Table 3. Differentiallyexpressed proteinsidentified byLC-MS/MS. Resultsfor pairwise multiple comparison using Holm- Sidakmethod are listed in last 6 columns. “↑” indicatessignificantlyupregulated, “↓” significantlydownregulated (p<0.05). F+/+ F+/+ F+/+ S+/+ F-/- F-/- Spot Protein Identification MW pI vs vs vs vs vs vs Function S+/+ S-/- F-/- S-/- S-/- S+/+ 9501 Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial 48235 9.3 ↑ ↑ ↑ 8508 Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial 48235 9.3 ↓ ↓ 2505 Ubiquinol-cytochrome-c reductase complex core protein 1, mitochondrial 52769 5.8 ↓ ↑ ↑ 7006 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 13360 7.8 ↑ ↓ ↑ Electron Transport 9303 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 9327 9.5 ↓ ↓ ↓ Chain 8017 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 12648 9.1 ↑ 6006 Cytochrome c oxidase subunit VI a 12483 10.0 ↓ 7214 Electron transfer flavoprotein subunit beta 27623 8.3 ↑ ↑ ↑ 7609 ATP synthase subunit alpha, mitochondrial precursor 59753 9.2 ↓ 7709 ATP synthase subunit alpha, mitochondrial precursor 59753 9.2 ↑ ↑ 33 7215 ATP synthase subunit alpha, mitochondrial precursor 59753 9.2 ↑ ↑ ↑ 4110 ATP synthase subunit alpha, mitochondrial precursor 59753 9.2 ↑ ↑ ↑ ↑ ↑ 6103 ATP synthase subunit alpha, mitochondrial precursor 59753 9.2 ↑ ↑ ↑ ATP synthesis 206 ATP synthase subunit beta, mitochondrial precursor 56300 5.2 ↑ ↑ ↑ 310 ATP synthase subunit beta, mitochondrial precursor 56300 5.2 ↑ ↑ ↑ 8324 ATP synthase gamma chain, mitochondrial precursor 32886 9.1 ↑ ↓ ↓ 1110 ATP synthase D chain, mitochondrial 18749 5.5 ↑ ↑ ↑ ↑ 7210 Adenylate kinase isoenzyme 4, mitochondrial 25062 7.0 ↑ ↑ ↑ 7513 Isoform 2 of Isocitrate dehydrogenase [NADP], mitochondrial 50906 8.9 ↑ ↑ ↑ 7502 Isoform Mitochondrial of Fumarate hydratase, mitochondrial 54371 9.1 ↑ ↑ ↑ ↑ 7505 Isoform Mitochondrial of Fumarate hydratase, mitochondrial 54371 9.1 ↑ ↑ ↑ 6515 Isoform Mitochondrial of Fumarate hydratase, mitochondrial 54371 9.1 ↑ ↑ ↑ Citric Acid Cycle 2508 Succinyl-CoA ligase [ADP-forming] beta-chain, mitochondrial 50114 6.6 ↓ ↓ ↓ 2509 Succinyl-CoA ligase [ADP-forming] beta-chain, mitochondrial 50114 6.6 ↑ 2512 Isoform 1 of Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial 46840 6.6 ↓ ↓ ↓ 4912 Pyruvate carboxylase 129860 6.3 ↓ ↓ Table 3. (Continued). F+/+ F+/+ F+/+ S+/+ F-/- F-/- Spot Protein Identification MW pI vs vs vs vs vs vs Function S+/+ S-/- F-/- S-/- S-/- S+/+ 9505 Trifunctional enzyme subunit beta, mitochondrial precursor 51618 9.4 ↓ ↓ ↓ 5410 Short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor 44974 9.0 ↓ ↓ ↓ 5404 Short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor 44947 9.0 ↑ ↑ 4412 Short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor 44947 9.0 ↑ ↑ ↑ ↑ Fatty Acid Oxidation 4516 Mitochondrial acyl-CoA thioesterase 1 49657 6.9 ↑ ↑ ↑ 4310 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor 36118 7.6 ↑ ↑ ↓ ↑ 7214 Electron transfer flavoprotein subunit beta 27623 8.3 ↑ ↑ ↑ 4413 Nucleoside