later study found a 59% RRV seroprevalence in the Southern Highlands of PNG in 1991, and endemicity was suggested by increasing antibody prevalence with age [12]. RRV as a cause of arthritis in PNG has been confirmed [13,14]. RRV has been isolated from several mosquito species within Australia that are also present in PNG including Ae. vigilax, Ae. notoscriptus and Mansonia uniformis [15,16]. The exact prevalence, distribution, and genetic diversity of RRV in PNG is currently unknown, due to the lack of comprehensive surveillance in the region [17]. RRV cases were not reported from PNG during the PICTs epidemic. The first and only isolation of RRV from PNG to date was made in 1997 from a pool of Anopheles farauti mosquitoes collected at Wandoo village in the Bensbach region of the Western Province [16]. In this study, the genome sequence of this PNG isolate was derived and analysed with available RRV genome sequences to better understand the molecular epidemiology of RRV in the greater Australasian region. 2. Materials and Methods 2.1. Virus Isolation, RNA Extraction and Next Generation Sequencing The virus isolate, PNG3075, was isolated from a pool of An. farauti mosquitoes trapped in 1997 from the Bensbach locality of the Western Province of PNG [16]. The isolate was identified as RRV using a tissue culture-based fixed cell enzyme immunoassay involving specific, identifying monoclonal antibodies on fixed C6/36 monolayers (Ae. albopictus larvae, ATCC® CRL-1660™)[16,18]. The isolate was not investigated on a molecular level at the time of its initial characterisation. PNG3075 was amplified in the present study through a single passage on Vero cells (African Green Monkey kidney epithelium, ATCC® CCL-81™) maintained in Dulbecco’s Modified Eagle Media (Gibco DMEM, Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented (by volume) with 5% heat-inactivated fetal bovine serum (FBS), 1% L- glutamine (Life Technologies, Carlsbad, California, USA) and 1% penicillin/streptomycin (Life Technologies). The Roche High Pure RNA extraction kit (Roche, Basal, Switzer- land) was utilised as per the manufacturers protocol from viral supernatant, concentrated through ultra-centrifugation in Millipore Amicon Ultra-15 centrifugal units (Merck Milli- pore, Darmstadt, Germany). The TruSeq stranded mRNA kit (Illumina, San Diego, California, USA) was used for library preparation. Superscript III reverse transcriptase (Invitrogen, Waltham, Mas- sachusetts, USA) was used for cDNA synthesis. Libraries were validated using the Agilent 1000 DNA kit (Agilent Technologies, Santa Clara, California, USA) and subsequently nor- malized and pooled. Sequence data were generated on the MiSeq platform with the MiSeq Micro v2 kit (Illumina). Paired-end reads of 150 bp were demultiplexed and assessed for quality within the FastQC v0.11 program [19], and subject to de novo genome sequence generation within CLC Genomics Workbench v7.5 (QIAGEN, Hilden, Germany) to derive a full-coding contiguous sequence, 11,837 nucleotides in length. A total of 567,724 reads Viruses 2021, 13, 482 3 of 8 were generated for the PNG3075 sample, of which 86.89% (493,294 reads) were used to generate the complete coding PNG3075 sequence. 2.2. Phylogenetic Analysis The derived genome was aligned with 104 publicly available RRV genome sequences using MAFFT 7.338 within Geneious 11.1.4 [20]. Most available sequence data were derived in our recent RRV phylogenetic investigation, with isolates primarily sampled in Western Australia [10]. For phylogenetic analysis, sequences were manually trimmed to remove 50 and 30 untranslated regions (UTRs) and then realigned. A maximum likelihood phylogeny was reconstructed using RAxML 8.2.11 with 1000 bootstrap replicates within Geneious 11.1.4, under a GTR+G+I nucleotide substitution model—the best-suited model according to JModelTest 2 analysis [21,22]. All phylogenies were visualized and edited within FigTree v1.4.4 [23]. 2.3. Temporal
