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Rediscover the POWER of Radiometric Detection

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Rediscover the POWER of Radiometric Detection REDISCOVER THE POWER OF RADIOMETRIC DETECTION RADIOMETRIC REAGENTS GUIDE 2010-2011 Your resource for essential research tools you can trust. A Head B Head STILL THE MOST SENSITIVE Radiometric detection remains the standard used by researchers in many scientific applications. Its’ unprecedented sensitivity gives results that technicians and scientists can trust. Table of Contents of Contents Table n NEN Radiochemicals & Radionuclides Reference Guide 32P-Labeled Nucleotides . 4. 33P-Labeled Nucleotides . 5. 35S-Labeled Nucleotides . 6. 35S-Labeled Amino Acids . 7. 125I Labeled Products . 8. 3H- and 14C-Labeled Products . 12. Radionuclides . 14. n Therapeutic Radionuclides . 17. n Radioimmunoassay Kits . 18. n Technical, Safety & Storage Information . 19. n Radionuclide Safe Handling Guides . 21. n Common Radiochemical Measurements & Conversions . 24. n Vial Packaging . 26. n Radiochemical “ABC” Product Listing . 30. n Films, Screens & Accessories . 119. n Dedicated Microplates for Scintillation Counting . 120 n Custom Radiosynthesis & Radiolabeling Services . 121. n SPA Reagents & Technologies . 127. n Radiation Safety Equipment . 131. n Radiometric Detection Instrumentation . 133. n Scintillation Cocktails & Consumables . 137. n GPCR Membrane Guide . 161. Ordering Guide n To Place an Order/Payment Options/Product Availability & Delivery . 171. n Changes or Cancellations/Product Inspection, Credits & ReturnsTechnical Support . 172. n Online Ordering . 173. n Terms & Conditions of Sale . 177. n Licensing Requirements . 179. n Technical Support . 181. www.perkinelmer.com/RadiometricDetection 2 50 YEARS OF SUCCESS For over 50 years PerkinElmer has been a leading supplier of radiochemicals, liquid scintillation cocktails, vials and nuclear counting detection instruments. Today is no different. We have always been committed to NEN Radiochemicals providing you products for all of your radiometric needs and we are still committed today. We are the source for many of the top brands of reagents and instruments for radiometric detection that everyone knows—from NEN® radiochemicals and Scintillation Proximity Assay (SPA) technologies to the MicroBeta2, TopCount, and Tri-Carb® benchtop liquid scintillation analyzers and the WIZARD² automatic gamma counter. These are the systems of choice for all research applications. The Tri-Carb3180 and Quantulus are the best in class for ultra low level environmental monitoring and testing. Radiometric detection is far more than reagents and instruments. You need scintillation cocktails, microplates, vials, film, safety equipment, service and the know how to use these techniques. PerkinElmer delivers what you need to be successful. At PerkinElmer, we’re taking action to improve the health and safety of people and their environment. Engaged in a proactive fight against illness, contamination and threats to our well-being, PerkinElmer conceives and delivers scientific solutions and components to meet our society’s ever-changing needs.We’re committed to transforming risk into safety, mystery into knowledge and ideas into action for a healthier today and a better tomorrow. www.perkinelmer.com/radiometricdetection 3 NEN Radiochemicals Reference Guide Fresh Lot Calendar: www.perkinelmer.com/Toolkit 32 P-Labeled Nucleotides NEN Radiochemicals Fresh Lot Half Life Precalibration Package Sizes Packaging Every Thursday 14.3 days 9 days 100 µCi Shipped in a choice of blue plastic 250 µCi (BLU prefixes) or lead-lined grey 500 µCi plastic (NEG prefixes) packaging. 