diphosphate-linked moiety X motif 19 40368 6.2 ↓ ↓ ↓ ↓ Dihydrolipoyllysine-residue acetyltransferase component of pyruvate 3701 67942 8.8 dehydrogenase complex, mitochondrial precursor ↓ ↑ ↑ PDC 4502 Pyruvate dehydrogenase E1 component alpha subunit, somatic form, 43232 8.5 mitochondrial precursor ↑ ↑ ↑ ↑ 34 4810 Propionyl-CoA carboxylase alpha chain, mitochondrial precursor 79922 6.8 ↓ ↓ BCAA Oxidation 4520 Isovaleryl-CoA dehydrogenase, mitochondrial precursor 46325 8.5 ↑ ↑ ↑ ↑ 6221 Glutamate dehydrogenase 1, mitochondrial precursor 61337 8.1 ↑ ↑ ↑ 7711 Choline dehydrogenase 66331 8.8 ↑ ↑ ↑ 3406 Arginase-2, mitochondrial precursor 38878 6.1 ↑ ↑ ↑ Amino acid 3505 Ornithine aminotransferase, mitochondrial precursor 48355 6.2 ↓ ↓ ↓ ↓ metabolism 3506 Ornithine aminotransferase, mitochondrial precursor 48355 6.2 ↓ ↓ ↓ 3818 Sarcosine dehydrogenase, mitochondrial precursor 129860 6.3 ↓ ↓ 9202 GTP:AMP phosphotransferase mitochondrial 25426 8.9 ↓ ↓ ↓ Isoform 1 of Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial 2512 46840 6.6 precursor ↓ ↓ ↓ Acetaldehyde 4606 Aldehyde dehydrogenase, mitochondrial precursor 56538 7.5 ↓ ↓ ↓ oxidation 4610 Aldehyde dehydrogenase, mitochondrial precursor 56538 7.5 ↓ ↓ ↓ 6704 Aldehyde dehydrogenase 57954 8.3 ↓ ↓ ↓ 5503 Glycine amidinotransferase 48297 8.0 ↓ ↓ Creatine 5508 Glycine amidinotransferase, mitochondrial precursor 48237 8.0 ↓ ↓ ↓ Biosynthesis 5514 Glycine amidinotransferase, mitochondrial precursor 48297 8.0 ↓ Table 3. (Continued). F+/+ F+/+ F+/+ S+/+ F-/- F-/- Spot Protein Identification MW pI vs vs vs vs vs vs Function S+/+ S-/- F-/- S-/- S-/- S+/+ 7307 Isoform Pl-VDAC1 of Voltage-dependent anion-selective channel protein 1 32351 8.6 ↑ ↑ ↑ 8310 Isoform Pl-VDAC1 of Voltage-dependent anion-selective channel protein 1 32351 8.6 ↑ ↑ ↑ 8316 Isoform Pl-VDAC1 of Voltage-dependent anion-selective channel protein 1 32351 8.6 ↑ Transport 8320 Isoform Mt-VDAC1 of Voltage-dependent anion-selective channel protein 32351 8.6 ↓ ↓ ↑ 2201 Metaxin-2 29758 5.4 ↓ ↓ 6105 Superoxide dismutase [Mn], mitochondrial precursor 24603 8.8 ↑ ↑ ↑ Antioxidants 6108 Sod2 Superoxide dismutase [Mn], mitochondrial precursor 24603 8.8 ↑ ↑ ↑ 2701 Isoform 1 of 60 kDa heat shock protein, mitochondrial precursor 60955 5.9 ↑ ↓ ↑ 2710 Isoform 1 of 60 kDa heat shock protein, mitochondrial precursor 60955 5.9 ↑ ↑ ↑ ↑ 2711 Isoform 1 of 60 kDa heat shock protein, mitochondrial precursor 60955 5.9 ↑ ↓ ↓ Response to stress 2814 Stress-70 protein, mitochondrial precursor 73528 5.9 ↓ ↓ ↓ 35 815 78 kDa glucose-regulated protein precursor 72422 5.1 ↓ ↓ 8008 Ribonuclease UK114 14255 8.7 ↑ 3819 Isoform 1 of Mitochondrial inner membrane protein 83900 6.2 ↓ ↓ ↓ 6220 ES1 protein homolog, mitochondrial precursor 28090 9.0 ↑ ↓ ↑ 4407 Stomatin-like protein 2 38385 9.0 ↑ ↓ Other 115 Cytochrome b5 15241 5.0 ↓ ↓ 1809 PDZ domain-containing protein 1 56499 5.3 ↓ ↓ ↓ 2805 Isoform 1 of Vacuolar ATP synthase catalytic subunit A 68326 5.4 ↓ ↓ ↓ 2001 Vacuolar ATP synthase subunit F 13370 5.5 ↓ ↓ ↓ ↓ .