Recommended publications
  • Morphology and Protein Profiles of Salivary Glands of Filarial Vector Mosquito Mansonia Uniformis; Possible Relation to Blood Feeding Process
    Asian Biomedicine Vol. 5 No. 3 June 2011; 353-360 DOI: 10.5372/1905-7415.0502.046 Original article Morphology and protein profiles of salivary glands of filarial vector mosquito Mansonia uniformis; possible relation to blood feeding process Atchara Phumeea, Kanok Preativatanyoub, Kanyarat Kraivichainb, Usavadee Thavarac, Apiwat Tawatsinc, Yutthana Phusupc, Padet Siriyasatienb aMedical Science Program, bDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330; cNational Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand Background: Vector control is a key strategy for eradication of filariasis, but it is limited, possibly due to rapid propagation from global warming. In Thailand, Mansonia mosquitoes are major vectors of filariasis caused by Brugia malayi filarial nematodes. However, little is yet known about vector biology and host-parasite relationship. Objectives: Demonstrate the preliminary data of salivary gland morphology and protein profile of human filarial mosquitoes M. uniformis. Methods: Morphology of M. uniformis salivary gland in both sexes was comparatively studied under a light microscope. Total protein quantization and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS- PAGE) was performed to compare protein profile between male and female. In addition, quantitative analysis prior to and after blood feeding was made at different times (0, 12, 24, 36, 48, 60, and 72 hours) Results: Total salivary gland protein of males and females was 0.32±0.03 and 1.38±0.02 μg/pair gland, respectively. SDS-PAGE analysis of the female salivary gland protein prior to blood meal demonstrated twelve bands of major proteins at 21, 22, 24, 26, 37, 39, 44, 53, 55, 61, 72, and 100 kDa.
    [Show full text]
  • Mosquitoes in DENGUE MOSQUITOES the World? ARE MOST ACTIVE DURING BO the DAY AROUND a U S T YOUR YARD T SALTMARSH MOSQUITOES C ARE MOST a ACTIVE at DUSK
    Mosquito awareness Did you know... Mosquito species there are 3500 vary in their species of biting behaviour mosquitoes in DENGUE MOSQUITOES the world? ARE MOST ACTIVE DURING BO THE DAY AROUND A U S T YOUR YARD T SALTMARSH MOSQUITOES C ARE MOST A ACTIVE AT DUSK AND DAWN F Council conducts 300 SPECIES IN AUSTRALIA mosquito control 40 SPECIES IN TOWNSVILLE World's COUNCIL CONDUCTS deadliest MOSQUITO CONTROL ON MOSQUITOES PUBLIC LAND, USING BOTH animals GROUND AND AERIAL TREATMENTS TO TARGET NUMBER OF PEOPLE MOSQUITO LARVAE. KILLED BY ANIMALS PER YEAR Mosquitoes wings beat 300-600 times per second Mosquitoes Mosquitoes can carry are attracted many diseases. to humans FROM THE ODOURS AND CARBON DIOXIDE WE EXPIRE FROM BREATHING Protect yourself OR SWEATING. townsville.qld.gov.au and your family Mosquitoes distance of travel 13 48 10 from mosquito bites from breeding point by using personal DENGUE MOSQUITO SALTMARSH MOSQUITO protection. 200M 50KM BREEDING PLACE Mosquito Mosquito Mosquito life cycle disease prevention A mosquito is an insect characterised by Protect yourself Did you know... Dengue. 1. Three body parts against disease-carrying Townsville City Do your weekly a. Head mosquitoes Council undertakes yard check. b. Thorax c. Abdomen reactive inspection ARE YOU MAKING DENGUE Mosquito borne How do of properties within MOSSIES WELCOME 2. A proboscis (for AROUND YOUR HOME? piercing and sucking) diseases found in mosquitoes the Townsville local TAKE RESPONSIBILITY TO 3. One pair of antennae Townsville include transmit government area PROTECT YOURSELF AND 4. One pair of wings YOUR FAMILY BY CHECKING Ross River virus diseases? based on customer YOUR YARD FOR ANYTHING 5.