1 mCi Shipment in lead is required only for 32P quantities above 1.5 mCi. Help minimize hazardous waste: order lead- free packaging whenever feasible. 32P-Labeled Compound Specific Activity Concentration Product Numbers [Ci/mmol] [mCi/mL] [µM] Color-coded green Shipped in dry ice. EasyTides.® Store at -20 °C. Ready to use. Shipped ambient. Store at 2–8 °C. ATP, [a-32P]- 800 10 12.5 ­— BLU003X/NEG003X 3000 10 3.3 BLU503H/NEG003H BLU003H/NEG003H CTP, [a-32P]- 800 10 12.5 BLU508X/NEG508X BLU008X/NEG008X 3000 10 3.3 BLU508H/NEG508H BLU008H/NEG008H GTP, [a-32P]- 800 10 12.5 — BLU006X/NEG006X 3000 10 3.3 BLU506H/NEG506H BLU006H/NEG006H UTP, [a-32P]- 800 10 12.5 BLU507X/NEG507X BLU007X/NEG007X 800 20 25 BLU507T/NEG507T 800 40 50 BLU507C/NEG507C BLU007C/NEG007C 3000 10 3.3 BLU507H/NEG507H BLU007H/NEG007H 6000 40 6.7 BLU507Z/NEG507Z BLU007Z/NEG007Z dATP, [a-32P]- 800 10 12.5 BLU512A/NEG512A — 3000 10 3.3 BLU512H/NEG512H BLU012H/NEG012H 6000 20 3.3 BLU512Z/NEG512Z BLU012Z/NEG012Z dCTP, [a-32P]- 800 10 12.5 BLU513A/NEG513A BLU013A/NEG013A 3000 10 3.3 BLU513H/NEG513H BLU013H/NEG013H 6000 20 3.3 BLU513Z/NEG513Z BLU013Z/NEG013Z dGTP, [a-32P]- 3000 10 3.3 BLU514H/NEG514H — 6000 20 3.3 BLU514Z/NEG514Z BLU014Z/NEG014Z dTTP, [a-32P]- 800 10 12.5 BLU505A/NEG505A BLU005A/NEG005A 3000 10 3.3 BLU505H/NEG505H BLU005H/NEG005H 3’dATP, [a-32P]- 5000 10 2 — BLU026/NEG026 ATP, [g-32P]- 10 2 200 — BLU002/NEG002 3000 5 1.7 BLU502H/NEG502H BLU002H/NEG002H 3000 10 3.3 BLU502A/NEG502A BLU002A/NEG002A 6000 10 1.7 BLU502Z/NEG502Z BLU002Z/NEG002Z 6000 150 25 — NEG035C GTP, [g-32P]- 6000 10 1.7 BLU504Z/NEG504Z BLU004Z/NEG004Z NAD, [32P]- 800 5 6.3 — BLU023X/NEG023X pCp, [5’-32P]- 3000 10 3.3 — BLU019A/NEG019A New products being added regularly. Visit www.perkinelmer.com/NewProducts for details. www.perkinelmer.com/RadiometricDetection 4 Fresh Lot Calendar: www.perkinelmer.com/Toolkit 33P-Labeled Nucleotides Fresh Lot Half Life Precalibration Package Sizes Packaging Every Other Friday 25.4 days 13 days 100 µCi Shipped in white plastic, available in *Every Friday 250 µCi lead-lined grey plastic upon request. 1 mCi 33P-Labeled Compound Specific Activity Concentration Product Numbers [Ci/mmol] [mCi/mL] [µM] Color-coded amber gold Shipped in dry ice. ® EasyTides . Store at -20 °C. Ready to use. Shipped ambient. NEN Radiochemicals Store at 2–8 °C. ATP, [a-33P]- 3000 10 3.3 NEG603H — CTP, [a-33P]- 3000 10 3.3 NEG608H — GTP, [a-33P]- 3000 10 3.3 NEG606H — UTP, [a-33P]- 3000 10 3.3 NEG607H NEG307H dATP, [a-33P]- 3000 10 3.3 NEG612H NEG312H dCTP, [a-33P]- 3000 10 3.3 NEG613H NEG313H dTTP, [a-33P]- 3000 10 3.3 NEG605H — ATP, [g-33P]- 3000 10 3.3 NEG602H NEG302H 3000 10 3.3 NEG602K* — New products being added regularly. Visit www.perkinelmer.com/NewProducts for details. 