Sequencing Abstracts Msa Annual Meeting Berkeley, California 7-11 August 2016
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M S A 2 0 1 6 SEQUENCING ABSTRACTS MSA ANNUAL MEETING BERKELEY, CALIFORNIA 7-11 AUGUST 2016 MSA Special Addresses Presidential Address Kerry O’Donnell MSA President 2015–2016 Who do you love? Karling Lecture Arturo Casadevall Johns Hopkins Bloomberg School of Public Health Thoughts on virulence, melanin and the rise of mammals Workshops Nomenclature UNITE Student Workshop on Professional Development Abstracts for Symposia, Contributed formats for downloading and using locally or in a Talks, and Poster Sessions arranged by range of applications (e.g. QIIME, Mothur, SCATA). 4. Analysis tools - UNITE provides variety of analysis last name of primary author. Presenting tools including, for example, massBLASTer for author in *bold. blasting hundreds of sequences in one batch, ITSx for detecting and extracting ITS1 and ITS2 regions of ITS 1. UNITE - Unified system for the DNA based sequences from environmental communities, or fungal species linked to the classification ATOSH for assigning your unknown sequences to *Abarenkov, Kessy (1), Kõljalg, Urmas (1,2), SHs. 5. Custom search functions and unique views to Nilsson, R. Henrik (3), Taylor, Andy F. S. (4), fungal barcode sequences - these include extended Larsson, Karl-Hnerik (5), UNITE Community (6) search filters (e.g. source, locality, habitat, traits) for 1.Natural History Museum, University of Tartu, sequences and SHs, interactive maps and graphs, and Vanemuise 46, Tartu 51014; 2.Institute of Ecology views to the largest unidentified sequence clusters and Earth Sciences, University of Tartu, Lai 40, Tartu formed by sequences from multiple independent 51005, Estonia; 3.Department of Biological and ecological studies, and for which no metadata Environmental Sciences, University of Gothenburg, currently exists. Box 461, Göteborg SE-40530,Sweden; 4.The James Poster P15 Hutton Institute, Craigiebuckler, Aberdeen, AB15 8QH, UK; 5.Natural History Museum, University of 2. Cintractiella sp., a remarkable new smut fungus Oslo, P.O. Box 1172 Blindern, Oslo 0318, Norway; parasitic on Mapania (Trib. Hypolytreae, 6.https://unite.ut.ee/acknow.php. Cyperaceae) [email protected] Abbasi, Mehrdad(1), *Eamvijarn, Amnat(1,3), UNITE (https://unite.ut.ee) is a database Flynn, Tim(2), Wood, Ken(2), Aime, M. Catherine(1) providing reference datasets and web based tools for 1.Department of Botany and Plant Pathology, Purdue the molecular identification of fungi based on rDNA University, West Lafayette, IN 47907, USA.; ITS sequences - the official barcode marker for fungi. 2.National Tropical Botanical Garden, 3530 Papalina Since 2003, when the first version of UNITE was Road, Kalaheo, HI, 96741, USA.; 3.Department of released publicly, the database has transitioned from Agriculture, Ministry of Agriculture and Cooperatives, being online DNA sequence key for ectomycorrhizal 50 Phaholyothin Rd., Ladyao, Chatuchak, Bangkok, fungi with limited geographical scope into a 10900, Thailand. [email protected] comprehensive reference database covering all fungal Cintractiella is a unique genus among smut fungi. taxa known from DNA barcode sequences globally. Sori are produced as adventitious gall-like spikelets on Current features of UNITE include: 1. UNITE Species vegetative or generative organs of members of Trib. Hypotheses (SH) - a unique way how to communicate Hypolytreae (subfamily Mapanoideae, Cyperaceae). fungal species using stable identifiers. ITS sequences Cintractiella lamii is the only known member of the submitted to UNITE and International Nucleotide genus producing its sori on vegetative organ (leaves). Sequence Databases (INSD: GenBank, ENA, DDBJ) The species was described based on a specimen are grouped into SHs by following a two-step collected from Indonesia about 80 years ago. The type clustering process, first at the subgeneric/generic level, specimen was subsequently lost shortly after then at a species level on different distances to the publication and all later descriptions of this fungus closest SH (e.g. 3%, 2.5%, etc.) All SHs are linked to have been reproduced from the original protologue. the Linnaean classification and they have a stable No other specimens of C. lamii have been reported so accession code and digital object identifier (doi) far and no other species of the genus Cintractiella on making it possible to cite and communicate them in vegetative organs of Hypolytreae, have been published studies. 