    [Show full text]
  • California Encephalitis Orthobunyaviruses in Northern Europe
    California encephalitis orthobunyaviruses in northern Europe NIINA PUTKURI Department of Virology Faculty of Medicine, University of Helsinki Doctoral Program in Biomedicine Doctoral School in Health Sciences Academic Dissertation To be presented for public examination with the permission of the Faculty of Medicine, University of Helsinki, in lecture hall 13 at the Main Building, Fabianinkatu 33, Helsinki, 23rd September 2016 at 12 noon. Helsinki 2016 Supervisors Professor Olli Vapalahti Department of Virology and Veterinary Biosciences, Faculty of Medicine and Veterinary Medicine, University of Helsinki and Department of Virology and Immunology, Hospital District of Helsinki and Uusimaa, Helsinki, Finland Professor Antti Vaheri Department of Virology, Faculty of Medicine, University of Helsinki, Helsinki, Finland Reviewers Docent Heli Harvala Simmonds Unit for Laboratory surveillance of vaccine preventable diseases, Public Health Agency of Sweden, Solna, Sweden and European Programme for Public Health Microbiology Training (EUPHEM), European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden Docent Pamela Österlund Viral Infections Unit, National Institute for Health and Welfare, Helsinki, Finland Offical Opponent Professor Jonas Schmidt-Chanasit Bernhard Nocht Institute for Tropical Medicine WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research National Reference Centre for Tropical Infectious Disease Hamburg, Germany ISBN 978-951-51-2399-2 (PRINT) ISBN 978-951-51-2400-5 (PDF, available
    [Show full text]
  • The Non-Human Reservoirs of Ross River Virus: a Systematic Review of the Evidence Eloise B
    Stephenson et al. Parasites & Vectors (2018) 11:188 https://doi.org/10.1186/s13071-018-2733-8 REVIEW Open Access The non-human reservoirs of Ross River virus: a systematic review of the evidence Eloise B. Stephenson1*, Alison J. Peel1, Simon A. Reid2, Cassie C. Jansen3,4 and Hamish McCallum1 Abstract: Understanding the non-human reservoirs of zoonotic pathogens is critical for effective disease control, but identifying the relative contributions of the various reservoirs of multi-host pathogens is challenging. For Ross River virus (RRV), knowledge of the transmission dynamics, in particular the role of non-human species, is important. In Australia, RRV accounts for the highest number of human mosquito-borne virus infections. The long held dogma that marsupials are better reservoirs than placental mammals, which are better reservoirs than birds, deserves critical review. We present a review of 50 years of evidence on non-human reservoirs of RRV, which includes experimental infection studies, virus isolation studies and serosurveys. We find that whilst marsupials are competent reservoirs of RRV, there is potential for placental mammals and birds to contribute to transmission dynamics. However, the role of these animals as reservoirs of RRV remains unclear due to fragmented evidence and sampling bias. Future investigations of RRV reservoirs should focus on quantifying complex transmission dynamics across environments. Keywords: Amplifier, Experimental infection, Serology, Virus isolation, Host, Vector-borne disease, Arbovirus Background transmission dynamics among arboviruses has resulted in Vertebrate reservoir hosts multiple definitions for the key term “reservoir” [9]. Given Globally, most pathogens of medical and veterinary im- the diversity of virus-vector-vertebrate host interactions, portance can infect multiple host species [1].
    [Show full text]
  • A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus B
    viruses Article A New Orbivirus Isolated from Mosquitoes in North-Western Australia Shows Antigenic and Genetic Similarity to Corriparta Virus but Does Not Replicate in Vertebrate Cells Jessica J. Harrison 1,†, David Warrilow 2,†, Breeanna J. McLean 1, Daniel Watterson 1, Caitlin A. O’Brien 1, Agathe M.G. Colmant 1, Cheryl A. Johansen 3, Ross T. Barnard 1, Sonja Hall-Mendelin 2, Steven S. Davis 4, Roy A. Hall 1 and Jody Hobson-Peters 1,* 1 Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Australia; [email protected] (J.J.H.); [email protected] (B.J.M.); [email protected] (D.W.); [email protected] (C.A.O.B.); [email protected] (A.M.G.C.); [email protected] (R.T.B.); [email protected] (R.A.H.) 2 Public Health Virology Laboratory, Department of Health, Queensland Government, P.O. Box 594, Archerfield 4108, Australia; [email protected] (D.W.); [email protected] (S.H.-M.) 3 School of Pathology and Laboratory Medicine, The University of Western Australia, Nedlands 6009, Australia; [email protected] 4 Berrimah Veterinary Laboratory, Department of Primary Industries and Fisheries, Darwin 0828, Australia; [email protected] * Correspondence: [email protected]; Tel.: +61-7-3365-4648 † These authors contributed equally to the work. Academic Editor: Karyn Johnson Received: 19 February 2016; Accepted: 10 May 2016; Published: 20 May 2016 Abstract: The discovery and characterisation of new mosquito-borne viruses provides valuable information on the biodiversity of vector-borne viruses and important insights into their evolution.
