Strategic Plan – Merger of Molecular Genetics and Plant Cell Molecular Biology into the Department of Genetics and Genome Sciences at The Ohio State University

Submitted: Dec. 1, 2008 Vision

Biology has been described as the scientific discipline of the 21st century. Indeed, many of the greatest recent advances in Biology have resulted from discoveries in molecular biology and genetics utilizing model genetic organisms. Testament to this is the fact that in this decade five Nobel prizes, with profound impacts on science, technology, and clinical therapy, stemmed from discoveries made utilizing model genetic systems (2001, cell cycle; 2002, development; 2006, RNAi; 2007, ES cells; 2008, GFP).

The study of fundamental problems in Biology utilizing model genetic systems is a strength of the research endeavors at OSU. However, we propose a vision here that will markedly enhance these strengths by bringing together the two main departments that utilize model genetic organisms to answer key questions in eukaryotic biology (Appendix A, Fig. 1) - Molecular Genetics (MG) and Plant Cellular and Molecular Biology (PCMB). Individually these departments share the same vision and both are independently significant forces in the teaching, service, and research missions of the Ohio State University (OSU). To effectively increase our individual strengths, we seek to merge into a single integrated department, provisionally called Genetics and Genome Sciences (GGS). This brings together nearly all of the eukaryotic model system geneticists at OSU under a single administrative and educational unit. The merged department will be of higher visibility and have a significantly higher impact, both locally and nationally, than either parent department.

Life sciences at OSU is undergoing a major review which will result in targeted investment. In this strategic plan we set out why GGS is a prime department for such investment and our plans to utilize new resources. This strategic plan hinges upon recruitment of highly competitive new faculty as well as investment in current strong faculty through improved infrastructure and physical grouping of labs with a common intellectual focus. We will recruit new faculty members in forefront areas of genome sciences/systems biology and epigenetics who will complement our strengths in cell, molecular, and developmental molecular genetics. We will continue to partner with other colleges for faculty hiring to create opportunities for interdisciplinary research collaborations and funding. We will develop a new graduate program to attract the world’s best students. At the same time, we will design research training environments for undergraduate students, preparing them for 21st century careers in the biological sciences and programs to attract the finest postdoctoral scholars. We will partner with the Comprehensive Cancer Center (CCC) to broaden recruitment and funding of undergraduate, graduate, and postdoctoral persons. In all endeavors we will strive to recruit scientists at every level from underrepresented groups. Given investment to facilitate these goals, we expect that in 5 years we will be recognized as a leading department as evidenced by significant publications, strong extramural support, and highly ranked undergraduate, graduate, and postdoctoral training programs.

Introduction to the Current Molecular Genetics and PCMB Departments

The Molecular Genetics (MG) department, created in 1987, is a unit that employs model systems and genetic approaches to study basic biological questions. The faculty consists of 14.35 FTE positions, which has been constant throughout the past decade (Appendix A, Fig. 1). The number of faculty members (21) exceeds the FTE number, because numerous members hold joint appointments in other units, including in the Coll. Med., Coll. Human Ecol., and Coll. Biol. Sci. (Appendix A, Table 1; Appendix G, Biosketches) - a testament to MG’s role in interdisciplinary collaborations. Despite the static number of FTEs, MG has been a leader in training an increasing number of students/scholars in molecular sciences and it has been an active participant in the cross-college interdisciplinary graduate programs. Finally, MG has maintained an excellent track record of scholarly research funded by extramural grants (Appendix A, Fig. 1).

PCMB was formed in 1998 as a 10-member group with a cohesive focus on basic, modern plant molecular biology. The department hired several dynamic, high profile faculty members that have consistently published well, attracted extramural grant funding and trained students and postdoctoral associates (Appendix A, Fig. 2). However, recent retirements/resignations have left PCMB’s numbers below critical limits.

Although MG and PCMB faculty members generally use different model systems, there is considerable overlap in scholarly interests. Examples are: A. Hopper (MG), Wharton (MG), and Ding (PCMB) study RNA trafficking in yeast, Drosophila, and plants, respectively; Osmani (MG) and Meier (PCMB) use proteomics to study the nuclear envelop in fungi and plants, respectively; and numerous faculty members study signal transduction pathways in systems ranging from fungi to invertebrates to plants to vertebrates (Appendix A, Figs. 3, 4). Hence, the merger into GGS will provide opportunities to synergize our scholarly overlap and strengths. The merged department will be of higher visibility and impact than either individual department. However, to achieve the level of visibility that will bring the department to the top 20 in the country, we need to: (1) update infrastructure so that investigators can continue to conduct cutting-edge studies, (2) reorganize laboratories so that those working with similar systems or using similar technology are in close physical proximity, and (3) hire new faculty in the areas of genome sciences/systems biology and epigenetics, two areas that are of great importance to our future vitality.

Strategic Plan Goals 1. Merge the MG and PCMB departments to create a single unit (Genetics and Genome Sciences—GGS) with strengths in model systems molecular, cellular and developmental genetics and genome science 2. Build for future 21st century sciences in critical areas of molecular genetics at OSU o Recruit 6 faculty members in emerging and key areas of genetics – epigenetics and genome sciences/systems biology. o Build plant sciences - Recruit 2 Plant Targeted Investment in Excellence (TIE) faculty members o Recruit the finest faculty by offering attractive and competitive start-up packages and renovated space o Retain current strong faculty and research programs at OSU o Strengthen focuses of excellence in developmental and cell biological genetics o Update infrastructure for current and future research directions 3. Instill intellectual interactions between groups and promote interdisciplinary collaborations o Renovate Biological Sciences building to create areas for scientific interaction and discussion o Reorganize location of faculty with overlapping instrumentation needs/scholarship to neighboring labs o Locate new faculty, grouped by expertise, in renovated and attractive space o Foster interdisciplinary research programs by joint faculty appointments with other colleges o Create alliances with the Mathematics Biology Institute (MBI) by co-recruitment of systems biologists with the MBI TIE o Participate in the campus-wide Plant Molecular Biology and Biotechnology (PMBB) program, furthering the goals of the Translation Plant Sciences TIE o Increase extramural support 4. Incorporate new ideas and approaches to the learning environment o Create a new GGS graduate program - Modular curriculum requiring rigorous general courses and specializations in student interest areas - Writing workshops to result in student extramural grant applications - Additional student support via partnerships with RNA center and the CCC - NetJets Training Program o Enhanced postdoctoral fellows recruitment by participation in CCC’s T32 & NetJets Training Programs o Improve undergraduate education - Courses implementing active learning approaches and using state-of-the-art technology (e.g. MG500) - Honors general genetics course with laboratory section (MG H500) - Continue to implement enquiry based laboratory exercises - Broaden education impact by offering genetics course for professionals; increased tuition income 5. Enhance diversity o Increase involvement of undergraduates from groups underrepresented in science through participation in NSF-funded Research Experience for Undergraduate (REU) summer program o Increase graduate students/postdoctoral scholars diversity through attractive programs and fellowships

Implementation/Details of the Strategic Plan

1. Merge MG and PCMB departments. Both departments voted unanimously to merge. A subcommittee generated a document (Appendix B) describing the benefits, concerns, and strategy for this merger, which has been endorsed by the entire faculty, and we are proceeding with fulfilling the administrative requirements for the official change. With respect to administration, A. Hopper will chair the department and a vice-chair will be chosen from PCMB faculty (appropriate compensation is needed for the vice-chair). For important committees, there will be co-chairs, one from each unit, for ~3 yr to assure that both units are fully represented. Office staff are required at each of the 3 locations, but we will study whether some services can be centralized.

2. Build for future 21st century sciences in areas of molecular genetics.

Faculty hires – The search for two plant scientists via TIE positions is ongoing. We propose to recruit 6 additional faculty members, 2 at the senior level. The biological sciences develop at a rapid rate and the new discoveries seed entirely new disciplines. OSU has lagged behind in emerging key areas of importance including epigenetics and genome sciences/systems biology. GGS must hire outstanding faculty in these areas to fulfill our educational and scholarship missions and to gain prominence in the life sciences. GGS is the ideal unit to recruit such faculty, especially since many of the discoveries are being made employing model systems. Some our faculty employ genome-wide approaches; however, none are involved in technology development or conceptual growth in these new disciplines and we anticipate that addition of experts in genome sciences/systems biology to GGS will be synergistic with the current faculty (Appendix A, Figs. 3, 4). It will be necessary to “jump-start” these areas by hiring senior, well known, and highly productive faculty.

The faculty hires will build upon other strengths at OSU. For example, genome scientists focusing on RNA (“ribonomics”) will synergize with Bird, Chang, Ding, A. Hopper, Wharton in GGS and with OSU’s RNA Center; new faculty employing/creating genome-wide approaches to understanding changes in gene expression occurring during development will synergize with the strong focus of the current faculty in developmental molecular genetics (Appendix A, Fig. 3, 4). Two recruited faculty members are expected to work with plants and will be located at Rightmire/Aronoff, facilitating interactions with the ongoing recruitment for the plant TIEs. Three recruited faculty members would be located together in Bio. Sci. 9th floor renovated space. The 4th member will be located in either the 2nd or 6th floor, depending upon the match with current researchers. At present, set-up cost for junior faculty in these areas is ~$850,000; senior faculty set-ups are ~$1.3 million.

o Retain current strong faculty and research programs. The improved cutting-edge research environment made possible by faculty recruitment, combined with infrastructure improvement, and creation of attractive and functional environments (see below) will help retain excellent GGS faculty members. We established an awards committee to promote excellent faculty and we will seek favorable annual compensation adjustments.

o Infrastructure. Current faculty members do not have necessary infrastructure to assure they remain competitive within their fields. Equipment and facilities are outdated, in disrepair, or unavailable. We must purchase three confocal microscopes (one for each building occupied by our faculty), cryostat/cryofixation units at Rightmire, plant growth chambers at Aronoff and Rightmire, and genomics/robotics instrumentation at Biological Sciences. Tissue culture facilities at Rightmire need to be upgraded. These purchases, upgrades, and repairs are also required to hire top faculty members as, for example, it will be difficult to build a group in genome science without core equipment in place. The estimated cost for the necessary equipment and upgrades is $2,540,000; detailed descriptions, justifications, and costs are provided in Appendix C, Document 1). The equipment will be shared among GGS faculty as well as with faculty in other departments.

3. Promote interdisciplinary collaborations and instill intellectual interactions between groups.

o Foster interdisciplinary research programs by joint faculty appointments with other colleges. We have an exemplary record in cross-department and cross-college collaborations and faculty hires (Appendix A, Table 1). GGS will continue to partner with units in other colleges for new faculty hires. Joint recruitment builds collaborations and will provide resources to offset set-up costs for faculty hires (Appendix C, Document 2).

o Re-organize location of faculty labs with overlapping instrumentation needs and/or scholarly interests to neighboring labs; generate attractive facilities for faculty recruitment and retention. GGS has an intellectual focus but suffers from geographic spread with multiple physical locations on campus. Where possible, we will remedy this by clustering faculty with overlapping research interests or technology needs.

9th floor Bio. Sci. (Appendix D, Fig. 1): Biological Sciences which houses most of the current MG faculty is inadequate in terms of laboratory layout, air-handling, facilities, access to natural light, and places for scientific interaction. It is a poorly functional space for our current faculty occupants and has been a deterrent for faculty hiring. Fortuitously, the 9th floor is scheduled for renovation. We plan to use the renovated space to recruit new faculty, to reorganize existing faculty into neighboring labs, and to create a new learning environment for undergraduates (see 4, below, for educational activities). (a) Recruitment: Renovated 9th floor space will be used to recruit 3 genome scientists (plant genome scientists will locate to Rightmire/Aronoff). (b) Group Drosophila developmental geneticists: Seeger & Vaessin will move from Rightmire to space adjacent to Simcox currently on the 9th floor. The three labs will be integrated through sharing common equipment and specialized space. Proximity with two additional Drosophila geneticists in the adjacent Biomedical Research Tower (Wharton & Asano) will provide a stimulating local environment for students and postdoctoral scholars. Locating three new faculty members with an emphasis on genome science on the 9th floor will provide a focus in this area and also promote collaboration with developmental geneticists who already use genome-wide approaches (Appendix A, Figs. 3, 4). (c) Interaction areas: Renovation plans include an area with natural light that will serve as a scientific discussion meeting place; it will provide a much needed positive change in the working environment.

2nd and 6th floors Bio. Sci.: The 2nd floor of Biological Sciences that houses 3 molecular/cell biologists and 2 vertebrate developmental geneticists is problematic in terms of poor air handling, frequent flooding, and poorly organized and cramped laboratories. Park recently relocated from the worst space to the lab previously occupied by Oakley. Fisk and Cole are near earning promotion/tenure and we must provide for their expanded research programs to ensure their retention. Two solutions, in order of preference, are: (a) relocate both investigators to the 6th floor near Wu and Chamberlin (Wu & Fisk, and Cole & Chamberlin have related research programs). Cole & Fisk would share tissue culture facilities (Appendix D, Fig. 2); (b) create shared tissue culture and equipment rooms by dividing underutilized room #215 and modify the office suite (Appendix D, Fig. 3) to create a natural interaction area with natural light; renovated space vacated by Park could serve Chang whose research overlaps with molecular biologists, Park & J. Hopper. A 4th recruited faculty member would locate to the 6th floor or to a renovated lab on the 2nd floor, depending on the research match.

Aronoff: With Sayre’s departure, the 5th floor of Aronoff was left with two non-tenured junior PCMB faculty. Their physical distance from senior faculty on west campus was disadvantageous. The situation has been partially rectified by Meier’s relocation to Aronoff. Concurrently, a plan has been developed for space utilization and instrumentation sharing, especially important considering plans to house TIE plant hires in Aronoff.

o Increased extramural support. We will achieve increased extramural support by: (1) bringing cutting edge new technology to the department; (2) hiring outstanding research active faculty with grants or excellent potential to obtain grants; (3) instilling cooperation and collaboration between investigators through reorganization of labs with related interests and partnerships with other OSU units; (4) using the internal review process led by Leone so that all grants are scrutinized and improved before leaving the institution.

4. Provide new approaches to education. We will create undergraduate, graduate, and postdoctoral education and research training programs, funded through multiple mechanisms including entrepreneur approaches.

o GGS graduate program (Appendix E, Document 1). The merger of MG and PCMB provides the opportunity to develop a new graduate program with unique characteristics and training that will attract top students and modernize the education currently provided by each department. The 1st year of the curriculum will consist of a core series of courses–including courses in model system genetics, molecular biology, genomics, and cellular biology or biochemistry and metabolism. Components of these topics are currently being taught, but the courses will be reorganized so that redundant material will be eliminated and the courses can be adapted to the anticipated change from quarters to semesters. The core will include a new unit in mentoring and ethics. The 2nd year will consist of specialization units so, if for example, a student specializes in developmental genetics, he/she will take a different series of courses than a student specializing in cell biology. Specialty courses will be modular to enhance interdisciplinary education. Each student will enroll in a writing course to prepare for the candidacy exam and for applying for extramural and internal grants. As current courses taught by MG and PCMB service students in interdisciplinary programs, graduate students in other departments, and advanced undergraduate students, discussions with the affected groups must occur.

Eight to 10 graduate students will be recruited per year. Incoming students will be supported by research assistantships or university fellowships, as is customary in our discipline. They will conduct three 10-week rotations prior to choosing a mentor. In year 2, students will teach in GGS courses and/or in biology courses administered through the Center for Life Sciences Education. In the 3rd and subsequent years, students will be supported by their mentor’s research grants, other extramural funding, internal fellowships, or, in rare cases of grant lapses, by TA funds. Every student will apply for extramural support; we currently have a high success rate among those who have applied (Appendix E, Table 1). We will participate in training grants, e.g., the RNA/DNA group and partner with the CCC NetJets program’s graduate fellowship program (Appendix E, Document 2). With the described changes, we anticipate improved recruitment and retention, shorter times to degree, enhanced graduate placement in prestigious postdoctoral positions, and improved overall rankings.

o Postdoctoral recruitment (Appendix E, Documents 2, 3). We will improve the number and caliber of postdoctoral scholars through partnership with the CCC that sponsors a NIH T32 and the “Up on the Roof” (UoR) and NetJets Training Programs in Cancer Biology and Genetics. Several MG faculty are members of the CCC and their postdoctoral fellows are eligible for T32 slots. We committed matching funds to the UoR/NetJets programs (7 of 25 participating faculty have salaried appointments in GGS) and we have attracted postdoctoral fellows by the T32 and UoR means (Appendix E, Document 3).

o Improved undergraduate training. The MG undergraduate major is one of the largest majors in Life Science and it will remain as is after the departmental merger. Though smaller, the PCMB major offers important service courses for a Land Grant university and will be continued, although the possibility of creating a cross-campus undergraduate Plant Science major will be explored. Several new initiatives to improve undergraduate education will be implemented; the predicted increases in enrollments will provide new funding.

Undergraduate lab/research: A renovated 9th floor Bio. Sci. lab will be devoted to undergraduate teaching/research - the NSF-funded REU program for underrepresented students, the International NetJets program, MG601/MG602, new problem based laboratory experiences, and the Honors MG500 laboratory.

Genetics for professionals: Understanding genetics and genomics is increasingly important and has created a need for general genetics courses for professionals—including MDs, lawyers, scientific writers, and agricultural managers, etc. To maximize the benefit and accessibility for these professionals, we will offer courses at convenient times (e.g., evenings) and provide modified presentations to meet their background/interests. Judging from past requests, we anticipate high enrollment (Appendix C, Document 2). Increases in GGS faculty numbers will provide the staff required to offer this course.

Redesigned MG500: The importance of genetics is also apparent in the undergraduate population. Our general genetics course (MG500) has an ever-increasing enrollment (Appendix A, Fig. 1). The large number of students and their very different backgrounds offer a challenge for instructors. In response to this, Vaessin, Seeger, & Booton designed a new format (Appendix E, Document 4) incorporating multiple media resources, teaching assistant support, peer interaction, traditional lectures, and problem-solving opportunities in a central active learning center. It will be conducted alongside a traditional MG500 section to quantitatively assess its success. Redesigned MG500 will have cost savings while enhancing education (Appendix E, Document 4).

New Honors MG500 lab: The honors version of MG500 has not been offered for the past 2 years due to staffing issues. Redesign of MG500 provides the opportunity for honors students to enroll in this active learning course by addition of special laboratory/recitation exercises, allowing the course to meet the enhanced requirements of an honors experience while generating new income (Appendix C, Document 2).

PCMB101/102: The undergraduate, laboratory courses PCMB 101/102 have a significant clientele. We will modernize their content. These courses have potential revenue for lab fees (~400 students/year, presently).

Research oriented laboratory courses: GGS faculty members have promoted enquiry-based learning by incorporating original research in undergraduate lab courses, facilitated by their successful competition for funds to acquire state-of-the-art equipment. Fisk developed a MG602 module for students to learn molecular & cell biological approaches by determining the localization/function of proteins identified by proteomics. In MG601, Simcox employs microarray analysis for original research questions. Lamb purchased plant tissue culture equipment and growth chambers for PCMB 300 & 648, permitting student designed multi-week growth experiments. Teaching evaluations document that students appreciate conducting original research. The 9th floor teaching lab will not only accommodate the existing courses, but it will also allow new efforts by others to increase use of original research in undergraduate education.

5. Diversity initiatives. Simcox is PI of a successful NSF REU grant devoted to original research opportunities for underrepresented undergraduates. GGS has several women and some minorities among its ranks, but we seek to generate more diversity in the next 5 years. Efforts will include faculty participation in recruitment trips to minority serving institutions, attracting graduate students from the REU program, and attractive fellowships.

Metrics

Graduate Programs - Academic Affairs provided AAU shared data for Genetics/Plant Sciences PhD programs obtained from 3 NRC fields: Genetics & Genomics, Cell & Developmental Biology, and Plant Sciences (Appendix F, Table 1). OSU graduate students have equivalent GRE scores.

Departments - A document generated by CBS (Appendix F, Fig. 1) shows that MG and PCMB have considerable strengths among CBS units. Metrics comparing GGS with other like departments are difficult to obtain due to the diverse means by which these disciplines are organized (Appendix F, Table 2). We generated a data set for institutions with model systems emphasis and/or departmental organization similar to OSU (Appendix F, Table 3). OSU has fewer faculty members than the comparison units; other metrics are similar. U. Wisconsin Genetics, also a land grant university, is consistently ranked in the top 10 by the Chronicle of Higher Education (http://chronicle.com/stats/productivity/page.php?year=2007&institution) and US News World Reports. U. Wisconsin kindly provided detailed information regarding publications, extramural funding, national recognition awards, etc. - criteria used by the Chronicle to rank units. Comparison of OSU to U. Wisconsin (Appendix F, Tables 3, 4) is somewhat complicated because the Wisconsin genetics departmental faculty members are in both the College of Medicine and Biological Sciences. Nevertheless, OSU’s student population appears equivalent, but Wisconsin faculty members are better funded and their publications are more often cited. We aim to improve in these criteria and to approach our U. Wisconsin peers in the next 5 years. Investment in our merged department will make it possible to achieve this goal.

Appendix A

Description of MG and PCMB Departments and Faculty Appendix A, Table 1 Joint Appointments for Molecular Genetics Faculty Members

Faculty member Molecular Other Academic Appointment Genetics % Appointment

Asano, Maki, Assistant Prof. 20% Molec. Cellular Biochemistry, Coll. Med. Bird, Amanda, Assistant Prof. 20% Human Nutrition, Coll. Human Ecology Bisaro, David, Professor 50% Plant Biotechnology Center Chamberlin, Helen, Assoc. Prof. 100% NA Chang, Tien-Hsien, Assoc. Prof. 100%, on leave NA Cole, Susan, Assistant Prof. 100% NA Doseff, Andrea, Assoc. Prof. 40% Internal Medicine, Coll. Med Fisk, Harold 80% Molec. Virology Immunology Medical Genetics, Coll. Med. Herman, Paul, Assoc. Prof. 100% NA Hopper, Anita, Professor 100% NA Hopper, James, Professor 40% Biochemistry, Coll. Biol. Science Leone, Gustavo 20% Molec. Virology Immunology Medical Genetics, Coll. Med. Osmani, Stephen, Eminent Prof. 100% NA Park, Hay-Oak, Assoc. Prof. 100% NA Seeger, Mark, Assoc. Prof. 50% Center Molecular Neurobiology, Coll. Med. Simcox, Amanda, Professor 100% NA Vaessin, Harald. Professor 50% Center Molecular Neurobiology, Coll. Med Verma, DeshPal, Professor 50% Plant Biotechnology Center Weinstein, Michael 80% Molec. Virology Immunology Medical Genetics, Coll. Med. Wharton, Robin, Professor (2009) 60% Molec. Virology Immunology Medical Genetics, Coll. Med. Wu, Jian-Qiu, Assistant Prof. 75% Molec. Cellular Biochemistry, Coll. Med.

Appendix A, Fig. 4 Example Interaction Between New Faculty Hires and GGS Current Faculty

mitosis and nuclear architecture genomics new hires

flies

development

Appendix B

GGS Merger Plan The Plan for a Merger of the Departments of Molecular Genetics and Plant Cellular and Molecular Biology: And Its Relationship to the Translational Plant Science TIE

This is a reply, from the faculty of the Departments of Molecular Genetics (MG) and Plant Cellular and Molecular Biology (PCMB), to the Aug. 27, 2008 OAA/OR Assessment of the Translational Plant Science (TPS) TIE. This is not meant to replace the formal response of the TIE program itself, which will come from the TIE administration.

The faculty of both MG and PCMB participate substantially in the TPS TIE. Presently, MG and PCMB are proceeding toward merger into a single Department. Details of all aspects of the merger are actively being developed. Hence, this document addresses: 1) The historical perspective of this merger, including the relationship between these Departments and their roles in the TPS TIE; 2) An overview of merger issues and status; and 3) A preliminary statement of the future vision of the new unit – whose synergy goes beyond a sum of the parts and which will be fully addressed in the joint Strategic Plan to be submitted late in Autumn Quarter.

Historical Perspective

The Department of Molecular Genetics, in existence for 20+ years, has grown from a minimal faculty size to 18 members, represented by a total of 14 FTEs. The members are diverse in their research foci and very productive. Research of the salaried faculty focuses on many model organisms, from yeast to animals and plants. The Department of PCMB was formed in 1998 when the plant ecologists and evolutionists of the Department of Plant Biology joined the animal ecologists to form a new unit, the current Department of EEOB. That original PCMB unit consisted of nine plant molecular and developmental biologists, the number of which grew to 12 before recently declining to seven due to unreplaced departures and retirements. Over the past several years the faculty of both MG and PCMB have been highly productive in quality and quantity of research publications and per-faculty research grant spending rates.

Both Departments have strong interactions with faculty in other Departments, within and outside CBS. In MG, faculty with primary appointments in a number of other units participate in graduate education, teaching, and service. Notably, strong affiliations with the OSU Medical Center have been established. PCMB also hosts courtesy appointees from CFAES and CBS, and one faculty member has a minority appointment in a Medical department.

PCMB and MG faculty participate in the Plant Molecular Biology and Biotechnology (PMBB) Program, which draws membership mainly from CBS and CFAES. It is not an overstatement to say the PCMB and MG members are part of active core of PMBB. The TPS TIE proposal was developed by the PMBB group, led by its Director, Robert Tabita, with input from PCMB, MG and CFAES faculty. The TPS TIE is administered through PMBB, as described in the past year’s TIE report. A proposal for a novel interdisciplinary/intercollegiate/international graduate program in Translational Plant Science (TPS) is presently under review, and BOR funds for support of graduate study in the present PMBB Specialization (associated with PCMB, MG and plant CFAES graduate programs) have already been awarded.

The Translation Plant Science TIE

The TPS proposal called for five junior faculty hires, two in CFAES and three in CBS, with associated cash awards. Much of the program is presently being implemented, as recognized in the Aug. 27 TIE Review: However, the associated OAA/OR Assessment unfortunately includes several major factual errors, which essentially negate its conclusions – the italicized lines in the next paragraph address these errors.

Here is a summary of the TPS TIE to date. One CBS and one CFAES hire are presently at OSU (neither of these are even close the stage for tenure evaluation). An additional CFAES position is presently in the searching process, and two CBS positions to be housed in PCMB were not authorized last year (i.e., there were no new hires this year because the searches were delayed by the Dean for financial reasons), but have been scheduled for searching this academic year. Also, a senior search in PCMB, not a part of the TPS TIE or the PMBB program, was conducted in 2006-2007. In this search, one applicant declined an offer, while two additional interviewees remained interested. As the Department was seeking additional applicants in July 2007, the search was suspended (i.e., this is a suspended, not failed, search). We are very optimistic that a high-level candidate could have been attracted for this position. One major point of this document is to demonstrate that a faculty merger, suggested by the Assessment as a way of strengthening this TIE, is already in progress.

The Merger of the Departments of MG and PCMB

Rationale

The Departments of PCMB and MG share substantial common interests in genetics, developmental and cellular biology, and functional genomics as well as systems biology. The present size and lack of prospects for expansion make it very difficult for PCMB to compete in the present OSU landscape, and this is true to a lesser extent for MG. However, the combined Department will represent a group that will be very strong in all aspects – research, teaching, etc. With appropriate administrative support, and building on the extensive synergy of the two groups, it can significantly expand its visibility outside the University. Hence, the two faculties are proceeding with the merger process, with the goal to administratively fuse and complete the other aspects of the process as quickly as possible.

Present Status of the Merger

Discussions among individuals of the Departments led to the appointment of a Joint Merger Committee in Summer, 2008. This Committee (formed by faculty from PCMB and MG and) in consultation with the Chairs, produced a document which addressed the steps and issues of a merger. The final version was communicated to both Chairs on August 22, 2008. The document was approved at a PCMB faculty meeting of August 26 and the MG faculty meeting of September 12 - and the merger process has formally initiated.

Moving Forward

Many issues need to be addressed for the merger to proceed smoothly. Joint committees have been appointed to address items such as graduate program structure, curriculum, undergraduate majors, etc. Overviews for some key components follow.

Graduate Program

The students of the present MG and PCMB graduate programs publish at a high level during their graduate careers, move to prestigious post doctoral positions and become productive independent scientists. The merged Department will adhere to and advance these characteristics. This department will operate a single graduate program. A major strength of the program will be the common theme of using model systems for life sciences research. Moreover, the combined faculty provides training in a comprehensive range of these systems. The curriculum will be developed to streamline formal course work, while maintaining rigor, and allowing students to develop depth in topics related to their own specific interests. In addition to the departmental program, faculty will also be active members of the larger 'integrated' programs, such as MCDB. These programs complement the more focused departmental program, as they tend to attract students who enter graduate school initially looking for a broader range of research options.

The interface of this Department’s program with the interdisciplinary PMBB Program represents an opportunity for collaboration to maximize the effectiveness of basic plant biology training at the University. The developing tri-partite cooperative plant program between OSU, the University of Sao Paulo, Brazil and Rutgers University is a very promising collaborative program that is just getting started between OSU plant programs and these other two universities. This will, of course, be continued in the new Department and should represent one of its strengths. The existing plant-related programs in CFAES are very distinct from ours, and almost certainly should be maintained separately from PCMB/MG.

Curriculum

Both Departments presently offer full programs of undergraduate and graduate courses. Likely, some specialized offerings can be eliminated, and wherever possible we will create combined syllabi for existing or new courses that will build on the new interface. Ultimately, it is anticipated that a unified curriculum will result that will serve the full diversity of graduate and undergraduate education that is presently available. A curriculum committee has been appointed to examine these issues.

Undergraduate Majors

The high-quality MG undergraduate major represents one of the larger groups of students in the College. Clearly, it should not be disturbed. The PCMB major embodies an essential academic program for a Land Grant university in an agricultural state. This major is smaller, but offers several important service courses. Hence, the two majors will be continued, although the possibility of creating a cross-campus undergraduate Plant Science major will be explored.

Long-Term Vision

The synergy provided by the merging of PCMB and MG will result in a unit with the promise to become top ranking nationally and internationally in research and education in the areas of cellular and developmental genetics, genomic sciences and systems biology of model eukaryotic organisms. These are areas in which OSU is lagging considerably behind other universities, despite constituting the bases for most of the applied sciences, including medicine and agriculture. A significant investment in new hires and infrastructure will be required to project the new department to the high-visibility level that it deserves, based on the achievements of the individual faculties involved.

Appendix C

Infrastructure and Cost Analysis Appendix C, Document 1 - Infrastructure Request , Three Confocal microscopes: Confocal microscopy is a “must have” technology for cellular and molecular biologists. An outdated instrument exists in Rightmire; there are none in Aronoff or Bio. Sci. Due to the nature of the experiments and biological systems and to the frequent need for imaging (especially with relocation of all Drosophila developmental labs from west campus to Bio. Sci. and anticipated plant hires at Aronoff), multiple shared instruments are required, each costing ~$300,000, totaling $900,000.

Cryostat/Cryofixation units: To fully understand the macromolecular interactions within a cell, the foundation for development, physiology, and infection, the precise subcellular localization of molecules must be determined. Proteins and RNAs are subject to breakdown and re-location when tissues are processed for microcopy by conventional chemical fixation. Cryofixation minimizes the problems by physically immobilizing proteins, RNAs, etc. in situ. A cryostat ($50,000) will freeze samples at ~–300C, allowing cryosections to be obtained for high-resolution light microscopic studies. There is also a need for electron microscopic level subcellular localization and trafficking of molecules. For EM procedures a propane jet freezer ($30,000) that ultrarapidly freeze samples to – 1800C is required. Ding is an expert cryomicroscopist and will be in charge of the facilities. They will serve the needs of Rightmire faculty and faculty in the new department and the PMBB.

Genomics instruments (a) “Deep” DNA sequencing facility. The Solexa type of facility has taken over current technology and will replace microarray facilities. Top ranked research institutions have multiple units, e.g., 5 in Genome Sciences at U. Washington. The Coll. of Medicine just purchased OSU’s first, but it will be over-subscribed and it will be difficult to build a group in genome sciences in CBS without such an investment. Cost: ~$800,000. (b) Robotics: High throughput experiments require robotics. Most modern genetics departments have this technology; however, there are no such instruments in MG or PCMB. Cost: ~$300,000. The genomics facilities will be located in the renovated 9th floor of Bio. Sci. as it will be required by new hires; the facilities will be shared by other departmental members as well as by members of other CBS departments.

Greenhouse upgrades and growth chambers - Plant researchers need plant growth space. Four growth chambers (each ~$65,000; $260,000) are required to accommodate the anticipated 5-6 plant groups at Aronoff. The greenhouse facility/plant growth rooms at Rightmire are in disrepair; it will cost about $100,000 for their repair.

Tissue Culture facilities at Rightmire - With controlled lighting, humidity and temperature conditions, the tissue culture room is vital to the research programs of all faculty housed in Rightmire Hall. The cell cultures maintained here are used for studying gene expression, subcellular trafficking of macromolecules and metabolites, and pathogen infection in single cells. All transgenic plant work also starts in this room, by culturing the initially transformed cells and tissues into plantlets before growing them in greenhouses and growth chambers. The control panel, air-condition system and lighting system designed and constructed over 20 years ago started to show malfunction in the last 3 years. Several repairs have been performed. However, they are costly and do not solve the fundamental infrastructure problems. The requested funds ($100,000) will allow installation of state-of-the art systems to ensure normal functioning of the culture room, eliminating costly downtime of research activities and minimizing maintenance expenses.

Appendix C, Document 2 – Finances

Requests:

1. Vice‐chair administrative addition $10,000/yr ‐ continuing 2. Positions 2 Plant TIE ? 6 GGS faculty hires, 2@ senior level $4,800,000 1‐time set‐up assuming 80% appointments 3. Infrastructure $2,540,000 1‐time Bio. Sci., Aronoff, Rightmire 4. Renovations moving gas on 6th floor ? replacement of shared equip. $15,000

Potential earnings:

1. Single Chair ~$40,000/yr ‐ continuing 2. new MGH500 ~$25,000/yr ‐ continuing (2 sections/25 students each) 3. new format MG500 ~$133,000/yr – see Appendix E, document 4 4. new Genetics for professionals ~$45,000/yr ‐ continuing 50 students/yr 5. new Grant income indirect costs ?

Appendix D

Renovation/Relocation Floor Plans Appendix D, Fig. 1- Ninth floor Bio. Sci.: developmental genetics, genomics, teaching lab, and MG/PCMB office

genomics core

Simcox darkroom

Seeger Seeger

Vaessi n Vaessin

fly culture 17°

Simcox lab instructor/ teaching support

tissue culture mail room/ imaging/ office support fly room confocal Appendix D, Fig. 2 – 6th floor Bio. Sci.

Cole lab Common Fisk/Cole joint and office equipment tissue culture

Wu lab and Fisk lab Chamberlain office and office lab and office Appendix D, Fig. 3 – Possible 2nd floor Bio. Sci. improvements

Fisk/Cole Build wall tissue Common Common Fisk lab culture Hopper lab equipment equipment Hopper Office

Fisk office

Park office

Knock out walls, open lounge with windows Cole office

Weinstein lab& office Storage and Cole lab Park lab office for MG manager Conference room

Appendix E

Education Appendix E, Document 1 - New MG-PCMB merged graduate program

Currently, the following are the core curricula for the two existing programs:

PCMB PCMB622 (4) Plant Molecular Biology PCMB623 (4) Plant Genetics & Genomics PCMB735 (3) or PCMB736 (3) Plant Biochemistry PCMB630 (3) or PCMB631 (3) Plant Physiology PCMB643 (5) or PCMB648 (4) Plant Anatomy/Cell Biology

MG MG700 (3) Systems of Genetic Analysis MG701 (3) Molecular Genetics: DNA Transactions MG705 (3) Advances in Cell Biology MG 880.06 (3) Regulation of Gene Expression: Transcriptional Control MG 715 (3) Developmental Genetics MG 880.07 (3) Regulation of Gene Expression: Post-transcriptional Control

For the new program, we discussed having the following core courses (names could be changed). We anticipated that once we move into a semester system, the number of credits of the 4 cr courses could be decreased to three, making the transition smoother.

Model Systems Biology Graduate Program Core Curriculum (17 credits) 1. MG700 (4) Systems of Genetic Analysis (MG700 & genetic aspects of PCMB623) This class increases by 1 credit hour to accommodate plant module in addition to the yeast, invertebrate and vertebrate modules.

2. MG701 (4) DNA and RNA Transactions (MG701 & PCMB622 - include transcription) The genomics half of MG701 will be excised (see below) and transcription and post- transcriptional regulation will be added (from PCMB622 and Biochem702). Number of credit hours will be increased to 4. This will unify related topics into an advanced molecular biology/central dogma class.

3. MGXXX (4) Genomics (PCMB623 & genomics content of MG701)

4. MG705/PCMB648 (4) Advances in Cell Biology OR Biochem614 (4) Metabolism Students will choose one of these two courses based on their interests and previous academic background. The graduate level core course in Cell Biology should cover a number of topics at an advanced level (beyond MG607).

5. XXXX (1) Mentoring/Ethics Course This course is currently offered to MCDB and OSBP students. We need to incorporate this course to be eligible for training grants, etc.

Specializations (10 credits) (* Indicate mandatory course for respective specialization) Students can take any other courses from the department or the list of electives to complete the 10 credits of the specialization

DEVELOPMENTAL GENETICS 1. MG715 (3) Developmental Genetics* 2. MG880.01 (4) Advanced Topics in Developmental Biology* Two-week long modules in five specific topic areas (possibly one week of lectures and one week of student paper presentations). Module topics would depend upon the faculty member teaching a module and may change from year to year. Examples could potentially include: Circadian Biology (Somers), Vertebrate Segmentation Clock (Cole), Development of the Vulva (Chamberlin), Specification of Neural Crest Cell Fates (Henion), Smads (Weintstein), Evo/Devo (Seeger and Chamberlin), Flowering, etc. 3. XXX (1) Scientific writing* (Course to be taken by all students in their last quarter/semester of the second year in preparation for the ACE) 4. Neruo790(3) Developmental Neuroscience

GENOMICS AND SYSTEMS BIOLOGY 1. XXX (3) Topics in Genomics* 2. XXX (3) Topics in Systems Biology* 3. XXX (1) Scientific writing* (Course to be taken by all students in their last quarter/semester of the second year in preparation for the ACE)

CELL BIOLOGY 1. MG880.02 (4) Advanced Topics in Cell Biology (see Developmental Biology section for the concept) 2. XXX (1) Scientific writing* (Course to be taken by all students in their last quarter/semester of the second year in preparation for the ACE)

PLANT MOLECULAR BIOLOGY 1. PCMB630/631 (4) Plant Physiology* 2. PCMB735/736 (4) Plant Biochemistry* 3. XXX (1) Scientific writing* (Course to be taken by all students in their last quarter/semester of the second year in preparation for the ACE)

General electives STAT528 (4) STAT529 (4) MG880.06 (3) Regulation of Gene Expression: Transcriptional Control MG880.07 (3) Regulation of Gene Expression: Post-transcriptional Control PCMB643 (4) Plant Anatomy

Implementation Timeline Autumn 2008: Planning the new merged graduate program. Winter and Spring 2009: 1) Merging of student research presentations from both the MG and PCMB Graduate Program. 2) Initiate the approval process for the new graduate program. 3) Coordinate graduate student recruitment for the 09/10 MG and PCMB graduate student classes. These will be last incoming classes of the separate graduate programs. Autumn 2009: Website for the new graduate program goes live. Winter 2010: Recruitment for the first class of the new graduate program who will start Autumn 2010. Autumn 2010: The new core curriculum is initiated.

