Table3. DifferentiallyexpressedproteinsidentifiedbyLCMS/MS.ResultsforpairwisemultiplecomparisonusingHolm Sidakmethodarelistedinlast6columns.“↑”indicatessignificantlyupregulated,“↓”significantlydownregulated(p<0.05). F+/+ F+/+ F+/+ S+/+ F/ F/ Spot ProteinIdentification MW pI vs vs vs vs vs vs Function S+/+ S/ F/ S/ S/ S+/+ 9501 Ubiquinolcytochromecreductasecomplexcoreprotein2,mitochondrial 48235 9.3 ↑ ↑ ↑ 8508 Ubiquinolcytochromecreductasecomplexcoreprotein2,mitochondrial 48235 9.3 ↓ ↓ 2505 Ubiquinolcytochromecreductasecomplexcoreprotein1,mitochondrial 52769 5.8 ↓ ↑ ↑ 7006 NADHdehydrogenase[ubiquinone]1alphasubcomplexsubunit5 13360 7.8 ↑ ↓ ↑ ElectronTransport 9303 NADHdehydrogenase[ubiquinone]1alphasubcomplexsubunit4 9327 9.5 ↓ ↓ ↓ Chain 8017 NADHdehydrogenase[ubiquinone]ironsulfurprotein5 12648 9.1 ↑ 6006 CytochromecoxidasesubunitVIa 12483 10.0 ↓ 7214 Electrontransferflavoproteinsubunitbeta 27623 8.3 ↑ ↑ ↑ 7609 ATPsynthasesubunitalpha,mitochondrialprecursor 59753 9.2 ↓ 7709 ATPsynthasesubunitalpha,mitochondrialprecursor 59753 9.2 ↑ ↑ 33 7215 ATPsynthasesubunitalpha,mitochondrialprecursor 59753 9.2 ↑ ↑ ↑ 4110 ATPsynthasesubunitalpha,mitochondrialprecursor 59753 9.2 ↑ ↑ ↑ ↑ ↑ 6103 ATPsynthasesubunitalpha,mitochondrialprecursor 59753 9.2 ↑ ↑ ↑ ATPsynthesis 206 ATPsynthasesubunitbeta,mitochondrialprecursor 56300 5.2 ↑ ↑ ↑ 310 ATPsynthasesubunitbeta,mitochondrialprecursor 56300 5.2 ↑ ↑ ↑ 8324 ATPsynthasegammachain,mitochondrialprecursor 32886 9.1 ↑ ↓ ↓ 1110 ATPsynthaseDchain,mitochondrial 18749 5.5 ↑ ↑ ↑ ↑ 7210 Adenylatekinaseisoenzyme4,mitochondrial 25062 7.0 ↑ ↑ ↑ 7513 Isoform2ofIsocitratedehydrogenase[NADP],mitochondrial 50906 8.9 ↑ ↑ ↑ 7502 IsoformMitochondrialofFumaratehydratase,mitochondrial 54371 9.1 ↑ ↑ ↑ ↑ 7505 IsoformMitochondrialofFumaratehydratase,mitochondrial 54371 9.1 ↑ ↑ ↑ 6515 IsoformMitochondrialofFumaratehydratase,mitochondrial 54371 9.1 ↑ ↑ ↑ CitricAcidCycle 2508 SuccinylCoAligase[ADPforming]betachain,mitochondrial 50114 6.6 ↓ ↓ ↓ 2509 SuccinylCoAligase[ADPforming]betachain,mitochondrial 50114 6.6 ↑ 2512 Isoform1ofSuccinylCoAligase[GDPforming]betachain,mitochondrial 46840 6.6 ↓ ↓ ↓ 4912 Pyruvatecarboxylase 129860 6.3 ↓ ↓

Table3. (Continued). F+/+ F+/+ F+/+ S+/+ F/ F/ Spot ProteinIdentification MW pI vs vs vs vs vs vs Function S+/+ S/ F/ S/ S/ S+/+ 9505 Trifunctionalenzymesubunitbeta,mitochondrialprecursor 51618 9.4 ↓ ↓ ↓ 5410 ShortchainspecificacylCoAdehydrogenase,mitochondrialprecursor 44974 9.0 ↓ ↓ ↓ 5404 ShortchainspecificacylCoAdehydrogenase,mitochondrialprecursor 44947 9.0 ↑ ↑ 4412 ShortchainspecificacylCoAdehydrogenase,mitochondrialprecursor 44947 9.0 ↑ ↑ ↑ ↑ FattyAcidOxidation 4516 MitochondrialacylCoAthioesterase1 49657 6.9 ↑ ↑ ↑ 4310 Delta(3,5)Delta(2,4)dienoylCoAisomerase,mitochondrialprecursor 36118 7.6 ↑ ↑ ↓ ↑ 7214 Electrontransferflavoproteinsubunitbeta 27623 8.3 ↑ ↑ ↑ 4413 NucleosidediphosphatelinkedmoietyXmotif19 40368 6.2 ↓ ↓ ↓ ↓ Dihydrolipoyllysineresidueacetyltransferasecomponentofpyruvate 3701 67942 8.8 dehydrogenasecomplex,mitochondrialprecursor ↓ ↑ ↑ PDC 4502 PyruvatedehydrogenaseE1componentalphasubunit,somaticform, 43232 8.5 mitochondrialprecursor ↑ ↑ ↑ ↑ 34 4810 PropionylCoAcarboxylasealphachain,mitochondrialprecursor 79922 6.8 ↓ ↓ BCAAOxidation 4520 IsovalerylCoAdehydrogenase,mitochondrialprecursor 46325 8.5 ↑ ↑ ↑ ↑ 6221 Glutamatedehydrogenase1,mitochondrialprecursor 61337 8.1 ↑ ↑ ↑ 7711 Cholinedehydrogenase 66331 8.8 ↑ ↑ ↑ 3406 Arginase2,mitochondrialprecursor 38878 6.1 ↑ ↑ ↑ Aminoacid 3505 Ornithineaminotransferase,mitochondrialprecursor 48355 6.2 ↓ ↓ ↓ ↓ metabolism 3506 Ornithineaminotransferase,mitochondrialprecursor 48355 6.2 ↓ ↓ ↓ 3818 Sarcosinedehydrogenase,mitochondrialprecursor 129860 6.3 ↓ ↓ 9202 GTP:AMPphosphotransferasemitochondrial 25426 8.9 ↓ ↓ ↓ Isoform1ofSuccinylCoAligase[GDPforming]betachain,mitochondrial 2512 46840 6.6 precursor ↓ ↓ ↓ Acetaldehyde 4606 Aldehydedehydrogenase,mitochondrialprecursor 56538 7.5 ↓ ↓ ↓ oxidation 4610 Aldehydedehydrogenase,mitochondrialprecursor 56538 7.5 ↓ ↓ ↓ 6704 Aldehydedehydrogenase 57954 8.3 ↓ ↓ ↓ 5503 Glycineamidinotransferase 48297 8.0 ↓ ↓ Creatine 5508 Glycineamidinotransferase,mitochondrialprecursor 48237 8.0 ↓ ↓ ↓ Biosynthesis 5514 Glycineamidinotransferase,mitochondrialprecursor 48297 8.0 ↓ Table3. (Continued). F+/+ F+/+ F+/+ S+/+ F/ F/ Spot ProteinIdentification MW pI vs vs vs vs vs vs Function S+/+ S/ F/ S/ S/ S+/+ 7307 IsoformPlVDAC1ofVoltagedependentanionselectivechannelprotein1 32351 8.6 ↑ ↑ ↑ 8310 IsoformPlVDAC1ofVoltagedependentanionselectivechannelprotein1 32351 8.6 ↑ ↑ ↑ 8316 IsoformPlVDAC1ofVoltagedependentanionselectivechannelprotein1 32351 8.6 ↑ Transport 8320 IsoformMtVDAC1ofVoltagedependentanionselectivechannelprotein 32351 8.6 ↓ ↓ ↑ 2201 Metaxin2 29758 5.4 ↓ ↓ 6105 Superoxidedismutase[Mn],mitochondrialprecursor 24603 8.8 ↑ ↑ ↑ Antioxidants 6108 Sod2Superoxidedismutase[Mn],mitochondrialprecursor 24603 8.8 ↑ ↑ ↑ 2701 Isoform1of60kDaheatshockprotein,mitochondrialprecursor 60955 5.9 ↑ ↓ ↑ 2710 Isoform1of60kDaheatshockprotein,mitochondrialprecursor 60955 5.9 ↑ ↑ ↑ ↑ 2711 Isoform1of60kDaheatshockprotein,mitochondrialprecursor 60955 5.9 ↑ ↓ ↓ Responsetostress 2814 Stress70protein,mitochondrialprecursor 73528 5.9 ↓ ↓ ↓ 35 815 78kDaglucoseregulatedproteinprecursor 72422 5.1 ↓ ↓ 8008 RibonucleaseUK114 14255 8.7 ↑ 3819 Isoform1ofMitochondrialinnermembraneprotein 83900 6.2 ↓ ↓ ↓ 6220 ES1proteinhomolog,mitochondrialprecursor 28090 9.0 ↑ ↓ ↑ 4407 Stomatinlikeprotein2 38385 9.0 ↑ ↓ Other 115 Cytochromeb5 15241 5.0 ↓ ↓ 1809 PDZdomaincontainingprotein1 56499 5.3 ↓ ↓ ↓ 2805 Isoform1ofVacuolarATPsynthasecatalyticsubunitA 68326 5.4 ↓ ↓ ↓ 2001 VacuolarATPsynthasesubunitF 13370 5.5 ↓ ↓ ↓ ↓