5 Fresh Lot Calendar: www.perkinelmer.com/Toolkit 35 S-Labeled Nucleotides NEN Radiochemicals Fresh Lot Half Life Precalibration Package Sizes Packaging First Tuesday each month 87.4 days 5 weeks 100 µCi Shipped in yellow plastic, available in 250 µCi lead-lined grey plastic upon request. 1 mCi 35S-Labeled Compound Specific Activity Concentration Product Numbers [Ci/mmol] [mCi/mL] [µM] Color-coded blue Shipped frozen. EasyTides. Store at -20 °C. Ready to use. Shipped ambient. Store at 2–8 °C. ATPaS, [35S]- 1250 12.5 10 — NEG033H CTPaS, [35S]- 1250 12.5 10 — NEG064H 1250 70 56 — NEG064C UTPaS, [35S]- 800 40 50 — NEG039C 1250 12.5 10 NEG739H NEG039H dATPaS, [35S]- 500 10 20 — NEG034S 1250 12.5 10 NEG734H NEG034H ATPgS, [35S]- 25–100 12.5 125–500 — NEG027 1250 12.5 10 — NEG027H GTPgS, [35S]- 1250 12.5 10 — NEG030H 1250 12.5 10 — NEG030X PAPS 3’, [35S]- 1–3 0.5 167–500 — NEG010 To learn more about our compatible instruments, cocktails, film and membranes, please visit www.perkinelmer.com/RadiometricDetection. www.perkinelmer.com/RadiometricDetection 6 Fresh Lot Calendar: www.perkinelmer.com/Toolkit 35S-Labeled Amino Acids Fresh Mondays, Every Three Weeks Product Name Molecular Formula & Labeling Position Specific Activity [Ci/mmol] Activity Concentration [mCi/mL] Product No. 35 Cysteine, L-[ S]- HSCH2CH(NH2)CO2H >1000 11 NEG022T Packaged in 10 mM DTT. EXPRE35S35S Protein Labeling N/A >1000 11 NEG772 Mix, [35S]-, (EasyTag™) A mixture of approximately 73% [35S]-methionine and 22% L-[35S]-cysteine prepared directly from the hydrolysate of E. coli grown in the presence of 35 carrier-free SO4 with added stabilizer. Packaged in a stabilized aqueous solution. EXPRE35S35S Protein N/A >1000 11 NEG072 NEN Radiochemicals Labeling Mix, [35S]- A mixture of approximately 73% [35S]-methionine and 22% L-[35S]-cysteine prepared directly from the hydrolysate of E. coli grown in the presence of 35 carrier-free SO4. Packaged in 50 mM tricine (pH 7.4) and 10 mM 2-mercaptoethanol. 35 Methionine, L-[ S]- CH3S(CH2)2CH(NH2)CO2H >600 31.5 NEG009L Cell Labeling Grade Packaged in 10 mM 2-mercaptoethanol. 35 Methionine, L-[ S]- CH3S(CH2)2CH(NH2)CO2H >800 11 NEG009H Packaged in 10 mM 2-mercaptoethanol. 35 Methionine, L-[ S]-, (EasyTag) CH3S(CH2)2CH(NH2)CO2H >1000 10.2 NEG709A Packaged in a stabilized aqueous solution with blue dye. 35 Methionine, L-[ S]- CH3S(CH2)2CH(NH2)CO2H >1000 10.2 NEG009A Packaged in 50 mM tricine (pH 7.4) and 10 mM 2-mercaptoethanol. 35 Methionine, L-[ S]- CH3S(CH2)2CH(NH2)CO2H >1000 11 NEG009T Packaged in 10 mM 2-mercaptoethanol. 35 Methionine, L-[ S]- CH3S(CH2)2CH(NH2)CO2H >1000 43.3 NEG009C Packaged in 50 mM tricine (pH 7.4) and 10 mM 2-mercaptoethanol. New products being added regularly. Visit www.perkinelmer.com/NewProducts for details. 7 Fresh Lot Calendar: www.perkinelmer.com/Toolkit 125 I-Labeled Products NEN Radiochemicals Product Product No. A-85380, [125I]- NEX413 AB-MECA, [125I]-, (4-amino-3-[125I]iodobenzyl-5’-N-) NEX312 ACTH, (1-39) Tyr2-[125I]- NEX342 ACTH, (1-39) Tyr23-[125I]- NEX165 Adenosine 3’,5’-cyclic phosphoric acid, 2’-O-succinyl [125I]-iodotyrosine methyl ester NEX130 Adrenomedullin, [125I]Tyr-, (human, 13–52) NEX385 Adrenomedullin (Rat), [125I]- NEX427 AGRP (Ac-87-132), [125I]-, (human, synthetic) NEX372 Albumin, Bovine
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