2. Tools for the third-party sequence described. In September 2009 on the volcanic island of annotation of INSD sequences - used by the UNITE Kosrae, located in the eastern Caroline Islands and Community to annotate and improve the quality of within the Federated States of Micronesia (5˚20’N and fungal ITS sequences in INSD. The local copy of 163˚0’E), a specimen of Mapania pacifica was INSD dataset is updated on a regular basis. Any third- collected displaying Cintractiella-like sori in party annotation added to the INSD dataset (e.g. adventitious spikelets on the host leaves. Sori were locality, habitat, source, traits, taxon identifications, hypophyllous, occurring in groups of spikelets and interacting taxa) is made publicly available to the composed of olivaceous-brown scale like leaves, 1–1.5 research community through web services and on mm wide and up to 6 mm long. Microscopic UNITE homepage. 3. Reference datasets for sequence comparison with the protologue and drawings of the identification pipelines - UNITE provides reference type material of C. lamii show several differences in datasets with annotated sequences and SHs in various teliospore and sori characters between it and the newly collected material on Mapania. To our knowledge this *Ahrendt, Steven(1), Quandt, C. Alisha(3), Ciobanu, represents only the second known collection of any Doina(1), Clum, Alicia(1), Salamov, Asaf(1), Cheng, member of Cintractiella on vegetative organs of Jan-Fang(1), Woyke, Tanja(1), James, Timothy(3), Hypolytreae. Herein we will fully illustrate and Grigoriev, Igor V.(1,2) discuss this enigmatic genus as well as examine its 1.DOE Joint Genome Institute, Walnut Creek, CA, phylogenetic position in the smut fungi. 94598, USA; 2.University of California, Plant and Poster P2 Microbial Biology, Berkeley, CA, 94720, USA; 3.University of Michigan, Ecology and Evolutionary 4. Myxogeography of Cerrado: A first approach Biology, Ann Arbor, MI, 48109, USA. Agra, Leandro A.N.N., *Dianese, J.C. [email protected] Universidade de Brasília, Departamento de Fungi are critical research systems as they impact Fitopatologia, Brasília, 70910-900, Brazil. global carbon cycling and have remarkable potential [email protected] for sustainable biofuel production processes. Current The Cerrado, a world biodiversity hotspot, is the estimates suggest that under 10% of the estimated 1.5 largest savanna in Western Hemisphere, inserted in the million species worldwide have been described, and Brazilian territory. Myxomycetes has been studied in the majority of those described fall within the later Brazil since 1889, however in the Cerrado the first branching lineages in the Dikarya. The true extent of record, Physarum pusillum, occurred only in 1965. the phylogenetic diversity among the early diverging Major additions consisted of the 1974 survey in the fungal lineages is poorly explored at best. “Dark Southern part of the Cerrado (State of São Paulo) by Matter Fungi” (DMF) is a term used to describe Cavalcanti where 25 species were identified in uncultured and understudied fungal diversity, much of Pirassununga; and later in 1980 23 species were found which resides in these early diverging lineages. A in Botucatu by Maimoni-Rodela and Gottsberger. For polyphyletic group of these lineages can be the next 23 years sporadic records added 37 species. characterized by their aquatic lifestyles facilitated by Starting in 2003 a major effort with researchers the presence of a motile, flagellated spore. As such, connected with the UB Mycological Collection, up to they are often referred to as “zoosporic fungi” and are 2015 added 286 records to the known myxobiota of ubiquitous in the environment. Additionally, the the Cerrado. Besides that a total of 57 records were phylogenetic diversity of these lineages is estimated to revealed through collections from a Cerrado inserts in rival that of the rest of the fungal kingdom. Traditional the State of Roraima, transition Cerrado/Caatinga in high-throughput sequencing is poorly suited for DMF, Piauí, Serra do Roncador (Mato Grosso), Anápolis, since it relies on large amounts of DNA extracted from Silvânia and Pirenópolis (Goiás), completing a total many cells of the same species. Single-cell genomic 108 species in the Brazilian Cerrado sensu Batalha sequencing, however, aims to use individual cells to (2011) [47° 22' N to 28° 20' S, 73° 02' W to 40° 45' reconstruct genomes of uncultivated organisms W]. A total of 609 records representing 139 species in directly isolated from the environment. Therefore 20 localities were used to generate a set of maps environmental DMF, particularly among the zoosporic revealing a temporal evolution of the detection of the fungi, make exceptional targets for single-cell Myxomycota within the Cerrado where six genomic techniques. As much of the current single- myxogeographic gaps were filled when herbarium data cell genomic work focuses on mammalian, bacterial, and new field collections were considered. Thirty-two and archaeal systems, there is a need to develop these species were recorded for the first time in the Cerrado. protocols for