    [Show full text]
  • Table 1 Comprehensive International Points List
    Table 1 Comprehensive International Points List FCC ITU-T Country Region Dialing FIPS Comments, including other 1 Code Plan Code names commonly used Abu Dhabi 5 971 TC include with United Arab Emirates Aden 5 967 YE include with Yemen Admiralty Islands 7 675 PP include with Papua New Guinea (Bismarck Arch'p'go.) Afars and Assas 1 253 DJ Report as 'Djibouti' Afghanistan 2 93 AF Ajman 5 971 TC include with United Arab Emirates Akrotiri Sovereign Base Area 9 44 AX include with United Kingdom Al Fujayrah 5 971 TC include with United Arab Emirates Aland 9 358 FI Report as 'Finland' Albania 4 355 AL Alderney 9 44 GK Guernsey (Channel Islands) Algeria 1 213 AG Almahrah 5 967 YE include with Yemen Andaman Islands 2 91 IN include with India Andorra 9 376 AN Anegada Islands 3 1 VI include with Virgin Islands, British Angola 1 244 AO Anguilla 3 1 AV Dependent territory of United Kingdom Antarctica 10 672 AY Includes Scott & Casey U.S. bases Antigua 3 1 AC Report as 'Antigua and Barbuda' Antigua and Barbuda 3 1 AC Antipodes Islands 7 64 NZ include with New Zealand Argentina 8 54 AR Armenia 4 374 AM Aruba 3 297 AA Part of the Netherlands realm Ascension Island 1 247 SH Ashmore and Cartier Islands 7 61 AT include with Australia Atafu Atoll 7 690 TL include with New Zealand (Tokelau) Auckland Islands 7 64 NZ include with New Zealand Australia 7 61 AS Australian External Territories 7 672 AS include with Australia Austria 9 43 AU Azerbaijan 4 994 AJ Azores 9 351 PO include with Portugal Bahamas, The 3 1 BF Bahrain 5 973 BA Balearic Islands 9 34 SP include
    [Show full text]
  • Sequencing of Historical Isolates, K-Mer Mining and High Serological Cross-Reactivity with Ross River Virus Argue Against the Presence of Getah Virus in Australia
    pathogens Article Sequencing of Historical Isolates, K-mer Mining and High Serological Cross-Reactivity with Ross River Virus Argue against the Presence of Getah Virus in Australia 1, 1, 1 2 3 Daniel J. Rawle y , Wilson Nguyen y , Troy Dumenil , Rhys Parry , David Warrilow , Bing Tang 1, Thuy T. Le 1, Andrii Slonchak 2, Alexander A. Khromykh 2,4, Viviana P. Lutzky 1, Kexin Yan 1 and Andreas Suhrbier 1,4,* 1 Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia; [email protected] (D.J.R.); [email protected] (W.N.); [email protected] (T.D.); [email protected] (B.T.); [email protected] (T.T.L.); [email protected] (V.P.L.); [email protected] (K.Y.) 2 School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia; [email protected] (R.P.); [email protected] (A.S.); [email protected] (A.A.K.) 3 Public Health Virology Laboratory, Department of Health, Queensland Government, Brisbane, QLD 4108, Australia; [email protected] 4 GVN Center of Excellence, Australian Infectious Diseases Research Centre, Brisbane, QLD 4006 and 4072, Australia * Correspondence: [email protected] These authors equally contributed to this work. y Received: 14 September 2020; Accepted: 15 October 2020; Published: 16 October 2020 Abstract: Getah virus (GETV) is a mosquito-transmitted alphavirus primarily associated with disease in horses and pigs in Asia. GETV was also reported to have been isolated from mosquitoes in Australia in 1961; however, retrieval and sequencing of the original isolates (N544 and N554), illustrated that these viruses were virtually identical to the 1955 GETVMM2021 isolate from Malaysia.
    [Show full text]
  • Ants (Hymenoptera: Fonnicidae) of Samoa!