Sample Quarter System Schedule First year Autumn: Genetic Model Systems (4), Adv Molecular Biology (4), Bioethics-Mentoring (1) Winter: Cell Biology OR Biochemistry (4), Genomics (4) Spring: Electives (6 to 8)

Second Year Autumn: Advanced Topics Seminar Class (4) Winter: Writing Class (1) Spring: Remaining electives

Sample Semester System Schedule First year Fall: Genetic Model Systems (3), Adv Molecular Biology (3), Bioethics-Mentoring (1) Spring: Cell Biology OR Biochemistry (3), Genomics (3), Elective (2-3)

Second Year Fall: Advanced Topics Seminar Class (3) Spring: Writing Class (1), Electives

Appendix E, Document 2 - Funding for training – NetJets Program NetJets Training Program in Cancer Research (Annual Symposium – $30K)

Undergraduate Graduate Pre-doc Postdoctoral Fellow. Program Program Program (summer) (2 yrs) (2 yrs)

• 10 International Exchange Awards • 20 Awards • 12 Awards (National and Intern.) - $5K awards + $2K travel - $25K stipends $50K + 6% Benefits + $2K travel - Hyderabad (India) - Tuition & Fee waivers • 50 Participating Faculty - Asia (China/Taiwan/Singapore) • Faculty $4K Contribution • 15 OSU Awards • Department $4K Match - $4.5K awards (+ $500/lab)

Cost = $145K $500K $660K

Total Funds = $1.4M

Total Cost = 1.305M (3 programs) 0.040M (Symposia) 0.050M (postdoc recruitment) $1.395M

Guttridge, Kirschner, Simcox, Byrd and Leone 1) One culture in science? Most scientists will say that there is a universal code that dictates how scientific research should be performed and will describe the system using words and phrases such as rigor, the testing of original ideas (creativity), reproducibility, and open dissemination of results. The ultimate goal is to uncover the true mechanism for the process under study. But, we know that culture shapes how science is carried out.

2) Global mobility. OSU students (and indeed most of their US counterparts) typically train and remain in the US for their entire careers. In contrast, many scientists in India leave home to train and work in the US. This westward migration, the so-called ‘brain drain’, was prevalent for a number of years. But the trend is changing and today it is more aptly described as ‘brain circulation’ or a ‘global diffusion’ of science. Scientists return to their home counties in greater numbers and there is a new trend for prominent US scientists to move to institutions in the rest of the world including new centers of excellence in Asia. This mobility of scientists is part of the globalization seen in many spheres, perhaps most notably exemplified by the information technology (IT) field in which India is already a world leader. Within a few decades India and other Asian countries will also develop as major players in science. Will OSU students be ready to capitalize on this development? Can they picture themselves living and working in India where new and promising job opportunities might arise? Are they prepared to exploit the global mobility their training in life sciences affords them? Clearly, for Indian students the idea of global mobility is not new and many Indian scientists live and work in the US and all around the world. We expect the dialogue between the US and Indian students will initiate the process whereby eventually we will see more reciprocity in scientist training between the two countries.

3) Enhancing the global presence of OSU. Finally, we also anticipate our proposal to fulfill an important mission of OSU’s international outreach activities in promoting open resources, technology and teaching practices, and inspiring knowledge and learning at a global level.

“Up on the Roof” Postdoctoral Program

in Cancer Biology and Genetics The Ohio State University

High caliber individuals with a strong record are invited to apply for six postdoctoral positions to study various molecular and genetic aspects of cancer. The Program is highly interactive and multidisciplinary, and fields of research range from the identification of novel oncogenes and tumor suppressor genes, to the control of proliferation, cell cycle and apoptosis, epigenetics, transcription regulation, signal transduction pathways, immunology, mouse tumor models, bioinformatics, and computational biology of cancer models. The program has had a history of placing our postdoctoral fellows in tenured assistant professor positions or equivalent positions of leadership. For a more detailed description of the research interests of participating faculty members please see our web page at http://www.cancergenetics.med.ohio-state.edu.

Maki Asano, MD, PhD – Genetic analysis of DNA replication control Michael Caligiuri, MD - Molecular biology of acute leukemia and NK cell biology William Carson, MD - Immunotherapy of cancer. Albert de la Chapelle, MD, PhD - Genes predisposing to cancer and expression profiling in cancers Carlo Croce, MD, - Identification and characterization of genes involved in pathogenesis of cancer Harold Fisk. PhD – Centrosome assembly; from molecular mechanisms to its role in genomic stability Joanna Groden, PhD – Mouse models of gastrointestinal cancer; biology & biochemistry of DNA repair Denis Guttridge, PhD – NF-kappa B signaling in cell growth and differentiation Tsonwin Hai, PhD - Stress responses in stroma-cancer interactions, inflammation, and metastasis Anita Hopper, PhD – Intracellular trafficking of RNA & proteins; Nucleus organization, RNA processing Tim Huang, PhD – Epigenetic profiling of gene silencing in cancer Lawrence Kirschner, MD, PhD – Mouse models of endocrine tumorigenesis; PKA signaling Gustavo Leone, PhD - Cell cycle and microenvironment in development & cancer Stephen Osmani, PhD – Mitotic regulation of nuclear structure Michael Ostrowski, PhD – Genetic analysis of Ets family members in development & cancer Deborah Parris, PhD - HSV: mechanisms of RNAi silencing suppression and DNA replication Mark Parthun, PhD – Histone post-translational modifications and the assembly of chromatin structure Jeffrey Parvin, MD, PhD – Biochemistry and molecular biology of breast cancer Kamal Pohar, MD – Genetics and animal modeling of bladder cancer Matt Ringel, MD – Signaling in thyroid cancer invasion and metastasis Saïd Sif, PhD – Aberrant chromatin remodeling and histone arginine methylation in leukemia/lymphoma Amanda Toland, PhD - Genetics of cancer susceptibility Robin Wharton, PhD - Translational control of nuclear proliferation in Drosophila Jian-Qiu Wu, PhD - Cellular asymmetry and cell division in fission yeast Sung Ok Yoon, PhD - JNK signaling in neurodegenerative diseases

The OSU Comprehensive Cancer Center is a state-of -the-art basic and clinical research institute with impressive Genomics, Proteomics and Transgenic Core Facilities. Interested applicants should send curriculum vitae, statement of research interests, and the names and addresses of three references to Liz Stranges, Human Cancer Genetics Program, 460 West 12th Ave, Room 809B, Columbus, OH 43210; or by e-mail to [email protected]. Application deadline is January 30, 2009.

The Ohio State University is an equal opportunity/affirmative action employer. Qualified women, minorities, Vietnam era veterans, and individuals with disabilities are encouraged to apply. Appendix E, Document 4 - Course Redesign: Molecular Genetics 500, General Genetics

Teaching Goals: This course should provide students with a robust survey of the principles of genetics, including molecular genetics, transmission genetics, developmental genetics, non-chromosomal genetics, and the genetics and evolution of populations. In addition, recent advances in genetics and the implications/effects of these advances on science, business, politics, and society in general are addressed throughout the course.

Student Goals: At the end of this course the student should have a broad, robust knowledge of classical and molecular genetic concepts, and of basic fundamental current molecular genetic techniques. Further, at the end of this course the student should have the background, knowledge, and ability to carefully understand, interpret, and critically analyze future breakthroughs in molecular genetics and biology.

Background: • Class sizes vary presently between 150 and 280 students (generally with waiting lists at the start of quarter). • MG500 is required course for most life science majors. • Approximately 900 to 1000 students enroll into MG500 each year. MG500 is offered every quarter (including Summer) and has two course offerings in Winter and Spring quarters. • MG500 teaches key skills/understanding that every life science major should have mastered at a reasonably advanced level. • Students enroll into MG 500 generally as junior or seniors. For many students MG 500 is the “last (big) hurtle” or ‘gatekeeper” before graduation. It is not uncommon to encounter students who have to take this course multiple times

Challenges with present format: • Material that is taught at other institutions in one semester has to be compressed into 10-week quarter. • To achieve critical competencies in genetics extensive problem solving is essential. The present format does not support this need very well. • Educational preparedness to take this course varies widely: Approximately one third of enrolled students appear to be ill-prepared. Possible causes could include time since last relevant biology courses, including transfer credits and/or AP credits. In contrast, between 30 to 35 percent of enrolled students are pre-med, pre-vet, etc. and generally well prepared. • While interests of majority of students appear to be directed towards health sciences, a portion of students comes from majors with a different emphasis. At present it is difficult to address the specific interests of these students. • A subset of students fails this course multiple times. This directly affects their time to graduation or capacity to graduate at all. • Monitoring of academic at-risk students and timely intervention is difficult in present format, and relies heavily on students actively seeking help. Specific Objective: To develop a redesigned MG500 “concepts of genetics” course that significantly increases the proportion of students who obtain proficiency in the teaching and course objectives of the current MG500. We seek to achieve this goal with the development of a course that incorporates multiple media resources, teaching assistant support, peer interaction, traditional lectures, and problem-solving opportunities in a central molecular genetics active learning center. Our initial course structure would be a set of four “concepts of genetics modules” that will incorporate the subject matter currently covered in this course. Before moving on to the next module, students would be required to demonstrate proficiency in each module. Each module would cover two weeks of instructional course time. The first week of the quarter would be used to instruct students in the overall features of the course and as a chance for students to become comfortable with the structure and resource availability of the course. The final week of the course would be used to allow the students, after having demonstrating proficiency in the course material, to explore a variety of subjects of their choice in greater depth. Demonstrated proficiency of this tenth week material would also be required. The proficiency examinations that student will be required to complete throughout the course will account for 70% of the overall grade of the course. We then propose a comprehensive final for the remainder of the course evaluation: 30%. We feel it is important to establish a measure at the end of the course to evaluate overall student comprehension of the material, as well as a method to compare to overall comprehension of the course material in the traditionally taught MG500 We feel this course redesign model will increase the number of students who achieve the desired learning outcomes for this course, while simultaneously directing and maximizing our resources towards our educational goals.

Course Re-design Process Elements:

Assess present course system (Spring quarter) Intro test, Exit test (Final exam?)

Define: Define desired overall learning outcomes/goal Module areas Sub-module areas Formulation of desired key competency/learning outcomes for each module/sub- module.

Identify: Available adaptable module elements (commercial or otherwise; web-based) New to develop modules Technology requirements/availability

Develop course redesign Structure Curriculum Implementation

Draft MG500 Course Modules

Introductory Module: Week 1

Course introduction, learning center introduction, pre-testing through online quizzes etc (which will not count towards final grade) but will allow instructors to establish a baseline of student knowledge of genetics prior to taking this course. Therefore this week will serve a dual purpose of student introduction to the course and a source of data for the instructors to determine the knowledge level of incoming students in the course.

Weeks 2 to 9: Modules 1 to 4:

Draft Individual Module structure:

1. Monday lecture, module week 1 (1 hour, 18min). A traditional one-hour, 18 minute lecture at the beginning of the first week of module that introduces and outlines the material that the student will be expected to learn that particular week. Background material that generally makes up a significant portion of current traditional MG500 lectures will not be directly taught during lecture hours and students will be referred to guided readings and online resources for those who may not be proficient in this material.

2. Student Learning Center Study. Following the first Monday lecture, during the early part of the week, students are expected to complete assigned reading and online learning activites. In addition they will work with teaching assistants and their peers in a computer-based learning center where online problem sets, quizzes, and evaluations of comprehension and understanding of each module can be carried out. Students may take practice quizzes etc. during this time to determine their level of understanding of the current module’s material. Results of these quiz results will be forward to MG500 instructors to determine problem areas/ strength areas/ etc prior to Friday review lecture.

3. Friday review/problem solving session, module week 1 (standard). Review session wrapping up the current weeks material, identifying and providing more instruction and lecture, where appropriate on problem areas identified by practice quiz results. This will be to wrap up and summarize this material prior to moving on to new material in the following Monday lecture

4. Monday lecture, module week 2 (1 hour, 18min). A traditional one-hour, 18 minute lecture at the beginning of the second week of module that covers the remaining material of this module.

5. Student Learning Center Study, week 2 of module. As in week 1. In addition, students will be required to take (supervised) formal online evaluation examinations covering the current module. These evaluations need be completed successfully before the student is able to move on to the following module’s material. These exams should be completed prior to the final Friday lecture of the module. These exams may be repeated, with reduced available point total, for a total of 3 times. If a student does not pass the first two attempts, the student will have to contact TA to discuss potential problems with the module material. Student can make third attempt. If student also does not pass third attempt, s/he may choose to go forward with highest achieved module points, after discussion with TA/faculty. Student will not be able to move to next module w/o passing module test or special permission. We will establish a minimum score for each module to permit progress to the next module. Those students experiencing problems at this point will be identified since the scores and test results will be recorded (and due to the requirement to contact TA or instructor in case of repeated failure to pass). TA support and other instructional help will be offered to students at this time when needed. Results of quizzes and examinations results will be forwarded to MG500 instructors prior to the final Friday lecture of the module to identify problem areas/ strength areas/ etc prior to the second Friday review lecture.

6. Second Friday module review and wrap-up (standard). Review session wrapping up the current module, identifying and providing more instruction and lecture, where appropriate on problem areas identified by practice quiz and examination results. The module will be summarized prior to moving on to the next module on the following Monday

MODULE 1. Rules of Inheritance: Mendelian Genetics: Week 2 and 3

Module subjects -Mendelian genetics -mitosis and meiosis -Sex Linkage and Pedigree Analysis -Extensions of Mendelian genetics -Modifications to Mendelian Patterns of Inheritance -Linkage and Mapping in Eukaryotes

MODULE 2: DNA, Mechanisms of DNA Expression: Week 4 and 5

Module Subjects; -DNA Structure and Chromosome Organization - DNA replication -Transcription -Translation

MODULE 3: Biotechnology

Module Subjects Recombinant DNA Technology -Applications of Recombinant DNA Technologies -Genomics and Bioinformatics

MODULE 4: Regulation of Gene Expression: Week 8 and 9

Module Subjects -Gene Expression: Bacteria and Phages OR -Gene Expression: Eukaryotes -DNA Repais -Mutational Analysis

MODULE 5: Quantitative and Population Genetics: Week 10

Module Subjects - Quantitative Genetics - Population Genetics

Student Directed (Bonus) Module: Weeks 5 to 10

Module Subject -Students will explore a subject of their choice in greater depth, and will be evaluated on that subject at the end of module activity.

Final Exam: Comprehensive Final Exam, 30% of course final grade

COURSE GRADING:

Grades will be based on the point total from: Module Pre-test (minimal points) Module problem sets (minimal points) End of module evaluation quiz (supervised, significant points) End of Student Directed Bonus Module evaluation quiz (supervised, significant points) Comprehensive Final exam (supervised, significant points)

(For detail see spreadsheet) Potential Cost Savings:

Although the primary goal is to enhance learning outcomes and the overall experience for MG500 students, our course redesign plan as currently conceived would result in long term savings in both faculty/instructor time and GTA support. The current six offerings of MG500 per year require 300 hours of faculty lecture time (6 times 50 hrs per offering). The envisioned redesigned MG500 would reduce faculty time to 34 hours per quarter. If the new MG500 replaced the current version of MG500 for three quarters per year, a net saving of 148 hours of faculty lecture time would be realized. If one offering of the traditional MG500 was offered per year along with four quarters of the redesigned MG500, there would be a savings of 98 faculty lecture hours compared to our current offerings.

During the 07/08 academic year, we will utilize ~22 quarters of GTA support (roughly one GTA per every 50 students). Staffing of the MG500 Learning Center with GTAs could be more efficient than our current pattern of GTA utilization. Four to five GTAs could support 50 hours per week of Learning Center availability. GTAs would grade final exams and also be expected to spend time in preparation for assisting students in the learning center, bringing them to a final workload of 20 hours per week. In a model where three quarters of the redesigned MG500 are offered along with one quarter of tradition MG500, we would envision using 16 to 20 quarters of GTA support.

Faculty lecture hours Quarters of GTA support

Current (2007/2008) offering of 6 sections of 300 hours ~22 traditional MG500

Planning/transition year (2008/2009) with 5 sections of traditional and 284 hours 24 1 section of redesigned MG500 2009/2010 with 3 sections of redesigned and 1 152 hours 16 to 20 traditional MG500

2009/2010 with 4 sections of redesigned and 1 186 hours 19 to 25 traditional MG500

Appendix E, Table 1 Molecular Genetics and PCMB Fellowships

Molecular Genetics Fellowships 2007-2008

Postdocs Advisor Dates Fellowship Sponsor

Debies, Michael Weinstein, Michael 06/05-06/08 UoR HCG Postdoctoral Training Award Deminoff, Stephen Herman, Paul 08/05-07/08 T-32, Postdoctoral Cancer Genetics Training Grant National Cancer Institute DeSouza, Colin Osmani, Stephen 07/06-present T-32, Postdoctoral Cancer Genetics Training Grant National Cancer Institute Larson, Jennifer Osmani, Stephen 08/08-present T-32, Postdoctoral Cancer Genetics Training Grant National Cancer Institute Sharma, Nidhi Gustavo Leone 08/08-present T-32, Postdoctoral Cancer Genetics Training Grant National Cancer Institute Trikha, Prashant Gustavo Leone 08/08-present T-32, Postdoctoral Cancer Genetics Training Grant National Cancer Institute

Graduate Student Advisor Dates Fellowship Sponsor

Armstrong, Kristin Chamberlin, Helen 2007 Preparing Future Faculty Fellow Graduate School, OSU Diaz-Munoz, Greetchen Hopper, Anita 2007-present Research Diversity Fellowship NIH Govindaraghavan, Meera Osmani, Stephen 2007 University Fellowship Graduate School, OSU Department of Defense Breast Cancer Program of the Office of the Congressionally Directed Li, Fu Ostrowski, Michael 2009 Predoctoral Traineeship Award Research Programs Malavez, Yadira Doseff, Andrea 2007-present Research Diversity Fellowship NIH Ranji, Arnaz Boris-Lawrie, Kathy 2007 Glenn Barber Endowment Fund Fellowship Riley, Maurisa Cole, Susan 2008-2009 Fellowship American Heart Association Sharma, Amit Boris-Lawrie, Kathy 2008-2010 Predoctoral Fellowship American Heart Association Shifley, Emily Cole, Susan 2007 Herta Camerer Gross Research Fellowship CBS, OSU Stephan, Joseph Herman, Paul 2006-2007 Presidential Fellowship Graduate School, OSU " " 2007-2008 Jeffrey Seilhamer Fellowship OSU Voss, Oliver Doseff, Andrea 2006-2008 Predoctoral Fellowship American Heart Association Williams, Dustin Cole, Susan 2007-2008 Dean's Fellowship CBS, OSU

PCMB Fellowships 2007-2008

Postdocs Advisor Dates Fellowship Sponsor

Aguilar, Isabel Davis, Keith 1998 Posdoctoral Fellowship Spanish Ministry of Science

Graduate Students Advisor Dates Fellowship Sponsor

Li, Hai Yang, Zhenbiao 1999 University Fellowship OSU, graduate school Wolverton, Chris Evans, Michael 1999 ? ? Wang, Ying 2007 PMBB Fellowship OSU, PMBB program Winburn, Jessica Sayre, Richard 2007 PMBB Fellowship OSU, PMBB program Xie, Zidian 2007 PMBB Fellowship OSU, PMBB program Subrahmanian, Nitya Rotating 2008 University Fellowship OSU, graduate school

Appendix F

Metrics D.

3 Masked Tuition Costs

Appendix F, Table 1 pg. 2 - AAU data comparing MG and PCMB to like institutions 0506

health health health total total total tuition tuition Public_ tuition tuition tuition cost cost cost support support support support support inst_name Private Confidentiality Prog_name instate outstate private instate outstate private instate outstate private instate outstate University 1 Public Masked Program 1 4966 9232 1249 1249 23222 29616 6943 13342 University 2 Public Masked Program 2 4966 9232 1249 1249 23618 30012 6943 13342 University 3 Private Masked Program 3 20370 1791 42225 University 4 Public Masked Program 4 6424 17328 0 2736 2736 0 25657 25657 0 6424 6424 University 5 Public Masked Program 5 4951 10471 1075 1075 27026 32546 4951 10471 University 6 Private Masked Program 6 0 0 31100 0 0 631 0 0 56349 0 0 Molecular, Cellular, and University of Colorado at Boulder Public Unmasked Developmental Biology 6965 22445 1500 1500 25761 41241 6030 21510 Cell Biology and Molecular University of Maryland, College Park Public Unmasked Genetics 5633 10757 1352 1352 30376 38062 7074 14760 Molecular, Cellular, Developmental University of Minnesota-Twin Cities Public Unmasked Biology and Genetics 10278 17378 2390 2390 28125 35225 9648 16748 University of Wisconsin-Madison Public Unmasked Genetics 8738 24008 99 99 31883 47153 8738 24008 The Ohio State University Public Unmasked Molecular Genetics 8832 21426 1680 1680 26382 38976 8832 21426 Plant Cellular and Molecular The Ohio State University Public Unmasked Biology Program 8832 21426 1680 1680 25905 38499 8832 21426

Page 1 Masked Tuition Costs

Appendix F, Table 1 pg. 2 - AAU data comparing MG and PCMB to like institutions 0506

health health health total total total tuition tuition tuition health health health tuition tuition tuition cost cost cost support support support support support support support support support Confidentiality Prog_name instate outstate private instate outstate private instate outstate private instate outstate private instate outstate private 1514 1514 1514 1514 20370 900 0 2736 2736 0 1075 1075 31100 0 0 2749

1050 1050

5538 5538

1980 1980 2681 2681 945 945

1260 1260

Page 2 Masked GREs

Appendix F, Table 1 - AAU data comparing MG and PCMB to like institutions 0304 0405 0506

GRE GRE GRE GRE GRE GRE Public_ enrollment GRE verbal quant GRE verbal quant GRE verbal quant inst_name Private Confidentiality Prog_name AU05 takers median median takers median median takers median median University 1 Public Masked Program 1 43 7 460 680 4 545 620 9 590 710 University 2 Public Masked Program 2 37 7 600 730 3 480 730 8 520 695 University 3 Private Masked Program 3 59 10 585 795 8 620 780 11 560 770 University 4 Public Masked Program 4 48 8 460 650 8 540 700 8 530 640 University 5 Public Masked Program 5 10 3 520 770 University 6 Private Masked Program 6 56 9 550 730 7 590 770 5 540 730 Molecular, Cellular, and University of Colorado at Boulder Public Unmasked Developmental Biology 69 10 590 700 9 540 700 18 550 680 Cell Biology and Molecular University of Maryland, College Park Public Unmasked Genetics 74 12 560 715 11 540 710 11 610 680 Molecular, Cellular, Developmental University of Minnesota-Twin Cities Public Unmasked Biology and Genetics 99 15 510 700 16 555 735 29 500 720 University of Wisconsin-Madison Public Unmasked Genetics 86 13 540 730 13 500 720 14 580 720 The Ohio State University Public Unmasked Molecular Genetics 39 9 580 790 8 480 740 4 670 780 Plant Cellular and Molecular The Ohio State University Public Unmasked Biology Program 26 3 460 750 7 440 740 2 550 670

Page 4 Masked

degrees_awarded

Public_ time_to_ inst_name Private Confidentiality Prog_name 0102 0203 0304 0405 0506 degree_0306 University 1 Public Masked Program 1 7 5 8 6 5 5.3 University 2 Public Masked Program 2 4 9 5 7 5 6 University 3 Private Masked Program 3 2 7 8 6 8 5.8 University 4 Public Masked Program 4 4 2 5 4 5 6.3 University 5 Public Masked Program 5 0 1 0 1 3 6 University 6 Private Masked Program 6 3 4 6 8 3 6.32 Molecular, Cellular, and University of Colorado at Boulder Public Unmasked Developmental Biology 3 11 3 9 8 5.97262001 Cell Biology and Molecular University of Maryland, College Park Public Unmasked Genetics 9 6 5 8 9 6 Molecular, Cellular, Developmental University of Minnesota-Twin Cities Public Unmasked Biology and Genetics 5 5 7 12 11 5.3 University of Wisconsin-Madison Public Unmasked Genetics 7 7 7 3 2 6 The Ohio State University Public Unmasked Molecular Genetics 4 6 6 4 7 6 Plant Cellular and Molecular The Ohio State University Public Unmasked Biology Program 1 2 2 1 3 5.75

Page 1 Appendix F, Table 2 ­ Related Genetics departments/graduate programs at AAU Institutions with shared data

Peer unmasked graduate programs: University of Wisconsin Department of Genetics in Arts and Sciences University of Minnesota‐Twin Cities Molecular, Cellular, Developmental Biology and Genetics University of Maryland, College Park Cell Biology and Molecular Genetics University of Colorado at Boulder Molecular, Cellular, and Developmental Biology

Peer masked graduate programs: Michigan State University Genetics/Microbiology and Molecular Genetics Washington University in St. Louis Molecular Genetics (medical school) Stanford University Genetics (medical school) University of Arizona Genetics program (interdisciplinary – cross undergrad and med school) University of Iowa Genetics program (interdisciplinary – cross undergrad and med school)

Universities with similar Genetics departments/programs not sharing information through AAU: University of Georgia Department of Genetics Cornell University Department of Molecular Biology and Genetics Duke University Department of Microbiology and Molecular Genetics (medical school) Princeton University Department of Molecular Biology University of Washington Genome Sciences (integrative undergrad and med school) North Carolina State University Genomic Sciences Graduate Program Penn. State University Interdisciplinary Graduate Program in Genetics Penn. State University State College Biology Department Indiana University Department of Biology Program in Molecular Biology and Genetics Appendix F, Table 3 – Hand-collected metrics data

# # Faculty # Post- # Grad. Stipend Faculty/ Faculty/ Awards docs Students ‘08 Dept. Campus ‘08 OSU MG 14.3 FTE 27 1 AAAS, 1 19 46 $24,552 (21 people) AAM, 1 GSA officer, 1 Royal Society OSU PCMB 7 13 0 15 24 $23,376 U. Colorado 33 33 1 Nobel; 2 71 72 $24,282 AAAS, 2 NAS Cornell 33 33 1NAS, 5 63 57 ? AAAS, 2 AAM U. Georgia 26 40 3 NAS, 1 19 45 $21,000 AAAS U. Maryland 23 23 0? ? ? $25,000 U. Minnesota 56 56 Stanford 34 34 1 Nobel, 2 39 69 ? NAS, 2 AAAS, 1 AAM Washington U. 19 90 1 NAS?, 2 12 ? $27,000 AAAS U. Wisconsin 31 73 5 NAS, 2 ? 65 $22,750 HHMI, 2 GSA pres.

Appendix F, Table 4 OSU/U. Wisconsin detailed comparison

Avg. 2004- AAU 2008 2008 2009 2009 Funding 2006 GRE Report Avg.# PhD Total Research 2008 Avg. Institution Faculty # Service for # PhD (Q/V) AAU Time to award/yr Research Grant $ # # Citations/In Program # FTE Trainers Trainees Students Report Degree 2002-2006 Grant $ Per FTE UnderGrad Postdocs vestigator Avg. # PhD awarded 2002-2006 27 OSU & (37 - all OSU MG 14.9 28 grants 46 770/577 6.00 5.4 $ 3,380,341 $ 245,843 250 trainers) 28.6 OSU & OSU PCMB 6.8 13 UWgrants & 24 720/483 5.75 1.8 $ 2,689,900 $ 395,573 10 12 22.4 U Wisconsin NIH T32 Genetics Med + Basic 32 73 Grants 65 723/540 5.70 5.2 $12,262,839 $437,959 300 ? 50.1

Appendix G

Biosketches MG and PCMB Faculty Program Director/Principal Investigator (Last, First, Middle):

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.

NAME POSITION TITLE Asano, Maki, M.D., Ph.D. Assistant Professor, Ohio State University eRA COMMONS USER NAME (credential, e.g., agency login) EDUCATION/TRAININGASANO01 (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION (if YEAR(s) FIELD OF STUDY applicable) Tohoku University School of Medicine, Sendai, M.D. 1980-86 Medicine Japan Graduate School of Medical Sciences, Kyoto Ph.D. 1987-91 DNA Tumor Viruses University, Japan

A. Positions and Honors.

Positions and Employment 1986-1987 Resident, Department of Pediatrics, Hamamatsu Hospital for Workers’ Accident 1991-1993 Special Cancer Research Fellow, Institute for Virus Research, Kyoto University 1993 Assistant Professor, Institute for Virus Research, Kyoto University 1993-1998 Research Associate, Howard Hughes Medical Institute, Duke University Medical Center (DUMC) 1998-2001 Research Associate, Dept. of Genetics, DUMC 2001-2008 Assistant Research Professor, Dept. of Genetics (Molecular Genetics and Microbiology), DUMC 2008-present Assistant Professor, Tumor Microenvironment Program, James Cancer Cencer, Dept. of Molecular Cellular Biochemistry, College of Medicine, Dept. of Molecular Genetics, College of Biological Science

Other Experience and Professional Memberships 1984-86 Japanese Association for Biochemistry 1984-86 Japanese Association for Immunology 1986-93 Japanese Association for Cancer Research 1986-93 Japanese Association for Molecular Biology 2001- Genetics Society of America

Honors 1980-86 Special Undergraduate Fellowship Japan Ikueikai (Association for the Promotion of Education) 1987-90 Research Fellowship Japan Ikueikai (Association for the Promotion of Education) 1990-93 Special Cancer Research Fellowship (Japanese Association for the Promotion of Science, Special Cancer Research Fund) 1991 Fellowship as a speaker at an International Meeting Inoue Foundation for Science (ICRF, DNA Tumor Virus Meeting) 1991 Fellowship to attend the International Meeting Shimazu Science Foundation (CSH, Eucaryotic DNA Replication Meeting) 1992 Special Cancer Research Fellowship for Collaboration with Hanaoka laboratory (RIKEN) (Japanese Federal Department of Education)

B. Peer-reviewed publications (in chronological order).

PHS 398/2590 (Rev. 11/07) Page Biographical Sketch Format Page Program Director/Principal Investigator (Last, First, Middle):

1. Noda, T., Asano, M., Yoshie, O., Suzuki, R., Ebina, T. and Ishida, N. (1986). Interferon-g induction in human peripheral blood mononuclear cells by OK-432, a killed preparation of Streptococcus pyogenes. Microbiol. Immunol. 30:81-88.

2. Kamachi, Y., Ogawa, E., Asano, M., Ishida, S., Murakami, Y., Satake, M., Ito,Y. and Shigesada, K. (1990). Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomavirus enhancer. J. Virol. 64:4808-4819.

3. Murakami, Y., Asano, M., Satake, M. and Ito,Y. (1990). A tumor promotin phorbor ester, TPA, enhances polyomavirus DNA replication by activating the function of the viral enhancer. Oncogene 5:5-13.

4. Asano, M., Murakami, Y., Furukawa, K., Y.-Iwai, Y., Satake, M. and Ito,Y. (1990). A polyomavirus enhancer-binding protein, PEBP5, responsive to 12-o-tetradecanoylphorbol-13-acetate but distinct from AP-1. J. Virol. l64:5927-5938.

5. Asano, M., Ishikawa, H. and Ito,Y. (1992). Possible involvement of nuclear oncoproteins in regulation of DNA replication. Tohoku J. Exp. Med. 168:183-187.

6. Morgan, I.M., Asano, M., Havarstein, L.S., Ishikawa, H., Hiiragi, T., Ito,Y. and Vogt, P.K. (1993). Amino acid substitutions modulate the effect of Jun on transformation, transcriptional activation and DNA replication. Oncogene 8:1135-1140.

7. Ishikawa, H., Asano, M., Kanda, T., Kumar, S., Gelinas, C. and Ito, Y. (1993). Two novel functions associated with the Rel oncoproteins: DNA replication and cell-specific transcriptional activation and DNA replication. Oncogene 8:2889- 2896.

8. Ito, K., Asano, M., Hughes, P., Kohzaki, H., Masutani, C., Hanaoka, F., Kerppola, T., Curran, T. And Ito, Y. (1996). C- Jun stimulates origin-dependent DNA unwinding by polyomavirus large T antigen. EMBO J. 15:5636-5646.

9. Asano, M., Nevins, J.R. and Wharton, R.P. (1996). Ectopic E2F expression induces S phase and apoptosis in Drosophila imaginal discs. Genes & Development 10:1422-1432.

10. Asano, M. (1999). Regulation of apoptosis and proliferation by E2F – functional analysis in vivo. Experimental Medicine (review issue “Cell cycle and Cancer”) 17: 97-102. In Japanese.

11. Asano, M. and Wharton, Robin P. (1999). E2F mediates developmental and cell cycle regulation of ORC1 in Drosophila. EMBO J. 18: 2435-2448.

12. Burke T.W., Cook J.G., Asano M., Nevins J.R. (2001). Replication Factors MCM2 and ORC1 Interact with the Histone Acetyltransferase HBO1. J. Biol. Chem. 276:15397-15408.

13. Araki M, Wharton RP, Tang Z, Yu H, and Asano M. (2003) Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila. EMBO J. 17; 22: 6115-26.

14. Okudaira K., Ohno K., Yoshida H., Asano M., Hirose F. and Yamaguchi M. (2005) Transcriptional regulation of the Drosophila orc2 gene by the DREF pathway. BBA: Gene Structure & Expression 1732: 23-30.

15. Araki M., Yu H,, and Asano M. (2005) A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo. Genes & Development 19: 2458-2465.

16. Wu Q., Guo Y., Yamada A., Perry JA., Wang MZ., Araki M., Freel CD., Tung JJ., Tang W., Margolis SS., Jackson PK., Yamano H., Asano M., and Kornbluth S. (2007) A role for Cdc2 and PP2A-mediated regulation of Emi2 in the Maintenance of CSF arrest. Curr. Biol. 6; 17(3): 213-24.

17. Narbonned-Reveau K., Senger S., Pal M., Herr A., Asano M., Richardson HE., Deak P. and Lilly MA. (2008) APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle. Development 135: 1451-1461.

18. Park S.Y. and Asano M. The Origin Recognition Complex is dispensable for endoreplication in Drosophila. (2008) PNAS. 105; 34:12343-12348.

PHS 398/2590 (Rev. 11/07) Page2 Continuation Format Page BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Bisaro, David M., Ph.D. Professor of Molecular Genetics eRA COMMONS USER NAME

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Wayne State University, Detroit, MI B.S. 1974 Biology Wayne State University, Detroit, MI Ph.D. 1981 Molecular Biology Imperial College, London, UK Post- 1981-83 Molecular Virology doctorate

A. Positions and Honors. Faculty Appointments 1983-1987 Assistant Professor, Dept. of Botany & Microbiology, Auburn University, Auburn, AL 1987-1991 Assistant Professor, Dept. of Molecular Genetics and Plant Biotechnology Center, OSU 1991-1998 Associate Professor, Dept. of Molecular Genetics and Plant Biotechnology Center, OSU 1998- Professor, Dept. of Molecular Genetics and Plant Biotechnology Center, OSU Other Academic Appointments (The Ohio State University) 1994- Member, Comprehensive Cancer Center 1994- Faculty (non-salaried), Dept. of Plant Pathology 2001- Faculty (non-salaried), Dept. of Molecular Virology, Immunology, and Medical Genetics 2004- Faculty (non-salaried), Dept. of Plant Cellular and Molecular Biology Administrative Appointments (The Ohio State University) 1995- Director, Graduate Program in Molecular, Cellular and Developmental Biology (interdisciplinary, campus-wide program including ~150 faculty and ~120 PhD students) Study Section and Editorial Board Service 1988-1990 SBIR Study Section, National Institutes of Health 1991-1996 Advisory Panel; Eukaryotic Genetics, National Science Foundation 1994-2003 Geminiviridae Classification Study Group, Plant Virology Subcommittee, International Committee for the Taxonomy of Viruses (ICTV) 1995-present Associate Editor: Virology 1996-1999 Advisory Panel; North Carolina Biotechnology Center, Academic Research Initiation Grants 1999-2001 Advisory Panel; North Carolina Biotechnology Center, Collaborative Funding Assistance Program 2006-present Associate Editor: Virus Genes 2008 Advisory Panel; Symbiosis, Defense and Self Recognition, National Science Foundation B. Selected publications (selected from a total of 56) (1998-2008 only) Hartitz, M.D., Sunter, G., and Bisaro, D.M. (1999) The geminivirus transactivator (TrAP) is a single stranded DNA and zinc binding phosphoprotein with an acidic activation domain. Virology 263: 1-14. Sunter, G., Sunter, J., and Bisaro, D.M. (2001) Plants expressing tomato golden mosaic virus AL2 or beet curly top virus L2 transgenes show enhanced susceptibility to virus infection. Virology 285: 59-70. Sunter, G. and Bisaro, D.M. (2003) Identification of a minimal sequence required for activation of the tomato golden mosaic virus coat protein promoter in protoplasts. Virology 305: 452-462. Hao, L., Wang, H., Sunter, G., and Bisaro, D.M. (2003) Geminivirus AL2 and L2 proteins interact with and inactivate SNF1 kinase. Plant Cell 15: 1034-1048. Wang, H., Hao. L.,, Shung, C.-Y., Sunter, G., and Bisaro, D.M. (2003) Adenosine kinase is inactivated by geminivirus AL2 and L2 proteins. Plant Cell 15: 3020-3032. Hong, S.H., Kim, K.I., Chung, H.Y., Kim, Y.J., Sunter, G, Bisaro, D.M., and Chung, I.S. (2004) Expression of recombinant endostatin in Agrobacterium-inoculated leaf disks of Nicotiana tabacum var. Xanthi, Biotechnology Letters 26: 1433-1439. Wang, H., Buckley, K., Yang, X.Y., Buchmann, C., and Bisaro, D.M. (2005) Adenosine kinase inhibition and suppression of RNA silencing by geminivirus AL2 and L2 proteins. Journal of Virology 79: 7410-7418. Bisaro, D.M. (2006) Silencing suppression by geminivirus proteins. Virology 344: 158-168. Kim, K.I., Sunter, G., Bisaro, D.M., and Chung, I.S. (2007) Improved expression of recombinant GFP using a replicating vector based on Beet curly top virus in leak disks and infiltrated Nicotiana benthamiana leaves. Plant Molecular Biology 64: 103-112. Yang, X., Baliji, S., Buchmann, R.C., Wang, H., Lindbo, J., Sunter, G., and Bisaro, D.M. (2007) Functional modulation of the geminivirus AL2 transcription factor and silencing suppressor by self-interaction. Journal of Virology 81: 11972-11981. Raja, P., Sanville, B.C., Buchmann, R.C., and Bisaro, D.M. (2008) Viral genome methylation as an epigenetic defense against geminiviruses. Journal of Virology 82: 8997-9007. Buchmann, R.C., Asad, S., Mohannath, G., and Bisaro, D.M. (2008) Geminivirus AL2 and L2 proteins reverse transcriptional gene silencing and cause genome-wide reductions in cytosine methylation. Journal of Virology. Accepted pending minor revision. Golenberg, E.M., Sather, D.N., Hancock, L.C., Buckley, K.J., and Bisaro, D.M. (2008) Development of a gene silencing DNA vector derived from a broad host range geminivirus. In preparation. C. Research Support. Projects Ongoing Viral Chromatin Methylation as a Host Defense Against Geminiviruses (05/08 - 04/12) NSF; Symbiosis, Defense and Self-Recognition Program (MCB-0743261) PI: David M. Bisaro This project investigates the hypothesis that plants use methylation as a defense against DNA viruses, and that DNA viruses produce proteins that can inhibit methylation and transcriptional gene silencing. The Role of Adenosine Kinase in Metabolic Defense Signaling (04/07 - 03/09) Ohio Agricultural Research and Development Center (OARDC) Seed Grant Program (2007-044) PI: David M. Bisaro This project studies the role of adenosine kinase in antiviral signaling leading to the cellular stress response. Characterization of Ebola virus VP35 RNA silencing activity and targets (03/08 – 02/09) NIH/NIAID Region V GLRCE Development Projects (60014886) PI: Deborah S. Parris/ Co-PI: David M. Bisaro This project seeks to establish that VP35 is a suppressor of RNA interference pathways, to identify regions of the protein required for silencing activity, and to identify specific small RNA activities affected by VP35. Projects pending: Mechanism of RNA silencing suppression by Ebola virus pathogenicity factor VP35 NIH, NIAID (R21) PI: Deborah S. Parris/ CoPI: David M. Bisaro The aim of this project is to determine the mechanisms by which EBOV VP35 suppresses RNA silencing in mammalian cells. Mechanism of US11 in RNA-induced silencing suppression in Herpes Simplex Virus NIH, NIAID (R01 AI077636-01A1) PI: Deborah S. Parris/ CoPI: David M. Bisaro This aim of this project is to determine if RNA interference is used by mammalian cells as a defense against HSV, and to elucidate the mechanisms by which HSV US11 protein can suppress this defense. Projects completed (last three years): Title: Molecular Mechanisms of Geminivirus Replication (09/04 - 08/08) USDA; National Research Initiative, Plant Genetics and Molecular Biology Program (2004-35301-14508) PI: David M. Bisaro This project was concerned with the mechanisms by which geminivirus AL2 protein activates transcription of the viral coat protein promoter, and tested the hypothesis that AL2 suppresses RNA silencing by inhibiting cellular methylation pathways. Title: Suppression of RNA Interference by Herpes Simplex Virus (07/05 - 06/08) NIH, NIAID (R21 AI062837-02) PI: Deborah S. Parris/ CoPI: David M. Bisaro This aim of this project was to determine if RNA interference is used by mammalian cells as a defense against HSV, and whether HSV can suppress this defense.

Program Director/Principal Investigator (Last, First, Middle):

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Gregory C. Booton Research Associate/ Faculty Instructor eRA COMMONS USER NAME (credential, e.g., agency login)

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) The Ohio State University B.S. 1988 Molecular Genetics The Ohio State University Ph.D. 1995 Molecular Genetics The Ohio State University Post-doc 1995-1998 Molecular Evolution

A. Positions and Honors.

1/05-present Faculty Teaching Instructor, Department of Molecular Genetics, OSU 6/03-present Research Associate, Department of Evolution, Ecology, and Organismal Biology, OSU 3/98-5/03 Research Associate, Department of Molecular Genetics, OSU 2/95-2/98 Post-Doctoral Researcher, Department of Molecular Genetics, OSU

B. Selected peer-reviewed publications (in chronological order).

G. C. Booton, C. E. Joslin, M. E. Shoff, E. Y. Tu, D. J. Kelly, & P. A. Fuerst. GENOTYPIC IDENTIFICATION OF ACANTHAMOEBA SP ISOLATES ASSOCIATED WITH AN OUTBREAK OF ACANTHAMOEBA KERATITIS (AK) (Accepted, Cornea, 10/08). R. Sriram, M. Shoff, G. Booton, P. Fuerst, & G. S. Visvesvara. ACANTHAMOEBA SPP. CYSTS SURVIVE DESICCATION FOR MORE THAN 20 YEARS. (In Press, J Clin Micro, 10/08) Tu, E. Y., C. E. Joslin, J. S. Sugar, M. E. Shoff , & G. C. Booton. PROGNOSTIC FACTORS AFFECTING VISUAL OUTCOME IN ACANTHAMOEBA KERATITIS. (In Press, Ophthamology, 10/08) Tu, E. Y., C. E. Joslin, J. S. Sugar, G. C. Booton, M. E. Shoff. 2008. THE RELATIVE VALUE OF CONFOCAL MICROSCOPY AND SUPERFICIAL CORNEAL SCRAPINGS IN THE DIAGNOSIS OF ACANTHAMOEBA KERATITIS. Cornea, 27:764-772. Seal, D., U. Player; Contributing Authors: G. Booton, C. Ferrer, S. Gardner, R. Kowalski, & P. Thomas. 2007. OCULAR INFECTION, CRC Press, ISBN 0849380931, 384pp. C. Joslin, E. Tu, M. Shoff, G. Booton, P. Fuerst, T. McMahon, R. Anderson, M. Dworkin, J. Sugar, F. Davis, & L. Stayner. 2007. THE ASSOCIATION OF CONTACT LENS SOLUTION USE AND ACANTHAMOEBA KERATITIS. Am J Ophthalmol 2007;144:169–180. R. Walia, J. G. Montoya, G. S. Visvesvara, G. C. Booton, R. L. Doyle. 2007. A CASE OF SUCCESSFUL TREATMENT OF CUTANEOUS ACANTHAMOEBA INFECTION IN A LUNG TRANSPLANT RECIPIENT Transpl Infect Dis 9:51-54 Kinde, D. H. Read, B.M. Daft, M. Manzer, R. W. Nordhaussen, D. J. Kelly, P. A. Fuerst, G. Booton, G. S. Visvesvara 2007. INFECTIONS CAUSED BY PATHOGENIC FREE- LIVING AMOEBAE (BALAMUTHIA MANDRILLARIS AND ACANTHAMOEBA SP.) IN HORSES. H. J Vet Diagn Invest 19:317-322. McKellar, M., L. R. Mehta, J. E. Greenlee, D. C. Hale, G. C. Booton, D. J. Kelly, P. A. Fuerst, R. Sriram, and G. S. Visvesvara. 2006. FATAL GRANULOMATOUS ACANTHAMOEBA ENCEPHALITIS MIMICKING A STROKE, DIAGNOSED BY CORRELATION OF RESULTS OF SEQUENTIAL MAGNETIC RESONANCE IMAGING, BIOPSY, IN VITRO CULTURE, IMMUNOFLUORESENCE ANALYSIS, AND

PHS 398/2590 (Rev. 11/07) Page Biographical Sketch Format Page Program Director/Principal Investigator (Last, First, Middle): PI Name MOLECULAR ANALYSIS. J. Clin. Micro. 44: 4265-69. Visvesvara, G. S., G. Booton, D. J. Kelly, P. Fuerst, R. Sriram, A. Finkelstein, & M. M. Garner. 2006. IN VITRO CULTURE, SEROLOGIC AND MOLECULAR ANALYSIS OF ACANTHAMOEBA ISOLATED FROM THE LIVER OF A KEEL-BILLED TOUCAN (RAMPHASTOS SULFURATUS) Vet Parisitol, 143:74-8. Yagi, S., G. C. Booton, G. S. Visvesvara. 2005. DETECTION OF BALAMUTHIA MITOCHONDRIAL 16S RIBOSOMAL DNA IN CLINICAL SPECIMENS BY THE POLYMERASE CHAIN REACTION. Journal of Clinical Microbiology 43:3192-3197. Dubey, J. P., J. E. Benson, K. T. Blakely, G. C. Booton, & G. S. Visvesvara. 2005. DISSEMINATED ACANTHAMOEBA SP. INFECTION IN A DOG. Veterinary Parasitology, 128: 183-187. Booton, G. C., G. S. Visvesvara, T. J. Byers, D. J. Kelly, & P. A. Fuerst. 2005. DIFFERENTIAL DISTRIBUTION OF ACANTHAMOEBA SPP. GENOTYPES IN ACANTHAMOEBA KERATITIS (AK) AND NON-KERATITIS INFECTIONS: IMPLICATIONS FOR POTENTIAL PATHOGENICITY. Journal of Clinical Microbiology, 43:1689-1693. Booton, G. C., G. L. Floyd, & P. A. Fuerst. 2004. MULTIPLE GROUP I INTRONS IN THE NUCLEAR SMALL SUBUNIT rDNA OF THE AUTOSPORIC GREEN ALGA SELENASTRUM CAPRICORNUTUM. Current Genetics 46:228-234. Dunnebacke, T. H., F. L. Schuster, S. Yagi, & G. C. Booton. 2004. BALAMUTHIA MANDRILLARIS FROM SOIL SAMPLES. Microbiology 150(pt. 9):2837-2842. Booton G. C., A. Rogerson, T. D. Bonilla, D. V. Seal, D. J. Kelly, T. K. Beattie, A. Tomlinson, F. Lares-Villa, P. A. Fuerst, & T. J. Byers. 2004. MOLECULAR AND PHYSIOLOGICAL EVALUATION OF SUBTROPICAL ENVIRONMENTAL ISOLATES OF ACANTHAMOEBA SPP., CAUSAL AGENT OF ACANTHAMOEBA KERATITIS. Journal of Eukarotic Microbiology 51:192-200. Booton, G. C., J. R. Carmichael, G. S. Visvesvara, T. J. Byers, & P. A. Fuerst. 2003. PCR IDENTIFICATION OF BALAMUTHIA MANDRILLARIS USING THE MITOCHONDRIAL 16S rRNA GENE AS A TARGET. Journal of Clinical Microbiology, 41:453-455. Booton G. C., J. R. Carmichael, G. S. Visvesvara, T. J. Byers, P. A. Fuerst. 2003. GENOTYPING OF BALAMUTHIA MANDRILLARIS BASED ON NUCLEAR 18S AND MITOCHONDRIAL 16S rRNA GENES. American Journal of Tropical Medicine and Hygiene, 68:65-69. Ledee, D. R., G. C. Booton, M. H. Awwad, S. Sharma, R. K., I. A. Niszl, M. B. Markus, P. A. Fuerst, & T. J. Byers. 2003. ADVANTAGES OF USING MITOCHONDRIAL 16S rDNA SEQUENCES TO CLASSIFY CLINICAL ISOLATES OF ACANTHAMOEBA. Investigative Ophthalmology and Visual Science, 44:1142-1149. Schuster, F. L., Dunnebacke, T. H., Booton, G. C., Yagi, S., Kohlmeier, C. K., Glaser, C., Vugia, D., Bakardjiev, A., Azimi, P., Maddux-Gonzalez, M., Martinez, A. J., & Visvesvara, G. S. 2003. ENVIRONMENTAL ISOLATION OF BALAMUTHIA MANDRILLARIS ASSOCIATED WITH A CASE OF AMEBIC ENCEPHALITIS. Journal of Clinical Microbiology, 41:3175–3180.

C. Research Support. Ongoing Research Support RO1 EY09073 1/1/05-12/31/08 NEI DNA Probes for Acanthamoeba Role: Co-PI

PHS 398/2590 (Rev. 11/07) Page 2 Continuation Format Page BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Helen M. Chamberlin Associate Professor eRA COMMONS USER NAME helen_chamberlin EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Utah B.S. 1985-1988 Psychology California Institute of Technology Ph.D. 1988-1994 Biology Simon Fraser University Postdoctoral 1994-1995 Molecular Biology University of Washington Postdoctoral 1995-1999 Genetics

Please refer to the application instructions in order to complete sections A, B, and C of the Biographical Sketch.

A. Professional Employment 1999-2005 Assistant Professor, Ohio State University, Department of Molecular Genetics 2005-present Associate Professor, Ohio State University, Department of Molecular Genetics

Honors, Awards, and Committees 1985-1988 National Merit with Presidential Honors Scholorship 1988 Summa cum laude, University of Utah 1988-1991 National Science Foundation Graduate Fellowship 1994 Ferguson Thesis Prize, California Institute of Technology 1994-1997 Helen Hay Whitney Postdoctoral Fellowship 2001-2006 NSF CAREER Award 2003-2006 Member, NCI IRG-F: Training & Manpower grant proposal committee 2004 Dean's Award for Excellence in Research and Graduate Education 2005-present Member, Faculty of 1000, Developmental Biology Group

B. Research Publications Okimoto, R., Chamberlin, H. M., Macfarlane, J. L. and Wolstenholme, D. R. (1991) Repeated sequence sets in mitochondrial DNA molecules in root knot nematodes (Meloidogyne): nucleotide sequences, genome location and potential for host-race identification. Nucleic Acids Res., 19, 1619-1626. Chamberlin, H. M. and Sternberg, P. W. (1993) Multiple cell interactions are required for fate specification during male spicule development in Caenorhabditis elegans. Development, 118, 297-324. Chamberlin, H. M. and Sternberg, P. W. (1994) The lin-3/let-23 pathway mediates inductive signalling during male spicule development in Caenorhabditis elegans. Development, 120, 2713-2721. Chamberlin, H. M. and Sternberg, P. W. (1995) Mutations in the Caenorhabditis elegans gene vab-3 reveal distinct roles in fate specification and unequal cytokinesis in an asymmetric cell division. Developmental Biology, 170, 679-689. Chamberlin, H. M., Palmer, R. E., Newman, A. P., Sternberg P. W., Baillie, D. L. and Thomas, J. H. (1997) The PAX gene egl-38 mediates developmental patterning in Caenorhabditis elegans. Development, 124, 3919-3928. Stewart, H. I., O'Neil, N. J., Janke, D. L., Franz, N. W., Chamberlin, H. M., Howell. A. M., Gilchrist, E. J., Ha, T. T., Kuervers, L. M., Vatcher, G. P., Danielson, J. L. and Baillie, D. L. (1998) Lethal mutations defining 112 complementation groups in a 4.5 megabase sequenced region of Caenorhabditis elegans chromosome III. Mol. & Gen. Genetics, 260, 280-288. Chamberlin, H. M., Brown, K. B., Sternberg, P. W. and Thomas, J. H. (1999) Characterization of seven genes affecting Caenorhabditis elegans hindgut development. Genetics, 153, 731-742. Chamberlin, H. M. and Thomas, J. H. (2000) The bromodomain protein LIN-49 and Trithorax-related protein LIN-59 affect development and gene expression in Caenorhabditis elegans. Development, 127, 713- 723. Johnson, A. D., Fitzsimmons, D., Hagman, J. and Chamberlin, H. M. (2001) EGL-38 Pax regulates the ovo- related gene lin-48 during Caenorhabditis elegans organ development. Development, 128, 2857-2865. Fitzsimmons, D., Lutz, R., Wheat, W., Chamberlin, H. M. and Hagman, J. (2001) Highly conserved amino acids in Pax and Ets proteins are required for DNA binding and ternary complex assembly. Nucleic Acids Res., 29, 4154-4165. Wang, X. and Chamberlin, H. M. (2002) Multiple regulatory changes contribute to the evolution of the Caenorhabditis lin-48 ovo gene. Genes & Development, 16, 2345-2349. Sewell, S.T., Zhang, G., Uttam, A. and Chamberlin H.M. (2003) Developmental patterning in the Caenorhabditis elegans hindgut. Developmental Biology, 262, 88-93. Wang, X. and Chamberlin, H.M. (2004) Evolutionary innovation of the excretory system in Caenorhabditis elegans. Nature Genetics, 36, 231-232. Wang, X., Greenberg, J.F. and Chamberlin, H.M. (2004) Evolution of regulatory elements producing a conserved gene expression pattern in Caenorhabditis. Evolution & Development, 6, 237-245. Zhang, G., Tseng, R.-J., Rajakumar, V., Wang. X. and Chamberlin, H.M. (2005) Alteration of the DNA binding domain disrupts tissue-specific functions of the C. elegans Pax protein EGL-38. Mechanisms of Development, 122, 887-899 Wang, X., Jia, H. and Chamberlin, H.M. (2006) The bZip proteins CES-2 and ATF-2 alter the timing of transcription for a cell-specific target gene in C. elegans. Developmental Biology, 289, 456-465. Park, D., Jia, H., Rajakumar, V. and Chamberlin, H.M. (2006) Pax2/5/8 proteins promote cell survival in C. elegans. Development, 133, 4293-4302. Rajakumar, V. and Chamberlin, H.M. (2007) The Pax2/5/8 protein EGL-38 plays multiple roles to coordinate organogenesis of the C. elegans egg-laying system. Developmental Biology, 301, 240-253. Tseng, R.-J., Armstrong, K.R., Wang, X. and Chamberlin, H.M. (2007) The bromodomain protein LEX-1 acts with TAM-1 to modulate gene expression in C. elegans. Molecular Genetics and Genomics, 278, 507-518. Mah, A.K., Armstrong, K.R., Chew, D.S., Chu, J.S., Tu, D.K., Johnson, R.C., Chen, N.S., Chamberlin, H.M., and Baillie, D.L. (2007) Transcriptional regulation of aqp-8, a Caenorhabditis elegans aquaporin exclusively expressed in the excretory system, by the POU homeobox transcription factor CEH-6. Journal of Biological Chemistry, 282, 28074-28086. Sun, H., Nelms, B.L., Sleiman, S.F., Chamberlin, H.M. and Hanna-Rose, W. (2007) Modulation of C. elegans transcription factor activity by HIM-8 and the related zinc finger ZIM proteins. Genetics, 177, 1221-1226. Johnson, R.W. and Chamberlin, H.M. (2008) Positive and negative regulatory inputs restrict pax-6/vab-3 transcription to sensory organ precursors in C. elegans. Mechanisms of Development, 125, 486-497. Jia, H., Johnson, R.W., and Chamberlin, H.M. The C. elegans Pax2/5/8 protein EGL-38 confers susceptibility to infection and promotes expression of predicted immune effectors. Manuscript under revision. Armstrong, K.R. and Chamberlin, H.M. Coordinate regulation of functionally related genes in the excretory organ of C. elegans by the POU domain protein CEH-6. Manuscript under revision. Li, X., Kulkarni, R.P., Hill, R.J., and Chamberlin, H.M. Integration of HOM-C gene activity with WNT and EGF signaling in C. elegans P12 cell development. Manuscript under revision. Johnson, R.W., Liu, L., Hanna-Rose, W. and Chamberlin, H.M. The C. elegans heterochronic gene lin-14 coordinates temporal progression and maturation in the egg-laying system. Manuscript under revision.

BIOGRAPHICAL SKETCH

NAME POSITION TITLE Tien-Hsien Chang Associate Professor

EDUCATION /TRAINING(Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION DEGREE YEAR(S) FIELD OF STUDY (if applicable) National Taiwan University B.S. 1979 Botany State University of New York at Buffalo M.A. 1983 Molecular Biology State University of New York at Buffalo Ph.D. 1986 Molecular Biology

Professional Experience 1981 - 1986 Graduate Research Assistant, State Univ. of New York at Buffalo, Buffalo, New York 1986 - 1991 Postdoctoral Fellow, California Institute of Technology, Pasadena, California 1992 - 1997 Assistant Professor, Ohio State University, Columbus, Ohio 1997 - Present Associate Professor, Ohio State University, Columbus, Ohio 1992 - Present Member, Molecular, Cellular, and Developmental Biology Program, Ohio State Univ.

Honors and Awards 1984-1986 Genetic Graduate Group Predocotoral Fellowship, State Univ. New York, Buffalo 1988-1991 Merck Sharp & Dohme Laboratories Postdoctoral Fellowship 1991-1992 OSU Comprehensive Cancer Center Starter Cancer Research Award: “Studies on the functions of yeast PRP11 protein” 1991-1992 American Cancer Society Ohio Division Beginning Investigator Award: “Investigation of the functions of three putative RNA helicases involved in yeast mRNA splicing” 1992-1993 OSU University Seed Grant: “Studies of RNA helicases involved in yeast pre-mRNA splicing” 1993-1995 NIH Shannon Award: “Studies of RNA helicases involved in yeast mRNA splicing” 1995-1996 NSF Small Grant for Exploratory Research (SGER): “The yeast 3-hybrid system: a genetic method for screening RNA-protein interactions” 1996-1997 American Cancer Society Ohio Division Research Grant: “Studies of a novel translation initiation factor, the yeast DED1 protein” 1994-1999 NIH FIRST (R29) Award: “Studies of RNA helicases involved in yeast mRNA splicing” 2000-2003 NSF Grant "Regulation of U1 snRNP/5' splice site interactions during pre-mRNA splicing" 2005 Dean's Award for Classroom Teaching, College of Biological Sciences, Ohio State University

Publications

1. Tsai, H.-K., Chou, M.-Y., Shih, C.-H., Huang, G. T.-W., Chang, T.-H. and Li, W.-H. (2007) MYBS: a comprehensive web server for mining transcription factor binding sites in yeast. Nucl. Acids. Res. 1-6. doi: 10.1093/nar/gkm379 2. Kapadia, F., Pryor, A., Chang, T.-H., Johnson, L. F. (2006) Nuclear localization of poly(A)+ mRNA following siRNA reduction of expression of the mammalian RNA helicases UAP56 and URH49. Gene, 384, 37-44. 3. Burckin, T., Nagel, R., Mandel-Gutfreund, Y., Shiue, L., Clark, T., Chong, J.-L., Chang, T.-H., Squazzo, S., Hartzog, G., and Ares, M., Jr. (2005) Exploring functional relationships between components of the transcription, splicing, and mRNA export machineries by gene expression phenotype analysis. Nature Struct. Mol. Biol. 12, 175-182. 4. Chong,, J.-L., Chuang, R.-Y., Tung, L., and Chang, T.-H. (2004) Ded1p, a conserved DExD/H-box translation factor, can promote L-A virus negative-strand RNA synthesis in vitro. Nucl. Acids Res. 32, 2031-2038. 5. Pryor, A., Tung, L., Yang, L., Kapadia, F., Chang, T.-H., and Johnson, L. F. (2004) Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExD/H-box protein that is highly related to the mRNA export protein UAP56. Nucl. Acids Res. 32, 1857-1865. 6. Tseng-Rogenski, S. S.-I, Chong, J.-L., Thomas, C. B., Enomoto, S. Berman, J., and Chang, T.-H. (2003) Functional conservation of Dhh1p, a DExD/H-box protein in Saccharomyces cerevisiae. Nucl. Acids Res. 31, 4995-5002. 7. Chen, J. Y.-F., Stands, L., Staley, J. P., Jackups, Jr., R. R., Latus, L. J., and Chang, T.-H. (2001) Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD-box splicing factor. Mol. Cell. 7, 227-232. 8. Tsai-Morris, C. H., Lei, S., Jiang, Q., Chang, T.-H., and Dufau, M. L. (2001) Genomic structure, phylogenetic analysis and functional studies of the mouse RNA helicase DDX25/GRTH gene FASEB J. 15, A880-A880 9. Tseng, S. S.-I, Weaver, P. L., Liu, Y., Hitomi, M., Tartakoff, A. M., and Chang, T.-H. (1998) A cyotosolic RNA helicase required for poly(A)+ RNA export. EMBO J., 17, 2651-2662. 10. Chuang, R.-Y., Weaver, P. L., Liu, Z., and Chang, T.-H. (1997) Requirement of the DEAD-box protein Ded1p for messenger RNA translation. Science, 275, 1468-1471. 11. Weaver, P. L., Sun, C., and Chang, T.-H. (1997). Dbp3p, a putative RNA helicase in Saccharomyces cerevisiae, is required for efficient pre-ribosomal RNA processing predominantly site A3. Mol. Cell. Biol., 17, 1354-1365. 12. Chang, T.-H., Latus, L. J., Liu, Z., and Abbott, J. (1997) Genetic interactions of conserved regions in the DEAD-box protein Prp28p. Nucl. Acids Res., 25, 5033-5040. 13. Liu, Y., Chang, T.-H., and Tartakoff, A. (1997) Mechanisms of transport of hnRNP-like proteins. Mol. Biol. Cell 8, 1370. 14. Ruby, S. W., Chang, T.-H., and Abelson, J. (1993). Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA, Genes Dev., 7, 1909-1925. 15. Chang, T.-H. and Baum, B. (1992). Mapping the putative RNA helicase genes by sequence overlapping. Yeast, 8, 973-975. 16. Chang, T.-H. and Abelson, J. (1990) Identification of a putative amidase gene in yeast Saccharomyces cerevisiae, Nucl. Acids Res., 18, 7180. 17. Chang, T.-H., Arenas, J., and Abelson, J. (1990) Identification of five putative yeast RNA helicase genes. Proc. Natl. Acad. Sci. USA, 87, 1571-1575. 18. Chang, T.-H., Clark, M. W., Lustig, A. J., Cusick, M. E., and Abelson, J. (1988) RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus. Mol. Cell. Biol., 8, 2379-2393. 19. Chang, T.-H., Banerjee, N., Bruenn, J., Held, W., Peery, T., and Koltin, Y. (1988) A very small viral double-stranded RNA. Virus Genes, 2, 147-158. 20. Chang, T.-H. and Abelson, J. (1987) RNA11 gene product and the spliceosome. Mol. Biol. RP, 12, 211. 21. Field, L., Bruenn, J., Chang, T.-H., Pinhasi, O., and Koltin, Y. (1983) Two Ustilago maydis dsRNAs of different sizes code for the same product. Nucl. Acids Res., 11, 2756-2778.

Manuscripts Submitted or In Preparation

1. Tung, L. and Chang, T.-H. (2008) Functional coupling of transcription and splicing through intron branch site recognition in Saccharomyces cerevisiae. Submitted to Genes Dev. 2. Hage, R., Tung, L., and Chang, T.-H. (2008) Role of Ynl187p, a novel splicing factor, in promoting the U1 snRNP and 5’ splice site interaction. Submitted to Mol. Cell. Biol. 3. Shih, C.-H., Tsai. Y.-F., Su, C.-H., Chang, T.-H., and Tsai, H.-K. (2008) Limited portion of divergently transcribed gene pairs share regulatory modules in yeast. Submitted to BMC Genomics.

Book Chapter

1. Tseng-Rogenski, S. S.-I and Chang, T.-H. RNA unwinding assay for DExD/H-box RNA helicases. In Schoenberg,, D. R. (ed), Methods in Molecular Biology: mRNA Processing and Metabolism. Humana. 2004.

PI: Susan E. Cole

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Susan E. Cole eRA COMMONS USER NAME Assistant Professor of Molecular Genetics COLE354 EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) The University of Rochester, Rochester NY B.S. 1991 Molecular Genetics The Johns Hopkins University, Baltimore, MD Ph.D. 1998 Human Genetics Princeton University, Princeton NJ Postdoctoral 1998-2003 Developmental Biology

A. Positions and Honors. Professional Experience 1998-2003 Postdoctoral Fellow, Princeton University, Princeton, NJ. Advisors Thomas F. Vogt and Shirley M. Tilghman 2003-present Assistant Professor of Molecular Genetics, Ohio State University, Columbus, OH 2005-present Associate Member, Ohio State Comprehensive Cancer Center. Honors and Awards 1987-1991 University of Rochester National Merit Scholar 1987-1991 University of Rochester Bausch and Lomb Award 1999-2002 NIH Individual National Research Service Award 2005-2007 March of Dimes Basil O’Connor Starter Scholar Research Award

B. Publications Lazebnik Y.A., S. Cole, C.A. Cooke, W.G. Nelson, W.C. Earnshaw. (1993) Nuclear events of apoptosis in vitro in cell-free mitotic extracts: a model system for analysis of the active phase of apoptosis. J Cell Biol 23:7-22. Mjaatvedt A.E., D.E. Cabin, S.E. Cole, L.J. Long, G.E. Breitwieser, R.H. Reeves. (1995) Assessment of a mutation in the H5 domain of Girk2 as a candidate for the weaver mutation. Genome Res 5:453-463. Cole, S.E., T. Wiltshire, R.H. Reeves. (1998) Physical mapping of the evolutionary boundary between human Chromosomes 21 and 22 on mouse Chromosome 10. Genomics 50: 109-111. Cole, S.E., and R.H. Reeves. (1998) A cluster of keratin associated proteins on MMU10 in the region of conserved linkage with HSA 21. Genomics 54:437-442. Cole, S.E., T. Wiltshire, E.E. Rue, D. Morrow, P. Hieter, C. Brahe, N. Katsanis, E.M. Fisher, R.H. Reeves. (1999) High resolution physical and comparative mapping of mouse Chromosome 10 in a region of conserved synteny with human Chromosome 21. Mammalian Genome 10:229-234. Wiltshire T., M. Pletcher, S.E. Cole, M. Villanueva, B. Birren, J. Lehoczky, K. Dewar, R.H. Reeves. (1999) Perfect conserved linkage across the entire mouse Chromosome 10 region homologous to human Chromosome 21. Genome Res 9:1214-1222. Bohne, J., S.E. Cole, C. Sune, B.R. Lindman, V.D. Ko, T.F. Vogt, M.A. Garcia-Blanco. (2000) Expression analysis and mapping of the mouse and human transcriptional regulator CA150. Mammalian Genome 11:930-933. Cole, S.E., M.S. Mao, S.H. Johnston, T.F. Vogt. (2001) Identification, expression analysis and mapping of

1 PI: Susan E. Cole B3galt6, a putative galactosyltransferase with similarity to Drosophila brainiac. Mammalian Genome 12:177-179. Bendotti C, S.E. Cole, M. Gobbi, C. Hohmann and R.H. Reeves. (2002) Overexpression of S100ß in transgenic mice does not protect from serotonergic denervation induced by 5,7-dihydroxytryptamine (5,7- dht). J Neurosci Res. 67:501-510. Cole, S.E., J.M. Levorse, S.M. Tilghman, T.F. Vogt. (2002). Clock regulatory elements control cyclic expression of Lunatic fringe during somitogenesis. Dev Cell 3: 75-84. Shifley, E.T. and Cole, S.E. (2007). The vertebrate segmentation clock and its role in skeletal birth defects. Birth Defects Res C Embryo Today. 81:121-133. Shifley, E. T., K. M. Vanhorn, A. Perez-Balaguer, J. D. Franklin, M. Weinstein and S. E. Cole (2008) Oscillatory lunatic fringe activity is crucial for segmentation of the anterior but not posterior skeleton. Development 135, 899-908. McBride, K.L., M.F. Riley, G.A. Zender, S.M. Fitzgerald-Butt, J.A. Towbin, J.W. Belmont and S.E. Cole. (2008) Notch1 mutations in individuals with left ventricular outflow tract malformations reduce ligand- induced signaling. Hum Mol. Gen. 17, 2886-93. Shifley, E.T. and S.E. Cole. (2008) Lunatic fringe protein processing by proprotein convertases may contribute to the short protein half-life in the segmentation clock. Biochim Biophys Acta, Mol Cell Res. July 25. [Epub ahead of print] Ryan, M.J., C. Bales, A. Nelson, D.M. Gonzalez, L. Underkoffler, M. Segalov, J. Wilson-Rawls, S.E. Cole, J.L. Moran, P. Russo, N.B. Spinner, K. Kusumi, and K.M. Loomes. (2008) Bile Duct Proliferation in Jag1/Fringe Heterozygous Mice Identifies Candidate Modifiers of the Alagille Syndrome Hepatic Phenotype. Hepatology, in press.

C. Research Support (Recent and Ongoing) Title: Dissecting the roles of Lunatic fringe and Notch signaling during vertebrate somitogenesis Agency: March of Dimes 06/01-2008- 05/31/2011 The Long term objectives of this project are to understand how NOtch signaling and the segmentation clock are differentially regulated during primary and secondary body formation. Title: Regulation of Lunatic fringe protein activity and its modulation of Notch signaling Agency: American Cancer Society, Ohio Division 1/1/06-12/31/06 This pilot grant was awarded to generate preliminary data with the long term objective of analyzing the mechanisms by which LFNG protein activity is regulated post-translationally. Title: Modulation of Lunatic fringe gene activity and Notch signaling in the segmentation clock Agency: Ohio Cancer Research Associates 07/01/2005-06/30/2007 This pilot grant was awarded to generate prliminary data with the long term objective of analyzing the role of the Lfng 3’UTR in the post-transcriptional regulation of RNA stability and translational efficiency. Title: Defining Roles of Notch Signaling During Vertebrate Segmentation Agency: March of Dimes 02/01/05-01/31/07 The long term objective of this project is to examine the distinct roles of Notch signaling during somitogenesis both within the segmentation clock and during AP patterning of the developing somites. The goals are to generate mouse models with Notch signaling perturbed during specific aspects of segmentation. Title: A cell culture model of a Notch-based cellular clock. Agency: Ohio State University Clinical Cancer Center 10/31/03-9/30/04 The major goal of this project is to establish and analyze a tissue culture model of the Notch-based segmentation clock.

2

Principal Investigator/Program Director (Last, First, Middle): Ding, Biao

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Biao Ding Professor of Plant Cellular and Molecular Biology

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Beijing Forestry University B.S. 1982 Forestry Cornell University M.S. 1986 Plant Anatomy Cornell University Ph.D. 1991 Plant Cell Biology University of California, Davis Postdoc 1991-1994 Plant Cell Biology

A. Positions and Honors Professional Positions 1994-1999 Assistant Professor, Department of Botany, Oklahoma State University, OK 1999-2000 Associate Professor, Department of Botany, Oklahoma State University, OK 2000-2005 Associate Professor, PCMB and Plant Biotechnology Center, Ohio State University, OH 2005- Professor, PCMB and Plant Biotechnology Center, OSU, OH Other Experience and Professional Memberships Member, American Society for Virology Member, American Society for Microbiology Member, International Society of Molecular Plant-Microbe Interactions Member, American Society of Plant Biologists Honors 1983-1985 Graduate Fellowship for Overseas Studies, the Chinese Government 1999 The College of Arts and Sciences Faculty Council Junior Faculty Award for Scholarly Excellence, Oklahoma State University 2005 Harlan Hatcher Memorial Award for Excellence, Colleges of Arts and Sciences, OSU 2005-2007 Outstanding Overseas Youth Research Award, National Science Foundation of China B. Selected Peer-Reviewed Publications (Selected from 42 peer-reviewed research papers) 1. Itaya, A., Woo, Y.-M., Masuta, C., Bao, Y., Nelson, R., and Ding, B. (1998). Developmental regulation of intercellular protein trafficking through plasmodesmata in tobacco leaf epidermis. Plant Physiol. 118:373-385. 2. Woo, Y.-M., Itaya, A., Owens, R.A., Tang, L., Hammond, R.W., Chou, H.-C., Lai, M.M.C., and Ding, B. (1999). Characterization of nuclear import of potato spindle tuber viroid RNA in permeabilized protoplasts. Plant J. 17:627-635. 3. Itaya, A., Liang, G., Nelson, R.S., and Ding, B. (2000). Nonspecific intercellular protein trafficking probed by green fluorescent protein. Protoplasma 213:165-175. 4. Zhu, Y., Green, L., Woo, Y.-M., Owens, R., and Ding, B. (2001). Cellular basis of Potato spindle tuber viroid systemic movement. Virology 279:69-77. 5. Itaya, A., Folimonov, A., Matsuda, Y., Nelson, R., and Ding, B. (2001). Potato spindle tuber viroid as inducer of RNA silencing in infected tomato. Molec. Plant-Microbe Interact. 14:1332-1334. 6. Owens, R.A., Blackburn, M. and Ding, B. (2001). Possible involvement of phloem protein 2 in long distance viroid movement. Molec. Plant-Microbe Interact. 14:905-909.

PHS 398/2590 (Rev. 05/01) Page 5 Biographical Sketch Format Page Principal Investigator/Program Director (Last, First, Middle):

7. Qi, Y. and Ding, B. (2002) Replication of Potato spindle tuber viroid in cultured cells of tobacco and Nicotiana benthamiana: The role of specific nucleotides in determining replication levels for host adaptation. Virology 302:445-456. 8. Itaya, A., Ma, F., Qi, Y., Matsuda, Y., Zhu, Y., Liang, G., and Ding, B. (2002). Plasmodesma-mediated selective protein traffic between “symplasmically-isolated” cells probed by a viral movement protein. Plant Cell 14: 2071-2083. 9. Zhu, Y., Qi, Y., Xun, Y., Owens, R., and Ding, B. (2002) Movement of Potato spindle tuber viroid reveals regulatory points of phloem-mediated RNA traffic. Plant Physiol. 130:138-146. 10. Itaya, A., Matsuda, Y., Gonzales, R. A., Nelson, R.S., and Ding, B. (2002) Potato spindle tuber viroid strains of different pathogenicity induces and suppresses expression of common and unique genes in infected tomato. Molec. Plant-Microbe Interact. 15:990-999. 11. Matsuda, Y., Liang, G., Zhu, Y., Ma, F., Nelson, R.S., and Ding, B. (2002). The Commelina yellow mottle virus promoter drives companion cell-specific gene expression in multiple organs of transgenic tobacco. Protoplasma 220:51-58. 12. Qi, Y. and Ding, B. (2003). Differential subnuclear localization of RNA strands of opposite polarity derived from an autonomously-replicating viroid. Plant Cell 15:2566-2577. 13. Qi, Y. and Ding, B. (2003). Inhibition of cell growth and shoot development by a specific nucleotide sequence in a noncoding viroid RNA. Plant Cell 15:1360-1374. 14. Qi, Y., Pélissier, T., Itaya, A., Hunt, E., Wassenegger, M., and Ding, B. (2004). Direct role of a viroid RNA motif in mediating directional RNA trafficking across a specific cellular boundary. Plant Cell 16:1741-1752. 15. Qi, Y., Zhong, X., Itaya, A., and Ding, B. (2004). Dissecting RNA silencing in protoplasts uncovers novel effects of viral suppressors on the silencing pathway at the cellular level. Nucleic Acids Research 32 (22): e179. 16. Cao, X., Zhou, P., Zhang, X., Zhu, S., Zhong, X., Xiao, Q., Ding, B., Li, Y. (2005). Identification of an RNA silencing suppressor from a plant double-stranded RNA virus. J. Virol. 79:13018-13027. (Selected by Editor for Spotlight) 17. Zhong, X., Leontis, N. B., Qian, S., Itaya, A., Boris-Lawrie, K., and Ding, B. (2006). Tertiary structural and functional analyses of a viroid RNA motif by isostericity matrix and mutagenesis reveal its essential role in replication. J. Virol. 80:8566-8581. 18. Itaya, A., Zhong, X., Bundschuh, R., Qi, Y., Wang, Y., Takeda, R., Harris, A.R., Molina, C., Nelson, R.S., and Ding, B. (2007). A structured viroid RNA is substrate for dicer-like cleavage to produce biologically active small RNAs but is resistant to RISC-mediated degradation. J. Virol. 81:2980-2994 (Highlighted by Faculty 1000 Biology as “Must Read” on April 16, 2007) 19. Wang, Y., Zhong, X., Itaya, A., and Ding, B. (2007). Evidence for the in vivo existence of loop E motif of potato spindle tuber viroid. J. Virol. 81:2074-2077. 20. Zhong, X., Tao, X., Stombaugh, J., Leontis, N., and Ding, B. (2007). Tertiary structure and function of an RNA motif required for plant vascular entry to initiate systemic trafficking. EMBO J. 26:3836-3846. 21. Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y. (2007) The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc. Natl. Acad. Sci. USA 104:19547-19552. 22. Itaya, A., Bundschuh, R., Archual, A., Joung, J.-G., Fei, Z., Dai, X., Zhao, P., Tang, Y., Nelson, R.S. and Ding, B. (2007). Small RNAs in tomato fruit and leaf development. Biochim Biophy Acta 1779:99-107. 23. Zhong, X., Archual, A.J., Amin, A.A., and Ding, B. (2008) A genomic map of viroid RNA motifs critical for replication and systemic trafficking. Plant Cell 20:35-47. (Highlighted as Editor’s Choice in the February 8, 2008 issue of Science.) C. Research Support Ongoing Research Support SF IBN-0238412 Ding (PI) 8/1/2006-7/31/2009 An integrative approach to elucidate RNA replication and systemic trafficking This study investigates the RNA structural motifs and cellular factors critical for replication as well as RNA tructural motifs essential for intercellular trafficking, using potato spindle tuber viroid as the model.

