Fungal Planet Description Sheets: 1112Œ1181
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362 Persoonia – Volume 45, 2020 Lophotrichus medusoides Fungal Planet description sheets 363 Fungal Planet 1160 – 19 December 2020 Lophotrichus medusoides Calvert, McTaggart & R.G. Shivas, sp. nov. Etymology. Named for the resemblance of ascomata to Medusa of Greek Notes — Lophotrichus medusoides was isolated as an endo- mythology, a Gorgon described as a winged woman with living venomous phyte from stem tissue of Citrus garrawayi, which is an under- snakes for hair. story tree endemic to tropical rainforests in the Cape York region Classification — Microascaceae, Microascales, Sordario- of northern Queensland. Species of Lophotrichus have been mycetes. described from mammal dung and soil and are morphologically similar to Microascus, Pseudallescheria and Kernia (Sandoval- Mycelium on potato-dextrose agar (PDA) smooth, branched, Denis et al. 2016). Other taxa in the Microascales have been sub-hyaline to pale yellow, hyphae 2–4 μm diam. Ascomata reported in association with Citrus as either endophytes, e.g., perithecial, immersed or partly immersed, globose to subglo- Scedosporium, or pathogens, e.g., Ceratocystis (De Beer et bose, scattered, 250–400 μm diam, with beaks 120–200 × al. 2014). A phylogenetic analysis of the ITS region showed 30–40 μm, with peridium composed of dark brown textura Lophotrichus was monophyletic and that L. medusoides shared angularis; ascomatal appendages numerous, dark flexuous, up a most recent common ancestor with coprophilic taxa. It differs to 1.5 mm long, abundant on the beak, curved and narrowed in morphology to L. fimeti, which lacks beaks on ascomata, to at the apex. Asci evanescent, broadly clavate, 30–36 × 20–24 L. plumbescens, which has two types of ascomatal hairs, and μm, thin-walled, 8-spored. Ascospores ellipsoidal, 7.5–9.5 × to L. martinii, which has larger ascospores and shorter, wavy 5.5–6.5 μm, apices rounded, yellowish brown, with a germ pore ascomatal hairs (Guarro et al. 2012). It differs from L. indicus, at each end; wall even, 1–1.5 μm wide, smooth, extruded in a which has broad ascospores with obtuse ends and ascomata mass loosely held by ostiolar appendages. that have abundant terminal hairs (Saxena & Mukerji 1970). Culture characteristics — On PDA after 2 wk in the dark at BLASTn results of the ITS sequence of L. medusoides indi- 23 °C colonies 3.5 cm diam, flat, with sparse aerial mycelium, cated similarity to type sequences of L. fimeti (NR_154109; cream white, ascomata concentrated toward margin; with ir- Identities = 480/485 (98.97 %), no gaps), and Enterocarpus regular darkened patches after 3 wk; reverse cream white. grenotii (NR_159852; Identities = 502/530 (94.72 %), six gaps Typus. AUSTRALIA, Queensland, Iron Range, Lockhart River Rd, isolated (1 %)). The LSU sequence shared 1 056/1 096 (96 %) sequence from stem tissue of Citrus garrawayi (Rutaceae), 19 July 2019, J. Calvert identities with Cephalotrichum purpureofuscum (GenBank (holotype specimen BRIP 70690, includes the ex-type culture BRIP 70690, MF041789). A network analysis of ITS sequences showed a ITS and LSU sequences GenBank MT180536 and MT186160, MycoBank difference of three parsimony-informative characters between MB834991). L. medusoides and L. martinii (GenBank MH856648) and four between L. medusoides and L. fimeti (GenBank MF161105). Microascus longirostris CBS196.61 NR132945 99.99/100 Microascus trigonosporus CBS218.31 NR136145 Pseudallescheria ellipsoidea CBS418.73 NR130665 99.93/100 Pseudallescheria fusoidea CBS106.53 NR145213 99.99/100 L. macrosporus Kernia pachypleura CBS776.70 NR160127 L. cf. ampullus 99.72/100 Kernia geniculotricha CBS599.68 MH859190 L. fimeti strain UT-Ps2 86.73/84 L. fimeti TYPE Lophotrichus cf. ampullus UAMH11809 KM580494 L. martinii 99.82/100 Lophotrichus medusoides BRIP70960 MT180536 L. plumbescens L. medusoides 80.97/82 L. fimeti strain UT-Ps1 Lophotrichus sp. Lophotrichus martinii CBS326.50 MH856648 89.37/92 Lophotrichus plumbescens NBRC30864 LC146745 76.96/77 n = 1 Lophotrichus macrosporus CBS379.78 MH861152 n = 2 78.6/81 Lophotrichus fimeti CBS129.78 NR154109 0.05 Mid-point rooted phylogram from a maximum likelihood search using IQ- TREE v. 1.3.11.1 (Nguyen et al. 2015) with 10 000 ultra-fast bootstraps (Minh et al. 2013), 10 000 replicates of an approximate likelihood ratio test (aLRT), and a best-fit model of evolution (command -m TEST). ITS sequences aligned with MAFFT in UGENE v. 1.30.0 (Okonechnikov et al. 2012). The aLRT and Colour illustrations. Monsoon rainforest in the Iron Range, Cape York UFBootstrap values are indicated at nodes. Minimum spanning network of Pe ninsula, Far North Queensland, Australia. Ascomata showing long asco- all available Lophotrichus ITS sequences generated using POPART v. 1.7 matal hairs; textura angularis; ascospores from ruptured ascomata; 8-spored (Leigh & Bryant 2015); hashes indicate number of parsimony informative unitunicate ascus; hyphae. Scale bars = 1 mm (top left), 10 μm (all others). characters between taxa. Jed Calvert, Alistair R. McTaggart, Lília Costa Carvalhais, André Drenth & Roger G. Shivas, Queensland Alliance for Agriculture and Food Innovation (QAAFI), University of Queensland, Ecosciences Precinct, Level 2C East, GPO Box 267, Brisbane 4001, Queensland, Australia; e-mail: [email protected], [email protected], [email protected], [email protected] & [email protected] © 2020 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute.