Anti-BCKDHA Antibody (ARG57384)

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Product datasheet [email protected] ARG57384 Package: 100 μl anti-BCKDHA antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes BCKDHA Tested Reactivity Hu, Ms, Rat Tested Application WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name BCKDHA Antigen Species Human Immunogen Recombinant Protein of Human BCKDHA. Conjugation Un-conjugated Alternate Names EC 1.2.4.4; MSUD1; MSU; BCKDH E1-alpha; OVD1A; BCKDE1A; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial Application Instructions Application table Application Dilution WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control DU145 Calculated Mw 50 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. www.arigobio.com 1/2 Bioinformation Gene Symbol BCKDHA Gene Full Name branched chain keto acid dehydrogenase E1, alpha polypeptide Background The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched- chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). [UniProt] Images ARG57384 anti-BCKDHA antibody WB image Western blot: DU145 cell lysate stained with ARG57384 anti- BCKDHA antibody. www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).

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