    Ants (Hymenoptera: Fonnicidae) of Samoa! James K Wetterer 2 and Donald L. Vargo 3 Abstract: The ants of Samoa have been well studied compared with those of other Pacific island groups. Using Wilson and Taylor's (1967) specimen records and taxonomic analyses and Wilson and Hunt's (1967) list of 61 ant species with reliable records from Samoa as a starting point, we added published, unpublished, and new records ofants collected in Samoa and updated taxonomy. We increased the list of ants from Samoa to 68 species. Of these 68 ant species, 12 species are known only from Samoa or from Samoa and one neighboring island group, 30 species appear to be broader-ranged Pacific natives, and 26 appear to be exotic to the Pacific region. The seven-species increase in the Samoan ant list resulted from the split of Pacific Tetramorium guineense into the exotic T. bicarinatum and the native T. insolens, new records of four exotic species (Cardiocondyla obscurior, Hypoponera opaciceps, Solenopsis geminata, and Tetramorium lanuginosum), and new records of two species of uncertain status (Tetramorium cf. grassii, tentatively considered a native Pacific species, and Monomorium sp., tentatively considered an endemic Samoan form). SAMOA IS AN ISLAND CHAIN in western island groups, prompting Wilson and Taylor Polynesia with nine inhabited islands and (1967 :4) to feel "confident that a nearly numerous smaller, uninhabited islands. The complete faunal list could be made for the western four inhabited islands, Savai'i, Apo­ Samoan Islands." Samoa is of particular in­ lima, Manono, and 'Upolu, are part of the terest because it is one of the easternmost independent country of Samoa (formerly Pacific island groups with a substantial en­ Western Samoa).
    [Show full text]
  • Clinically Important Vector-Borne Diseases of Europe
    Natalie Cleton, DVM Erasmus MC, Rotterdam Department of Viroscience [email protected] No potential conflicts of interest to disclose © by author ESCMID Online Lecture Library Erasmus Medical Centre Department of Viroscience Laboratory Diagnosis of Arboviruses © by author Natalie Cleton ESCMID Online LectureMarion Library Koopmans Chantal Reusken [email protected] Distribution Arboviruses with public health impact have a global and ever changing distribution © by author ESCMID Online Lecture Library Notifications of vector-borne diseases in the last 6 months on Healthmap.org Syndromes of arboviral diseases 1) Febrile syndrome: – Fever & Malaise – Headache & retro-orbital pain – Myalgia 2) Neurological syndrome: – Meningitis, encephalitis & myelitis – Convulsions & coma – Paralysis 3) Hemorrhagic syndrome: – Low platelet count, liver enlargement – Petechiae © by author – Spontaneous or persistent bleeding – Shock 4) Arthralgia,ESCMID Arthritis and Online Rash: Lecture Library – Exanthema or maculopapular rash – Polyarthralgia & polyarthritis Human arboviruses: 4 main virus families Family Genus Species examples Flaviviridae flavivirus Dengue 1-5 (DENV) West Nile virus (WNV) Yellow fever virus (YFV) Zika virus (ZIKV) Tick-borne encephalitis virus (TBEV) Togaviridae alphavirus Chikungunya virus (CHIKV) O’Nyong Nyong virus (ONNV) Mayaro virus (MAYV) Sindbis virus (SINV) Ross River virus (RRV) Barmah forest virus (BFV) Bunyaviridae nairo-, phlebo-©, orthobunyavirus by authorCrimean -Congo heamoragic fever (CCHFV) Sandfly fever virus
    [Show full text]
  • Mosquitoes Sampling Strategy for Studying West Nile Virus Vectors In
    Sébastien Boyer et al., Archives de l’Institut Pasteur de Madagascar 2014; 71 (1) : 1-8 Article original Mosquitoes sampling strategy for studying West Nile Virus Vectors in Madagascar: Abundance, Distribution and Methods of Catching in High Risk Areas Sébastien Boyer1,*, Michael Luciano Tantely1, Sanjiarizaha Randriamaherijaona1, Lala Andrianaivolambo1, Eric Cardinale 2,3,4 1 Laboratoire d’Entomologie Médicale, Institut Pasteur de Madagascar, Antananarivo, Madagascar 2 Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 15 CMAEE, F-97490 Sainte Clotilde, La Réunion, France 3 Institut National de la Recherche Agronomique (INRA), UMR 1309 CMAEE, F-97490 Sainte Clotilde, La Réunion, France 4 Centre de Recherche et de Veille sur les maladies émergentes dans l’Océan Indien (CRVOI), plateforme de recherche CYROI, F-97490 Sainte Clotilde, La Réunion, France * Corresponding author: [email protected] ABSTRACT The West Nile Virus (WNV) is a mosquito-borne virus discovered in 1937, and first described in 1978 in Madagascar. Twenty-six potential mosquito-vector species mainly ornithophilic were described in Madagascar. Investigations on catching methods of mosquitoes vectors of WNV were carried out in two districts located in the Malagasy west coast where high prevalence was detected in 2009 after a serological survey. Five different methods were evaluated during the samplings: CDC light traps and net-trap baited were tested in Mitsinjo district, while human landing catch, CDC light trap, and BioGent (BG) sentinel were used in Masoarivo. One thousand five hundred eleven adult mosquitoes were collected with between 53% and 66% of them captured by CDC light traps in the two districts.