PHS 398/2590 (Rev. 05/01) Page Continuation Format Page Principal Investigator/Program Director (Last, First, Middle):

Completed Research Support USDA NRICGP 2004-35304-15005 Ding (PI) 9/1/2004-8/31/2008 Biochemical and genetic analyses of plant microRNA biogenesis This project investigates how plant microRNAs are produced. NSF IBN-0238412 Ding (PI) 3/15/2003-3/14/2006 Mechanisms of phloem-mediated RNA traffic This study investigates mechanisms that control phloem-mediated long-distance RNA traffic, using potato spindle tuber viroid as the model. NSF IOB-0515745 Ding (PI) 3/1/2005-2/28/2007 Novel biogenesis and function of small RNAs derived from a plant pathogen This project studies the biogenesis and function of viroid-derived small RNAs. USDA NRICGP 2002-35304-12272 Ding (PI) 8/15/2002-8/14/2004 Cellular and genetic analysis of intercellular protein traffic The goal of this study was to investigate cellular boundaries that regulate intercellular protein trafficking and evelop genetic screening systems of Arabidopsis mutants defective in protein trafficking. The Samuel Roberts Noble Foundation Ding (PI) Nelson (CoPI) 1/1/2000-11/1/2003 Plant development and viral movement This project examined how plant development affects viral infection patterns. The Ohio Plant Biotechnology Consortium Ding (PI) 7/1/2004-6/30/2005 Development of novel microRNAs for gene function studies and for viral resistance This projects tests the idea of engineering vectors to express designer microRNAs for gene regulation and anti-viral infection. USDA NRICGP 2001-35304-09928 Ding (PI) 12/1/2000-11/30/2002 Intercellular protein trafficking and leaf development This project investigated the role of leaf developmental stages in controlling intercellular protein trafficking patterns.

PHS 398/2590 (Rev. 05/01) Page Continuation Format Page

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Doseff, Andrea I Associate Professor eRA COMMONS USER NAME DOSEFF1 EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Buenos Aires, Argentina B.S. 1987 Biology Center of Plant Ecophysiology Master 1988 Biochemistry Cold Spring Harbor Laboratory Ph.D. 1996 Genetics Cold Spring Harbor Laboratory Post-doc 1996-1998 Apoptosis

A. Positions and Honors Positions 1989 - 1990 Research Associate, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York; Dr. Venkatesan Sundaresan, Associate Professor. 1996 - 1998 Post-Doctoral Research Fellow, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York; Dr. Yuri Lazebnik, Assistant Professor. 1998 - 2002 Research Scientist, Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio; Dr. Mark Wewers, Professor. 2002 - 2008 Assistant Professor, Department of Molecular Genetics, College of Biological Sciences. Department of Internal Medicine, Division of Pulmonary and Critical Care, College of Medicine and Public Health. Davis Heart and Lung Research Institute, OSU 2008 - Associate Professor, Department of Molecular Genetics, College of Biological Sciences. Department of Internal Medicine, Division of Pulmonary and Critical Care, College of Medicine and Public Health. Davis Heart and Lung Research Institute, OSU Other Experience and Professional Memberships 2001 - 2004 Member of the Allergy and Immunology Assembly Program Committee. American Thoracic Society (ATS). 2002 - 2004 Member of the Allergy and Immunology Assembly Planning Committee. ATS. 1996 - Present Cell Death Society 1999 - Present American Lung Association. 2002 - Present Ohio State University-Comprehensive Cancer Center 2002 - Present Heart and Lung Research Institute 2005 - Present Mathematical Biosciences Institute, Scientific Advisory Committee 2007 - Present Member Public Health Preparedness for Infectious Diseases 2007 - Present Member Center for Advanced Functional Foods and Research Entrepreneurship 2007 - Present Chair Basic/Clinical Scientist Partnership, College Medicine, OSU 2008 - Present American Society for Biochemistry and Molecular Biology 2003 - Present Reviewer: J. Biological Chemistry, Blood, Biochemical Pharmacology, Cell Death and Differentiation, Journal of Leukocyte Biology, Apoptosis, American Journal of Physiology, Stem Cell and Development, PlosOne Genetics, American Journal of Respiratory and Critical Care Medicine. Editor: Frontiers in BioSciences (FBS) 2007 - Present American Heart Association, grant reviewer 2007 - Present Ad-hoc National Institute of Health. Lung Cellular, and Immunobiology Study Section 2007 - Present National Science Foundation: Molecular Cellular Biology-Panel II Cellular Signaling 2008 - Co-Chair Basic Cell and Molecular Biology. American Heart Association Honors 1988 – 1989 Argentine National Research Council (CONICET) Graduate Fellow 1996 – 1998 NIH Training Grant Postdoctoral Fellow

1999 – 2001 American Lung Association, Research Grant 2007 Nominated for OSU-Distinguished Undergraduate Research Mentor of the Year Patents 1. Inhibition of monocyte survival, differentiation or proliferation. Inventors: Andrea I. Doseff and Erich Grotewold. U.S.Patent # PCT/US06/020905 60/684,655. Issue 26/05/06 2. Invention Disclosure. The small Heat shock protein 27 (Hsp27) is a marker for cellular life span. Inventor: Andrea I. Doseff. 9/12/06 B. Selected peer-reviewed publications (1998-present) 1. Kelly, T., Graham, M., Doseff, A.I., Pommerantz, R.W., Lau, S.M., Ostrowski, M.C., Franke, T.M., and Marsh, C. B. (1999). Macrophage colony-stimulating factor promotes cell survival through Akt/protein kinase B. J. Biol. Chem. 274: 26393-26398. 2. Kirsch, D.G., Doseff, A.I., Chau, B.N., Lim, D., Souza-Pinto, N.C., Hansford, R., Kastan, M.B., Lazebnik, Y.A., and Hardwick, J.M. (1999). Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c. J. Biol. Chem. 274: 21155-21161. 3. Fahy, R.J.*, Doseff, A.I.*, and Wewers, M.D. (1999). Spontaneous human monocyte apoptosis utilizes a caspase-3 dependent pathway which is blocked by endotoxin and is independent of caspase-1. J. Immunol. 163: 1755-1762. 4. Goyal, A., Wang, Y., Graham, M.M., Doseff, A.I., Bhatt, N.Y., and Marsh, C.B. (2002). Monocyte survival factors induce Akt activation and suppress caspase-3. Am. J. Resp. Cell and Mol. Biol. 26: 224-230. 5. Coulter, K.R., Sweeney, P., Doseff, A.I., Wang, Y., Marsh, C.B, Wewers, M.D., Knoell, D.L. (2002). Opposing effect by cytokines on Fas-mediated apoptosis in A549 lung epithelial cells. Am. J. Resp. Cell and Mol. Biol. 26: 58-66. 6. Rovin, B.H., Wilmer, W.A., Lu, L., Doseff, A.I., Dixon, C., Kotur, M., and Hilbelink, T. (2002). The 12,14 cyclopentenone prostaglandin 15-deoxy-Δ -prostaglandin J2 regulates mesangial cell proliferation and death. Kidney Intern. 61:1293-302. 7. Zeigler, M.M., Doseff, A.I., Galloway, M.F., Opalek,J.M., Nowicki, P.T., Zweier, J.L., Sen, C.K and Marsh, C.B. (2003). The presentation of nitric oxide regulates monocyte life and death through effects on caspase-9 and caspase-3 activation. J. Biol. Chem. 278: 12894-129 8. Doseff, A.I., Baker Jr., J.H., Bourgeois, T.A., and Wewers, M.D. (2003). IL-4-induced apoptosis entails caspase activation and dephosphorylation of ERK. Am. J. Resp. Cell and Mol. Biol. 29: 367-374 9. Elssner, A., Doseff, A.I., Duncan, M., Kotur, M., and Wewers, M.D. (2004). Interleukin-16 is constitutively present in peripheral blood monocytes and spontaneoulsy released during apoptosis. J. Immunol. 172: 7721-7725. 10. Wei G, Guo, J, Doseff, AI, Kusewitt, DF, Man, AK, Oshima, RG, and Ostrowski, MC. (2004). Activated Ets2 is required for persistent inflammatory responses in the motheaten viable model. J. Immunol. 173:1374-1379. 11. Bao, S., Sweeney, P., Wang, Y., Chaudhuri, A, Doseff, A.I. Marsh, C.B., and Knoell, D.L. (2005). Keratinocyte Growth Factor induces Akt kinase and prevents Fas-mediated apoptosis in A549 lung epithelial cells. A.J.P. Lung Cell. Mol. Physiol. 288 (1): L36-42. 12. Doseff, A.I. (2004). Apoptosis: the sculptor of development. Stem Cells and Development.13: 473-483. 13. Voss, O.H., Kim, S., Wewers, M.D. and Doseff, A.I. (2005). Caspase-3 is a phosphoprotein and associates with PKCδ during spontaneous monocyte apoptosis. J. Biol. Chem. 280: 17371-17379. 14. Vargo, M.A., Voss, O.H., Poustka, F., Cardounel, A.J., Grotewold, E., and Doseff, A.I. (2006). Apigenin- induced-apoptosis is mediated by the activation of PKCδ and caspases in leukemia cells. Biochem. Pharmacol. 72: 681-692 15. Voss, O.H., Batra, S., Kolattukudy, S.J., Smith, J.B., and Doseff, A.I. (2007). Heat shock protein 27 regulates monocyte life span by inhibiting caspase-3 activation. J. Biol. Chem. 282: 25088-25099. 16. Nicholas, C., Batra, S., Vargo, M.A., Voss, O.H., Gavrilin, M.A., Wewers, M.D., Guttridge, D., Grotewold, E., and Doseff, A.I. (2007). Apigenin, a plant flavonoid, inhibits inflammatory cytokines and induces apoptosis in LPS treated human monocyte. J. Immunol. 179:7121-7127. 17. Gonzalez-Mejia, M.E. and Doseff, A.I. (2008). Regulation of monocytes and macrophages cell fate. Frontiers in Biosciences (accepted).

18. Malavez, Y., Gonzalez-Mejia, M.E., and Doseff, A.I. (2008). PKCδ. Atlas Genet Cytogenet Oncol Haematol. URL: http://AtlasGeneticsOncology.org/Genes/ 19. Wood, K.L., Twigg III, H.L., Doseff, A.I. (2008). Dysregulation of CD8+ lymphocyte apoptosis, chronic disease, and immune regulation. Frontiers in Biosciences (accepted). 20. Wood, K.L., Voss, O,H,. Huang, Q., Parihar, A., Metha, N., Batra, S., and Doseff, A.I. (2008). The small heat shock protein 27 is a key regulator of CD8+ CD57+ lymphocyte survival. (Submitted). 21. Doolittle, J., Aguda, B., and Doseff, A.I. Integrative modeling of PKCδ-mediated activation of caspase-3 during apoptosis. (BMC Systems Biology, In preparation). * Indicates shared first authorship C. Research Support Ongoing Support NHI-HLBI (RO1) Doseff (PI) 12/01/05-11/30/10 Title: Molecular mechanisms of apoptosis in monocytes NSF (RIG/CAA) Doseff (PI) 1/01/06-12/31/08 Title: Regulation of apoptosis by the interaction of casp-3 with PKC and small heat shock protein National Science Foundation (REU) Doseff (co-PI) 4/01/06-3/31/08 Research Undergraduate Education Grant PI: Drs. A. Simcox and V. Gopalan NIH-NHLI Doseff (PI) 6/01/07-12/31/10 Title: Molecular mechanisms of apoptosis in monocytes, Supplemental for PhD student American Heat Association (Predoctoral) Doseff (Mentor) 7/01/06-6/30/08 Title: Regulation of human monocyte apoptosis by caspase-3 NSF-MCB Doseff (PI) 5/31/07-4/3/08 Title: Regulation of apoptosis by the interaction of casp-3 with PKC and small heat shock protein NIH-NHLI (RO1) Doseff (Co-PI) 4/01/06-3/31/10 Title: Methylarginines and vascular injury PI: Dr. A.J. Cardounel Bremen Award Doseff (co-PI) 9/01/06-8/31/08 Title: Heat shock protein 27: alloimmunity and apoptosis PI: Dr K. Wood Mayers Summer Research Internship Doseff (sponsor) 6/01/08-9/15/08 Undergraduate Fellowship Student: Eric Murnan Title: Role of heat shock proteins in cell death Completed Research Support Mayers Summer Research Internship Doseff (sponsor) 6/01/07-9/15/07 Undergraduate Fellowship Student: Janet Doolittle Mechanisms of the regulation of apoptosis: mathematical and system biology Glenn-Stokes Summer internship Doseff (sponsor) 6/01/07-9/15/07 Undergraduate Fellowship Student: Mitila Arasu American Health Assistant Foundation Doseff (PI) 5/01/04-7/01/05 Regulatory mechanisms of human monocyte apoptosis by IL-4 American Cancer Society Doseff (PI) 10/01/04-9/30/05 Molecular mechanisms of caspase-3 regulation by hsp27 in monocytes American Lung Association Doseff (PI) 7/01/01-8/01/03 Title: Role of caspase-3 regulation in human monocyte apoptosis.

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Fisk, Harold A. Assistant Professor eRA COMMONS USER NAME FISK13 EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Colorado, Boulder B.A. 1989 Mol. Biol./Chemistry University of California, San Diego Ph.D. 1998 Cell Biology A. Professional Preparation University of Colorado, Boulder Mol. Biol./Chemistry B.A. 1989 University of California, San Diego Cell Biology Ph.D. 1998 University of Colorado, Boulder Cell Biology 1998-2004 A. Positions and Honors Positions and Employment 1989-1992 Professional Research Assistant, MCD Biology, University of Colorado, Boulder 1992-1998 Graduate Research Assistant and Graduate Teaching Assistant, Department of Biology University of California, San Diego 1998 Postdoctoral Fellow, Department of Biology, University of California, San Diego 1998-2004 Postdoctoral Fellow, MCD Biology, University of Colorado 2004-present Assistant Professor, Department of Molecular Genetics, (80% appointment) and Human Cancer Genetics Program (20% appointment) The Ohio State University Honors and Awards 1989 Golden Key National Honor Society, University of Colorado, Boulder 1994 Teaching Excellence Award, University of California, San Diego 1999 Research Fellowship Award, Jane Coffin Childs Memorial Fund for Medical Research 2002 Special Fellow Award, Leukemia and Lymphoma Society 2004 Ohio State University Comprehensive Cancer Center Institutional ACS Fund Seed Grant Award 2004 American Cancer Society, Ohio Division Seed Grant Award 2006 Selected as OSU candidate in limited submission competition, Mary Kay Ash Foundation Teaching Experience 1993 Graduate Teaching Assistant, Biochemical Lab Techniques, University of California, San Diego 1994 Graduate Teaching Assistant, Cell Biology Laboratory, University of California, San Diego 1995 Graduate Teaching Assistant, Molecular Biology (lecture), University of California, San Diego 2003 Lecturer, MCDB3150, Biology of the Cancer Cell (Undergraduate), University of Colorado (12 lecture module) 2004-present Instructor, IBGP701.03, Genes and Genomes (Graduate), OSU (4 lecture module) 2006-present Instructor, MG602, Eukaryotic Cell and Developmental Biology Laboratory (Undergraduate) OSU (10 5-hour lab sessions, Dr. Harold Fisk course director) 2007-present Instructor, MG705, Advances in Cell Biology (Graduate), OSU (9 lecture module) Selected Professional Affiliations 1995-present Member, American Association for Cell Biology 2004-present Member, American Association for the Advancement of Science 2004-present Member, National Center for Science Education 2004-present Member, MCDB interdisciplinary graduate program 2004-present Member, OSBP interdisciplinary graduate program 2005-present Member, OSU Comprehensive Cancer Center 2005 Co-organizer, OSUCCC MBCG Retreat, November 10-11 2005 2007 Co-organizer, OSUCCC MBCG Retreat, November 10-11 2007 Recent Professional Activities 2004-present Manuscript Review EMBO Reports, Journal of Cell Biology, Current Biology, Molecular and Cellular Biology, Cell Cycle, FEBS Letters, FASEB J, Molecular Biology of the Cell, Cancer Biology & Therapy.

PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page 1 Continuation Format Page 2005 Ad hoc Grant Reviewer, Cancer Research UK 2006, 2008 Grant Review Panel Member, Texas Advanced Research Program 2007 Grant Review Panel Member, OSUCCC Institutional ACS Funds Seed Grant Program 2007-present Honors Advisor, Molecular Genetics Major Program The Ohio State University 2008-present Grant Review for Department of Molecular Genetics Internal Grant Review Committee B. Publications: Peer-reviewed publications (11 of 11 in chronological order). Kano-Sueoka, T., King, D. M., Fisk, H. A. and Klug, S. J. (1990). Binding of Epidermal Growth Factor to its Receptor is Affected by Membrane Phospholipid Environment. J Cell Physiol 145: 543-548. Fisk, H. A. and Kano-Sueoka, T. (1992). Effect of Membrane Phosphatidylethanolamine- Deficiency/Phosphatidylcholine-Excess on the Metabolism of Phosphatidylcholine and Phosphatidylethanolamine. J Cell Physiol 153: 589-595. Zha, H., Fisk, H. A., Yaffe, M. P., Mahajan, N., Herman, B. and Reed, J. C. (1996). Structure-Function Comparisons of the Proapoptotic Protein Bax in Yeast and Mammalian Cells. Mol Cell Biol 16: 6494-6508. Fisk, H. A. and Yaffe, M. P. (1997). Mutational Analysis of Mdm1p Function in Nuclear and Mitochondrial Inheritance. J Cell Biol 138: 485-494. Fisk, H. A. and Yaffe, M. P. (1999). A Role for Ubiquitination in Mitochondrial Inheritance in Saccharomyces Cerevisiae. J Cell Biol 145: 1199-1208. Fisk, H. A. and Winey, M. (2001). The Mouse Mps1p-Like Kinase Regulates Centrosome Duplication. Cell 106: 95-104. Fisk, H. A., Mattison, C. P. and Winey M. (2003). The Human Mps1 Protein Kinase is Required for Centrosome Duplication and Normal Mitotic Progression. Proc. Nat. Acad. Sci. 100: 14875-14880. Fisk, H. A., Mattison, C. P., and Winey, M. (2004). A Field Guide to the Mps1 Protein Kinases. Cell Cycle 3(4) 439-442. Fisk, H. A. and Winey, M. (2004) Mps1 flies into new areas. Current Biology 14(24):R1058-60. Kasbek, C. Yang, C.-H., Chapman, H. M., Mohd Yusof, A., Winey, M., and Fisk, H. A. (2007). Preventing the degradation of Mps1 at centrosomes is sufficient to cause centrosome re-duplication in human cells. Mol. Biol. Cell. 18(11): 4457-69. Kasbek, C., Yang., C.-H., and Fisk, H.A. (2008). Mps1 as a link between centrosomes and genomic instability. Env. Mol. Mut. in press. Reviews and Book Chapters (5 of 5 in chronological order) Fisk, H. A., Mattison, C. P., and Winey, M. (2002). Centrosomes and Tumor Suppressors. Curr Op Cell Biol 14: 700-705. Fisk, H. A., Mattison, C. P., and Winey, M. (2004). A Field Guide to the Mps1 Protein Kinases. Cell Cycle 3(4) 439-442. Fisk, H. A. and Winey, M. (2004) Mps1 flies into new areas. Current Biology 14(24):R1058-60. Yang, C.-H., Kasbek, C., and Fisk, H. A. (2008) The use of infrared fluorescent dyes in quantitative immunoblotting. in Protein Protocols Handbook, Third Edition. Humana Press, London (In Press). Kasbek, C., Yang, C.-H., and Fisk, H. A. (2008) The use of infrared fluorescent dyes in immunofluorescence microscopy. in Protein Protocols Handbook, Third Edition. Humana Press, London (In Press). C. Research Support. Ongoing Research Support GM077311-01 Fisk (PI) 4/01/2008-3/31/2013 The Ohio Cancer Research Associates Regulation of the Mps1 protein kinase and centrosome duplication 60012435 Fisk (PI) 1/01/2006-12/31/2006 The Ohio Cancer Research Associates Regulation of the centrosomal degradation of the Mps1 protein kinase Completed Research Support GRT00003611 Fisk (PI) 1/01/2006-12/31/2006 The American Cancer Society, Ohio Division Inc. The role of the Mps1 Protein Kinase and Centrosomes in Leukemia 60003037 Fisk (PI) 10/01/2005-9/31-2006 The Ohio State University Comprehensive Cancer Center Institutional ACS Fund MCF7 Cells as a Model for Mps1 and Centrosome Function Seed grant award for the development of a cell-based in vitro centrosome duplication assay

PHS 398/2590 (Rev. 09/04, Reissued 4/2006) Page 7 Continuation Format Page

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Grotewold, Erich Professor of Plant Cellular & Molecular Biology

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Buenos Aires Ph.D. 1988 Chemistry University of Buenos Aires B.Sc. 1985 Chemistry

A. Positions and Honors. Positions and Employment 1989-1993 Post-Doctoral Research Fellow, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 1993-1995 Staff Associate, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 1995-1997 Assistant Investigator, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 1997-2001 Assistant Professor, Department of Plant Cellular and Molecular Biology and Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, OH. 2001-2006 Associate Professor, Department of Plant Cellular and Molecular Biology and Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, OH 2006- Professor, Department of Plant Cellular and Molecular Biology, The Ohio State University, Columbus, OH 2007 - 2008 Visiting Professor, Mathematical Bioscience Institute, The Ohio State University, Columbus, OH 2008 - Associate Director, Arabidopsis Biological Resource Center Other Professional Experiences 1997- Member, Genetics Society of America 1997- Member, American Society of Plant Biology 1999 - Panel Member, Biochemistry of Gene Expression Program at the NSF 2002, 2004 Panel Member, Interagency Activity in Metabolic Engineering at the NSF 2000- Member, American Society of Cell Biology 2002 - Member, Phytochemical Society of North America 2004 - Member, American Society for Biochemistry and Molecular Biology 2004 Panel Member, Plant Biochemistry at the US Dept. of Agriculture 2005 Director, Summer Practical Workshop in Functional Genomics, OSU 2005 – 2007 Co-Founder, Co-Chair (2005) and Chair (2007), Gordon Research Conference in Plant Metabolic Engineering 2006 - Member, Editorial Board Molecular Biotechnology 2006 - 2008 Member, Editorial Board BMC Plant Biology 2008 - Associate Editor, BMC Plant Biology 2008 Editor, Special Issue Current Opinions in Biotechnology Honors 2006 Dean’s Award for Excellence in Undergraduate Research Mentoring 1995 Demerec-Kaufmann-Hollaender Fellow in Developmental Genetics B. Selected publications (out of 73 total). Poustka, F., Irani, N.G., Feller, A., Lu, Y., Pourcel, L., Frame, K., Grotewold, E. (2007) A trafficking pathway for anthocyanins overlaps with the endoplasmic reticulum-to-vacuole protein sorting route in Arabidopsis and contributes to the formation of vacuolar inclusions. Plant Physiol. 145: 1323-1335 Hernandez, J.M., Feller, A., Morohashi, K., Frame, K. and Grotewold, E. (2007) The bHLH domain of maize R links transcriptional regulation and histone modifications by recruitment of an EMSY-related factor. Proc. Natl. Acad. Sci. USA. 104: 17222-17227. 1

Morohashi, K., Zhao, M., Yang, M., Read, B., Lloyd, A., Lamb, R., and Grotewold, E. (2007) Participation of the Arabidopsis bHLH factor GL3 in trichome initiation regulatory events. Plant Physiol. 145: 736-746. Feller, A., Hernandez, J.M., and Grotewold, E. (2006) An ACT domain participates in the dimerization of several plant bHLH transcription factors. J. Biol. Chem. 281: 28964 – 28974. Grotewold, E. (2006) The genetics and biochemistry of flower pigments. Ann. Rev. Plant Biol. 57: 761-780. Molina, C., and Grotewold, E. (2005) Genome-wide analysis of Arabidopsis core promoters. BMC Genomics 6: 25. Grotewold, E. (2005) Plant metabolic diversity: A regulatory perspective. Trends Plant Sci. 10: 57-62. Hernandez, J., Heine, G., Irani, N.G., Feller, A., Kim, M.-G., Matulnik, T., Chandler, V.L., and Grotewold, E. (2004) Different mechanisms participate in the R-dependent activity of the R2R3 MYB transcription factor C1. J. Biol. Chem. 279: 48205-48213. Heine, G.F., Hernandez, J.M., and Grotewold, E. (2004) Two cysteines in plant R2R3 MYB domains participate in REDOX-dependent DNA binding. J. Biol. Chem. 279: 37878-37885. Lin, Y., Irani, N.G., and Grotewold, E. (2003) Sub-cellular trafficking of phytochemicals explored using auto- fluorescent compounds in maize cells. BMC Plant Biology 3: 10. Davuluri, R.V., Sun, H., Palaniswamy, S.K., Matthews, N., Molina, C., Kurtz, M., and Grotewold, E. (2003) AGRIS: Arabidopsis Gene Regulatory Information Server, an information resource of Arabidopsis cis- regulatory elements and transcription factors. BMC Bioinformatics 4: 25 – 35. Dias, A.P., Braun, E.L., McMullen, M.D., and Grotewold, E. (2003) Recently duplicated maize R2R3 Myb genes provide evidence for distinct mechanisms of evolutionary divergence after duplication. Plant Physiol. 131: 610-620. Pooma, W., Gersos, C., and Grotewold, E. (2002) Transposon insertions in the promoter of the Zea mays a1 gene differentially affect transcription by the Myb factors P and C1. Genetics 161: 793-801. Dong, X., Braun, E.L., and Grotewold, E. (2001) Functional conservation of plant secondary metabolic enzymes revealed by complementation of Arabidopsis flavonoid mutants with maize genes. Plant Physiol. 127: 46-57. Grotewold, E., Sainz, M.B., Tagliani, L., Hernandez, J.M., Bowen, B., and Chandler, V.L. (2000) Identification of the residues in the Myb domain of C1 that provide the specificity of the interaction with the bHLH cofactor R. Proc. Natl. Acad. Sci. USA 97: 13579-13584. Braun, E.L., and Grotewold, E. (1999) Newly discovered plant c-myb-like genes rewrite the evolution of the plant myb gene family. Plant Physiol. 121: 21-24. Rabinowicz, P. D., E. L. Braun., A. D. Wolfe, B. Bowen, and E. Grotewold. (1999) Maize R2R3 Myb genes: Sequence analysis reveals amplification in higher plants. Genetics 153: 427-444. Grotewold, E., Chamberlain, M., St. Claire, G., Swenson, J., Siame, B.A., Butler, L.G., Snook, M. and Bowen, B. (1998) Engineering secondary metabolism in maize cells by ectopic expression of transcription factors. Plant Cell 10: 721-740. Williams, C.E. and Grotewold, E. (1997) Differences between plant and animal Myb domains are fundamental for DNA-binding activity and chimeric Myb domains have novel DNA-binding specificities. J. Biol. Chem. 272: 563-571. Grotewold, E., Drummond, B., Bowen, B. and Peterson, T. (1994). The Myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset. Cell 76: 543-553. Grotewold, E., Athma, P. and Peterson, T. (1991). Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of Myb-like transcription factors. Proc. Natl. Acad. Sci 88: 4587-4591. BOOKS EDITED Grotewold, E. (Editor) The Science of Flavonoids. (2006) Springer, New York, NY. Grotewold, E. (Editor) Plant Functional Genomics: Methods & Protocols. (2003) Humana Press, Totowa, NJ. PATENTS AND INVENTIONS Transgenic plants with altered levels of phenolic compounds. Pat. No. 7,154,023. Transgenic turfgrasses which signal exposure to chemicals and stress conditions. Pat. No. 6,709,867 Inhibition of monocyte survival, differentiation, or proliferation. Application No. PCT/US06/020905

2 HAMEL, Patrice

NAME Patrice Hamel POSITION TITLE Assistant Professor

EDUCATION/TRAINING DEGREE (if INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY applicable) University of Paris XI, Orsay, France M.S. (eq.) 1993 1999 Cellular and Molecular Genetics University of Paris XI, Orsay, France Ph.D. post- 1999-2005 Cellular and Molecular Genetics University of California, Los Angeles doctoral Organellar Biochemistry

A. PROFESSIONAL POSITIONS AND HONORS

Previous positions From November 1999-June 2005: Post-graduate Researcher Department of Chemistry & Biochemistry, University of California, Los Angeles

July 2005-October 2005: Assistant Project Scientist, Department of Chemistry & Biochemistry, University of California, Los Angeles

Present positions From November 2005: Assistant Professor, Department of Plant and Cellular Molecular Biology and Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus.

Other professional activities -Reviewer for EMBO Journal, Biochimica and Biophysica Acta, Molecular and General Genetics, Molecular Microbiology, FEBS letters, Proceedings of the National Academy of Sciences USA, Plant Journal, Journal of Biological Chemistry, Eukaryotic Cell, Biochemistry and Journal of Bacteriology. - Ad hoc reviewer for USDA, NSF

Memberships: Associate Investigator, the Dorothy M. Davis Heart and Lung Research Institute, the Ohio State UniversityMember of the Institute of Mitochondrial Biology, The Ohio State University Genetics Society of America

Honors and Awards American Heart Association Post-doctoral Fellowship, 2001-2003 Paul Boyer Award for Excellence in Post-doctoral Research, 2003 American Heart Association Post-doctoral Fellowship, 2003 (declined, duplicate funding) Muscular Dystrophy Association Research Development Award 2003-2006

B. RESEARCH PUBLICATIONS (in chronological order)

(1) Bonnefoy, N., Chalvet, F., Hamel P., Slonimski, P.P. & Dujardin G., (1994) OXA1, a Saccharomyces cerevisiae nuclear gene whose sequence is conserved from prokaryotes to eukaryotes controls cytochrome oxidase biogenesis. J. Mol. Biol., 239, 201-212. - (2) Hamel, P., Sakamoto, W., Wintz, H. & Dujardin, G., (1997) Functional complementation of an oxa1 yeast mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes. Plant J., 12, 1319-1327. (3) Hamel, P., Lemaire, C., Bonnefoy, N., Brivet-Chevillotte, P. & Dujardin G., (1998) Mutations in the membrane anchor of yeast cytochrome c1 compensate for the absence of Oxa1p and generate carbonate- extractable forms of cytochrome c1. Genetics, 150, 601-611. (4) Cardazzo, B., Hamel, P., Wintz, H., Sakamoto, W. & Dujardin, G., (1998). Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity. Gene 221, 117-125. (5) Arai, M., Hamel, P., Kanaya, E., Inaka, K., Miki, K., Kikuchi, M. & Kuwajima, K. (2000) Effect of an alternative disulfide bond on the structure, stability and folding of human lysozyme. Biochemistry 39, 3472- 3479. (6) Hamel, P., Olive, J., Pierre, Y., Wollman, F.-A. & de Vitry, C. (2000) A new subunit of cytochrome b6 f complex undergoes reversible phosphorylation upon state transition. J. Biol. Chem. 275, 17072-17079. (7) Lemaire, C., Hamel, P., Velours, J. and Dujardin, G. (2000) Absence of the mitochondrial AAA protease Yme1p restores ATPase F0 subunit accumulation in an oxa1 deletion mutant of Saccharomyces cerevisiae. J. Biol. Chem. 275, 23471-23475. (8) Nakamoto, S., Hamel, P. & Merchant, S., (2000) Assembly of chloroplast cytochromes b and c. Biochimie 82, 603-614. (9) Saint-Georges, Y., Hamel, P., Lemaire, C., and Dujardin, G. (2001) Role of positively-charged transmembrane segments in the insertion and assembly of mitochondrial inner-membrane proteins. PNAS 98, 13814-13819. (10) Hamel, P., Dreyfuss, B., Xie, Z., Gabilly, S. and Merchant S. (2003) Essential histidine and tryptophan residues in CcsA, a system II polytopic cytochrome c biogenesis protein. J. Biol. Chem. 278, 2593- 2603. (11) Dreyfuss, B., Hamel, P., Nakamoto, S. and Merchant S. (2003) Functional analysis of a divergent system II protein, Ccs1, involved in c-type cytochrome biogenesis. J. Biol. Chem. 278, 2604-2613. (12) Bernard, D., Gabilly, S., Dujardin, G., Merchant, S. and Hamel, P. (2003) Overlapping specificities of the cytochrome c and c1 heme lyases. J. Biol. Chem. 50, 49732-49742. (13) Hamel, P., Saint-Georges, Y., Altamura N., de Pinto, B. and Dujardin, G. (2004) Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana. Mol. Microbiol. 51, 307-317. (14) Page, M*., Hamel, P*., Gabilly, S., Zegzouti, H, Perea, J., Alonso, J., Ecker, J., Theg, S., Christensen, S., and Merchant, S. (2004) A homolog of the prokaryotic thiol-disulfide transporter CcdA is required for the assembly of the cytochrome b6f complex in Arabidopsis chloroplasts. J. Biol. Chem. 279, 32474-32482. (* equal contribution). (15) Bernard, D.B., Quevillon-Cheruel, S., Merchant S., Guiard, B. and P. Hamel (2005) Cyc2p, a membrane bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes. J. Biol. Chem. 280, 3985239859. (16) Hanikenne M., Merchant, S. and Hamel, P. (in press) Transition metal nutrition: a balance between deficiency and toxicity. Chlamydomonas in the Plant Science, Vol.2 of the Chlamydomonas Sourcebook (Ed. D. Stern, Publisher: Elsevier Inc.). (17) Remacle C., Barbieri, R., Cardol P., and Hamel, P. Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process Molecular Genetics and Genomics 280, 93-110. (18) Hamel P., Corvest V., Giege P and Bonnard G.. Biochemical requirements for the maturation of mitochondrial c-type cytochromes Biochimica Biophysica Acta-Molecular Cell Research (in press, DOI:10.1016/j.bbamcr.2008.06.017). (19) Claire, R., Cline, S., Boutafalla, L., Gabilly, Larosa, V., Barbieri, R. M. and Hamel, P The ARG9 gene encodes the plastid resident N-acetyl ornitine aminotransferase in the green alga Chlamydomonas reinhardtii Eukaryotic Cell (accepted pending revisions).

C. RESEARCH SUPPORT

On-going-research support:

Unraveling the mitochondrial redox pathway in cytochrome c maturation Principal investigator: Patrice Hamel Agency: Muscular Dystrophy Association Type: Neuromuscular disease research grant MDA4247 (Years 07-09) Period: 1 Jan, 2007 - 31 Dec, 2009

This project focuses on the genetic and biochemical dissection of the intermembrane space thioredox pathway operating in the maturation of c-type cytochromes in yeast mitochondria.

Molecular genetic dissection of mitochondrial complex I assembly Principal investigator: Patrice Hamel Agency: United Mitochondrial Disease Foundation Type: Research grant Period: 17 Jul, 2007 - 16 June, 2009 This project aims to discover novel assembly factors for mitochondrial complex I using insertional mutagenesis of the nucleus in the green alga Chlamydomonas reinhardtii.

Exploration of cellular functions of organellar coiled-coil proteins in plant model systems. Do plastid and mitochondria have a cytoskeleton-like structure? Principal investigator: Iris Meier Co-principal investigator: Patrice Hamel Agency: Ohio Plant Biotechnology Consortium Type: Research grant Period: 1 Jul, 2008 – 30 June, 2010

This project aims to investigate in Arabidopis and Chlamydomonas the function of a class of novel proteins called coiled-coil proteins with mitochondrial or plastid localization.

Conservation of molecular mechanisms controlling the biogenesis of respiratory and photosyntheticcomplexes Principal investigator: Geneviève Dujardin (CNRS) Co-principal investigator: Patrice Hamel Agency: Centre National de la Recherche Scientifique (CNRS, France) Type: Travel grant Period: 1 Jan, 2008 – 31 Dec, 2010

This grant aims to support international collaboration at major american institutions for projects that are already on-going in the american host laboratory. The funds can only be used to support the visit of members from a french laboratory to the PI’s group. The co-PI has no financial responsibility over this grant.

Assembly of mitochondrial c-type cytochromes: Emergence of novel redox factors Principal investigator: Vincent Corvest Co-principal investigator: Patrice Hamel Agency: American Heart Association Type: Post-doctoral fellowship Period: 1 Jul, 2008 – 30 June, 2010

The goal of this project is to decipher the biochemical activity of Cyc2p, a novel flavoprotein involved in the assembly of c-type cytochromes in yeast mitochondria.

Regulation of H2 and CO2 Metabolism: Factors Involved in Partitioning of Photosynthetic Reductant in Green Algae Principal investigator: Maria Ghirardi (NREL) Co-principal investigator: Patrice Hamel Agency: Department of Energy Type: Research grant Period: 1 Oct, 2008 – 30 Sept, 2011

The goal of this project is to generate an insertional mutant library in Chlamydomonas and screen for strains that are attenuated for hydrogen production. The long term goal is to uncover the genes controlling this process in order to engineer algal strains that overproduce hydrogen.

