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Methods in Yeast Genetics a Cold Spring Harbor Laboratory Course Manual, 1994 Edition METHoDs 1994 "':"* edition • cfl~Js t(ai~or ~US~r~ MiChaefj~ Aamn M~tchel! - L o Methods in Yeast Genetics A Cold Spring Harbor Laboratory Course Manual, 1994 Edition By Chris Kaiser, Massachusetts Institute of Technol- of Yeast Cells; XII. Electrophoretic Karyotyping and Gene ogy; Susan Michaelis, Johns Hopkins Medical Mapping Using Pulsed-field Gel Eiectrophoresis School; Aaron Mitchell, Columbia University Col- Techniques and Protocols lege of Physicians & Surgeons 1. Yeast Transformation; 2. Lithium Acetate Yeast Trans- formation; 3. Yeast Colony Hybridization; 4. Yeast DNA The Cold Spring Harbor Yeast Genetics Course, Isolations: a. Yeast DNA Miniprep (40 ml), b. Yeast DNA founded in 1970, evolves continuously to present a Miniprep (5 ml), c. A Ten-minute DNA Prep from Yeast, d. combination of standard methods and the latest tech- Preparation of Yeast Genomic DNA (CsCi Method); 5. niques in yeast biology. This is the latest edition of Yeast Protein Extracts; 6. Yeast RNA Isolation; 7. Northern the course manual, which incorporates a variety of Analysis: Formaldehyde Agarose Gel, Blotting, and new techniques with revised experiments and the es- Hybridization to Filters; 8. Alkaline Southern Blotting Pro- sential elements from the 1987 and 1990 publica- cedure; 9. Scoring Killer Factor; 10. Hydroxylamine tions. The book remains the primary source of techni- Mutagenesis of Plasmid DNA; 11. Assay of I~-Galacto- sidase in Yeast; 12. Transformation of Bacteria (CaC! 2 cal guidance for anyone using yeast, either as a new- Method); 13. E. coli Plasmid DNA Miniprep; 14. Prepara- comer or as an established investigator. tion of Chromosome-sized Yeast DNA Molecules in Solid CONTENTS Agarose; 15. Mapping Cloned DNA Segments by Chromo- Introduction some "Fragmentation"; 16. Running Gels Using the CHEF Genetic Nomenclature Apparatus; 17. Running Gels Using the OFAGE Apparatus; Experiments 18. Yeast Vital Stains; 19. Yeast lmmunofluorescence with Looking at Yeast Cells; I. Isolation and Characterization of Antibodies; 20. Actin Staining in Fixed Cells; 21. Modified Auxotrophic, Temperature-sensitive, and UV-sensitive Lithium Acetate Yeast Transformation; 22. PCR Analysis Mutants; II. Meiotic Mapping; III. Mitotic Recombination: of Genotype Loss of Heterozygosity and Mitotic Mapping; IV. Trans- Appendices formation of Yeast; V. Complementation and Random A. Media; B. Stock Preservation; C. Yeast Genetic Map; D. Spore Analysis of Histidine Auxotrophs; VI. Gene Re- Grids; E. Electrophoretic Karyotypes of Strains for placement; VII. Isolation of ras2 Suppressors; VIII. The Southern Blot Mapping; F. Strains Formation of Zygotes: Mating and Karyogamy; IX. Clon- 1994, 202 pp., illus., appendices ISBN 0-87969-451-3 ing of Yeast Genes by Complementation; X. lacZ Gene Fu- Plastic comb binding $49 sion Expression in Yeast; XI. lmmunofluorescent Staining Reader Service No. 316 /geubl:e Tal/ g H.:¢lix F lu-.es "... the point of this story, I'II tell you right now. The answer is in DNA m Did you ever sit down and think about how the stuff that genes are made of. It is that each time a baby's born It's a baby -- not a rabbit or an ear of corn ?" pb i(i¸ : ,. i)i.:. ~ i,.? .(" • ., .f ' Written by Joe: Herskowitz. Illustrated by Judy Cuddihy. Additional text by Ira Herskowitz. 1993, Cloth (plus 12-minute audio cassette), ISBN 0.87969.431.9:$20 hiuS delightful tape-book package is The Double Talking Helix Blues is his father, geneticisi Irwin H. Herskowitz, nique way of learning about DNA interesting and fun for young people who is Professor Emeritus of Biology at and genes and how they work. (8 and up) and adults who are curious Hunter College in the City University of On tape, hear identical twin brothers about how they and their relatives became New York. Joel and Ira Herskowitz each perform the the unique individuals they are. Ira llerskowitz, Joel's identical twin Double Talking Helix Blues. Same song, brother (and 7 minutes older), is professor same genes, different styles. About the authors and illustrator: and chairman of the Department of In the book, read through the lyrics and Joel Herskowitz lives in Framingham, Biochemistry and Biophysics at the enjoy Judy Cuddihy's beautiful illustrations. Massachusetts where he practices pediatric University of California, San Francisco. Chromosomes and cells really do look like neurology. A graduate of Princeton He is a member of the National Academy this, just a bit less colorful. University (as a music major), he is on of Sciences and has been recognized for And on the back pages, be entertained the staff of New England Medical Center his scientific contributions to molecular and intrigued by a guide to heredity that Hospitals in Boston. The "Double Talking genetics by a MacArthur Foundation makes all the details clear. Helix Blues" was originally written for Fellowship and the Genetics Society of America Award. He has performed the "Double Talking Helix Blues" for students "fa[ t g H::.dis iblucs and at lectures throughout the country. Three easy ways to order.* Judy Cuddihy is an editor with Cold Call: 1-8001843-4388(Continental U.S. and Canada) Spring Harbor Laboratory Press. She O 516/349-1930 (All other locations) Cold Spring Harbor Fax: 516/349-1946 graduated from Bucknell University with Laboratory Press Write: CSHL Press, 10 Skyline Drive, Plainview, NY 11803 a degree in biology and has studied at the Parsons School of Design in New York City. Printed on recycled & recyclable paper and printed using soy-based inks. @ Reader Service No. 317 .
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