    [Show full text]
  • Migration Data in Oceania Opening Remarks Global Migration Data Portal* • Challenge: Migration Data Are Scattered Between Various Institutions and Countries
    Migration Data in Oceania Opening Remarks Global Migration Data Portal* • Challenge: Migration data are scattered between various institutions and countries • Proposed on 12 July 2016 by then German Foreign Minister Steinmeier and IOM's former DG Swing • Launched on 15 December 2017 • Mentioned in the final text of the Global Compact for Migration as a repository for data Berlin, July 12, 2016 Migration Data in Oceania Countries of Oceania Micronesia Polynesia • Kiribati • Samoa • the Marshall Islands • Tonga • Federated States of Micronesia • Tuvalu • Nauru • Palau Not included in this analysis: Melanesia • American Samoa • Cook Islands • Fiji • French Polynesia Australia and New Zealand • Guam • Papua New Guinea • New Caledonia • Solomon Islands • Australia • Niue • Northern Mariana Islands • Vanuatu • New Zealand • Tokelau • Wallis and Futuna Islands 271.6 8.7 million million Migrants originating from Oceania 2.049 million people have emigrated from a country in Oceania Migrants originating from Oceania Past and present trends • Oceania has a long history of human movement, and this history continues to influence migration patterns today • Migration is key to the region’s economy • Seasonal labour schemes facilitate migration from select Pacific countries to fill labour shortages in Australia and New Zealand • A number of resettled refugees live in Oceania • Australia maintains offshore processing arrangements for people who arrive by boat to seek asylum Recent trends • ‘Brain drain’ versus remittance gain • ‘Brain drain’ refers to the depletion of human capital in a sector or occupation in one country due to the emigration of skilled workers from those occupations to another country. • Remittances are personal monetary transfers made by migrants to individuals or communities with whom they have links.
    [Show full text]
  • The Tonga Chronicle, and Has Even Ate
    m • POLITICAL REVIEWS 195 began to reactivate local government to democracy are disrespectful to the improve its image and communications monarch and nobles, and threaten with village people. The police minister Tonga's heritage. The prodemocracy continued to speak against the prode­ supporters are equally convinced that mocracy supporters in his weekly col­ steps forward can be made peacefully umn in the government-run newspaper by a gradual education ofthe elector­ the Tonga Chronicle, and has even ate. The increasing number of non­ threatened them with violence. After government controlled newssheets, the election he wrote "The continual papers, and magazines launched in sly hints ofcorruption and dishonesty Tonga play an important role in in­ against His Majesty's Government creasing people's awareness of signifi­ without proofis going to rebound with cant issues. Several popular leaders multiple traumatic consequences on have emerged. But the cabinet together those concerned" (Tonga Chronicle, II with the nobles' representatives still Feb 1993, 3)· controls the majority in Parliament, In addition, not all members ofthe and the king retains the power to create churches are behind their leaders' call ministers of state who will support the for political reform. People have asked oligarchy. It is difficult, therefore, to that church newspapers omit political see how democratic change might comment and concentrate only on the occur in the foreseeable future, except teachings ofthe gospel and church by royal fiat which would imply an news. An advisor is to be appointed to emphatic change ofroyal heart. The counsel Free Wesleyan Church mem­ present situation, which is one of stale­ bers regarding the denomination's offi­ mate, shows that the prodemocracy cial stands on political and social issues movement still has a lot ofeducating (Tonga Chronicle, 20 May 1993, 5).
    [Show full text]