Completed research support:

Functional dissection of a cytochrome c assembly machinery in mitochondria Principal investigator: Patrice Hamel Agency: Muscular Dystrophy Association Type: Neuromuscular disease research development grant MDA3618 (Years 01-03) Period: 1 Jul, 2003 - 30 Sept, 2006 (no cost extension)

This project focuses on the functional identification of novel components involved in a c-type cytochrome assembly complex in yeast mitochondria

Unravelling the mechanisms of cytochrome c biogenesis Principal investigator: Patrice Hamel Agency: American Heart Association Western Affiliates Type: Post- doctoral Fellowship 0120100Y (Years 01-02) Period: 1 Jul,2001 - 30 Jun, 2003

The goals of this project were to define the biochemistry of well-characterized c-type cytochrome assembly components in plastids of green alga and mitochondria of yeast and discover novel biogenesis factors. BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Paul K. Herman Associate Professor of Molecular Genetics eRA COMMONS USER NAME

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Manitoba, Winnipeg, Canada B.Sc. Honors 1981 Chemistry/Microbiology California Institute of Technology, Pasadena, CA Ph.D. 1991 Biology University of California, Berkeley CA 1996 Molecular Genetics

A. Positions and Honors. 1996 – 2003 Assistant Professor of Molecular Genetics, OSU 2003 – present Associate Professor of Molecular Genetics, OSU

B. Selected peer-reviewed publications (in chronological order). 1. Banta, L. M., T. A. Vida, P. K. Herman and S. D. Emr. (1990) Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. Molecular and Cellular Biology 10: 4638 - 4649. 2. Herman, P. K. and S. D. Emr. (1990) Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Molecular and Cellular Biology 10: 6742 - 6754. 3. Klionsky, D. J., P. K. Herman and S. D. Emr. (1990) The fungal vacuole: composition, function and biogenesis. Microbiological Reviews 54: 266 - 292. 4. Vida, T. A., P. K. Herman, S. D. Emr and T. R. Graham. (1991) Compartmentalized transport, modification, and sorting of yeast vacuolar hydrolases. Biomedica Biophysica Acta 50: 413 - 420. 5. Herman, P. K., J. H. Stack, J. H. DeModena and S. D. Emr. (1991) A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole. Cell 64: 425 - 437. 6. Herman, P. K., J. H. Stack and S. D. Emr. (1991) A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein sorting. EMBO Journal. 10: 4049 - 4060. 7. Stack, J. H., P. K. Herman and S. D. Emr. (1992) A protein kinase/lipid kinase complex required for yeast vacuolar protein sorting. In Molecular Mechanisms of Membrane Traffic. Vol. H74. Eds. Morre, D. J., K. M. Howell and J. J. M. Bergeron. Springer-Verlag, Heidelberg/New York. pp.363-366. 8. Herman, P. K., J. H. Stack and S. D. Emr. (1992) An essential role for a protein and lipid kinase complex in secretory protein sorting. Trends in Cell Biology 2: 363 - 368. 9. Stack, J. H., P. K. Herman, P. V. Schu and S. D. Emr. (1993) A membrane-associated complex containing the Vps15 protein kinase and Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole. EMBO Journal 12: 2195 - 2204. 10. Stack, J. H., P. K. Herman, D. B. DeWald, E. Marcusson, J. Lin Cereghino, B. Horazdovsky and S. D. Emr. (1995) Novel protein kinase/phosphatidylinositol 3-kinase complex essential for receptor- mediated protein sorting to the vacuole in yeast. Cold Spring Harbor Symposia on Quantitative Biology 60: 157 - 170. 11. Herman, P. K. and J. Rine. (1997) Yeast spore germination: a requirement for Ras protein signaling during re-entry into the cell cycle. EMBO Journal 16: 6171 - 6181. 12. Chang, Y.-W., S. C. Howard, Y. V. Budovskaya, J. Rine and P. K. Herman. (2001) The rye mutants identify a role for Ssn/Srb proteins of the RNA pol II holoenzyme during stationary phase entry in Saccharomyces cerevisiae. Genetics 157: 17-26. 13. Howard, S. C., Y.-W. Chang, Y. V. Budovskaya and P. K. Herman. (2001) The Ras/PKA signaling pathway of Saccharomyces cerevisiae exhibits a functional interaction with the Sin4p complex of the RNA polymerase II holoenzyme. Genetics 159: 77-89. 14. Budovskaya, Y. V., H. Hama, D. B. DeWald. and P. K. Herman. (2002) The C-terminus of the Vps34p phosphoinositide 3-kinase is necessary and sufficient for interaction with the Vps15p protein kinase. Journal of Biological Chemistry 277: 287-294. 15. Howard, S. C., Y. V. Budovskaya, Y.-W. Chang and P. K. Herman. (2002) The C-terminal domain of the largest subunit of RNA polymerase II is required for stationary phase entry and functionally interacts with the Ras/PKA signaling pathway. Journal of Biological Chemistry 277: 19488-19497. 16. Herman, P. K. (2002) Stationary phase in yeast. Current Opinion in Microbiology 5: 602-607. 17. Howard, S. C., A. Hester and P. K. Herman. (2003) The Ras/PKA signaling pathway may control RNA polymerase II elongation via the Spt4p/Spt5p complex in Saccharomyces cerevisiae. Genetics 165: 1059-1070. 18. Budovskaya, Y. V., J. S. Stephan, F. Reggiori, D. J. Klionsky and P. K. Herman. (2004) The Ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in Saccharomyces cerevisiae. Journal of Biological Chemistry 279: 20663-20671. 19. Bourbon, H. M., et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Molecular Cell 14: 553-557. [Letter to Editor] 20. Chang, Y.-W., S. C. Howard and P. K. Herman (2004) The Ras/PKA signaling pathway directly targets the Srb9 protein, a component of the general RNA polymerase II transcription apparatus. Molecular Cell 15: 107-116. 21. Budovskaya, Y. V., J. S. Stephan, S. J. Deminoff and P. K. Herman (2005) An evolutionary proteomics approach identifies novel substrates of the cAMP-dependent protein kinase. Proceedings of the National Academy of Sciences 102: 13933-13938. 22. Howard, S. C., S. J. Deminoff and P. K. Herman (2006) Increased phosphoglucomutase activity suppresses the galactose growth defect associated with elevated levels of Ras signaling in S. cerevisiae. Current Genetics 106: 1-6. 23. Stephan, J. S. and P. K. Herman. (2006) The regulation of autophagy in eukaryotic cells: Do all roads pass through Atg1? Autophagy 2: 146-148. 24. Deminoff, S. J., S. C. Howard, A. Hester, S. Warner and P. K. Herman. (2006) Using substrate- binding variants of the cAMP-dependent protein kinase to identify novel targets and a kinase domain important for substrate interactions in Saccharomyces cerevisiae. Genetics 173: 1909-1917. 25. Deminoff, S. J. and P. K. Herman. (2007) Identifying Atg1 substrates: four means to an end. Autophagy 3: 667-673. 26. Stephan, J. S., Y.-Y. Yeh, V. Ramachandran and P. K. Herman (2008) The cAMP-dependent and Tor protein kinases coordinately regulate autophagy activity via the Atg13 protein. Manuscript in preparation. 27. Deminoff, S. J., V. Ramachandran and P. K. Herman (2008) Distal recognition sites in substrates are required for efficient phosphorylation by the cAMP-dependent protein kinase. Manuscript submitted.

C. Research Support. National Institutes of Health (R01 GM65227) December 1, 2006 - November 30, 2010 Ras protein signaling and the control of cell growth. Role: P.I. Project aims to examine how the Ras/PKA signaling pathway in S. cerevisiae regulates autophagy and how the activity of this pathway is coordinated with that of the Tor and AMP-activated protein kinase pathways.

Comprehensive Cancer Center, The Ohio State University Collaborative seed grant July, 2008 – June, 2009 Identifying substrates of the cAMP-dependent protein kinase relevant to the pathology of Carney Complex. Role: P.I., co-P.I. Lawrence Kirschner Project aims to adapt a novel strategy for identifying protein kinase substrates for the mammalian cAMP- dependent protein kinase. The long-term goal is to gain insights into the mechanisms responsible for the increased tumor incidence associated with Carney Complex.

PHS 398/2590 (Rev. 09/04) Page Continuation Format Page

BIOGRAPHICAL SKETCH Hopper, Anita K. Professor EDUCATION/TRAINING INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY University of Illinois, Chicago B.S. 1967 Biology University of Illinois, Urbana Ph.D. 1972 Cell Biology University of Washington, Seattle Postdoctoral 1971-1975 Genetics A. Positions and Honors. Positions and Employment 1975-1978 Assistant Professor, Department of Microbiology, Univ. Mass. Med. Center, Worcester, MA 1978-1979 Associate Professor, Department of Microbiology, Univ. Mass. Med. Center, Worcester, MA 1979-1987 Associate Professor, Department of Biological Chemistry, Milton S. Penn. State Univ. Coll. Med., Hershey, PA 1987-2006 Professor, Biochemistry Molecular Biology, Penn. State Univ. Coll. Med., Hershey, PA 2006 Distinguished Professor, Biochem. Molecular Biology, Penn. State Univ. Coll. Med, Hershey, PA 2006 Professor & Chair, Molecular Genetics, The Ohio State University, Columbus, OH Other Experience and Professional Memberships 1981-1985 Grant Review Panels: NSF Genetic Biology 1985-1989 NIH Genetics Study Section 1986 Co-chairman – 5th Summer Symposium in Molecular Biology: The Nucleus at PSU 1986-1990 Editorial Boards: Molecular & Cellular Biology; RNA (1995-l997) 1987-1988 Chair-elect /Chair Genetics and Molecular Biology Division of the ASM 1989-1990 Co-organizer - CSH Meetings on RNA Processing 1989-2000 Editor: Molecular & Cellular Biology 1994-1996 ACS Scientific Advisory Comm 1995-1996 Sabbatical Visiting Scientist, Fred Hutchison Cancer Research Center, Seattle, WA 1996 Co-organizer - Keystone Symposium on Posttranscriptional Processing 1997-1998 Member ACS Genetic Mechanisms of Cancer Peer Review Comm. 1997-2002 Member/Acting Chair /Chair NIH Molecular Biology (CDF-1) Study Section 1998-2004 Co-Chair/Chair/past-Chair: Yeast Genetics and Molecular Biology Meeting 2001-2006 Co-chair: Pennsylvania State University Intercollege Graduate Program in Genetics 2002-2006 Co-director: Pennsylvania State University Huck Institutes IBIOS program 2008 Panel member – HHMI Investigator contest Honors 1967-1968 NIH Trainee in Cell Biology 1972-1974 NIH Postdoctoral Fellowship 1994-present Fellow of American Academy of Microbiologists 2003-2004 President RNA Society 2004-2007 Secretary Genetics Society of America 2004 & 2005 Overall Best Instructor – PSU Graduate Student selection 2005 Distinguished Educator PSU College Medicine 2006 Distinguished Professor Penn. State Univ. 2007 Distinguished NSF-Advance Lecturer – Case Western Reserve University B. Publications (Selected from 9 reviews & 75 peer-reviewed publications) Foundation work 1. Hopper, AK, F Banks, V Evangelides. A mutant of yeast which accumulates tRNA precursors. Cell 14:211- 219 (1978). 2. Hopper, AK, LD Schultz, RA Shapiro. Processing of intervening sequences: A new yeast mutant which fails to excise intervening sequences from precursor tRNAs. Cell 19:741-751 (1980). 3. Hopper, AK, A Furukawa, HD Pham, NC Martin. Defects in modification of cytoplasmic and mitochondrial transfer RNAs are caused by single nuclear mutations. Cell 28:543-550 (1982). 4. Hopper, AK, HM Traglia, RW Dunst. The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus. J. Cell Biol. 111:309-322 (1990). Selected publications last 10 years 1. Vaduva, G, NC Martin, AK Hopper. Actin-binding verprolin is a polarity development protein required for the morphogenesis and function of the yeast actin cytoskeleton. J. Cell Biol. 139:1821-1833 (1997). 2. Sarkar, S., AK Hopper. tRNA nuclear export in S. cerevisiae: in situ hybridization analysis. Mol. Biol. Cell 9:3041-3055 (1998). 3. Hopper, AK. Nuclear functions charge ahead. Science 282:2003-2004 (1998) - Review. 4. Hopper, AK Nucleus/cytosol exchange: inside out regulation. Current Biol. 9: R803-806 (1999) - Review.

1 Biographical Sketch Format Page

5. Feng, W-Q., A.L. Benko, J.-H. Lee, D.R. Stanford and A.K. Hopper. Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution. J. Cell Sci. 112:339-347 (1999). 6. Vaduva, G, I Anton, N Martinez-Quiles, NC Martin, R Geha, AK Hopper, N Ramesh The human WASP- interacting protein, WIP, activates the cell polarity pathway in yeast. J. Biol. Chem. 274:17013-17018 (1999). 7. Tolerico, LH, AL Benko, J-H Lee, DR Stanford, A.K. Hopper. S. cerevisiae Mod5p-II contains sequences for nuclear and cytosolic locations. Genetics 151:57-75 (1999). 8. Sarkar, S, AK Azad, AK Hopper. Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in S. cerevisiae. Proc. Natl. Acad. Sci. 96: 14366-14371 (1999). 9. Stanford, DR, NC Martin, AK Hopper. Information necessary for subcellular distribution of eukaryotic sorting isozymes resides in domains missing from eubacterial and archael counterparts. Nucl. Acids Res. 28: 383-392 (2000). 10. Benko, AL, G Vaduva, NC Martin, AK Hopper. Competition between a sterol biosynthetic enzyme and tRNA modification in addition to changes in the protein synthesis machinery cause altered nonsense suppression. Proc. Natl. Acad. Sci. 97: 61-66 (2000). 11. Qui, H, C Hu, J Anderson, GR Bork, S Sarkar, AK Hopper, A Hinnebusch. Defects in tRNA processing and nuclear export induce GCN4 translation independently of phosphorylation of the a subunit of eukaryotic translation initiation factor. Mol. Cell. Biol. 20: 2505-2516 (2000). 12. Azad, AK, DR Stanford, S Sarkar, AK Hopper. Role of nuclear pools of aminoacyl-tRNA synthetases in tRNA nuclear export. Mol. Biol. Cell. 12: 1381-1392 (2001). 13. Pluta, K, O Lefebvre, NC Martin, WJ Smagowicz, DR Stanford, SR Ellis, AK Hopper, A Sentenac, M Boguta. Maf1p a negative regulator of tRNA synthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 5031- 5040 (2001). 14. Feng, W, AK Hopper. A Los1p-independent pathway for nuclear export on intronless tRNAs in S. cerevisiae. Proc. Nat. Acad. Sci. 99: 5412-5417 (2002) Reviewed by Faculty of 1000. 15. Kaminska, J, B Gajewska, AK Hopper, T Zoladek. Rsp5p, a new link between the actin cytoskeleton and endocytosis in yeast S. cerevisiae. Mol. Cell. Biol. 22: 6946-6958 (2002). 16. Hopper, AK, EM Phizicky. tRNA transfers to the limelight. Genes Dev. 17: 162-180 (2003) - Review. 17. Stanford, DR, ML Whitney, RL Hurto, DM.Eisaman, W-C Shen, AK Hopper. Yeast Sol proteins function in tRNA nuclear export and carbohydrate metabolism. Genetics 168: 117-127(2004). 18. Shaheen, HH, AK Hopper. Retrograde movement of tRNAs from the cytosol to the nucleus in S. cerevisiae. Proc. Natl. Acad. Sci. 102:11290-11295 (2005) (“From the cover” article; Reviewed, Faculty 1000). 19. Murthi, A, AK Hopper. Genome-wide screen for inner nuclear membrane protein targeting: roles for N- acetylation and an integral membrane protein. Genetics 170:1553-1560 (2005). 20. Gu, W, RL Hurto, AK Hopper, EJ Grayhack, EM Phizicky. Depletion of yeast tRNAHis guanylyl-transferase Thg1p leads to uncharged tRNAHis with additional m5C. Mol. Cell. Biol. 25:8191-8201 (2005). 21. Kwapisz, M, P Cholbinski, AK Hopper, J-P Rousset, T Zoladek. Rsp5p dependent ubiquitination modulates translation accuracy in yeast Saccharomyces cerevisiae, RNA 11:1710-1718 (2005). 22. Hopper, AK. Cellular dynamics of small RNAs. Critical Reviews Biochem. Mol. Biol. 41: 3-19 (2006). 23. Butterfield-Gerson, KL, LZ Scheifele, EP Ryan, AK Hopper, LJ Parent. Importin-β family members mediate alpharetrovirus Gag nuclear entry via signals in matrix and nucleocapsid. J. Virology 80:1798-1806 (2006). 24. Engelke, DR, AK Hopper. Modified view of tRNA: Structural stability amid sequence diversity. Mol. Cell 21:144-145 (2006) - Review. 25. Hurto, RL, A Tong, C Boone, AK Hopper. Inorganic phosphate deprivation causes tRNA nuclear accumulation via retrograde transport in S. cerevisiae. Genetics 176: 841-852 (2007) - GSA highlighted article. 26. Whitney, ML, RL Hurto, HH Shaheen, AK Hopper. Rapid and reversible nuclear accumulation of cytoplasmic tRNA in response to nutrient availability. Mol. Biol. Cell 18: 2678-2686 (2007). 27. Shaheen, HH, RL Horetsky. SR Kimball, A Murthi, LS Jefferson, AK Hopper Cytoplasmic tRNA is imported into nuclei of rat hepatoma cells in response to nutrient deprivation. Proc. Natl. Acad. Sci. 104:8845-8850 (2007) - Reviewed by Faculty of 1000. 28. Weinert, T, AK Hopper. tRNA traffic meets a cell-cycle checkpoint. Cell 131: 838-839 (2007) - Review. 29. Hopper, AK, HH Shaheen. A decade of surprises for tRNA nuclear/cytoplasmic dynamics. Trends in Cell Biology 18:98-104 (2008) (peer evaluated commissioned review; journal featured article). 30. Stauffer*, KA, T-P Lai*, A Murthi, , G Peng, HH Shaheen, NC Martin, AK Hopper. A motif sufficient and necessary to target peripheral proteins to the yeast inner nuclear membrane. Submitted. (* equal first authors) C. Patent: A simple novel in vivo screen for alterations in flux through the sterol biosynthetic pathway. Inventors: AK Hopper, A.L. Benko, G Vaduva, NC Martin; Assignee: Penn. State Univ. (99-2085/2), issued 10/12/04.

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D. Research Support: (Ongoing) 2 R01 GM27930-27 (Anita K Hopper) NIH/NIGMS 09/01/06-6/30/10

"Yeast genes in RNA processing & nucleus/cytosol exchange" Role: Principal Investigator

3R01 GM27930-27S1 (Training for Ms. Greetchen Diaz) NIH/NIGMS 09/01/07-6/30/10 "Yeast genes in RNA processing & nucleus/cytosol exchange" Research Supplements to Promote Diversity in Health-Related Research Role: Principal Investigator

(Completed during the last 4 years) 3 R01 GM27930-25S1 (A.K. Hopper, PI) 07/01/04-12/31/06 NIH/NIGMS "Yeast genes in RNA processing & nucleus/cytosol exchange"

0315384U (A. Murthi, PI; AKH, Sponsor) 07/01/04-06/30/06 American Heart Association “Biogenesis and maintenance of the inner nuclear membrane: a genome-wide study”

No number assigned (S. Grigoryev, PI; AKH, Co-Investigator) 06/01/04-05/31/06 PA Tobacco Settlement Fund “Regulation of nuclear topography in cancer cells”

MCB-0115409 (A. Hopper & N. Martin, PIs) 09/01/01-02/28/04 National Science Foundation “Collaborative research: How single genes provide proteins to multiple cellular compartments”

0215299U (R. Hurto, PI; AKH, Sponsor) 07/01/02-06/30/04 American Heart Association “Getting to the heart of cardiac tRNA nuclear export using yeast”

3 Continuation Format Page Principal Investigator/Program Director (Last, First, Middle): HOPPER, James E

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE James E. Hopper Professor eRA COMMONS USER NAME JEHOPPER EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Wisconsin, Madison B.S. 1964 Natural Science University of Wisconsin, Madison M.S. 1967 Science Education University of Wisconsin, Madison Ph.D. 1970 Genetics University of Illinois, Urbana Postdoctoral 1969-71 Enzymology University of Washington, Seattle Postdoctoral 1971-75 Molecular Biology A. Positions and Honors Positions and Employment 1966-1969 Predoctoral NIH Genetics Trainee, Genetics Department, University of Wisconsin. 1969 NIH Research Trainee in the Fertilization and Gamete Physiology Program, Woods Hole, MA. 1969-1971 Postdoctoral training with Dr. David Dickinson, Plant Physiol. Program, University of Illinois. 1972-1975 NIH Postdoctoral Fellow, Dr. Ted Young Dept. Biochem., University of Washington 1975-1976 Instructor, Department of Microbiology, University Massachusetts Medical Center 1976-1979 Adjunct Assistant Professor, Rosenstiel Basic Science Research Center, Brandeis University 1979-1987 Associate Professor, Dept of Biochem & Mol Biol, College of Medicine, Penn State Univ. 1987-1988 Sabbatical Research at Zymogenetics, Inc., Seattle, WA 1987-2006 Professor, Dept of Biochem. & Mol. Biol, College of Medicine, Penn State Univ. 1995-1996 Sabbatical Research at the Fred Hutchinson Cancer Research Center, Seattle, WA 2006-present Professor, Dept of Biochem & Dept of Molecular Genetics. Ohio State University.

Other Experience and Professional Memberships 1980, Ad Hoc member NIH Biomedical Sciences Fellowship Review Committee 1986-1991 Editorial Board Membership, Journal of Bacteriology 1982, Ad Hoc member, NIH Molecular Biology Study Section 1986 Co-chairman, 5th Summer Symp. in Mol. Biol., “The Nucleus”, Penn State Univ., 1986, Ad Hoc member NIH Microbiology, Physiology and Genetics Study Section. 1987, Member, American Association Western Peer Review Committee, 1990, Member, NSF Special Projects Review Panel, Divisions of Cell & Molecular Biosciences 1991, Ad Hoc member NIH Biomedical Sciences Fellowship Review Committee. 1991-1997, Member NSF Microbial Genetics Grant Review Panel. 1992, Co-chairman, 11th Summer Symp. in Mol. Biol., “Cell Growth and Regulation”, Penn State Univ 1994-1995 Chairman, Penn. State Univ. Intercollege Program in Genetics 1999, Member, Organizing Committee, 18th Summer Symp. in Mol. Biol. “Chromatin Structure and DNA Function: 25 Years of the Nucleosome”, Penn State Univ. 1999- Member, Genetics Society of America

B. Selected peer-reviewed publications (in chronological order). Hopper, J.E., L.B. Rowe and J.R. Broach (1978). Regulation of the galactose pathway in Saccharomyces cerevisiae: Induction of uridyl-transferase-specific mRNA and dependency on GAL4 function. Proc. Natl. Acad. Sci. 75:2878-2882. Hopper, J.E. and L.B. Rowe (1978). Molecular expression and regulation of the galactose gene cluster in Saccharomyces cerevisiae. Messenger RNA sizes and GAL4 control of inducible mRNAs. J. Biol. Chem. 253:7566-7569. Perlman, D. and J.E. Hopper (1979). Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in Saccharomyces cerevisiae. Cell 16:89-95. Hopper, J.E., Bostian, K.A., Rowe, L.B. and Tipper, D.J. (1977).Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae. J. Biol. Chem. 252:9010-9017.

PHS 398/2590 (Rev. 09/04) Page 7 Biographical Sketch Format Page Principal Investigator/Program Director (Last, First, Middle): HOPPER, James E Bostian, K.A., J.E. Hopper and D.T. Rogers (1980). Translational analysis of the dsRNA genome of the killer- associated virus-like particles of Saccharomyces cerevisiae: M-dsRNA encodes toxin. Cell 19:403-414. Johnston, S.A. and J.E. Hopper (1982). Isolation of the yeast regulatory gene, GAL4, and analysis of its dosage effects on the galactose/melibiose regulon. Proc. Natl. Acad. Sci. USA 79:6971-6975. Torchia, T., R.W. Hamilton, C. Cano and J.E. Hopper (1984). Disruption of the regulatory gene GAL80 in yeast: effects on the carbon-controlled regulation of the gal/mel pathway genes. Mol. Cell. Biol. 4:1521-1527 Lohr, D. and J.E. Hopper (1985). The relationship of regulatory proteins and DNAse I hypersensitive sites in the yeast GAL1-10 genes. Nucleic Acids Res. 13:8409-8423. Johnston, S., M. Zavortink, C. Debouck, M. Rosenberg and J.E. Hopper (1986). Functional domains of the yeast regulatory protein GAL4. Proc. Natl. Acad. Sci. USA 83:6553-6557. Lohr, D., T. Torchia and J.E. Hopper (1987). The regulatory protein GAL80 is a determinant of the chromatin structure of the yeast GAL1-10 control region. J. Biol. Chem. 262:15589-15597. Bajwa, W., T. Torchia, J. Tschopp and J.E. Hopper (1988). The yeast regulatory gene, GAL3: carbon regulation; UASgal elements in common with GAL1, GAL2, GAL10, GAL80, and MEL1; and an encoded protein strikingly similar to yeast and E. coli galactokinases. Mol. Cell. Biol. 8:3439-3447. Mylin, L.M., J.P. Bhat and J.E. Hopper (1989). Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator. Genes and Development 3: 1157-1165. Bhat, J., D. Oh and J.E. Hopper (1990). Analysis of the GAL3 signal transduction pathway activating GAL4 protein dependent transcription in S. cerevisiae. Genetics 125:281-291. Mylin, L.M., M. Johnston and J.E. Hopper (1990). Phosphorylated forms of GAL4 are correlated with its ability to activate transcription. Mol. Cell. Biol. 10:4623-4629. Bhat, J.P. and J.E. Hopper (1991). The mechanism of inducer formation in gal3 mutants of the yeast galactose system is independent of normal galactose metabolism and mitochondrial respiratory function. Genetics 128:233-239. Bhat, J.P. and J.E. Hopper (1992). Overproduction of the GAL1 or GAL3 proteins causes galactose-independent activation of the GAL4 protein: Evidence for a new model of induction for the yeast GAL/MEL regulon. Mol. Cell. Biol. 12:2701-2707. Blank, T.E., M.P. Woods, C.M. Lebo, P. Xin, and J.E. Hopper (1997). Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 17:2566-2577. Sil,, A. K., Alam, S., Xin, P., Ma, L., Morgan, M., Lebo, C., Woods, M., Hopper, J. (1999). “The Gal3p-Gal80p- Gal4p transcription switch of yeast: Gal3p destabilizes Gal80p-Gal4p complex in response to galactose and ATP”. Mol. Cell Biol. 19:7828-7840. Sil, A. K., Xin, P., and Hopper, J. E. (2000). Vectors allowing amplified expression of the Saccharomyces cerevisiae Gal4p, Gal80p and Gal3p Transcription Switch: Applications to Galactose-Regulated High-Level Production of Proteins. Protein Expression and Purification 18:202-212. Cell Biol. 19:7828-7840. Peng, G. and. Hopper, J.E. (2000). Evidence for Gal3p’s Cytosolic Location and Gal80p’s Dual Cytosolic/Nuclear Location Suggests New Mechanisms for Controlling Gal4p Activity in Saccharomyces cerevisiae. Mol. Cell Biol. 20:5140-5148. Peng, G. and. Hopper, J.E.. (2002) Gene Activation by Interaction of an Inhibitor with a Cytoplasmic Signaling Protein Proc. Natl. Acad. Sci. USA. 99:8548-8553. Carrozza,, M.J., John, S., Sil,, A.K., Hopper, J. E. and Workman, J. L. (2002) Gal80 confers specificity on HAT complex interactions with activators: J. Biol. Chem. 277:24648-246-52. Meehan, W.J. Samant, R.S., Hopper, J.E., Carrozza, M.J., Shevde, L.S., Workman, J.L., Eckert, K.E. Verderame, M.F. and Welch, D.R. (2004) Breast Cancer Metastasis Suppressor 1 (BRMS1) Forms Complexes with Retinoblastoma-binding Protein 1 (RBP1) and the mSin3 Histone Deacetylase Complex and Represses Transcription. J.Biol. Chem. 279:1562-1569. Adams, C.A., Kar, S.R., Hopper, J.E., and Fried, M.G. (2004) Self-association of the Amino-terminal Domain of the Yeast TATA-binding Protein. J. Biol. Chem. 279: 1376-1382. Pilauri, V., Bewley, M., Diep, C., and Hopper, J. (2005). Gal80 Dimerization and the Yeast GAL Gene Switch. Genetics 169: 1903-1914. Diep, C., Peng, G., Bewley, M., Pilauri, V., Ropson, I., and Hopper, J. (2006) Intragenic Suppression of Gal3C Interaction with Gal80 in the Saccharomyces cerevisiae GAL Gene Switch. switch Genetics 113:229-246. Diep,C., Pilauri, V., Tao, X., Losiewicz, M., Blank, T., and Hopper, J. (2008) Genetic evidence for sites of interaction between the Gal3 and Gal80 proteins of the Saccharomyces cerevisiae GAL gene switch, Genetics 178:725-736.

PHS 398/2590 (Rev. 09/04) Page 8 Biographical Sketch Format Page Principal Investigator/Program Director (Last, First, Middle): HOPPER, James E

C. Research Support

Ongoing Research Support RO1 GM27925-23(NIH/GM ) James E. Hopper (PI) 4/1/2007-3/31/2011 “Molecular Basis of Interchromosomal Gene Regulation”. This study investigates the genetic and molecular mechanisms operating in a model transcriptional switch of the yeast, Saccharomyces cerevisiea. The goal is to determine how the three proteins, Gal3, Gal80 and Gal4 work to regulate galactose-responsive transcription activation of a family of nine target genes. Role: PI.

Completed Research Support RO1 GM23717 ** (NIH/GM) James E. Hopper (PI) 7/1/76 –6/30/79 “Molecular Basis of Interchromosomal Gene Regulation”. This study investigates the genetic and molecular mechanisms operating in a model transcriptional switch of the yeast, Saccharomyces cerevisiea. The goal is to determine how the three proteins, Gal3, Gal80 and Gal4 work to regulate galactose-responsive transcription activation of a family of nine target genes. Role: PI ** GM23717 changed in number designation to GM27925 upon move from Brandeis Univ. to Penn State Univ.

RO1 GM27925-22 (NIH/GM) James E. Hopper (PI) 9/1/79 – Present. “Molecular Basis of Interchromosomal Gene Regulation”. This study investigates the genetic and molecular mechanisms operating in a model transcriptional switch of the yeast, Saccharomyces cerevisiea. The goal is to determine how the three proteins, Gal3, Gal80 and Gal4 work to regulate galactose-responsive transcription activation of a family of nine target genes. Role: PI.

RO1 GM25092 (NIH/GM) James E. Hopper (PI) 4/1/78 –3/31/81 “Inter-Genomic Functional Relationships in Yeast” This studies goals were to map and decode genes of the yeast killer viral genomes to understand the relationships between killer viral encoded functions and yeast (Saccharomyces cerevisiae) nuclear gene functions in the establishment and maintenance of the killer phenotypes.

PHS 398/2590 (Rev. 09/04) Page 9 Biographical Sketch Format Page

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Lamb, Rebecca Sarah Assistant Professor Plant Cellular and Molecular eRA COMMONS USER NAME Biology

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Wesleyan University Middletown, CT BA 1992 Biology/German Studies Duke University Durham, NC Ph.D. 1998 Zoology and Cell and Molecular Biology

Yale University New Haven, CT NA 1998-2003 Floral development in Arabidopsis thaliana

A. Positions and Honors.

Positions and Employment 1998-2003 Postdoctoral Researcher, Yale University, New Haven, CT 2003- Assistant Professor, Plant Cellular and Molecular Biology, Plant Molecular Biology and Biotechnology Program, and The Molecular, Cellular, and Developmental Biology Program, The Ohio State University

Professional Memberships 1999- Member, American Society of Plant Biologists 2003- Member, American Society for the Advancement of Science 2003- Member, Genetics Society 2004- Member, Society for Developmental Biology 2004- Member, FASEB 2007- Member, Association for Women in Science

Professional Activities 2003- Reviewer, Plant Cell, Plant Science, PNAS, Plant Physiology, Development, Science, Nature 2005- Panelist, National Science Foundation, Epigenetics, Transcription and Chromatin Panel within the Genes and Genome Systems, BIO/MCB

Fellowships and Honors 1988-1992 Hedden Scholarship, Wesleyan University, Middletown, CT 1992 Blankennagel Prize in German, Wesleyan University, Middletown, CT 1992-1995 NIH Training Grant in Cell and Molecular Biology, Duke University, Durham, NC 1998-1999 Brown Postdoctoral Fellowship, Yale University, New Haven, CT

B. Selected peer-reviewed publications (in chronological order). 1. Morohashi, K, Zhao, M, Yang, M, Read, B, Lloyd, A, Lamb R, and Grotewold, E (2007). Participation of the Arabidopsis bHLH factor GL3 in trichome initiation regulatory events. Plant Physiology 145(3): 736-746. 2. Palaniswamy, S., James, S., Sun H, Lamb, R.S., Davuluri, R.V., Grotewold, E (2006). AGRIS and AtRegNet: A platform to link cis-regulatory elements and transcription factors into regulatory networks. Plant Physiology 140(3): 818-829. 3. Lamb, R.S. and Irish, V.F. (2003) Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages. Proc. Nat. Acad. Sci. 100: 6558-6563. 4. Lamb, R.S., Hill, T.A., Tan, Q.K., and Irish, V.F. (2002) Regulation of APETALA3 floral homeotic gene expression by meristem identity genes. Development 129: 2079-86. 5. Ward, R.E., Schweizer, L, Lamb, R.S., and Fehon, R.G. (2001) The protein 4.1, radixin, moesin (FERM) domain of Drosophila Coracle, a cytoplasmic component of the septate junction, provides functions essential for embryonic development and imagninal cell proliferation. Genetics 159: 219-228. 6. Lamb, R.S., Ward, R.E., Schweizer, L. and Fehon, R.G. (1998) Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in the embryonic and adult epithelial cell. Mol. Biol. Cell 9: 3505-3519. 7. Ward, R.E., Lamb, R.S., and Fehon, R.G. (1998) A conserved functional domain of Drosophila Coracle is required for localization at the septate junction and has membrane organizing activity. J. Cell Biol. 140: 1463-1473. 8. Fehon, R.G., LaJeunesse, D, Lamb, R.S., McCartney, B.M., Schweizer, L., and Ward, R.E. (1997) Functional studies of the protein 4.1 family of junctional proteins in Drosophila. Soc. Gen. Physiol. Ser. 52: 149-159.

C. Research Support

Ongoing Research Support

Ohio Plant Biotechnology Consortium Research Grant 7/1/2007-7/30/2009 Two Arabidopsis WWE-PARP Proteins Involved in Abiotic Stress Response and Development. The major goal of this project is characterize the phenotypic and molecular phenotypes of two Arabidopsis genes that appear to be involved in epigenetic control of transcription in plants. Role: Principal Investigator

Completed Research Support

MCB-0418891 Grotewold (PI) NSF 9/1/2004-8/31/2007 Arabidopsis 2010: Establishing Regulatory Networks in Arabidopsis: Integrating AGRIS with the Identification of Direct Targets for Transcription Factors The goal of this research was to determine the genes directly controlled by a set of plant transcription factors. Role: Co-investigator

Principal Investigator/Program Director (Last, first, middle): ,

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Leone, Gustavo W. Associate Professor

eRA COMMONS USER NAME gleone

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) University of Calgary, Calgary, Canada B.Sc. 1988 Biochemistry University of Calgary, Calgary, Canada Ph.D 1994 Molecular Virology Duke University Medical Center, Durham,N.C Postdoc 1994-1998 Genetics Ohio State University, Columbus, OH Assoc Prof 1999-present Cancer Genetics A. Positions and Honors. Academic Appointments 1987 – 1988 Undergraduate Research Assistant, Department of Microbiology and Infectious Diseases, University of Calgary (Supervisor: Dr. Patrick W.K. Lee) 1988 – 1994 Graduate Student, Department of Microbiology and Infectious Diseases, University of Calgary (Supervisor: Dr. Patrick W.K. Lee) 1990 – 1994 Graduate student assistant, University of Calgary, Project Lab (intensive laboratory training for undergraduates in virology and molecular biology 1994 – 1998 Postdoctoral Fellow, Department of Genetics, Duke University Medical Center (HHMI), Durham, North Carolina. Role of E2F in Cell Cycle Control (Mentor: Dr. Joseph R. Nevins) 1999–present Associate Professor, Human Cancer Genetics Program, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio. Honors and Awards 1989 – 1994 Alberta Heritage Foundation for Medical Research Studentship 1994 – 1997 Medical Research Council of Canada Fellowship and Alberta Heritage Foundation for Medical Research Fellowship 1997 – 1999 Medical Research Council of Canada Centennial Fellowship 1999 The Robert M. and Barbara R. Bell Basic Science of Cancer Award 1999 – 2001 Scholar Award, The 1999 V - Foundation 2001 – 2005 Scholar Award, PEW Charitable Trust 2004 – 2009 Scholar Award, Leukemia and Lymphoma Society B. Selected peer-reviewed publications (Selected from a total of 70)

1. Leone, G., Maybaum, L. And Lee, P.W.K. (1992). The Reovirus cell attachment protein possesses two independently active trimerization domains: Basis of dominant negative effects. Cell 71:479-488. 2. DeGregori, J.*, Leone, G.*, Ohtani, K., Miron, A. and Nevins, J.R. (1995) E2F1 accumulation bypasses a G1 arrest resulting from the inhibition of G1 cyclin-dependent kinase activity. Genes & Development, 9:2873-2887. *These authors contributed equally to this work. 3. Gilmore, R., Coffey, M., Leone, G., McLure, K., Lee, P.W.K. (1996). Co-translational trimerization of the Reovirus cell attachment protein. The EMBO Journal 15(11):2651-2658. 4. Leone, G., Coffey, M., Gilmore, R., Duncan, R., and Lee, P.W.K. (1996) C-terminal Trimerization, but not N- terminal trimerization, of the Reovirus cell attachment protein is a post-translational and hsp70/ATP dependent process. J. Biol. Chem. 271 (14):8466-8471. 5. DeGregori, J., Leone, G., Miron, A., Jakoi, L., and Nevins, J.R. (1997). Distinct roles for E2F proteins in cell growth control and apoptosis. Proc. Natl. Acad. Sci. USA. 94, 7245-7250.

