Anti-BCKDHA Antibody (ARG57384)

Product datasheet [email protected]

ARG57384 Package: 100 μl anti-BCKDHA antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes BCKDHA

Tested Reactivity Hu, Ms, Rat

Tested Application WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name BCKDHA

Antigen Species Human

Immunogen Recombinant Protein of Human BCKDHA.

Conjugation Un-conjugated

Alternate Names EC 1.2.4.4; MSUD1; MSU; BCKDH E1-alpha; OVD1A; BCKDE1A; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial

Application Instructions

Application table Application Dilution

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control DU145

Calculated Mw 50 kDa

Properties

Form Liquid

Purification Affinity purification with immunogen.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/2 Bioinformation

Gene Symbol BCKDHA

Gene Full Name branched chain keto acid dehydrogenase E1, alpha polypeptide

Background The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched- chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). [UniProt]

Images

ARG57384 anti-BCKDHA antibody WB image

Western blot: DU145 cell lysate stained with ARG57384 anti- BCKDHA antibody.