Page _ 1_ Principal Investigator/Program Director (Last, first, middle): ,

6. Leone, G., DeGregori, J., Sears, R., Jakoi, L., and Nevins, J.R. (1997). Myc and Ras collaborate in inducing accumulation of active cyclin E/Cdk2 and E2F. Nature. 387, 422-425. 7. Leone, G., DeGregori, J., Yan, Z., Jakoi, L., Ishida, S., Williams, R.S., Nevins, J.R. (1998). E2F3 activity is regulated during the cell cycle and is required for the induction of S phase. Genes & Development. 12:2120- 2130. 8. Sears, R., Leone, G., DeGregori, J., and Nevins,.J.R. (1999). Ras enhances Myc protein stability. Molecular Cell. 3:169-179. 9. Leone, G., DeGregori, J., Jakoi, L., Cook, J.G., and Nevins, J.R. (1999). Collaborative role of E2F transcriptional activity and G1 cyclin-dependent kinase activity in the induction of S phase. Proc. Natl. Acad. Sci. USA . 96:6626-6631. 10. Leone, G., Nuckolls, F., Ishida, S., Adams, M., Sears, R., Jakoi, L., Miron, A., Nevins, J.R. (2000) Identification of a novel E2F3 product suggests a mechanism for determining specificity of repression by Rb proteins. Mol. Cell. Biol. 20:10, 3626-3632 11. Leone G., Sears R., Huang E., Rempel R., Nuckols F., Park C., Giangrande P., Wu L., Saavedra H.I., Field S.J., Thompson M.A., Yang H., Fujiwara Y., Greenberg M.E., Orkin S., Snith C., and Nevins J.R. (2001). Myc requires distinct E2F activities to induce S phase and apoptosis. Molecular Cell, 8: 105-113. 12.Wu L., Timmers C., Baidehi M., Saavedra H.I., Sang L., Chong G.T., Nuckolls F., Giangrande P., Wright F.A., Field S.J., Greenberg M.E., Orkin S., Nevins J.R., Robinson M.L., and Leone G. (2001). The E2F1-3 transcription factors are essential for cellular proliferation. Nature, 414:457-462. 13. Cook J.G., Park C., Burke T.W., Leone G., DeGregori J., Engel A., and Nevins J.R. (2002). Analysis of Cdc6 function in the assembly of mammalian prereplication complexes. Proc. Natl. Acad. Sci..USA, 99:3 1347-1352. 14. Saavedra H.I., Wu L., de Bruin A., Timmers C., Rosol T.R., Weinstein M., Robinson M.L, and Leone G. (2002). Specificity of E2F1, E2F2 and E2F3 in mediating phenotypes induced by loss of Rb. Cell Growth & Differentiation (13:5 215-225). Cover of Journal. 15. Wu L., de Bruin A., Saavedra HI., Trimboli A., Yang Y., Opavska J., Wilson P., Starovic M., Ostrowski MC., Cross JC., Weinstein M., Rosol TJ., Robinson ML., and Leone G. (2003). Extraembryonic function of Rb is essential for embryonic development and viability. Nature 421: 942-947. (N&V; 421: 903-004) 16. Saavedra HI., Maiti B., Timmers C., Altura R., Fukasawa K., and Leone G.. (2003). Inactivation of E2F3 results in premature centriole separation and centrosome amplification. Cancer Cell 3: 333-346 (Cover). 17. de Bruin A., Wu L., Saavedra H.I., Wilson P., Yang Y., Weinstein M., Rosol T.J., Robinsom M.L., Leone G. (2003). Rb function in extraembryonic lineages is critical for the control of apoptosis in the central nervous system of Rb-deficient mice. Proc. Natl. Acad. Sci..USA, 100:11 6546-6551. 18. de Bruin A, Maiti B, Jakoi L, Timmers C, and Leone G. (2003). Identification and Characterization of E2F7, a Novel Mammalian E2F Family Member Capable of Blocking Cellular Proliferation. J. Biol. Chem. 278:43, 42041-42049. 19. Zhang J, Gray J, Wu L, Leone G., Rowan S, Cepko CL, Zhu X, Craft CM, Dyer MA. (2004). Rb regulates proliferation and rod photoreceptor development in the mouse retina. Nature Genetics 36:4 1-10. 20. Jiang Y., Saavedra HI., Holloway MP., Leone G., Altura RA. (2004). Aberrant Regulation of Survivin by the Rb/E2F Family of Proteins. J. Biol. Chem 279, 40511-40520. 21. Iavarone A., King ER., Dai X., Leone G., Stanley ER., Lasorella A. (2004). Retinoblastoma promotes definitive erythropoiesis by repressing Id2 in fetal liver macrophages. Nature 432: 1040-1045. 22. Maiti B., Li J., de Bruin A., Gordon F., Timmers C, Opavsky R., Patil K., Tuttle J., Cleghorn W.,and Leone G. (2005). Cloning and Characterization of Mouse E2F8, a Novel Mammalian E2F Family Member Capable of Blocking Cellular Proliferation. J. Biol. Chem. 280, 18211-18220. 23. Logan N., Graham A., Zhao X., Fisher R., Maiti B., Leone G., La Thangue N. (2005). E2F-8: an E2F family member with a similar organization of DNA binding domains to E2F-7. Oncogene 24,5000-5004. 24. Sharma, N., Timmers, C., Trikkha, P., Saavedra, H., Obery, A., and Leone, G. (2006). Control of the p53-p21 CIP1 axis by E2f1, E2f2 and E2f3 is essential for G1/S progression and cellular transformation. J. Biol. Chem 281:36124-31. 25. Dorrance, A, Liu, S., Yuan, W., Becknell, B., Arnoczky, K., Guimond, M., Strout, M., Feng, L., Nakamura, T., Yu, L., Rush, L., Weinstein, M., Leone, G., Wu, L., Ferketich, A., Whitman, S., Marcucci, G., and Caligiuri, M., (2006). The Mll partial tandem duplication incudes aberrant Hox expression in vivo via specific epigenetic alterations. J. Clin. Invest. 116:65-78. 26. Timmers C., Opavsky R., Maiti B., Wu L., Wu J., Orringer D., Sharma N., Saavedra HI., Leone G. (2007). E2F1- 3 controls a p53-dependent checkpoint that is essential for cellular proliferation. Mol Cell Biol. 1:65-78.

Page _ 2_ Principal Investigator/Program Director (Last, first, middle): ,

27. Wenzel, P., Wu, L., de Bruin, A., Chong, J-L, Chen, W-Y., Dureska, G., Sites, E., Pan, T., Sharma, A., Huang, K.,Ridgway R., Mosaliganti K., Sharp, R., Machiraju, R., Saltz, J., Yamamoto, H., Cross, J., Robinson, M., and Leone, G. (2007). Rb is critical in a mammalian tissue stem cell population. Genes & Development 21:85-97. 28. Wenzel, P., Leone, G. (2007). Expression of Cre recombinase in early diploid trophoblast cells of the mouse placenta. Genesis. 45:129-34. 29.McClellan K., Ruzhynsky V., Douda D., Vanderluit J., Ferguson K., Chen D., Bremner R., Park D., Leone G., and Slack R. (2007). Unique requirement for Rb/E2F3 in neuronal migration: evidence for cell cycle-independent functions. Mol Cell Biol 27:4825-43. 30.Chen D., Opavsky R., Pacal M., Tanimoto N., Wenzel P., Seeliger M., Leone G., and Bremner R. (2007). Rb- mediated neuronal differentiation through cell cycle independent regulation of E2f3a. PLoS Biology 5:e179 1- 16. 31. Opavsky R., Wang S-H., Trikha P., Raval A., Huang Y., Wu Y-A., Rodriguez B., Keller B., Liyanarachi S., Wei G., Davuluri R., Weinstein M., Felsher D., Ostrowski M.C., Leone G*, and Plass C*. (2007). CpG island methylation in cancer is driven by the genetic configuration of tumor cells. PLoS Genetics 3:1757-69. *corresponding authors 32.Opavsky R., Tsai S-Y., Guimond M., Arora A., Opavska J., Becknell B., Kauffman M., Walton N., Stephens J., Fernandez S., Muthusamy N., Felsher W., Porcu P., Caligiuri M., and Leone G. (2007). Specific tumor suppressor function for E2F2 in Myc-induced T cell lymphomagenesis. Proc. Natl. Acad. Sci..USA 104:39, 15400-15405. 33. Saenz-Robles M., Markovics J.A., Chong J-L., Opavsky R., Whitehead R.H., Leone G., Pipas J.M. (2007). Intestinal hyperplasia induced by SV40 large tumor antigen requires E2F2. J. Virology 81:13191-9. 34. Trimboli, A., Fukino, K., deBruin, A., Wei, G., Shen, L., Tanner, S.M., Rosol, T.J., Robinson, M.L., Eng, C., Ostrowski, M.C., and Leone, G. (2008) Direct evidence for epithelial-mesenchymal transitions in breast cancer. Cancer Research. 68:(3) 937-945. 35.Li J., Ran C., Li E., Gordon F., Siddiqui H., Cleghorn W., Chen H., Pandit S., Khanizadeh M., Weinstein M., Leone G*., and de Bruin A. (2008). Synergistic function of E2F7and E2F8 is essential for cell survival and embryonic development. Dev. Cell 14:62-75. *corresponding author 36. Tsai S-Y., Opavsky R., Sharma N., Wu L., Naidu S., Nolan E., Feria-Arias E., Timmers C., Opavska J., de Bruin A., Chong J-L., Trikha P., Fernandez S., Stromberg P., Rosol JR., Leone G. Mouse development with a single E2F activator. Nature 454:1137-41. 37. Chong JL, Tsai S-Y, Sharma N, Opavsky R, Price R, Wu L, Fernandez SA and Leone G. (2008). E2f3a and E2f3b contribute to the control of cell proliferation and mouse development. Mol. Cell. Biol. (in press)

C. Research Support. (Current; does not include Training Grants)

1. 01/01/07-12/31/13 NIH/NCI 1 R01 CA121275- Title: E2F7 & E2F8 in the control of transcription and cellular proliferation PI: Gustavo Leone, PhD

2. 8/01/07-7/30/09 DOD BC061825 Title: Role of PTEN in the Tumor Microenvironment PI: Gustavo Leone, PhD Co-PI: Joel Saltz, PhD

3. 04/01/04-03/30/09 NIH/NICHHD RO1 HD047470 Title: E2F3 and Embryonic Development PI: Gustavo Leone, Ph.D.

4. 07/1/04-06/31/09 NCI P01 CA97189 Title: Genetic Analysis of the breast tumor microenvironment. PI: Michael Ostrowski, PhD Project 2 Leader: Gustavo Leone, Ph.D.

5. 12/31/04-12/31/09 Lymphoma & Leukemia Society Scholar Program Title: Mechanisms of MYC-induced Lymphomagenesis PI: Gustavo Leone, Ph.D. Page _ 3_

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.

NAME Professor of Plant Cellular and Molecular Biology

Meier, Iris

POSITION TITLE

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) Technical University M.S. 1984 Microbiology Darmstadt, Germany University of Duesseldorf, Ph.D. 1987 Molecular Biology Germany

Max Planck Institute for Postdoc. 1987-1990 Plant Molecular Biology Plant Breeding, Cologne, Germany. Postdoc. University of California at Postdoc. 1990-1993 Plant Molecular Biology Berkeley, CA

A. Positions and Honors.

Positions and Employment 1993 - 1996 Junior Group Leader, Applied Plant Molecular Biology, University of Hamburg, Germany. 1997 - 1999 Senior Research Scientist, DuPont Central Research and Development, Wilmington, DE. 1999 – 2003 Assistant Professor, Dept. of Plant Biology, Ohio State University. 2003 – 2008 Associate Professor, Dept. of Plant Cellular and Molecular Biology, Ohio State University. 2008- Professor, Dept. of Plant Cellular and Molecular Biology, Ohio State University.

Other Experience and Professional Memberships 1999- Ad hoc grant reviewer for: NSF, USDA, DOE, BBSRC (UK), The Leverhulme Trust (UK), BARD (The United States - Israel Binational Agricultural Research & Development Fund). 1999- Reviewer: The Plant Cell, The Plant Journal, Plant Physiology, Journal of Cell Biology, Transgenic Research, Planta, Plant Molecular Biology, Trends in Plant Sciences, Nucleic Acids Research, Traffic, Biotechniques, Plant Biology. 2001- Panel member: National Science Foundation, Cellular Organization Panel; United States Department of Agriculture CSREES, Developmental Processes of Crop Plants Panel; BARD (The United States – Israel Binational Agricultural Research and Development Fund): Model Systems and Functional Biology Panel. 2002 - 2003 International Advisory Committee: Society for Experimental Biology (SEB) meeting "The Nuclear Envelope: Signaling, Communication and Gene Regulation", 2003, Durham, UK. 2004 - Advisory Board: NSF Plant Genome project, “Global analysis of functional units in plant chromosomes: DNA replication, domain structure, and transcription.” 2006 - Associate Editor for Plant Molecular Biology 2006 – 2008 Co-Organizer, Nuclear envelope and Golgi, Cell Biology Satellite, SEB Marseille 2008

Honors 1984 - 1986 Ph.D. fellowship by the “Fond der Chemischen Industrie”, Germany 1988 - 1990 Post-doctoral fellowship by the Max-Planck-Society, Germany 1992 - 1993 Research Fellow of the German Science Foundation (DFG) 2006 Dean’s Award for Excellence in Research and Graduate Education, College of Biological Sciences, OSU

B. Selected peer-reviewed publications (selected from 52 research publications) Meier, I., Wray, L.V., Jr., and Hillen, W. (1988). Differential regulation of the Tn10 encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2. EMBO J. 7, 567-572. van de Löcht, U., Meier, I., Hahlbrock, K., and Somssich, I. E. (1990). A 125 bp promoter fragment is sufficient for strong elicitor-mediated gene activation in parsley. EMBO J. 9, 2945-2950. Meier, I., Hahlbrock, K., and Somssich, I. E. (1991). Elicitor-inducible and constitutive in vivo DNA footprints indicate novel cis-acting elements in the promoter of a parsley gene encoding pathogenesis-related protein 1. Plant Cell 3, 309-315. Meier, I., Callan, K. L., Fleming, A. J., and Gruissem, W. (1995). Organ-specific differential regulation of a promoter subfamily for the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit genes in tomato. Plant Physiol. 107, 1105-1118. Meier, I., Phelan, T., Gruissem, W., Spiker, S., and Schneider, D. (1996). MFP1, a novel plant filament- like protein with affinity for matrix attachment region DNA. Plant Cell 8, 2105-2115. Rose, A., Meier, I., and Wienand, U. (1999). The tomato I-box binding factor LeMYBI is a member of a novel class of Myb-like proteins. Plant J. 20, 641-652. Gindullis, F., and Meier, I. (1999). Matrix Attachment Region Binding Protein MFP1 Is Localized in Discrete Domains at the Nuclear Envelope. Plant Cell 11, 1117-1128. Gindullis, F., Peffer, N.A., and Meier I. (1999). MAF1, a novel plant protein interacting with MAR- binding protein MFP1 is located at the nuclear envelope. Plant Cell 11, 1755-1767. Rose, A. and Meier, I. (2001) A domain unique to plant RanGAP is responsible for its targeting to the plant nuclear rim. Proc. Natl. Acad. Sci. U.S.A. 98, 15377-15382. Calikowski, T.T., Meulia, T., and Meier, I. (2003) A Proteomic Study of the Arabidopsis Nuclear Matrix. J. Cell. Biochem. 90, 361-378. Rose, A., Manikantan, S., Schraegle, S., Maloy, M., Stahlberg, E. and Meier, I. (2004) Genome-wide Identification of Arabidopsis Coiled-coil Proteins and Establishment of the ARABI-COIL Database. Plant Physiol. 134: 927-939. Patel S, Rose A, Meulia T, Dixit R, Cyr RJ, Meier, I. (2004). Arabidopsis WPP-Domain Proteins Are Developmentally Associated with the Nuclear Envelope and Promote Cell Division. Plant Cell 16:3260-3273. Jeong, SY, Rose S, Joseph, J, Dasso, M, and Meier, I. (2005). Plant-specific mitotic targeting of RanGAP requires a functional WPP domain. Plant J 42:270-82. Rose A, Schraegle SJ, Stahlberg EA and Meier, I (2005). Coiled-coil protein composition of 22 proteomes - differences and common themes in subcellular infrastructure and traffic control. BMC Evol. Biol 5:66. Xu X, Rose A, Muthuswamy S, Jeong S-Y, Venkatakrishnan S, Zhao Q, and Meier I. (2007). NUCLEAR PORE ANCHOR, the Arabidopsis Homolog of Tpr/Mlp1/Mlp2/Megator, Is Involved in mRNA Export and SUMO Homeostasis and Affects Diverse Aspects of Plant Development. Plant Cell 19: 1537-1548. Xu X, Meulia T and Meier I. (2007). Anchorage of Plant RanGAP to the Nuclear Envelope Involves Novel Nuclear-Pore-Associated Proteins. Curr Biol. 17: 1157-1163. Zhao, Q, Brkljacic, J, and Meier, I. (2008) Two distinct, interacting classes of nuclear envelope-associated coiled-coil proteins are required for the tissue-specific nuclear envelope targeting of Arabidopsis RanGAP. Plant Cell 20, 1639-1651. Xu, X, Zhao, Q, Rodrigo-Peiris, T, Brkljacic, J, He, C, Mueller, S and Meier I. (2008) RanGAP1 is a continuous marker of the Arabidopsis cell division plane. Proc. Natl. Acad. Sci. USA, in press. Xu XM and Meier I. (2008). The Nuclear Pore Comes to the Fore. Trends in Plant Sci. 13, 1-50. Brkljacic J and Meier I (2008). The nuclear pore and plant development. Curr. Opin. Plant Biol., in press.

C. Research Support

Ongoing Research Support 04/07 – 03/10 National Science Foundation -Molecular and Cellular Biosciences -0641271 “Arabidopsis as a new experimental platform to investigate the function of the nuclear pore protein Tpr in SUMOylation and mRNA export.” Principle investigator. The objective of this grant is the functional investigation of the plant nucleoporin NUA/Tpr and how it relates to the connection between SUMOylation and nuclear RNA export.

Completed Research Support 04/08-03/09 National Science Foundation Molecular and Cellular Biosciences - 0805009 Group travel proposal: Nuclear Envelope and Golgi, Marseille 2008. Principle investigator. The purpose was to bring together US and European researchers to discuss connections between the nuclear envelope and Golgi in plants. 05/04 - 04/08 National Science Foundation -Molecular and Cellular Biosciences -0343167 "Investigating Structure, Function, and Evolution of a Plant-Specific Nuclear-Targeting Domain" Principle investigator. The overall goal of this grant was to understand the molecular mechanism of subcellular anchoring of plant RanGAP and how it relates to the functions of the Ran cycle in plant interphase and mitosis. 09/02 – 08/06 National Science Foundation - Molecular and Cellular Biosciences- 0209339: “Arabidopsis 2010: Investigating coiled-coil proteins in the Arabidopsis ORFeome” Principle investigator. The purpose of this project was to computationally identify all long coiled-coil proteins in Arabidopsis and functionally investigate a subgroup associated with the nucleus. 09/01 - 08/05 USDA Plant Growth and Development: “Anchoring of Ran-mediated signal transduction in plants”. Principle Investigator. The purpose of this research was to identify the unique aspects of RanGAP subcellular localization in plants. 09/01 - 08/05 National Science Foundation - Molecular and Cellular Biosciences 0079507: “Investigating the role of novel nuclear envelope-associated plant proteins in nuclear dynamics” The main goal of this work was to characterize a plant-unique mechanism of nuclear envelope targeting and identify proteins involved in the mechanism.

Principal Investigator/Program Director (Last, first, middle): Osmani, Stephen A.

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES. NAME POSITION TITLE Osmani, Stephen A., Ph.D. Professor and Ohio Eminent Scholar, Department of Molecular Genetics EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) Polytechnic of the Sough Bank, London, UK HND 1977 Applied Biology Kings College, London, UK Ph.D. 1984 Biochemistry A. Positions and Honors Academic Appointments 1977-1979 Membership of the Institute of Biology for Microbiology, Trent Polytechnic, Nottingham, UK 1984-1988 Post-doctoral Fellow, Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Camden, NJ 1988-1992 Assistant Professor, Department of Cell Biology, Baylor College of Medicine, Houston, TX 1992-1997 Staff Scientist, Geisinger Clinic, Penn State College of Medicine, Henry Hood Research Program, Weis Center for Research, Danville, PA 1997-2000 Professor, Cellular and Molecular Physiology, Penn State College of Medicine, Henry Hood Research Program, Weis Center for Research, Danville, PA 2000-2001 Senior Staff Scientist, Geisinger Clinic, Penn State College of Medicine, Henry Hood Research Program, Weis Center for Research, Danville, PA 2001-present Professor, Department of Molecular Genetics, The Ohio State University, Columbus, OH

Honors and Awards 1977 Distinction in Microbiology for Higher National Diploma, Polytechnic of the South Bank, London, UK 1985 Tadion-Rideal Prize for Distinguished Post-Graduate Work in the Molecular Sciences, University of London, London, UK 2001 Appointed Ohio Eminent Scholar, The Ohio State University, Columbus, OH 2006 Elected Fellow of the American Association for the Advancement of Science

Selected Recent Additional Professional Activities 1992-1996 Member, Science Research Council for Geisinger Clinic 1996-2001 Chairman, Weis Center for Research Safety Committee 1999-2003 Member, NIH Cell Development and Function Study Section (6) 2001- Editorial Board, Eukaryotic Cell 2005- Editorial Advisory Board, Molecular Microbiology 2007- Member, NIH Nuclear Dynamics and Transport Study Section

Reviewer for many Journals including: EMBO J, Fungal Genetics and Biology, Genetics, Journal of Bacteriology, Journal of Biological Chemistry, Journal of Cell Science, Molecular and Cellular Biology, Molecular Biology of the Cell, Molecular Microbiology, Trends in Cell Biology, Journal of Cell Science, Biochimica et Biophysica Acta (BBA)/Molecular Cell Research, Science

Grants reviewed for: National Institute of Health, PA Academy of Science Research Grant, Whitaker Foundation, National Science Foundation, North Carolina Biotech. Center, Cancer Research Campaign UK, Cancer Research Fund UK

B. Selected Publications (selected from a total of over 60 publications) Osmani SA, Osmani AH, Morris NR. The molecular cloning and identification of a gene product specifically required for nuclear movement in Aspergillus nidulans. J Cell Biol, 111:543-551, 1990. Osmani AH, O’Donnel K, Pu RT, Osmani SA. Activation of the nimA protein kinase plays a unique role in mitosis that cannot be bypassed by the bimE checkpoint. EMBO J, 10:2669-2679, 1991. Osmani AH, McGuire SL, Osmani SA. Parallel activation of the NIMA and p34cdc2 cell cycle regulated protein kinases is required to initiate mitosis in Aspergillus nidulans. Cell, 67:283-291, 1991. O’Connell M, Osmani SA, Morris NR, Osmani AH. Increased expression of cyclin B (nimEcyclinB) elevates pre-MPF in Aspergillus nidulans which suppresses mutation of nimTcdc25. EMBO J, 11:2139-2149, 1992. Lu KP, Osmani SA, Osmani AH, Means AR. Essential roles for calcium and calmodulin for G2/M progression in Aspergillus nidulans. J Cell Biol, 121:621-630, 1993. PHS 398 (Rev. 05/01) Page 1 of 3 Biographical Sketch

Principal Investigator/Program Director (Last, first, middle): Osmani, Stephen A.

Lu KP, Osmani SA, Means AR. Properties and regulation of the cell cycle specific protein kinase from Aspergillus nidulans. J Biol Chem, 268:8769-8776, 1993. Osmani AH, van Peij N, Mischke M, O’Connell MJ, Osmani SA. A single p34cdc2 protein kinase (encoded by nimXcdc2) is required at G1 and G2 in Aspergillus nidulans. J Cell Sci, 107:1519-1528, 1994. Xiang S, Xu G, Pu R, Fincher R, McGuire S, Osmani A, Osmani SA. The NIMA protein kinase is hyperphosphorylated and activated downstream of p34cdc2/cyclin B: coordination of 2 mitosis-promoting kinases. EMBO J, 14:986-94, 1995. Pu RT, Osmani SA. Mitotic destruction of the cell cycle regulated NIMA protein kinase of Aspergillus nidulans is required for mitotic exit. EMBO J, 14:995-1003, 1995. Xin X, Osmani AH, Osmani SA, Xin M, Morris NR. NudF, a nuclear migration gene in Aspergillus nidulans, is similar to the human LIS-1 gene required for neuronal migration. Mol Biol of the Cell, 6:297-310, 1995. Pu RT, Xu G, Wu LV, O’Donnell K, Ye XS, Osmani SA. Isolation of a functional homolog of the cell cycle specific NIMA protein kinase and functional analysis of conserved residues. J Biol Chem, 271:18110-18116, 1995. Ye XS, Fincher RR, Tang A, O’Donnell K, Osmani SA. Two S-phase checkpoints inhibit NIMA kinase and mitosis via BIME and Tyr15 phosphorylation of p34cdc2. EMBO J, 15:3599-3610, 1996. Osmani SA, Ye XS. Cell cycle regulation in Aspergillus by two protein kinases. Biochem J, 317:633-641, 1996. Ye XS, Fincher AT, Tang A, Osmani SA. The G2/M DNA damage checkpoint inhibits mitosis through Tyr15 phosphorylation of p34cdc2 in Aspergillus nidulans. EMBO J, 16:182-192. Martin M, Osmani SA, Oakley BR. The role of λ-tubulin in mitosis and cell cycle progression in Aspergillus nidulans. J Cell Sci, 110:623-633, 1997. Osmani SA, Ye XS. Targets of checkpoints controlling mitosis. Trends in Cell Biol, 7:283-288, 1997. Renzi L, Gersch MS, Cambell MS, Osmani SA, Wu L, Gorbsky GL. MPM-2 antibody-reactive phosphorylation can be created in detergent-extracted cells by kinetochore-bound and soluble kinases. J Cell Sci, 110:2013-2025, 1997. Ye XS, Fincher RR, McNeal KK, Gygax SE, Wexler AN, Ryan KB, James SW, Osmani SA. Proteolysis and tyrosine phosphorylation of MPF: the role of MCM2 and the initiation of DNA replication to allow tyrosine phosphorylation of cdc2. J Biol Chem, 272:33384-33393, 1997. Bussink HJ, Osmani SA. A CDK family member (phoAPH085) is required to link developmental decisions to environmental conditions in Aspergillus nidulans. EMBO J, 17:3990-4003, 1998. Wu L, Osmani SA, Mirabito PM. A role for NIMA in the nuclear localization of the B-type cyclin, NIMA, in Aspergillus nidulans. J Cell Biol, 141:1575-1587, 1998. Ye XS, Fincher AT, Tang A, Osmani AH, Osmani SA. Regulation of the APC/C by bimAAPC3 and proteolysis of NIMA. Mol Biol Cell, 11:3019-3030, 1998. Bussink HJ, Osmani SA. A MAP kinase (MPKA) is involved in polarized growth in the filamentous fungus Aspergillus nidulans. FEMS Micro, 173:117-125, 1999. Osmani AH, May GS, Osmani SA. The extremely conserved pyroA gene of Aspergillus nidulans is required for pyridoxine synthesis and indirectly for resistance to photosensitizers. J Biol Chem, 274:23565-23569, 1999. DeSouza CPC, Ye XS, Osmani SA. Checkpoint defects leading to premature mitosis cause endoreplication of DNA in Aspergillus nidulans. Mol Cell Biol, 10:3661-3674, 1999. Ye XS, McGuire SL, Wolkow T, Tang A, Fincher R, Hamer JE, Osmani SA. Interaction between developmental and cell cycle regulators is required for morphogenesis in Aspergillus nidulans. EMBO J, 18:6994-7001, 1999. DeSouza CPC, Wu L, Spotts J, Osmani AH, Osmani SA. Mitotic histone H3 phosphorylation by the NIMA kinase in Aspergillus nidulans. Cell, 102:293-302, 2000. Ovechkina Y, Maddox P, Oakley CE, Xiang X, Osmani SA, Salmon ED Oakley BR. Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus. Mol Biol Cell, 14:2192-2200, 2003. Osmani AH, Davies J., Oakley CE, Oakley BR, Osmani SA. TINA Interacts with the NIMA Kinase in Aspergillus nidulans and Negatively Regulates Astral Microtubules during Metaphase Arrest. Mol Biol Cell, 14:3169-3179, 2003. Galagan J. et al. The Genome Sequence of the Filamentous Fungus Neurospora crassa. Nature, 422:859-868, 2003. Dou X, Wu D, An W. Davies J, Hashmi, SB, Ukil L, Osmani SA. The PHOA and PHOB cyclin dependent kinases perform an essential function in Aspergillus nidulans. Genetics, 165:1105-15, 2003. De Souza C, Horn K, Masker, K Osmani SA. The SONB NUP98 nucleoporin interacts with the NIMA kinase in Aspergillus nidulans. Genetics, 165:1071-81, 2003. Prigozhina NL, Oakley CE, Lewis AM, Nayak T, Osmani SA, Oakley BR. γ-Tubulin Plays an Essential Role in the Coordination of Mitotic Events. Mol Biol Cell, 15:1374-1386, 2004. Osmani SA, Mirabito PM. The early impact of genetics on our understanding of cell cycle regulation in Aspergillus nidulans. Fungal Genet Biol, 41:401-10, 2004. Wu D, Dou X, Hashmi SB, Osmani SA. The Pho80-like cyclin of Aspergillus nidulans regulates development independently of its role in phosphate acquisition. J. Biol. Chem. 279: 37693-703, 2004 Yang, L, Ukil, L, Osmani, AH, Nahm, F, Davies, J, De Souza, CPC, Dou, X, Perez-Balaguer A, and Osmani SA Rapid production of gene replacement constructs and generation of a GFP-tagged centromeric marker in Aspergillus nidulans. Eukaryotic Cell 3:1359-62, 2004 De Souza CPC, Hashmi SB, Osmani AH, Osmani SA Partial nuclear pore complex disassembly during closed mitosis in Aspergillus nidulans. Current Biology 14:1973-84, 2004

PHS 398 (Rev. 05/01) Page 2 of 3 Biographical Sketch

Principal Investigator/Program Director (Last, first, middle): Osmani, Stephen A.

Davies JR, Osmani AH, De Souza CP, Bachewich C, Osmani SA. Potential link between the NIMA mitotic kinase and nuclear membrane fission during mitotic exit in Aspergillus nidulans. Eukaryotic Cell 3:1433-44, 2004 Bachewich C, Masker K, Osmani S. The polo-like kinase PLKA is involved in initiation and progression through mitosis in the filamentous fungus Aspergillus nidulans. Molecular Microbiology. 55: 572-587, 2005 Galagan JE, Calvo SE, Cuomo C, Ma LJ, Wortman JR, Batzoglou S, Lee SI, Basturkmen M, Spevak CC, Clutterbuck J, Kapitonov V, Jurka J, Scazzocchio C, Farman M, Butler J, Purcell S, Harris S, Braus GH, Draht O, Busch S, D'Enfert C, Bouchier C, Goldman GH, Bell-Pedersen D, Griffiths-Jones S, Doonan JH, Yu J, Vienken K, Pain A, Freitag M, Selker EU, Archer DB, Penalva MA, Oakley BR, Momany M, Tanaka T, Kumagai T, Asai K, Machida M, Nierman WC, Denning DW, Caddick M, Hynes M, Paoletti M, Fischer R, Miller B, Dyer P, Sachs MS, Osmani SA, Birren BW Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature. 438, 1105-15, 2005 Nayak T, Szewczyk E, Oakley CE, Osmani A, Ukil L, Murray SL, Hynes MJ, Osmani SA, Oakley BR. A versatile and efficient gene targeting system for Aspergillus nidulans. Genetics. 172,1557-66. 2005 De Souza CP, Hashmi SB, Horn KP, Osmani SA. A point mutation in the Aspergillus nidulans sonBNup98 nuclear pore complex gene causes conditional DNA damage sensitivity. Genetics. 174, 1881-93. 2006 Osmani AH, Davies J, Liu HL, Nile A, Osmani SA. Systematic Deletion and Mitotic Localization of the Nuclear Pore Complex Proteins of Aspergillus nidulans. Mol. Biol. Cell. 17, 4946-61. 2006 Osmani AH, Oakley BR, Osmani SA. Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique. Nature Protocols 1, 2517-2526, 2006 Szewczyk E, Nayak T, Oakley CE, Edgerton H, Xiong Y, Taheri-Talesh N, Osmani SA, Oakley B. Fusion PCR and gene targeting in Aspergillus nidulans. Nature Protocols 1, 3111 – 3120, 2006 De Souza CP, Osmani SA. Mitosis, not just open or closed. Eukaryotic Cell. 6, 1521-7. Review. 2007 Taheri-Talesh N, Horio T, Araujo-Bazan L, Dou X, Espeso E, Penalva M, Osmani SA, Oakley BR. The Tip Growth Apparatus of Aspergillus nidulans. Mol Biol Cell. In Press. 2008 Ukil L, Varadaraj A, Liu HL and Osman SA. (2008) Copy number suppressors of the Aspergillus nidulans nimA1 mitotic kinase display distinctive and highly dynamic cell cycle regulated locations. Eukaryotic Cell. In Press Liu H-L and Osmani SA. (2008) Keeping it together, linking the main soluble nuclear pore subcomplex functionally with membrane and Ndc1. In Revision. De Souza CPC, Hashmi SB, Nayak T, Oakley BR and Osmani SA. (2008) Mlp1 Associates with Mitotic Kinetochores and Locates Mad1 to a Telophase Structure in Aspergillus nidulans. In Revision. De Souza CPC and Osmani SA. (2008) Mitotic cell cycle control. In, Cellular and Molecular Biology of Filamentous Fungi. Edited by Katherine A. Borkovich and Daniel J. Ebbole. ASM Press. Washington, DC. Under Review. Ukil L, De Souza CPC, Liu H-L and Osmani SA. (2008) Spindle independent mitosis and segregation of the nucleolus. Under Review. Son S and Osmani SA. (2008) Analysis of phosphatase genes in Aspergillus nidulans identifies a role for Fcp1 in mitosis. Under Review. De Souza CPC and Osmani SA (2009) The value of filamentous fungi as model genetic organisms. Genetics. Invited review.

C. Research Support

Ongoing Research Support

09/20/2005-09/19/2009 1) NIH R01 GM 42564-18 ($225,000 per year direct costs) Title: The Role of the nimA Gene in Mitotic Regulation PI: Stephen A. Osmani, Ph.D.

PHS 398 (Rev. 05/01) Page 3 of 3 Biographical Sketch

Principal Investigator/Program Director (Last, first, middle): Park, Hay-Oak

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE

Park, Hay-Oak Associate Professor

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) Seoul National University, Seoul, Korea B.S. 1981 Biochemistry/Nutrition Seoul National University, Seoul, Korea M.S. 1983 Biochemistry University of Wisconsin, Madison, Wisconsin Ph.D. 1991 Mol. Biol./Biochem. University of California, San Francisco, CA Postdoc. 1996 Biochem. & Cell Biol.

A. Positions and Honors. Academic Appointments 1981-1983 Graduate Student, Seoul National University, Supervisor, Dr. Inwon Park 1984-1991 Graduate Student, University of Wisconsin, Madison, Advisor, Dr. Elizabeth A. Craig 1991-1996 Postdoctoral Fellow, University of California, San Francisco, Sponsor, Dr. Ira Herskowitz 1996-2002 Assistant Professor, Department of Molecular Genetics, Ohio State Biochemistry Program, Molecular, Cellular, & Developmental Biology Program, The Ohio State University 2002-present Associate Professor, Department of Molecular Genetics, The Ohio State University

Other Experience and Professional Memberships August 2005, summer course 'Molecular Biology of Aging'; Marine Biological Laboratory, Woods Hole, MA; supported by Ellison Medical Foundation Ad hoc Reviewer of manuscripts for journals (Mol Biol Cell, Nature Cell biology, PNAS, EMBO J, Mol. Microbiology, and BioTechniques). Ad hoc Reviewer of grant proposals for NSF (since 2004) External reviewer for tenure & promotion for University of Virginia (2006) and University of Massachusetts at Boston (2008) External examiner for a PhD Candidacy Exam at University of Illinois - Chicago (2008) Member, American Heart Association Region I Basic Cell Peer Review Committee (2008 - present) Honors and Awards 1986-1989 Fellowship from Lucille P. Markey Charitable Trust, Miami, Florida 1995-1996 Boyer fellowship, University of California-San Francisco 1996-1997 Beginning Investigator Award, American Cancer Society, Ohio division 1998-2000 Basil O’Connor Starter Scholar Research Award, March of Dimes Birth Defect Foundation

B. Publications. (* Corresponding author) Park, H.-O., S. Kang, K. Lee, and I. Park* (1983) Modified Nucleosides in Yeast tRNALeu3 and Ile tRNA AUA Precursors. Korean Biochem J., 16 (4):267-279. Craig, E. A.*, M. R. Slater, D. E. Stone, H.-O. Park, and W. R. Boorstein (1987) Regulation of a Yeast Heat Shock Gene. In RNA Polymerase and the Regulation of Transcription, eds. W. S. Reznikoff, R. R. urgess, J. E. Dahlberg, C. A. Gross, M. T. Record, Jr., and M. Wickens, Elsevier Science Publishing Co., pp. 267-278. Park, H.-O. and E. A. Craig* (1989) Positive and Negative Regulation of Basal Expression of a Yeast HSP70 Gene. Mol. Cell. Biol. 9:2025-2033.

PHS 398/2590 (Rev. 05/01) Page ___8____ Biographical Sketch Format Page Principal Investigator/Program Director (Last, first, middle): Park, Hay-Oak Craig, E. A.*, W. R. Boorstein, H.-O. Park, D. E. Stone, and C. Nicolet (1989) Complex Regulation of three heat inducible HSP70 related genes in Saccharomyces cerevisiae. In Stress-Induced Proteins, UCLA Symp. Mol. Cell. Biol., New Series, Alan Liss, Inc. pp. 51-62. Park, H.-O. and E. A. Craig* (1991) Transcriptional regulation of a yeast HSP70 gene by heat shock factor and an upstream repression site-binding factor. Genes & Dev. 5: 1299-1308. Park, H.-O., J. Chant, and I. Herskowitz* (1993) BUD2 encodes a GTPase activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast. Nature 365: 269-274. Herskowitz, I.*, H.-O. Park, S. Sanders, N. Valtz, and M. Peter (1995) Programming of cell polarity in budding yeast by endogenous and exogenous signals. In Cold Spring Harbor Symp. Quant. Biol., Vol. LX, Cold Spring Harbor Press, pp. 717-727. Park, H.-O.* and J. Chant (1996) Bud2 protein. In The Guidebook to the small GTPases, ed. M. Zerial and L. Huber, Oxford Press, pp. 200-203. Peter, M., A. Neiman, H.-O. Park, M. van Lohuizen, and I. Herskowitz* (1996) Interaction between the small GTP-binding protein Cdc42 and the protein kinase Ste20 is necessary for signal transduction in the yeast pheromone pathway. EMBO J. 15: 7046-7059. Park, H.-O.*, E. Bi, J. Pringle, and I. Herskowitz (1997) Two active forms of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity. Proc. Natl. Acad, Sci. USA 94: 4463- 4468. Park, H.-O.*, A. Sanson, and I. Herskowitz (1999) Localization of Bud2, a GTPase activating protein necessary for programming cell polarity in yeast, to the presumptive bud site. Genes & Dev. 13: 1912- 1917. Kang, P. J., A. Sanson, B. Lee, and H.-O. Park* (2001) A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity. Science 292:1376-1378. (Published online Science Express, 19 April, 2001) - featured in the review by R. Arkowitz, Curr. Biol. (2001) 11:R610-R612 Park, H.-O.*, P. J. Kang, and A. W. Rachfal (2002) Localization of the Bud1/Rsr1 GTPase involved in selection of a proper growth site in yeast. J. Biol. Chem. 277: 26721-26724 Park, H.-O.* and K. G. Kozminski (2003) Yeast small G protein function: molecular basis of cell polarity in yeast, in Signaling Handbook, eds. T. Hunter & H. Hamm, Vol. 2, pp.733-736, Academic Press, San Diego. Kozminski, K. G.#, L. Béven#, E. Angerman, A. H. Y. Tong, C. Boone, and H.-O. Park * (2003) Interaction between a Ras-like and a Rho-like GTPase couples the selection of a growth site to the development of cell polarity. Mol. Biol. Cell 14: 4958-4970 (# These authors equally contributed to the work.) - "MBC Paper of the Year" Award Kang, P. J., B. Lee, and H.-O. Park* (2004) Specific residues of the GDP/GTP exchange factor Bud5p are involved in establishment of the cell-type-specific budding pattern in yeast. J. Biol. Chem. 279: 27980- 27985. Kang, P. J., E. Angerman, K. Nakashima, J. R. Pringle, and H.-O. Park* (2004) Interactions among Rax1p, Rax2p, Bud8p, and Bud9p in Marking Cortical Sites for Bipolar Bud-site Selection in Yeast. Mol. Biol. Cell 15: 5145-5158 Park, H-O.* and E. Bi (2007) Central roles of small GTPases in the development of cell polarity in yeast and beyond. Microbio. Mol. Bio. Reviews 71: 48-96 Singh, K., P. J. Kang, and H.-O. Park* (2008) The Rho5 GTPase is necessary for oxidant-induced cell death in budding yeast. Proc. Natl. Acad. Sci. USA 105: 1522-1527 Kozminski, K. G. and Park, H.-O.* (2008) Yeast small G protein function: molecular basis of cell polarity in yeast, in Signaling Handbook, Second Ed, eds. T. Hunter & H. Hamm, Vol. 2, Academic Press, San Diego. (in press) Kang, P. J., L. Béven, and H.-O. Park* (2008) Polarization of the Cdc42 GTPase mediated by its effectors and its upstream GTPase (submitted)

PHS 398/2590 (Rev. 05/01) Page ___9____ Biographical Sketch Format Page Principal Investigator/Program Director (Last, first, middle): Park, Hay-Oak

C. Research Support (during the last five years)

Current Research Support

• R01 GM076375-02 (PI: H.-O. Park) 1/01/2006 - 12/31/2009 NIH/NIGMS Title: Regulation of polarized cell growth by GTPases The goal of this project is to determine the molecular mechanism by which GTPases regulate polarized cell growth.

• Am Heart Assn-Great Rivers Affiliate, Grant-in-Aid (PI: H.-O. Park) 7/01/07 - 6/30/09 Title: Role of a Rho GTPase in oxidant-induced cell death The goal of this project is to determine the role of a Rho GTPase in oxidant-induced cell death.

Completed Research Support

• R01 GM56997-05 (PI: H.-O. Park) 2/1/99-1/31/04 (extended to 1/31/05) NIH/NIGMS Title: Spatial control of cell polarity during yeast budding The goal of this study is to determine the molecular mechanism by which cell polarity is established.

• Am Cancer Soc-Ohio, LTR DTD (PI: H.-O. Park) 02/01/05 - 07/31/06 Title: Role of a Rho GTPase in oxidant signaling and/or oxidative stress response The goal of this project is to determine the role of a Rho GTPase in oxidative stress response. module regulates polarized cell growth.

• Am Heart Assn-Ohio Affiliate Inc 0555303B (PI: H.-O. Park) 07/01/05 - 06/30/07 (terminated on 12/31/05 upon activation of NIH grant GM076375) Title: A GTPase regulatory circuit involved in cell polarity The goal of this project is to determine the molecular mechanism by which a GTPase module determines cell polarity.

Role of a Rho GTPase in oxidant signaling and/or oxidative stress response

PHS 398/2590 (Rev. 05/01) Page ___10____ Biographical Sketch Format Page BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Randy Scholl Associate Professor, Interim Chair eRA COMMONS USER NAME

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable)

University of Illinois B.S. 1968 Agricultural Science North Carolina State Univ. M.Sc. 1970 Crop Science North Carolina State Univ. Ph.D. 1972 Crop Science

A. Positions and Honors.

Positions and Employment 1968-1972 Research Associate, North Carolina State Univ., Raleigh 1972-1973 Post-doctoral Associate, Univ. of Illinois, Urbana 1972-1978 Assistant Professor, Dept. of Genetics, The Ohio State Univ., Columbus 1978-1987 Associate Professor, Dept. of Genetics, The Ohio State Univ., Columbus 1987-1998 Associate Professor, Dept. of Molecular Genetics, The Ohio State Univ., Columbus 1998-present Associate Professor, Dept. of PCMB, The Ohio State Univ., Columbus

Other Experience and Professional Memberships 1986 Visiting Faculty, DOE Plant Res. Lab., Michigan State Univ., East Lansing 1990-present Director, Arabidopsis Biological Resource Center, Ohio State 2001-2006 Executive Committee, TAIR database 2006-present Interim Chair, Dept. of Plant Cellular & Molecular Biology, Ohio State Univ.

B. Selected peer-reviewed publications (in chronological order).

Scholl, R. L., J. E. Harper and R. H. Hageman. 1974. Improvement of the nitrate color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol. 53:825-828. Scholl, R. L. 1974. The inheritance of isocitratase activity and its relationship to seedling vigor in a cross of upland cotton (Gossypium hirsutum L.). Crop Sci. 14:296-300. Scholl, R. L. 1976. Variability within Gossypium hirsutum L. for seedling isocitratase activity. Crop Sci. 16:710-703. Scholl R. L. and P. A. Miller. 1976. Genetic association between yield and lint quality in cotton. Crop Sci. 16:780-783. Amos, J. A. and R. L. Scholl. 1977. Effect of growth temperature on leaf nitrate reductase, glutamine synthetase, and NADH-glutatamate dehydrogenase of juvenile maize genotypes. Crop Sci. 17: 445-448. Scholl, R. L. and K. M. Dennison. 1978. Sensitivity of cultured tissue of Arabidopsis thaliana races to sulfanilamde. Physiol. Plantar. 43:321-325. Amos, J. A. and R. L. Scholl. 1978. Induction of haploid callus from anthers of four species of Arabidopsis. Z. Ppflanzenphysiol. 90:33-43. Sharma, R. K., B. Griffing and R. L. Scholl. 1979. Variation among Arabidopsis thaliana L. (Heynh.) races for survival in limited carbon dioxide. Theor. Appl. Genet. 54: 11-15. Scholl, R. L. and J. A. Amos. 1980. Isolation of doubled haploid plants through anther culture in Arabidopsis thaliana. Z. Pflanzenphysiol. 96:407-414. Scholl, R. L., D. E. Keathley and T. J. Baribault. 1981. Enhancement of root formation and fertility in shoots regenerated from anther- and seedling-derived callus cultures of Arabidopsis thaliana. Z. Pflanzenphysiol. 104:225-231. Keathley, D. E. and R. L. Scholl. 1982. Culture of Arabidopsis thaliana anthers on liquid medium. Z. Pflanzenphysiol. 106:199-212. Keathley, D. E. and R. L. Scholl. 1983. Chromosomal heterogeneity of Arabidopsis thaliana anther callus, regenerated shoots and plants. Z. Pflanzenphysiol. 112:247-255. Wang, X. -M., R. L. Scholl and K. A. Feldmann. 1986. Characterization of a chlorate-hypersensitive, high nitrate reductase Arabidopsis thaliana mutant. Theor. Appl. Genet. 72:328-336. Zhang, H., R. L. Scholl and C. Somerville. 1988. Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res. 16: 1220. Wang, X., K. A. Feldmann, and R. L. Scholl. 1988. A chlorate-hypersensitive, high nitrate; chlorate uptake mutant of Arabidopsis thaliana. Physiol. Plantar. 73: 305-310. Kim, Y., H. Zhang and R.L. Scholl. 1990. Two evolutionarily divergent genes encode a cytoplasmic ribosomal protein of Arabidopsis thaliana. Gene 92:177-182. You T. H., and Scholl, R. L. 1998. PCR amplification of cDNA Libraries forCloning and Screening. BioTechniques 24:574-575. Garcia-Hernandez M, Berardini TZ, Chen G, Crist D, Doyle A, Huala E, Knee E, Lambrecht M, Miller N, Mueller LA, Mundodi S, Reiser L, Rhee SY, Scholl R, Tacklind J, Weems DC, Wu Y, Xu I, Yoo D, Yoon J, Zhang P. 2002. TAIR: a resource for integrated Arabidopsis data. Funct Integr Genomics. Nov; 2(6): 239-53. Griffing, B. and R.L. Scholl. l991. Qualitative and quantitative studies of Arabidopsis thaliana. Genetics 129:605-609. Scholl, R. L, Ware D. H. and May, S. 2000. Seed and Molecular Resources for Arabidopsis. Plant Physiol. 2000 124: 1477-1480. Meinke, D. and Scholl. R. 2003. The Preservation of Plant Genetic Resources. Experiences with Arabidopsis. Plant Physiol. 133: 1046-1050. Rivero-Lepinckas, Luz, Crist, Deborah, Scholl, Randy 2006. Growth of plants and preservation of seeds. METHODS IN MOLECULAR BIOLOGY 323:3-12.

C. Research Support

Ongoing Research Support

DBI-0542034 Scholl (PI) 5/01/06-3/31/2011 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($2,940,000) Role: PI

Completed Research Support

DBI-0091471-suppl. Scholl (PI) 8/2003-3/2006 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($240,000) Role: PI

DBI-0091471 Scholl (PI) 4/2001-3/2006 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($2,790,000) Role: PI

IBN-9600964-suppl. Scholl (PI) 2/2000-2/2001 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($50,000) Role: PI

IBN-9600964 Scholl (PI) 3/1997-2/2001 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($1,856,000) Role: PI

BIR-9113224 Scholl (PI) 9/1996-1/1997 NSF - extension The Arabidopsis Biological Resource Center at The Ohio State University ($179,000) Role: PI

BIR-9113224 Scholl (PI) 9/1991-8/1996 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($1,940,000) Role: PI

? Scholl (PI) 1979-82 NSF Anther Culture in Arabidopsis ($58,000)

Principal Investigator/Program Director (Last, first, middle):

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Mark A. Seeger Associate Professor

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) Bowling Green State University, Ohio B.S. 1981 Biology Bowling Green State University, Ohio M.S. 1984 Biology Indiana University, Indiana Ph.D. 1989 Dev. Genetics University of California at Berkeley Postdoc 1989-1992 Dev. Neurobiology

NOTE: The Biographical Sketch may not exceed four pages. Items A and B may not exceed two of the four-page limit. A. Positions and Honors. Positions 1989-1992 Jane Coffin Childs Postdoctoral Fellow, University of California, Berkeley, CA. "Cell recognition during neuronal development in Drosophila." Field: Developmental Neurobiology, Advisor: Dr. C.S. Goodman,

1992-1997 Assistant Member, Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK

1997-2002 Assistant Professor, Department of Molecular Genetics and the Neurobiotechnology Center, The Ohio State University, Columbus, OH

2002 - present Associate Professor, Department of Molecular Genetics and the Center for Molecular Neurobiology, The Ohio State University, Columbus, OH Honors 1985-1988 NIH Genetics Training Grant Predoctoral Fellowship, Indiana University 1988 Young Investigator Award, Developmental Biology Society 1989-1992 Postdoctoral Fellow of the Jane Coffin Childs Fund for Medical Research 1992 NIH Genetics Training Grant Postdoctoral Fellowship, University of California, Berkeley 1994 Merrick Young Investigator Award, Oklahoma Medical Research Foundation

B. Selected peer-reviewed publications (in chronological order). 1. Woodruff, R.C., Thompson, J.N., Seeger, M.A., and Spivey, W.E. 1984. Variation in spontaneous mutation and repair in natural population lines of Drosophila melanogaster. Heredity 53: 223-234. 2. Woodruff, R.C., Seeger, M.A., and Norris, E.S. 1987. Clusters of premeiotic ring-X chromosome loss are not observed in males of Drosophila melanogaster. Mutation Research 191: 145-149. 3. Seeger, M.A. and Kaufman, T.C. 1987. Homoeotic genes of the Antennapedia Complex (ANT-C) and their molecular variability in the phylogeny of the Drosophilidae. In Raff, R.A. and Raff, E.C. (eds.): "Development as an Evolutionary Process." New York: Alan R. Liss, Inc., pp. 179-202. 4. Seeger, M.A., Haffley, L. and Kaufman, T.C. 1988. Characterization of amalgam: a member of the immunoglobulin superfamily from Drosophila. Cell 55: 589-600.

PHS 398/2590 (Rev. 05/01) Page _____ Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b. Principal Investigator/Program Director (Last, first, middle):

5. Seeger, M.A. and Kaufman, T.C. 1990. Molecular analysis of the bicoid gene from Drosophila pseudoobscura: identification of conserved domains within coding and noncoding regions of the bicoid mRNA. EMBO J. 9: 2977-2987. 6. Kaufman, T.C., Seeger, M.A. and Olsen, G. 1990. Molecular and genetic organization of the Antennapedia gene complex of Drosophila melanogaster. Adv. in Genetics 27: 309-362. 7. Woodruff, R.C. and Seeger, M.A. 1991. Test of a semiselective screen for induced aneuploidy in germ cells of Drosophila melanogaster females with structurally normal chromosomes. Mutation Research 252: 61-82. 8. Randazzo, F.M., Seeger, M.A., Huss, C.A., Sweeney, M.A., Cecil, J.K., and Kaufman, T.C. 1993. Structural changes in the Antennapedia Complex of Drosophila pseudoobscura. Genetics 133: 319-330. 9. Seeger, M.A., Tear, G., Ferres-Marco, D., and Goodman, C.S. 1993. Mutations affecting growth cone guidance in Drosophila: Genes necessary for guidance towards or away from the midline. Neuron 10: 409- 426. 10. Tear, G., Seeger, M.A. and Goodman, C.S. 1993. To cross or not to cross: a genetic analysis of guidance at the midline. Perspectives on Developmental Neurobiology 1:183-194. 11. Seeger, M.A. 1994. Genetic and molecular dissection of axon pathfinding in the Drosophila nervous system. Curr. Opin. Neurobiol. 4: 56-62. 12. Tear, G., Harris, R., Sutaria, S., Kilomanski, K., Goodman, C.S., and Seeger, M.A. 1996. commissureless controls growth cone guidance across the CNS midline in Drosophila and encodes a novel membrane protein. Neuron 16: 501-514. 13. Harris, R., Sabatelli, L.M. and Seeger, M.A. 1996. Guidance cues at the Drosophila CNS midline: Identification and characterization of two Drosophila Netrin/UNC-6 homologues. Neuron 17: 217-228. 14. Wolf, B., Seeger, M.A., and Chiba, A. 1998. Commissureless endocytosis is correlated with initiation of neuromuscular synaptogenesis. Development 125: 3853-3863. 15. Gong, Q., Rangarajan, R., Seeger, M., and Gaul, U. 1999. The Netrin receptor Frazzled is required in the target for establishment of retinal projections in the Drosophila visual system. Development 126: 1451-1456. 16. Seeger, M.A. and Beattie, C.E. 1999. Attraction versus repulsion: Modular receptors make the difference in axon guidance. Cell 97: 821-824. 17. Liebl, E., Forsthoefel, D.J., Franco, L.S., Sample, S.H., Hess, J.E., Cowger, J.A., Chandler, M.P., Jackson, A,M., and Seeger, M.A. 2000. Dosage-sensitive, reciprocal genetic interactions between the Abl tyrosine kinase and the putative GEFTrio reveal a role in axon pathfinding. Neuron 26: 107-118. 18. Fremion, F., Darboux, I., Diano, M., Hipeau-Jacquotte, R., Seeger, M.A., and Piovant, M. 2000. Amalgam is a ligand for the transmembrane receptor Neurotactin and is required for Neurotactin mediated cell adhesion and axon fasciculation in Drosophila. EMBO J. 19: 4463-4472. 19. Challa A.K., Beattie, C.E. and Seeger, M.A. 2001. Identification and characterization of roundabout orthologs in zebrafish. Mechanisms of Development 101: 249-253. 20. Zhao, C., York, A., Yang, F., Forsthoefel, D.J., Dave, V., Fu, D., Zhang, D., Corado, M.S., Small, S., Seeger, M.A., and Ma, J. 2002. The activity of the Drosophila morphogenetic protein Bicoid is inhibited by a domain located outside its homeodomain. Development 129:1669-80. 21. Liebl, E.C., Rowe, R.G., Forsthoefel, D.J., Stammler, A.L., Schmidt, E.R., Turski, M., and Seeger, M.A. 2003. Interactions between the secreted protein Amalgam, its transmembrane receptor Neurotactin and the Abelson tyrosine kinase affect axon pathfinding. Development 130: 3217-3266. 22. McGovern, V.L., Pacak, C.A., Sewell, S.T., Turski, M.L., and Seeger, M.A. 2003. A targeted gain of function screen in the embryonic CNS of Drosophila. Mechanisms of Development.120: 1193-1207. 23. McGovern, V.L. and Seeger, M.A. 2003. Mosaic analysis reveals a cell-autonomous, neuronal requirement for commissureless in the Drosophila CNS. Development, Genes & Evolution 213: 500-504. 24. Forsthoefel, D.J., Liebl, E.C., Kolodziej, P.A., and Seeger, M.A. 2005. The Abelson tyrosine kinase, the Trio GEF, and Enabled interact with the Netrin receptor Frazzled in Drosophila. Development 132: 1983-1994. 25. Challa, A.K., McWhorter, M.L., Wang, C., Seeger, M.A., Beattie, C.B. 2005. Robo3 isoforms have distinct roles during zebrafish development. Mechanisms of Development 122: 1073-1086.

C. Current funding

National Science Foundation IOS-0821103 “Commissureless Regulation of Robo-Slit Signaling in Insects” 9/1/2008 to 8/31/2010, $125,000 total costs per year

PHS 398/2590 (Rev. 05/01) Page _____ Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b. Principal Investigator/Program Director (Last, first, middle): Simcox, Amanda, A

BIOGRAPHICAL SKETCH GoodnigJ Provide the following information for the key personnel in the order listed for Form Page 2. Comment: For any page on which you wish Follow the sample format for each person. DO NOT EXCEED FOUR PAGES. to edit a Header or Footer, select “Unprotect Document” under the Tools menu, double- NAME POSITION TITLE click in the Header/Footer, and enter text. Re- Amanda Simcox Professor protect forms: Select “Protect Document for FORMS” under the Tools menu. EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) University of Sussex, UK B. Sc. 1976 Biology University of Sussex, UK Ph. D. 1981 Genetics The Johns Hopkins University Post-doctoral 1981-1984 Insect tissue-culture

A. Positions and Honors.

Positions and Professional Experience

1984-1988 Associate Research Scientist, The Johns Hopkins University Baltimore, MD 1988-1989 Lecturer, University of Sussex Falmer, Brighton, UK 1989-1990 Associate Research Scientist, The Johns Hopkins University, Baltimore, MD 1990-1997 Assistant Professor, Molecular Genetics, The Ohio State University, Columbus, OH 1997-2003 Associate Professor, Molecular Genetics, The Ohio State University, Columbus, OH 2000-2001 Sabbatical Visitor, University of Sussex, Falmer, Brighton, UK 2003-present Professor, Molecular Genetics, The Ohio State University, Columbus, OH

Professional Offices

1996- 1999 US ARMY Breast Cancer Research Program: Molecular Genetics panel member 1999-present Member Drosophila Stock Center Board 2001-present National Science Foundation: Developmental Mechanisms panel member 2003 Sandler Memorial Lecture Selection Committee, chair 2005-present Member National Drosophila Board 2006 Member PO1 and Postdoctoral Felowship NIH study Sections

Honors

2000-2001 Fogarty International Center (NIH), Senior Fellowship 2002 Faculty Service Award (College of Arts and Sciences Ohio State University) Simcox, Amanda, A B. Peer-reviewed publications (since joining OSU in 1990; joint senior author indicated by *)

Simcox, A. A., Hersperger, E., Shearn, A., Whittle, J.R.S. and Cohen, S. Establishment of imaginal discs and histoblast nests in Drosophila. (1991). Mech. Dev. 34, 11-20. Cohen, B., Simcox, A.A., and Cohen, S.M. (1993). Allocation of the thoracic imaginal primordia in the Drosophila embryo. Development 117, 597-608. *Tohtong, R., Yamashita, H., Graham, M., Haeberle, J., Simcox, A., and Maughan, D. (1995). Impairment of flight ability and flight muscle function caused by mutations of phosphorylation sites of myosin regulatory light chain in Drosophila melanogaster. Nature 374, 650-653. Accompanied by a News and Views article. Simcox, A. A., Grumbling, G., Schnepp, B., Bennington, C., Hersperger, E. and Shearn, A. (1996). Molecular, phenotypic, and expression analysis of vein, a gene required for growth of the Drosophila wing disc. Dev. Biol. 177, 475-489.

PHS 398/2590 (Rev. 05/01) Page ______Biographical Sketch Format Page ! Principal Investigator/Program Director (Last, first, middle): *Kreuz, A., Simcox, A., and Maughan, D. (1996). Alterations in flight muscle ultrastructure and function in Drosophila tropomyosin mutants. J. Cell Biol. 135, 673-687. Schnepp, B., Grumbling, G., Donaldson, T., and Simcox, A. (1996). Vein is a novel component in the Drosophila epidermal growth factor receptor pathway with similarity to the neuregulins. Genes & Dev. 10, 2302-2313. Tohtong, R., Rodriguez, D., Maughan, D. and Simcox, A. (1997). Analysis of cDNAs encoding Drosophila myosin light chain kinase. J. Mus. Res. Cell Mot. 18, 43-56. Simcox, A. Differential requirement for EGF-like ligands in Drosophila wing development. (1997). Mech. Dev. 62, 41-50. Dickinson, M. H., Hyatt, C. J., Lehmann, F.-O., Moore, J., Reedy, M., Simcox, A., Tohtong, R., Vigoreaux, J. O., Yamishita, H., and Maughan, D. (1997). Phosphorylation-dependent power output of transgenic flies: an integrated study. Biophys., J. 73, 3122-3134. Schnepp, B., Donaldson, T., Grumbling, G., Ostrowski, S., Schweitzer, R., Shilo, B-Z. and Simcox, A. (1998). EGF domain swap converts a Drosophila EGF-receptor activator into an inhibitor. Genes & Dev. 12, 908- 913. Wessells, R. J., Grumbling, G., Donaldson, T., Wang, S-H., and Simcox, A. (1999). Tissue-specific regulation of Vein/EGF-receptor signaling in Drosophila. Dev. Biol. 216, 243-259. Martin JF, Hersperger E, Simcox A, Shearn A. (2000) minidiscs encodes a putative amino acid transporter subunit required non-autonomously for imaginal cell proliferation. Mech. Dev. 92, 155-167. Wang, S-H, Simcox, A.* and Campbell, G. (2000). Dual role for EGF-receptor signaling in early wing disc development. Genes & Dev. 14, 2271-2276. Irving, T., Bhattacharya, S., Tesic, I., Simcox, A., Vigoreaux, J., Maughan, D. (2001). Changes in myofibrillar structure and function produced by N-terminal deletion of the regulatory light chain in Drosophila. J Muscle Res Cell Motil. 22, 675-83. Butler, M., Jacobsen, T., Cain, D.M., Jarman, M., Hubank, M., Whittle, J.R.S., Phillips, R. and Simcox, A. (2003). Identification of genes differentially expressed in the Drosophila wing disc using high-density DNA oligonucleotide arrays. Development 130, 659-670. Donaldson, T., Wang, S-H., Jacobsen, T.L., Schnepp, B., Price, J., and Simcox, A. (2004) Regulation of the Drosophila EGF-ligand Vein is mediated by multiple domains. Genetics 167, 687-98. Jacobsen, T..J., Cain, D., Paul, L., Justiniano, S., Alli, A., Mullins, J., Wang, CP., Butchar, J.P. and Simcox, A. (2006). Functional analysis of genes differentially expressed in the Drosophila wing disc: Role of transcripts enriched in the wing region. Genetics 174, 1973-1982. Simcox, A., Mitra, S., Truesdell, S., Paul, L., Chen, T., Butchar, J.P., Justiniano, S. (2008) Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes. PLoS Genetics 4:8 e1000142. Simcox, A., Jafar-Nejad, H. (2009) Drosophila embryonic fibroblasts: extending mutant analysis in vitro. Fly ‘extra view’ invited article.

C. Research Support

NIH-RO1 Simcox (PI) 2004-2009 ($1,198,480 total costs) NIH Development of tissue-specific cell lines in Drosophila This study is to devise genetic methods to establish cell lines in Drosophila. The role of conserved oncogenes and tumor suppressors will be investigated

DBI-0453485 Simcox (PI, Gopalan, Co-PI)) 2005-2010 ($451,705 total costs) NSF REU Site: Undergraduate Research in Molecular Genetics. This a summer research program for undergraduates, primarily from groups underrepresented in science.

+ PHS 398/2590 (Rev. 05/01) Page ______Biographical Sketch Format Page + BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE David E. Somers Associate Professor eRA COMMONS USER NAME

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Ohio University, Athens, OH B.Sc. 1973-1977 Botany Physiological Plant University of Alberta, Edmonton, Alberta Canada. M.Sc 1977-1981 Ecology Ph.D. 1987-1994 Plant Biology University of California at Berkeley, Berkeley

Post- The Scripps Research Institute, La Jolla, CA 1994-1999 Plant Mol. Genetics doctoral

A. Positions and Honors. Positions and Employment 1977-1980 Graduate Teaching Assistant. Dept. of Botany, University of Alberta, Edmonton, Alberta, Canada. 1981-1982 Research Assistant, Dept. of Botany, University of Washington, Seattle, WA. 1985-1987 Research Technician, Howard Hughes Medical Institute. University of Washington, Seattle, WA. 1987-1994 Graduate Research Assistant, Dept. of Plant Biology, UC-Berkeley, Berkeley, CA. Peter Quail, advisor. 1994-1999 Postdoctoral Research Fellow, Dept. of Cell Biology, The Scripps Research Institute, La Jolla, CA. Steve A. Kay, advisor. 1999-2005 Assistant Professor, Department of Plant Biology, The Ohio State University, Columbus OH 2005-present Associate Professor, Department of Plant Biology, The Ohio State University, Columbus OH Other Experience and Professional Memberships Member: Society for Research on Biological Rhythms Member: American Society of Plant Biologists Grant review panels 2005 National Science Foundation, Integrative Plant Biology Panel 2005-2006 National Institute of Health, CSD Study Section 2006 National Institute of Health, NRSA Cell Biology and Development (F05) Study Section 2007-2008 National Institute of Health, Neurogenesis and Cell Fate Study Section, Cellular Signaling and Regulatory Systems Ad hoc reviews 2000-present NSF, USDA, BBSRC (UK), BSF (US-Israel Binational Science Foundation), Marsden Fund (The Royal Society of New Zealand) Journal Peer review Science, Nature, PNAS, Current Biology, Nature Reviews Microbiology, PLoS Biology, The Plant Cell, Plant Journal, Plant Physiology, Plant Molecular Biology, BMC Plant Biology , Planta, Cell Research, Journal of Biological Rhythms, Plant and Cell Physiology, Philos Trans R Soc Lond B Biol Sci., Plant Cell and Environment, Photochemistry and Photobiology, Journal of Phycology Honors 1997 JSPS Short-Term Invitation Fellow, Hitachi Advanced Research Lab, Saitama, Japan. 1994-1997 NSF Postdoctoral Research Fellow in Plant Biology B. Selected peer-reviewed publications (in chronological order; selected from 30 peer- reviewed publications). 1. Somers, D.E., Sharrock, R.A., Tepperman, J.T., and Quail, P.H. 1991. The hy3 long 1 hypocotyl mutant of Arabidopsis is deficient in phytochrome B. Plant Cell 3:1263-1274. 2. Devlin, P. F., Rood, S., Somers, D.E., Quail, P.H., and Whitelam, G.C. 1992. Photophysiology of the elongated internode (ein) mutant of Brassica rapa: The ein mutant lacks a detectable phytochrome B-like polypeptide. Plant Physiol 100:1442-1447. 3. Wester, L., Somers, D.E., Clack, T. and Sharrock, R.A. 1993. Transgenic complementation of the hy3 phytochrome B mutation and PHYB gene copy number effects in Arabidopsis. Plant J. 5:261-272 4. Dehesh, K., Franci, C., Sharrock, R.A., Somers, D.E., Welsh J. and Quail, P.H. 1994. The Arabidopsis phytochrome A gene has multiple transcription start sites and a promoter sequence motif homologous to the repressor element of monocot phytochrome A genes. Photochem. Photobiol. 59: 379-384. 5. Somers, D.E. and Quail, P.H. 1995. Phytochrome-mediated light regulation of PHYA- and PHYB-GUS transgenes in Arabidopsis thaliana seedlings. Plant Physiol 107:523-534. 6. Somers, D.E. and Quail, P.H. 1995. Temporal and spatial expression patterns of PHYA and PHYB genes in Arabidopsis. Plant J. 7:413-427 7. Hicks, K.A., Millar, A.J., Carre, I.A., Somers, D.E., Straume, M., Meeks-Wagner, D.R. and Kay, S.A. 1996. Conditional circadian dysfunction of the Arabidopsis early-flowering 3 mutant. Science 274: 790-792. 8. Anderson, S.L., Somers, D.E., Millar, A.J., Hanson, K., Chory, J. and Kay, S.A. 1997. Attenuation of phytochrome A and B signaling pathways by the Arabidopsis circadian clock. Plant Cell 9:1727-1743. 9. Devlin, P.F., Somers, D.E., Quail, P.H., and Whitelam, G.C. 1997. The Brassica rapa elongated internode (EIN) gene encodes phytochrome B. Plant Mol. Biol. 34:537-547. 10. Somers, D.E., Devlin, P.A. and Kay, S.A. 1998. Phytochromes and cryptochromes in the entrainment of the Arabidopsis circadian clock. Science 282:1488-1490. 11. Somers, D.E., Webb, A., Pearson, M., and Kay, S.A. 1998. The short-period mutant, toc1-1, alters circadian clock regulation of multiple outputs throughout development in Arabidopsis thaliana. Development 125:485-494. 12. Park D.H.*, Somers D.E.*, Kim Y.S., Choy Y.H., Lim H.K., Soh M.S., Kim H.J., Kay S.A., Nam H.G. 1999. Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science 285: 1579-1582. *(equal contributions as first authors). 13. Somers, D.E., Schultz, T.F., Milnamow, M. and Kay S.A. 2000. ZEITLUPE encodes a novel clock associated PAS protein from Arabidopsis. Cell 101:319-329. 14. Strayer, C., Oyama, T., Schultz, T.F., Raman, R., Somers, D.E., Mas, P., Panda, S., Kreps, J.A.and Kay, S.A. 2000. Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog. Science 289:768-771. 15. Kim, W-Y.*, Geng, R.* and Somers, D.E. 2003. Circadian phase-specific degradation of the F- box protein ZTL is mediated by the proteasome. Proc Natl Acad Sci U S A 100:4933-4938. *(equal contributions as first authors). 16. Risseeuw, E.P., Daskalchuk, T.E., Banks, T.W., Liu, E., Cotelesage, J., Hellman, H., Estelle, M., Somers, D.E., and Crosby, W.L. 2003. Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis. Plant J. 34:753-767. 17. Mas, P., Kim, W-Y., Somers D.E., and Kay S.A. 2003. Targeted degradation of TOC1 by ZTL modulates circadian function in Arabidopsis thaliana. Nature 426:567-70. 18. Somers, D.E., Kim, W-Y., Geng, R. 2004. Dosage-dependent control of circadian period and photomorphogenesis by the F-box protein ZEITLUPE. Plant Cell 16:769-782. 19. Han, L., Mason, M, Risseeuw, E.P., Crosby, W.L. and Somers, D.E. 2004. Formation of an SCFZTL complex is required for proper regulation of circadian timing. Plant J. 40:291-301. 20. Kim, W.Y., Hicks, K. A. and Somers, D.E. 2005. Independent roles for EARLY FLOWERING 3 and ZEITLUPE in the control of circadian timing, hypocotyl length, and flowering time. Plant Physiol. 139: 1557-69. 21. Kevei, E., Gyula, P., Hall, A., Kozma-Bognar, L., Kim, W.Y., Eriksson, M.E., Toth, R., Hanano, S., Feher, B., Southern, M.M., Bastow, R.M., Viczian, A., Hibberd, V., Davis, S.J., Somers, D.E., Nagy, F., and Millar, A.J. 2006. Forward genetic analysis of the circadian clock separates the multiple functions of ZEITLUPE. Plant Physiol. 140:933-945. 22. Allen T., Koustenis A., Theodorou G., Somers, D.E., Kay S.A., Whitelam G.C., Devlin P.F. 2006. Arabidopsis FHY3 specifically gates phytochrome signaling to the circadian

2 clock. Plant Cell 18:2506-2516 23. Kim,W.Y.*, Fujiwara, S.*, Suh, S.S., Kim, J., Kim, Y., Han, L., David, K., Putterill, J., Nam, H.G., and Somers, D.E. 2007. ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light. Nature 449:356-360. *(equal contributions as first authors). 24. Jin JB, Jin YH, Lee J, Miura K, Yoo CY, Kim WY, Van Oosten M, Hyun Y, Somers DE, Lee I, Yun DJ, Bressan RA, Hasegawa PM. 2008. The SUMO E3 ligase, AtSIZ1, regulates flowering by controlling a salicylic acid-mediated floral promotion pathway and through effects on FLC chromatin structure. Plant J. 3:530-40 25. Fujiwara, S.*, Wang, L.*, Han, L., Suh, S. S., Salome, P. A., McClung, C. R., Somers, D.E. 2008. Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo- response regulator proteins. J. Biol. Chem. 283: 23073-23083. *(equal contributions as first authors). 26. Somers, D. E. and Fujiwara, S. 2009. Thinking outside the F-box: novel ligands for novel receptors. Trends Plant Sci. (in submission).

C. Research Support Ongoing Research Support NSF Molecular and Cellular Biosciences 05/01/2006 - 04/30/2009 MCB-0544137 Somers (PI) Functional Domains and Spatial-Temporal Interactions of an Arabidopsis Circadian Clock-Associated F-Box Protein”. This study focuses on the functional dissection of ZTL, its intracellular site of action, and identifies additional biochemical interactors.

NSF Integrative Organismal Biology 02/01/2008 - 01/31/2011 IOS-0748749 Somers (PI) The control of reproductive onset and the circadian clock by GIGANTEA. This project defines the biochemical function of GI by identifying which domains are effective for the interaction and stabilization of ZTL and identifies other GI interactors.

Completed Research Support NSF Molecular and Cellular Biosciences 04/01/2001 - 03/31/2004 MCB-0080090 Somers (PI) Molecular and genetic analysis of ZTL function in the Arabidopsis circadian clock”. This work positioned the ZTL locus within the context of the known red light and blue light signal transduction pathways and identified physical interaction partners of the ZTL protein.

CSREES/USDA Plant Growth and Development 09/01/2002 - 08/31/2004 2002-35304-12594 Somers (PI) Functional in planta analyses of a clock-associated F-box protein. The goals of this project was to identify and confirm the functional domains of ZTL.

NSF Integrative and Organismal Biology: 04/01/2004 - 03/31/2007 IBN-0344377 Somers (PI) Genetic and molecular interactors in circadian clock function. The major goals of this project were to identify genetic suppressors and enhancers of the circadian clock component ZEITLUPE.

NSF Biological Infrastructure 05/01/2005 - 04/30/2008 DBI-0500601 Somers (lead PI) High-resolution CCD camera and luminometer for high-throughput luciferase-based promoter analysis. This award supported the purchase of two systems used to localize and measure luciferase activity resulting from expression of chimeric reporter genes that consist of the luciferase gene fused to a promoter region of the genome. One system is a high throughput luminometer and the other is based on a low-light CCD camera.

3 Principal Investigator/Program Director (Last, first, middle):

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES. NAME POSITION TITLE Vaessin, Harold, E.F., Ph.D. Professor EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY University of Freiburg, Germany Staatsexamen 1976 -1982 Biology & Chemistry University of Cologne, Germany Dr.rer.nat 1983 - 1986 Developmental Physiology University of Cologne, Germany Post Doc 1986 - 1987 Neural Developmental U.C.S.F., San Francisco, CA Post Doc 1987 - 1990 Neural Developmental U.C.S.F., San Francisco, CA Assist.Res. 1990 - 1991 Neural Developmental Physiol. A. Positions and Honors Academic Appointments 1986-1987 Postdoctoral Associate, University of Cologne, Germany 1987-1990 Postdoctoral Associate, Howard Hughes Medical Institute, UCSF, San Francisco, CA 1990-1991 Assistant Research Physiologist, Department of Physiology & Howard Hughes Medical Institute, UCSF, San Francisco, CA 1992-1997 Assistant Professor, Department of Molecular Genetics, Neurobiotechnology Center, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 1997-2004 Associate Professor, Department of Molecular Genetics, Center for Molecular Neurobiology, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 2000-2001 Program Director (IPA), NSF/BIO/IBN, Developmental Neuroscience Program, National Science Foundation, Arlington, VA 2004 –present Professor, Department of Molecular Genetics, Center for Molecular Neurobiology Member, Comprehensive Cancer Center, The Ohio State University, Columbus, OH Honors and Awards Member of OSU Presidential Search Committee; search of new President of The Ohio State University; (2006 - 2007). American Cancer Society 2001 Heroes of Hope Award (together withDrs. Bloomfield, Anderson, Caligiuri, Lee, Mansky, Parthun, Timmers, Wells and Weinstein). Alfred P. Sloan Fellow (Neuroscience) 1993-1997; Alfred P. Sloan Foundation. Postdoctoral Fellowship from Deutsche Forschungsgemeinschaft (DFG); Germany, 1987-1989. Doctorate,with distinction, in Developmental Physiology, 1986, Institute for Developmental Physiology; University of Cologne, Germany Service & Committee activities: Member, ad hoc Committee on Semesters (2008- present) Member, CCI (2008 – 2011) Chair, CCI Assessment subcommittee (2008 –present) Member GEC Advisory committee; (Chair; starting Winter quarter 2009) Member, University Senate Fiscal Committee (9/2007- 8/2010) Chair, University Senate Steering Committee (6/2006 – 6/2007) Chair, Executive Committee of University Senate (6/2006 – 6/2007) Presidential Search Committee member (10/2006 - 7/2007) Senator; University Senate 2004 to 2007; 2008 - 2011) Member, Steering Committee, University Senate, 2005 – 2007 Council on Academic Affairs; 10/2004 to 9/2006; Council on Academic Affairs; SubCommA. A 10/2004 to 9/2006 (Chair, 9/2005 to 9/2006). Committee for Academic Misconduct; 2004 Science Foundation, IBN; Expert Consultant to IBN; Developmental Neuroscience Program, Chair of Developmental Neuroscience Advisory Panel, National Science Foundation; September 2003 to November 2003

PHS 398 (Rev. 05/01) Page 1 Biographical Sketch Enter Grant Name Here Principal Investigator/Program Director (Last, first, middle):

Consultant to IBN; Developmental Neuroscience Program, Chair of Developmental Neuroscience Advisory Panel, National Science Foundation; 9/22/01 to 9/29/01 Program Director, Developmental Neuroscience Program, National Science Foundation, IBN; 9/5/2000 to 9/4/2001; Faculty Advisory Committee to the Dean (Biological Sciences) (1998 – 2000 and 2001 – 2003, Chair 2002 to 2003) University Graduate School Fellowship Selection Committee Member; (1999-2000) MCDB Graduate Committee (1999- 2000 and 2001 to present) Molecular Genetics Graduate Committee (1994-1998) Molecular Genetics Promotion and Tenure Committee (1997-present) Faculty Search Committee for Molecular Neurobiology Committee; MLS Initiative (1995 -1996)Molecular Genetics Faculty Search Committees (1994 –1998, 2002-2003, 2007-2008) Entomology Faculty Search Committee ( 2007-2008)

Selected Publications (total number: 34) Vaessin H, Bremer KA, Knust E, Campos-Ortega JA. The neurogenic gene Delta of Drosophila melanogaster is expressed in neurogenic territories and encodes a putative transmembrane protein with EGF-like repeats. EMBO J, 6:3431-3440, 1987. Caudy* M, Vaessin H*, Brand M, Tuma R, Jan LY, Jan YN. daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex. Cell, 55:1061- 1067, 1988.*first two authors contributed equally Murre C, McCaw PS, Vaessin H, Caudy M, Jan LY, Jan YN, Cabrera CV, Buskin JN, Hauschka SD, Lassar AB, Weintraub H, Baltimore D. Interactions between heterologous Helix-Loop-Helix proteins generate complexes that bind specifically to a common DNA sequence. Cell, 58:537-544, 1989. Bier E, Vaessin H, Shepherd S, Lee K, McCall K, Barbel S, Ackermann L, Carretto R, Uemura T, Grell E, Jan LY, Jan YN. Searching for pattern and mutation in the Drosophila genome with a P-lacZ Vector. Genes & Dev, 3:1273-1287, 1989. Vaessin H, Caudy M, Bier E, Jan LY, Jan YN. The role of Helix-Loop-Helix proteins in Drosophila neurogenesis. Cold Spring Harbor Symposia on Quantitative Biology, 55:The Brain. pp 239-245, 1990. Rao Y, Vaessin H, Jan LY, Jan YN. Neuroectoderm in Drosophila embryos does not require mesodermal induction but depends on the mesoderm for positioning. Genes & Dev, 5:1577-1588, 1991. Vaessin H, Grell E, Wolff E, Bier E, Jan LY, Jan YN. prospero is expressed in neuronal precursors and encodes a nuclear protein which is involved in the control of axonal outgrowth in Drosophila. Cell, 67:941-953, 1991. Younger-Shepherd S, Vaessin H, Bier E, Jan LY, and. JanYN. deadpan, an essential pan-neural gene encoding an HLH protein, acts as a denominator in Drosophila sex determination. Cell, 70:911-922, 1992. Bier E, Vaessin H, Shepherd S, Emery J, Jan LY, Jan YN. deadpan, an essential pan-neural gene in Drosophila encodes a helix-loop-helix protein similar to the hairy gene product. Genes & Dev, 6:2137-2151, 1992. Vaessin H, Brand M, Jan LY, Jan YN. daughterless is essential for neuronal precursor differentiation but not for initiation of neuronal precursor formation in Drosophila embryo. Development, 120:935 – 945, 1994. Feger G, Vaessin H, Wolff E, Su TT, Jan LY Jan YN. dpa, a member of the MCM family, is required for mitotic DNA replication but not endoreplication in Drosophila. EMBO J, 14:5387-5398, 1995. Roark M, Sturtevant MA, Emery J, Vaessin H, Grell E, Bier E. scratch, a pan-neural gene encoding a zinc-finger protein related to snail, promotes neuronal development. Genes&Dev, 9:2384-2398, 1995. Young AP, Murad F, Vaessin H, Xie J, Rife TK. Transcription of the human neuronal nitric oxide synthase gene in the central nervous system is mediated by multiple promotors. In: Nitric oxide: Biochemistry, Molecular Biology and Therapeutic Implications. Ed.: L. Ignarro and F. Murad; Advances in Pharmacology, Academic Press, San Diego. 34:91-112, 1995. Hassan B, and Vaessin H. Regulatory Interactions during early neurogenesis in Drosophila. DevGenet, 18:18-27, 1996. Lane ME, Sauer K, Wallace K, Jan YN, Lehner CF, Vaessin H. Dacapo, a cyclin-dependent kinase inhibitor, stops cell proliferation during Drosophila development. Cell, 87:1225-1235, 1996 Pinsonneault J, Florence B, Vaessin H, McGinnis W. model for extradenticle function as a switch that changes HOX proteins from repressor to activators. EMBO J, 16:2032-2042, 1997. Hassan B, Vaessin H. Daughterless is required for the transcriptional regulation of cell cycle genes in the peripheral nervous system of Drosophila embryos. DevGenetics, 21:117-122, 1997. Hassan B, Li L, Bremer KA, Chang W, Pinsonneault J, Vaessin H. Prospero is a pan-neural transcription factor that modulates homeodomain protein activity. Prod Natl Acad Sci, 94:10991-10996, 1997. Robinson KO, Ferguson HF, Cobey S, Vaessin H, Smith HB. Sperm-mediated transformation of the honey bee, Apis mellifera. Insect Molecular Biology, 9:625-34, 2000.

PHS 398 (Rev. 05/01) Page 2 Biographical Sketch Enter Grant Name Here Principal Investigator/Program Director (Last, first, middle):

Wallace K, Liu T-H, Vaessin H. The pan-neural bHLH proteins DEADPAN and ASENSE regulate mitotic activity and cdk inhibitor dacapo expression in the Drosophila larval optic lobes. Genesis, 26:77-85, 2000. Li L., Vaessin H.. Pan-neural prospero terminates cell proliferation during Drosophila neurogenesis. Genes&Dev, 14:147- 151, 2000. Burghes AHM, Vaessin H, Chapelle Adl. The land between mendelian and multifactorial inheritance. Science, 293:2213, 2001. Liu, T.-H, Li L, Vaessin H. Transcription of the Drosophila CKI gene dacapo is regulated by a modular array of cis- regulatory sequences. MOD, 112:25-36, 2002. Chandra S, Ahmed A, Vaessin H. The Drosophila IgC2 domain protein friend-of-echinoid, a paralogue of echinoid, limits the number of sensory organ precursors in the wing disc and interacts with the notch signaling pathway. Dev Biol, 256:302-316, 2003. Farooqui T, Robinson K, Vaessin H, Smith BH. Modulation of early olfactory processing by an octopaminergic reinforcement pathway in the honeybee. J Neurosci, 23:5370-5380, 2003. Ahmed A, Chandra S, Magarinos M, Vaessin H. echinoid mutants exhibit neurogenic phenotypes and show synergistic interactions with the Notch signaling pathway. Development, 130:6295-304, 2003. Farooqui, T., Vaessin, H., Smith BH., 2004. Octopamine receptors in the honeybee (Apis mellifera) brain and their disruption by RNA-mediated interference. J Insect Physiol. 50:701-713 Klämbt, C., Vaessin, H. 2005. Neurogenesis in Drosophila: a genetic approach. In: Key experiments in Practical Developmental Biology. Ed. M. Marí-Beffa and J. Knight. Cambridge University Press; pp 282-295. Chandra, S, Zhang, Y, Campbell, C and Vaessin, H. Microarray analysis uncovers a novel role for pnr in the suppression of neurogenic fate in Drosophila wing imaginal discs. Manuscript in preparation. Liu, L, Vaessin, H. Prospero and Cyclin E cooperate in regulation the expression of CKI dacapo and neuronal differentiation /lineage marker Eve-skipped. Manuscript in preparation. B. Research Support Ongoing Research Support National Science Foundation; Developmental Cluster, Neuroscienc Program, “Regulation of neurogenic potential in Drosophila melanogaster” $360,000.-; 7/15/2005 – 7/14/2009 Past Research Support: National Science Foundation; Neuroscience Program; MCB-0091425, "Neuronal Precursor Gene Function in the Regulation of Cell Proliferation and Neuronal Differentiation" $325,085.- ; 9/2001 - 8/2005. National Science Foundation; Neuroscience Program. " Neuronal Precursor Gene Function in Neuronal Lineage Specific Gene Expression”; $300,000.- ; 3/1998 - 2/2002. National Science Foundation; Instrumentation and Instrument Development/Multi-User Instrumentation; A Wide-Field Deconvolution Microscope for Cell and Developmental Biology. PI: Fred Sack; Co-PI’s: C. Beattie, B. Oakley, H. Vaessin, D. Verma.$130,872.- 5/2000 – 4/2003 American Cancer Society (National). “ Role of Nervig, an IgC2 domain protein, in cell adhesion and Signaling”; $240,000.-; 01/1999-12/2002. Ohio Cancer Research Associates. “ Regulation of Cdk inhibitor expression”, $38,386.-; 7/1997 - 6/1999. National Science Foundation; Neuroscience Program. " Neuronal Precursor Gene Function during Drosophila melanogaster Neurogenesis”; $248,000.- , 8/1994 - 9/1997 Alfred P. Sloan Fellow (Neuroscience) ; Alfred P. Sloan Foundation; 30,000.- ; 9/1993 - 9/1997 American Cancer Society, Ohio Division. " Analysis of nervig, a neurogenic gene essential for neurogenesis & epidermogenesis in Drosophila". $19,785.-; 11/1994 -10/1995 Predoctoral Fellowship; Sponsor for Amina Ahmed; from U.A.E. University; U.A.E.; $18,000.-/yr; 1994 - 1998 Interdisciplinary Seed Grant Award (Co-P.I.; together with Dr. Brian Smith, Entomology), “Application of Transgenic Technology to Research on Olfactory Learning in the Honey Bee.” The Ohio State University; $52,000.-; 9/1993-3/1995 Seed Grant Award, "Neuronal precursor gene function in the initiation of neuronal development: Analysis of the Drosophila gene prospero.” The Ohio State University; $14,000; 1992-1993 Postdoctoral Fellowship from Deutsche Forschungsgemeinschaft (DFG); Germany. $45,000.- ; 1987-1989

PHS 398 (Rev. 05/01) Page 3 Biographical Sketch Enter Grant Name Here

1

Name: Desh Pal S. VERMA Current Positions: Professor, Molecular Genetics; Molecular, Cellular and Developmental Biolog; Plant Biology, Plant Pathology and Plant Biotechnology Center,The Ohio State University, Columbus, Ohio USA

EDUCATION B.Sc. - 1962 Biology, Chemistry Agra University M.Sc. - 1964 Botany Agra University Ph.D. - 1970 Plant Science (Physiology and Biochemistry) University of Western Ontario, Canada Post-doctoral Fellow: 1970-72, Molecular Biology, Cancer Institute, Fox Chase Philadelphia, PA, USA Positions Held: Professor, Molecular Genetics/ Biotechnology, Ohio State University, Columbus, OH, USA(1988-present) Associate Director, Biotechnology Center, Ohio State University, Columbus, Ohio, (1988- 1992). Director (1985-1987), Centre for Plant Molecular Biology, McGill University, Montreal, Canada Canadian Pacific Scholar, Agricultural Biotechnology (1985-1986), Biology, McGill University, Montreal, Canada Professor (1982-1987), Biology, McGill University, Montreal, Canada Steacie Fellow - NSERC (1981-1983), McGill University, Montreal Associate Professor (1978-1981), McGill University, Montreal, Canada Assistant Professor (1974-1977), McGill University, Montreal, Canada Research Associate (1972-1974), McGill University, Montreal, Canada Post-doctoral Fellow (1970-1972), Institute for Cancer Research, Philadelphia, PA, USA Research Assistant (1967-1970), University of Western Ontario, London, Ontario, Canada Official Positions: Chairman, Technical Advisory Committee on Secondary Agriculture, Planning Commission, Govt. of India, (2006-present) President, International Society for Molecular Plant-Microbe Interactions (1988 -1992) Treasurer, IS-MPMI (1992-94). Alternate Senator, Ohio State University (2006-2009) Member of Integrative Biology Program Committee (2004-2005) NSF Panel Member, Cellular Biosciences Program (1990) Member, International Commission on Plant Gene Nomenclature (1991- 2001) HONORS AND AWARDS Fellow of the Third World Academy of Sciences, Trieste, Italy (Elected 2004) Honorary Doctorate of Science, Universite de Picarde, France (1996) Fellow of the Royal Society of Canada and a Member of the Academy of Sciences, Canada (Elected 1986)

RESEARCH GRANTS HELD SINCE 2000 1997-2000, NSF, Role of phragmoplastin in cell plate formation in plants. $285,000 2001-2004 $420,000 Molecular Machinery of cell plate formation in plants. 1999-2002 $297,000 USAID Egypt project on osmotolerance in plants 2002-2005 $120,000 USDA A novel protein kinase involved in osmoregulation in plants

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2007-2011 $605,000 NSF Dissecting the TOR Kinase pathway in plants TOR Kinase pathway in Arabidopsis

RESEARCH PUBLICATIONS 1. Hong Z. and D. P. S. Verma (1994) A Phosphatidylinositol 3-kinase is induced during soybean nodule organogenesis and is associated with membrane proliferation. Proc. Nat. Acad. Sci. USA 91: 9617-9621. 2. Peng, X. and D. P. S. Verma (1995) A rice HAL2-like gene encodes a Ca+ sensitive 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase and complements yeast met22 and Escherichia coli cysQ mutations J. Biol. Chem. 270: 29105-29110. 3. Gu X. and D. P. S. Verma (1996) Phragmoplastin, a dynamin homolog, participates in cell plate formation in plants EMBO J. 15: 695-704 4. Zhang Z., Hong Z. and D. P. S. Verma (2000) Phragmoplastin polymerizes in to spiral coiled structures via two self assembly domins J. Biol. Chem. 275: 8779-8784. 5. Hong Z. Delauney AJ, Verma DPS (2001) A cell plate-specific callose synthase and its interaction with phragmoplastin and UDP-glucose transferase. Plant Cell 13, 755-768. 6. Hong Z, Olson J and D. P. S. Verma (2001) A novel UDP glucose transferase interacts with callose synthase and phragmoplastin at the forming cell plate Plant Cell 13, 769-780. 7. Dong X, Hong Z, Sivaramakrishnan M, Mahfouz M, Verma DPS (2005) Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis. Plant J. 42: 315-328. 8. Mahfouz M., Kim S, Delauney A J. and Verma D P S. (2006) Arabidopsis TARGET OF RAPAMYCIN Interacts with RAPTOR, Which Regulates the Activity of S6 Kinase in Response to Osmotic Stress Signal. Plant Cell 18:477-490 9. Dong X, and DPS Verma (2007) Tissue-specific and pathogen-induces expression of various callose synthases in Arabidopsis (Planta, in press)

Books (edited): 12

BIOGRAPHICAL SKETCH

NAME POSITION TITLE Michael Weinstein Associate Professor eRA COMMONS USER NAME weinstein41

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY

(if applicable) University of California, San Diego, LaJolla, Ph.D. 1992 Molecular Genetics California Ohio State University, Columbus Ohio B.S. 1985 Zoology/Chemistry

Positions and Honors 2005-present Associate Professor, The Ohio State University, Department of Molecular Genetics and Division of Human Cancer Genetics, OH 1999-2005 Assistant Professor, Ohio State University, Department of Molecular Genetics and Division of Human Cancer Genetics, OH 1995-1999 Postdoctoral Fellow, Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, MD 1998 Winner, Fellow Award for Research Excellence (FARE) 1996-1999 Instructor, Johns Hopkins University, MD 1992-1995 Postdoctoral Fellow, Children’s Hospital Medical Center, Cincinnati, Ohio 1983-1985 Research Assistant, Ohio State University Selected peer-reviewed publications. (1998-present) 1. Debies M, Waters W, Taffany D, Spahich N, Festing M, Weinstein M. 2007. SARA, a FYVE Domain Containing Protein Linked To TGF-ß Signaling, Is Not Required For Murine Viability and Fertility. Mol Cell Biol. under revision. 2. Liu Y, Lasse S, Bail S,Thompson JC, Kiledjian M, Weinstein M. 2007. Smif/Dcp1a, a constituent of mRNA decapping complexes also thought to function in TGFβ signaling, is essential for mammalian placentogenesis. Dev Biol. Submitted. 3. Pavel E, Zhao W, Powell KA, Weinstein M, Kirschner LS. 2007. Analysis of a new allele of limb deformity (ld) reveals tissue- and age-specific transcriptional effects of the Ld Global Control Region. Int J Dev Biol. 51(4):273-81. 4. Acharyya S, Villalta SA, Bakkar N, Bupha-Intr T, Janssen PM, Carathers M, Li ZW, Beg AA, Ghosh S, Sahenk Z, Weinstein M, Gardner KL, Rafael-Fortney JA, Karin M, Tidball JG, Baldwin AS, Guttridge DC. 2007. Interplay of IKK/NF-kappaB signaling in macrophages and myofibers promotes muscle degeneration in Duchenne muscular dystrophy. J Clin Invest. Apr;117(4):889-901. 5. Dorrance AM, Liu S, Yuan W, Becknell B, Arnoczky KJ, Guimond M, Strout MP, Feng L, Nakamura T, Yu L, Rush LJ, Weinstein M, Leone G, Wu L, Ferketich A, Whitman SP, Marcucci G, Caligiuri MA. 2006. Mll partial tandem duplication induces aberrant Hox expression in vivo via specific epigenetic alterations. J Clin Invest. 116(10):2707-16. 6. Yu J, Wei M, Becknell B, Trotta R, Liu S, Boyd Z, Jaung MS, Blaser BW, Sun J, Benson DM Jr, Mao H, Yokohama A, Bhatt D, Shen L, Davuluri R, Weinstein M, Marcucci G, Caligiuri MA. 2006. Pro- and antiinflammatory cytokine signaling: reciprocal antagonism regulates interferon-gamma production by human natural killer cells. Immunity. 24(5):575-90. 7. Weinstein, M., and Deng, C. X. 2006. Genetic disruptions within the murine genome reveal the numerous roles of the Smad gene family in development, disease, and cancer. Smad signal Transduction: Smads in proliferation, differentiation and disease (Dordrecht, Springer). Editors: Ten Dijke, P. and Heldin, Carl-Henrik. Vol 5, Chapter 8, 151-176. 8. Wei G, Cantemir C, Guo J, Santhanam R, Trimboli A, Weinstein M, Muthusamy M, Yanagisawa M, Man A K, Oshima RG, Leone G, and Ostrowski M C. 2005. Endothelial Cell Autonomous Action of Ets1 and Ets2 is Essential for Embryonic Angiogenesis. Genes and Development, under revision. 9. Satoskar AA, Tanner SM, Weinstein M, Qualman SJ, de la Chapelle A. 2005. Baalc, a marker of mesoderm and muscle. Gene Expr Patterns. 2005 Apr;5(4):463-73. 10. Bartholin L, Powers S E, Melhuish T A, Lasse, S , Weinstein M, and Wotton, D. 2005. TGIF inhibits retinoid signaling during embryonic development. Mol Cell Biol. Feb;26(3):990-1001. 11. Hester M, Thompson J C, Mills J, El-Hodiri H M, and Weinstein M. 2005. Critical dose-dependent functions of BMP-regulated Smads in the development of the mammalian central nervous system. Mol Cellular Biol, 25(11):4683-92. 12. Liu Y, Festing M H, Hester M, Thompson J C, and Weinstein M. 2004. Generation of Novel Conditional and Hypomorphic Alleles of the Smad2 Gene. Genesis 40(2):118. 13. Liu Y, Festing M, Thompson, JC, Hester M, Rankin S, El-Hodiri, H, Zorn, A, and Weinstein M. 2004. Smad2 and Smad3 coordinately regulate endodermal development. Dev Biol 270, 411-426. 14. Cao X., Wei G., Fang H., Guo J., Weinstein, M., Marsh, C. B., Ostrowski, M. C., and S. Tridandapani (2004) The Inositol 3-Phosphatase PTEN Negatively Regulates FcgR Signaling, but Supports TLR4 Signaling in Murine Peritoneal Macrophages. J Immunol. 2004 Apr 15;172(8):4851-7. 15. Weinstein M. (2004). Stationery Flight. Cypress House Press, Ft. Bragg, Ca. 16. Tannehill-Ger, S, Kusewitt, D, Rosol, T, and Weinstein M. The Roles of Smad2 and Smad3 in the Development of Chemically-Induced Skin Tumors in Mice. Vet Pathol. 2004 May;41(3):278-82. 17. De Bruin A, Wu L, Saavedra HI, Wilson P, Yang Y, Rosol TJ, Weinstein M, Robinson ML, Leone G. 2003. Rb function in extraembryonic lineages suppresses apoptosis in the CNS of Rb-deficient mice. Proc Natl Acad Sci U S A 100(11):6546-51. 18. Wu L, de Bruin A, Saavedra H, Starovic M, Trimboli A, Yang Y, Opavska J, Wilson P, Thompson J, Ostrowski M, Rosol T, Woollett L, Weinstein M, Cross J, Robinson M, Leone G. 2003. Extraembryonic Function of Rb Is Essential for Embryonic Development and Viability. Nature 421:942-7. 19. Saavedra HI, Wu L, de Bruin A, Timmers C, Rosol TJ, Weinstein M, Robinson ML, Leone G. 2002. Specificity of E2F1, E2F2, and E2F3 in mediating phenotypes induced by loss of Rb. Cell Growth Differ. May;13(5):215-25. 20. Christensen RN, Weinstein M, Tassava RA. 2002. Expression of fibroblast growth factors 4, 8, and 10 in limbs, flanks, and blastemas of Ambystoma. Dev Dyn. 2002 Mar;223(2):193-203. 21. Christensen RN, Weinstein M, Tassava RA. 2001. Growth factors in regenerating limbs of Ambystoma: Cloning and semi-quantitative RT-PCR expression studies. J Exp Zool. 290(5):529-40. 22. Ito Y, Zhao J, Mogharei A, Shuler CF, Weinstein M, Deng C, Chai Y. 2001. Antagonistic effects of Smad2 versus Smad7 are sensitive to their expression level during tooth development. J Biol Chem. 2001 Sep 13. 23. Weinstein M, S Monga , Y Liu, S. Brodie, Y Tang, C. Li, L. Mishra, and C. Deng. 2001. Smad Proteins and Hepatocyte Growth Factor Control Parallel Regulatory Pathways That Converge on 1-Integrin To Promote Normal Liver Development. Mol. Cell. Biol. 21:5122-5131. 24. Piek E, Ju W, Heyer J, Escalante-Alcalde D, Stewart CL, Weinstein M, Deng C, Kucherlapati R, Boettinger EP, Roberts AB. Functional Characterization of TGF-b Signaling in Smad2- and Smad3- Deficient Fibroblasts. J Biol Chem. 2001. 25. Yu C, F. Wang, M. Kan, C. Jin, R. B. Jones, M. Weinstein, CX. Deng, and W. L. McKeehan. 2000. Elevated Cholesterol Metabolism and Bile Acid Synthesis in Mice Lacking Membrane Tyrosine Kinase Receptor FGFR4. J Biol Chem 275:15482-15489. 26. Weinstein M, X. Yang, and C.Deng. 2000. Functions of mammalian smad genes as revealed by targeted gene disruption in mice. Cytokine Growth Factor Rev. 11:49-58. 27. Ashcroft, G. S., X. Yang, A. Glick, M. Weinstein, J. Letterio, D. E. Mizel, M. Anzano, T. Greenwell-Wild, S. Wahl, C. Deng, and A. B. Roberts. 1999. Mice lacking SMAD3 show accelerated wound healing and an impaired local inflammatory response. Nature Cell Biol. 1: 260-266. 28. X. Yang, L. Castilla, X. Xu, C. Li, J. Gotay, M. Weinstein, P. P. Liu, and C. Deng. 1999. Angiogenesis defects and mesenchymal apoptosis in mice lacking SMAD5. Development 126: 1571-1580. 29. Xu, X., M. Weinstein, C. Li, and C. Deng. 1998. Fibroblast growth factor receptors (FGFRs) and their roles in limb development. Cell and Tissue Res 296: 33-43. 30. Shen, S, Z. Weaver, X. Xu, C. Li, M. Weinstein, L.Chen, X. Guan, T. Reid, and C. Deng. 1998. A mutation of murine Brca1 gene (Brca111) causes g-radiation hypersensitivity and genetic instability. Oncogene 17: 3115-3124. 31. Weinstein, M., X. Yang, C. Li, X. Xu, J. Gotay and C. Deng. 1998. Failure of extraembryonic membrane formation and mesoderm induction in embryos lacking the tumor suppressor smad2. PNAS 95:9378- 9383. 32. Weinstein, M., X. Xu, K. Ohyama, and C. Deng. 1998. Fibroblast Growth Factor Receptor-3 and Receptor-4 Function Cooperatively to Direct Alveogenesis in the Murine Lung. Development 125:3615- 3623.

Research Support.

Active

Completed Title: Functions of the tumor suppressor retinoblastoma during placental and embryonic development PI: Michael Weinstein Dates of Entire Project: 07/01/02-06/30/07 Agency: National Institute of Child Health and Human Development, NIH

The major goals of this project are to determine the role of the Retinoblastoma tumor suppressor in placental development, to examine the impact of the abnormal placental development seen in Rb mutants on the development of Rb mutant embryos, and to examine the genetic regulatory networks that control placentogenesis

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Wharton, Robin Philip Professor of Molecular Genetics & Microbiology eRA COMMONS USER NAME rwharton EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Yale College New Haven, CT B.S. 1974-1978 Biochemistry Harvard University Cambridge, MA Ph.D. 1979-1986 Biochemistry Columbia University New York, NY post-doc 1987-1992 Molecular Genetics A. Positions and Honors Positions and Employment 1978-1979 Churchill Fellow at the MRC Laboratory of Molecular Biology, Cambridge, UK 1979-1986 Graduate Student, Harvard University, Cambridge, MA 1986-1987 Postdoctoral Fellow, Harvard University, Cambridge, MA 1987-1992 Postdoctoral Fellow, Columbia University, New York, NY 1993-2001 Assistant Investigator of HHMI 2001-2004 Associate Investigator of HHMI 2005- Investigator of HHMI 1993-1999 Assistant Professor of Genetics, Duke University Medical Center, NC 1999-2005 Associate Professor of Molecular Genetics and Microbiology, Duke University Medical Center, NC 2005- Professor of Molecular Genetics and Microbiology, Duke University Medical Center, NC Other Experience June, 2007 Ad hoc member of Molecular Genetics C Study Section Honors 1978-1979 Churchill Fellowship 1979-1982 NSF Predoctoral Fellowship 1987-1990 Helen Hay Whitney Postdoctoral Fellowship B. Peer-reviewed publications 1. Sancar, A., Wharton, R., Seltzer, S., Kacinski, B., Clarke, N., and Rupp, W.D. (1981) Identification of the uvrA gene product. JMB 148: 45-62. 2. Wharton, R., Brown, E., and Ptashne, M. (1984) Substituting an alpha-helix switches the sequence- specific DNA interactions of a repressor. Cell 38: 361-369. 3. Wharton, R. and Ptashne, M. (1985) Changing the binding specificity of a repressor by redesigning an alpha-helix. Nature 316: 601-605. 4. Wharton, R. and Ptashne, M. (1986) An alpha-helix determines the DNA-binding specificity of a repressor. TIBS 11: 71-73. 5. Wharton, R. and Ptashne, M. (1987) A new-specificity mutant of 434 repressor that defines an amino acid-base pair contact. Nature 326: 888-891. 6. Wharton, R. and Struhl, G. (1989) Structure of the Drosophila BicaudalD protein and its role in localizing the posterior determinant nanos. Cell 59: 881-892. 7. Wharton, R. and Struhl, G. (1991) RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos. Cell, 67: 955-967. 8. Wharton, R. (1992) Regulated expression from maternal mRNAs in Drosophila. Seminars in Developmental Biology, 3: 391-397. 9. Murata, Y. and Wharton, R.P. (1995) Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos. Cell 80: 747-56. 10. Asano, M., Nevins, J.R., and Wharton, R.P. (1996) Ectopic E2F expression induces S phase and apoptosis in Drosophila imaginal discs. Genes & Dev. 10: 1422-1432. 11. Dahanukar, A., and Wharton, R.P. (1996) The Nanos gradient in Drosophila embryos is generated by translational regulation. Genes & Dev. 10: 2610-2620. 12. Wharton, R.P., Sonoda, J., Lee, T., Patterson, M., and Murata, Y. (1998). The Pumilio RNA-binding domain is also a translational regulator. Molecular Cell 1: 863-872. 13. Asano, M. and Wharton, R.P. (1999). E2F mediates develomental and cell cycle regulation of ORC1 in Drosophila. EMBO J. 9: 2435-2448. 14. Dahanukar, A., Walker, J.A., and Wharton, R.P. (1999). Smaug, a novel RNA-binding protein that operates a translational switch in Drosophila. Molecular Cell 4: 209-218. 15. Sonoda, J. and Wharton, R.P. (1999). Recruitment of Nanos to hunchback mRNA by Pumilio. Genes & Dev. 13: 2704-2712. 16. Verrotti, A.C. and Wharton, R.P. (2000). Nanos interacts with Cup in the female germline of Drosophila. Development 127: 5225-5232. 17. Edwards, T.A., Trinacao, J., Escalante, C.R., Wharton, R.P., and Aggarwal, A.K. (2000). Crystallization and characterization of Pumilio: a novel RNA binding protein. J. Struct. Biol. 132: 251-254. 18. Sonoda, J. and Wharton, R.P. (2001). Drosophila Brain Tumor is a translational repressor. Genes & Dev. 15: 762-773. 19. Edwards, T.A., Pyle, S.E., Wharton, R.P., and Aggarwal A.K. (2001). Structure of Pumilio Reveals Similarity between RNA and Peptide Binding Motifs. Cell, 105: 281-289. 20. Green, J.B., Edwards, T.A., Trincao, J., Escalante, C.R., Wharton, R.P., and Aggarwal, A.K. (2002). Crystallization and characterization of Smaug: a novel RNA binding motif. BBRC 297: 1085. 21. Dean, K. A., Aggarwal, A.K., and Wharton, R.P. (2002). Translational repressors in flies. TIGS 18: 572- 577. 22. Green, J.B., Gardner, C.D., Wharton, R.P., and Aggarwal, A.K. (2003). RNA recognition via the SAM domain of Smaug. Mol. Cell 11: 1537-1548. 23. Edwards, T.A., Wilkinson, B.D., Wharton, R.P., and Aggarwal, A.K. (2003). Model of the Brain tumor- pumilio translation repressor complex. Genes and Dev. 17: 2508-2513. 24. Araki, M., Wharton, R.P., Tan, Z., Yu, H., and Asano, M. (2003). Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila. EMBO J. 22: 6115-6126. 25. Menon, K.P., Sanyal, S., Habara, Y., Sanchez, R., Wharton, R.P., Ramaswami, M., and Zinn, K. (2004). The translational repressor Pumilio regulates presynaptic morphology and controls postsynaptic accumulation of translation factor eIF-4E. Neuron 44: 663-676. 26. Edwards, T.A., Butterwick, J.A., Zeng, L., Gupta, Y.K., Wang, X., Wharton, R. P., Palmer, A.G., Aggarwal, A.K. (2006). Solution structure of the Vts1 SAM domain in the presence of RNA. J. Mol. Biol. 356: 1065-72. 27. Lee, C.-Y., Wilkinson, B.D., Siegrist, S.E., Wharton, R. P., and Doe, C. Q. (2006) Brat is a Miranda cargo protein that promotes neuronal differentiation and inhibits neuroblast self-renewal. Dev. Cell 10: 441-49. 28. Wharton, R.P. and Aggarwal, A.K. (2006). mRNA regulation by Puf domain proteins. Science STKE 354: pe37. 29. Kadyrova, L., Habara, Y., Lee, T.H. and Wharton, R.P. (2007). Translational control of maternal CyclinB mRNA by Nanos in the Drosophila germ line. Development 134: 1519-1527. 30. Song, Y., Lee, T., Fee, L., and Wharton, R.P. (2007). The molecular chaperone Hsp90 is required for mRNA localization in Drosophila melanogaster embryos. Genetics 176: 2213-2222. 31. Gupta, Y.K., Nair, D.T., Wharton, R.P., and Aggarwal, A.K. (2008). Structures of human Pumilio with non-cognate RNAs reveal flipped-out nucleotides. Structure 16: 549-557. C. Research support Ongoing Research Support HHMI Wharton (PI) 2/93-8/08 Subcellular localization and regulated translation of mRNA in Drosophila development. NIH/GM 5 R01 GM62947 Aggarwal (PI) 5/05-4/09 Role: Co-PI Structure and assembly of translation repressor complexes Completed Research Support NIH/GM 5 R01 GM64726 Wharton (PI) 8/02-7/07 Size regulation by NHL domain proteins in Drosophila

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Wu, Jian-Qiu Assistant Professor eRA COMMONS USER NAME WUJQ620 EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Ocean University of China, Qingdao, China B.S. 1989-1993 Biology Institute of Oceanology, CAS, Qingdao, China M.S. 1993-1996 Molecular Biology The University of North Carolina at Chapel Hill Ph.D. 1996-2001 Biology Yale University, New Haven, CT postdoc 11/01-03/06 Cell Biology Yale University, New Haven, CT Res. Sci. 04/06-08/07 Cell Biology

A. Positions and Honors. List in chronological order previous positions, concluding with your present position. List any honors. Include present membership on any Federal Government public advisory committee. Research Experience 1993-1996 Advisors: Dr. C.K. Tseng and Dr. Song Qin at Inst. of Oceanology, Chinese Academy of Sciences, Qingdao, China Master's thesis research on selectable markers for genetic transformation of kelp and regeneration of transgenic kelp. 1996-1997 Rotation in Dr. Ralph S. Quatrano's laboratory at Dept. of Biology, The University of North Carolina at Chapel Hill Screening of Arabidopsis and maize DNA libraries for septins and integrins, and in situ hybridization of actin and myosin mRNAs in Fucus. 1997-2001 Advisor: Dr. John R. Pringle at Dept. of Biology, The University of North Carolina at Chapel Hill Ph.D. dissertation research on roles of alpha-actinin, fimbrin, and septins in cell polarization and cytokinesis in the fission yeast S. pombe. 11/01-03/06 Advisor: Dr. Thomas D. Pollard at Dept. of MCDB, Yale University, New Haven, CT Postdoctoral research on molecular mechanisms of cytokinesis in fission yeast. 04/06-08/07 Advisor: Dr. Thomas D. Pollard at Dept. of MCDB, Yale University, New Haven, CT Associate research scientist on molecular mechanisms of cytokinesis in fission yeast. 08/2007-now Assistant Professor at The Ohio State University, Columbus, OH Molecular mechanisms of cytokinesis.

Members and Honors 1989-1993 Highest University Scholarships 2001-present Member of the American Society for Cell Biology 2004-2005 The Anna Fuller Fund Molecular Oncology Postdoctoral Fellowship

B. Selected peer-reviewed publications (in chronological order). Do not include publications submitted or in preparation. 1. Wu, J.-Q., X.-H. Wang, S. Qin, and C.K. Tseng. 1995. Studies of selectable marker for kelp transformation. Marine Sciences 5: 42-45. [In Chinese] 2. Wu, J.-Q., S. Qin, T. Deng, X.-L. Guo, and C.K. Tseng. 1999. Expression of chloramphenicol acetyltransferase (CAT) gene transferred into Laminaria japonica. Oceanologia et Limnologia Sinica 30: 28-33. [In Chinese]

3. Bähler, J.*, J.-Q. Wu*, M.S. Longtine, N.G. Shah, A. McKenzie III, A.B. Steever, A. Wach, P. Philippsen, and J.R. Pringle. 1998. Heterologous modules for efficient and versatile gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951. *Co-first authors http://www3.interscience.wiley.com/cgi-bin/abstract/3913/ABSTRACT?CRETRY=1&SRETRY=0 4. Wu, J.-Q., J. Bähler, and J.R. Pringle. 2001. Roles of a fimbrin and an α-actinin-like protein in fission yeast cell polarization and cytokinesis. Mol. Biol. Cell 12: 1061-1077. http://www.molbiolcell.org/cgi/content/full/12/4/1061 5. Wu, J.-Q., J.R. Kuhn, D.R. Kovar, and T.D. Pollard. 2003. Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Dev. Cell 5: 723-734. http://www.developmentalcell.com/content/article/abstract?uid=PIIS1534580703003241 6. Kovar, D.R., J.-Q. Wu, and T.D. Pollard. 2005. Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast. Mol. Biol. Cell 16: 2313-2324. http://www.molbiolcell.org/cgi/content/full/16/5/2313 7. Wu, J.-Q. and T.D. Pollard. 2005. Counting cytokinesis proteins globally and locally in fission yeast. Science 310: 310-314. http://www.sciencemag.org/cgi/content/full/310/5746/310 8. Wu, J.-Q., V. Sirotkin, D.K. Kovar, M. Lord, C.C. Beltzner, J.R. Kuhn, and T.D. Pollard. 2006. Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. J. Cell Biol. 174: 391-402. http://www.jcb.org/cgi/content/full/174/3/391 9. Vavylonis*, D, J.-Q. Wu*, S. Hao, B. O’Shaughnesy and T.D. Pollard. 2008. Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast. Science 319: 97-100. *Co-first authors. http://www.sciencemag.org/cgi/content/full/319/5859/97

C. Research Support. Ongoing Research Support 1. American Cancer Society, Ohio Pilot Research Grant 01/01/2008 – 12/31/2008 • Award Name/ID#: 60014673 • Title: Molecular Mechanism of Contractile-Ring Formation during Cytokinesis • Overall goals: The goal of this project is to character the genetic and physical interactions among the proteins in the precursor of contractile ring during cytokinesis. • Role: P.I. 2. American Heart Association, Great Rivers Affiliate 07/01/2008 – 06/30/2010 • Award Name/ID#: 60015862 • Title: Assembly of the Actomyosin Contractile Ring • Overall goals: The goal of this project is to investigate the roles of anillin Mid1p, and actin-bundling protein alpha-actinin Ain1p amd fimbrin Fim1 in the assembly of contractile ring during cytokinesis. • Role: P.I. 3. Ohio Cancer Research Associates 07/01/2008 – 06/30/2010 • Award Name/ID#: 60016307 • Title: Phosphorylation of the Anillin Mid1p by Polo Kinase during Cytokinesis • Overall goals: The goal of this project is to investigate the signaling pathway of the assembly and constriction of the contractile ring, focusing on the phosphorylation of the anillin Mid1p by Polo kinase. • Role: P.I. 4. Basil O’Connor Starter Scholar Research Award 02/01/2009 – 01/31/2011 • Title: Roles of Small GTPase Septins and the Rho GEF Rgf3p in Cytokinesis • Overall goals: The goal of this project is to investigate the roles of small GTPase septin and Rho GEF Rgf3p in late stage of cytokinesis, especially membrane dynamics and cell separation. • Role: P.I. 5. National Institutes of Health 04/01/2009 – 03/31/2014 • Award Name/ID#: R01 GM086546

• Title: Molecular Mechanism of the Contractile-Ring Assembly in Fission Yeast Cytokinesis • Overall goals: The goal of this project is to investigate and distinguish the models of contractile-ring assembly. • Role: P.I.